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Thermo Fisher nr dtt
Nr Dtt, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 80 stars, based on 1 article reviews
Price from $9.99 to $1999.99
nr dtt - by Bioz Stars, 2020-03
80/100 stars

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Flow Cytometry:

Article Title: Induction of crossreactive antibodies against the Plasmodium falciparum variant protein
Article Snippet: Set points for fermentation were: pH 6.5; air flow rate, 2.5 liter/min; temperature, 30°C; dissolved oxygen, 30%; agitation, 360–1,000 rpm (cascaded with the dissolved oxygen). .. Proteins were separated by SDS/PAGE with (R) or without (NR) DTT at a final concentration of 50 mM (Invitrogen) on 4–20% gradient gels (Invitrogen) per the manufacturer's instructions.

Bicinchoninic Acid Protein Assay:

Article Title: Induction of crossreactive antibodies against the Plasmodium falciparum variant protein
Article Snippet: Proteins were separated by SDS/PAGE with (R) or without (NR) DTT at a final concentration of 50 mM (Invitrogen) on 4–20% gradient gels (Invitrogen) per the manufacturer's instructions. .. Proteins were separated by SDS/PAGE with (R) or without (NR) DTT at a final concentration of 50 mM (Invitrogen) on 4–20% gradient gels (Invitrogen) per the manufacturer's instructions.

Size-exclusion Chromatography:

Article Title: Induction of crossreactive antibodies against the Plasmodium falciparum variant protein
Article Snippet: The protein was purified by using nickel-nitrilotriacetic acid-agarose (Ni-NTA; Qiagen) followed by size-exclusion chromatography on a Superdex 75 column (Amersham Pharmacia). .. Proteins were separated by SDS/PAGE with (R) or without (NR) DTT at a final concentration of 50 mM (Invitrogen) on 4–20% gradient gels (Invitrogen) per the manufacturer's instructions.

Purification:

Article Title: Induction of crossreactive antibodies against the Plasmodium falciparum variant protein
Article Snippet: The protein was purified from the supernatant by using Ni-NTA agarose followed by an anion-exchange column (Hiload 16/10, Q Sepharose HP, Amersham Pharmacia) and by a cation-exchange column (Hiload 16/10, SP Sepharose HP). .. Proteins were separated by SDS/PAGE with (R) or without (NR) DTT at a final concentration of 50 mM (Invitrogen) on 4–20% gradient gels (Invitrogen) per the manufacturer's instructions.

Concentration Assay:

Article Title: Induction of crossreactive antibodies against the Plasmodium falciparum variant protein
Article Snippet: .. Proteins were separated by SDS/PAGE with (R) or without (NR) DTT at a final concentration of 50 mM (Invitrogen) on 4–20% gradient gels (Invitrogen) per the manufacturer's instructions. .. Gels were either stained with Coomassie brilliant blue or prepared for electrophoretic transfer to poly(vinylidene difluoride) (PVDF) membranes (Invitrogen).

Sequencing:

Article Title: Induction of crossreactive antibodies against the Plasmodium falciparum variant protein
Article Snippet: Proteins were separated by SDS/PAGE with (R) or without (NR) DTT at a final concentration of 50 mM (Invitrogen) on 4–20% gradient gels (Invitrogen) per the manufacturer's instructions. .. Samples bound to PVDF were subjected to amino acid sequencing by automated Edman degradation (performed at the Biological Resources Branch, National Institute of Allergy and Infectious Diseases, National Institutes of Health).

Staining:

Article Title: Induction of crossreactive antibodies against the Plasmodium falciparum variant protein
Article Snippet: Proteins were separated by SDS/PAGE with (R) or without (NR) DTT at a final concentration of 50 mM (Invitrogen) on 4–20% gradient gels (Invitrogen) per the manufacturer's instructions. .. Gels were either stained with Coomassie brilliant blue or prepared for electrophoretic transfer to poly(vinylidene difluoride) (PVDF) membranes (Invitrogen).

SDS Page:

Article Title: Induction of crossreactive antibodies against the Plasmodium falciparum variant protein
Article Snippet: .. Proteins were separated by SDS/PAGE with (R) or without (NR) DTT at a final concentration of 50 mM (Invitrogen) on 4–20% gradient gels (Invitrogen) per the manufacturer's instructions. .. Gels were either stained with Coomassie brilliant blue or prepared for electrophoretic transfer to poly(vinylidene difluoride) (PVDF) membranes (Invitrogen).

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    Thermo Fisher dtt
    The chemical structure and 1 H NMR spectrum of PSST polymer are shown in (A). The gel permeation chromatography (GPC) curves of PSST polymer treated with or without 10 <t>mmol/L</t> <t>DTT</t> for 6 h were determined (B). The critical micellar concentration (CMC) value of PSST polymer was determined by the change of pyrene fluorescence (C). The MTT assay was used to examine viability of A2780 and A2780/PTX cells treated with PSST polymer for 48 h (D).
    Dtt, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 2501 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dtt/product/Thermo Fisher
    Average 99 stars, based on 2501 article reviews
    Price from $9.99 to $1999.99
    dtt - by Bioz Stars, 2020-03
    99/100 stars
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    The chemical structure and 1 H NMR spectrum of PSST polymer are shown in (A). The gel permeation chromatography (GPC) curves of PSST polymer treated with or without 10 mmol/L DTT for 6 h were determined (B). The critical micellar concentration (CMC) value of PSST polymer was determined by the change of pyrene fluorescence (C). The MTT assay was used to examine viability of A2780 and A2780/PTX cells treated with PSST polymer for 48 h (D).

    Journal: Acta Pharmacologica Sinica

    Article Title: Reversal of paclitaxel resistance in human ovarian cancer cells with redox-responsive micelles consisting of α-tocopheryl succinate-based polyphosphoester copolymers

    doi: 10.1038/aps.2016.150

    Figure Lengend Snippet: The chemical structure and 1 H NMR spectrum of PSST polymer are shown in (A). The gel permeation chromatography (GPC) curves of PSST polymer treated with or without 10 mmol/L DTT for 6 h were determined (B). The critical micellar concentration (CMC) value of PSST polymer was determined by the change of pyrene fluorescence (C). The MTT assay was used to examine viability of A2780 and A2780/PTX cells treated with PSST polymer for 48 h (D).

    Article Snippet: The fluorescence emission of the amphiphilic aggregates loaded with NR in an environment with or without 10 mmol/L DTT was examined via fluorescence spectrophotometry (Thermo Fisher Scientific, Waltham, MA, USA) with excitation and emission wavelengths of 550 nm and 500–800 nm, respectively .

    Techniques: Nuclear Magnetic Resonance, GPC Assay, Gel Permeation Chromatography, Concentration Assay, Fluorescence, MTT Assay