nthi strains  (ATCC)


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    ATCC nthi strains
    <t>NTHi</t> penetration of bronchial epithelial <t>cells.</t> <t>BEAS-2B</t> cells were infected with one of two NTHi strains (ATCC 19418 or HUSM 0481), E. coli (a negative control), or L. monocytogenes (a positive control) for 2 hours. After killing extracellular bacteria with gentamicin, epithelial cells and bacteria were stained with LIVE/DEAD® and Hoechst. a Representative fluorescence micrographs of L. monocytogenes , and NTHi strain ATCC 19418. White arrows show viable intracellular bacteria stained green. White bars represent 5 μm. Fluorescent micrographs were taken at 2,000× magnification. b The percentages of epithelial cells invaded by bacteria. c After killing extracellular bacteria with gentamicin, cells were lysed and the bacteria were cultured overnight. The number of colonies was counted and the percentages of CFU after gentamicin treatment per input CFU were shown. Error bars represent SEM in three independent experiments that gave similar results. * p < 0.05 and ** p < 0.01
    Nthi Strains, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nthi strains/product/ATCC
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    nthi strains - by Bioz Stars, 2024-02
    99/100 stars

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    1) Product Images from "Nontypeable Haemophilus influenzae exploits the interaction between protein-E and vitronectin for the adherence and invasion to bronchial epithelial cells"

    Article Title: Nontypeable Haemophilus influenzae exploits the interaction between protein-E and vitronectin for the adherence and invasion to bronchial epithelial cells

    Journal: BMC Microbiology

    doi: 10.1186/s12866-015-0600-8

    NTHi penetration of bronchial epithelial cells. BEAS-2B cells were infected with one of two NTHi strains (ATCC 19418 or HUSM 0481), E. coli (a negative control), or L. monocytogenes (a positive control) for 2 hours. After killing extracellular bacteria with gentamicin, epithelial cells and bacteria were stained with LIVE/DEAD® and Hoechst. a Representative fluorescence micrographs of L. monocytogenes , and NTHi strain ATCC 19418. White arrows show viable intracellular bacteria stained green. White bars represent 5 μm. Fluorescent micrographs were taken at 2,000× magnification. b The percentages of epithelial cells invaded by bacteria. c After killing extracellular bacteria with gentamicin, cells were lysed and the bacteria were cultured overnight. The number of colonies was counted and the percentages of CFU after gentamicin treatment per input CFU were shown. Error bars represent SEM in three independent experiments that gave similar results. * p < 0.05 and ** p < 0.01
    Figure Legend Snippet: NTHi penetration of bronchial epithelial cells. BEAS-2B cells were infected with one of two NTHi strains (ATCC 19418 or HUSM 0481), E. coli (a negative control), or L. monocytogenes (a positive control) for 2 hours. After killing extracellular bacteria with gentamicin, epithelial cells and bacteria were stained with LIVE/DEAD® and Hoechst. a Representative fluorescence micrographs of L. monocytogenes , and NTHi strain ATCC 19418. White arrows show viable intracellular bacteria stained green. White bars represent 5 μm. Fluorescent micrographs were taken at 2,000× magnification. b The percentages of epithelial cells invaded by bacteria. c After killing extracellular bacteria with gentamicin, cells were lysed and the bacteria were cultured overnight. The number of colonies was counted and the percentages of CFU after gentamicin treatment per input CFU were shown. Error bars represent SEM in three independent experiments that gave similar results. * p < 0.05 and ** p < 0.01

    Techniques Used: Infection, Negative Control, Positive Control, Staining, Fluorescence, Cell Culture

    Localization of intracellular NTHi. BEAS-2B cells were infected with NTHi strain ATCC 19418 for 2 hours. After killing extracellular bacteria with gentamicin, epithelial cells and bacteria were stained with several fluorescent dyes and Hoechst (blue). a Representative fluorescent micrographs of intracellular NTHi (blue) and epithelial cells stained with early endosomal marker (EEA-1, red). White arrows show EEA-1-positive regions, and white arrowheads show NTHi. For a detail of merged image, see a top-right inset. b Representative fluorescence micrographs of intracellular NTHi (blue) and epithelial cells stained with late endosomal marker (LAMP-1, purple). White arrows show LAMP-1-positive regions, and white arrowheads show NTHi. For a detail of merged image, see a top-right inset. c Representative fluorescent micrographs of intracellular NTHi and epithelial cells stained with LIVE/DEAD® (green) and cells were stained with acidic lysosomal dye (LysoTracker® Red). White arrows show LysoTracker® Red-positive acidic organelles, and white arrowheads show NTHi. Fluorescent micrographs were taken at 2,000× magnification. White bars represent 5 μm. Another strain (HUSM 0481) was also tested and similar results were obtained
    Figure Legend Snippet: Localization of intracellular NTHi. BEAS-2B cells were infected with NTHi strain ATCC 19418 for 2 hours. After killing extracellular bacteria with gentamicin, epithelial cells and bacteria were stained with several fluorescent dyes and Hoechst (blue). a Representative fluorescent micrographs of intracellular NTHi (blue) and epithelial cells stained with early endosomal marker (EEA-1, red). White arrows show EEA-1-positive regions, and white arrowheads show NTHi. For a detail of merged image, see a top-right inset. b Representative fluorescence micrographs of intracellular NTHi (blue) and epithelial cells stained with late endosomal marker (LAMP-1, purple). White arrows show LAMP-1-positive regions, and white arrowheads show NTHi. For a detail of merged image, see a top-right inset. c Representative fluorescent micrographs of intracellular NTHi and epithelial cells stained with LIVE/DEAD® (green) and cells were stained with acidic lysosomal dye (LysoTracker® Red). White arrows show LysoTracker® Red-positive acidic organelles, and white arrowheads show NTHi. Fluorescent micrographs were taken at 2,000× magnification. White bars represent 5 μm. Another strain (HUSM 0481) was also tested and similar results were obtained

    Techniques Used: Infection, Staining, Marker, Fluorescence

    NTHi binding of immobilized vitronectin in the absence or presence of heparin. Human plasma vitronectin was bound to glass-bottomed dishes. NTHi was incubated on the plate-bound vitronectin for 30 minutes, and then the dishes were washed three times. In some experiments, plate-bound vitronectin was pretreated with heparin before incubation with NTHi. NTHi was stained with LIVE/DEAD®. Live NTHi is stained green. BSA was used as a negative control. Representative fluorescent micrographs show that both NTHi strains (ATCC 19418 ( a ) and HUSM 0481 ( b )) attached to plate-bound vitronectin and that this attachment is blocked by heparin. White bars represent 10 μm. Summaries of the numbers of attached NTHi (per field at 1,000× magnification) are shown in ( c ) for ATCC 19418 and ( d ) for HUSM 0481. Error bars represent SEM in three independent experiments that gave similar results. ** p < 0.01
    Figure Legend Snippet: NTHi binding of immobilized vitronectin in the absence or presence of heparin. Human plasma vitronectin was bound to glass-bottomed dishes. NTHi was incubated on the plate-bound vitronectin for 30 minutes, and then the dishes were washed three times. In some experiments, plate-bound vitronectin was pretreated with heparin before incubation with NTHi. NTHi was stained with LIVE/DEAD®. Live NTHi is stained green. BSA was used as a negative control. Representative fluorescent micrographs show that both NTHi strains (ATCC 19418 ( a ) and HUSM 0481 ( b )) attached to plate-bound vitronectin and that this attachment is blocked by heparin. White bars represent 10 μm. Summaries of the numbers of attached NTHi (per field at 1,000× magnification) are shown in ( c ) for ATCC 19418 and ( d ) for HUSM 0481. Error bars represent SEM in three independent experiments that gave similar results. ** p < 0.01

    Techniques Used: Binding Assay, Incubation, Staining, Negative Control

    Intracellular invasion of NTHi in the presence of heparin or RGD peptide. BEAS-2B cells were infected for 2 hours with one of the two NTHi strains (ATCC 19418 or HUSM 0481), E. coli (a negative control), or L. monocytogenes (a positive control). In some experiments, cells were pretreated with heparin or RGD-peptide. a After killing extracellular bacteria with gentamicin, epithelial cells and bacteria were stained with LIVE/DEAD®. The percentages of BEAS-2B cells invaded by each type of bacteria are shown. b After killing extracellular bacteria with gentamicin and lysing the BEAS-2B cells, the bacteria were cultured overnight. The number of colonies was counted and the percentages of CFU after gentamicin treatment of cells per input CFU were shown. Error bars represent SEM in three independent experiments that gave similar results. * p < 0.05 and ** p < 0.01
    Figure Legend Snippet: Intracellular invasion of NTHi in the presence of heparin or RGD peptide. BEAS-2B cells were infected for 2 hours with one of the two NTHi strains (ATCC 19418 or HUSM 0481), E. coli (a negative control), or L. monocytogenes (a positive control). In some experiments, cells were pretreated with heparin or RGD-peptide. a After killing extracellular bacteria with gentamicin, epithelial cells and bacteria were stained with LIVE/DEAD®. The percentages of BEAS-2B cells invaded by each type of bacteria are shown. b After killing extracellular bacteria with gentamicin and lysing the BEAS-2B cells, the bacteria were cultured overnight. The number of colonies was counted and the percentages of CFU after gentamicin treatment of cells per input CFU were shown. Error bars represent SEM in three independent experiments that gave similar results. * p < 0.05 and ** p < 0.01

    Techniques Used: Infection, Negative Control, Positive Control, Staining, Cell Culture

    Binding of NTHi to immobilized vitronectin in the presence of protein-E peptide. Human plasma vitronectin was bound to glass-bottomed dishes. NTHi (HUSM 0481) was incubated on the plate-bound vitronectin for 30 minutes, and then the dishes were washed three times. In some experiments, plate-bound vitronectin was pretreated with protein-E peptide (PE 84–108 ) before incubation with NTHi. BSA was used as a negative control. NTHi was stained with LIVE/DEAD®, and viable NTHi is stained green. a Representative fluorescent micrographs of NTHi incubated on plate-bound vitronectin that was untreated or pretreated with PE 84–108 . White bars represent 10 μm. b The number of attached NTHi per field at 1,000 × magnification that was pretreated with increasing concentrations of PE 84–108 . Error bars represent SEM in three independent experiments that gave similar results. ** p < 0.01
    Figure Legend Snippet: Binding of NTHi to immobilized vitronectin in the presence of protein-E peptide. Human plasma vitronectin was bound to glass-bottomed dishes. NTHi (HUSM 0481) was incubated on the plate-bound vitronectin for 30 minutes, and then the dishes were washed three times. In some experiments, plate-bound vitronectin was pretreated with protein-E peptide (PE 84–108 ) before incubation with NTHi. BSA was used as a negative control. NTHi was stained with LIVE/DEAD®, and viable NTHi is stained green. a Representative fluorescent micrographs of NTHi incubated on plate-bound vitronectin that was untreated or pretreated with PE 84–108 . White bars represent 10 μm. b The number of attached NTHi per field at 1,000 × magnification that was pretreated with increasing concentrations of PE 84–108 . Error bars represent SEM in three independent experiments that gave similar results. ** p < 0.01

    Techniques Used: Binding Assay, Incubation, Negative Control, Staining

    Intracellular invasion of NTHi in the absence or presence of protein-E. BEAS-2B cells were infected for 2 hours with one of the two NTHi strains (ATCC 19418 or HUSM 0481) with or without pretreatment with PE 84–108 peptide. a After killing extracellular bacteria with gentamicin, epithelial cells and bacteria were stained with LIVE/DEAD®. The percentages of BEAS-2B cells invaded by each NTHi strain are shown. b After killing extracellular bacteria with gentamicin, cells were lysed and the bacteria were cultured overnight. The number of colonies was counted and the percentages of CFU after gentamicin treatment of cells per input CFU were shown. Error bars represent SEM in three independent experiments that gave similar results. * p < 0.05 and ** p < 0.01
    Figure Legend Snippet: Intracellular invasion of NTHi in the absence or presence of protein-E. BEAS-2B cells were infected for 2 hours with one of the two NTHi strains (ATCC 19418 or HUSM 0481) with or without pretreatment with PE 84–108 peptide. a After killing extracellular bacteria with gentamicin, epithelial cells and bacteria were stained with LIVE/DEAD®. The percentages of BEAS-2B cells invaded by each NTHi strain are shown. b After killing extracellular bacteria with gentamicin, cells were lysed and the bacteria were cultured overnight. The number of colonies was counted and the percentages of CFU after gentamicin treatment of cells per input CFU were shown. Error bars represent SEM in three independent experiments that gave similar results. * p < 0.05 and ** p < 0.01

    Techniques Used: Infection, Staining, Cell Culture

    nthi 19418  (ATCC)


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    ATCC nthi 19418
    Nthi 19418, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    nthi 19418  (ATCC)


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    Structured Review

    ATCC nthi 19418
    Nthi 19418, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    nthi strains  (ATCC)


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    Structured Review

    ATCC nthi strains
    <t>NTHi</t> penetration of bronchial epithelial <t>cells.</t> <t>BEAS-2B</t> cells were infected with one of two NTHi strains (ATCC 19418 or HUSM 0481), E. coli (a negative control), or L. monocytogenes (a positive control) for 2 hours. After killing extracellular bacteria with gentamicin, epithelial cells and bacteria were stained with LIVE/DEAD® and Hoechst. a Representative fluorescence micrographs of L. monocytogenes , and NTHi strain ATCC 19418. White arrows show viable intracellular bacteria stained green. White bars represent 5 μm. Fluorescent micrographs were taken at 2,000× magnification. b The percentages of epithelial cells invaded by bacteria. c After killing extracellular bacteria with gentamicin, cells were lysed and the bacteria were cultured overnight. The number of colonies was counted and the percentages of CFU after gentamicin treatment per input CFU were shown. Error bars represent SEM in three independent experiments that gave similar results. * p < 0.05 and ** p < 0.01
    Nthi Strains, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nthi strains/product/ATCC
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    nthi strains - by Bioz Stars, 2024-02
    99/100 stars

    Images

    1) Product Images from "Nontypeable Haemophilus influenzae exploits the interaction between protein-E and vitronectin for the adherence and invasion to bronchial epithelial cells"

    Article Title: Nontypeable Haemophilus influenzae exploits the interaction between protein-E and vitronectin for the adherence and invasion to bronchial epithelial cells

    Journal: BMC Microbiology

    doi: 10.1186/s12866-015-0600-8

    NTHi penetration of bronchial epithelial cells. BEAS-2B cells were infected with one of two NTHi strains (ATCC 19418 or HUSM 0481), E. coli (a negative control), or L. monocytogenes (a positive control) for 2 hours. After killing extracellular bacteria with gentamicin, epithelial cells and bacteria were stained with LIVE/DEAD® and Hoechst. a Representative fluorescence micrographs of L. monocytogenes , and NTHi strain ATCC 19418. White arrows show viable intracellular bacteria stained green. White bars represent 5 μm. Fluorescent micrographs were taken at 2,000× magnification. b The percentages of epithelial cells invaded by bacteria. c After killing extracellular bacteria with gentamicin, cells were lysed and the bacteria were cultured overnight. The number of colonies was counted and the percentages of CFU after gentamicin treatment per input CFU were shown. Error bars represent SEM in three independent experiments that gave similar results. * p < 0.05 and ** p < 0.01
    Figure Legend Snippet: NTHi penetration of bronchial epithelial cells. BEAS-2B cells were infected with one of two NTHi strains (ATCC 19418 or HUSM 0481), E. coli (a negative control), or L. monocytogenes (a positive control) for 2 hours. After killing extracellular bacteria with gentamicin, epithelial cells and bacteria were stained with LIVE/DEAD® and Hoechst. a Representative fluorescence micrographs of L. monocytogenes , and NTHi strain ATCC 19418. White arrows show viable intracellular bacteria stained green. White bars represent 5 μm. Fluorescent micrographs were taken at 2,000× magnification. b The percentages of epithelial cells invaded by bacteria. c After killing extracellular bacteria with gentamicin, cells were lysed and the bacteria were cultured overnight. The number of colonies was counted and the percentages of CFU after gentamicin treatment per input CFU were shown. Error bars represent SEM in three independent experiments that gave similar results. * p < 0.05 and ** p < 0.01

    Techniques Used: Infection, Negative Control, Positive Control, Staining, Fluorescence, Cell Culture

    Localization of intracellular NTHi. BEAS-2B cells were infected with NTHi strain ATCC 19418 for 2 hours. After killing extracellular bacteria with gentamicin, epithelial cells and bacteria were stained with several fluorescent dyes and Hoechst (blue). a Representative fluorescent micrographs of intracellular NTHi (blue) and epithelial cells stained with early endosomal marker (EEA-1, red). White arrows show EEA-1-positive regions, and white arrowheads show NTHi. For a detail of merged image, see a top-right inset. b Representative fluorescence micrographs of intracellular NTHi (blue) and epithelial cells stained with late endosomal marker (LAMP-1, purple). White arrows show LAMP-1-positive regions, and white arrowheads show NTHi. For a detail of merged image, see a top-right inset. c Representative fluorescent micrographs of intracellular NTHi and epithelial cells stained with LIVE/DEAD® (green) and cells were stained with acidic lysosomal dye (LysoTracker® Red). White arrows show LysoTracker® Red-positive acidic organelles, and white arrowheads show NTHi. Fluorescent micrographs were taken at 2,000× magnification. White bars represent 5 μm. Another strain (HUSM 0481) was also tested and similar results were obtained
    Figure Legend Snippet: Localization of intracellular NTHi. BEAS-2B cells were infected with NTHi strain ATCC 19418 for 2 hours. After killing extracellular bacteria with gentamicin, epithelial cells and bacteria were stained with several fluorescent dyes and Hoechst (blue). a Representative fluorescent micrographs of intracellular NTHi (blue) and epithelial cells stained with early endosomal marker (EEA-1, red). White arrows show EEA-1-positive regions, and white arrowheads show NTHi. For a detail of merged image, see a top-right inset. b Representative fluorescence micrographs of intracellular NTHi (blue) and epithelial cells stained with late endosomal marker (LAMP-1, purple). White arrows show LAMP-1-positive regions, and white arrowheads show NTHi. For a detail of merged image, see a top-right inset. c Representative fluorescent micrographs of intracellular NTHi and epithelial cells stained with LIVE/DEAD® (green) and cells were stained with acidic lysosomal dye (LysoTracker® Red). White arrows show LysoTracker® Red-positive acidic organelles, and white arrowheads show NTHi. Fluorescent micrographs were taken at 2,000× magnification. White bars represent 5 μm. Another strain (HUSM 0481) was also tested and similar results were obtained

    Techniques Used: Infection, Staining, Marker, Fluorescence

    NTHi binding of immobilized vitronectin in the absence or presence of heparin. Human plasma vitronectin was bound to glass-bottomed dishes. NTHi was incubated on the plate-bound vitronectin for 30 minutes, and then the dishes were washed three times. In some experiments, plate-bound vitronectin was pretreated with heparin before incubation with NTHi. NTHi was stained with LIVE/DEAD®. Live NTHi is stained green. BSA was used as a negative control. Representative fluorescent micrographs show that both NTHi strains (ATCC 19418 ( a ) and HUSM 0481 ( b )) attached to plate-bound vitronectin and that this attachment is blocked by heparin. White bars represent 10 μm. Summaries of the numbers of attached NTHi (per field at 1,000× magnification) are shown in ( c ) for ATCC 19418 and ( d ) for HUSM 0481. Error bars represent SEM in three independent experiments that gave similar results. ** p < 0.01
    Figure Legend Snippet: NTHi binding of immobilized vitronectin in the absence or presence of heparin. Human plasma vitronectin was bound to glass-bottomed dishes. NTHi was incubated on the plate-bound vitronectin for 30 minutes, and then the dishes were washed three times. In some experiments, plate-bound vitronectin was pretreated with heparin before incubation with NTHi. NTHi was stained with LIVE/DEAD®. Live NTHi is stained green. BSA was used as a negative control. Representative fluorescent micrographs show that both NTHi strains (ATCC 19418 ( a ) and HUSM 0481 ( b )) attached to plate-bound vitronectin and that this attachment is blocked by heparin. White bars represent 10 μm. Summaries of the numbers of attached NTHi (per field at 1,000× magnification) are shown in ( c ) for ATCC 19418 and ( d ) for HUSM 0481. Error bars represent SEM in three independent experiments that gave similar results. ** p < 0.01

