n6 methyl atp  (Jena Bioscience)


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  • 93
    Name:
    N6 Methyl ATP
    Description:

    Catalog Number:
    NU-1101L
    Price:
    203.91
    Category:
    Nucleotides Nucleosides
    Size:
    5 x 10 µl
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    Structured Review

    Jena Bioscience n6 methyl atp
    Potential routes for cellular production and metabolism of <t>N6-methyl-dATP</t> and <t>N6-methyl-ATP.</t> N6-methyl-dATP and N6-methyl-ATP may be produced from N6-methyl-dAMP and N6-methyl-AMP formed upon DNA and RNA degradation, respectively. This may occur through the consecutive actions of adenylate kinase ( AK ) and nucleoside diphosphate kinase or through nonspecific methylation by S -adenosylmethionine ( SAM ), the N6-adenosine-methyltransferase METTL3 or N6-adenine–specific DNA methyltransferase 1 ( N6AMT1 ). N6-methyl-dATP and N6-methyl-ATP are hydrolyzed by MTH1 to their corresponding monophosphates and further metabolized by ADAL1 to dIMP and IMP that can then enter the nucleotide salvage pathway. Abbreviations used in the figure: NDPK , nucleoside diphosphate kinase; RNR, ribonucleotide reductase; METTL3, N6-adenosine methyltransferase; N6AMT1, N6-adenine-specific DNA methyltransferase 1.

    https://www.bioz.com/result/n6 methyl atp/product/Jena Bioscience
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    n6 methyl atp - by Bioz Stars, 2021-06
    93/100 stars

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    1) Product Images from "MutT homologue 1 (MTH1) removes N6-methyl-dATP from the dNTP pool"

    Article Title: MutT homologue 1 (MTH1) removes N6-methyl-dATP from the dNTP pool

    Journal: The Journal of Biological Chemistry

    doi: 10.1074/jbc.RA120.012636

    Potential routes for cellular production and metabolism of N6-methyl-dATP and N6-methyl-ATP. N6-methyl-dATP and N6-methyl-ATP may be produced from N6-methyl-dAMP and N6-methyl-AMP formed upon DNA and RNA degradation, respectively. This may occur through the consecutive actions of adenylate kinase ( AK ) and nucleoside diphosphate kinase or through nonspecific methylation by S -adenosylmethionine ( SAM ), the N6-adenosine-methyltransferase METTL3 or N6-adenine–specific DNA methyltransferase 1 ( N6AMT1 ). N6-methyl-dATP and N6-methyl-ATP are hydrolyzed by MTH1 to their corresponding monophosphates and further metabolized by ADAL1 to dIMP and IMP that can then enter the nucleotide salvage pathway. Abbreviations used in the figure: NDPK , nucleoside diphosphate kinase; RNR, ribonucleotide reductase; METTL3, N6-adenosine methyltransferase; N6AMT1, N6-adenine-specific DNA methyltransferase 1.
    Figure Legend Snippet: Potential routes for cellular production and metabolism of N6-methyl-dATP and N6-methyl-ATP. N6-methyl-dATP and N6-methyl-ATP may be produced from N6-methyl-dAMP and N6-methyl-AMP formed upon DNA and RNA degradation, respectively. This may occur through the consecutive actions of adenylate kinase ( AK ) and nucleoside diphosphate kinase or through nonspecific methylation by S -adenosylmethionine ( SAM ), the N6-adenosine-methyltransferase METTL3 or N6-adenine–specific DNA methyltransferase 1 ( N6AMT1 ). N6-methyl-dATP and N6-methyl-ATP are hydrolyzed by MTH1 to their corresponding monophosphates and further metabolized by ADAL1 to dIMP and IMP that can then enter the nucleotide salvage pathway. Abbreviations used in the figure: NDPK , nucleoside diphosphate kinase; RNR, ribonucleotide reductase; METTL3, N6-adenosine methyltransferase; N6AMT1, N6-adenine-specific DNA methyltransferase 1.

