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Thermo Fisher nsi ms
<t>NSI-MS</t> spectra of permethylated N -glycans from platelets of saline (SAL) (A) and 5-HT-infused (B) mice. An equal number of platelets from WT mice infused for 24 hr with SAL or 5-HT were collected and plasma membrane (PM) was isolated 34 . The N -linked glycans on membrane vesicles were released enzymatically by PNGase F. Released N -glycans were permethylated and profiled by an LTQ Orbitrap Discoverer mass spectrometer equipped with a <t>nanospray</t> ion source. Glycans are detected as singly [M + Na] + , doubly [M + Na] 2+ and triply [M + Na] 3+ charged species. Structural assignments are based on MS/MS fragmentation and known biosynthetic limitation 38 39 . (C) The relative abundance of Neu5Ac on the platelet PM of SAL-infused mice was 2.9-fold higher than Neu5Gc containing glycans, whereas the ratio for platelet PM of 5-HT-infused mice was 3-fold higher in Neu5Gc-containing glycans than Neu5Ac. The total Neu5Ac and Neu5Gc-containing glycans was not different between platelet PM of SAL and 5-HT mice.
Nsi Ms, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Article Title: A serotonin-induced N-glycan switch regulates platelet aggregation

Journal: Scientific Reports

doi: 10.1038/srep02795

NSI-MS spectra of permethylated N -glycans from platelets of saline (SAL) (A) and 5-HT-infused (B) mice. An equal number of platelets from WT mice infused for 24 hr with SAL or 5-HT were collected and plasma membrane (PM) was isolated 34 . The N -linked glycans on membrane vesicles were released enzymatically by PNGase F. Released N -glycans were permethylated and profiled by an LTQ Orbitrap Discoverer mass spectrometer equipped with a nanospray ion source. Glycans are detected as singly [M + Na] + , doubly [M + Na] 2+ and triply [M + Na] 3+ charged species. Structural assignments are based on MS/MS fragmentation and known biosynthetic limitation 38 39 . (C) The relative abundance of Neu5Ac on the platelet PM of SAL-infused mice was 2.9-fold higher than Neu5Gc containing glycans, whereas the ratio for platelet PM of 5-HT-infused mice was 3-fold higher in Neu5Gc-containing glycans than Neu5Ac. The total Neu5Ac and Neu5Gc-containing glycans was not different between platelet PM of SAL and 5-HT mice.
Figure Legend Snippet: NSI-MS spectra of permethylated N -glycans from platelets of saline (SAL) (A) and 5-HT-infused (B) mice. An equal number of platelets from WT mice infused for 24 hr with SAL or 5-HT were collected and plasma membrane (PM) was isolated 34 . The N -linked glycans on membrane vesicles were released enzymatically by PNGase F. Released N -glycans were permethylated and profiled by an LTQ Orbitrap Discoverer mass spectrometer equipped with a nanospray ion source. Glycans are detected as singly [M + Na] + , doubly [M + Na] 2+ and triply [M + Na] 3+ charged species. Structural assignments are based on MS/MS fragmentation and known biosynthetic limitation 38 39 . (C) The relative abundance of Neu5Ac on the platelet PM of SAL-infused mice was 2.9-fold higher than Neu5Gc containing glycans, whereas the ratio for platelet PM of 5-HT-infused mice was 3-fold higher in Neu5Gc-containing glycans than Neu5Ac. The total Neu5Ac and Neu5Gc-containing glycans was not different between platelet PM of SAL and 5-HT mice.

Techniques Used: Mass Spectrometry, Mouse Assay, Isolation

Related Articles

Flow Cytometry:

Article Title: Radical new paradigm for heme degradation in Escherichia coli O157:H7
Article Snippet: .. The eluent was diluted in methanol and analyzed using NSI-MS by direct infusion into a linear ion trap mass spectrometer (LTQ-Orbitrap Discovery; Thermo Fisher Scientific) using a nanoelectrospray source at a syringe flow rate of 0.40 μL/min and capillary temperature set to 210 °C. .. Fragmentation by collision-induced dissociation (CID) in MS/MS was used with normalized collision energy of 35–40%.

