trpm7 inhibitor ns 8593  (Alomone Labs)


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    Alomone Labs trpm7 inhibitor ns 8593
    Trpm7 Inhibitor Ns 8593, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/trpm7 inhibitor ns 8593/product/Alomone Labs
    Average 92 stars, based on 1 article reviews
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    trpm7 inhibitor ns 8593 - by Bioz Stars, 2023-03
    92/100 stars

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    ns 8593  (Alomone Labs)


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    Alomone Labs ns 8593
    Rat BMVEC in vitro BBB model. Passage 5 isolated rBMVECs were seeded into Corning transwell plate inserts and grown for three days, to allow a barrier to form as confirmed by Transendothelial Electrical Resistance (TEER) measurements. Media only was added to wells to account for background. BMVECs were treated with either 0 or 52.2 mM EtOH, as well as TRPM7 antagonists, NS 8593, CyPPA, or SKA31 in both 0 and 52.2 mM EtOH treatment conditions. Ten μg/mL of Sodium Fluorescein (NaF) was added to each insert for permeability assessment prior to assay incubation at 37 °C for 24 h. Permeability coefficient was calculated, along with p -value by Student’s t test. * p < 0.05, ** p < 0.01.
    Ns 8593, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ns 8593/product/Alomone Labs
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ns 8593 - by Bioz Stars, 2023-03
    86/100 stars

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    1) Product Images from "Involvement of TRPM7 in Alcohol-Induced Damage of the Blood–Brain Barrier in the Presence of HIV Viral Proteins"

    Article Title: Involvement of TRPM7 in Alcohol-Induced Damage of the Blood–Brain Barrier in the Presence of HIV Viral Proteins

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms24031910

    Rat BMVEC in vitro BBB model. Passage 5 isolated rBMVECs were seeded into Corning transwell plate inserts and grown for three days, to allow a barrier to form as confirmed by Transendothelial Electrical Resistance (TEER) measurements. Media only was added to wells to account for background. BMVECs were treated with either 0 or 52.2 mM EtOH, as well as TRPM7 antagonists, NS 8593, CyPPA, or SKA31 in both 0 and 52.2 mM EtOH treatment conditions. Ten μg/mL of Sodium Fluorescein (NaF) was added to each insert for permeability assessment prior to assay incubation at 37 °C for 24 h. Permeability coefficient was calculated, along with p -value by Student’s t test. * p < 0.05, ** p < 0.01.
    Figure Legend Snippet: Rat BMVEC in vitro BBB model. Passage 5 isolated rBMVECs were seeded into Corning transwell plate inserts and grown for three days, to allow a barrier to form as confirmed by Transendothelial Electrical Resistance (TEER) measurements. Media only was added to wells to account for background. BMVECs were treated with either 0 or 52.2 mM EtOH, as well as TRPM7 antagonists, NS 8593, CyPPA, or SKA31 in both 0 and 52.2 mM EtOH treatment conditions. Ten μg/mL of Sodium Fluorescein (NaF) was added to each insert for permeability assessment prior to assay incubation at 37 °C for 24 h. Permeability coefficient was calculated, along with p -value by Student’s t test. * p < 0.05, ** p < 0.01.

    Techniques Used: In Vitro, Isolation, Permeability, Incubation

    trpm7 inhibitor ns 8593  (Alomone Labs)


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    Alomone Labs trpm7 inhibitor ns 8593
    Trpm7 Inhibitor Ns 8593, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/trpm7 inhibitor ns 8593/product/Alomone Labs
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    trpm7 inhibitor ns 8593 - by Bioz Stars, 2023-03
    92/100 stars

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    ns 8593  (Alomone Labs)


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    Alomone Labs ns 8593
    Effect of TRPM7 inhibitor <t>NS-8593</t> on oocyte activation. a [Ca2+]i oscillations of NS-8593-inhibited oocytes. b Mitochondrial membrane potential dynamic changes of 1 μM NS-8593-inhibited oocytes. c Mitochondrial membrane potential fluorescence intensity of 1 μM NS-8593-inhibited oocytes. The green and red curves represent low and high membrane potential, respectively. The black curve shows the ratio of high membrane potential to low membrane potential indicating relative mitochondrial membrane potential. d Living cell observation of 1 μM NS-8593 inhibition of oocyte spindle formation e Living cell observation of 1 μM NS-8593 inhibition of oocyte subcortical F-actin distribution
    Ns 8593, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ns 8593/product/Alomone Labs
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ns 8593 - by Bioz Stars, 2023-03
    92/100 stars