    Techniques Used: Produced, Methylation

    Kinetic characterization of MTH1-catalyzed hydrolysis of dATP, N6-methyl-dATP, N6-methyl-ATP, dGTP, and O6-methyl-dGTP. A, substrate saturation curves of MTH1 (1 n m ) were produced using MTH1 reaction buffer (pH 7.5). Initial rates were determined at dATP concentrations varied between 0 and 200 μ m and between 0 and 300 μ m for N6-methyl-dATP and N6-methyl-ATP, respectively, and for dGTP and O6-methyl-dGTP ( B ) using 0–300 and 0–200 μ m , respectively. Formed PP i was detected using PPiLight TM Inorganic Pyrophosphate Assay (Lonza) and the assay signal was converted to concentration of PP i by including a PP i standard curve on the assay plate.
    Figure Legend Snippet: Kinetic characterization of MTH1-catalyzed hydrolysis of dATP, N6-methyl-dATP, N6-methyl-ATP, dGTP, and O6-methyl-dGTP. A, substrate saturation curves of MTH1 (1 n m ) were produced using MTH1 reaction buffer (pH 7.5). Initial rates were determined at dATP concentrations varied between 0 and 200 μ m and between 0 and 300 μ m for N6-methyl-dATP and N6-methyl-ATP, respectively, and for dGTP and O6-methyl-dGTP ( B ) using 0–300 and 0–200 μ m , respectively. Formed PP i was detected using PPiLight TM Inorganic Pyrophosphate Assay (Lonza) and the assay signal was converted to concentration of PP i by including a PP i standard curve on the assay plate.

    Techniques Used: Produced, Pyrophosphate Assay, Concentration Assay

    MTH1 activity with N6-methyl-dATP and N6-methyl-ATP compared with dATP. Activity of 1 and 5 n m MTH1 was tested with 50 μ m N6-methyl-dATP, dATP, ATP, and N6-methyl-ATP in MTH1 reaction buffer (pH 8.0) at 22 °C. Reaction time was 30 min and 0.2 units/ml of PPase was used to generate P i from produced PP i . P i was detected by addition of malachite green reagent followed by measurement of the absorbance at 630 nm. Controls with PPase only was included and background signal was subtracted from the assay data. A P i standard curve was included on the plate enabling determination of the concentration of formed PP i . Graph shows mean ± S.D. from one experiment performed in quadruplicate.
    Figure Legend Snippet: MTH1 activity with N6-methyl-dATP and N6-methyl-ATP compared with dATP. Activity of 1 and 5 n m MTH1 was tested with 50 μ m N6-methyl-dATP, dATP, ATP, and N6-methyl-ATP in MTH1 reaction buffer (pH 8.0) at 22 °C. Reaction time was 30 min and 0.2 units/ml of PPase was used to generate P i from produced PP i . P i was detected by addition of malachite green reagent followed by measurement of the absorbance at 630 nm. Controls with PPase only was included and background signal was subtracted from the assay data. A P i standard curve was included on the plate enabling determination of the concentration of formed PP i . Graph shows mean ± S.D. from one experiment performed in quadruplicate.

    Techniques Used: Activity Assay, Produced, Concentration Assay

    Related Articles

    Activity Assay:

    Article Title: MutT homologue 1 (MTH1) removes N6-methyl-dATP from the dNTP pool
    Article Snippet: E. coli MutT was produced as described in Ref. and NUDT1 from the plant A. thaliana (atNUDT1) as performed in Ref. . .. MTH1 activity assay Activity of MTH1 (5 or 1 nm ) with 50 μm dATP (Promega), N6-methyl-dATP (Jena Bioscience), ATP (Promega), and N6-methyl-ATP (Jena Bioscience) was assayed in MTH1 reaction buffer (Tris acetate, pH 8.0, 40 mm sodium chloride, 10 mm magnesium acetate). ..

    other:

    Article Title: Structure and ligand binding of the ADP-binding domain of the NAD+ riboswitch
    Article Snippet: N 6-methylATP (NU-1101L) was obtained from Jena Bioscience.

    Article Title: Structure and ligand binding of the ADP-binding domain of the NAD+ riboswitch.
    Article Snippet: N6methylATP (NU-1101L) was obtained from Jena Bioscience.

