Structured Review

OriGene nrac open reading frame
<t>Nrac</t> localizes to the plasma membrane. A) Predicted transmembrane domains. Fluorescence imaging of HEK293 cells transfected with plasmids encoding B) LCK-tRFP and C) <t>Nrac-GFP</t> and D) Merged picture. E) Immunostaining with a primary antibody against Nrac, visualized by an Alexa Fluor 488-conjugated secondary antibody, F) Hoechst staining for nuclei, and G) merged picture, in paraffin-embedded sections of mouse white adipose tissue.
Nrac Open Reading Frame, supplied by OriGene, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/nrac open reading frame/product/OriGene
Average 85 stars, based on 1 article reviews
Price from $9.99 to $1999.99
nrac open reading frame - by Bioz Stars, 2020-09
85/100 stars

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1) Product Images from "Nrac, a Novel Nutritionally-Regulated Adipose and Cardiac-Enriched Gene"

Article Title: Nrac, a Novel Nutritionally-Regulated Adipose and Cardiac-Enriched Gene

Journal: PLoS ONE

doi: 10.1371/journal.pone.0046254

Nrac localizes to the plasma membrane. A) Predicted transmembrane domains. Fluorescence imaging of HEK293 cells transfected with plasmids encoding B) LCK-tRFP and C) Nrac-GFP and D) Merged picture. E) Immunostaining with a primary antibody against Nrac, visualized by an Alexa Fluor 488-conjugated secondary antibody, F) Hoechst staining for nuclei, and G) merged picture, in paraffin-embedded sections of mouse white adipose tissue.
Figure Legend Snippet: Nrac localizes to the plasma membrane. A) Predicted transmembrane domains. Fluorescence imaging of HEK293 cells transfected with plasmids encoding B) LCK-tRFP and C) Nrac-GFP and D) Merged picture. E) Immunostaining with a primary antibody against Nrac, visualized by an Alexa Fluor 488-conjugated secondary antibody, F) Hoechst staining for nuclei, and G) merged picture, in paraffin-embedded sections of mouse white adipose tissue.

Techniques Used: Fluorescence, Imaging, Transfection, Immunostaining, Staining

Related Articles

Clone Assay:

Article Title: Nrac, a Novel Nutritionally-Regulated Adipose and Cardiac-Enriched Gene
Article Snippet: .. To examine Nrac subcellular localization, fusion protein of Nrac-GFP was made by cloning the Nrac open reading frame (Origene, MD) into the pCMV6-AC-GFP vector. .. In HEK293 cells, the Nrac-GFP vector was cotransfected with a pCMV6 vector that encodes a fusion protein of LCK (lymphocyte-specific protein tyrosine kinase) and red fluorescence protein (Origene, MD).

Plasmid Preparation:

Article Title: Nrac, a Novel Nutritionally-Regulated Adipose and Cardiac-Enriched Gene
Article Snippet: .. To examine Nrac subcellular localization, fusion protein of Nrac-GFP was made by cloning the Nrac open reading frame (Origene, MD) into the pCMV6-AC-GFP vector. .. In HEK293 cells, the Nrac-GFP vector was cotransfected with a pCMV6 vector that encodes a fusion protein of LCK (lymphocyte-specific protein tyrosine kinase) and red fluorescence protein (Origene, MD).

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    OriGene nrac open reading frame
    <t>Nrac</t> localizes to the plasma membrane. A) Predicted transmembrane domains. Fluorescence imaging of HEK293 cells transfected with plasmids encoding B) LCK-tRFP and C) <t>Nrac-GFP</t> and D) Merged picture. E) Immunostaining with a primary antibody against Nrac, visualized by an Alexa Fluor 488-conjugated secondary antibody, F) Hoechst staining for nuclei, and G) merged picture, in paraffin-embedded sections of mouse white adipose tissue.
    Nrac Open Reading Frame, supplied by OriGene, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nrac open reading frame/product/OriGene
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    nrac open reading frame - by Bioz Stars, 2020-09
    85/100 stars
      Buy from Supplier

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    Nrac localizes to the plasma membrane. A) Predicted transmembrane domains. Fluorescence imaging of HEK293 cells transfected with plasmids encoding B) LCK-tRFP and C) Nrac-GFP and D) Merged picture. E) Immunostaining with a primary antibody against Nrac, visualized by an Alexa Fluor 488-conjugated secondary antibody, F) Hoechst staining for nuclei, and G) merged picture, in paraffin-embedded sections of mouse white adipose tissue.

    Journal: PLoS ONE

    Article Title: Nrac, a Novel Nutritionally-Regulated Adipose and Cardiac-Enriched Gene

    doi: 10.1371/journal.pone.0046254

    Figure Lengend Snippet: Nrac localizes to the plasma membrane. A) Predicted transmembrane domains. Fluorescence imaging of HEK293 cells transfected with plasmids encoding B) LCK-tRFP and C) Nrac-GFP and D) Merged picture. E) Immunostaining with a primary antibody against Nrac, visualized by an Alexa Fluor 488-conjugated secondary antibody, F) Hoechst staining for nuclei, and G) merged picture, in paraffin-embedded sections of mouse white adipose tissue.

    Article Snippet: To examine Nrac subcellular localization, fusion protein of Nrac-GFP was made by cloning the Nrac open reading frame (Origene, MD) into the pCMV6-AC-GFP vector.

    Techniques: Fluorescence, Imaging, Transfection, Immunostaining, Staining