Structured Review

Thermo Fisher nsi
Nsi, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/nsi/product/Thermo Fisher
Average 90 stars, based on 2 article reviews
Price from $9.99 to $1999.99
nsi - by Bioz Stars, 2020-01
90/100 stars

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Clone Assay:

Article Title: Evaluation of antibody fragment properties for near-infrared fluorescence imaging of HER3-positive cancer xenografts
Article Snippet: .. Amplicons were cloned into pFUSEss-CHIg-hG1 vector digested with EcoRI and Nsi (Thermo Fisher Scientific) using Gibson Assembly™ master mix (New England Biolabs, Inc., Ipswich, MA). .. Anti-HER3 IgG1 expression vector was constructed by PCR amplifying VH and VL sequences and cloning them into EcoRI/NheI and EcoRI/BsiWI restriction sites in pFUSEss-CHIg-hG1 and pFUSE2ss-CLIg-hk (InvivoGen, San Diego, CA), respectively using Gibson Assembly™ protocol.

Article Title: Thioredoxin h5 Is Required for Victorin Sensitivity Mediated by a CC-NBS-LRR Gene in Arabidopsis [W]
Article Snippet: Each cDNA was amplified by PCR and cloned into pENTR/D-TOPO (Invitrogen) using the following primers: ATTRX5 ENTR forward, 5′-CACCATGGCCGGTGAAGGAGA-3′; ATTRX5 ENTR reverse, 5′-TCAAGCAGAAGCTACAAGACCA-3′; ATTRX3 ENTR forward, 5′-CACCATGGCCGCAGAAGGAG-3′; ATTRX3 ENTR reverse, 5′-TCAAGCAGCAGCAACAACTGT-3′. .. Each pENTR clone was digested with Nsi I, and the fragment containing the cDNA was gel-purified and recombined into the binary vector pEarleyGate 100 ( ) using Gateway LR Clonase II enzyme mix (Invitrogen) according to the manufacturer's instructions.

Flow Cytometry:

Article Title: Dynamic remodeling of membrane composition drives cell cycle through primary cilia excision
Article Snippet: Peptides were loaded into a micro capillary column (NTCC-360; Nikkyo Technos, Japan) of 75 µm inner diameter, and separated with a linear gradient of acetonitrile from 0 to 35% in 0.1% formic acid at a flow rate of 325 nL/min. .. Separated peptides were ionized with Nanospray Flex Ion Source, NSI (ThermoFisher) at spray voltage of 2.0 kV.

Amplification:

Article Title: Evaluation of antibody fragment properties for near-infrared fluorescence imaging of HER3-positive cancer xenografts
Article Snippet: Conversion of Anti-HER3 scFv to scFv-Fc, scFv-CH 3, and IgG The CH 3 domain with hinge region (CH 3-hinge) or CH 3-CH 2-hinge (Fc) from human IgG1 were PCR amplified from pFUSEss-CHIg-hG1 mammalian expression vector (InvivoGen, San Diego, CA) using primers that contain overlapping sequences with anti-HER3 scFv and then assembled into scFv-CH 3 or scFv-Fc using gene SOEing . .. Amplicons were cloned into pFUSEss-CHIg-hG1 vector digested with EcoRI and Nsi (Thermo Fisher Scientific) using Gibson Assembly™ master mix (New England Biolabs, Inc., Ipswich, MA).

Article Title: Thioredoxin h5 Is Required for Victorin Sensitivity Mediated by a CC-NBS-LRR Gene in Arabidopsis [W]
Article Snippet: Each cDNA was amplified by PCR and cloned into pENTR/D-TOPO (Invitrogen) using the following primers: ATTRX5 ENTR forward, 5′-CACCATGGCCGGTGAAGGAGA-3′; ATTRX5 ENTR reverse, 5′-TCAAGCAGAAGCTACAAGACCA-3′; ATTRX3 ENTR forward, 5′-CACCATGGCCGCAGAAGGAG-3′; ATTRX3 ENTR reverse, 5′-TCAAGCAGCAGCAACAACTGT-3′. .. Each pENTR clone was digested with Nsi I, and the fragment containing the cDNA was gel-purified and recombined into the binary vector pEarleyGate 100 ( ) using Gateway LR Clonase II enzyme mix (Invitrogen) according to the manufacturer's instructions.