    Techniques Used: Binding Assay, Incubation, Staining, Negative Control

    Intracellular invasion of NTHi in the presence of heparin or RGD peptide. BEAS-2B cells were infected for 2 hours with one of the two NTHi strains (ATCC 19418 or HUSM 0481), E. coli (a negative control), or L. monocytogenes (a positive control). In some experiments, cells were pretreated with heparin or RGD-peptide. a After killing extracellular bacteria with gentamicin, epithelial cells and bacteria were stained with LIVE/DEAD®. The percentages of BEAS-2B cells invaded by each type of bacteria are shown. b After killing extracellular bacteria with gentamicin and lysing the BEAS-2B cells, the bacteria were cultured overnight. The number of colonies was counted and the percentages of CFU after gentamicin treatment of cells per input CFU were shown. Error bars represent SEM in three independent experiments that gave similar results. * p < 0.05 and ** p < 0.01
    Figure Legend Snippet: Intracellular invasion of NTHi in the presence of heparin or RGD peptide. BEAS-2B cells were infected for 2 hours with one of the two NTHi strains (ATCC 19418 or HUSM 0481), E. coli (a negative control), or L. monocytogenes (a positive control). In some experiments, cells were pretreated with heparin or RGD-peptide. a After killing extracellular bacteria with gentamicin, epithelial cells and bacteria were stained with LIVE/DEAD®. The percentages of BEAS-2B cells invaded by each type of bacteria are shown. b After killing extracellular bacteria with gentamicin and lysing the BEAS-2B cells, the bacteria were cultured overnight. The number of colonies was counted and the percentages of CFU after gentamicin treatment of cells per input CFU were shown. Error bars represent SEM in three independent experiments that gave similar results. * p < 0.05 and ** p < 0.01

    Techniques Used: Infection, Negative Control, Positive Control, Staining, Cell Culture

    Binding of NTHi to immobilized vitronectin in the presence of protein-E peptide. Human plasma vitronectin was bound to glass-bottomed dishes. NTHi (HUSM 0481) was incubated on the plate-bound vitronectin for 30 minutes, and then the dishes were washed three times. In some experiments, plate-bound vitronectin was pretreated with protein-E peptide (PE 84–108 ) before incubation with NTHi. BSA was used as a negative control. NTHi was stained with LIVE/DEAD®, and viable NTHi is stained green. a Representative fluorescent micrographs of NTHi incubated on plate-bound vitronectin that was untreated or pretreated with PE 84–108 . White bars represent 10 μm. b The number of attached NTHi per field at 1,000 × magnification that was pretreated with increasing concentrations of PE 84–108 . Error bars represent SEM in three independent experiments that gave similar results. ** p < 0.01
    Figure Legend Snippet: Binding of NTHi to immobilized vitronectin in the presence of protein-E peptide. Human plasma vitronectin was bound to glass-bottomed dishes. NTHi (HUSM 0481) was incubated on the plate-bound vitronectin for 30 minutes, and then the dishes were washed three times. In some experiments, plate-bound vitronectin was pretreated with protein-E peptide (PE 84–108 ) before incubation with NTHi. BSA was used as a negative control. NTHi was stained with LIVE/DEAD®, and viable NTHi is stained green. a Representative fluorescent micrographs of NTHi incubated on plate-bound vitronectin that was untreated or pretreated with PE 84–108 . White bars represent 10 μm. b The number of attached NTHi per field at 1,000 × magnification that was pretreated with increasing concentrations of PE 84–108 . Error bars represent SEM in three independent experiments that gave similar results. ** p < 0.01

    Techniques Used: Binding Assay, Incubation, Negative Control, Staining

    Intracellular invasion of NTHi in the absence or presence of protein-E. BEAS-2B cells were infected for 2 hours with one of the two NTHi strains (ATCC 19418 or HUSM 0481) with or without pretreatment with PE 84–108 peptide. a After killing extracellular bacteria with gentamicin, epithelial cells and bacteria were stained with LIVE/DEAD®. The percentages of BEAS-2B cells invaded by each NTHi strain are shown. b After killing extracellular bacteria with gentamicin, cells were lysed and the bacteria were cultured overnight. The number of colonies was counted and the percentages of CFU after gentamicin treatment of cells per input CFU were shown. Error bars represent SEM in three independent experiments that gave similar results. * p < 0.05 and ** p < 0.01
    Figure Legend Snippet: Intracellular invasion of NTHi in the absence or presence of protein-E. BEAS-2B cells were infected for 2 hours with one of the two NTHi strains (ATCC 19418 or HUSM 0481) with or without pretreatment with PE 84–108 peptide. a After killing extracellular bacteria with gentamicin, epithelial cells and bacteria were stained with LIVE/DEAD®. The percentages of BEAS-2B cells invaded by each NTHi strain are shown. b After killing extracellular bacteria with gentamicin, cells were lysed and the bacteria were cultured overnight. The number of colonies was counted and the percentages of CFU after gentamicin treatment of cells per input CFU were shown. Error bars represent SEM in three independent experiments that gave similar results. * p < 0.05 and ** p < 0.01

    Techniques Used: Infection, Staining, Cell Culture

    nthi strains  (ATCC)


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  • 99

    Structured Review

    ATCC nthi strains
    <t>NTHi</t> penetration of bronchial epithelial <t>cells.</t> <t>BEAS-2B</t> cells were infected with one of two NTHi strains (ATCC 19418 or HUSM 0481), E. coli (a negative control), or L. monocytogenes (a positive control) for 2 hours. After killing extracellular bacteria with gentamicin, epithelial cells and bacteria were stained with LIVE/DEAD® and Hoechst. a Representative fluorescence micrographs of L. monocytogenes , and NTHi strain ATCC 19418. White arrows show viable intracellular bacteria stained green. White bars represent 5 μm. Fluorescent micrographs were taken at 2,000× magnification. b The percentages of epithelial cells invaded by bacteria. c After killing extracellular bacteria with gentamicin, cells were lysed and the bacteria were cultured overnight. The number of colonies was counted and the percentages of CFU after gentamicin treatment per input CFU were shown. Error bars represent SEM in three independent experiments that gave similar results. * p < 0.05 and ** p < 0.01
    Nthi Strains, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nthi strains/product/ATCC
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    nthi strains - by Bioz Stars, 2024-02
    99/100 stars

    Images

    1) Product Images from "Nontypeable Haemophilus influenzae exploits the interaction between protein-E and vitronectin for the adherence and invasion to bronchial epithelial cells"

    Article Title: Nontypeable Haemophilus influenzae exploits the interaction between protein-E and vitronectin for the adherence and invasion to bronchial epithelial cells

    Journal: BMC Microbiology

    doi: 10.1186/s12866-015-0600-8

    NTHi penetration of bronchial epithelial cells. BEAS-2B cells were infected with one of two NTHi strains (ATCC 19418 or HUSM 0481), E. coli (a negative control), or L. monocytogenes (a positive control) for 2 hours. After killing extracellular bacteria with gentamicin, epithelial cells and bacteria were stained with LIVE/DEAD® and Hoechst. a Representative fluorescence micrographs of L. monocytogenes , and NTHi strain ATCC 19418. White arrows show viable intracellular bacteria stained green. White bars represent 5 μm. Fluorescent micrographs were taken at 2,000× magnification. b The percentages of epithelial cells invaded by bacteria. c After killing extracellular bacteria with gentamicin, cells were lysed and the bacteria were cultured overnight. The number of colonies was counted and the percentages of CFU after gentamicin treatment per input CFU were shown. Error bars represent SEM in three independent experiments that gave similar results. * p < 0.05 and ** p < 0.01
    Figure Legend Snippet: NTHi penetration of bronchial epithelial cells. BEAS-2B cells were infected with one of two NTHi strains (ATCC 19418 or HUSM 0481), E. coli (a negative control), or L. monocytogenes (a positive control) for 2 hours. After killing extracellular bacteria with gentamicin, epithelial cells and bacteria were stained with LIVE/DEAD® and Hoechst. a Representative fluorescence micrographs of L. monocytogenes , and NTHi strain ATCC 19418. White arrows show viable intracellular bacteria stained green. White bars represent 5 μm. Fluorescent micrographs were taken at 2,000× magnification. b The percentages of epithelial cells invaded by bacteria. c After killing extracellular bacteria with gentamicin, cells were lysed and the bacteria were cultured overnight. The number of colonies was counted and the percentages of CFU after gentamicin treatment per input CFU were shown. Error bars represent SEM in three independent experiments that gave similar results. * p < 0.05 and ** p < 0.01

    Techniques Used: Infection, Negative Control, Positive Control, Staining, Fluorescence, Cell Culture

    Localization of intracellular NTHi. BEAS-2B cells were infected with NTHi strain ATCC 19418 for 2 hours. After killing extracellular bacteria with gentamicin, epithelial cells and bacteria were stained with several fluorescent dyes and Hoechst (blue). a Representative fluorescent micrographs of intracellular NTHi (blue) and epithelial cells stained with early endosomal marker (EEA-1, red). White arrows show EEA-1-positive regions, and white arrowheads show NTHi. For a detail of merged image, see a top-right inset. b Representative fluorescence micrographs of intracellular NTHi (blue) and epithelial cells stained with late endosomal marker (LAMP-1, purple). White arrows show LAMP-1-positive regions, and white arrowheads show NTHi. For a detail of merged image, see a top-right inset. c Representative fluorescent micrographs of intracellular NTHi and epithelial cells stained with LIVE/DEAD® (green) and cells were stained with acidic lysosomal dye (LysoTracker® Red). White arrows show LysoTracker® Red-positive acidic organelles, and white arrowheads show NTHi. Fluorescent micrographs were taken at 2,000× magnification. White bars represent 5 μm. Another strain (HUSM 0481) was also tested and similar results were obtained
    Figure Legend Snippet: Localization of intracellular NTHi. BEAS-2B cells were infected with NTHi strain ATCC 19418 for 2 hours. After killing extracellular bacteria with gentamicin, epithelial cells and bacteria were stained with several fluorescent dyes and Hoechst (blue). a Representative fluorescent micrographs of intracellular NTHi (blue) and epithelial cells stained with early endosomal marker (EEA-1, red). White arrows show EEA-1-positive regions, and white arrowheads show NTHi. For a detail of merged image, see a top-right inset. b Representative fluorescence micrographs of intracellular NTHi (blue) and epithelial cells stained with late endosomal marker (LAMP-1, purple). White arrows show LAMP-1-positive regions, and white arrowheads show NTHi. For a detail of merged image, see a top-right inset. c Representative fluorescent micrographs of intracellular NTHi and epithelial cells stained with LIVE/DEAD® (green) and cells were stained with acidic lysosomal dye (LysoTracker® Red). White arrows show LysoTracker® Red-positive acidic organelles, and white arrowheads show NTHi. Fluorescent micrographs were taken at 2,000× magnification. White bars represent 5 μm. Another strain (HUSM 0481) was also tested and similar results were obtained

    Techniques Used: Infection, Staining, Marker, Fluorescence

    NTHi binding of immobilized vitronectin in the absence or presence of heparin. Human plasma vitronectin was bound to glass-bottomed dishes. NTHi was incubated on the plate-bound vitronectin for 30 minutes, and then the dishes were washed three times. In some experiments, plate-bound vitronectin was pretreated with heparin before incubation with NTHi. NTHi was stained with LIVE/DEAD®. Live NTHi is stained green. BSA was used as a negative control. Representative fluorescent micrographs show that both NTHi strains (ATCC 19418 ( a ) and HUSM 0481 ( b )) attached to plate-bound vitronectin and that this attachment is blocked by heparin. White bars represent 10 μm. Summaries of the numbers of attached NTHi (per field at 1,000× magnification) are shown in ( c ) for ATCC 19418 and ( d ) for HUSM 0481. Error bars represent SEM in three independent experiments that gave similar results. ** p < 0.01
    Figure Legend Snippet: NTHi binding of immobilized vitronectin in the absence or presence of heparin. Human plasma vitronectin was bound to glass-bottomed dishes. NTHi was incubated on the plate-bound vitronectin for 30 minutes, and then the dishes were washed three times. In some experiments, plate-bound vitronectin was pretreated with heparin before incubation with NTHi. NTHi was stained with LIVE/DEAD®. Live NTHi is stained green. BSA was used as a negative control. Representative fluorescent micrographs show that both NTHi strains (ATCC 19418 ( a ) and HUSM 0481 ( b )) attached to plate-bound vitronectin and that this attachment is blocked by heparin. White bars represent 10 μm. Summaries of the numbers of attached NTHi (per field at 1,000× magnification) are shown in ( c ) for ATCC 19418 and ( d ) for HUSM 0481. Error bars represent SEM in three independent experiments that gave similar results. ** p < 0.01

    Techniques Used: Binding Assay, Incubation, Staining, Negative Control

    Intracellular invasion of NTHi in the presence of heparin or RGD peptide. BEAS-2B cells were infected for 2 hours with one of the two NTHi strains (ATCC 19418 or HUSM 0481), E. coli (a negative control), or L. monocytogenes (a positive control). In some experiments, cells were pretreated with heparin or RGD-peptide. a After killing extracellular bacteria with gentamicin, epithelial cells and bacteria were stained with LIVE/DEAD®. The percentages of BEAS-2B cells invaded by each type of bacteria are shown. b After killing extracellular bacteria with gentamicin and lysing the BEAS-2B cells, the bacteria were cultured overnight. The number of colonies was counted and the percentages of CFU after gentamicin treatment of cells per input CFU were shown. Error bars represent SEM in three independent experiments that gave similar results. * p < 0.05 and ** p < 0.01
    Figure Legend Snippet: Intracellular invasion of NTHi in the presence of heparin or RGD peptide. BEAS-2B cells were infected for 2 hours with one of the two NTHi strains (ATCC 19418 or HUSM 0481), E. coli (a negative control), or L. monocytogenes (a positive control). In some experiments, cells were pretreated with heparin or RGD-peptide. a After killing extracellular bacteria with gentamicin, epithelial cells and bacteria were stained with LIVE/DEAD®. The percentages of BEAS-2B cells invaded by each type of bacteria are shown. b After killing extracellular bacteria with gentamicin and lysing the BEAS-2B cells, the bacteria were cultured overnight. The number of colonies was counted and the percentages of CFU after gentamicin treatment of cells per input CFU were shown. Error bars represent SEM in three independent experiments that gave similar results. * p < 0.05 and ** p < 0.01

    Techniques Used: Infection, Negative Control, Positive Control, Staining, Cell Culture

    Binding of NTHi to immobilized vitronectin in the presence of protein-E peptide. Human plasma vitronectin was bound to glass-bottomed dishes. NTHi (HUSM 0481) was incubated on the plate-bound vitronectin for 30 minutes, and then the dishes were washed three times. In some experiments, plate-bound vitronectin was pretreated with protein-E peptide (PE 84–108 ) before incubation with NTHi. BSA was used as a negative control. NTHi was stained with LIVE/DEAD®, and viable NTHi is stained green. a Representative fluorescent micrographs of NTHi incubated on plate-bound vitronectin that was untreated or pretreated with PE 84–108 . White bars represent 10 μm. b The number of attached NTHi per field at 1,000 × magnification that was pretreated with increasing concentrations of PE 84–108 . Error bars represent SEM in three independent experiments that gave similar results. ** p < 0.01
    Figure Legend Snippet: Binding of NTHi to immobilized vitronectin in the presence of protein-E peptide. Human plasma vitronectin was bound to glass-bottomed dishes. NTHi (HUSM 0481) was incubated on the plate-bound vitronectin for 30 minutes, and then the dishes were washed three times. In some experiments, plate-bound vitronectin was pretreated with protein-E peptide (PE 84–108 ) before incubation with NTHi. BSA was used as a negative control. NTHi was stained with LIVE/DEAD®, and viable NTHi is stained green. a Representative fluorescent micrographs of NTHi incubated on plate-bound vitronectin that was untreated or pretreated with PE 84–108 . White bars represent 10 μm. b The number of attached NTHi per field at 1,000 × magnification that was pretreated with increasing concentrations of PE 84–108 . Error bars represent SEM in three independent experiments that gave similar results. ** p < 0.01

    Techniques Used: Binding Assay, Incubation, Negative Control, Staining

    Intracellular invasion of NTHi in the absence or presence of protein-E. BEAS-2B cells were infected for 2 hours with one of the two NTHi strains (ATCC 19418 or HUSM 0481) with or without pretreatment with PE 84–108 peptide. a After killing extracellular bacteria with gentamicin, epithelial cells and bacteria were stained with LIVE/DEAD®. The percentages of BEAS-2B cells invaded by each NTHi strain are shown. b After killing extracellular bacteria with gentamicin, cells were lysed and the bacteria were cultured overnight. The number of colonies was counted and the percentages of CFU after gentamicin treatment of cells per input CFU were shown. Error bars represent SEM in three independent experiments that gave similar results. * p < 0.05 and ** p < 0.01
    Figure Legend Snippet: Intracellular invasion of NTHi in the absence or presence of protein-E. BEAS-2B cells were infected for 2 hours with one of the two NTHi strains (ATCC 19418 or HUSM 0481) with or without pretreatment with PE 84–108 peptide. a After killing extracellular bacteria with gentamicin, epithelial cells and bacteria were stained with LIVE/DEAD®. The percentages of BEAS-2B cells invaded by each NTHi strain are shown. b After killing extracellular bacteria with gentamicin, cells were lysed and the bacteria were cultured overnight. The number of colonies was counted and the percentages of CFU after gentamicin treatment of cells per input CFU were shown. Error bars represent SEM in three independent experiments that gave similar results. * p < 0.05 and ** p < 0.01

    Techniques Used: Infection, Staining, Cell Culture

    nthi strains a atcc 19418  (ATCC)


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    ATCC nthi strains a atcc 19418
    NTHi penetration of bronchial epithelial cells. BEAS-2B cells were infected with one of two NTHi strains (ATCC 19418 or HUSM 0481), E. coli (a negative control), or L. monocytogenes (a positive control) for 2 hours. After killing extracellular bacteria with gentamicin, epithelial cells and bacteria were stained with LIVE/DEAD® and Hoechst. a Representative fluorescence micrographs of L. monocytogenes , and NTHi strain ATCC 19418. White arrows show viable intracellular bacteria stained green. White bars represent 5 μm. Fluorescent micrographs were taken at 2,000× magnification. b The percentages of epithelial cells invaded by bacteria. c After killing extracellular bacteria with gentamicin, cells were lysed and the bacteria were cultured overnight. The number of colonies was counted and the percentages of CFU after gentamicin treatment per input CFU were shown. Error bars represent SEM in three independent experiments that gave similar results. * p < 0.05 and ** p < 0.01
    Nthi Strains A Atcc 19418, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Nontypeable Haemophilus influenzae exploits the interaction between protein-E and vitronectin for the adherence and invasion to bronchial epithelial cells"

    Article Title: Nontypeable Haemophilus influenzae exploits the interaction between protein-E and vitronectin for the adherence and invasion to bronchial epithelial cells

    Journal: BMC Microbiology

    doi: 10.1186/s12866-015-0600-8

    NTHi penetration of bronchial epithelial cells. BEAS-2B cells were infected with one of two NTHi strains (ATCC 19418 or HUSM 0481), E. coli (a negative control), or L. monocytogenes (a positive control) for 2 hours. After killing extracellular bacteria with gentamicin, epithelial cells and bacteria were stained with LIVE/DEAD® and Hoechst. a Representative fluorescence micrographs of L. monocytogenes , and NTHi strain ATCC 19418. White arrows show viable intracellular bacteria stained green. White bars represent 5 μm. Fluorescent micrographs were taken at 2,000× magnification. b The percentages of epithelial cells invaded by bacteria. c After killing extracellular bacteria with gentamicin, cells were lysed and the bacteria were cultured overnight. The number of colonies was counted and the percentages of CFU after gentamicin treatment per input CFU were shown. Error bars represent SEM in three independent experiments that gave similar results. * p < 0.05 and ** p < 0.01
    Figure Legend Snippet: NTHi penetration of bronchial epithelial cells. BEAS-2B cells were infected with one of two NTHi strains (ATCC 19418 or HUSM 0481), E. coli (a negative control), or L. monocytogenes (a positive control) for 2 hours. After killing extracellular bacteria with gentamicin, epithelial cells and bacteria were stained with LIVE/DEAD® and Hoechst. a Representative fluorescence micrographs of L. monocytogenes , and NTHi strain ATCC 19418. White arrows show viable intracellular bacteria stained green. White bars represent 5 μm. Fluorescent micrographs were taken at 2,000× magnification. b The percentages of epithelial cells invaded by bacteria. c After killing extracellular bacteria with gentamicin, cells were lysed and the bacteria were cultured overnight. The number of colonies was counted and the percentages of CFU after gentamicin treatment per input CFU were shown. Error bars represent SEM in three independent experiments that gave similar results. * p < 0.05 and ** p < 0.01