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    Jena Bioscience n6 methyl atp
    Potential routes for cellular production and metabolism of <t>N6-methyl-dATP</t> and <t>N6-methyl-ATP.</t> N6-methyl-dATP and N6-methyl-ATP may be produced from N6-methyl-dAMP and N6-methyl-AMP formed upon DNA and RNA degradation, respectively. This may occur through the consecutive actions of adenylate kinase ( AK ) and nucleoside diphosphate kinase or through nonspecific methylation by S -adenosylmethionine ( SAM ), the N6-adenosine-methyltransferase METTL3 or N6-adenine–specific DNA methyltransferase 1 ( N6AMT1 ). N6-methyl-dATP and N6-methyl-ATP are hydrolyzed by MTH1 to their corresponding monophosphates and further metabolized by ADAL1 to dIMP and IMP that can then enter the nucleotide salvage pathway. Abbreviations used in the figure: NDPK , nucleoside diphosphate kinase; RNR, ribonucleotide reductase; METTL3, N6-adenosine methyltransferase; N6AMT1, N6-adenine-specific DNA methyltransferase 1.
    N6 Methyl Atp, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/n6 methyl atp/product/Jena Bioscience
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    n6 methyl atp - by Bioz Stars, 2021-06
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    Potential routes for cellular production and metabolism of N6-methyl-dATP and N6-methyl-ATP. N6-methyl-dATP and N6-methyl-ATP may be produced from N6-methyl-dAMP and N6-methyl-AMP formed upon DNA and RNA degradation, respectively. This may occur through the consecutive actions of adenylate kinase ( AK ) and nucleoside diphosphate kinase or through nonspecific methylation by S -adenosylmethionine ( SAM ), the N6-adenosine-methyltransferase METTL3 or N6-adenine–specific DNA methyltransferase 1 ( N6AMT1 ). N6-methyl-dATP and N6-methyl-ATP are hydrolyzed by MTH1 to their corresponding monophosphates and further metabolized by ADAL1 to dIMP and IMP that can then enter the nucleotide salvage pathway. Abbreviations used in the figure: NDPK , nucleoside diphosphate kinase; RNR, ribonucleotide reductase; METTL3, N6-adenosine methyltransferase; N6AMT1, N6-adenine-specific DNA methyltransferase 1.

    Journal: The Journal of Biological Chemistry

    Article Title: MutT homologue 1 (MTH1) removes N6-methyl-dATP from the dNTP pool

    doi: 10.1074/jbc.RA120.012636

    Figure Lengend Snippet: Potential routes for cellular production and metabolism of N6-methyl-dATP and N6-methyl-ATP. N6-methyl-dATP and N6-methyl-ATP may be produced from N6-methyl-dAMP and N6-methyl-AMP formed upon DNA and RNA degradation, respectively. This may occur through the consecutive actions of adenylate kinase ( AK ) and nucleoside diphosphate kinase or through nonspecific methylation by S -adenosylmethionine ( SAM ), the N6-adenosine-methyltransferase METTL3 or N6-adenine–specific DNA methyltransferase 1 ( N6AMT1 ). N6-methyl-dATP and N6-methyl-ATP are hydrolyzed by MTH1 to their corresponding monophosphates and further metabolized by ADAL1 to dIMP and IMP that can then enter the nucleotide salvage pathway. Abbreviations used in the figure: NDPK , nucleoside diphosphate kinase; RNR, ribonucleotide reductase; METTL3, N6-adenosine methyltransferase; N6AMT1, N6-adenine-specific DNA methyltransferase 1.

    Article Snippet: MTH1 activity assay Activity of MTH1 (5 or 1 nm ) with 50 μm dATP (Promega), N6-methyl-dATP (Jena Bioscience), ATP (Promega), and N6-methyl-ATP (Jena Bioscience) was assayed in MTH1 reaction buffer (Tris acetate, pH 8.0, 40 mm sodium chloride, 10 mm magnesium acetate).