Expressing:

Article Title: Evaluation of antibody fragment properties for near-infrared fluorescence imaging of HER3-positive cancer xenografts
Article Snippet: Conversion of Anti-HER3 scFv to scFv-Fc, scFv-CH 3, and IgG The CH 3 domain with hinge region (CH 3-hinge) or CH 3-CH 2-hinge (Fc) from human IgG1 were PCR amplified from pFUSEss-CHIg-hG1 mammalian expression vector (InvivoGen, San Diego, CA) using primers that contain overlapping sequences with anti-HER3 scFv and then assembled into scFv-CH 3 or scFv-Fc using gene SOEing . .. Amplicons were cloned into pFUSEss-CHIg-hG1 vector digested with EcoRI and Nsi (Thermo Fisher Scientific) using Gibson Assembly™ master mix (New England Biolabs, Inc., Ipswich, MA).

Mutagenesis:

Article Title: Thioredoxin h5 Is Required for Victorin Sensitivity Mediated by a CC-NBS-LRR Gene in Arabidopsis [W]
Article Snippet: Each pENTR clone was digested with Nsi I, and the fragment containing the cDNA was gel-purified and recombined into the binary vector pEarleyGate 100 ( ) using Gateway LR Clonase II enzyme mix (Invitrogen) according to the manufacturer's instructions. .. Plants containing the attrx5-1 mutation were transformed as described above.

Isolation:

Article Title: Dynamic remodeling of membrane composition drives cell cycle through primary cilia excision
Article Snippet: Separated peptides were ionized with Nanospray Flex Ion Source, NSI (ThermoFisher) at spray voltage of 2.0 kV. .. Full mass spectra were acquired with a resolution of 70,000, a maximum injection time of 60 msec, and a scan range between m/z 350 to 1800, at positive ion mode. dd-MS2 was acquired with a resolution of 17,500, a maximum injection time of 60 msec, and an isolation window of 2.0 m/z .

Construct:

Article Title: Evaluation of antibody fragment properties for near-infrared fluorescence imaging of HER3-positive cancer xenografts
Article Snippet: Amplicons were cloned into pFUSEss-CHIg-hG1 vector digested with EcoRI and Nsi (Thermo Fisher Scientific) using Gibson Assembly™ master mix (New England Biolabs, Inc., Ipswich, MA). .. Anti-HER3 IgG1 expression vector was constructed by PCR amplifying VH and VL sequences and cloning them into EcoRI/NheI and EcoRI/BsiWI restriction sites in pFUSEss-CHIg-hG1 and pFUSE2ss-CLIg-hk (InvivoGen, San Diego, CA), respectively using Gibson Assembly™ protocol.

Article Title: Thioredoxin h5 Is Required for Victorin Sensitivity Mediated by a CC-NBS-LRR Gene in Arabidopsis [W]
Article Snippet: Paragraph title: Creation of pEarleyGate Overexpression Constructs ... Each pENTR clone was digested with Nsi I, and the fragment containing the cDNA was gel-purified and recombined into the binary vector pEarleyGate 100 ( ) using Gateway LR Clonase II enzyme mix (Invitrogen) according to the manufacturer's instructions.

Liquid Chromatography:

Article Title: Dynamic remodeling of membrane composition drives cell cycle through primary cilia excision
Article Snippet: The peptides were analyzed with a Q Exactive-Orbitrap mass spectrometer (ThermoFisher) connected to EASY-nLC liquid chromatography (ThermoFisher). .. Separated peptides were ionized with Nanospray Flex Ion Source, NSI (ThermoFisher) at spray voltage of 2.0 kV.

Transgenic Assay:

Article Title: Thioredoxin h5 Is Required for Victorin Sensitivity Mediated by a CC-NBS-LRR Gene in Arabidopsis [W]
Article Snippet: Each pENTR clone was digested with Nsi I, and the fragment containing the cDNA was gel-purified and recombined into the binary vector pEarleyGate 100 ( ) using Gateway LR Clonase II enzyme mix (Invitrogen) according to the manufacturer's instructions. .. Putative transgenic seed were planted in soil wet with 0.02% glufosinate-ammonium.

Mass Spectrometry:

Article Title: Dynamic remodeling of membrane composition drives cell cycle through primary cilia excision
Article Snippet: The peptides were analyzed with a Q Exactive-Orbitrap mass spectrometer (ThermoFisher) connected to EASY-nLC liquid chromatography (ThermoFisher). .. Separated peptides were ionized with Nanospray Flex Ion Source, NSI (ThermoFisher) at spray voltage of 2.0 kV.

