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Abcam npm1 ma
INPP4B is upregulated by <t>NPM1-mA</t> in leukemia cells via ERK/Ets-1 signaling. ( a ) qRT-PCR analysis of INPP4B mRNA expression, ( b ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. ( c ) qRT-PCR analysis of INPP4B mRNA expression, ( d ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the THP-1 and K562 cells transduced with the plasmids expressing NPM1-wt or NPM1-mA. ( e ) qRT-PCR analysis of INPP4B mRNA expression, ( f ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3, p-Ets-1 and Ets-1 from the OCI-AML3 cells transfected with the control siRNA or siEts-1. ( g ) Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 and SGK3, ( h ) qRT-PCR analysis of INPP4B mRNA expression from the OCI-AML3 cells treated with different concentration of PD98059 (0, 10, 20 and 40 μM). i Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. Proteins were quantified using image software and normalized against β-actin. Data were represented as mean ± s.d. of three individual experiments. * p
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1) Product Images from "INPP4B promotes cell survival via SGK3 activation in NPM1-mutated leukemia"

Article Title: INPP4B promotes cell survival via SGK3 activation in NPM1-mutated leukemia

Journal: Journal of Experimental & Clinical Cancer Research : CR

doi: 10.1186/s13046-018-0675-9

INPP4B is upregulated by NPM1-mA in leukemia cells via ERK/Ets-1 signaling. ( a ) qRT-PCR analysis of INPP4B mRNA expression, ( b ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. ( c ) qRT-PCR analysis of INPP4B mRNA expression, ( d ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the THP-1 and K562 cells transduced with the plasmids expressing NPM1-wt or NPM1-mA. ( e ) qRT-PCR analysis of INPP4B mRNA expression, ( f ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3, p-Ets-1 and Ets-1 from the OCI-AML3 cells transfected with the control siRNA or siEts-1. ( g ) Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 and SGK3, ( h ) qRT-PCR analysis of INPP4B mRNA expression from the OCI-AML3 cells treated with different concentration of PD98059 (0, 10, 20 and 40 μM). i Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. Proteins were quantified using image software and normalized against β-actin. Data were represented as mean ± s.d. of three individual experiments. * p
Figure Legend Snippet: INPP4B is upregulated by NPM1-mA in leukemia cells via ERK/Ets-1 signaling. ( a ) qRT-PCR analysis of INPP4B mRNA expression, ( b ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. ( c ) qRT-PCR analysis of INPP4B mRNA expression, ( d ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the THP-1 and K562 cells transduced with the plasmids expressing NPM1-wt or NPM1-mA. ( e ) qRT-PCR analysis of INPP4B mRNA expression, ( f ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3, p-Ets-1 and Ets-1 from the OCI-AML3 cells transfected with the control siRNA or siEts-1. ( g ) Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 and SGK3, ( h ) qRT-PCR analysis of INPP4B mRNA expression from the OCI-AML3 cells treated with different concentration of PD98059 (0, 10, 20 and 40 μM). i Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. Proteins were quantified using image software and normalized against β-actin. Data were represented as mean ± s.d. of three individual experiments. * p

Techniques Used: Quantitative RT-PCR, Expressing, Western Blot, Transduction, Transfection, Concentration Assay, Software

NPM1-mA-mediated INPP4B upregulation promotes cell proliferation in OCI-AML3 cells. The NPM1-mA-silenced OCI-AML3 cells were subjected to ( a ) CCK8 assays and ( b ) colony forming assays. c The NPM1-mA-silenced OCI-AML3 cells were transfected with the pEAK-Flag/INPP4B plasmids, western blotting analysis of INPP4B and NPM1-mA. Proteins were quantified using image software and normalized against β-actin. d CCK-8 assay analysis of cell proliferation in NPM1-mA-silenced OCI-AML3 cells, followed by Flag-INPP4B introduction. Data were represented as mean ± s.d. of three individual experiments. * p
Figure Legend Snippet: NPM1-mA-mediated INPP4B upregulation promotes cell proliferation in OCI-AML3 cells. The NPM1-mA-silenced OCI-AML3 cells were subjected to ( a ) CCK8 assays and ( b ) colony forming assays. c The NPM1-mA-silenced OCI-AML3 cells were transfected with the pEAK-Flag/INPP4B plasmids, western blotting analysis of INPP4B and NPM1-mA. Proteins were quantified using image software and normalized against β-actin. d CCK-8 assay analysis of cell proliferation in NPM1-mA-silenced OCI-AML3 cells, followed by Flag-INPP4B introduction. Data were represented as mean ± s.d. of three individual experiments. * p

Techniques Used: Transfection, Western Blot, Software, CCK-8 Assay

High expression of INPP4B is associated with poor survival outcome in NPM1-mutated leukemia. Kaplan-Meier survival data of 153 AML patients were used to analysis ( a ) OS and ( b ) EFS curves according to INPP4B levels. c Heatmap of 38 primary AML samples with NPM1 mutation from TCGA dataset in which INPP4B expression was aligned with patient event (Survival or Death). Kaplan-Meier survival data of patients with NPM1-mutated AML were used to analysis ( d ) OS and ( e ) EFS curves according to INPP4B levels. f Schematic diagram describing the functional significance of INPP4B in the NPM1-mutated leukemia cells. INPP4B promotes leukemia cell survival in a SGK3-dependent and AKT-independent manner. The expression of INPP4B partially upregulated by NPM1-mA is due to ERK/Ets-1 signaling
Figure Legend Snippet: High expression of INPP4B is associated with poor survival outcome in NPM1-mutated leukemia. Kaplan-Meier survival data of 153 AML patients were used to analysis ( a ) OS and ( b ) EFS curves according to INPP4B levels. c Heatmap of 38 primary AML samples with NPM1 mutation from TCGA dataset in which INPP4B expression was aligned with patient event (Survival or Death). Kaplan-Meier survival data of patients with NPM1-mutated AML were used to analysis ( d ) OS and ( e ) EFS curves according to INPP4B levels. f Schematic diagram describing the functional significance of INPP4B in the NPM1-mutated leukemia cells. INPP4B promotes leukemia cell survival in a SGK3-dependent and AKT-independent manner. The expression of INPP4B partially upregulated by NPM1-mA is due to ERK/Ets-1 signaling

Techniques Used: Expressing, Mutagenesis, Functional Assay

High expression levels of INPP4B in leukemia cells with the NPM1 mutation. a RNA-seq mRNA expression data from the TCGA database were used to compare INPP4B expression between AML patients with ( n = 41) and without NPM1 mutation ( n = 130). * p
Figure Legend Snippet: High expression levels of INPP4B in leukemia cells with the NPM1 mutation. a RNA-seq mRNA expression data from the TCGA database were used to compare INPP4B expression between AML patients with ( n = 41) and without NPM1 mutation ( n = 130). * p

Techniques Used: Expressing, Mutagenesis, RNA Sequencing Assay

2) Product Images from "INPP4B promotes cell survival via SGK3 activation in NPM1-mutated leukemia"

Article Title: INPP4B promotes cell survival via SGK3 activation in NPM1-mutated leukemia

