5 methyl dctp  (Jena Bioscience)


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    Name:
    5 Methyl dCTP
    Description:
    5 methylated DNA probes can be used as methylation reference fragment 1 2 or for pull down of 5 hmC binding proteins from cellular lysate 3
    Catalog Number:
    nu-1125l
    Price:
    253.75
    Applications:
    Incorporation into DNA by - PCR with Taq polymerase in-house data, [1-2]
    Purity:
    ≥ 95 % (HPLC)
    Category:
    Nucleotides Nucleosides
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    Structured Review

    Jena Bioscience 5 methyl dctp
    5 methylated DNA probes can be used as methylation reference fragment 1 2 or for pull down of 5 hmC binding proteins from cellular lysate 3
    https://www.bioz.com/result/5 methyl dctp/product/Jena Bioscience
    Average 85 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    5 methyl dctp - by Bioz Stars, 2020-08
    85/100 stars

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    Related Articles

    Sequencing:

    Article Title: Transcriptome analysis of the bloodstream stage from the parasite Trypanosoma vivax
    Article Snippet: .. Library construction and sequencing Double-stranded cDNA was generated from 25 μg of total RNA using a SuperScript II Double-Stranded cDNA Synthesis Kit (Invitrogen) according to the manufacturer’s instructions, except for oligonucleotide used for first strand synthesis and 5-methyl-dCTP (Jena Biosciences) instead of dCTP. ..

    Concentration Assay:

    Article Title: A versatile non-radioactive assay for DNA methyltransferase activity and DNA binding
    Article Snippet: .. Upon addition of 0.05 u/µl Klenow fragment (NEB, Germany), dTTP, dGTP, dATP (PeqLab, Germany) at 1 mM final concentration, and either CTP at 1 mM, 5-aza-dCTP or 5-methyl-dCTP at 50 µM (Jena Bioscience, Germany), the Fill-In oligonucleotide was extended to produce either unmethylated, hemimethylated or fully methylated canonical DNA substrates or un- or hemimethylated suicide DNA substrates containing 5-aza-dC at the CpG site. .. 5-aza-dC containing suicide DNA substrates are referred to as ‘trapping substrates’ and DNA substrates not containing 5-aza-dC as ‘binding substrates’.

    Generated:

    Article Title: Transcriptome analysis of the bloodstream stage from the parasite Trypanosoma vivax
    Article Snippet: .. Library construction and sequencing Double-stranded cDNA was generated from 25 μg of total RNA using a SuperScript II Double-Stranded cDNA Synthesis Kit (Invitrogen) according to the manufacturer’s instructions, except for oligonucleotide used for first strand synthesis and 5-methyl-dCTP (Jena Biosciences) instead of dCTP. ..

    Methylation:

    Article Title: A versatile non-radioactive assay for DNA methyltransferase activity and DNA binding
    Article Snippet: .. Upon addition of 0.05 u/µl Klenow fragment (NEB, Germany), dTTP, dGTP, dATP (PeqLab, Germany) at 1 mM final concentration, and either CTP at 1 mM, 5-aza-dCTP or 5-methyl-dCTP at 50 µM (Jena Bioscience, Germany), the Fill-In oligonucleotide was extended to produce either unmethylated, hemimethylated or fully methylated canonical DNA substrates or un- or hemimethylated suicide DNA substrates containing 5-aza-dC at the CpG site. .. 5-aza-dC containing suicide DNA substrates are referred to as ‘trapping substrates’ and DNA substrates not containing 5-aza-dC as ‘binding substrates’.

    Polymerase Chain Reaction:

    Article Title: Characterization of PvuRts1I endonuclease as a tool to investigate genomic 5-hydroxymethylcytosine
    Article Snippet: .. Reference DNA fragments containing exclusively hm C, m C or unmodified cytosine residues were prepared by PCR using 5-hydroxymethyl-dCTP (Bioline GmbH), 5-methyl-dCTP (Jena Bioscience GmbH) and dCTP, respectively. .. T4 phage DNA template, Phusion HF DNA Polymerase (Finnzymes) and primer 5′-GTG AAG TAA GTA ATA AAT GGA TTG-3′, which does not contain cytosine residues, were used for amplification of all reference DNA fragments.

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  • 85
    Jena Bioscience 5 methyl dctp
    5 Methyl Dctp, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 85/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/5 methyl dctp/product/Jena Bioscience
    Average 85 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    5 methyl dctp - by Bioz Stars, 2020-08
    85/100 stars
      Buy from Supplier

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