    Techniques Used: Infection, Negative Control, Positive Control, Staining, Fluorescence, Cell Culture

    Localization of intracellular NTHi. BEAS-2B cells were infected with NTHi strain ATCC 19418 for 2 hours. After killing extracellular bacteria with gentamicin, epithelial cells and bacteria were stained with several fluorescent dyes and Hoechst (blue). a Representative fluorescent micrographs of intracellular NTHi (blue) and epithelial cells stained with early endosomal marker (EEA-1, red). White arrows show EEA-1-positive regions, and white arrowheads show NTHi. For a detail of merged image, see a top-right inset. b Representative fluorescence micrographs of intracellular NTHi (blue) and epithelial cells stained with late endosomal marker (LAMP-1, purple). White arrows show LAMP-1-positive regions, and white arrowheads show NTHi. For a detail of merged image, see a top-right inset. c Representative fluorescent micrographs of intracellular NTHi and epithelial cells stained with LIVE/DEAD® (green) and cells were stained with acidic lysosomal dye (LysoTracker® Red). White arrows show LysoTracker® Red-positive acidic organelles, and white arrowheads show NTHi. Fluorescent micrographs were taken at 2,000× magnification. White bars represent 5 μm. Another strain (HUSM 0481) was also tested and similar results were obtained
    Figure Legend Snippet: Localization of intracellular NTHi. BEAS-2B cells were infected with NTHi strain ATCC 19418 for 2 hours. After killing extracellular bacteria with gentamicin, epithelial cells and bacteria were stained with several fluorescent dyes and Hoechst (blue). a Representative fluorescent micrographs of intracellular NTHi (blue) and epithelial cells stained with early endosomal marker (EEA-1, red). White arrows show EEA-1-positive regions, and white arrowheads show NTHi. For a detail of merged image, see a top-right inset. b Representative fluorescence micrographs of intracellular NTHi (blue) and epithelial cells stained with late endosomal marker (LAMP-1, purple). White arrows show LAMP-1-positive regions, and white arrowheads show NTHi. For a detail of merged image, see a top-right inset. c Representative fluorescent micrographs of intracellular NTHi and epithelial cells stained with LIVE/DEAD® (green) and cells were stained with acidic lysosomal dye (LysoTracker® Red). White arrows show LysoTracker® Red-positive acidic organelles, and white arrowheads show NTHi. Fluorescent micrographs were taken at 2,000× magnification. White bars represent 5 μm. Another strain (HUSM 0481) was also tested and similar results were obtained

    Techniques Used: Infection, Staining, Marker, Fluorescence

    NTHi binding of immobilized vitronectin in the absence or presence of heparin. Human plasma vitronectin was bound to glass-bottomed dishes. NTHi was incubated on the plate-bound vitronectin for 30 minutes, and then the dishes were washed three times. In some experiments, plate-bound vitronectin was pretreated with heparin before incubation with NTHi. NTHi was stained with LIVE/DEAD®. Live NTHi is stained green. BSA was used as a negative control. Representative fluorescent micrographs show that both NTHi strains (ATCC 19418 ( a ) and HUSM 0481 ( b )) attached to plate-bound vitronectin and that this attachment is blocked by heparin. White bars represent 10 μm. Summaries of the numbers of attached NTHi (per field at 1,000× magnification) are shown in ( c ) for ATCC 19418 and ( d ) for HUSM 0481. Error bars represent SEM in three independent experiments that gave similar results. ** p < 0.01
    Figure Legend Snippet: NTHi binding of immobilized vitronectin in the absence or presence of heparin. Human plasma vitronectin was bound to glass-bottomed dishes. NTHi was incubated on the plate-bound vitronectin for 30 minutes, and then the dishes were washed three times. In some experiments, plate-bound vitronectin was pretreated with heparin before incubation with NTHi. NTHi was stained with LIVE/DEAD®. Live NTHi is stained green. BSA was used as a negative control. Representative fluorescent micrographs show that both NTHi strains (ATCC 19418 ( a ) and HUSM 0481 ( b )) attached to plate-bound vitronectin and that this attachment is blocked by heparin. White bars represent 10 μm. Summaries of the numbers of attached NTHi (per field at 1,000× magnification) are shown in ( c ) for ATCC 19418 and ( d ) for HUSM 0481. Error bars represent SEM in three independent experiments that gave similar results. ** p < 0.01

    Techniques Used: Binding Assay, Incubation, Staining, Negative Control

    Intracellular invasion of NTHi in the presence of heparin or RGD peptide. BEAS-2B cells were infected for 2 hours with one of the two NTHi strains (ATCC 19418 or HUSM 0481), E. coli (a negative control), or L. monocytogenes (a positive control). In some experiments, cells were pretreated with heparin or RGD-peptide. a After killing extracellular bacteria with gentamicin, epithelial cells and bacteria were stained with LIVE/DEAD®. The percentages of BEAS-2B cells invaded by each type of bacteria are shown. b After killing extracellular bacteria with gentamicin and lysing the BEAS-2B cells, the bacteria were cultured overnight. The number of colonies was counted and the percentages of CFU after gentamicin treatment of cells per input CFU were shown. Error bars represent SEM in three independent experiments that gave similar results. * p < 0.05 and ** p < 0.01
    Figure Legend Snippet: Intracellular invasion of NTHi in the presence of heparin or RGD peptide. BEAS-2B cells were infected for 2 hours with one of the two NTHi strains (ATCC 19418 or HUSM 0481), E. coli (a negative control), or L. monocytogenes (a positive control). In some experiments, cells were pretreated with heparin or RGD-peptide. a After killing extracellular bacteria with gentamicin, epithelial cells and bacteria were stained with LIVE/DEAD®. The percentages of BEAS-2B cells invaded by each type of bacteria are shown. b After killing extracellular bacteria with gentamicin and lysing the BEAS-2B cells, the bacteria were cultured overnight. The number of colonies was counted and the percentages of CFU after gentamicin treatment of cells per input CFU were shown. Error bars represent SEM in three independent experiments that gave similar results. * p < 0.05 and ** p < 0.01

    Techniques Used: Infection, Negative Control, Positive Control, Staining, Cell Culture

    Intracellular invasion of NTHi in the absence or presence of protein-E. BEAS-2B cells were infected for 2 hours with one of the two NTHi strains (ATCC 19418 or HUSM 0481) with or without pretreatment with PE 84–108 peptide. a After killing extracellular bacteria with gentamicin, epithelial cells and bacteria were stained with LIVE/DEAD®. The percentages of BEAS-2B cells invaded by each NTHi strain are shown. b After killing extracellular bacteria with gentamicin, cells were lysed and the bacteria were cultured overnight. The number of colonies was counted and the percentages of CFU after gentamicin treatment of cells per input CFU were shown. Error bars represent SEM in three independent experiments that gave similar results. * p < 0.05 and ** p < 0.01
    Figure Legend Snippet: Intracellular invasion of NTHi in the absence or presence of protein-E. BEAS-2B cells were infected for 2 hours with one of the two NTHi strains (ATCC 19418 or HUSM 0481) with or without pretreatment with PE 84–108 peptide. a After killing extracellular bacteria with gentamicin, epithelial cells and bacteria were stained with LIVE/DEAD®. The percentages of BEAS-2B cells invaded by each NTHi strain are shown. b After killing extracellular bacteria with gentamicin, cells were lysed and the bacteria were cultured overnight. The number of colonies was counted and the percentages of CFU after gentamicin treatment of cells per input CFU were shown. Error bars represent SEM in three independent experiments that gave similar results. * p < 0.05 and ** p < 0.01

    Techniques Used: Infection, Staining, Cell Culture

    nthi strains  (ATCC)


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    ATCC nthi strains
    <t>NTHi</t> penetration of bronchial epithelial <t>cells.</t> <t>BEAS-2B</t> cells were infected with one of two NTHi strains (ATCC 19418 or HUSM 0481), E. coli (a negative control), or L. monocytogenes (a positive control) for 2 hours. After killing extracellular bacteria with gentamicin, epithelial cells and bacteria were stained with LIVE/DEAD® and Hoechst. a Representative fluorescence micrographs of L. monocytogenes , and NTHi strain ATCC 19418. White arrows show viable intracellular bacteria stained green. White bars represent 5 μm. Fluorescent micrographs were taken at 2,000× magnification. b The percentages of epithelial cells invaded by bacteria. c After killing extracellular bacteria with gentamicin, cells were lysed and the bacteria were cultured overnight. The number of colonies was counted and the percentages of CFU after gentamicin treatment per input CFU were shown. Error bars represent SEM in three independent experiments that gave similar results. * p < 0.05 and ** p < 0.01
    Nthi Strains, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nthi strains/product/ATCC
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    nthi strains - by Bioz Stars, 2024-02
    99/100 stars

    Images

    1) Product Images from "Nontypeable Haemophilus influenzae exploits the interaction between protein-E and vitronectin for the adherence and invasion to bronchial epithelial cells"

    Article Title: Nontypeable Haemophilus influenzae exploits the interaction between protein-E and vitronectin for the adherence and invasion to bronchial epithelial cells

    Journal: BMC Microbiology

    doi: 10.1186/s12866-015-0600-8

    NTHi penetration of bronchial epithelial cells. BEAS-2B cells were infected with one of two NTHi strains (ATCC 19418 or HUSM 0481), E. coli (a negative control), or L. monocytogenes (a positive control) for 2 hours. After killing extracellular bacteria with gentamicin, epithelial cells and bacteria were stained with LIVE/DEAD® and Hoechst. a Representative fluorescence micrographs of L. monocytogenes , and NTHi strain ATCC 19418. White arrows show viable intracellular bacteria stained green. White bars represent 5 μm. Fluorescent micrographs were taken at 2,000× magnification. b The percentages of epithelial cells invaded by bacteria. c After killing extracellular bacteria with gentamicin, cells were lysed and the bacteria were cultured overnight. The number of colonies was counted and the percentages of CFU after gentamicin treatment per input CFU were shown. Error bars represent SEM in three independent experiments that gave similar results. * p < 0.05 and ** p < 0.01
    Figure Legend Snippet: NTHi penetration of bronchial epithelial cells. BEAS-2B cells were infected with one of two NTHi strains (ATCC 19418 or HUSM 0481), E. coli (a negative control), or L. monocytogenes (a positive control) for 2 hours. After killing extracellular bacteria with gentamicin, epithelial cells and bacteria were stained with LIVE/DEAD® and Hoechst. a Representative fluorescence micrographs of L. monocytogenes , and NTHi strain ATCC 19418. White arrows show viable intracellular bacteria stained green. White bars represent 5 μm. Fluorescent micrographs were taken at 2,000× magnification. b The percentages of epithelial cells invaded by bacteria. c After killing extracellular bacteria with gentamicin, cells were lysed and the bacteria were cultured overnight. The number of colonies was counted and the percentages of CFU after gentamicin treatment per input CFU were shown. Error bars represent SEM in three independent experiments that gave similar results. * p < 0.05 and ** p < 0.01

    Techniques Used: Infection, Negative Control, Positive Control, Staining, Fluorescence, Cell Culture

    Localization of intracellular NTHi. BEAS-2B cells were infected with NTHi strain ATCC 19418 for 2 hours. After killing extracellular bacteria with gentamicin, epithelial cells and bacteria were stained with several fluorescent dyes and Hoechst (blue). a Representative fluorescent micrographs of intracellular NTHi (blue) and epithelial cells stained with early endosomal marker (EEA-1, red). White arrows show EEA-1-positive regions, and white arrowheads show NTHi. For a detail of merged image, see a top-right inset. b Representative fluorescence micrographs of intracellular NTHi (blue) and epithelial cells stained with late endosomal marker (LAMP-1, purple). White arrows show LAMP-1-positive regions, and white arrowheads show NTHi. For a detail of merged image, see a top-right inset. c Representative fluorescent micrographs of intracellular NTHi and epithelial cells stained with LIVE/DEAD® (green) and cells were stained with acidic lysosomal dye (LysoTracker® Red). White arrows show LysoTracker® Red-positive acidic organelles, and white arrowheads show NTHi. Fluorescent micrographs were taken at 2,000× magnification. White bars represent 5 μm. Another strain (HUSM 0481) was also tested and similar results were obtained
    Figure Legend Snippet: Localization of intracellular NTHi. BEAS-2B cells were infected with NTHi strain ATCC 19418 for 2 hours. After killing extracellular bacteria with gentamicin, epithelial cells and bacteria were stained with several fluorescent dyes and Hoechst (blue). a Representative fluorescent micrographs of intracellular NTHi (blue) and epithelial cells stained with early endosomal marker (EEA-1, red). White arrows show EEA-1-positive regions, and white arrowheads show NTHi. For a detail of merged image, see a top-right inset. b Representative fluorescence micrographs of intracellular NTHi (blue) and epithelial cells stained with late endosomal marker (LAMP-1, purple). White arrows show LAMP-1-positive regions, and white arrowheads show NTHi. For a detail of merged image, see a top-right inset. c Representative fluorescent micrographs of intracellular NTHi and epithelial cells stained with LIVE/DEAD® (green) and cells were stained with acidic lysosomal dye (LysoTracker® Red). White arrows show LysoTracker® Red-positive acidic organelles, and white arrowheads show NTHi. Fluorescent micrographs were taken at 2,000× magnification. White bars represent 5 μm. Another strain (HUSM 0481) was also tested and similar results were obtained

    Techniques Used: Infection, Staining, Marker, Fluorescence

    NTHi binding of immobilized vitronectin in the absence or presence of heparin. Human plasma vitronectin was bound to glass-bottomed dishes. NTHi was incubated on the plate-bound vitronectin for 30 minutes, and then the dishes were washed three times. In some experiments, plate-bound vitronectin was pretreated with heparin before incubation with NTHi. NTHi was stained with LIVE/DEAD®. Live NTHi is stained green. BSA was used as a negative control. Representative fluorescent micrographs show that both NTHi strains (ATCC 19418 ( a ) and HUSM 0481 ( b )) attached to plate-bound vitronectin and that this attachment is blocked by heparin. White bars represent 10 μm. Summaries of the numbers of attached NTHi (per field at 1,000× magnification) are shown in ( c ) for ATCC 19418 and ( d ) for HUSM 0481. Error bars represent SEM in three independent experiments that gave similar results. ** p < 0.01
    Figure Legend Snippet: NTHi binding of immobilized vitronectin in the absence or presence of heparin. Human plasma vitronectin was bound to glass-bottomed dishes. NTHi was incubated on the plate-bound vitronectin for 30 minutes, and then the dishes were washed three times. In some experiments, plate-bound vitronectin was pretreated with heparin before incubation with NTHi. NTHi was stained with LIVE/DEAD®. Live NTHi is stained green. BSA was used as a negative control. Representative fluorescent micrographs show that both NTHi strains (ATCC 19418 ( a ) and HUSM 0481 ( b )) attached to plate-bound vitronectin and that this attachment is blocked by heparin. White bars represent 10 μm. Summaries of the numbers of attached NTHi (per field at 1,000× magnification) are shown in ( c ) for ATCC 19418 and ( d ) for HUSM 0481. Error bars represent SEM in three independent experiments that gave similar results. ** p < 0.01

    Techniques Used: Binding Assay, Incubation, Staining, Negative Control

    Intracellular invasion of NTHi in the presence of heparin or RGD peptide. BEAS-2B cells were infected for 2 hours with one of the two NTHi strains (ATCC 19418 or HUSM 0481), E. coli (a negative control), or L. monocytogenes (a positive control). In some experiments, cells were pretreated with heparin or RGD-peptide. a After killing extracellular bacteria with gentamicin, epithelial cells and bacteria were stained with LIVE/DEAD®. The percentages of BEAS-2B cells invaded by each type of bacteria are shown. b After killing extracellular bacteria with gentamicin and lysing the BEAS-2B cells, the bacteria were cultured overnight. The number of colonies was counted and the percentages of CFU after gentamicin treatment of cells per input CFU were shown. Error bars represent SEM in three independent experiments that gave similar results. * p < 0.05 and ** p < 0.01
    Figure Legend Snippet: Intracellular invasion of NTHi in the presence of heparin or RGD peptide. BEAS-2B cells were infected for 2 hours with one of the two NTHi strains (ATCC 19418 or HUSM 0481), E. coli (a negative control), or L. monocytogenes (a positive control). In some experiments, cells were pretreated with heparin or RGD-peptide. a After killing extracellular bacteria with gentamicin, epithelial cells and bacteria were stained with LIVE/DEAD®. The percentages of BEAS-2B cells invaded by each type of bacteria are shown. b After killing extracellular bacteria with gentamicin and lysing the BEAS-2B cells, the bacteria were cultured overnight. The number of colonies was counted and the percentages of CFU after gentamicin treatment of cells per input CFU were shown. Error bars represent SEM in three independent experiments that gave similar results. * p < 0.05 and ** p < 0.01

    Techniques Used: Infection, Negative Control, Positive Control, Staining, Cell Culture

    Binding of NTHi to immobilized vitronectin in the presence of protein-E peptide. Human plasma vitronectin was bound to glass-bottomed dishes. NTHi (HUSM 0481) was incubated on the plate-bound vitronectin for 30 minutes, and then the dishes were washed three times. In some experiments, plate-bound vitronectin was pretreated with protein-E peptide (PE 84–108 ) before incubation with NTHi. BSA was used as a negative control. NTHi was stained with LIVE/DEAD®, and viable NTHi is stained green. a Representative fluorescent micrographs of NTHi incubated on plate-bound vitronectin that was untreated or pretreated with PE 84–108 . White bars represent 10 μm. b The number of attached NTHi per field at 1,000 × magnification that was pretreated with increasing concentrations of PE 84–108 . Error bars represent SEM in three independent experiments that gave similar results. ** p < 0.01
    Figure Legend Snippet: Binding of NTHi to immobilized vitronectin in the presence of protein-E peptide. Human plasma vitronectin was bound to glass-bottomed dishes. NTHi (HUSM 0481) was incubated on the plate-bound vitronectin for 30 minutes, and then the dishes were washed three times. In some experiments, plate-bound vitronectin was pretreated with protein-E peptide (PE 84–108 ) before incubation with NTHi. BSA was used as a negative control. NTHi was stained with LIVE/DEAD®, and viable NTHi is stained green. a Representative fluorescent micrographs of NTHi incubated on plate-bound vitronectin that was untreated or pretreated with PE 84–108 . White bars represent 10 μm. b The number of attached NTHi per field at 1,000 × magnification that was pretreated with increasing concentrations of PE 84–108 . Error bars represent SEM in three independent experiments that gave similar results. ** p < 0.01

    Techniques Used: Binding Assay, Incubation, Negative Control, Staining

    Intracellular invasion of NTHi in the absence or presence of protein-E. BEAS-2B cells were infected for 2 hours with one of the two NTHi strains (ATCC 19418 or HUSM 0481) with or without pretreatment with PE 84–108 peptide. a After killing extracellular bacteria with gentamicin, epithelial cells and bacteria were stained with LIVE/DEAD®. The percentages of BEAS-2B cells invaded by each NTHi strain are shown. b After killing extracellular bacteria with gentamicin, cells were lysed and the bacteria were cultured overnight. The number of colonies was counted and the percentages of CFU after gentamicin treatment of cells per input CFU were shown. Error bars represent SEM in three independent experiments that gave similar results. * p < 0.05 and ** p < 0.01
    Figure Legend Snippet: Intracellular invasion of NTHi in the absence or presence of protein-E. BEAS-2B cells were infected for 2 hours with one of the two NTHi strains (ATCC 19418 or HUSM 0481) with or without pretreatment with PE 84–108 peptide. a After killing extracellular bacteria with gentamicin, epithelial cells and bacteria were stained with LIVE/DEAD®. The percentages of BEAS-2B cells invaded by each NTHi strain are shown. b After killing extracellular bacteria with gentamicin, cells were lysed and the bacteria were cultured overnight. The number of colonies was counted and the percentages of CFU after gentamicin treatment of cells per input CFU were shown. Error bars represent SEM in three independent experiments that gave similar results. * p < 0.05 and ** p < 0.01