Journal: Journal of Experimental & Clinical Cancer Research : CR

doi: 10.1186/s13046-018-0675-9

INPP4B is upregulated by NPM1-mA in leukemia cells via ERK/Ets-1 signaling. ( a ) qRT-PCR analysis of INPP4B mRNA expression, ( b ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. ( c ) qRT-PCR analysis of INPP4B mRNA expression, ( d ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the THP-1 and K562 cells transduced with the plasmids expressing NPM1-wt or NPM1-mA. ( e ) qRT-PCR analysis of INPP4B mRNA expression, ( f ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3, p-Ets-1 and Ets-1 from the OCI-AML3 cells transfected with the control siRNA or siEts-1. ( g ) Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 and SGK3, ( h ) qRT-PCR analysis of INPP4B mRNA expression from the OCI-AML3 cells treated with different concentration of PD98059 (0, 10, 20 and 40 μM). i Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. Proteins were quantified using image software and normalized against β-actin. Data were represented as mean ± s.d. of three individual experiments. * p
Figure Legend Snippet: INPP4B is upregulated by NPM1-mA in leukemia cells via ERK/Ets-1 signaling. ( a ) qRT-PCR analysis of INPP4B mRNA expression, ( b ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. ( c ) qRT-PCR analysis of INPP4B mRNA expression, ( d ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the THP-1 and K562 cells transduced with the plasmids expressing NPM1-wt or NPM1-mA. ( e ) qRT-PCR analysis of INPP4B mRNA expression, ( f ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3, p-Ets-1 and Ets-1 from the OCI-AML3 cells transfected with the control siRNA or siEts-1. ( g ) Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 and SGK3, ( h ) qRT-PCR analysis of INPP4B mRNA expression from the OCI-AML3 cells treated with different concentration of PD98059 (0, 10, 20 and 40 μM). i Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. Proteins were quantified using image software and normalized against β-actin. Data were represented as mean ± s.d. of three individual experiments. * p

Techniques Used: Quantitative RT-PCR, Expressing, Western Blot, Transduction, Transfection, Concentration Assay, Software

NPM1-mA-mediated INPP4B upregulation promotes cell proliferation in OCI-AML3 cells. The NPM1-mA-silenced OCI-AML3 cells were subjected to ( a ) CCK8 assays and ( b ) colony forming assays. c The NPM1-mA-silenced OCI-AML3 cells were transfected with the pEAK-Flag/INPP4B plasmids, western blotting analysis of INPP4B and NPM1-mA. Proteins were quantified using image software and normalized against β-actin. d CCK-8 assay analysis of cell proliferation in NPM1-mA-silenced OCI-AML3 cells, followed by Flag-INPP4B introduction. Data were represented as mean ± s.d. of three individual experiments. * p
Figure Legend Snippet: NPM1-mA-mediated INPP4B upregulation promotes cell proliferation in OCI-AML3 cells. The NPM1-mA-silenced OCI-AML3 cells were subjected to ( a ) CCK8 assays and ( b ) colony forming assays. c The NPM1-mA-silenced OCI-AML3 cells were transfected with the pEAK-Flag/INPP4B plasmids, western blotting analysis of INPP4B and NPM1-mA. Proteins were quantified using image software and normalized against β-actin. d CCK-8 assay analysis of cell proliferation in NPM1-mA-silenced OCI-AML3 cells, followed by Flag-INPP4B introduction. Data were represented as mean ± s.d. of three individual experiments. * p

Techniques Used: Transfection, Western Blot, Software, CCK-8 Assay

High expression of INPP4B is associated with poor survival outcome in NPM1-mutated leukemia. Kaplan-Meier survival data of 153 AML patients were used to analysis ( a ) OS and ( b ) EFS curves according to INPP4B levels. c Heatmap of 38 primary AML samples with NPM1 mutation from TCGA dataset in which INPP4B expression was aligned with patient event (Survival or Death). Kaplan-Meier survival data of patients with NPM1-mutated AML were used to analysis ( d ) OS and ( e ) EFS curves according to INPP4B levels. f Schematic diagram describing the functional significance of INPP4B in the NPM1-mutated leukemia cells. INPP4B promotes leukemia cell survival in a SGK3-dependent and AKT-independent manner. The expression of INPP4B partially upregulated by NPM1-mA is due to ERK/Ets-1 signaling
Figure Legend Snippet: High expression of INPP4B is associated with poor survival outcome in NPM1-mutated leukemia. Kaplan-Meier survival data of 153 AML patients were used to analysis ( a ) OS and ( b ) EFS curves according to INPP4B levels. c Heatmap of 38 primary AML samples with NPM1 mutation from TCGA dataset in which INPP4B expression was aligned with patient event (Survival or Death). Kaplan-Meier survival data of patients with NPM1-mutated AML were used to analysis ( d ) OS and ( e ) EFS curves according to INPP4B levels. f Schematic diagram describing the functional significance of INPP4B in the NPM1-mutated leukemia cells. INPP4B promotes leukemia cell survival in a SGK3-dependent and AKT-independent manner. The expression of INPP4B partially upregulated by NPM1-mA is due to ERK/Ets-1 signaling

Techniques Used: Expressing, Mutagenesis, Functional Assay

High expression levels of INPP4B in leukemia cells with the NPM1 mutation. a RNA-seq mRNA expression data from the TCGA database were used to compare INPP4B expression between AML patients with ( n = 41) and without NPM1 mutation ( n = 130). * p
Figure Legend Snippet: High expression levels of INPP4B in leukemia cells with the NPM1 mutation. a RNA-seq mRNA expression data from the TCGA database were used to compare INPP4B expression between AML patients with ( n = 41) and without NPM1 mutation ( n = 130). * p

Techniques Used: Expressing, Mutagenesis, RNA Sequencing Assay

3) Product Images from "INPP4B promotes cell survival via SGK3 activation in NPM1-mutated leukemia"

Article Title: INPP4B promotes cell survival via SGK3 activation in NPM1-mutated leukemia

Journal: Journal of Experimental & Clinical Cancer Research : CR

doi: 10.1186/s13046-018-0675-9

INPP4B is upregulated by NPM1-mA in leukemia cells via ERK/Ets-1 signaling. ( a ) qRT-PCR analysis of INPP4B mRNA expression, ( b ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. ( c ) qRT-PCR analysis of INPP4B mRNA expression, ( d ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the THP-1 and K562 cells transduced with the plasmids expressing NPM1-wt or NPM1-mA. ( e ) qRT-PCR analysis of INPP4B mRNA expression, ( f ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3, p-Ets-1 and Ets-1 from the OCI-AML3 cells transfected with the control siRNA or siEts-1. ( g ) Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 and SGK3, ( h ) qRT-PCR analysis of INPP4B mRNA expression from the OCI-AML3 cells treated with different concentration of PD98059 (0, 10, 20 and 40 μM). i Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. Proteins were quantified using image software and normalized against β-actin. Data were represented as mean ± s.d. of three individual experiments. * p
Figure Legend Snippet: INPP4B is upregulated by NPM1-mA in leukemia cells via ERK/Ets-1 signaling. ( a ) qRT-PCR analysis of INPP4B mRNA expression, ( b ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. ( c ) qRT-PCR analysis of INPP4B mRNA expression, ( d ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the THP-1 and K562 cells transduced with the plasmids expressing NPM1-wt or NPM1-mA. ( e ) qRT-PCR analysis of INPP4B mRNA expression, ( f ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3, p-Ets-1 and Ets-1 from the OCI-AML3 cells transfected with the control siRNA or siEts-1. ( g ) Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 and SGK3, ( h ) qRT-PCR analysis of INPP4B mRNA expression from the OCI-AML3 cells treated with different concentration of PD98059 (0, 10, 20 and 40 μM). i Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. Proteins were quantified using image software and normalized against β-actin. Data were represented as mean ± s.d. of three individual experiments. * p

Techniques Used: Quantitative RT-PCR, Expressing, Western Blot, Transduction, Transfection, Concentration Assay, Software

NPM1-mA-mediated INPP4B upregulation promotes cell proliferation in OCI-AML3 cells. The NPM1-mA-silenced OCI-AML3 cells were subjected to ( a ) CCK8 assays and ( b ) colony forming assays. c The NPM1-mA-silenced OCI-AML3 cells were transfected with the pEAK-Flag/INPP4B plasmids, western blotting analysis of INPP4B and NPM1-mA. Proteins were quantified using image software and normalized against β-actin. d CCK-8 assay analysis of cell proliferation in NPM1-mA-silenced OCI-AML3 cells, followed by Flag-INPP4B introduction. Data were represented as mean ± s.d. of three individual experiments. * p
Figure Legend Snippet: NPM1-mA-mediated INPP4B upregulation promotes cell proliferation in OCI-AML3 cells. The NPM1-mA-silenced OCI-AML3 cells were subjected to ( a ) CCK8 assays and ( b ) colony forming assays. c The NPM1-mA-silenced OCI-AML3 cells were transfected with the pEAK-Flag/INPP4B plasmids, western blotting analysis of INPP4B and NPM1-mA. Proteins were quantified using image software and normalized against β-actin. d CCK-8 assay analysis of cell proliferation in NPM1-mA-silenced OCI-AML3 cells, followed by Flag-INPP4B introduction. Data were represented as mean ± s.d. of three individual experiments. * p

