dgtp  (Jena Bioscience)


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    Name:
    dGTP Solution
    Description:
    dGTP PCR grade is supplied as ultrapure aqueous solution pH 8 5 and suitable for all molecular biology applications including PCR qPCR reverse transcription DNA labeling and DNA sequencing
    Catalog Number:
    nu-1003l
    Price:
    68.2
    Purity:
    ≥ 99 % (HPLC)
    Category:
    Nucleotides Nucleosides
    Buy from Supplier


    Structured Review

    Jena Bioscience dgtp
    Binding of mant-deoxyguanine nucleotides to EFL1. Mant fluorescence was excited by FRET from the intrinsic tryptophan residues in EFL1. Concentrations after mixing consisted of 4 μ m EFL1, 50 μ m <t>mant-deoxy-GDP/deoxy-GTP,</t> and 5 m m Mg 2+ as
    dGTP PCR grade is supplied as ultrapure aqueous solution pH 8 5 and suitable for all molecular biology applications including PCR qPCR reverse transcription DNA labeling and DNA sequencing
    https://www.bioz.com/result/dgtp/product/Jena Bioscience
    Average 93 stars, based on 16 article reviews
    Price from $9.99 to $1999.99
    dgtp - by Bioz Stars, 2020-08
    93/100 stars

    Images

    1) Product Images from "Defective Guanine Nucleotide Exchange in the Elongation Factor-like 1 (EFL1) GTPase by Mutations in the Shwachman-Diamond Syndrome Protein *"

    Article Title: Defective Guanine Nucleotide Exchange in the Elongation Factor-like 1 (EFL1) GTPase by Mutations in the Shwachman-Diamond Syndrome Protein *

    Journal: The Journal of Biological Chemistry

    doi: 10.1074/jbc.M114.626275

    Binding of mant-deoxyguanine nucleotides to EFL1. Mant fluorescence was excited by FRET from the intrinsic tryptophan residues in EFL1. Concentrations after mixing consisted of 4 μ m EFL1, 50 μ m mant-deoxy-GDP/deoxy-GTP, and 5 m m Mg 2+ as
    Figure Legend Snippet: Binding of mant-deoxyguanine nucleotides to EFL1. Mant fluorescence was excited by FRET from the intrinsic tryptophan residues in EFL1. Concentrations after mixing consisted of 4 μ m EFL1, 50 μ m mant-deoxy-GDP/deoxy-GTP, and 5 m m Mg 2+ as

    Techniques Used: Binding Assay, Fluorescence

    Related Articles

    other:

    Article Title: Simple methods for the 3′ biotinylation of RNA
    Article Snippet: The reaction mix contained 0.2 mM dCTP, dATP, dGTP; and 0.5 mM γ-[ N -(biotin-6-amino-hexanoyl)]-5-aminoallyl-dUTP (biotin-11-dUTP; Jena Bioscience).

    Article Title: Defective Guanine Nucleotide Exchange in the Elongation Factor-like 1 (EFL1) GTPase by Mutations in the Shwachman-Diamond Syndrome Protein *
    Article Snippet: The fluorescent mant-derivatives of GDP, GTP, dGDP, dGTP, and GppNHp were obtained from Jena Biosciences (Jena, Germany).

    Nick Translation:

    Article Title: Absence of W Chromosome in Psychidae Moths and Implications for the Theory of Sex Chromosome Evolution in Lepidoptera
    Article Snippet: .. The 20 µL labelling reaction contained 1000 ng of gDNA; 0.05 mM each dATP, dCTP, and dGTP; 0.01 mM dTTP; 0.02 mM labelled nucleotides with either Cy3-dUTP (male gDNA) or fluorescein-dUTP (female gDNA) (both Jena Bioscience, Jena, Germany), nick translation buffer (50 mM TrisHCl, pH 7.5, 5 mM MgCl2 , 0.005% BSA), 10 mM β-mercaptoethanol, 20 U DNA Polymerase I (ThermoFisher Scientific, Waltham, MA, USA) and 0.005 U DNase I (ThermoFisher Scientific). .. The reaction was incubated at 15 °C for 2.5 h. For hybridization mixture per slide, 250 ng of each labelled probe and 25 µg of sonicated salmon sperm DNA (Sigma-Aldrich) were mixed together, precipitated, and re-dissolved in 50% deionized formamide, 10% dextran sulphate, and 2× SSC.

    Article Title: Sex Chromosomes of the Iconic Moth Abraxas grossulariata (Lepidoptera, Geometridae) and Its Congener A. sylvata
    Article Snippet: .. The 25 μL nick translation reaction contained 500 ng gDNA; 25 µM dATP, dCTP, and dGTP; 9 µM dTTP; 16 µM labeled nucleotides with either Cy3-dUTP (male gDNA) or fluorescein-12-dUTP (female gDNA) (both Jena Bioscience, Jena, Germany); 1× nick translation buffer and 5 µL of nick translation enzyme mix. .. The reaction was incubated at 15 °C for 6–7 h. CGH was performed according to a published protocol [ ] with modifications described previously [ ].

    Labeling:

    Article Title: Sex Chromosomes of the Iconic Moth Abraxas grossulariata (Lepidoptera, Geometridae) and Its Congener A. sylvata
    Article Snippet: .. The 25 μL nick translation reaction contained 500 ng gDNA; 25 µM dATP, dCTP, and dGTP; 9 µM dTTP; 16 µM labeled nucleotides with either Cy3-dUTP (male gDNA) or fluorescein-12-dUTP (female gDNA) (both Jena Bioscience, Jena, Germany); 1× nick translation buffer and 5 µL of nick translation enzyme mix. .. The reaction was incubated at 15 °C for 6–7 h. CGH was performed according to a published protocol [ ] with modifications described previously [ ].

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  • 93
    Jena Bioscience dgtp
    Binding of mant-deoxyguanine nucleotides to EFL1. Mant fluorescence was excited by FRET from the intrinsic tryptophan residues in EFL1. Concentrations after mixing consisted of 4 μ m EFL1, 50 μ m <t>mant-deoxy-GDP/deoxy-GTP,</t> and 5 m m Mg 2+ as
    Dgtp, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 93/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dgtp/product/Jena Bioscience
    Average 93 stars, based on 16 article reviews
    Price from $9.99 to $1999.99
    dgtp - by Bioz Stars, 2020-08
    93/100 stars
      Buy from Supplier

    Image Search Results


    Binding of mant-deoxyguanine nucleotides to EFL1. Mant fluorescence was excited by FRET from the intrinsic tryptophan residues in EFL1. Concentrations after mixing consisted of 4 μ m EFL1, 50 μ m mant-deoxy-GDP/deoxy-GTP, and 5 m m Mg 2+ as

    Journal: The Journal of Biological Chemistry

    Article Title: Defective Guanine Nucleotide Exchange in the Elongation Factor-like 1 (EFL1) GTPase by Mutations in the Shwachman-Diamond Syndrome Protein *

    doi: 10.1074/jbc.M114.626275

    Figure Lengend Snippet: Binding of mant-deoxyguanine nucleotides to EFL1. Mant fluorescence was excited by FRET from the intrinsic tryptophan residues in EFL1. Concentrations after mixing consisted of 4 μ m EFL1, 50 μ m mant-deoxy-GDP/deoxy-GTP, and 5 m m Mg 2+ as

    Article Snippet: The fluorescent mant-derivatives of GDP, GTP, dGDP, dGTP, and GppNHp were obtained from Jena Biosciences (Jena, Germany).

    Techniques: Binding Assay, Fluorescence