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    1) Product Images from "Regulation of [Ca 2+ ] i oscillations and mitochondrial activity by various calcium transporters in mouse oocytes"

    Article Title: Regulation of [Ca 2+ ] i oscillations and mitochondrial activity by various calcium transporters in mouse oocytes

    Journal: Reproductive Biology and Endocrinology : RB&E

    doi: 10.1186/s12958-020-00643-7

    Effect of TRPM7 inhibitor NS-8593 on oocyte activation. a [Ca2+]i oscillations of NS-8593-inhibited oocytes. b Mitochondrial membrane potential dynamic changes of 1 μM NS-8593-inhibited oocytes. c Mitochondrial membrane potential fluorescence intensity of 1 μM NS-8593-inhibited oocytes. The green and red curves represent low and high membrane potential, respectively. The black curve shows the ratio of high membrane potential to low membrane potential indicating relative mitochondrial membrane potential. d Living cell observation of 1 μM NS-8593 inhibition of oocyte spindle formation e Living cell observation of 1 μM NS-8593 inhibition of oocyte subcortical F-actin distribution
    Figure Legend Snippet: Effect of TRPM7 inhibitor NS-8593 on oocyte activation. a [Ca2+]i oscillations of NS-8593-inhibited oocytes. b Mitochondrial membrane potential dynamic changes of 1 μM NS-8593-inhibited oocytes. c Mitochondrial membrane potential fluorescence intensity of 1 μM NS-8593-inhibited oocytes. The green and red curves represent low and high membrane potential, respectively. The black curve shows the ratio of high membrane potential to low membrane potential indicating relative mitochondrial membrane potential. d Living cell observation of 1 μM NS-8593 inhibition of oocyte spindle formation e Living cell observation of 1 μM NS-8593 inhibition of oocyte subcortical F-actin distribution

    Techniques Used: Activation Assay, Fluorescence, Inhibition

    Inhibitor effects on on long-lasting [Ca 2+ ] i oscillations. a Cytoplasmic [Ca 2+ ] i dynamic changes after inhibitor addition. b Development of inhibitor addition following [Ca 2+ ] i oscillations initiation. PA indicates survival of embryos of all MII oocytes. PN indicates pronuclear embryos of surviving oocytes. 2-Cell indicates cleaved embryos of pronuclear embryos. RR: 10 μM Ruthenium Red; Tha: 1 μM Thapsigargin; GSK: 1 mM GSK-7975A; Mib: Mibefradil; N: 1 μM NS-8593. The significance of differences between groups was analyzed by the Chi-square test and p < 0.05 (*) was considered statistically significant. (X) indicates data unavailable
    Figure Legend Snippet: Inhibitor effects on on long-lasting [Ca 2+ ] i oscillations. a Cytoplasmic [Ca 2+ ] i dynamic changes after inhibitor addition. b Development of inhibitor addition following [Ca 2+ ] i oscillations initiation. PA indicates survival of embryos of all MII oocytes. PN indicates pronuclear embryos of surviving oocytes. 2-Cell indicates cleaved embryos of pronuclear embryos. RR: 10 μM Ruthenium Red; Tha: 1 μM Thapsigargin; GSK: 1 mM GSK-7975A; Mib: Mibefradil; N: 1 μM NS-8593. The significance of differences between groups was analyzed by the Chi-square test and p < 0.05 (*) was considered statistically significant. (X) indicates data unavailable

    Techniques Used:

    ns 8593  (Alomone Labs)


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    Alomone Labs ns 8593
    Ns 8593, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ns 8593/product/Alomone Labs
    Average 92 stars, based on 1 article reviews
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    ns 8593 - by Bioz Stars, 2023-03
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    ns 8593  (Alomone Labs)