Techniques Used: Transfection, Western Blot, Software, CCK-8 Assay

High expression of INPP4B is associated with poor survival outcome in NPM1-mutated leukemia. Kaplan-Meier survival data of 153 AML patients were used to analysis ( a ) OS and ( b ) EFS curves according to INPP4B levels. c Heatmap of 38 primary AML samples with NPM1 mutation from TCGA dataset in which INPP4B expression was aligned with patient event (Survival or Death). Kaplan-Meier survival data of patients with NPM1-mutated AML were used to analysis ( d ) OS and ( e ) EFS curves according to INPP4B levels. f Schematic diagram describing the functional significance of INPP4B in the NPM1-mutated leukemia cells. INPP4B promotes leukemia cell survival in a SGK3-dependent and AKT-independent manner. The expression of INPP4B partially upregulated by NPM1-mA is due to ERK/Ets-1 signaling
Figure Legend Snippet: High expression of INPP4B is associated with poor survival outcome in NPM1-mutated leukemia. Kaplan-Meier survival data of 153 AML patients were used to analysis ( a ) OS and ( b ) EFS curves according to INPP4B levels. c Heatmap of 38 primary AML samples with NPM1 mutation from TCGA dataset in which INPP4B expression was aligned with patient event (Survival or Death). Kaplan-Meier survival data of patients with NPM1-mutated AML were used to analysis ( d ) OS and ( e ) EFS curves according to INPP4B levels. f Schematic diagram describing the functional significance of INPP4B in the NPM1-mutated leukemia cells. INPP4B promotes leukemia cell survival in a SGK3-dependent and AKT-independent manner. The expression of INPP4B partially upregulated by NPM1-mA is due to ERK/Ets-1 signaling

Techniques Used: Expressing, Mutagenesis, Functional Assay

High expression levels of INPP4B in leukemia cells with the NPM1 mutation. a RNA-seq mRNA expression data from the TCGA database were used to compare INPP4B expression between AML patients with ( n = 41) and without NPM1 mutation ( n = 130). * p
Figure Legend Snippet: High expression levels of INPP4B in leukemia cells with the NPM1 mutation. a RNA-seq mRNA expression data from the TCGA database were used to compare INPP4B expression between AML patients with ( n = 41) and without NPM1 mutation ( n = 130). * p

Techniques Used: Expressing, Mutagenesis, RNA Sequencing Assay

4) Product Images from "INPP4B promotes cell survival via SGK3 activation in NPM1-mutated leukemia"

Article Title: INPP4B promotes cell survival via SGK3 activation in NPM1-mutated leukemia

Journal: Journal of Experimental & Clinical Cancer Research : CR

doi: 10.1186/s13046-018-0675-9

INPP4B is upregulated by NPM1-mA in leukemia cells via ERK/Ets-1 signaling. ( a ) qRT-PCR analysis of INPP4B mRNA expression, ( b ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. ( c ) qRT-PCR analysis of INPP4B mRNA expression, ( d ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the THP-1 and K562 cells transduced with the plasmids expressing NPM1-wt or NPM1-mA. ( e ) qRT-PCR analysis of INPP4B mRNA expression, ( f ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3, p-Ets-1 and Ets-1 from the OCI-AML3 cells transfected with the control siRNA or siEts-1. ( g ) Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 and SGK3, ( h ) qRT-PCR analysis of INPP4B mRNA expression from the OCI-AML3 cells treated with different concentration of PD98059 (0, 10, 20 and 40 μM). i Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. Proteins were quantified using image software and normalized against β-actin. Data were represented as mean ± s.d. of three individual experiments. * p
Figure Legend Snippet: INPP4B is upregulated by NPM1-mA in leukemia cells via ERK/Ets-1 signaling. ( a ) qRT-PCR analysis of INPP4B mRNA expression, ( b ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. ( c ) qRT-PCR analysis of INPP4B mRNA expression, ( d ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the THP-1 and K562 cells transduced with the plasmids expressing NPM1-wt or NPM1-mA. ( e ) qRT-PCR analysis of INPP4B mRNA expression, ( f ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3, p-Ets-1 and Ets-1 from the OCI-AML3 cells transfected with the control siRNA or siEts-1. ( g ) Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 and SGK3, ( h ) qRT-PCR analysis of INPP4B mRNA expression from the OCI-AML3 cells treated with different concentration of PD98059 (0, 10, 20 and 40 μM). i Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. Proteins were quantified using image software and normalized against β-actin. Data were represented as mean ± s.d. of three individual experiments. * p

Techniques Used: Quantitative RT-PCR, Expressing, Western Blot, Transduction, Transfection, Concentration Assay, Software

NPM1-mA-mediated INPP4B upregulation promotes cell proliferation in OCI-AML3 cells. The NPM1-mA-silenced OCI-AML3 cells were subjected to ( a ) CCK8 assays and ( b ) colony forming assays. c The NPM1-mA-silenced OCI-AML3 cells were transfected with the pEAK-Flag/INPP4B plasmids, western blotting analysis of INPP4B and NPM1-mA. Proteins were quantified using image software and normalized against β-actin. d CCK-8 assay analysis of cell proliferation in NPM1-mA-silenced OCI-AML3 cells, followed by Flag-INPP4B introduction. Data were represented as mean ± s.d. of three individual experiments. * p
Figure Legend Snippet: NPM1-mA-mediated INPP4B upregulation promotes cell proliferation in OCI-AML3 cells. The NPM1-mA-silenced OCI-AML3 cells were subjected to ( a ) CCK8 assays and ( b ) colony forming assays. c The NPM1-mA-silenced OCI-AML3 cells were transfected with the pEAK-Flag/INPP4B plasmids, western blotting analysis of INPP4B and NPM1-mA. Proteins were quantified using image software and normalized against β-actin. d CCK-8 assay analysis of cell proliferation in NPM1-mA-silenced OCI-AML3 cells, followed by Flag-INPP4B introduction. Data were represented as mean ± s.d. of three individual experiments. * p

Techniques Used: Transfection, Western Blot, Software, CCK-8 Assay

High expression of INPP4B is associated with poor survival outcome in NPM1-mutated leukemia. Kaplan-Meier survival data of 153 AML patients were used to analysis ( a ) OS and ( b ) EFS curves according to INPP4B levels. c Heatmap of 38 primary AML samples with NPM1 mutation from TCGA dataset in which INPP4B expression was aligned with patient event (Survival or Death). Kaplan-Meier survival data of patients with NPM1-mutated AML were used to analysis ( d ) OS and ( e ) EFS curves according to INPP4B levels. f Schematic diagram describing the functional significance of INPP4B in the NPM1-mutated leukemia cells. INPP4B promotes leukemia cell survival in a SGK3-dependent and AKT-independent manner. The expression of INPP4B partially upregulated by NPM1-mA is due to ERK/Ets-1 signaling
Figure Legend Snippet: High expression of INPP4B is associated with poor survival outcome in NPM1-mutated leukemia. Kaplan-Meier survival data of 153 AML patients were used to analysis ( a ) OS and ( b ) EFS curves according to INPP4B levels. c Heatmap of 38 primary AML samples with NPM1 mutation from TCGA dataset in which INPP4B expression was aligned with patient event (Survival or Death). Kaplan-Meier survival data of patients with NPM1-mutated AML were used to analysis ( d ) OS and ( e ) EFS curves according to INPP4B levels. f Schematic diagram describing the functional significance of INPP4B in the NPM1-mutated leukemia cells. INPP4B promotes leukemia cell survival in a SGK3-dependent and AKT-independent manner. The expression of INPP4B partially upregulated by NPM1-mA is due to ERK/Ets-1 signaling

