nsi sources  (Thermo Fisher)


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    Structured Review

    Thermo Fisher nsi sources
    Nsi Sources, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nsi sources/product/Thermo Fisher
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    nsi sources - by Bioz Stars, 2020-04
    91/100 stars

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    Related Articles

    Flow Cytometry:

    Article Title: Outer Membrane Vesicles as a Candidate Vaccine against Edwardsiellosis
    Article Snippet: LC-ESI-MS/MS and data analysis LC-ESI-MS/MS (liquid chromatography electrospray ionization tandem mass spectrometry) analysis was carried out using a Thermo Finnigan Proteome X workstation with an LTQ linear ion trap and a MS apparatus equipped with NSI sources (Thermo Electron, San Jose, CA). .. The mobile phase solutions were H2 O and ACN, both containing 0.1% (v/v) formic acid, and delivered at a constant flow rate of 0.2 µl/min.

    Mass Spectrometry:

    Article Title: NecroX-5 protects mitochondrial oxidative phosphorylation capacity and preserves PGC1α expression levels during hypoxia/reoxygenation injury
    Article Snippet: .. LC-MS/MS analysis was performed using a ThermoFinnigan ProteomeX workstation LTQ linear ion trap MS (Thermo Electron, San Jose, CA) equipped with NSI sources (Thermo Electron). .. Protein annotation and functional network construction Identified proteins were further categorized, annotated, and functional networks were constructed to understand the molecular functions and biological processes of each protein [ ].

    Article Title: Study of Treadmill Exercise Effect on Rats with Osteoarthritis Using Proteomic Analysis
    Article Snippet: .. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis was carried out using an LTQ mass spectrometer (Thermo Electron, San Jose, CA, USA) equipped with NSI sources (Thermo Electron, San Jose, CA), as previously reported ) . .. Briefly, 12 ul of peptide sample from in-gel digestion was injected and loaded onto a peptide trap cartridge (Agilent, Palo Alto, CA, USA).

    Article Title: NecroX-5 exerts anti-inflammatory and anti-fibrotic effects via modulation of the TNFα/Dcn/TGFβ1/Smad2 pathway in hypoxia/reoxygenation-treated rat hearts
    Article Snippet: .. LC-MS/MS analysis was performed using a ThermoFinnigan ProteomeX workstation LTQ linear ion trap MS equipped with NSI sources (Thermo Electron, San Jose, CA, USA). .. Protein annotation and functional network construction In order to elucidate the molecular functions and biological processes of the identified proteins, the proteins were further categorized and annotated, and functional networks were constructed.

    Article Title: Outer Membrane Vesicles as a Candidate Vaccine against Edwardsiellosis
    Article Snippet: .. LC-ESI-MS/MS and data analysis LC-ESI-MS/MS (liquid chromatography electrospray ionization tandem mass spectrometry) analysis was carried out using a Thermo Finnigan Proteome X workstation with an LTQ linear ion trap and a MS apparatus equipped with NSI sources (Thermo Electron, San Jose, CA). .. Twelve microliter amounts of peptide mixtures were injected and loaded onto peptide trap cartridges (Agilent, Palo Alto, CA).

    Chromatography:

    Article Title: Study of Treadmill Exercise Effect on Rats with Osteoarthritis Using Proteomic Analysis
    Article Snippet: .. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis was carried out using an LTQ mass spectrometer (Thermo Electron, San Jose, CA, USA) equipped with NSI sources (Thermo Electron, San Jose, CA), as previously reported ) . .. Briefly, 12 ul of peptide sample from in-gel digestion was injected and loaded onto a peptide trap cartridge (Agilent, Palo Alto, CA, USA).

    SDS Page:

    Article Title: NecroX-5 protects mitochondrial oxidative phosphorylation capacity and preserves PGC1α expression levels during hypoxia/reoxygenation injury
    Article Snippet: Briefly, dissolved heart proteins were separated on a 12% polyacrylamide gel by SDS-PAGE. .. LC-MS/MS analysis was performed using a ThermoFinnigan ProteomeX workstation LTQ linear ion trap MS (Thermo Electron, San Jose, CA) equipped with NSI sources (Thermo Electron).