    Techniques Used: Infection, Staining, Cell Culture

    nthi strain atcc 19418  (ATCC)


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    ATCC nthi strain atcc 19418
    NTHi penetration of bronchial epithelial cells. BEAS-2B cells were infected with one of two NTHi strains (ATCC 19418 or HUSM 0481), E. coli (a negative control), or L. monocytogenes (a positive control) for 2 hours. After killing extracellular bacteria with gentamicin, epithelial cells and bacteria were stained with LIVE/DEAD® and Hoechst. a Representative fluorescence micrographs of L. monocytogenes , and NTHi strain ATCC 19418. White arrows show viable intracellular bacteria stained green. White bars represent 5 μm. Fluorescent micrographs were taken at 2,000× magnification. b The percentages of epithelial cells invaded by bacteria. c After killing extracellular bacteria with gentamicin, cells were lysed and the bacteria were cultured overnight. The number of colonies was counted and the percentages of CFU after gentamicin treatment per input CFU were shown. Error bars represent SEM in three independent experiments that gave similar results. * p < 0.05 and ** p < 0.01
    Nthi Strain Atcc 19418, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nthi strain atcc 19418/product/ATCC
    Average 99 stars, based on 1 article reviews
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    1) Product Images from "Nontypeable Haemophilus influenzae exploits the interaction between protein-E and vitronectin for the adherence and invasion to bronchial epithelial cells"

    Article Title: Nontypeable Haemophilus influenzae exploits the interaction between protein-E and vitronectin for the adherence and invasion to bronchial epithelial cells

    Journal: BMC Microbiology

    doi: 10.1186/s12866-015-0600-8

    NTHi penetration of bronchial epithelial cells. BEAS-2B cells were infected with one of two NTHi strains (ATCC 19418 or HUSM 0481), E. coli (a negative control), or L. monocytogenes (a positive control) for 2 hours. After killing extracellular bacteria with gentamicin, epithelial cells and bacteria were stained with LIVE/DEAD® and Hoechst. a Representative fluorescence micrographs of L. monocytogenes , and NTHi strain ATCC 19418. White arrows show viable intracellular bacteria stained green. White bars represent 5 μm. Fluorescent micrographs were taken at 2,000× magnification. b The percentages of epithelial cells invaded by bacteria. c After killing extracellular bacteria with gentamicin, cells were lysed and the bacteria were cultured overnight. The number of colonies was counted and the percentages of CFU after gentamicin treatment per input CFU were shown. Error bars represent SEM in three independent experiments that gave similar results. * p < 0.05 and ** p < 0.01
    Figure Legend Snippet: NTHi penetration of bronchial epithelial cells. BEAS-2B cells were infected with one of two NTHi strains (ATCC 19418 or HUSM 0481), E. coli (a negative control), or L. monocytogenes (a positive control) for 2 hours. After killing extracellular bacteria with gentamicin, epithelial cells and bacteria were stained with LIVE/DEAD® and Hoechst. a Representative fluorescence micrographs of L. monocytogenes , and NTHi strain ATCC 19418. White arrows show viable intracellular bacteria stained green. White bars represent 5 μm. Fluorescent micrographs were taken at 2,000× magnification. b The percentages of epithelial cells invaded by bacteria. c After killing extracellular bacteria with gentamicin, cells were lysed and the bacteria were cultured overnight. The number of colonies was counted and the percentages of CFU after gentamicin treatment per input CFU were shown. Error bars represent SEM in three independent experiments that gave similar results. * p < 0.05 and ** p < 0.01

    Techniques Used: Infection, Negative Control, Positive Control, Staining, Fluorescence, Cell Culture

    Localization of intracellular NTHi. BEAS-2B cells were infected with NTHi strain ATCC 19418 for 2 hours. After killing extracellular bacteria with gentamicin, epithelial cells and bacteria were stained with several fluorescent dyes and Hoechst (blue). a Representative fluorescent micrographs of intracellular NTHi (blue) and epithelial cells stained with early endosomal marker (EEA-1, red). White arrows show EEA-1-positive regions, and white arrowheads show NTHi. For a detail of merged image, see a top-right inset. b Representative fluorescence micrographs of intracellular NTHi (blue) and epithelial cells stained with late endosomal marker (LAMP-1, purple). White arrows show LAMP-1-positive regions, and white arrowheads show NTHi. For a detail of merged image, see a top-right inset. c Representative fluorescent micrographs of intracellular NTHi and epithelial cells stained with LIVE/DEAD® (green) and cells were stained with acidic lysosomal dye (LysoTracker® Red). White arrows show LysoTracker® Red-positive acidic organelles, and white arrowheads show NTHi. Fluorescent micrographs were taken at 2,000× magnification. White bars represent 5 μm. Another strain (HUSM 0481) was also tested and similar results were obtained
    Figure Legend Snippet: Localization of intracellular NTHi. BEAS-2B cells were infected with NTHi strain ATCC 19418 for 2 hours. After killing extracellular bacteria with gentamicin, epithelial cells and bacteria were stained with several fluorescent dyes and Hoechst (blue). a Representative fluorescent micrographs of intracellular NTHi (blue) and epithelial cells stained with early endosomal marker (EEA-1, red). White arrows show EEA-1-positive regions, and white arrowheads show NTHi. For a detail of merged image, see a top-right inset. b Representative fluorescence micrographs of intracellular NTHi (blue) and epithelial cells stained with late endosomal marker (LAMP-1, purple). White arrows show LAMP-1-positive regions, and white arrowheads show NTHi. For a detail of merged image, see a top-right inset. c Representative fluorescent micrographs of intracellular NTHi and epithelial cells stained with LIVE/DEAD® (green) and cells were stained with acidic lysosomal dye (LysoTracker® Red). White arrows show LysoTracker® Red-positive acidic organelles, and white arrowheads show NTHi. Fluorescent micrographs were taken at 2,000× magnification. White bars represent 5 μm. Another strain (HUSM 0481) was also tested and similar results were obtained

    Techniques Used: Infection, Staining, Marker, Fluorescence

    NTHi binding of immobilized vitronectin in the absence or presence of heparin. Human plasma vitronectin was bound to glass-bottomed dishes. NTHi was incubated on the plate-bound vitronectin for 30 minutes, and then the dishes were washed three times. In some experiments, plate-bound vitronectin was pretreated with heparin before incubation with NTHi. NTHi was stained with LIVE/DEAD®. Live NTHi is stained green. BSA was used as a negative control. Representative fluorescent micrographs show that both NTHi strains (ATCC 19418 ( a ) and HUSM 0481 ( b )) attached to plate-bound vitronectin and that this attachment is blocked by heparin. White bars represent 10 μm. Summaries of the numbers of attached NTHi (per field at 1,000× magnification) are shown in ( c ) for ATCC 19418 and ( d ) for HUSM 0481. Error bars represent SEM in three independent experiments that gave similar results. ** p < 0.01
    Figure Legend Snippet: NTHi binding of immobilized vitronectin in the absence or presence of heparin. Human plasma vitronectin was bound to glass-bottomed dishes. NTHi was incubated on the plate-bound vitronectin for 30 minutes, and then the dishes were washed three times. In some experiments, plate-bound vitronectin was pretreated with heparin before incubation with NTHi. NTHi was stained with LIVE/DEAD®. Live NTHi is stained green. BSA was used as a negative control. Representative fluorescent micrographs show that both NTHi strains (ATCC 19418 ( a ) and HUSM 0481 ( b )) attached to plate-bound vitronectin and that this attachment is blocked by heparin. White bars represent 10 μm. Summaries of the numbers of attached NTHi (per field at 1,000× magnification) are shown in ( c ) for ATCC 19418 and ( d ) for HUSM 0481. Error bars represent SEM in three independent experiments that gave similar results. ** p < 0.01

    Techniques Used: Binding Assay, Incubation, Staining, Negative Control

    Intracellular invasion of NTHi in the presence of heparin or RGD peptide. BEAS-2B cells were infected for 2 hours with one of the two NTHi strains (ATCC 19418 or HUSM 0481), E. coli (a negative control), or L. monocytogenes (a positive control). In some experiments, cells were pretreated with heparin or RGD-peptide. a After killing extracellular bacteria with gentamicin, epithelial cells and bacteria were stained with LIVE/DEAD®. The percentages of BEAS-2B cells invaded by each type of bacteria are shown. b After killing extracellular bacteria with gentamicin and lysing the BEAS-2B cells, the bacteria were cultured overnight. The number of colonies was counted and the percentages of CFU after gentamicin treatment of cells per input CFU were shown. Error bars represent SEM in three independent experiments that gave similar results. * p < 0.05 and ** p < 0.01
    Figure Legend Snippet: Intracellular invasion of NTHi in the presence of heparin or RGD peptide. BEAS-2B cells were infected for 2 hours with one of the two NTHi strains (ATCC 19418 or HUSM 0481), E. coli (a negative control), or L. monocytogenes (a positive control). In some experiments, cells were pretreated with heparin or RGD-peptide. a After killing extracellular bacteria with gentamicin, epithelial cells and bacteria were stained with LIVE/DEAD®. The percentages of BEAS-2B cells invaded by each type of bacteria are shown. b After killing extracellular bacteria with gentamicin and lysing the BEAS-2B cells, the bacteria were cultured overnight. The number of colonies was counted and the percentages of CFU after gentamicin treatment of cells per input CFU were shown. Error bars represent SEM in three independent experiments that gave similar results. * p < 0.05 and ** p < 0.01

    Techniques Used: Infection, Negative Control, Positive Control, Staining, Cell Culture

    Intracellular invasion of NTHi in the absence or presence of protein-E. BEAS-2B cells were infected for 2 hours with one of the two NTHi strains (ATCC 19418 or HUSM 0481) with or without pretreatment with PE 84–108 peptide. a After killing extracellular bacteria with gentamicin, epithelial cells and bacteria were stained with LIVE/DEAD®. The percentages of BEAS-2B cells invaded by each NTHi strain are shown. b After killing extracellular bacteria with gentamicin, cells were lysed and the bacteria were cultured overnight. The number of colonies was counted and the percentages of CFU after gentamicin treatment of cells per input CFU were shown. Error bars represent SEM in three independent experiments that gave similar results. * p < 0.05 and ** p < 0.01
    Figure Legend Snippet: Intracellular invasion of NTHi in the absence or presence of protein-E. BEAS-2B cells were infected for 2 hours with one of the two NTHi strains (ATCC 19418 or HUSM 0481) with or without pretreatment with PE 84–108 peptide. a After killing extracellular bacteria with gentamicin, epithelial cells and bacteria were stained with LIVE/DEAD®. The percentages of BEAS-2B cells invaded by each NTHi strain are shown. b After killing extracellular bacteria with gentamicin, cells were lysed and the bacteria were cultured overnight. The number of colonies was counted and the percentages of CFU after gentamicin treatment of cells per input CFU were shown. Error bars represent SEM in three independent experiments that gave similar results. * p < 0.05 and ** p < 0.01

    Techniques Used: Infection, Staining, Cell Culture

    nthi strain atcc 19418  (ATCC)


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    Structured Review

    ATCC nthi strain atcc 19418
    NTHi penetration of bronchial epithelial cells. BEAS-2B cells were infected with one of two NTHi strains (ATCC 19418 or HUSM 0481), E. coli (a negative control), or L. monocytogenes (a positive control) for 2 hours. After killing extracellular bacteria with gentamicin, epithelial cells and bacteria were stained with LIVE/DEAD® and Hoechst. a Representative fluorescence micrographs of L. monocytogenes , and NTHi strain ATCC 19418. White arrows show viable intracellular bacteria stained green. White bars represent 5 μm. Fluorescent micrographs were taken at 2,000× magnification. b The percentages of epithelial cells invaded by bacteria. c After killing extracellular bacteria with gentamicin, cells were lysed and the bacteria were cultured overnight. The number of colonies was counted and the percentages of CFU after gentamicin treatment per input CFU were shown. Error bars represent SEM in three independent experiments that gave similar results. * p < 0.05 and ** p < 0.01
    Nthi Strain Atcc 19418, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nthi strain atcc 19418/product/ATCC
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    nthi strain atcc 19418 - by Bioz Stars, 2024-02
    99/100 stars

    Images

    1) Product Images from "Nontypeable Haemophilus influenzae exploits the interaction between protein-E and vitronectin for the adherence and invasion to bronchial epithelial cells"

    Article Title: Nontypeable Haemophilus influenzae exploits the interaction between protein-E and vitronectin for the adherence and invasion to bronchial epithelial cells

    Journal: BMC Microbiology

    doi: 10.1186/s12866-015-0600-8

    NTHi penetration of bronchial epithelial cells. BEAS-2B cells were infected with one of two NTHi strains (ATCC 19418 or HUSM 0481), E. coli (a negative control), or L. monocytogenes (a positive control) for 2 hours. After killing extracellular bacteria with gentamicin, epithelial cells and bacteria were stained with LIVE/DEAD® and Hoechst. a Representative fluorescence micrographs of L. monocytogenes , and NTHi strain ATCC 19418. White arrows show viable intracellular bacteria stained green. White bars represent 5 μm. Fluorescent micrographs were taken at 2,000× magnification. b The percentages of epithelial cells invaded by bacteria. c After killing extracellular bacteria with gentamicin, cells were lysed and the bacteria were cultured overnight. The number of colonies was counted and the percentages of CFU after gentamicin treatment per input CFU were shown. Error bars represent SEM in three independent experiments that gave similar results. * p < 0.05 and ** p < 0.01
    Figure Legend Snippet: NTHi penetration of bronchial epithelial cells. BEAS-2B cells were infected with one of two NTHi strains (ATCC 19418 or HUSM 0481), E. coli (a negative control), or L. monocytogenes (a positive control) for 2 hours. After killing extracellular bacteria with gentamicin, epithelial cells and bacteria were stained with LIVE/DEAD® and Hoechst. a Representative fluorescence micrographs of L. monocytogenes , and NTHi strain ATCC 19418. White arrows show viable intracellular bacteria stained green. White bars represent 5 μm. Fluorescent micrographs were taken at 2,000× magnification. b The percentages of epithelial cells invaded by bacteria. c After killing extracellular bacteria with gentamicin, cells were lysed and the bacteria were cultured overnight. The number of colonies was counted and the percentages of CFU after gentamicin treatment per input CFU were shown. Error bars represent SEM in three independent experiments that gave similar results. * p < 0.05 and ** p < 0.01

    Techniques Used: Infection, Negative Control, Positive Control, Staining, Fluorescence, Cell Culture

    Localization of intracellular NTHi. BEAS-2B cells were infected with NTHi strain ATCC 19418 for 2 hours. After killing extracellular bacteria with gentamicin, epithelial cells and bacteria were stained with several fluorescent dyes and Hoechst (blue). a Representative fluorescent micrographs of intracellular NTHi (blue) and epithelial cells stained with early endosomal marker (EEA-1, red). White arrows show EEA-1-positive regions, and white arrowheads show NTHi. For a detail of merged image, see a top-right inset. b Representative fluorescence micrographs of intracellular NTHi (blue) and epithelial cells stained with late endosomal marker (LAMP-1, purple). White arrows show LAMP-1-positive regions, and white arrowheads show NTHi. For a detail of merged image, see a top-right inset. c Representative fluorescent micrographs of intracellular NTHi and epithelial cells stained with LIVE/DEAD® (green) and cells were stained with acidic lysosomal dye (LysoTracker® Red). White arrows show LysoTracker® Red-positive acidic organelles, and white arrowheads show NTHi. Fluorescent micrographs were taken at 2,000× magnification. White bars represent 5 μm. Another strain (HUSM 0481) was also tested and similar results were obtained
    Figure Legend Snippet: Localization of intracellular NTHi. BEAS-2B cells were infected with NTHi strain ATCC 19418 for 2 hours. After killing extracellular bacteria with gentamicin, epithelial cells and bacteria were stained with several fluorescent dyes and Hoechst (blue). a Representative fluorescent micrographs of intracellular NTHi (blue) and epithelial cells stained with early endosomal marker (EEA-1, red). White arrows show EEA-1-positive regions, and white arrowheads show NTHi. For a detail of merged image, see a top-right inset. b Representative fluorescence micrographs of intracellular NTHi (blue) and epithelial cells stained with late endosomal marker (LAMP-1, purple). White arrows show LAMP-1-positive regions, and white arrowheads show NTHi. For a detail of merged image, see a top-right inset. c Representative fluorescent micrographs of intracellular NTHi and epithelial cells stained with LIVE/DEAD® (green) and cells were stained with acidic lysosomal dye (LysoTracker® Red). White arrows show LysoTracker® Red-positive acidic organelles, and white arrowheads show NTHi. Fluorescent micrographs were taken at 2,000× magnification. White bars represent 5 μm. Another strain (HUSM 0481) was also tested and similar results were obtained

    Techniques Used: Infection, Staining, Marker, Fluorescence

    NTHi binding of immobilized vitronectin in the absence or presence of heparin. Human plasma vitronectin was bound to glass-bottomed dishes. NTHi was incubated on the plate-bound vitronectin for 30 minutes, and then the dishes were washed three times. In some experiments, plate-bound vitronectin was pretreated with heparin before incubation with NTHi. NTHi was stained with LIVE/DEAD®. Live NTHi is stained green. BSA was used as a negative control. Representative fluorescent micrographs show that both NTHi strains (ATCC 19418 ( a ) and HUSM 0481 ( b )) attached to plate-bound vitronectin and that this attachment is blocked by heparin. White bars represent 10 μm. Summaries of the numbers of attached NTHi (per field at 1,000× magnification) are shown in ( c ) for ATCC 19418 and ( d ) for HUSM 0481. Error bars represent SEM in three independent experiments that gave similar results. ** p < 0.01
    Figure Legend Snippet: NTHi binding of immobilized vitronectin in the absence or presence of heparin. Human plasma vitronectin was bound to glass-bottomed dishes. NTHi was incubated on the plate-bound vitronectin for 30 minutes, and then the dishes were washed three times. In some experiments, plate-bound vitronectin was pretreated with heparin before incubation with NTHi. NTHi was stained with LIVE/DEAD®. Live NTHi is stained green. BSA was used as a negative control. Representative fluorescent micrographs show that both NTHi strains (ATCC 19418 ( a ) and HUSM 0481 ( b )) attached to plate-bound vitronectin and that this attachment is blocked by heparin. White bars represent 10 μm. Summaries of the numbers of attached NTHi (per field at 1,000× magnification) are shown in ( c ) for ATCC 19418 and ( d ) for HUSM 0481. Error bars represent SEM in three independent experiments that gave similar results. ** p < 0.01

    Techniques Used: Binding Assay, Incubation, Staining, Negative Control

    Intracellular invasion of NTHi in the presence of heparin or RGD peptide. BEAS-2B cells were infected for 2 hours with one of the two NTHi strains (ATCC 19418 or HUSM 0481), E. coli (a negative control), or L. monocytogenes (a positive control). In some experiments, cells were pretreated with heparin or RGD-peptide. a After killing extracellular bacteria with gentamicin, epithelial cells and bacteria were stained with LIVE/DEAD®. The percentages of BEAS-2B cells invaded by each type of bacteria are shown. b After killing extracellular bacteria with gentamicin and lysing the BEAS-2B cells, the bacteria were cultured overnight. The number of colonies was counted and the percentages of CFU after gentamicin treatment of cells per input CFU were shown. Error bars represent SEM in three independent experiments that gave similar results. * p < 0.05 and ** p < 0.01
    Figure Legend Snippet: Intracellular invasion of NTHi in the presence of heparin or RGD peptide. BEAS-2B cells were infected for 2 hours with one of the two NTHi strains (ATCC 19418 or HUSM 0481), E. coli (a negative control), or L. monocytogenes (a positive control). In some experiments, cells were pretreated with heparin or RGD-peptide. a After killing extracellular bacteria with gentamicin, epithelial cells and bacteria were stained with LIVE/DEAD®. The percentages of BEAS-2B cells invaded by each type of bacteria are shown. b After killing extracellular bacteria with gentamicin and lysing the BEAS-2B cells, the bacteria were cultured overnight. The number of colonies was counted and the percentages of CFU after gentamicin treatment of cells per input CFU were shown. Error bars represent SEM in three independent experiments that gave similar results. * p < 0.05 and ** p < 0.01

    Techniques Used: Infection, Negative Control, Positive Control, Staining, Cell Culture