Liquid Chromatography:

Article Title: The presence and role of bacterial quorum sensing in activated sludge
Article Snippet: .. N ‐acyl‐l ‐homoserine lactones were analysed with a LTQ Orbitrap XL hybrid Fourier Transform Mass Spectrometer (Thermo Fisher Scientific) equipped with static nanospray ion source for NSI‐MS and electrospray ionization source connected to an Accela liquid chromatography (LC) system (Thermo Fischer Scientific) for ESI‐LC‐MS. .. Volumes of 3 μl of standards and extracts were loaded into the nano‐electrospray needle and the voltage adjusted to 1.0 V. Signals were recorded for a period of 60 s. For LC‐ESI‐MS analysis, 25 μl of standards and extracts were chromatographed with a Phenomenex reversed‐phase C18 (150 × 2 mm × 5 μm) column.

Mass Spectrometry:

Article Title: A serotonin-induced N-glycan switch regulates platelet aggregation
Article Snippet: .. Nanospray ionization-linear ion trap mass spectrometry Mass analysis by NSI-MS was performed on a LTQ Orbitrap mass spectrometer (Thermo Scientific) equipped with a nanospray ion source. .. Briefly, permethylated glycans were dissolved in 1 mM NaOH in 50% methanol and infused directly into a linear ion trap mass spectrometer using a nanospray source at a syringe flow rate of 0.5 μl/min.

Article Title: Radical new paradigm for heme degradation in Escherichia coli O157:H7
Article Snippet: .. The eluent was diluted in methanol and analyzed using NSI-MS by direct infusion into a linear ion trap mass spectrometer (LTQ-Orbitrap Discovery; Thermo Fisher Scientific) using a nanoelectrospray source at a syringe flow rate of 0.40 μL/min and capillary temperature set to 210 °C. .. Fragmentation by collision-induced dissociation (CID) in MS/MS was used with normalized collision energy of 35–40%.

Article Title: The presence and role of bacterial quorum sensing in activated sludge
Article Snippet: .. N ‐acyl‐l ‐homoserine lactones were analysed with a LTQ Orbitrap XL hybrid Fourier Transform Mass Spectrometer (Thermo Fisher Scientific) equipped with static nanospray ion source for NSI‐MS and electrospray ionization source connected to an Accela liquid chromatography (LC) system (Thermo Fischer Scientific) for ESI‐LC‐MS. .. Volumes of 3 μl of standards and extracts were loaded into the nano‐electrospray needle and the voltage adjusted to 1.0 V. Signals were recorded for a period of 60 s. For LC‐ESI‐MS analysis, 25 μl of standards and extracts were chromatographed with a Phenomenex reversed‐phase C18 (150 × 2 mm × 5 μm) column.

Article Title: Radical new paradigm for heme degradation in Escherichia coli O157:H7
Article Snippet: .. NSI-MS was performed using a linear ion trap mass spectrometer (LTQ-Orbitrap Discovery; Thermo Fischer Scientific). .. Unless stated otherwise, all experimental procedures were performed in an anaerobic chamber under positive nitrogen:hydrogen (95:5) pressure with continuous scrubbing to remove any molecular oxygen.