    Article Title: NecroX-5 exerts anti-inflammatory and anti-fibrotic effects via modulation of the TNFα/Dcn/TGFβ1/Smad2 pathway in hypoxia/reoxygenation-treated rat hearts
    Article Snippet: Heart proteins were separated via SDS-PAGE on a 12% polyacrylamide gel. .. LC-MS/MS analysis was performed using a ThermoFinnigan ProteomeX workstation LTQ linear ion trap MS equipped with NSI sources (Thermo Electron, San Jose, CA, USA).

    Protein Extraction:

    Article Title: NecroX-5 protects mitochondrial oxidative phosphorylation capacity and preserves PGC1α expression levels during hypoxia/reoxygenation injury
    Article Snippet: Paragraph title: Protein extraction for LC-MS and peptide count protein quantification ... LC-MS/MS analysis was performed using a ThermoFinnigan ProteomeX workstation LTQ linear ion trap MS (Thermo Electron, San Jose, CA) equipped with NSI sources (Thermo Electron).

    Article Title: NecroX-5 exerts anti-inflammatory and anti-fibrotic effects via modulation of the TNFα/Dcn/TGFβ1/Smad2 pathway in hypoxia/reoxygenation-treated rat hearts
    Article Snippet: Paragraph title: Protein extraction for LC-MS and peptide count protein quantification ... LC-MS/MS analysis was performed using a ThermoFinnigan ProteomeX workstation LTQ linear ion trap MS equipped with NSI sources (Thermo Electron, San Jose, CA, USA).

    Liquid Chromatography with Mass Spectroscopy:

    Article Title: NecroX-5 protects mitochondrial oxidative phosphorylation capacity and preserves PGC1α expression levels during hypoxia/reoxygenation injury
    Article Snippet: Paragraph title: Protein extraction for LC-MS and peptide count protein quantification ... LC-MS/MS analysis was performed using a ThermoFinnigan ProteomeX workstation LTQ linear ion trap MS (Thermo Electron, San Jose, CA) equipped with NSI sources (Thermo Electron).

    Article Title: NecroX-5 exerts anti-inflammatory and anti-fibrotic effects via modulation of the TNFα/Dcn/TGFβ1/Smad2 pathway in hypoxia/reoxygenation-treated rat hearts
    Article Snippet: Paragraph title: Protein extraction for LC-MS and peptide count protein quantification ... LC-MS/MS analysis was performed using a ThermoFinnigan ProteomeX workstation LTQ linear ion trap MS equipped with NSI sources (Thermo Electron, San Jose, CA, USA).

    Modification:

    Article Title: Study of Treadmill Exercise Effect on Rats with Osteoarthritis Using Proteomic Analysis
    Article Snippet: The running protocol was performed according to a modified version of Ueda’s method , ) . .. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis was carried out using an LTQ mass spectrometer (Thermo Electron, San Jose, CA, USA) equipped with NSI sources (Thermo Electron, San Jose, CA), as previously reported ) .

    Injection:

    Article Title: Study of Treadmill Exercise Effect on Rats with Osteoarthritis Using Proteomic Analysis
    Article Snippet: Rats were randomly divided into five groups: (1) no MIA injection or exercise group (control group, n = 10), (2) MIA injection without treadmill running exercise group (MIA injection group, n = 10), (3) MIA injection with low-intensity exercise group (LE, n = 10), (4) MIA injection with middle-intensity exercise group (ME, n = 10), and (5) MIA injection and with high-intensity exercise group (HE, n = 10). .. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis was carried out using an LTQ mass spectrometer (Thermo Electron, San Jose, CA, USA) equipped with NSI sources (Thermo Electron, San Jose, CA), as previously reported ) .

    Article Title: Outer Membrane Vesicles as a Candidate Vaccine against Edwardsiellosis
    Article Snippet: LC-ESI-MS/MS and data analysis LC-ESI-MS/MS (liquid chromatography electrospray ionization tandem mass spectrometry) analysis was carried out using a Thermo Finnigan Proteome X workstation with an LTQ linear ion trap and a MS apparatus equipped with NSI sources (Thermo Electron, San Jose, CA). .. Twelve microliter amounts of peptide mixtures were injected and loaded onto peptide trap cartridges (Agilent, Palo Alto, CA).