    Intracellular invasion of NTHi in the absence or presence of protein-E. BEAS-2B cells were infected for 2 hours with one of the two NTHi strains (ATCC 19418 or HUSM 0481) with or without pretreatment with PE 84–108 peptide. a After killing extracellular bacteria with gentamicin, epithelial cells and bacteria were stained with LIVE/DEAD®. The percentages of BEAS-2B cells invaded by each NTHi strain are shown. b After killing extracellular bacteria with gentamicin, cells were lysed and the bacteria were cultured overnight. The number of colonies was counted and the percentages of CFU after gentamicin treatment of cells per input CFU were shown. Error bars represent SEM in three independent experiments that gave similar results. * p < 0.05 and ** p < 0.01
    Figure Legend Snippet: Intracellular invasion of NTHi in the absence or presence of protein-E. BEAS-2B cells were infected for 2 hours with one of the two NTHi strains (ATCC 19418 or HUSM 0481) with or without pretreatment with PE 84–108 peptide. a After killing extracellular bacteria with gentamicin, epithelial cells and bacteria were stained with LIVE/DEAD®. The percentages of BEAS-2B cells invaded by each NTHi strain are shown. b After killing extracellular bacteria with gentamicin, cells were lysed and the bacteria were cultured overnight. The number of colonies was counted and the percentages of CFU after gentamicin treatment of cells per input CFU were shown. Error bars represent SEM in three independent experiments that gave similar results. * p < 0.05 and ** p < 0.01

    Techniques Used: Infection, Staining, Cell Culture

    nthi  (ATCC)


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    ATCC nthi
    <t>NTHi</t> penetration of bronchial epithelial cells. BEAS-2B cells were infected with one of two NTHi <t>strains</t> <t>(ATCC</t> 19418 or HUSM 0481), E. coli (a negative control), or L. monocytogenes (a positive control) for 2 hours. After killing extracellular bacteria with gentamicin, epithelial cells and bacteria were stained with LIVE/DEAD® and Hoechst. a Representative fluorescence micrographs of L. monocytogenes , and NTHi strain ATCC 19418. White arrows show viable intracellular bacteria stained green. White bars represent 5 μm. Fluorescent micrographs were taken at 2,000× magnification. b The percentages of epithelial cells invaded by bacteria. c After killing extracellular bacteria with gentamicin, cells were lysed and the bacteria were cultured overnight. The number of colonies was counted and the percentages of CFU after gentamicin treatment per input CFU were shown. Error bars represent SEM in three independent experiments that gave similar results. * p < 0.05 and ** p < 0.01
    Nthi, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nthi/product/ATCC
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    nthi - by Bioz Stars, 2024-02
    99/100 stars

    Images

    1) Product Images from "Nontypeable Haemophilus influenzae exploits the interaction between protein-E and vitronectin for the adherence and invasion to bronchial epithelial cells"

    Article Title: Nontypeable Haemophilus influenzae exploits the interaction between protein-E and vitronectin for the adherence and invasion to bronchial epithelial cells

    Journal: BMC Microbiology

    doi: 10.1186/s12866-015-0600-8

    NTHi penetration of bronchial epithelial cells. BEAS-2B cells were infected with one of two NTHi strains (ATCC 19418 or HUSM 0481), E. coli (a negative control), or L. monocytogenes (a positive control) for 2 hours. After killing extracellular bacteria with gentamicin, epithelial cells and bacteria were stained with LIVE/DEAD® and Hoechst. a Representative fluorescence micrographs of L. monocytogenes , and NTHi strain ATCC 19418. White arrows show viable intracellular bacteria stained green. White bars represent 5 μm. Fluorescent micrographs were taken at 2,000× magnification. b The percentages of epithelial cells invaded by bacteria. c After killing extracellular bacteria with gentamicin, cells were lysed and the bacteria were cultured overnight. The number of colonies was counted and the percentages of CFU after gentamicin treatment per input CFU were shown. Error bars represent SEM in three independent experiments that gave similar results. * p < 0.05 and ** p < 0.01
    Figure Legend Snippet: NTHi penetration of bronchial epithelial cells. BEAS-2B cells were infected with one of two NTHi strains (ATCC 19418 or HUSM 0481), E. coli (a negative control), or L. monocytogenes (a positive control) for 2 hours. After killing extracellular bacteria with gentamicin, epithelial cells and bacteria were stained with LIVE/DEAD® and Hoechst. a Representative fluorescence micrographs of L. monocytogenes , and NTHi strain ATCC 19418. White arrows show viable intracellular bacteria stained green. White bars represent 5 μm. Fluorescent micrographs were taken at 2,000× magnification. b The percentages of epithelial cells invaded by bacteria. c After killing extracellular bacteria with gentamicin, cells were lysed and the bacteria were cultured overnight. The number of colonies was counted and the percentages of CFU after gentamicin treatment per input CFU were shown. Error bars represent SEM in three independent experiments that gave similar results. * p < 0.05 and ** p < 0.01

    Techniques Used: Infection, Negative Control, Positive Control, Staining, Fluorescence, Cell Culture

    Localization of intracellular NTHi. BEAS-2B cells were infected with NTHi strain ATCC 19418 for 2 hours. After killing extracellular bacteria with gentamicin, epithelial cells and bacteria were stained with several fluorescent dyes and Hoechst (blue). a Representative fluorescent micrographs of intracellular NTHi (blue) and epithelial cells stained with early endosomal marker (EEA-1, red). White arrows show EEA-1-positive regions, and white arrowheads show NTHi. For a detail of merged image, see a top-right inset. b Representative fluorescence micrographs of intracellular NTHi (blue) and epithelial cells stained with late endosomal marker (LAMP-1, purple). White arrows show LAMP-1-positive regions, and white arrowheads show NTHi. For a detail of merged image, see a top-right inset. c Representative fluorescent micrographs of intracellular NTHi and epithelial cells stained with LIVE/DEAD® (green) and cells were stained with acidic lysosomal dye (LysoTracker® Red). White arrows show LysoTracker® Red-positive acidic organelles, and white arrowheads show NTHi. Fluorescent micrographs were taken at 2,000× magnification. White bars represent 5 μm. Another strain (HUSM 0481) was also tested and similar results were obtained
    Figure Legend Snippet: Localization of intracellular NTHi. BEAS-2B cells were infected with NTHi strain ATCC 19418 for 2 hours. After killing extracellular bacteria with gentamicin, epithelial cells and bacteria were stained with several fluorescent dyes and Hoechst (blue). a Representative fluorescent micrographs of intracellular NTHi (blue) and epithelial cells stained with early endosomal marker (EEA-1, red). White arrows show EEA-1-positive regions, and white arrowheads show NTHi. For a detail of merged image, see a top-right inset. b Representative fluorescence micrographs of intracellular NTHi (blue) and epithelial cells stained with late endosomal marker (LAMP-1, purple). White arrows show LAMP-1-positive regions, and white arrowheads show NTHi. For a detail of merged image, see a top-right inset. c Representative fluorescent micrographs of intracellular NTHi and epithelial cells stained with LIVE/DEAD® (green) and cells were stained with acidic lysosomal dye (LysoTracker® Red). White arrows show LysoTracker® Red-positive acidic organelles, and white arrowheads show NTHi. Fluorescent micrographs were taken at 2,000× magnification. White bars represent 5 μm. Another strain (HUSM 0481) was also tested and similar results were obtained

    Techniques Used: Infection, Staining, Marker, Fluorescence

    NTHi binding of immobilized vitronectin in the absence or presence of heparin. Human plasma vitronectin was bound to glass-bottomed dishes. NTHi was incubated on the plate-bound vitronectin for 30 minutes, and then the dishes were washed three times. In some experiments, plate-bound vitronectin was pretreated with heparin before incubation with NTHi. NTHi was stained with LIVE/DEAD®. Live NTHi is stained green. BSA was used as a negative control. Representative fluorescent micrographs show that both NTHi strains (ATCC 19418 ( a ) and HUSM 0481 ( b )) attached to plate-bound vitronectin and that this attachment is blocked by heparin. White bars represent 10 μm. Summaries of the numbers of attached NTHi (per field at 1,000× magnification) are shown in ( c ) for ATCC 19418 and ( d ) for HUSM 0481. Error bars represent SEM in three independent experiments that gave similar results. ** p < 0.01
    Figure Legend Snippet: NTHi binding of immobilized vitronectin in the absence or presence of heparin. Human plasma vitronectin was bound to glass-bottomed dishes. NTHi was incubated on the plate-bound vitronectin for 30 minutes, and then the dishes were washed three times. In some experiments, plate-bound vitronectin was pretreated with heparin before incubation with NTHi. NTHi was stained with LIVE/DEAD®. Live NTHi is stained green. BSA was used as a negative control. Representative fluorescent micrographs show that both NTHi strains (ATCC 19418 ( a ) and HUSM 0481 ( b )) attached to plate-bound vitronectin and that this attachment is blocked by heparin. White bars represent 10 μm. Summaries of the numbers of attached NTHi (per field at 1,000× magnification) are shown in ( c ) for ATCC 19418 and ( d ) for HUSM 0481. Error bars represent SEM in three independent experiments that gave similar results. ** p < 0.01

    Techniques Used: Binding Assay, Incubation, Staining, Negative Control

    Intracellular invasion of NTHi in the presence of heparin or RGD peptide. BEAS-2B cells were infected for 2 hours with one of the two NTHi strains (ATCC 19418 or HUSM 0481), E. coli (a negative control), or L. monocytogenes (a positive control). In some experiments, cells were pretreated with heparin or RGD-peptide. a After killing extracellular bacteria with gentamicin, epithelial cells and bacteria were stained with LIVE/DEAD®. The percentages of BEAS-2B cells invaded by each type of bacteria are shown. b After killing extracellular bacteria with gentamicin and lysing the BEAS-2B cells, the bacteria were cultured overnight. The number of colonies was counted and the percentages of CFU after gentamicin treatment of cells per input CFU were shown. Error bars represent SEM in three independent experiments that gave similar results. * p < 0.05 and ** p < 0.01
    Figure Legend Snippet: Intracellular invasion of NTHi in the presence of heparin or RGD peptide. BEAS-2B cells were infected for 2 hours with one of the two NTHi strains (ATCC 19418 or HUSM 0481), E. coli (a negative control), or L. monocytogenes (a positive control). In some experiments, cells were pretreated with heparin or RGD-peptide. a After killing extracellular bacteria with gentamicin, epithelial cells and bacteria were stained with LIVE/DEAD®. The percentages of BEAS-2B cells invaded by each type of bacteria are shown. b After killing extracellular bacteria with gentamicin and lysing the BEAS-2B cells, the bacteria were cultured overnight. The number of colonies was counted and the percentages of CFU after gentamicin treatment of cells per input CFU were shown. Error bars represent SEM in three independent experiments that gave similar results. * p < 0.05 and ** p < 0.01

    Techniques Used: Infection, Negative Control, Positive Control, Staining, Cell Culture

    Binding of NTHi to immobilized vitronectin in the presence of protein-E peptide. Human plasma vitronectin was bound to glass-bottomed dishes. NTHi (HUSM 0481) was incubated on the plate-bound vitronectin for 30 minutes, and then the dishes were washed three times. In some experiments, plate-bound vitronectin was pretreated with protein-E peptide (PE 84–108 ) before incubation with NTHi. BSA was used as a negative control. NTHi was stained with LIVE/DEAD®, and viable NTHi is stained green. a Representative fluorescent micrographs of NTHi incubated on plate-bound vitronectin that was untreated or pretreated with PE 84–108 . White bars represent 10 μm. b The number of attached NTHi per field at 1,000 × magnification that was pretreated with increasing concentrations of PE 84–108 . Error bars represent SEM in three independent experiments that gave similar results. ** p < 0.01
    Figure Legend Snippet: Binding of NTHi to immobilized vitronectin in the presence of protein-E peptide. Human plasma vitronectin was bound to glass-bottomed dishes. NTHi (HUSM 0481) was incubated on the plate-bound vitronectin for 30 minutes, and then the dishes were washed three times. In some experiments, plate-bound vitronectin was pretreated with protein-E peptide (PE 84–108 ) before incubation with NTHi. BSA was used as a negative control. NTHi was stained with LIVE/DEAD®, and viable NTHi is stained green. a Representative fluorescent micrographs of NTHi incubated on plate-bound vitronectin that was untreated or pretreated with PE 84–108 . White bars represent 10 μm. b The number of attached NTHi per field at 1,000 × magnification that was pretreated with increasing concentrations of PE 84–108 . Error bars represent SEM in three independent experiments that gave similar results. ** p < 0.01

    Techniques Used: Binding Assay, Incubation, Negative Control, Staining

    Intracellular invasion of NTHi in the absence or presence of protein-E. BEAS-2B cells were infected for 2 hours with one of the two NTHi strains (ATCC 19418 or HUSM 0481) with or without pretreatment with PE 84–108 peptide. a After killing extracellular bacteria with gentamicin, epithelial cells and bacteria were stained with LIVE/DEAD®. The percentages of BEAS-2B cells invaded by each NTHi strain are shown. b After killing extracellular bacteria with gentamicin, cells were lysed and the bacteria were cultured overnight. The number of colonies was counted and the percentages of CFU after gentamicin treatment of cells per input CFU were shown. Error bars represent SEM in three independent experiments that gave similar results. * p < 0.05 and ** p < 0.01
    Figure Legend Snippet: Intracellular invasion of NTHi in the absence or presence of protein-E. BEAS-2B cells were infected for 2 hours with one of the two NTHi strains (ATCC 19418 or HUSM 0481) with or without pretreatment with PE 84–108 peptide. a After killing extracellular bacteria with gentamicin, epithelial cells and bacteria were stained with LIVE/DEAD®. The percentages of BEAS-2B cells invaded by each NTHi strain are shown. b After killing extracellular bacteria with gentamicin, cells were lysed and the bacteria were cultured overnight. The number of colonies was counted and the percentages of CFU after gentamicin treatment of cells per input CFU were shown. Error bars represent SEM in three independent experiments that gave similar results. * p < 0.05 and ** p < 0.01

    Techniques Used: Infection, Staining, Cell Culture

    nthi strains  (ATCC)


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    Structured Review

    ATCC nthi strains
    <t>NTHi</t> penetration of bronchial epithelial cells. BEAS-2B cells were infected with one of two NTHi strains (ATCC 19418 or HUSM 0481), E. coli (a negative control), or L. monocytogenes (a positive control) for 2 hours. After killing extracellular bacteria with gentamicin, epithelial cells and bacteria were stained with LIVE/DEAD® and Hoechst. a Representative fluorescence micrographs of L. monocytogenes , and NTHi strain ATCC 19418. White arrows show viable intracellular bacteria stained green. White bars represent 5 <t>μm.</t> <t>Fluorescent</t> micrographs were taken at 2,000× magnification. b The percentages of epithelial cells invaded by bacteria. c After killing extracellular bacteria with gentamicin, cells were lysed and the bacteria were cultured overnight. The number of colonies was counted and the percentages of CFU after gentamicin treatment per input CFU were shown. Error bars represent SEM in three independent experiments that gave similar results. * p < 0.05 and ** p < 0.01
    Nthi Strains, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "Nontypeable Haemophilus influenzae exploits the interaction between protein-E and vitronectin for the adherence and invasion to bronchial epithelial cells"

    Article Title: Nontypeable Haemophilus influenzae exploits the interaction between protein-E and vitronectin for the adherence and invasion to bronchial epithelial cells

    Journal: BMC Microbiology

    doi: 10.1186/s12866-015-0600-8

    NTHi penetration of bronchial epithelial cells. BEAS-2B cells were infected with one of two NTHi strains (ATCC 19418 or HUSM 0481), E. coli (a negative control), or L. monocytogenes (a positive control) for 2 hours. After killing extracellular bacteria with gentamicin, epithelial cells and bacteria were stained with LIVE/DEAD® and Hoechst. a Representative fluorescence micrographs of L. monocytogenes , and NTHi strain ATCC 19418. White arrows show viable intracellular bacteria stained green. White bars represent 5 μm. Fluorescent micrographs were taken at 2,000× magnification. b The percentages of epithelial cells invaded by bacteria. c After killing extracellular bacteria with gentamicin, cells were lysed and the bacteria were cultured overnight. The number of colonies was counted and the percentages of CFU after gentamicin treatment per input CFU were shown. Error bars represent SEM in three independent experiments that gave similar results. * p < 0.05 and ** p < 0.01
    Figure Legend Snippet: NTHi penetration of bronchial epithelial cells. BEAS-2B cells were infected with one of two NTHi strains (ATCC 19418 or HUSM 0481), E. coli (a negative control), or L. monocytogenes (a positive control) for 2 hours. After killing extracellular bacteria with gentamicin, epithelial cells and bacteria were stained with LIVE/DEAD® and Hoechst. a Representative fluorescence micrographs of L. monocytogenes , and NTHi strain ATCC 19418. White arrows show viable intracellular bacteria stained green. White bars represent 5 μm. Fluorescent micrographs were taken at 2,000× magnification. b The percentages of epithelial cells invaded by bacteria. c After killing extracellular bacteria with gentamicin, cells were lysed and the bacteria were cultured overnight. The number of colonies was counted and the percentages of CFU after gentamicin treatment per input CFU were shown. Error bars represent SEM in three independent experiments that gave similar results. * p < 0.05 and ** p < 0.01

    Techniques Used: Infection, Negative Control, Positive Control, Staining, Fluorescence, Cell Culture

    Localization of intracellular NTHi. BEAS-2B cells were infected with NTHi strain ATCC 19418 for 2 hours. After killing extracellular bacteria with gentamicin, epithelial cells and bacteria were stained with several fluorescent dyes and Hoechst (blue). a Representative fluorescent micrographs of intracellular NTHi (blue) and epithelial cells stained with early endosomal marker (EEA-1, red). White arrows show EEA-1-positive regions, and white arrowheads show NTHi. For a detail of merged image, see a top-right inset. b Representative fluorescence micrographs of intracellular NTHi (blue) and epithelial cells stained with late endosomal marker (LAMP-1, purple). White arrows show LAMP-1-positive regions, and white arrowheads show NTHi. For a detail of merged image, see a top-right inset. c Representative fluorescent micrographs of intracellular NTHi and epithelial cells stained with LIVE/DEAD® (green) and cells were stained with acidic lysosomal dye (LysoTracker® Red). White arrows show LysoTracker® Red-positive acidic organelles, and white arrowheads show NTHi. Fluorescent micrographs were taken at 2,000× magnification. White bars represent 5 μm. Another strain (HUSM 0481) was also tested and similar results were obtained
    Figure Legend Snippet: Localization of intracellular NTHi. BEAS-2B cells were infected with NTHi strain ATCC 19418 for 2 hours. After killing extracellular bacteria with gentamicin, epithelial cells and bacteria were stained with several fluorescent dyes and Hoechst (blue). a Representative fluorescent micrographs of intracellular NTHi (blue) and epithelial cells stained with early endosomal marker (EEA-1, red). White arrows show EEA-1-positive regions, and white arrowheads show NTHi. For a detail of merged image, see a top-right inset. b Representative fluorescence micrographs of intracellular NTHi (blue) and epithelial cells stained with late endosomal marker (LAMP-1, purple). White arrows show LAMP-1-positive regions, and white arrowheads show NTHi. For a detail of merged image, see a top-right inset. c Representative fluorescent micrographs of intracellular NTHi and epithelial cells stained with LIVE/DEAD® (green) and cells were stained with acidic lysosomal dye (LysoTracker® Red). White arrows show LysoTracker® Red-positive acidic organelles, and white arrowheads show NTHi. Fluorescent micrographs were taken at 2,000× magnification. White bars represent 5 μm. Another strain (HUSM 0481) was also tested and similar results were obtained