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    Alomone Labs ns 8593
    Endothelial cell barrier permeability following treatment with a) different concentrations of EtOH or b) the TRPM7 antagonists, NS8593, CyPPA, or SKA-31. *p < 0.05; **p < 0.01. a Mouse BMVECs at Passage 7 were seeded into Corning transwell inserts seated in 24-well plates to construct an in-vitro Blood-Brain Barrier (BBB) model. The cells were allowed to grow in the inserts for three days to form a barrier. The same number of wells without cells were used as background. The wells were then treated with 0 mM, 17.4 mM, 52.2 mM and 104.4 mM EtOH for 24 h, and to determine permeability, 50 ng/μl Evans Blue Dye (EBD) was added into the inserts at the same time and the model was incubated at 37 °C for 24 h. The permeability coefficient was then calculated. P-value was calculated using student’s t test. b Mouse BMVECs at Passage 5 were seeded into Corning transwell inserts seated in 24-well plates to construct an in-vitro Blood-Brain Barrier (BBB) model. The cells were allowed to grow in the inserts for three days to form a barrier (BBB). Wells were then treated with three different Trpm 7 Channel Blockers, NS 8593, CyPPA and SKA-31 at 50 μM for 24 h, and the permeability assay was then performed to determine the effect of TRPM7 blockers on the BBB. P-values were calculated using student’s t test
    Ns 8593, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ns 8593/product/Alomone Labs
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ns 8593 - by Bioz Stars, 2023-03
    86/100 stars

    Images

    1) Product Images from "Ethanol’s Effects on Transient Receptor Potential Channel Expression in Brain Microvascular Endothelial Cells"

    Article Title: Ethanol’s Effects on Transient Receptor Potential Channel Expression in Brain Microvascular Endothelial Cells

    Journal: Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology

    doi: 10.1007/s11481-018-9796-3

    Endothelial cell barrier permeability following treatment with a) different concentrations of EtOH or b) the TRPM7 antagonists, NS8593, CyPPA, or SKA-31. *p < 0.05; **p < 0.01. a Mouse BMVECs at Passage 7 were seeded into Corning transwell inserts seated in 24-well plates to construct an in-vitro Blood-Brain Barrier (BBB) model. The cells were allowed to grow in the inserts for three days to form a barrier. The same number of wells without cells were used as background. The wells were then treated with 0 mM, 17.4 mM, 52.2 mM and 104.4 mM EtOH for 24 h, and to determine permeability, 50 ng/μl Evans Blue Dye (EBD) was added into the inserts at the same time and the model was incubated at 37 °C for 24 h. The permeability coefficient was then calculated. P-value was calculated using student’s t test. b Mouse BMVECs at Passage 5 were seeded into Corning transwell inserts seated in 24-well plates to construct an in-vitro Blood-Brain Barrier (BBB) model. The cells were allowed to grow in the inserts for three days to form a barrier (BBB). Wells were then treated with three different Trpm 7 Channel Blockers, NS 8593, CyPPA and SKA-31 at 50 μM for 24 h, and the permeability assay was then performed to determine the effect of TRPM7 blockers on the BBB. P-values were calculated using student’s t test
    Figure Legend Snippet: Endothelial cell barrier permeability following treatment with a) different concentrations of EtOH or b) the TRPM7 antagonists, NS8593, CyPPA, or SKA-31. *p < 0.05; **p < 0.01. a Mouse BMVECs at Passage 7 were seeded into Corning transwell inserts seated in 24-well plates to construct an in-vitro Blood-Brain Barrier (BBB) model. The cells were allowed to grow in the inserts for three days to form a barrier. The same number of wells without cells were used as background. The wells were then treated with 0 mM, 17.4 mM, 52.2 mM and 104.4 mM EtOH for 24 h, and to determine permeability, 50 ng/μl Evans Blue Dye (EBD) was added into the inserts at the same time and the model was incubated at 37 °C for 24 h. The permeability coefficient was then calculated. P-value was calculated using student’s t test. b Mouse BMVECs at Passage 5 were seeded into Corning transwell inserts seated in 24-well plates to construct an in-vitro Blood-Brain Barrier (BBB) model. The cells were allowed to grow in the inserts for three days to form a barrier (BBB). Wells were then treated with three different Trpm 7 Channel Blockers, NS 8593, CyPPA and SKA-31 at 50 μM for 24 h, and the permeability assay was then performed to determine the effect of TRPM7 blockers on the BBB. P-values were calculated using student’s t test