    Liquid Chromatography:

    Article Title: Outer Membrane Vesicles as a Candidate Vaccine against Edwardsiellosis
    Article Snippet: .. LC-ESI-MS/MS and data analysis LC-ESI-MS/MS (liquid chromatography electrospray ionization tandem mass spectrometry) analysis was carried out using a Thermo Finnigan Proteome X workstation with an LTQ linear ion trap and a MS apparatus equipped with NSI sources (Thermo Electron, San Jose, CA). .. Twelve microliter amounts of peptide mixtures were injected and loaded onto peptide trap cartridges (Agilent, Palo Alto, CA).

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  • 91
    Thermo Fisher nsi ms
    <t>NSI-MS</t> spectra of permethylated N -glycans from platelets of saline (SAL) (A) and 5-HT-infused (B) mice. An equal number of platelets from WT mice infused for 24 hr with SAL or 5-HT were collected and plasma membrane (PM) was isolated 34 . The N -linked glycans on membrane vesicles were released enzymatically by PNGase F. Released N -glycans were permethylated and profiled by an LTQ Orbitrap Discoverer mass spectrometer equipped with a <t>nanospray</t> ion source. Glycans are detected as singly [M + Na] + , doubly [M + Na] 2+ and triply [M + Na] 3+ charged species. Structural assignments are based on MS/MS fragmentation and known biosynthetic limitation 38 39 . (C) The relative abundance of Neu5Ac on the platelet PM of SAL-infused mice was 2.9-fold higher than Neu5Gc containing glycans, whereas the ratio for platelet PM of 5-HT-infused mice was 3-fold higher in Neu5Gc-containing glycans than Neu5Ac. The total Neu5Ac and Neu5Gc-containing glycans was not different between platelet PM of SAL and 5-HT mice.
    Nsi Ms, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nsi ms/product/Thermo Fisher
    Average 91 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    nsi ms - by Bioz Stars, 2020-04
    91/100 stars
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    NSI-MS spectra of permethylated N -glycans from platelets of saline (SAL) (A) and 5-HT-infused (B) mice. An equal number of platelets from WT mice infused for 24 hr with SAL or 5-HT were collected and plasma membrane (PM) was isolated 34 . The N -linked glycans on membrane vesicles were released enzymatically by PNGase F. Released N -glycans were permethylated and profiled by an LTQ Orbitrap Discoverer mass spectrometer equipped with a nanospray ion source. Glycans are detected as singly [M + Na] + , doubly [M + Na] 2+ and triply [M + Na] 3+ charged species. Structural assignments are based on MS/MS fragmentation and known biosynthetic limitation 38 39 . (C) The relative abundance of Neu5Ac on the platelet PM of SAL-infused mice was 2.9-fold higher than Neu5Gc containing glycans, whereas the ratio for platelet PM of 5-HT-infused mice was 3-fold higher in Neu5Gc-containing glycans than Neu5Ac. The total Neu5Ac and Neu5Gc-containing glycans was not different between platelet PM of SAL and 5-HT mice.

    Journal: Scientific Reports

    Article Title: A serotonin-induced N-glycan switch regulates platelet aggregation

    doi: 10.1038/srep02795

    Figure Lengend Snippet: NSI-MS spectra of permethylated N -glycans from platelets of saline (SAL) (A) and 5-HT-infused (B) mice. An equal number of platelets from WT mice infused for 24 hr with SAL or 5-HT were collected and plasma membrane (PM) was isolated 34 . The N -linked glycans on membrane vesicles were released enzymatically by PNGase F. Released N -glycans were permethylated and profiled by an LTQ Orbitrap Discoverer mass spectrometer equipped with a nanospray ion source. Glycans are detected as singly [M + Na] + , doubly [M + Na] 2+ and triply [M + Na] 3+ charged species. Structural assignments are based on MS/MS fragmentation and known biosynthetic limitation 38 39 . (C) The relative abundance of Neu5Ac on the platelet PM of SAL-infused mice was 2.9-fold higher than Neu5Gc containing glycans, whereas the ratio for platelet PM of 5-HT-infused mice was 3-fold higher in Neu5Gc-containing glycans than Neu5Ac. The total Neu5Ac and Neu5Gc-containing glycans was not different between platelet PM of SAL and 5-HT mice.

    Article Snippet: Nanospray ionization-linear ion trap mass spectrometry Mass analysis by NSI-MS was performed on a LTQ Orbitrap mass spectrometer (Thermo Scientific) equipped with a nanospray ion source.

    Techniques: Mass Spectrometry, Mouse Assay, Isolation