Techniques Used: Expressing, Mutagenesis, Functional Assay

High expression levels of INPP4B in leukemia cells with the NPM1 mutation. a RNA-seq mRNA expression data from the TCGA database were used to compare INPP4B expression between AML patients with ( n = 41) and without NPM1 mutation ( n = 130). * p
Figure Legend Snippet: High expression levels of INPP4B in leukemia cells with the NPM1 mutation. a RNA-seq mRNA expression data from the TCGA database were used to compare INPP4B expression between AML patients with ( n = 41) and without NPM1 mutation ( n = 130). * p

Techniques Used: Expressing, Mutagenesis, RNA Sequencing Assay

5) Product Images from "INPP4B promotes cell survival via SGK3 activation in NPM1-mutated leukemia"

Article Title: INPP4B promotes cell survival via SGK3 activation in NPM1-mutated leukemia

Journal: Journal of Experimental & Clinical Cancer Research : CR

doi: 10.1186/s13046-018-0675-9

INPP4B is upregulated by NPM1-mA in leukemia cells via ERK/Ets-1 signaling. ( a ) qRT-PCR analysis of INPP4B mRNA expression, ( b ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. ( c ) qRT-PCR analysis of INPP4B mRNA expression, ( d ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the THP-1 and K562 cells transduced with the plasmids expressing NPM1-wt or NPM1-mA. ( e ) qRT-PCR analysis of INPP4B mRNA expression, ( f ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3, p-Ets-1 and Ets-1 from the OCI-AML3 cells transfected with the control siRNA or siEts-1. ( g ) Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 and SGK3, ( h ) qRT-PCR analysis of INPP4B mRNA expression from the OCI-AML3 cells treated with different concentration of PD98059 (0, 10, 20 and 40 μM). i Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. Proteins were quantified using image software and normalized against β-actin. Data were represented as mean ± s.d. of three individual experiments. * p
Figure Legend Snippet: INPP4B is upregulated by NPM1-mA in leukemia cells via ERK/Ets-1 signaling. ( a ) qRT-PCR analysis of INPP4B mRNA expression, ( b ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. ( c ) qRT-PCR analysis of INPP4B mRNA expression, ( d ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the THP-1 and K562 cells transduced with the plasmids expressing NPM1-wt or NPM1-mA. ( e ) qRT-PCR analysis of INPP4B mRNA expression, ( f ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3, p-Ets-1 and Ets-1 from the OCI-AML3 cells transfected with the control siRNA or siEts-1. ( g ) Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 and SGK3, ( h ) qRT-PCR analysis of INPP4B mRNA expression from the OCI-AML3 cells treated with different concentration of PD98059 (0, 10, 20 and 40 μM). i Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. Proteins were quantified using image software and normalized against β-actin. Data were represented as mean ± s.d. of three individual experiments. * p

Techniques Used: Quantitative RT-PCR, Expressing, Western Blot, Transduction, Transfection, Concentration Assay, Software

NPM1-mA-mediated INPP4B upregulation promotes cell proliferation in OCI-AML3 cells. The NPM1-mA-silenced OCI-AML3 cells were subjected to ( a ) CCK8 assays and ( b ) colony forming assays. c The NPM1-mA-silenced OCI-AML3 cells were transfected with the pEAK-Flag/INPP4B plasmids, western blotting analysis of INPP4B and NPM1-mA. Proteins were quantified using image software and normalized against β-actin. d CCK-8 assay analysis of cell proliferation in NPM1-mA-silenced OCI-AML3 cells, followed by Flag-INPP4B introduction. Data were represented as mean ± s.d. of three individual experiments. * p
Figure Legend Snippet: NPM1-mA-mediated INPP4B upregulation promotes cell proliferation in OCI-AML3 cells. The NPM1-mA-silenced OCI-AML3 cells were subjected to ( a ) CCK8 assays and ( b ) colony forming assays. c The NPM1-mA-silenced OCI-AML3 cells were transfected with the pEAK-Flag/INPP4B plasmids, western blotting analysis of INPP4B and NPM1-mA. Proteins were quantified using image software and normalized against β-actin. d CCK-8 assay analysis of cell proliferation in NPM1-mA-silenced OCI-AML3 cells, followed by Flag-INPP4B introduction. Data were represented as mean ± s.d. of three individual experiments. * p

Techniques Used: Transfection, Western Blot, Software, CCK-8 Assay

High expression of INPP4B is associated with poor survival outcome in NPM1-mutated leukemia. Kaplan-Meier survival data of 153 AML patients were used to analysis ( a ) OS and ( b ) EFS curves according to INPP4B levels. c Heatmap of 38 primary AML samples with NPM1 mutation from TCGA dataset in which INPP4B expression was aligned with patient event (Survival or Death). Kaplan-Meier survival data of patients with NPM1-mutated AML were used to analysis ( d ) OS and ( e ) EFS curves according to INPP4B levels. f Schematic diagram describing the functional significance of INPP4B in the NPM1-mutated leukemia cells. INPP4B promotes leukemia cell survival in a SGK3-dependent and AKT-independent manner. The expression of INPP4B partially upregulated by NPM1-mA is due to ERK/Ets-1 signaling
Figure Legend Snippet: High expression of INPP4B is associated with poor survival outcome in NPM1-mutated leukemia. Kaplan-Meier survival data of 153 AML patients were used to analysis ( a ) OS and ( b ) EFS curves according to INPP4B levels. c Heatmap of 38 primary AML samples with NPM1 mutation from TCGA dataset in which INPP4B expression was aligned with patient event (Survival or Death). Kaplan-Meier survival data of patients with NPM1-mutated AML were used to analysis ( d ) OS and ( e ) EFS curves according to INPP4B levels. f Schematic diagram describing the functional significance of INPP4B in the NPM1-mutated leukemia cells. INPP4B promotes leukemia cell survival in a SGK3-dependent and AKT-independent manner. The expression of INPP4B partially upregulated by NPM1-mA is due to ERK/Ets-1 signaling

Techniques Used: Expressing, Mutagenesis, Functional Assay

High expression levels of INPP4B in leukemia cells with the NPM1 mutation. a RNA-seq mRNA expression data from the TCGA database were used to compare INPP4B expression between AML patients with ( n = 41) and without NPM1 mutation ( n = 130). * p
Figure Legend Snippet: High expression levels of INPP4B in leukemia cells with the NPM1 mutation. a RNA-seq mRNA expression data from the TCGA database were used to compare INPP4B expression between AML patients with ( n = 41) and without NPM1 mutation ( n = 130). * p

Techniques Used: Expressing, Mutagenesis, RNA Sequencing Assay

6) Product Images from "INPP4B promotes cell survival via SGK3 activation in NPM1-mutated leukemia"

Article Title: INPP4B promotes cell survival via SGK3 activation in NPM1-mutated leukemia