    Techniques Used: Permeability, Construct, In Vitro, Incubation

    ns 8593  (Alomone Labs)


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    Alomone Labs ns 8593
    Endothelial cell barrier permeability following treatment with a) different concentrations of EtOH or b) the TRPM7 antagonists, NS8593, CyPPA, or SKA-31. *p < 0.05; **p < 0.01. a Mouse BMVECs at Passage 7 were seeded into Corning transwell inserts seated in 24-well plates to construct an in-vitro Blood-Brain Barrier (BBB) model. The cells were allowed to grow in the inserts for three days to form a barrier. The same number of wells without cells were used as background. The wells were then treated with 0 mM, 17.4 mM, 52.2 mM and 104.4 mM EtOH for 24 h, and to determine permeability, 50 ng/μl Evans Blue Dye (EBD) was added into the inserts at the same time and the model was incubated at 37 °C for 24 h. The permeability coefficient was then calculated. P-value was calculated using student’s t test. b Mouse BMVECs at Passage 5 were seeded into Corning transwell inserts seated in 24-well plates to construct an in-vitro Blood-Brain Barrier (BBB) model. The cells were allowed to grow in the inserts for three days to form a barrier (BBB). Wells were then treated with three different Trpm 7 Channel Blockers, NS 8593, CyPPA and SKA-31 at 50 μM for 24 h, and the permeability assay was then performed to determine the effect of TRPM7 blockers on the BBB. P-values were calculated using student’s t test
    Ns 8593, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ns 8593/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ns 8593 - by Bioz Stars, 2023-03
    93/100 stars

    Images

    1) Product Images from "Ethanol’s Effects on Transient Receptor Potential Channel Expression in Brain Microvascular Endothelial Cells"

    Article Title: Ethanol’s Effects on Transient Receptor Potential Channel Expression in Brain Microvascular Endothelial Cells

    Journal: Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology

    doi: 10.1007/s11481-018-9796-3

    Endothelial cell barrier permeability following treatment with a) different concentrations of EtOH or b) the TRPM7 antagonists, NS8593, CyPPA, or SKA-31. *p < 0.05; **p < 0.01. a Mouse BMVECs at Passage 7 were seeded into Corning transwell inserts seated in 24-well plates to construct an in-vitro Blood-Brain Barrier (BBB) model. The cells were allowed to grow in the inserts for three days to form a barrier. The same number of wells without cells were used as background. The wells were then treated with 0 mM, 17.4 mM, 52.2 mM and 104.4 mM EtOH for 24 h, and to determine permeability, 50 ng/μl Evans Blue Dye (EBD) was added into the inserts at the same time and the model was incubated at 37 °C for 24 h. The permeability coefficient was then calculated. P-value was calculated using student’s t test. b Mouse BMVECs at Passage 5 were seeded into Corning transwell inserts seated in 24-well plates to construct an in-vitro Blood-Brain Barrier (BBB) model. The cells were allowed to grow in the inserts for three days to form a barrier (BBB). Wells were then treated with three different Trpm 7 Channel Blockers, NS 8593, CyPPA and SKA-31 at 50 μM for 24 h, and the permeability assay was then performed to determine the effect of TRPM7 blockers on the BBB. P-values were calculated using student’s t test
    Figure Legend Snippet: Endothelial cell barrier permeability following treatment with a) different concentrations of EtOH or b) the TRPM7 antagonists, NS8593, CyPPA, or SKA-31. *p < 0.05; **p < 0.01. a Mouse BMVECs at Passage 7 were seeded into Corning transwell inserts seated in 24-well plates to construct an in-vitro Blood-Brain Barrier (BBB) model. The cells were allowed to grow in the inserts for three days to form a barrier. The same number of wells without cells were used as background. The wells were then treated with 0 mM, 17.4 mM, 52.2 mM and 104.4 mM EtOH for 24 h, and to determine permeability, 50 ng/μl Evans Blue Dye (EBD) was added into the inserts at the same time and the model was incubated at 37 °C for 24 h. The permeability coefficient was then calculated. P-value was calculated using student’s t test. b Mouse BMVECs at Passage 5 were seeded into Corning transwell inserts seated in 24-well plates to construct an in-vitro Blood-Brain Barrier (BBB) model. The cells were allowed to grow in the inserts for three days to form a barrier (BBB). Wells were then treated with three different Trpm 7 Channel Blockers, NS 8593, CyPPA and SKA-31 at 50 μM for 24 h, and the permeability assay was then performed to determine the effect of TRPM7 blockers on the BBB. P-values were calculated using student’s t test