Polymerase Chain Reaction:

Article Title: Evaluation of antibody fragment properties for near-infrared fluorescence imaging of HER3-positive cancer xenografts
Article Snippet: Conversion of Anti-HER3 scFv to scFv-Fc, scFv-CH 3, and IgG The CH 3 domain with hinge region (CH 3-hinge) or CH 3-CH 2-hinge (Fc) from human IgG1 were PCR amplified from pFUSEss-CHIg-hG1 mammalian expression vector (InvivoGen, San Diego, CA) using primers that contain overlapping sequences with anti-HER3 scFv and then assembled into scFv-CH 3 or scFv-Fc using gene SOEing . .. Amplicons were cloned into pFUSEss-CHIg-hG1 vector digested with EcoRI and Nsi (Thermo Fisher Scientific) using Gibson Assembly™ master mix (New England Biolabs, Inc., Ipswich, MA).

Article Title: Thioredoxin h5 Is Required for Victorin Sensitivity Mediated by a CC-NBS-LRR Gene in Arabidopsis [W]
Article Snippet: Each cDNA was amplified by PCR and cloned into pENTR/D-TOPO (Invitrogen) using the following primers: ATTRX5 ENTR forward, 5′-CACCATGGCCGGTGAAGGAGA-3′; ATTRX5 ENTR reverse, 5′-TCAAGCAGAAGCTACAAGACCA-3′; ATTRX3 ENTR forward, 5′-CACCATGGCCGCAGAAGGAG-3′; ATTRX3 ENTR reverse, 5′-TCAAGCAGCAGCAACAACTGT-3′. .. Each pENTR clone was digested with Nsi I, and the fragment containing the cDNA was gel-purified and recombined into the binary vector pEarleyGate 100 ( ) using Gateway LR Clonase II enzyme mix (Invitrogen) according to the manufacturer's instructions.

Transformation Assay:

Article Title: Thioredoxin h5 Is Required for Victorin Sensitivity Mediated by a CC-NBS-LRR Gene in Arabidopsis [W]
Article Snippet: Each pENTR clone was digested with Nsi I, and the fragment containing the cDNA was gel-purified and recombined into the binary vector pEarleyGate 100 ( ) using Gateway LR Clonase II enzyme mix (Invitrogen) according to the manufacturer's instructions. .. The recombination reactions were transformed into Escherichia coli strain DH5α, and the resulting pEarleyGate clones were introduced into Agrobacterium strain GV3101.

Over Expression:

Article Title: Thioredoxin h5 Is Required for Victorin Sensitivity Mediated by a CC-NBS-LRR Gene in Arabidopsis [W]
Article Snippet: Paragraph title: Creation of pEarleyGate Overexpression Constructs ... Each pENTR clone was digested with Nsi I, and the fragment containing the cDNA was gel-purified and recombined into the binary vector pEarleyGate 100 ( ) using Gateway LR Clonase II enzyme mix (Invitrogen) according to the manufacturer's instructions.

Injection:

Article Title: Dynamic remodeling of membrane composition drives cell cycle through primary cilia excision
Article Snippet: Separated peptides were ionized with Nanospray Flex Ion Source, NSI (ThermoFisher) at spray voltage of 2.0 kV. .. Full mass spectra were acquired with a resolution of 70,000, a maximum injection time of 60 msec, and a scan range between m/z 350 to 1800, at positive ion mode. dd-MS2 was acquired with a resolution of 17,500, a maximum injection time of 60 msec, and an isolation window of 2.0 m/z .

Plasmid Preparation:

Article Title: Evaluation of antibody fragment properties for near-infrared fluorescence imaging of HER3-positive cancer xenografts
Article Snippet: .. Amplicons were cloned into pFUSEss-CHIg-hG1 vector digested with EcoRI and Nsi (Thermo Fisher Scientific) using Gibson Assembly™ master mix (New England Biolabs, Inc., Ipswich, MA). .. Anti-HER3 IgG1 expression vector was constructed by PCR amplifying VH and VL sequences and cloning them into EcoRI/NheI and EcoRI/BsiWI restriction sites in pFUSEss-CHIg-hG1 and pFUSE2ss-CLIg-hk (InvivoGen, San Diego, CA), respectively using Gibson Assembly™ protocol.

Article Title: Thioredoxin h5 Is Required for Victorin Sensitivity Mediated by a CC-NBS-LRR Gene in Arabidopsis [W]
Article Snippet: .. Each pENTR clone was digested with Nsi I, and the fragment containing the cDNA was gel-purified and recombined into the binary vector pEarleyGate 100 ( ) using Gateway LR Clonase II enzyme mix (Invitrogen) according to the manufacturer's instructions. .. The recombination reactions were transformed into Escherichia coli strain DH5α, and the resulting pEarleyGate clones were introduced into Agrobacterium strain GV3101.

Software:

Article Title: Dynamic remodeling of membrane composition drives cell cycle through primary cilia excision
Article Snippet: Separated peptides were ionized with Nanospray Flex Ion Source, NSI (ThermoFisher) at spray voltage of 2.0 kV. .. All processes were operated with software XcaliburTM (ThermoFisher).