    Techniques Used: Infection, Staining, Marker, Fluorescence

    NTHi binding of immobilized vitronectin in the absence or presence of heparin. Human plasma vitronectin was bound to glass-bottomed dishes. NTHi was incubated on the plate-bound vitronectin for 30 minutes, and then the dishes were washed three times. In some experiments, plate-bound vitronectin was pretreated with heparin before incubation with NTHi. NTHi was stained with LIVE/DEAD®. Live NTHi is stained green. BSA was used as a negative control. Representative fluorescent micrographs show that both NTHi strains (ATCC 19418 ( a ) and HUSM 0481 ( b )) attached to plate-bound vitronectin and that this attachment is blocked by heparin. White bars represent 10 μm. Summaries of the numbers of attached NTHi (per field at 1,000× magnification) are shown in ( c ) for ATCC 19418 and ( d ) for HUSM 0481. Error bars represent SEM in three independent experiments that gave similar results. ** p < 0.01
    Figure Legend Snippet: NTHi binding of immobilized vitronectin in the absence or presence of heparin. Human plasma vitronectin was bound to glass-bottomed dishes. NTHi was incubated on the plate-bound vitronectin for 30 minutes, and then the dishes were washed three times. In some experiments, plate-bound vitronectin was pretreated with heparin before incubation with NTHi. NTHi was stained with LIVE/DEAD®. Live NTHi is stained green. BSA was used as a negative control. Representative fluorescent micrographs show that both NTHi strains (ATCC 19418 ( a ) and HUSM 0481 ( b )) attached to plate-bound vitronectin and that this attachment is blocked by heparin. White bars represent 10 μm. Summaries of the numbers of attached NTHi (per field at 1,000× magnification) are shown in ( c ) for ATCC 19418 and ( d ) for HUSM 0481. Error bars represent SEM in three independent experiments that gave similar results. ** p < 0.01

    Techniques Used: Binding Assay, Incubation, Staining, Negative Control

    Intracellular invasion of NTHi in the presence of heparin or RGD peptide. BEAS-2B cells were infected for 2 hours with one of the two NTHi strains (ATCC 19418 or HUSM 0481), E. coli (a negative control), or L. monocytogenes (a positive control). In some experiments, cells were pretreated with heparin or RGD-peptide. a After killing extracellular bacteria with gentamicin, epithelial cells and bacteria were stained with LIVE/DEAD®. The percentages of BEAS-2B cells invaded by each type of bacteria are shown. b After killing extracellular bacteria with gentamicin and lysing the BEAS-2B cells, the bacteria were cultured overnight. The number of colonies was counted and the percentages of CFU after gentamicin treatment of cells per input CFU were shown. Error bars represent SEM in three independent experiments that gave similar results. * p < 0.05 and ** p < 0.01
    Figure Legend Snippet: Intracellular invasion of NTHi in the presence of heparin or RGD peptide. BEAS-2B cells were infected for 2 hours with one of the two NTHi strains (ATCC 19418 or HUSM 0481), E. coli (a negative control), or L. monocytogenes (a positive control). In some experiments, cells were pretreated with heparin or RGD-peptide. a After killing extracellular bacteria with gentamicin, epithelial cells and bacteria were stained with LIVE/DEAD®. The percentages of BEAS-2B cells invaded by each type of bacteria are shown. b After killing extracellular bacteria with gentamicin and lysing the BEAS-2B cells, the bacteria were cultured overnight. The number of colonies was counted and the percentages of CFU after gentamicin treatment of cells per input CFU were shown. Error bars represent SEM in three independent experiments that gave similar results. * p < 0.05 and ** p < 0.01

    Techniques Used: Infection, Negative Control, Positive Control, Staining, Cell Culture

    Binding of NTHi to immobilized vitronectin in the presence of protein-E peptide. Human plasma vitronectin was bound to glass-bottomed dishes. NTHi (HUSM 0481) was incubated on the plate-bound vitronectin for 30 minutes, and then the dishes were washed three times. In some experiments, plate-bound vitronectin was pretreated with protein-E peptide (PE 84–108 ) before incubation with NTHi. BSA was used as a negative control. NTHi was stained with LIVE/DEAD®, and viable NTHi is stained green. a Representative fluorescent micrographs of NTHi incubated on plate-bound vitronectin that was untreated or pretreated with PE 84–108 . White bars represent 10 μm. b The number of attached NTHi per field at 1,000 × magnification that was pretreated with increasing concentrations of PE 84–108 . Error bars represent SEM in three independent experiments that gave similar results. ** p < 0.01
    Figure Legend Snippet: Binding of NTHi to immobilized vitronectin in the presence of protein-E peptide. Human plasma vitronectin was bound to glass-bottomed dishes. NTHi (HUSM 0481) was incubated on the plate-bound vitronectin for 30 minutes, and then the dishes were washed three times. In some experiments, plate-bound vitronectin was pretreated with protein-E peptide (PE 84–108 ) before incubation with NTHi. BSA was used as a negative control. NTHi was stained with LIVE/DEAD®, and viable NTHi is stained green. a Representative fluorescent micrographs of NTHi incubated on plate-bound vitronectin that was untreated or pretreated with PE 84–108 . White bars represent 10 μm. b The number of attached NTHi per field at 1,000 × magnification that was pretreated with increasing concentrations of PE 84–108 . Error bars represent SEM in three independent experiments that gave similar results. ** p < 0.01

    Techniques Used: Binding Assay, Incubation, Negative Control, Staining

    Intracellular invasion of NTHi in the absence or presence of protein-E. BEAS-2B cells were infected for 2 hours with one of the two NTHi strains (ATCC 19418 or HUSM 0481) with or without pretreatment with PE 84–108 peptide. a After killing extracellular bacteria with gentamicin, epithelial cells and bacteria were stained with LIVE/DEAD®. The percentages of BEAS-2B cells invaded by each NTHi strain are shown. b After killing extracellular bacteria with gentamicin, cells were lysed and the bacteria were cultured overnight. The number of colonies was counted and the percentages of CFU after gentamicin treatment of cells per input CFU were shown. Error bars represent SEM in three independent experiments that gave similar results. * p < 0.05 and ** p < 0.01
    Figure Legend Snippet: Intracellular invasion of NTHi in the absence or presence of protein-E. BEAS-2B cells were infected for 2 hours with one of the two NTHi strains (ATCC 19418 or HUSM 0481) with or without pretreatment with PE 84–108 peptide. a After killing extracellular bacteria with gentamicin, epithelial cells and bacteria were stained with LIVE/DEAD®. The percentages of BEAS-2B cells invaded by each NTHi strain are shown. b After killing extracellular bacteria with gentamicin, cells were lysed and the bacteria were cultured overnight. The number of colonies was counted and the percentages of CFU after gentamicin treatment of cells per input CFU were shown. Error bars represent SEM in three independent experiments that gave similar results. * p < 0.05 and ** p < 0.01

    Techniques Used: Infection, Staining, Cell Culture

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    ATCC nthi strains
    <t>NTHi</t> penetration of bronchial epithelial <t>cells.</t> <t>BEAS-2B</t> cells were infected with one of two NTHi strains (ATCC 19418 or HUSM 0481), E. coli (a negative control), or L. monocytogenes (a positive control) for 2 hours. After killing extracellular bacteria with gentamicin, epithelial cells and bacteria were stained with LIVE/DEAD® and Hoechst. a Representative fluorescence micrographs of L. monocytogenes , and NTHi strain ATCC 19418. White arrows show viable intracellular bacteria stained green. White bars represent 5 μm. Fluorescent micrographs were taken at 2,000× magnification. b The percentages of epithelial cells invaded by bacteria. c After killing extracellular bacteria with gentamicin, cells were lysed and the bacteria were cultured overnight. The number of colonies was counted and the percentages of CFU after gentamicin treatment per input CFU were shown. Error bars represent SEM in three independent experiments that gave similar results. * p < 0.05 and ** p < 0.01
    Nthi Strains, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nthi strains/product/ATCC
    Average 99 stars, based on 1 article reviews
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    ATCC nthi 19418
    <t>NTHi</t> penetration of bronchial epithelial <t>cells.</t> <t>BEAS-2B</t> cells were infected with one of two NTHi strains (ATCC 19418 or HUSM 0481), E. coli (a negative control), or L. monocytogenes (a positive control) for 2 hours. After killing extracellular bacteria with gentamicin, epithelial cells and bacteria were stained with LIVE/DEAD® and Hoechst. a Representative fluorescence micrographs of L. monocytogenes , and NTHi strain ATCC 19418. White arrows show viable intracellular bacteria stained green. White bars represent 5 μm. Fluorescent micrographs were taken at 2,000× magnification. b The percentages of epithelial cells invaded by bacteria. c After killing extracellular bacteria with gentamicin, cells were lysed and the bacteria were cultured overnight. The number of colonies was counted and the percentages of CFU after gentamicin treatment per input CFU were shown. Error bars represent SEM in three independent experiments that gave similar results. * p < 0.05 and ** p < 0.01
    Nthi 19418, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC nthi strains a atcc 19418
    NTHi penetration of bronchial epithelial cells. BEAS-2B cells were infected with one of two NTHi strains (ATCC 19418 or HUSM 0481), E. coli (a negative control), or L. monocytogenes (a positive control) for 2 hours. After killing extracellular bacteria with gentamicin, epithelial cells and bacteria were stained with LIVE/DEAD® and Hoechst. a Representative fluorescence micrographs of L. monocytogenes , and NTHi strain ATCC 19418. White arrows show viable intracellular bacteria stained green. White bars represent 5 μm. Fluorescent micrographs were taken at 2,000× magnification. b The percentages of epithelial cells invaded by bacteria. c After killing extracellular bacteria with gentamicin, cells were lysed and the bacteria were cultured overnight. The number of colonies was counted and the percentages of CFU after gentamicin treatment per input CFU were shown. Error bars represent SEM in three independent experiments that gave similar results. * p < 0.05 and ** p < 0.01
    Nthi Strains A Atcc 19418, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nthi strains a atcc 19418/product/ATCC
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    ATCC nthi strain atcc 19418
    NTHi penetration of bronchial epithelial cells. BEAS-2B cells were infected with one of two NTHi strains (ATCC 19418 or HUSM 0481), E. coli (a negative control), or L. monocytogenes (a positive control) for 2 hours. After killing extracellular bacteria with gentamicin, epithelial cells and bacteria were stained with LIVE/DEAD® and Hoechst. a Representative fluorescence micrographs of L. monocytogenes , and NTHi strain ATCC 19418. White arrows show viable intracellular bacteria stained green. White bars represent 5 μm. Fluorescent micrographs were taken at 2,000× magnification. b The percentages of epithelial cells invaded by bacteria. c After killing extracellular bacteria with gentamicin, cells were lysed and the bacteria were cultured overnight. The number of colonies was counted and the percentages of CFU after gentamicin treatment per input CFU were shown. Error bars represent SEM in three independent experiments that gave similar results. * p < 0.05 and ** p < 0.01
    Nthi Strain Atcc 19418, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    nthi  (ATCC)
    99
    ATCC nthi
    <t>NTHi</t> penetration of bronchial epithelial cells. BEAS-2B cells were infected with one of two NTHi <t>strains</t> <t>(ATCC</t> 19418 or HUSM 0481), E. coli (a negative control), or L. monocytogenes (a positive control) for 2 hours. After killing extracellular bacteria with gentamicin, epithelial cells and bacteria were stained with LIVE/DEAD® and Hoechst. a Representative fluorescence micrographs of L. monocytogenes , and NTHi strain ATCC 19418. White arrows show viable intracellular bacteria stained green. White bars represent 5 μm. Fluorescent micrographs were taken at 2,000× magnification. b The percentages of epithelial cells invaded by bacteria. c After killing extracellular bacteria with gentamicin, cells were lysed and the bacteria were cultured overnight. The number of colonies was counted and the percentages of CFU after gentamicin treatment per input CFU were shown. Error bars represent SEM in three independent experiments that gave similar results. * p < 0.05 and ** p < 0.01
    Nthi, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nthi/product/ATCC
    Average 99 stars, based on 1 article reviews
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    NTHi penetration of bronchial epithelial cells. BEAS-2B cells were infected with one of two NTHi strains (ATCC 19418 or HUSM 0481), E. coli (a negative control), or L. monocytogenes (a positive control) for 2 hours. After killing extracellular bacteria with gentamicin, epithelial cells and bacteria were stained with LIVE/DEAD® and Hoechst. a Representative fluorescence micrographs of L. monocytogenes , and NTHi strain ATCC 19418. White arrows show viable intracellular bacteria stained green. White bars represent 5 μm. Fluorescent micrographs were taken at 2,000× magnification. b The percentages of epithelial cells invaded by bacteria. c After killing extracellular bacteria with gentamicin, cells were lysed and the bacteria were cultured overnight. The number of colonies was counted and the percentages of CFU after gentamicin treatment per input CFU were shown. Error bars represent SEM in three independent experiments that gave similar results. * p < 0.05 and ** p < 0.01

    Journal: BMC Microbiology

    Article Title: Nontypeable Haemophilus influenzae exploits the interaction between protein-E and vitronectin for the adherence and invasion to bronchial epithelial cells

    doi: 10.1186/s12866-015-0600-8

    Figure Lengend Snippet: NTHi penetration of bronchial epithelial cells. BEAS-2B cells were infected with one of two NTHi strains (ATCC 19418 or HUSM 0481), E. coli (a negative control), or L. monocytogenes (a positive control) for 2 hours. After killing extracellular bacteria with gentamicin, epithelial cells and bacteria were stained with LIVE/DEAD® and Hoechst. a Representative fluorescence micrographs of L. monocytogenes , and NTHi strain ATCC 19418. White arrows show viable intracellular bacteria stained green. White bars represent 5 μm. Fluorescent micrographs were taken at 2,000× magnification. b The percentages of epithelial cells invaded by bacteria. c After killing extracellular bacteria with gentamicin, cells were lysed and the bacteria were cultured overnight. The number of colonies was counted and the percentages of CFU after gentamicin treatment per input CFU were shown. Error bars represent SEM in three independent experiments that gave similar results. * p < 0.05 and ** p < 0.01

    Article Snippet: BEAS-2B cells were infected for 2 hours with one of the two NTHi strains (ATCC 19418 or HUSM 0481), E. coli (a negative control), or L. monocytogenes (a positive control).

    Techniques: Infection, Negative Control, Positive Control, Staining, Fluorescence, Cell Culture

    Localization of intracellular NTHi. BEAS-2B cells were infected with NTHi strain ATCC 19418 for 2 hours. After killing extracellular bacteria with gentamicin, epithelial cells and bacteria were stained with several fluorescent dyes and Hoechst (blue). a Representative fluorescent micrographs of intracellular NTHi (blue) and epithelial cells stained with early endosomal marker (EEA-1, red). White arrows show EEA-1-positive regions, and white arrowheads show NTHi. For a detail of merged image, see a top-right inset. b Representative fluorescence micrographs of intracellular NTHi (blue) and epithelial cells stained with late endosomal marker (LAMP-1, purple). White arrows show LAMP-1-positive regions, and white arrowheads show NTHi. For a detail of merged image, see a top-right inset. c Representative fluorescent micrographs of intracellular NTHi and epithelial cells stained with LIVE/DEAD® (green) and cells were stained with acidic lysosomal dye (LysoTracker® Red). White arrows show LysoTracker® Red-positive acidic organelles, and white arrowheads show NTHi. Fluorescent micrographs were taken at 2,000× magnification. White bars represent 5 μm. Another strain (HUSM 0481) was also tested and similar results were obtained

    Journal: BMC Microbiology

    Article Title: Nontypeable Haemophilus influenzae exploits the interaction between protein-E and vitronectin for the adherence and invasion to bronchial epithelial cells

    doi: 10.1186/s12866-015-0600-8

    Figure Lengend Snippet: Localization of intracellular NTHi. BEAS-2B cells were infected with NTHi strain ATCC 19418 for 2 hours. After killing extracellular bacteria with gentamicin, epithelial cells and bacteria were stained with several fluorescent dyes and Hoechst (blue). a Representative fluorescent micrographs of intracellular NTHi (blue) and epithelial cells stained with early endosomal marker (EEA-1, red). White arrows show EEA-1-positive regions, and white arrowheads show NTHi. For a detail of merged image, see a top-right inset. b Representative fluorescence micrographs of intracellular NTHi (blue) and epithelial cells stained with late endosomal marker (LAMP-1, purple). White arrows show LAMP-1-positive regions, and white arrowheads show NTHi. For a detail of merged image, see a top-right inset. c Representative fluorescent micrographs of intracellular NTHi and epithelial cells stained with LIVE/DEAD® (green) and cells were stained with acidic lysosomal dye (LysoTracker® Red). White arrows show LysoTracker® Red-positive acidic organelles, and white arrowheads show NTHi. Fluorescent micrographs were taken at 2,000× magnification. White bars represent 5 μm. Another strain (HUSM 0481) was also tested and similar results were obtained

    Article Snippet: BEAS-2B cells were infected for 2 hours with one of the two NTHi strains (ATCC 19418 or HUSM 0481), E. coli (a negative control), or L. monocytogenes (a positive control).

    Techniques: Infection, Staining, Marker, Fluorescence

    NTHi binding of immobilized vitronectin in the absence or presence of heparin. Human plasma vitronectin was bound to glass-bottomed dishes. NTHi was incubated on the plate-bound vitronectin for 30 minutes, and then the dishes were washed three times. In some experiments, plate-bound vitronectin was pretreated with heparin before incubation with NTHi. NTHi was stained with LIVE/DEAD®. Live NTHi is stained green. BSA was used as a negative control. Representative fluorescent micrographs show that both NTHi strains (ATCC 19418 ( a ) and HUSM 0481 ( b )) attached to plate-bound vitronectin and that this attachment is blocked by heparin. White bars represent 10 μm. Summaries of the numbers of attached NTHi (per field at 1,000× magnification) are shown in ( c ) for ATCC 19418 and ( d ) for HUSM 0481. Error bars represent SEM in three independent experiments that gave similar results. ** p < 0.01

    Journal: BMC Microbiology

    Article Title: Nontypeable Haemophilus influenzae exploits the interaction between protein-E and vitronectin for the adherence and invasion to bronchial epithelial cells

    doi: 10.1186/s12866-015-0600-8

    Figure Lengend Snippet: NTHi binding of immobilized vitronectin in the absence or presence of heparin. Human plasma vitronectin was bound to glass-bottomed dishes. NTHi was incubated on the plate-bound vitronectin for 30 minutes, and then the dishes were washed three times. In some experiments, plate-bound vitronectin was pretreated with heparin before incubation with NTHi. NTHi was stained with LIVE/DEAD®. Live NTHi is stained green. BSA was used as a negative control. Representative fluorescent micrographs show that both NTHi strains (ATCC 19418 ( a ) and HUSM 0481 ( b )) attached to plate-bound vitronectin and that this attachment is blocked by heparin. White bars represent 10 μm. Summaries of the numbers of attached NTHi (per field at 1,000× magnification) are shown in ( c ) for ATCC 19418 and ( d ) for HUSM 0481. Error bars represent SEM in three independent experiments that gave similar results. ** p < 0.01

    Article Snippet: BEAS-2B cells were infected for 2 hours with one of the two NTHi strains (ATCC 19418 or HUSM 0481), E. coli (a negative control), or L. monocytogenes (a positive control).

    Techniques: Binding Assay, Incubation, Staining, Negative Control

    Intracellular invasion of NTHi in the presence of heparin or RGD peptide. BEAS-2B cells were infected for 2 hours with one of the two NTHi strains (ATCC 19418 or HUSM 0481), E. coli (a negative control), or L. monocytogenes (a positive control). In some experiments, cells were pretreated with heparin or RGD-peptide. a After killing extracellular bacteria with gentamicin, epithelial cells and bacteria were stained with LIVE/DEAD®. The percentages of BEAS-2B cells invaded by each type of bacteria are shown. b After killing extracellular bacteria with gentamicin and lysing the BEAS-2B cells, the bacteria were cultured overnight. The number of colonies was counted and the percentages of CFU after gentamicin treatment of cells per input CFU were shown. Error bars represent SEM in three independent experiments that gave similar results. * p < 0.05 and ** p < 0.01

    Journal: BMC Microbiology

    Article Title: Nontypeable Haemophilus influenzae exploits the interaction between protein-E and vitronectin for the adherence and invasion to bronchial epithelial cells

    doi: 10.1186/s12866-015-0600-8

    Figure Lengend Snippet: Intracellular invasion of NTHi in the presence of heparin or RGD peptide. BEAS-2B cells were infected for 2 hours with one of the two NTHi strains (ATCC 19418 or HUSM 0481), E. coli (a negative control), or L. monocytogenes (a positive control). In some experiments, cells were pretreated with heparin or RGD-peptide. a After killing extracellular bacteria with gentamicin, epithelial cells and bacteria were stained with LIVE/DEAD®. The percentages of BEAS-2B cells invaded by each type of bacteria are shown. b After killing extracellular bacteria with gentamicin and lysing the BEAS-2B cells, the bacteria were cultured overnight. The number of colonies was counted and the percentages of CFU after gentamicin treatment of cells per input CFU were shown. Error bars represent SEM in three independent experiments that gave similar results. * p < 0.05 and ** p < 0.01

    Article Snippet: BEAS-2B cells were infected for 2 hours with one of the two NTHi strains (ATCC 19418 or HUSM 0481), E. coli (a negative control), or L. monocytogenes (a positive control).