    Techniques Used: Permeability, Construct, In Vitro, Incubation

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    Alomone Labs trpm7 inhibitor ns 8593
    Trpm7 Inhibitor Ns 8593, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/trpm7 inhibitor ns 8593/product/Alomone Labs
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    trpm7 inhibitor ns 8593 - by Bioz Stars, 2023-03
    92/100 stars
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    ns 8593  (Alomone Labs)
    86
    Alomone Labs ns 8593
    Rat BMVEC in vitro BBB model. Passage 5 isolated rBMVECs were seeded into Corning transwell plate inserts and grown for three days, to allow a barrier to form as confirmed by Transendothelial Electrical Resistance (TEER) measurements. Media only was added to wells to account for background. BMVECs were treated with either 0 or 52.2 mM EtOH, as well as TRPM7 antagonists, NS 8593, CyPPA, or SKA31 in both 0 and 52.2 mM EtOH treatment conditions. Ten μg/mL of Sodium Fluorescein (NaF) was added to each insert for permeability assessment prior to assay incubation at 37 °C for 24 h. Permeability coefficient was calculated, along with p -value by Student’s t test. * p < 0.05, ** p < 0.01.
    Ns 8593, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ns 8593/product/Alomone Labs
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ns 8593 - by Bioz Stars, 2023-03
    86/100 stars
    		  Buy from Supplier

    Image Search Results


    Rat BMVEC in vitro BBB model. Passage 5 isolated rBMVECs were seeded into Corning transwell plate inserts and grown for three days, to allow a barrier to form as confirmed by Transendothelial Electrical Resistance (TEER) measurements. Media only was added to wells to account for background. BMVECs were treated with either 0 or 52.2 mM EtOH, as well as TRPM7 antagonists, NS 8593, CyPPA, or SKA31 in both 0 and 52.2 mM EtOH treatment conditions. Ten μg/mL of Sodium Fluorescein (NaF) was added to each insert for permeability assessment prior to assay incubation at 37 °C for 24 h. Permeability coefficient was calculated, along with p -value by Student’s t test. * p < 0.05, ** p < 0.01.

    Journal: International Journal of Molecular Sciences

    Article Title: Involvement of TRPM7 in Alcohol-Induced Damage of the Blood–Brain Barrier in the Presence of HIV Viral Proteins

    doi: 10.3390/ijms24031910

    Figure Lengend Snippet: Rat BMVEC in vitro BBB model. Passage 5 isolated rBMVECs were seeded into Corning transwell plate inserts and grown for three days, to allow a barrier to form as confirmed by Transendothelial Electrical Resistance (TEER) measurements. Media only was added to wells to account for background. BMVECs were treated with either 0 or 52.2 mM EtOH, as well as TRPM7 antagonists, NS 8593, CyPPA, or SKA31 in both 0 and 52.2 mM EtOH treatment conditions. Ten μg/mL of Sodium Fluorescein (NaF) was added to each insert for permeability assessment prior to assay incubation at 37 °C for 24 h. Permeability coefficient was calculated, along with p -value by Student’s t test. * p < 0.05, ** p < 0.01.

    Article Snippet: HIV-1 viral protein, gp120 (NIH-ARP, 4961) and three different TRPM7 Channel Blockers, NS 8593, CyPPA and SKA-31 (Alomone Labs, Jerusalem, Israel) were added to inserts and bottom of the wells only.

    Techniques: In Vitro, Isolation, Permeability, Incubation