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    Reduce energy emissions while increasing worker safety reducing capital costs and ensuring global ECO requirements The Thermo Scientific DensityPRO NAI non contacting gauge uses a smaller source that emits up
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    Thermo Fisher lc nsi hrms ms
    Typical <t>LC–NSI–HRMS/MS</t> chromatograms obtained upon analysis of 5,6-dihydro-M 1 dG in human leukocyte DNA from a (A) smoker and (B) nonsmoker.
    Lc Nsi Hrms Ms, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 81/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lc nsi hrms ms/product/Thermo Fisher
    Average 81 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    lc nsi hrms ms - by Bioz Stars, 2020-01
    81/100 stars
      Buy from Supplier

    79
    Thermo Fisher lc nsi ms ms
    Typical <t>LC–NSI–HRMS/MS</t> chromatograms obtained upon analysis of 5,6-dihydro-M 1 dG in human leukocyte DNA from a (A) smoker and (B) nonsmoker.
    Lc Nsi Ms Ms, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 79/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lc nsi ms ms/product/Thermo Fisher
    Average 79 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    lc nsi ms ms - by Bioz Stars, 2020-01
    79/100 stars
      Buy from Supplier

    Image Search Results


    Typical LC–NSI–HRMS/MS chromatograms obtained upon analysis of 5,6-dihydro-M 1 dG in human leukocyte DNA from a (A) smoker and (B) nonsmoker.

    Journal: Chemical Research in Toxicology

    Article Title: Analysis of a Malondialdehyde–Deoxyguanosine Adduct in Human Leukocyte DNA by Liquid Chromatography Nanoelectrospray–High-Resolution Tandem Mass Spectrometry

    doi: 10.1021/tx5002699

    Figure Lengend Snippet: Typical LC–NSI–HRMS/MS chromatograms obtained upon analysis of 5,6-dihydro-M 1 dG in human leukocyte DNA from a (A) smoker and (B) nonsmoker.

    Article Snippet: LC–Nanoelectrospray Ionization (NSI)–High-Resolution (HR) MS/MS The LC–NSI–HRMS/MS was performed on an LTQ Orbitrap Velos instrument (Thermo Scientific, Waltham, MA) interfaced with a Nano2D–LC HPLC (Eksigent, Dublin, CA) system using nanoelectrospray ionization.

    Techniques: Mass Spectrometry

    Chromatograms obtained upon analysis of M 1 dG in the same human leukocyte DNA sample by using (A) LC–ESI–MS/MS and (B) LC–NSI–HRMS/MS.

    Journal: Chemical Research in Toxicology

    Article Title: Analysis of a Malondialdehyde–Deoxyguanosine Adduct in Human Leukocyte DNA by Liquid Chromatography Nanoelectrospray–High-Resolution Tandem Mass Spectrometry

    doi: 10.1021/tx5002699

    Figure Lengend Snippet: Chromatograms obtained upon analysis of M 1 dG in the same human leukocyte DNA sample by using (A) LC–ESI–MS/MS and (B) LC–NSI–HRMS/MS.

    Article Snippet: LC–Nanoelectrospray Ionization (NSI)–High-Resolution (HR) MS/MS The LC–NSI–HRMS/MS was performed on an LTQ Orbitrap Velos instrument (Thermo Scientific, Waltham, MA) interfaced with a Nano2D–LC HPLC (Eksigent, Dublin, CA) system using nanoelectrospray ionization.

    Techniques: Mass Spectrometry

    Chromatograms obtained upon LC-NSI-HRMS/MS analysis of human leukocyte DNA (129 μg, 12.9 μg on column) containing 59.4 fmol N7-ethyl-Gua /μmol Gua. The relatively higher amount of analyte in this sample allowed for the confirmation

    Journal: Chemical reviews

    Article Title: Mass Spectrometry of Structurally Modified DNA

    doi: 10.1021/cr300391r

    Figure Lengend Snippet: Chromatograms obtained upon LC-NSI-HRMS/MS analysis of human leukocyte DNA (129 μg, 12.9 μg on column) containing 59.4 fmol N7-ethyl-Gua /μmol Gua. The relatively higher amount of analyte in this sample allowed for the confirmation

    Article Snippet: The LC-NSI-HRMS/MS method used LTQ Orbitrap Velos instrument (Thermo Scientific, Waltham, MA) and nanospray source (Thermo Scientific) and a Nano2D-LC HPLC (Eksigent, Dublin, CA).

    Techniques: Mass Spectrometry