Journal: Journal of Experimental & Clinical Cancer Research : CR

doi: 10.1186/s13046-018-0675-9

INPP4B is upregulated by NPM1-mA in leukemia cells via ERK/Ets-1 signaling. ( a ) qRT-PCR analysis of INPP4B mRNA expression, ( b ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. ( c ) qRT-PCR analysis of INPP4B mRNA expression, ( d ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the THP-1 and K562 cells transduced with the plasmids expressing NPM1-wt or NPM1-mA. ( e ) qRT-PCR analysis of INPP4B mRNA expression, ( f ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3, p-Ets-1 and Ets-1 from the OCI-AML3 cells transfected with the control siRNA or siEts-1. ( g ) Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 and SGK3, ( h ) qRT-PCR analysis of INPP4B mRNA expression from the OCI-AML3 cells treated with different concentration of PD98059 (0, 10, 20 and 40 μM). i Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. Proteins were quantified using image software and normalized against β-actin. Data were represented as mean ± s.d. of three individual experiments. * p
Figure Legend Snippet: INPP4B is upregulated by NPM1-mA in leukemia cells via ERK/Ets-1 signaling. ( a ) qRT-PCR analysis of INPP4B mRNA expression, ( b ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. ( c ) qRT-PCR analysis of INPP4B mRNA expression, ( d ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the THP-1 and K562 cells transduced with the plasmids expressing NPM1-wt or NPM1-mA. ( e ) qRT-PCR analysis of INPP4B mRNA expression, ( f ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3, p-Ets-1 and Ets-1 from the OCI-AML3 cells transfected with the control siRNA or siEts-1. ( g ) Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 and SGK3, ( h ) qRT-PCR analysis of INPP4B mRNA expression from the OCI-AML3 cells treated with different concentration of PD98059 (0, 10, 20 and 40 μM). i Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. Proteins were quantified using image software and normalized against β-actin. Data were represented as mean ± s.d. of three individual experiments. * p

Techniques Used: Quantitative RT-PCR, Expressing, Western Blot, Transduction, Transfection, Concentration Assay, Software

NPM1-mA-mediated INPP4B upregulation promotes cell proliferation in OCI-AML3 cells. The NPM1-mA-silenced OCI-AML3 cells were subjected to ( a ) CCK8 assays and ( b ) colony forming assays. c The NPM1-mA-silenced OCI-AML3 cells were transfected with the pEAK-Flag/INPP4B plasmids, western blotting analysis of INPP4B and NPM1-mA. Proteins were quantified using image software and normalized against β-actin. d CCK-8 assay analysis of cell proliferation in NPM1-mA-silenced OCI-AML3 cells, followed by Flag-INPP4B introduction. Data were represented as mean ± s.d. of three individual experiments. * p
Figure Legend Snippet: NPM1-mA-mediated INPP4B upregulation promotes cell proliferation in OCI-AML3 cells. The NPM1-mA-silenced OCI-AML3 cells were subjected to ( a ) CCK8 assays and ( b ) colony forming assays. c The NPM1-mA-silenced OCI-AML3 cells were transfected with the pEAK-Flag/INPP4B plasmids, western blotting analysis of INPP4B and NPM1-mA. Proteins were quantified using image software and normalized against β-actin. d CCK-8 assay analysis of cell proliferation in NPM1-mA-silenced OCI-AML3 cells, followed by Flag-INPP4B introduction. Data were represented as mean ± s.d. of three individual experiments. * p

Techniques Used: Transfection, Western Blot, Software, CCK-8 Assay

High expression of INPP4B is associated with poor survival outcome in NPM1-mutated leukemia. Kaplan-Meier survival data of 153 AML patients were used to analysis ( a ) OS and ( b ) EFS curves according to INPP4B levels. c Heatmap of 38 primary AML samples with NPM1 mutation from TCGA dataset in which INPP4B expression was aligned with patient event (Survival or Death). Kaplan-Meier survival data of patients with NPM1-mutated AML were used to analysis ( d ) OS and ( e ) EFS curves according to INPP4B levels. f Schematic diagram describing the functional significance of INPP4B in the NPM1-mutated leukemia cells. INPP4B promotes leukemia cell survival in a SGK3-dependent and AKT-independent manner. The expression of INPP4B partially upregulated by NPM1-mA is due to ERK/Ets-1 signaling
Figure Legend Snippet: High expression of INPP4B is associated with poor survival outcome in NPM1-mutated leukemia. Kaplan-Meier survival data of 153 AML patients were used to analysis ( a ) OS and ( b ) EFS curves according to INPP4B levels. c Heatmap of 38 primary AML samples with NPM1 mutation from TCGA dataset in which INPP4B expression was aligned with patient event (Survival or Death). Kaplan-Meier survival data of patients with NPM1-mutated AML were used to analysis ( d ) OS and ( e ) EFS curves according to INPP4B levels. f Schematic diagram describing the functional significance of INPP4B in the NPM1-mutated leukemia cells. INPP4B promotes leukemia cell survival in a SGK3-dependent and AKT-independent manner. The expression of INPP4B partially upregulated by NPM1-mA is due to ERK/Ets-1 signaling

Techniques Used: Expressing, Mutagenesis, Functional Assay

High expression levels of INPP4B in leukemia cells with the NPM1 mutation. a RNA-seq mRNA expression data from the TCGA database were used to compare INPP4B expression between AML patients with ( n = 41) and without NPM1 mutation ( n = 130). * p
Figure Legend Snippet: High expression levels of INPP4B in leukemia cells with the NPM1 mutation. a RNA-seq mRNA expression data from the TCGA database were used to compare INPP4B expression between AML patients with ( n = 41) and without NPM1 mutation ( n = 130). * p

Techniques Used: Expressing, Mutagenesis, RNA Sequencing Assay

7) Product Images from "INPP4B promotes cell survival via SGK3 activation in NPM1-mutated leukemia"

Article Title: INPP4B promotes cell survival via SGK3 activation in NPM1-mutated leukemia

Journal: Journal of Experimental & Clinical Cancer Research : CR

doi: 10.1186/s13046-018-0675-9

INPP4B is upregulated by NPM1-mA in leukemia cells via ERK/Ets-1 signaling. ( a ) qRT-PCR analysis of INPP4B mRNA expression, ( b ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. ( c ) qRT-PCR analysis of INPP4B mRNA expression, ( d ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the THP-1 and K562 cells transduced with the plasmids expressing NPM1-wt or NPM1-mA. ( e ) qRT-PCR analysis of INPP4B mRNA expression, ( f ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3, p-Ets-1 and Ets-1 from the OCI-AML3 cells transfected with the control siRNA or siEts-1. ( g ) Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 and SGK3, ( h ) qRT-PCR analysis of INPP4B mRNA expression from the OCI-AML3 cells treated with different concentration of PD98059 (0, 10, 20 and 40 μM). i Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. Proteins were quantified using image software and normalized against β-actin. Data were represented as mean ± s.d. of three individual experiments. * p
Figure Legend Snippet: INPP4B is upregulated by NPM1-mA in leukemia cells via ERK/Ets-1 signaling. ( a ) qRT-PCR analysis of INPP4B mRNA expression, ( b ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. ( c ) qRT-PCR analysis of INPP4B mRNA expression, ( d ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the THP-1 and K562 cells transduced with the plasmids expressing NPM1-wt or NPM1-mA. ( e ) qRT-PCR analysis of INPP4B mRNA expression, ( f ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3, p-Ets-1 and Ets-1 from the OCI-AML3 cells transfected with the control siRNA or siEts-1. ( g ) Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 and SGK3, ( h ) qRT-PCR analysis of INPP4B mRNA expression from the OCI-AML3 cells treated with different concentration of PD98059 (0, 10, 20 and 40 μM). i Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. Proteins were quantified using image software and normalized against β-actin. Data were represented as mean ± s.d. of three individual experiments. * p

Techniques Used: Quantitative RT-PCR, Expressing, Western Blot, Transduction, Transfection, Concentration Assay, Software

NPM1-mA-mediated INPP4B upregulation promotes cell proliferation in OCI-AML3 cells. The NPM1-mA-silenced OCI-AML3 cells were subjected to ( a ) CCK8 assays and ( b ) colony forming assays. c The NPM1-mA-silenced OCI-AML3 cells were transfected with the pEAK-Flag/INPP4B plasmids, western blotting analysis of INPP4B and NPM1-mA. Proteins were quantified using image software and normalized against β-actin. d CCK-8 assay analysis of cell proliferation in NPM1-mA-silenced OCI-AML3 cells, followed by Flag-INPP4B introduction. Data were represented as mean ± s.d. of three individual experiments. * p
Figure Legend Snippet: NPM1-mA-mediated INPP4B upregulation promotes cell proliferation in OCI-AML3 cells. The NPM1-mA-silenced OCI-AML3 cells were subjected to ( a ) CCK8 assays and ( b ) colony forming assays. c The NPM1-mA-silenced OCI-AML3 cells were transfected with the pEAK-Flag/INPP4B plasmids, western blotting analysis of INPP4B and NPM1-mA. Proteins were quantified using image software and normalized against β-actin. d CCK-8 assay analysis of cell proliferation in NPM1-mA-silenced OCI-AML3 cells, followed by Flag-INPP4B introduction. Data were represented as mean ± s.d. of three individual experiments. * p