    Techniques: Produced, Methylation

    Kinetic characterization of MTH1-catalyzed hydrolysis of dATP, N6-methyl-dATP, N6-methyl-ATP, dGTP, and O6-methyl-dGTP. A, substrate saturation curves of MTH1 (1 n m ) were produced using MTH1 reaction buffer (pH 7.5). Initial rates were determined at dATP concentrations varied between 0 and 200 μ m and between 0 and 300 μ m for N6-methyl-dATP and N6-methyl-ATP, respectively, and for dGTP and O6-methyl-dGTP ( B ) using 0–300 and 0–200 μ m , respectively. Formed PP i was detected using PPiLight TM Inorganic Pyrophosphate Assay (Lonza) and the assay signal was converted to concentration of PP i by including a PP i standard curve on the assay plate.

    Journal: The Journal of Biological Chemistry

    Article Title: MutT homologue 1 (MTH1) removes N6-methyl-dATP from the dNTP pool

    doi: 10.1074/jbc.RA120.012636

    Figure Lengend Snippet: Kinetic characterization of MTH1-catalyzed hydrolysis of dATP, N6-methyl-dATP, N6-methyl-ATP, dGTP, and O6-methyl-dGTP. A, substrate saturation curves of MTH1 (1 n m ) were produced using MTH1 reaction buffer (pH 7.5). Initial rates were determined at dATP concentrations varied between 0 and 200 μ m and between 0 and 300 μ m for N6-methyl-dATP and N6-methyl-ATP, respectively, and for dGTP and O6-methyl-dGTP ( B ) using 0–300 and 0–200 μ m , respectively. Formed PP i was detected using PPiLight TM Inorganic Pyrophosphate Assay (Lonza) and the assay signal was converted to concentration of PP i by including a PP i standard curve on the assay plate.

    Article Snippet: MTH1 activity assay Activity of MTH1 (5 or 1 nm ) with 50 μm dATP (Promega), N6-methyl-dATP (Jena Bioscience), ATP (Promega), and N6-methyl-ATP (Jena Bioscience) was assayed in MTH1 reaction buffer (Tris acetate, pH 8.0, 40 mm sodium chloride, 10 mm magnesium acetate).

    Techniques: Produced, Pyrophosphate Assay, Concentration Assay

    MTH1 activity with N6-methyl-dATP and N6-methyl-ATP compared with dATP. Activity of 1 and 5 n m MTH1 was tested with 50 μ m N6-methyl-dATP, dATP, ATP, and N6-methyl-ATP in MTH1 reaction buffer (pH 8.0) at 22 °C. Reaction time was 30 min and 0.2 units/ml of PPase was used to generate P i from produced PP i . P i was detected by addition of malachite green reagent followed by measurement of the absorbance at 630 nm. Controls with PPase only was included and background signal was subtracted from the assay data. A P i standard curve was included on the plate enabling determination of the concentration of formed PP i . Graph shows mean ± S.D. from one experiment performed in quadruplicate.

    Journal: The Journal of Biological Chemistry

    Article Title: MutT homologue 1 (MTH1) removes N6-methyl-dATP from the dNTP pool

    doi: 10.1074/jbc.RA120.012636

    Figure Lengend Snippet: MTH1 activity with N6-methyl-dATP and N6-methyl-ATP compared with dATP. Activity of 1 and 5 n m MTH1 was tested with 50 μ m N6-methyl-dATP, dATP, ATP, and N6-methyl-ATP in MTH1 reaction buffer (pH 8.0) at 22 °C. Reaction time was 30 min and 0.2 units/ml of PPase was used to generate P i from produced PP i . P i was detected by addition of malachite green reagent followed by measurement of the absorbance at 630 nm. Controls with PPase only was included and background signal was subtracted from the assay data. A P i standard curve was included on the plate enabling determination of the concentration of formed PP i . Graph shows mean ± S.D. from one experiment performed in quadruplicate.

    Article Snippet: MTH1 activity assay Activity of MTH1 (5 or 1 nm ) with 50 μm dATP (Promega), N6-methyl-dATP (Jena Bioscience), ATP (Promega), and N6-methyl-ATP (Jena Bioscience) was assayed in MTH1 reaction buffer (Tris acetate, pH 8.0, 40 mm sodium chloride, 10 mm magnesium acetate).

    Techniques: Activity Assay, Produced, Concentration Assay