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    Thermo Fisher lc nsi hrms ms
    Chromatograms obtained upon <t>LC-NSI-HRMS/MS</t> analysis of human leukocyte DNA (129 μg, 12.9 μg on column) containing 59.4 fmol N7-ethyl-Gua /μmol Gua. The relatively higher amount of analyte in this sample allowed for the confirmation
    Lc Nsi Hrms Ms, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher nanolc nsi ms ms analysis
    Representative <t>NanoLC-NSI/MS</t> 2 results for the quantifications of εdA ( A ), εdG ( B ), S -cdA ( C ), and S -cdG ( D ) in DNA of rat tissues. Shown are the selected-ion chromatograms (SICs) for monitoring the indicated MRM transitions for the analytes
    Nanolc Nsi Ms Ms Analysis, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Thermo Fisher nsi ms
    <t>NSI-MS</t> spectra of permethylated N -glycans from platelets of saline (SAL) (A) and 5-HT-infused (B) mice. An equal number of platelets from WT mice infused for 24 hr with SAL or 5-HT were collected and plasma membrane (PM) was isolated 34 . The N -linked glycans on membrane vesicles were released enzymatically by PNGase F. Released N -glycans were permethylated and profiled by an LTQ Orbitrap Discoverer mass spectrometer equipped with a <t>nanospray</t> ion source. Glycans are detected as singly [M + Na] + , doubly [M + Na] 2+ and triply [M + Na] 3+ charged species. Structural assignments are based on MS/MS fragmentation and known biosynthetic limitation 38 39 . (C) The relative abundance of Neu5Ac on the platelet PM of SAL-infused mice was 2.9-fold higher than Neu5Gc containing glycans, whereas the ratio for platelet PM of 5-HT-infused mice was 3-fold higher in Neu5Gc-containing glycans than Neu5Ac. The total Neu5Ac and Neu5Gc-containing glycans was not different between platelet PM of SAL and 5-HT mice.
    Nsi Ms, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nsi ms/product/Thermo Fisher
    Average 85 stars, based on 4 article reviews
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    92
    Thermo Fisher nanohplc nsi ms ms procedure
    Representative <t>nanoHPLC-NSI</t> + -MS/MS trace of dT-Tyr observed in rat cardiomyocytes following LAD ligation/reperfusion surgery (A) and quantitative data for dT-Tyr adducts formed in rat cardiomyocytes after LAD ligation/reperfusion or sham surgery (B).
    Nanohplc Nsi Ms Ms Procedure, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Chromatograms obtained upon LC-NSI-HRMS/MS analysis of human leukocyte DNA (129 μg, 12.9 μg on column) containing 59.4 fmol N7-ethyl-Gua /μmol Gua. The relatively higher amount of analyte in this sample allowed for the confirmation

    Journal: Chemical reviews

    Article Title: Mass Spectrometry of Structurally Modified DNA

    doi: 10.1021/cr300391r

    Figure Lengend Snippet: Chromatograms obtained upon LC-NSI-HRMS/MS analysis of human leukocyte DNA (129 μg, 12.9 μg on column) containing 59.4 fmol N7-ethyl-Gua /μmol Gua. The relatively higher amount of analyte in this sample allowed for the confirmation

    Article Snippet: The LC-NSI-HRMS/MS method used LTQ Orbitrap Velos instrument (Thermo Scientific, Waltham, MA) and nanospray source (Thermo Scientific) and a Nano2D-LC HPLC (Eksigent, Dublin, CA).

    Techniques: Mass Spectrometry

    Representative NanoLC-NSI/MS 2 results for the quantifications of εdA ( A ), εdG ( B ), S -cdA ( C ), and S -cdG ( D ) in DNA of rat tissues. Shown are the selected-ion chromatograms (SICs) for monitoring the indicated MRM transitions for the analytes

    Journal: Molecular & Cellular Proteomics : MCP

    Article Title: Comprehensive Assessment of Oxidatively Induced Modifications of DNA in a Rat Model of Human Wilson's Disease *

    doi: 10.1074/mcp.M115.052696

    Figure Lengend Snippet: Representative NanoLC-NSI/MS 2 results for the quantifications of εdA ( A ), εdG ( B ), S -cdA ( C ), and S -cdG ( D ) in DNA of rat tissues. Shown are the selected-ion chromatograms (SICs) for monitoring the indicated MRM transitions for the analytes

    Article Snippet: The NanoLC-NSI-MS/MS analysis was conducted on a TSQ Vantage triple quadrupole mass spectrometer (Thermo Fisher Scientific) equipped with a nano-electrospray ionization source and coupled with an EASY nLC II (Thermo Fisher Scientific).