    Techniques: Infection, Negative Control, Positive Control, Staining, Cell Culture

    Binding of NTHi to immobilized vitronectin in the presence of protein-E peptide. Human plasma vitronectin was bound to glass-bottomed dishes. NTHi (HUSM 0481) was incubated on the plate-bound vitronectin for 30 minutes, and then the dishes were washed three times. In some experiments, plate-bound vitronectin was pretreated with protein-E peptide (PE 84–108 ) before incubation with NTHi. BSA was used as a negative control. NTHi was stained with LIVE/DEAD®, and viable NTHi is stained green. a Representative fluorescent micrographs of NTHi incubated on plate-bound vitronectin that was untreated or pretreated with PE 84–108 . White bars represent 10 μm. b The number of attached NTHi per field at 1,000 × magnification that was pretreated with increasing concentrations of PE 84–108 . Error bars represent SEM in three independent experiments that gave similar results. ** p < 0.01

    Journal: BMC Microbiology

    Article Title: Nontypeable Haemophilus influenzae exploits the interaction between protein-E and vitronectin for the adherence and invasion to bronchial epithelial cells

    doi: 10.1186/s12866-015-0600-8

    Figure Lengend Snippet: Binding of NTHi to immobilized vitronectin in the presence of protein-E peptide. Human plasma vitronectin was bound to glass-bottomed dishes. NTHi (HUSM 0481) was incubated on the plate-bound vitronectin for 30 minutes, and then the dishes were washed three times. In some experiments, plate-bound vitronectin was pretreated with protein-E peptide (PE 84–108 ) before incubation with NTHi. BSA was used as a negative control. NTHi was stained with LIVE/DEAD®, and viable NTHi is stained green. a Representative fluorescent micrographs of NTHi incubated on plate-bound vitronectin that was untreated or pretreated with PE 84–108 . White bars represent 10 μm. b The number of attached NTHi per field at 1,000 × magnification that was pretreated with increasing concentrations of PE 84–108 . Error bars represent SEM in three independent experiments that gave similar results. ** p < 0.01

    Article Snippet: BEAS-2B cells were infected for 2 hours with one of the two NTHi strains (ATCC 19418 or HUSM 0481), E. coli (a negative control), or L. monocytogenes (a positive control).

    Techniques: Binding Assay, Incubation, Negative Control, Staining

    Intracellular invasion of NTHi in the absence or presence of protein-E. BEAS-2B cells were infected for 2 hours with one of the two NTHi strains (ATCC 19418 or HUSM 0481) with or without pretreatment with PE 84–108 peptide. a After killing extracellular bacteria with gentamicin, epithelial cells and bacteria were stained with LIVE/DEAD®. The percentages of BEAS-2B cells invaded by each NTHi strain are shown. b After killing extracellular bacteria with gentamicin, cells were lysed and the bacteria were cultured overnight. The number of colonies was counted and the percentages of CFU after gentamicin treatment of cells per input CFU were shown. Error bars represent SEM in three independent experiments that gave similar results. * p < 0.05 and ** p < 0.01

    Journal: BMC Microbiology

    Article Title: Nontypeable Haemophilus influenzae exploits the interaction between protein-E and vitronectin for the adherence and invasion to bronchial epithelial cells

    doi: 10.1186/s12866-015-0600-8

    Figure Lengend Snippet: Intracellular invasion of NTHi in the absence or presence of protein-E. BEAS-2B cells were infected for 2 hours with one of the two NTHi strains (ATCC 19418 or HUSM 0481) with or without pretreatment with PE 84–108 peptide. a After killing extracellular bacteria with gentamicin, epithelial cells and bacteria were stained with LIVE/DEAD®. The percentages of BEAS-2B cells invaded by each NTHi strain are shown. b After killing extracellular bacteria with gentamicin, cells were lysed and the bacteria were cultured overnight. The number of colonies was counted and the percentages of CFU after gentamicin treatment of cells per input CFU were shown. Error bars represent SEM in three independent experiments that gave similar results. * p < 0.05 and ** p < 0.01

    Article Snippet: BEAS-2B cells were infected for 2 hours with one of the two NTHi strains (ATCC 19418 or HUSM 0481), E. coli (a negative control), or L. monocytogenes (a positive control).

    Techniques: Infection, Staining, Cell Culture

    NTHi penetration of bronchial epithelial cells. BEAS-2B cells were infected with one of two NTHi strains (ATCC 19418 or HUSM 0481), E. coli (a negative control), or L. monocytogenes (a positive control) for 2 hours. After killing extracellular bacteria with gentamicin, epithelial cells and bacteria were stained with LIVE/DEAD® and Hoechst. a Representative fluorescence micrographs of L. monocytogenes , and NTHi strain ATCC 19418. White arrows show viable intracellular bacteria stained green. White bars represent 5 μm. Fluorescent micrographs were taken at 2,000× magnification. b The percentages of epithelial cells invaded by bacteria. c After killing extracellular bacteria with gentamicin, cells were lysed and the bacteria were cultured overnight. The number of colonies was counted and the percentages of CFU after gentamicin treatment per input CFU were shown. Error bars represent SEM in three independent experiments that gave similar results. * p < 0.05 and ** p < 0.01

    Journal: BMC Microbiology

    Article Title: Nontypeable Haemophilus influenzae exploits the interaction between protein-E and vitronectin for the adherence and invasion to bronchial epithelial cells

    doi: 10.1186/s12866-015-0600-8

    Figure Lengend Snippet: NTHi penetration of bronchial epithelial cells. BEAS-2B cells were infected with one of two NTHi strains (ATCC 19418 or HUSM 0481), E. coli (a negative control), or L. monocytogenes (a positive control) for 2 hours. After killing extracellular bacteria with gentamicin, epithelial cells and bacteria were stained with LIVE/DEAD® and Hoechst. a Representative fluorescence micrographs of L. monocytogenes , and NTHi strain ATCC 19418. White arrows show viable intracellular bacteria stained green. White bars represent 5 μm. Fluorescent micrographs were taken at 2,000× magnification. b The percentages of epithelial cells invaded by bacteria. c After killing extracellular bacteria with gentamicin, cells were lysed and the bacteria were cultured overnight. The number of colonies was counted and the percentages of CFU after gentamicin treatment per input CFU were shown. Error bars represent SEM in three independent experiments that gave similar results. * p < 0.05 and ** p < 0.01

    Article Snippet: These cells were infected for 2 hours with one of the two NTHi strains ((A) ATCC 19418 or (B) HUSM 0481).

    Techniques: Infection, Negative Control, Positive Control, Staining, Fluorescence, Cell Culture

    Localization of intracellular NTHi. BEAS-2B cells were infected with NTHi strain ATCC 19418 for 2 hours. After killing extracellular bacteria with gentamicin, epithelial cells and bacteria were stained with several fluorescent dyes and Hoechst (blue). a Representative fluorescent micrographs of intracellular NTHi (blue) and epithelial cells stained with early endosomal marker (EEA-1, red). White arrows show EEA-1-positive regions, and white arrowheads show NTHi. For a detail of merged image, see a top-right inset. b Representative fluorescence micrographs of intracellular NTHi (blue) and epithelial cells stained with late endosomal marker (LAMP-1, purple). White arrows show LAMP-1-positive regions, and white arrowheads show NTHi. For a detail of merged image, see a top-right inset. c Representative fluorescent micrographs of intracellular NTHi and epithelial cells stained with LIVE/DEAD® (green) and cells were stained with acidic lysosomal dye (LysoTracker® Red). White arrows show LysoTracker® Red-positive acidic organelles, and white arrowheads show NTHi. Fluorescent micrographs were taken at 2,000× magnification. White bars represent 5 μm. Another strain (HUSM 0481) was also tested and similar results were obtained

    Journal: BMC Microbiology

    Article Title: Nontypeable Haemophilus influenzae exploits the interaction between protein-E and vitronectin for the adherence and invasion to bronchial epithelial cells

    doi: 10.1186/s12866-015-0600-8

    Figure Lengend Snippet: Localization of intracellular NTHi. BEAS-2B cells were infected with NTHi strain ATCC 19418 for 2 hours. After killing extracellular bacteria with gentamicin, epithelial cells and bacteria were stained with several fluorescent dyes and Hoechst (blue). a Representative fluorescent micrographs of intracellular NTHi (blue) and epithelial cells stained with early endosomal marker (EEA-1, red). White arrows show EEA-1-positive regions, and white arrowheads show NTHi. For a detail of merged image, see a top-right inset. b Representative fluorescence micrographs of intracellular NTHi (blue) and epithelial cells stained with late endosomal marker (LAMP-1, purple). White arrows show LAMP-1-positive regions, and white arrowheads show NTHi. For a detail of merged image, see a top-right inset. c Representative fluorescent micrographs of intracellular NTHi and epithelial cells stained with LIVE/DEAD® (green) and cells were stained with acidic lysosomal dye (LysoTracker® Red). White arrows show LysoTracker® Red-positive acidic organelles, and white arrowheads show NTHi. Fluorescent micrographs were taken at 2,000× magnification. White bars represent 5 μm. Another strain (HUSM 0481) was also tested and similar results were obtained

    Article Snippet: These cells were infected for 2 hours with one of the two NTHi strains ((A) ATCC 19418 or (B) HUSM 0481).

    Techniques: Infection, Staining, Marker, Fluorescence

    NTHi binding of immobilized vitronectin in the absence or presence of heparin. Human plasma vitronectin was bound to glass-bottomed dishes. NTHi was incubated on the plate-bound vitronectin for 30 minutes, and then the dishes were washed three times. In some experiments, plate-bound vitronectin was pretreated with heparin before incubation with NTHi. NTHi was stained with LIVE/DEAD®. Live NTHi is stained green. BSA was used as a negative control. Representative fluorescent micrographs show that both NTHi strains (ATCC 19418 ( a ) and HUSM 0481 ( b )) attached to plate-bound vitronectin and that this attachment is blocked by heparin. White bars represent 10 μm. Summaries of the numbers of attached NTHi (per field at 1,000× magnification) are shown in ( c ) for ATCC 19418 and ( d ) for HUSM 0481. Error bars represent SEM in three independent experiments that gave similar results. ** p < 0.01

    Journal: BMC Microbiology

    Article Title: Nontypeable Haemophilus influenzae exploits the interaction between protein-E and vitronectin for the adherence and invasion to bronchial epithelial cells

    doi: 10.1186/s12866-015-0600-8

    Figure Lengend Snippet: NTHi binding of immobilized vitronectin in the absence or presence of heparin. Human plasma vitronectin was bound to glass-bottomed dishes. NTHi was incubated on the plate-bound vitronectin for 30 minutes, and then the dishes were washed three times. In some experiments, plate-bound vitronectin was pretreated with heparin before incubation with NTHi. NTHi was stained with LIVE/DEAD®. Live NTHi is stained green. BSA was used as a negative control. Representative fluorescent micrographs show that both NTHi strains (ATCC 19418 ( a ) and HUSM 0481 ( b )) attached to plate-bound vitronectin and that this attachment is blocked by heparin. White bars represent 10 μm. Summaries of the numbers of attached NTHi (per field at 1,000× magnification) are shown in ( c ) for ATCC 19418 and ( d ) for HUSM 0481. Error bars represent SEM in three independent experiments that gave similar results. ** p < 0.01

    Article Snippet: These cells were infected for 2 hours with one of the two NTHi strains ((A) ATCC 19418 or (B) HUSM 0481).

    Techniques: Binding Assay, Incubation, Staining, Negative Control

    Intracellular invasion of NTHi in the presence of heparin or RGD peptide. BEAS-2B cells were infected for 2 hours with one of the two NTHi strains (ATCC 19418 or HUSM 0481), E. coli (a negative control), or L. monocytogenes (a positive control). In some experiments, cells were pretreated with heparin or RGD-peptide. a After killing extracellular bacteria with gentamicin, epithelial cells and bacteria were stained with LIVE/DEAD®. The percentages of BEAS-2B cells invaded by each type of bacteria are shown. b After killing extracellular bacteria with gentamicin and lysing the BEAS-2B cells, the bacteria were cultured overnight. The number of colonies was counted and the percentages of CFU after gentamicin treatment of cells per input CFU were shown. Error bars represent SEM in three independent experiments that gave similar results. * p < 0.05 and ** p < 0.01

    Journal: BMC Microbiology

    Article Title: Nontypeable Haemophilus influenzae exploits the interaction between protein-E and vitronectin for the adherence and invasion to bronchial epithelial cells

    doi: 10.1186/s12866-015-0600-8

    Figure Lengend Snippet: Intracellular invasion of NTHi in the presence of heparin or RGD peptide. BEAS-2B cells were infected for 2 hours with one of the two NTHi strains (ATCC 19418 or HUSM 0481), E. coli (a negative control), or L. monocytogenes (a positive control). In some experiments, cells were pretreated with heparin or RGD-peptide. a After killing extracellular bacteria with gentamicin, epithelial cells and bacteria were stained with LIVE/DEAD®. The percentages of BEAS-2B cells invaded by each type of bacteria are shown. b After killing extracellular bacteria with gentamicin and lysing the BEAS-2B cells, the bacteria were cultured overnight. The number of colonies was counted and the percentages of CFU after gentamicin treatment of cells per input CFU were shown. Error bars represent SEM in three independent experiments that gave similar results. * p < 0.05 and ** p < 0.01

    Article Snippet: These cells were infected for 2 hours with one of the two NTHi strains ((A) ATCC 19418 or (B) HUSM 0481).

    Techniques: Infection, Negative Control, Positive Control, Staining, Cell Culture

    Intracellular invasion of NTHi in the absence or presence of protein-E. BEAS-2B cells were infected for 2 hours with one of the two NTHi strains (ATCC 19418 or HUSM 0481) with or without pretreatment with PE 84–108 peptide. a After killing extracellular bacteria with gentamicin, epithelial cells and bacteria were stained with LIVE/DEAD®. The percentages of BEAS-2B cells invaded by each NTHi strain are shown. b After killing extracellular bacteria with gentamicin, cells were lysed and the bacteria were cultured overnight. The number of colonies was counted and the percentages of CFU after gentamicin treatment of cells per input CFU were shown. Error bars represent SEM in three independent experiments that gave similar results. * p < 0.05 and ** p < 0.01

    Journal: BMC Microbiology

    Article Title: Nontypeable Haemophilus influenzae exploits the interaction between protein-E and vitronectin for the adherence and invasion to bronchial epithelial cells

    doi: 10.1186/s12866-015-0600-8

    Figure Lengend Snippet: Intracellular invasion of NTHi in the absence or presence of protein-E. BEAS-2B cells were infected for 2 hours with one of the two NTHi strains (ATCC 19418 or HUSM 0481) with or without pretreatment with PE 84–108 peptide. a After killing extracellular bacteria with gentamicin, epithelial cells and bacteria were stained with LIVE/DEAD®. The percentages of BEAS-2B cells invaded by each NTHi strain are shown. b After killing extracellular bacteria with gentamicin, cells were lysed and the bacteria were cultured overnight. The number of colonies was counted and the percentages of CFU after gentamicin treatment of cells per input CFU were shown. Error bars represent SEM in three independent experiments that gave similar results. * p < 0.05 and ** p < 0.01

    Article Snippet: These cells were infected for 2 hours with one of the two NTHi strains ((A) ATCC 19418 or (B) HUSM 0481).

    Techniques: Infection, Staining, Cell Culture

    NTHi penetration of bronchial epithelial cells. BEAS-2B cells were infected with one of two NTHi strains (ATCC 19418 or HUSM 0481), E. coli (a negative control), or L. monocytogenes (a positive control) for 2 hours. After killing extracellular bacteria with gentamicin, epithelial cells and bacteria were stained with LIVE/DEAD® and Hoechst. a Representative fluorescence micrographs of L. monocytogenes , and NTHi strain ATCC 19418. White arrows show viable intracellular bacteria stained green. White bars represent 5 μm. Fluorescent micrographs were taken at 2,000× magnification. b The percentages of epithelial cells invaded by bacteria. c After killing extracellular bacteria with gentamicin, cells were lysed and the bacteria were cultured overnight. The number of colonies was counted and the percentages of CFU after gentamicin treatment per input CFU were shown. Error bars represent SEM in three independent experiments that gave similar results. * p < 0.05 and ** p < 0.01

    Journal: BMC Microbiology

    Article Title: Nontypeable Haemophilus influenzae exploits the interaction between protein-E and vitronectin for the adherence and invasion to bronchial epithelial cells

    doi: 10.1186/s12866-015-0600-8

    Figure Lengend Snippet: NTHi penetration of bronchial epithelial cells. BEAS-2B cells were infected with one of two NTHi strains (ATCC 19418 or HUSM 0481), E. coli (a negative control), or L. monocytogenes (a positive control) for 2 hours. After killing extracellular bacteria with gentamicin, epithelial cells and bacteria were stained with LIVE/DEAD® and Hoechst. a Representative fluorescence micrographs of L. monocytogenes , and NTHi strain ATCC 19418. White arrows show viable intracellular bacteria stained green. White bars represent 5 μm. Fluorescent micrographs were taken at 2,000× magnification. b The percentages of epithelial cells invaded by bacteria. c After killing extracellular bacteria with gentamicin, cells were lysed and the bacteria were cultured overnight. The number of colonies was counted and the percentages of CFU after gentamicin treatment per input CFU were shown. Error bars represent SEM in three independent experiments that gave similar results. * p < 0.05 and ** p < 0.01

    Article Snippet: The other was a commercially available NTHi strain ATCC 19418 (American Type Culture Collection (ATCC), Manassas, VA).

    Techniques: Infection, Negative Control, Positive Control, Staining, Fluorescence, Cell Culture

    Localization of intracellular NTHi. BEAS-2B cells were infected with NTHi strain ATCC 19418 for 2 hours. After killing extracellular bacteria with gentamicin, epithelial cells and bacteria were stained with several fluorescent dyes and Hoechst (blue). a Representative fluorescent micrographs of intracellular NTHi (blue) and epithelial cells stained with early endosomal marker (EEA-1, red). White arrows show EEA-1-positive regions, and white arrowheads show NTHi. For a detail of merged image, see a top-right inset. b Representative fluorescence micrographs of intracellular NTHi (blue) and epithelial cells stained with late endosomal marker (LAMP-1, purple). White arrows show LAMP-1-positive regions, and white arrowheads show NTHi. For a detail of merged image, see a top-right inset. c Representative fluorescent micrographs of intracellular NTHi and epithelial cells stained with LIVE/DEAD® (green) and cells were stained with acidic lysosomal dye (LysoTracker® Red). White arrows show LysoTracker® Red-positive acidic organelles, and white arrowheads show NTHi. Fluorescent micrographs were taken at 2,000× magnification. White bars represent 5 μm. Another strain (HUSM 0481) was also tested and similar results were obtained

    Journal: BMC Microbiology

    Article Title: Nontypeable Haemophilus influenzae exploits the interaction between protein-E and vitronectin for the adherence and invasion to bronchial epithelial cells

    doi: 10.1186/s12866-015-0600-8

    Figure Lengend Snippet: Localization of intracellular NTHi. BEAS-2B cells were infected with NTHi strain ATCC 19418 for 2 hours. After killing extracellular bacteria with gentamicin, epithelial cells and bacteria were stained with several fluorescent dyes and Hoechst (blue). a Representative fluorescent micrographs of intracellular NTHi (blue) and epithelial cells stained with early endosomal marker (EEA-1, red). White arrows show EEA-1-positive regions, and white arrowheads show NTHi. For a detail of merged image, see a top-right inset. b Representative fluorescence micrographs of intracellular NTHi (blue) and epithelial cells stained with late endosomal marker (LAMP-1, purple). White arrows show LAMP-1-positive regions, and white arrowheads show NTHi. For a detail of merged image, see a top-right inset. c Representative fluorescent micrographs of intracellular NTHi and epithelial cells stained with LIVE/DEAD® (green) and cells were stained with acidic lysosomal dye (LysoTracker® Red). White arrows show LysoTracker® Red-positive acidic organelles, and white arrowheads show NTHi. Fluorescent micrographs were taken at 2,000× magnification. White bars represent 5 μm. Another strain (HUSM 0481) was also tested and similar results were obtained

    Article Snippet: The other was a commercially available NTHi strain ATCC 19418 (American Type Culture Collection (ATCC), Manassas, VA).