Techniques Used: Transfection, Western Blot, Software, CCK-8 Assay

High expression of INPP4B is associated with poor survival outcome in NPM1-mutated leukemia. Kaplan-Meier survival data of 153 AML patients were used to analysis ( a ) OS and ( b ) EFS curves according to INPP4B levels. c Heatmap of 38 primary AML samples with NPM1 mutation from TCGA dataset in which INPP4B expression was aligned with patient event (Survival or Death). Kaplan-Meier survival data of patients with NPM1-mutated AML were used to analysis ( d ) OS and ( e ) EFS curves according to INPP4B levels. f Schematic diagram describing the functional significance of INPP4B in the NPM1-mutated leukemia cells. INPP4B promotes leukemia cell survival in a SGK3-dependent and AKT-independent manner. The expression of INPP4B partially upregulated by NPM1-mA is due to ERK/Ets-1 signaling
Figure Legend Snippet: High expression of INPP4B is associated with poor survival outcome in NPM1-mutated leukemia. Kaplan-Meier survival data of 153 AML patients were used to analysis ( a ) OS and ( b ) EFS curves according to INPP4B levels. c Heatmap of 38 primary AML samples with NPM1 mutation from TCGA dataset in which INPP4B expression was aligned with patient event (Survival or Death). Kaplan-Meier survival data of patients with NPM1-mutated AML were used to analysis ( d ) OS and ( e ) EFS curves according to INPP4B levels. f Schematic diagram describing the functional significance of INPP4B in the NPM1-mutated leukemia cells. INPP4B promotes leukemia cell survival in a SGK3-dependent and AKT-independent manner. The expression of INPP4B partially upregulated by NPM1-mA is due to ERK/Ets-1 signaling

Techniques Used: Expressing, Mutagenesis, Functional Assay

High expression levels of INPP4B in leukemia cells with the NPM1 mutation. a RNA-seq mRNA expression data from the TCGA database were used to compare INPP4B expression between AML patients with ( n = 41) and without NPM1 mutation ( n = 130). * p
Figure Legend Snippet: High expression levels of INPP4B in leukemia cells with the NPM1 mutation. a RNA-seq mRNA expression data from the TCGA database were used to compare INPP4B expression between AML patients with ( n = 41) and without NPM1 mutation ( n = 130). * p

Techniques Used: Expressing, Mutagenesis, RNA Sequencing Assay

8) Product Images from "INPP4B promotes cell survival via SGK3 activation in NPM1-mutated leukemia"

Article Title: INPP4B promotes cell survival via SGK3 activation in NPM1-mutated leukemia

Journal: Journal of Experimental & Clinical Cancer Research : CR

doi: 10.1186/s13046-018-0675-9

INPP4B is upregulated by NPM1-mA in leukemia cells via ERK/Ets-1 signaling. ( a ) qRT-PCR analysis of INPP4B mRNA expression, ( b ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. ( c ) qRT-PCR analysis of INPP4B mRNA expression, ( d ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the THP-1 and K562 cells transduced with the plasmids expressing NPM1-wt or NPM1-mA. ( e ) qRT-PCR analysis of INPP4B mRNA expression, ( f ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3, p-Ets-1 and Ets-1 from the OCI-AML3 cells transfected with the control siRNA or siEts-1. ( g ) Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 and SGK3, ( h ) qRT-PCR analysis of INPP4B mRNA expression from the OCI-AML3 cells treated with different concentration of PD98059 (0, 10, 20 and 40 μM). i Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. Proteins were quantified using image software and normalized against β-actin. Data were represented as mean ± s.d. of three individual experiments. * p
Figure Legend Snippet: INPP4B is upregulated by NPM1-mA in leukemia cells via ERK/Ets-1 signaling. ( a ) qRT-PCR analysis of INPP4B mRNA expression, ( b ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. ( c ) qRT-PCR analysis of INPP4B mRNA expression, ( d ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the THP-1 and K562 cells transduced with the plasmids expressing NPM1-wt or NPM1-mA. ( e ) qRT-PCR analysis of INPP4B mRNA expression, ( f ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3, p-Ets-1 and Ets-1 from the OCI-AML3 cells transfected with the control siRNA or siEts-1. ( g ) Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 and SGK3, ( h ) qRT-PCR analysis of INPP4B mRNA expression from the OCI-AML3 cells treated with different concentration of PD98059 (0, 10, 20 and 40 μM). i Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. Proteins were quantified using image software and normalized against β-actin. Data were represented as mean ± s.d. of three individual experiments. * p

Techniques Used: Quantitative RT-PCR, Expressing, Western Blot, Transduction, Transfection, Concentration Assay, Software

NPM1-mA-mediated INPP4B upregulation promotes cell proliferation in OCI-AML3 cells. The NPM1-mA-silenced OCI-AML3 cells were subjected to ( a ) CCK8 assays and ( b ) colony forming assays. c The NPM1-mA-silenced OCI-AML3 cells were transfected with the pEAK-Flag/INPP4B plasmids, western blotting analysis of INPP4B and NPM1-mA. Proteins were quantified using image software and normalized against β-actin. d CCK-8 assay analysis of cell proliferation in NPM1-mA-silenced OCI-AML3 cells, followed by Flag-INPP4B introduction. Data were represented as mean ± s.d. of three individual experiments. * p
Figure Legend Snippet: NPM1-mA-mediated INPP4B upregulation promotes cell proliferation in OCI-AML3 cells. The NPM1-mA-silenced OCI-AML3 cells were subjected to ( a ) CCK8 assays and ( b ) colony forming assays. c The NPM1-mA-silenced OCI-AML3 cells were transfected with the pEAK-Flag/INPP4B plasmids, western blotting analysis of INPP4B and NPM1-mA. Proteins were quantified using image software and normalized against β-actin. d CCK-8 assay analysis of cell proliferation in NPM1-mA-silenced OCI-AML3 cells, followed by Flag-INPP4B introduction. Data were represented as mean ± s.d. of three individual experiments. * p

Techniques Used: Transfection, Western Blot, Software, CCK-8 Assay

High expression of INPP4B is associated with poor survival outcome in NPM1-mutated leukemia. Kaplan-Meier survival data of 153 AML patients were used to analysis ( a ) OS and ( b ) EFS curves according to INPP4B levels. c Heatmap of 38 primary AML samples with NPM1 mutation from TCGA dataset in which INPP4B expression was aligned with patient event (Survival or Death). Kaplan-Meier survival data of patients with NPM1-mutated AML were used to analysis ( d ) OS and ( e ) EFS curves according to INPP4B levels. f Schematic diagram describing the functional significance of INPP4B in the NPM1-mutated leukemia cells. INPP4B promotes leukemia cell survival in a SGK3-dependent and AKT-independent manner. The expression of INPP4B partially upregulated by NPM1-mA is due to ERK/Ets-1 signaling
Figure Legend Snippet: High expression of INPP4B is associated with poor survival outcome in NPM1-mutated leukemia. Kaplan-Meier survival data of 153 AML patients were used to analysis ( a ) OS and ( b ) EFS curves according to INPP4B levels. c Heatmap of 38 primary AML samples with NPM1 mutation from TCGA dataset in which INPP4B expression was aligned with patient event (Survival or Death). Kaplan-Meier survival data of patients with NPM1-mutated AML were used to analysis ( d ) OS and ( e ) EFS curves according to INPP4B levels. f Schematic diagram describing the functional significance of INPP4B in the NPM1-mutated leukemia cells. INPP4B promotes leukemia cell survival in a SGK3-dependent and AKT-independent manner. The expression of INPP4B partially upregulated by NPM1-mA is due to ERK/Ets-1 signaling

