dntp mix (Jena Bioscience)

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Jena Bioscience manufactures this product

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Name:

dNTP Mix 10 mM Solution

Description:

Catalog Number:

nu-1006l

Price:

None

Size:

5x 1 ml

Category:

Molecular Biology

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Structured Review

Jena Bioscience dntp mix

https://www.bioz.com/result/dntp mix/product/Jena Bioscience
Average 93 stars, based on 11 article reviews
Price from $9.99 to $1999.99

dntp mix - by Bioz Stars, 2021-01

93/100 stars

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Images

Polymerase Chain Reaction:

Article Title: Construction and Selection of Affilin® Phage Display Libraries.
Article Snippet: .. Affilin < sup > ® < /sup > molecules represent a new class of so-called scaffold proteins. .. Affilin < sup > ® < /sup > molecules represent a new class of so-called scaffold proteins.

Mutagenesis:

Purification:

Article Title: Meglumine Antimoniate and Miltefosine Combined With Allopurinol Sustain Pro-inflammatory Immune Environments During Canine Leishmaniosis Treatment
Article Snippet: .. For cDNA synthesis, 1 μg of purified RNA, presenting a 260/280 absorbance ratio ranging between 1.9 and 2.1 was denatured at 65°C for 5 min and reverse transcribed at 37°C for 60 min in a 30 μl final reaction mixture containing 6 μl of 5× M-MLV RT Buffer (Promega), 200 U/μl SCRIPT Reverse Transcriptase enzyme (Jena Bioscience, Germany), 500 μl dNTP Mix (Jena Bioscience), 1 μl of Oligo(dT)18 primers (Thermo Fisher Scientific Inc.™, EU), and 40 U/μl RiboLock RNase Inhibitor (Thermo Fisher Scientific Inc.™). cDNA samples were then heated at 95°C for 10 min for enzyme inactivation and stored at −20°C until further use. .. Cytokine Gene Expression To evaluate the effect of treatment in pro-inflammatory, anti-inflammatory, and regulatory cytokines, the accumulation of mRNA encoding for IL-2, IL-4, IL-5, IL-10, IL-12, TNF-α, TGF-β, and IFN-γ was assessed by qPCR in PBMC, lymph node and bone marrow cell. cDNA amplification was conducted in a 20 μl final reaction mixture containing 10 μl of SYBR® Green PCR Master Mix (Applied Biosystems™), 80 nM of forward and reverse primers for each cytokine and for housekeeping gene β-actin , 4 μl of ultra-pure water (Merck Millipore™ KGaA) and 2 μl of canine cDNA.