    Techniques: Mass Spectrometry

    NSI-MS spectra of permethylated N -glycans from platelets of saline (SAL) (A) and 5-HT-infused (B) mice. An equal number of platelets from WT mice infused for 24 hr with SAL or 5-HT were collected and plasma membrane (PM) was isolated 34 . The N -linked glycans on membrane vesicles were released enzymatically by PNGase F. Released N -glycans were permethylated and profiled by an LTQ Orbitrap Discoverer mass spectrometer equipped with a nanospray ion source. Glycans are detected as singly [M + Na] + , doubly [M + Na] 2+ and triply [M + Na] 3+ charged species. Structural assignments are based on MS/MS fragmentation and known biosynthetic limitation 38 39 . (C) The relative abundance of Neu5Ac on the platelet PM of SAL-infused mice was 2.9-fold higher than Neu5Gc containing glycans, whereas the ratio for platelet PM of 5-HT-infused mice was 3-fold higher in Neu5Gc-containing glycans than Neu5Ac. The total Neu5Ac and Neu5Gc-containing glycans was not different between platelet PM of SAL and 5-HT mice.

    Journal: Scientific Reports

    Article Title: A serotonin-induced N-glycan switch regulates platelet aggregation

    doi: 10.1038/srep02795

    Figure Lengend Snippet: NSI-MS spectra of permethylated N -glycans from platelets of saline (SAL) (A) and 5-HT-infused (B) mice. An equal number of platelets from WT mice infused for 24 hr with SAL or 5-HT were collected and plasma membrane (PM) was isolated 34 . The N -linked glycans on membrane vesicles were released enzymatically by PNGase F. Released N -glycans were permethylated and profiled by an LTQ Orbitrap Discoverer mass spectrometer equipped with a nanospray ion source. Glycans are detected as singly [M + Na] + , doubly [M + Na] 2+ and triply [M + Na] 3+ charged species. Structural assignments are based on MS/MS fragmentation and known biosynthetic limitation 38 39 . (C) The relative abundance of Neu5Ac on the platelet PM of SAL-infused mice was 2.9-fold higher than Neu5Gc containing glycans, whereas the ratio for platelet PM of 5-HT-infused mice was 3-fold higher in Neu5Gc-containing glycans than Neu5Ac. The total Neu5Ac and Neu5Gc-containing glycans was not different between platelet PM of SAL and 5-HT mice.

    Article Snippet: Nanospray ionization-linear ion trap mass spectrometry Mass analysis by NSI-MS was performed on a LTQ Orbitrap mass spectrometer (Thermo Scientific) equipped with a nanospray ion source.

    Techniques: Mass Spectrometry, Mouse Assay, Isolation

    Representative nanoHPLC-NSI + -MS/MS trace of dT-Tyr observed in rat cardiomyocytes following LAD ligation/reperfusion surgery (A) and quantitative data for dT-Tyr adducts formed in rat cardiomyocytes after LAD ligation/reperfusion or sham surgery (B).

    Journal: Free radical biology & medicine

    Article Title: Oxidative Cross-Linking of Proteins to DNA Following Ischemia-Reperfusion Injury

    doi: 10.1016/j.freeradbiomed.2018.03.010

    Figure Lengend Snippet: Representative nanoHPLC-NSI + -MS/MS trace of dT-Tyr observed in rat cardiomyocytes following LAD ligation/reperfusion surgery (A) and quantitative data for dT-Tyr adducts formed in rat cardiomyocytes after LAD ligation/reperfusion or sham surgery (B).

    Article Snippet: We thank Aimee Rinas (Thermo Fischer Scientific) for her contribution to the development of the FASP procedure, TMT labeling procedure, and assisting with nanoLC-NSI-MS/MS analysis, Rosa Viner (Thermo Fischer Scientific) for her help in the development of the nanoHPLC-NSI+ -MS/MS procedure for analyzing TMT-labeled peptides, Ryan Bomgarden (Thermo Fischer Scientific) for his contributions to the DPC sample preparation procedure for TMT labeleing, and Robert Carlson (University of Minnesota) for creating the graphics for this article.

    Techniques: Mass Spectrometry, Ligation