dntp mix  (Jena Bioscience)


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    Name:
    dNTP Mix 10 mM Solution
    Description:
    dNTP Mix is an equimolar mixture of ultrapure dATP dCTP dGTP and dTTP supplied as clear aqueous solution pH 8 5
    Catalog Number:
    nu-1006l
    Price:
    109.12
    Applications:
    For standard PCR applications a final concentration of 200 µM each dNTP is recommended.
    Purity:
    ≥ 99 % (HPLC)
    Category:
    Nucleotides Nucleosides
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    Structured Review

    Jena Bioscience dntp mix
    dNTP Mix is an equimolar mixture of ultrapure dATP dCTP dGTP and dTTP supplied as clear aqueous solution pH 8 5
    https://www.bioz.com/result/dntp mix/product/Jena Bioscience
    Average 93 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    dntp mix - by Bioz Stars, 2020-08
    93/100 stars

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    Related Articles

    Purification:

    Article Title: Meglumine Antimoniate and Miltefosine Combined With Allopurinol Sustain Pro-inflammatory Immune Environments During Canine Leishmaniosis Treatment
    Article Snippet: .. For cDNA synthesis, 1 μg of purified RNA, presenting a 260/280 absorbance ratio ranging between 1.9 and 2.1 was denatured at 65°C for 5 min and reverse transcribed at 37°C for 60 min in a 30 μl final reaction mixture containing 6 μl of 5× M-MLV RT Buffer (Promega), 200 U/μl SCRIPT Reverse Transcriptase enzyme (Jena Bioscience, Germany), 500 μl dNTP Mix (Jena Bioscience), 1 μl of Oligo(dT)18 primers (Thermo Fisher Scientific Inc.™, EU), and 40 U/μl RiboLock RNase Inhibitor (Thermo Fisher Scientific Inc.™). cDNA samples were then heated at 95°C for 10 min for enzyme inactivation and stored at −20°C until further use. .. Cytokine Gene Expression To evaluate the effect of treatment in pro-inflammatory, anti-inflammatory, and regulatory cytokines, the accumulation of mRNA encoding for IL-2, IL-4, IL-5, IL-10, IL-12, TNF-α, TGF-β, and IFN-γ was assessed by qPCR in PBMC, lymph node and bone marrow cell. cDNA amplification was conducted in a 20 μl final reaction mixture containing 10 μl of SYBR® Green PCR Master Mix (Applied Biosystems™), 80 nM of forward and reverse primers for each cytokine and for housekeeping gene β-actin , 4 μl of ultra-pure water (Merck Millipore™ KGaA) and 2 μl of canine cDNA.

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  • 93
    Jena Bioscience dntp mix
    Dntp Mix, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 93/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dntp mix/product/Jena Bioscience
    Average 93 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    dntp mix - by Bioz Stars, 2020-08
    93/100 stars
      Buy from Supplier

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