Techniques Used: Expressing, Mutagenesis, Functional Assay

High expression levels of INPP4B in leukemia cells with the NPM1 mutation. a RNA-seq mRNA expression data from the TCGA database were used to compare INPP4B expression between AML patients with ( n = 41) and without NPM1 mutation ( n = 130). * p
Figure Legend Snippet: High expression levels of INPP4B in leukemia cells with the NPM1 mutation. a RNA-seq mRNA expression data from the TCGA database were used to compare INPP4B expression between AML patients with ( n = 41) and without NPM1 mutation ( n = 130). * p

Techniques Used: Expressing, Mutagenesis, RNA Sequencing Assay

9) Product Images from "INPP4B promotes cell survival via SGK3 activation in NPM1-mutated leukemia"

Article Title: INPP4B promotes cell survival via SGK3 activation in NPM1-mutated leukemia

Journal: Journal of Experimental & Clinical Cancer Research : CR

doi: 10.1186/s13046-018-0675-9

INPP4B is upregulated by NPM1-mA in leukemia cells via ERK/Ets-1 signaling. ( a ) qRT-PCR analysis of INPP4B mRNA expression, ( b ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. ( c ) qRT-PCR analysis of INPP4B mRNA expression, ( d ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the THP-1 and K562 cells transduced with the plasmids expressing NPM1-wt or NPM1-mA. ( e ) qRT-PCR analysis of INPP4B mRNA expression, ( f ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3, p-Ets-1 and Ets-1 from the OCI-AML3 cells transfected with the control siRNA or siEts-1. ( g ) Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 and SGK3, ( h ) qRT-PCR analysis of INPP4B mRNA expression from the OCI-AML3 cells treated with different concentration of PD98059 (0, 10, 20 and 40 μM). i Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. Proteins were quantified using image software and normalized against β-actin. Data were represented as mean ± s.d. of three individual experiments. * p
Figure Legend Snippet: INPP4B is upregulated by NPM1-mA in leukemia cells via ERK/Ets-1 signaling. ( a ) qRT-PCR analysis of INPP4B mRNA expression, ( b ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. ( c ) qRT-PCR analysis of INPP4B mRNA expression, ( d ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the THP-1 and K562 cells transduced with the plasmids expressing NPM1-wt or NPM1-mA. ( e ) qRT-PCR analysis of INPP4B mRNA expression, ( f ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3, p-Ets-1 and Ets-1 from the OCI-AML3 cells transfected with the control siRNA or siEts-1. ( g ) Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 and SGK3, ( h ) qRT-PCR analysis of INPP4B mRNA expression from the OCI-AML3 cells treated with different concentration of PD98059 (0, 10, 20 and 40 μM). i Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. Proteins were quantified using image software and normalized against β-actin. Data were represented as mean ± s.d. of three individual experiments. * p

Techniques Used: Quantitative RT-PCR, Expressing, Western Blot, Transduction, Transfection, Concentration Assay, Software

NPM1-mA-mediated INPP4B upregulation promotes cell proliferation in OCI-AML3 cells. The NPM1-mA-silenced OCI-AML3 cells were subjected to ( a ) CCK8 assays and ( b ) colony forming assays. c The NPM1-mA-silenced OCI-AML3 cells were transfected with the pEAK-Flag/INPP4B plasmids, western blotting analysis of INPP4B and NPM1-mA. Proteins were quantified using image software and normalized against β-actin. d CCK-8 assay analysis of cell proliferation in NPM1-mA-silenced OCI-AML3 cells, followed by Flag-INPP4B introduction. Data were represented as mean ± s.d. of three individual experiments. * p
Figure Legend Snippet: NPM1-mA-mediated INPP4B upregulation promotes cell proliferation in OCI-AML3 cells. The NPM1-mA-silenced OCI-AML3 cells were subjected to ( a ) CCK8 assays and ( b ) colony forming assays. c The NPM1-mA-silenced OCI-AML3 cells were transfected with the pEAK-Flag/INPP4B plasmids, western blotting analysis of INPP4B and NPM1-mA. Proteins were quantified using image software and normalized against β-actin. d CCK-8 assay analysis of cell proliferation in NPM1-mA-silenced OCI-AML3 cells, followed by Flag-INPP4B introduction. Data were represented as mean ± s.d. of three individual experiments. * p

Techniques Used: Transfection, Western Blot, Software, CCK-8 Assay

High expression of INPP4B is associated with poor survival outcome in NPM1-mutated leukemia. Kaplan-Meier survival data of 153 AML patients were used to analysis ( a ) OS and ( b ) EFS curves according to INPP4B levels. c Heatmap of 38 primary AML samples with NPM1 mutation from TCGA dataset in which INPP4B expression was aligned with patient event (Survival or Death). Kaplan-Meier survival data of patients with NPM1-mutated AML were used to analysis ( d ) OS and ( e ) EFS curves according to INPP4B levels. f Schematic diagram describing the functional significance of INPP4B in the NPM1-mutated leukemia cells. INPP4B promotes leukemia cell survival in a SGK3-dependent and AKT-independent manner. The expression of INPP4B partially upregulated by NPM1-mA is due to ERK/Ets-1 signaling
Figure Legend Snippet: High expression of INPP4B is associated with poor survival outcome in NPM1-mutated leukemia. Kaplan-Meier survival data of 153 AML patients were used to analysis ( a ) OS and ( b ) EFS curves according to INPP4B levels. c Heatmap of 38 primary AML samples with NPM1 mutation from TCGA dataset in which INPP4B expression was aligned with patient event (Survival or Death). Kaplan-Meier survival data of patients with NPM1-mutated AML were used to analysis ( d ) OS and ( e ) EFS curves according to INPP4B levels. f Schematic diagram describing the functional significance of INPP4B in the NPM1-mutated leukemia cells. INPP4B promotes leukemia cell survival in a SGK3-dependent and AKT-independent manner. The expression of INPP4B partially upregulated by NPM1-mA is due to ERK/Ets-1 signaling

Techniques Used: Expressing, Mutagenesis, Functional Assay

High expression levels of INPP4B in leukemia cells with the NPM1 mutation. a RNA-seq mRNA expression data from the TCGA database were used to compare INPP4B expression between AML patients with ( n = 41) and without NPM1 mutation ( n = 130). * p
Figure Legend Snippet: High expression levels of INPP4B in leukemia cells with the NPM1 mutation. a RNA-seq mRNA expression data from the TCGA database were used to compare INPP4B expression between AML patients with ( n = 41) and without NPM1 mutation ( n = 130). * p

Techniques Used: Expressing, Mutagenesis, RNA Sequencing Assay

10) Product Images from "INPP4B promotes cell survival via SGK3 activation in NPM1-mutated leukemia"

Article Title: INPP4B promotes cell survival via SGK3 activation in NPM1-mutated leukemia

Journal: Journal of Experimental & Clinical Cancer Research : CR

doi: 10.1186/s13046-018-0675-9

INPP4B is upregulated by NPM1-mA in leukemia cells via ERK/Ets-1 signaling. ( a ) qRT-PCR analysis of INPP4B mRNA expression, ( b ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. ( c ) qRT-PCR analysis of INPP4B mRNA expression, ( d ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the THP-1 and K562 cells transduced with the plasmids expressing NPM1-wt or NPM1-mA. ( e ) qRT-PCR analysis of INPP4B mRNA expression, ( f ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3, p-Ets-1 and Ets-1 from the OCI-AML3 cells transfected with the control siRNA or siEts-1. ( g ) Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 and SGK3, ( h ) qRT-PCR analysis of INPP4B mRNA expression from the OCI-AML3 cells treated with different concentration of PD98059 (0, 10, 20 and 40 μM). i Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. Proteins were quantified using image software and normalized against β-actin. Data were represented as mean ± s.d. of three individual experiments. * p
Figure Legend Snippet: INPP4B is upregulated by NPM1-mA in leukemia cells via ERK/Ets-1 signaling. ( a ) qRT-PCR analysis of INPP4B mRNA expression, ( b ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. ( c ) qRT-PCR analysis of INPP4B mRNA expression, ( d ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the THP-1 and K562 cells transduced with the plasmids expressing NPM1-wt or NPM1-mA. ( e ) qRT-PCR analysis of INPP4B mRNA expression, ( f ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3, p-Ets-1 and Ets-1 from the OCI-AML3 cells transfected with the control siRNA or siEts-1. ( g ) Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 and SGK3, ( h ) qRT-PCR analysis of INPP4B mRNA expression from the OCI-AML3 cells treated with different concentration of PD98059 (0, 10, 20 and 40 μM). i Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. Proteins were quantified using image software and normalized against β-actin. Data were represented as mean ± s.d. of three individual experiments. * p