    Techniques: Infection, Staining, Marker, Fluorescence

    NTHi binding of immobilized vitronectin in the absence or presence of heparin. Human plasma vitronectin was bound to glass-bottomed dishes. NTHi was incubated on the plate-bound vitronectin for 30 minutes, and then the dishes were washed three times. In some experiments, plate-bound vitronectin was pretreated with heparin before incubation with NTHi. NTHi was stained with LIVE/DEAD®. Live NTHi is stained green. BSA was used as a negative control. Representative fluorescent micrographs show that both NTHi strains (ATCC 19418 ( a ) and HUSM 0481 ( b )) attached to plate-bound vitronectin and that this attachment is blocked by heparin. White bars represent 10 μm. Summaries of the numbers of attached NTHi (per field at 1,000× magnification) are shown in ( c ) for ATCC 19418 and ( d ) for HUSM 0481. Error bars represent SEM in three independent experiments that gave similar results. ** p < 0.01

    Journal: BMC Microbiology

    Article Title: Nontypeable Haemophilus influenzae exploits the interaction between protein-E and vitronectin for the adherence and invasion to bronchial epithelial cells

    doi: 10.1186/s12866-015-0600-8

    Figure Lengend Snippet: NTHi binding of immobilized vitronectin in the absence or presence of heparin. Human plasma vitronectin was bound to glass-bottomed dishes. NTHi was incubated on the plate-bound vitronectin for 30 minutes, and then the dishes were washed three times. In some experiments, plate-bound vitronectin was pretreated with heparin before incubation with NTHi. NTHi was stained with LIVE/DEAD®. Live NTHi is stained green. BSA was used as a negative control. Representative fluorescent micrographs show that both NTHi strains (ATCC 19418 ( a ) and HUSM 0481 ( b )) attached to plate-bound vitronectin and that this attachment is blocked by heparin. White bars represent 10 μm. Summaries of the numbers of attached NTHi (per field at 1,000× magnification) are shown in ( c ) for ATCC 19418 and ( d ) for HUSM 0481. Error bars represent SEM in three independent experiments that gave similar results. ** p < 0.01

    Article Snippet: The other was a commercially available NTHi strain ATCC 19418 (American Type Culture Collection (ATCC), Manassas, VA).

    Techniques: Binding Assay, Incubation, Staining, Negative Control

    Intracellular invasion of NTHi in the presence of heparin or RGD peptide. BEAS-2B cells were infected for 2 hours with one of the two NTHi strains (ATCC 19418 or HUSM 0481), E. coli (a negative control), or L. monocytogenes (a positive control). In some experiments, cells were pretreated with heparin or RGD-peptide. a After killing extracellular bacteria with gentamicin, epithelial cells and bacteria were stained with LIVE/DEAD®. The percentages of BEAS-2B cells invaded by each type of bacteria are shown. b After killing extracellular bacteria with gentamicin and lysing the BEAS-2B cells, the bacteria were cultured overnight. The number of colonies was counted and the percentages of CFU after gentamicin treatment of cells per input CFU were shown. Error bars represent SEM in three independent experiments that gave similar results. * p < 0.05 and ** p < 0.01

    Journal: BMC Microbiology

    Article Title: Nontypeable Haemophilus influenzae exploits the interaction between protein-E and vitronectin for the adherence and invasion to bronchial epithelial cells

    doi: 10.1186/s12866-015-0600-8

    Figure Lengend Snippet: Intracellular invasion of NTHi in the presence of heparin or RGD peptide. BEAS-2B cells were infected for 2 hours with one of the two NTHi strains (ATCC 19418 or HUSM 0481), E. coli (a negative control), or L. monocytogenes (a positive control). In some experiments, cells were pretreated with heparin or RGD-peptide. a After killing extracellular bacteria with gentamicin, epithelial cells and bacteria were stained with LIVE/DEAD®. The percentages of BEAS-2B cells invaded by each type of bacteria are shown. b After killing extracellular bacteria with gentamicin and lysing the BEAS-2B cells, the bacteria were cultured overnight. The number of colonies was counted and the percentages of CFU after gentamicin treatment of cells per input CFU were shown. Error bars represent SEM in three independent experiments that gave similar results. * p < 0.05 and ** p < 0.01

    Article Snippet: The other was a commercially available NTHi strain ATCC 19418 (American Type Culture Collection (ATCC), Manassas, VA).

    Techniques: Infection, Negative Control, Positive Control, Staining, Cell Culture

    Intracellular invasion of NTHi in the absence or presence of protein-E. BEAS-2B cells were infected for 2 hours with one of the two NTHi strains (ATCC 19418 or HUSM 0481) with or without pretreatment with PE 84–108 peptide. a After killing extracellular bacteria with gentamicin, epithelial cells and bacteria were stained with LIVE/DEAD®. The percentages of BEAS-2B cells invaded by each NTHi strain are shown. b After killing extracellular bacteria with gentamicin, cells were lysed and the bacteria were cultured overnight. The number of colonies was counted and the percentages of CFU after gentamicin treatment of cells per input CFU were shown. Error bars represent SEM in three independent experiments that gave similar results. * p < 0.05 and ** p < 0.01

    Journal: BMC Microbiology

    Article Title: Nontypeable Haemophilus influenzae exploits the interaction between protein-E and vitronectin for the adherence and invasion to bronchial epithelial cells

    doi: 10.1186/s12866-015-0600-8

    Figure Lengend Snippet: Intracellular invasion of NTHi in the absence or presence of protein-E. BEAS-2B cells were infected for 2 hours with one of the two NTHi strains (ATCC 19418 or HUSM 0481) with or without pretreatment with PE 84–108 peptide. a After killing extracellular bacteria with gentamicin, epithelial cells and bacteria were stained with LIVE/DEAD®. The percentages of BEAS-2B cells invaded by each NTHi strain are shown. b After killing extracellular bacteria with gentamicin, cells were lysed and the bacteria were cultured overnight. The number of colonies was counted and the percentages of CFU after gentamicin treatment of cells per input CFU were shown. Error bars represent SEM in three independent experiments that gave similar results. * p < 0.05 and ** p < 0.01

    Article Snippet: The other was a commercially available NTHi strain ATCC 19418 (American Type Culture Collection (ATCC), Manassas, VA).

    Techniques: Infection, Staining, Cell Culture

    NTHi penetration of bronchial epithelial cells. BEAS-2B cells were infected with one of two NTHi strains (ATCC 19418 or HUSM 0481), E. coli (a negative control), or L. monocytogenes (a positive control) for 2 hours. After killing extracellular bacteria with gentamicin, epithelial cells and bacteria were stained with LIVE/DEAD® and Hoechst. a Representative fluorescence micrographs of L. monocytogenes , and NTHi strain ATCC 19418. White arrows show viable intracellular bacteria stained green. White bars represent 5 μm. Fluorescent micrographs were taken at 2,000× magnification. b The percentages of epithelial cells invaded by bacteria. c After killing extracellular bacteria with gentamicin, cells were lysed and the bacteria were cultured overnight. The number of colonies was counted and the percentages of CFU after gentamicin treatment per input CFU were shown. Error bars represent SEM in three independent experiments that gave similar results. * p < 0.05 and ** p < 0.01

    Journal: BMC Microbiology

    Article Title: Nontypeable Haemophilus influenzae exploits the interaction between protein-E and vitronectin for the adherence and invasion to bronchial epithelial cells

    doi: 10.1186/s12866-015-0600-8

    Figure Lengend Snippet: NTHi penetration of bronchial epithelial cells. BEAS-2B cells were infected with one of two NTHi strains (ATCC 19418 or HUSM 0481), E. coli (a negative control), or L. monocytogenes (a positive control) for 2 hours. After killing extracellular bacteria with gentamicin, epithelial cells and bacteria were stained with LIVE/DEAD® and Hoechst. a Representative fluorescence micrographs of L. monocytogenes , and NTHi strain ATCC 19418. White arrows show viable intracellular bacteria stained green. White bars represent 5 μm. Fluorescent micrographs were taken at 2,000× magnification. b The percentages of epithelial cells invaded by bacteria. c After killing extracellular bacteria with gentamicin, cells were lysed and the bacteria were cultured overnight. The number of colonies was counted and the percentages of CFU after gentamicin treatment per input CFU were shown. Error bars represent SEM in three independent experiments that gave similar results. * p < 0.05 and ** p < 0.01

    Article Snippet: The percentage of BEAS-2B cells invaded by each NTHi strain is shown in Fig. . Pretreatment with PE 84–108 significantly reduced the percentage of invaded cells by either strain of NTHi (Fig. , ATCC 19418: p < 0.001, HUSM 0481: p < 0.01).

    Techniques: Infection, Negative Control, Positive Control, Staining, Fluorescence, Cell Culture

    Localization of intracellular NTHi. BEAS-2B cells were infected with NTHi strain ATCC 19418 for 2 hours. After killing extracellular bacteria with gentamicin, epithelial cells and bacteria were stained with several fluorescent dyes and Hoechst (blue). a Representative fluorescent micrographs of intracellular NTHi (blue) and epithelial cells stained with early endosomal marker (EEA-1, red). White arrows show EEA-1-positive regions, and white arrowheads show NTHi. For a detail of merged image, see a top-right inset. b Representative fluorescence micrographs of intracellular NTHi (blue) and epithelial cells stained with late endosomal marker (LAMP-1, purple). White arrows show LAMP-1-positive regions, and white arrowheads show NTHi. For a detail of merged image, see a top-right inset. c Representative fluorescent micrographs of intracellular NTHi and epithelial cells stained with LIVE/DEAD® (green) and cells were stained with acidic lysosomal dye (LysoTracker® Red). White arrows show LysoTracker® Red-positive acidic organelles, and white arrowheads show NTHi. Fluorescent micrographs were taken at 2,000× magnification. White bars represent 5 μm. Another strain (HUSM 0481) was also tested and similar results were obtained

    Journal: BMC Microbiology

    Article Title: Nontypeable Haemophilus influenzae exploits the interaction between protein-E and vitronectin for the adherence and invasion to bronchial epithelial cells

    doi: 10.1186/s12866-015-0600-8

    Figure Lengend Snippet: Localization of intracellular NTHi. BEAS-2B cells were infected with NTHi strain ATCC 19418 for 2 hours. After killing extracellular bacteria with gentamicin, epithelial cells and bacteria were stained with several fluorescent dyes and Hoechst (blue). a Representative fluorescent micrographs of intracellular NTHi (blue) and epithelial cells stained with early endosomal marker (EEA-1, red). White arrows show EEA-1-positive regions, and white arrowheads show NTHi. For a detail of merged image, see a top-right inset. b Representative fluorescence micrographs of intracellular NTHi (blue) and epithelial cells stained with late endosomal marker (LAMP-1, purple). White arrows show LAMP-1-positive regions, and white arrowheads show NTHi. For a detail of merged image, see a top-right inset. c Representative fluorescent micrographs of intracellular NTHi and epithelial cells stained with LIVE/DEAD® (green) and cells were stained with acidic lysosomal dye (LysoTracker® Red). White arrows show LysoTracker® Red-positive acidic organelles, and white arrowheads show NTHi. Fluorescent micrographs were taken at 2,000× magnification. White bars represent 5 μm. Another strain (HUSM 0481) was also tested and similar results were obtained

    Article Snippet: The percentage of BEAS-2B cells invaded by each NTHi strain is shown in Fig. . Pretreatment with PE 84–108 significantly reduced the percentage of invaded cells by either strain of NTHi (Fig. , ATCC 19418: p < 0.001, HUSM 0481: p < 0.01).

    Techniques: Infection, Staining, Marker, Fluorescence

    NTHi binding of immobilized vitronectin in the absence or presence of heparin. Human plasma vitronectin was bound to glass-bottomed dishes. NTHi was incubated on the plate-bound vitronectin for 30 minutes, and then the dishes were washed three times. In some experiments, plate-bound vitronectin was pretreated with heparin before incubation with NTHi. NTHi was stained with LIVE/DEAD®. Live NTHi is stained green. BSA was used as a negative control. Representative fluorescent micrographs show that both NTHi strains (ATCC 19418 ( a ) and HUSM 0481 ( b )) attached to plate-bound vitronectin and that this attachment is blocked by heparin. White bars represent 10 μm. Summaries of the numbers of attached NTHi (per field at 1,000× magnification) are shown in ( c ) for ATCC 19418 and ( d ) for HUSM 0481. Error bars represent SEM in three independent experiments that gave similar results. ** p < 0.01

    Journal: BMC Microbiology

    Article Title: Nontypeable Haemophilus influenzae exploits the interaction between protein-E and vitronectin for the adherence and invasion to bronchial epithelial cells

    doi: 10.1186/s12866-015-0600-8

    Figure Lengend Snippet: NTHi binding of immobilized vitronectin in the absence or presence of heparin. Human plasma vitronectin was bound to glass-bottomed dishes. NTHi was incubated on the plate-bound vitronectin for 30 minutes, and then the dishes were washed three times. In some experiments, plate-bound vitronectin was pretreated with heparin before incubation with NTHi. NTHi was stained with LIVE/DEAD®. Live NTHi is stained green. BSA was used as a negative control. Representative fluorescent micrographs show that both NTHi strains (ATCC 19418 ( a ) and HUSM 0481 ( b )) attached to plate-bound vitronectin and that this attachment is blocked by heparin. White bars represent 10 μm. Summaries of the numbers of attached NTHi (per field at 1,000× magnification) are shown in ( c ) for ATCC 19418 and ( d ) for HUSM 0481. Error bars represent SEM in three independent experiments that gave similar results. ** p < 0.01

    Article Snippet: The percentage of BEAS-2B cells invaded by each NTHi strain is shown in Fig. . Pretreatment with PE 84–108 significantly reduced the percentage of invaded cells by either strain of NTHi (Fig. , ATCC 19418: p < 0.001, HUSM 0481: p < 0.01).

    Techniques: Binding Assay, Incubation, Staining, Negative Control

    Intracellular invasion of NTHi in the presence of heparin or RGD peptide. BEAS-2B cells were infected for 2 hours with one of the two NTHi strains (ATCC 19418 or HUSM 0481), E. coli (a negative control), or L. monocytogenes (a positive control). In some experiments, cells were pretreated with heparin or RGD-peptide. a After killing extracellular bacteria with gentamicin, epithelial cells and bacteria were stained with LIVE/DEAD®. The percentages of BEAS-2B cells invaded by each type of bacteria are shown. b After killing extracellular bacteria with gentamicin and lysing the BEAS-2B cells, the bacteria were cultured overnight. The number of colonies was counted and the percentages of CFU after gentamicin treatment of cells per input CFU were shown. Error bars represent SEM in three independent experiments that gave similar results. * p < 0.05 and ** p < 0.01

    Journal: BMC Microbiology

    Article Title: Nontypeable Haemophilus influenzae exploits the interaction between protein-E and vitronectin for the adherence and invasion to bronchial epithelial cells

    doi: 10.1186/s12866-015-0600-8

    Figure Lengend Snippet: Intracellular invasion of NTHi in the presence of heparin or RGD peptide. BEAS-2B cells were infected for 2 hours with one of the two NTHi strains (ATCC 19418 or HUSM 0481), E. coli (a negative control), or L. monocytogenes (a positive control). In some experiments, cells were pretreated with heparin or RGD-peptide. a After killing extracellular bacteria with gentamicin, epithelial cells and bacteria were stained with LIVE/DEAD®. The percentages of BEAS-2B cells invaded by each type of bacteria are shown. b After killing extracellular bacteria with gentamicin and lysing the BEAS-2B cells, the bacteria were cultured overnight. The number of colonies was counted and the percentages of CFU after gentamicin treatment of cells per input CFU were shown. Error bars represent SEM in three independent experiments that gave similar results. * p < 0.05 and ** p < 0.01

    Article Snippet: The percentage of BEAS-2B cells invaded by each NTHi strain is shown in Fig. . Pretreatment with PE 84–108 significantly reduced the percentage of invaded cells by either strain of NTHi (Fig. , ATCC 19418: p < 0.001, HUSM 0481: p < 0.01).

    Techniques: Infection, Negative Control, Positive Control, Staining, Cell Culture

    Binding of NTHi to immobilized vitronectin in the presence of protein-E peptide. Human plasma vitronectin was bound to glass-bottomed dishes. NTHi (HUSM 0481) was incubated on the plate-bound vitronectin for 30 minutes, and then the dishes were washed three times. In some experiments, plate-bound vitronectin was pretreated with protein-E peptide (PE 84–108 ) before incubation with NTHi. BSA was used as a negative control. NTHi was stained with LIVE/DEAD®, and viable NTHi is stained green. a Representative fluorescent micrographs of NTHi incubated on plate-bound vitronectin that was untreated or pretreated with PE 84–108 . White bars represent 10 μm. b The number of attached NTHi per field at 1,000 × magnification that was pretreated with increasing concentrations of PE 84–108 . Error bars represent SEM in three independent experiments that gave similar results. ** p < 0.01

    Journal: BMC Microbiology

    Article Title: Nontypeable Haemophilus influenzae exploits the interaction between protein-E and vitronectin for the adherence and invasion to bronchial epithelial cells

    doi: 10.1186/s12866-015-0600-8

    Figure Lengend Snippet: Binding of NTHi to immobilized vitronectin in the presence of protein-E peptide. Human plasma vitronectin was bound to glass-bottomed dishes. NTHi (HUSM 0481) was incubated on the plate-bound vitronectin for 30 minutes, and then the dishes were washed three times. In some experiments, plate-bound vitronectin was pretreated with protein-E peptide (PE 84–108 ) before incubation with NTHi. BSA was used as a negative control. NTHi was stained with LIVE/DEAD®, and viable NTHi is stained green. a Representative fluorescent micrographs of NTHi incubated on plate-bound vitronectin that was untreated or pretreated with PE 84–108 . White bars represent 10 μm. b The number of attached NTHi per field at 1,000 × magnification that was pretreated with increasing concentrations of PE 84–108 . Error bars represent SEM in three independent experiments that gave similar results. ** p < 0.01

    Article Snippet: The percentage of BEAS-2B cells invaded by each NTHi strain is shown in Fig. . Pretreatment with PE 84–108 significantly reduced the percentage of invaded cells by either strain of NTHi (Fig. , ATCC 19418: p < 0.001, HUSM 0481: p < 0.01).

    Techniques: Binding Assay, Incubation, Negative Control, Staining

    Intracellular invasion of NTHi in the absence or presence of protein-E. BEAS-2B cells were infected for 2 hours with one of the two NTHi strains (ATCC 19418 or HUSM 0481) with or without pretreatment with PE 84–108 peptide. a After killing extracellular bacteria with gentamicin, epithelial cells and bacteria were stained with LIVE/DEAD®. The percentages of BEAS-2B cells invaded by each NTHi strain are shown. b After killing extracellular bacteria with gentamicin, cells were lysed and the bacteria were cultured overnight. The number of colonies was counted and the percentages of CFU after gentamicin treatment of cells per input CFU were shown. Error bars represent SEM in three independent experiments that gave similar results. * p < 0.05 and ** p < 0.01

    Journal: BMC Microbiology

    Article Title: Nontypeable Haemophilus influenzae exploits the interaction between protein-E and vitronectin for the adherence and invasion to bronchial epithelial cells

    doi: 10.1186/s12866-015-0600-8

    Figure Lengend Snippet: Intracellular invasion of NTHi in the absence or presence of protein-E. BEAS-2B cells were infected for 2 hours with one of the two NTHi strains (ATCC 19418 or HUSM 0481) with or without pretreatment with PE 84–108 peptide. a After killing extracellular bacteria with gentamicin, epithelial cells and bacteria were stained with LIVE/DEAD®. The percentages of BEAS-2B cells invaded by each NTHi strain are shown. b After killing extracellular bacteria with gentamicin, cells were lysed and the bacteria were cultured overnight. The number of colonies was counted and the percentages of CFU after gentamicin treatment of cells per input CFU were shown. Error bars represent SEM in three independent experiments that gave similar results. * p < 0.05 and ** p < 0.01

    Article Snippet: The percentage of BEAS-2B cells invaded by each NTHi strain is shown in Fig. . Pretreatment with PE 84–108 significantly reduced the percentage of invaded cells by either strain of NTHi (Fig. , ATCC 19418: p < 0.001, HUSM 0481: p < 0.01).

    Techniques: Infection, Staining, Cell Culture