Techniques Used: Quantitative RT-PCR, Expressing, Western Blot, Transduction, Transfection, Concentration Assay, Software

NPM1-mA-mediated INPP4B upregulation promotes cell proliferation in OCI-AML3 cells. The NPM1-mA-silenced OCI-AML3 cells were subjected to ( a ) CCK8 assays and ( b ) colony forming assays. c The NPM1-mA-silenced OCI-AML3 cells were transfected with the pEAK-Flag/INPP4B plasmids, western blotting analysis of INPP4B and NPM1-mA. Proteins were quantified using image software and normalized against β-actin. d CCK-8 assay analysis of cell proliferation in NPM1-mA-silenced OCI-AML3 cells, followed by Flag-INPP4B introduction. Data were represented as mean ± s.d. of three individual experiments. * p
Figure Legend Snippet: NPM1-mA-mediated INPP4B upregulation promotes cell proliferation in OCI-AML3 cells. The NPM1-mA-silenced OCI-AML3 cells were subjected to ( a ) CCK8 assays and ( b ) colony forming assays. c The NPM1-mA-silenced OCI-AML3 cells were transfected with the pEAK-Flag/INPP4B plasmids, western blotting analysis of INPP4B and NPM1-mA. Proteins were quantified using image software and normalized against β-actin. d CCK-8 assay analysis of cell proliferation in NPM1-mA-silenced OCI-AML3 cells, followed by Flag-INPP4B introduction. Data were represented as mean ± s.d. of three individual experiments. * p

Techniques Used: Transfection, Western Blot, Software, CCK-8 Assay

High expression of INPP4B is associated with poor survival outcome in NPM1-mutated leukemia. Kaplan-Meier survival data of 153 AML patients were used to analysis ( a ) OS and ( b ) EFS curves according to INPP4B levels. c Heatmap of 38 primary AML samples with NPM1 mutation from TCGA dataset in which INPP4B expression was aligned with patient event (Survival or Death). Kaplan-Meier survival data of patients with NPM1-mutated AML were used to analysis ( d ) OS and ( e ) EFS curves according to INPP4B levels. f Schematic diagram describing the functional significance of INPP4B in the NPM1-mutated leukemia cells. INPP4B promotes leukemia cell survival in a SGK3-dependent and AKT-independent manner. The expression of INPP4B partially upregulated by NPM1-mA is due to ERK/Ets-1 signaling
Figure Legend Snippet: High expression of INPP4B is associated with poor survival outcome in NPM1-mutated leukemia. Kaplan-Meier survival data of 153 AML patients were used to analysis ( a ) OS and ( b ) EFS curves according to INPP4B levels. c Heatmap of 38 primary AML samples with NPM1 mutation from TCGA dataset in which INPP4B expression was aligned with patient event (Survival or Death). Kaplan-Meier survival data of patients with NPM1-mutated AML were used to analysis ( d ) OS and ( e ) EFS curves according to INPP4B levels. f Schematic diagram describing the functional significance of INPP4B in the NPM1-mutated leukemia cells. INPP4B promotes leukemia cell survival in a SGK3-dependent and AKT-independent manner. The expression of INPP4B partially upregulated by NPM1-mA is due to ERK/Ets-1 signaling

Techniques Used: Expressing, Mutagenesis, Functional Assay

High expression levels of INPP4B in leukemia cells with the NPM1 mutation. a RNA-seq mRNA expression data from the TCGA database were used to compare INPP4B expression between AML patients with ( n = 41) and without NPM1 mutation ( n = 130). * p
Figure Legend Snippet: High expression levels of INPP4B in leukemia cells with the NPM1 mutation. a RNA-seq mRNA expression data from the TCGA database were used to compare INPP4B expression between AML patients with ( n = 41) and without NPM1 mutation ( n = 130). * p

Techniques Used: Expressing, Mutagenesis, RNA Sequencing Assay

11) Product Images from "INPP4B promotes cell survival via SGK3 activation in NPM1-mutated leukemia"

Article Title: INPP4B promotes cell survival via SGK3 activation in NPM1-mutated leukemia

Journal: Journal of Experimental & Clinical Cancer Research : CR

doi: 10.1186/s13046-018-0675-9

INPP4B is upregulated by NPM1-mA in leukemia cells via ERK/Ets-1 signaling. ( a ) qRT-PCR analysis of INPP4B mRNA expression, ( b ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. ( c ) qRT-PCR analysis of INPP4B mRNA expression, ( d ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the THP-1 and K562 cells transduced with the plasmids expressing NPM1-wt or NPM1-mA. ( e ) qRT-PCR analysis of INPP4B mRNA expression, ( f ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3, p-Ets-1 and Ets-1 from the OCI-AML3 cells transfected with the control siRNA or siEts-1. ( g ) Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 and SGK3, ( h ) qRT-PCR analysis of INPP4B mRNA expression from the OCI-AML3 cells treated with different concentration of PD98059 (0, 10, 20 and 40 μM). i Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. Proteins were quantified using image software and normalized against β-actin. Data were represented as mean ± s.d. of three individual experiments. * p
Figure Legend Snippet: INPP4B is upregulated by NPM1-mA in leukemia cells via ERK/Ets-1 signaling. ( a ) qRT-PCR analysis of INPP4B mRNA expression, ( b ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. ( c ) qRT-PCR analysis of INPP4B mRNA expression, ( d ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the THP-1 and K562 cells transduced with the plasmids expressing NPM1-wt or NPM1-mA. ( e ) qRT-PCR analysis of INPP4B mRNA expression, ( f ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3, p-Ets-1 and Ets-1 from the OCI-AML3 cells transfected with the control siRNA or siEts-1. ( g ) Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 and SGK3, ( h ) qRT-PCR analysis of INPP4B mRNA expression from the OCI-AML3 cells treated with different concentration of PD98059 (0, 10, 20 and 40 μM). i Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. Proteins were quantified using image software and normalized against β-actin. Data were represented as mean ± s.d. of three individual experiments. * p

Techniques Used: Quantitative RT-PCR, Expressing, Western Blot, Transduction, Transfection, Concentration Assay, Software

NPM1-mA-mediated INPP4B upregulation promotes cell proliferation in OCI-AML3 cells. The NPM1-mA-silenced OCI-AML3 cells were subjected to ( a ) CCK8 assays and ( b ) colony forming assays. c The NPM1-mA-silenced OCI-AML3 cells were transfected with the pEAK-Flag/INPP4B plasmids, western blotting analysis of INPP4B and NPM1-mA. Proteins were quantified using image software and normalized against β-actin. d CCK-8 assay analysis of cell proliferation in NPM1-mA-silenced OCI-AML3 cells, followed by Flag-INPP4B introduction. Data were represented as mean ± s.d. of three individual experiments. * p
Figure Legend Snippet: NPM1-mA-mediated INPP4B upregulation promotes cell proliferation in OCI-AML3 cells. The NPM1-mA-silenced OCI-AML3 cells were subjected to ( a ) CCK8 assays and ( b ) colony forming assays. c The NPM1-mA-silenced OCI-AML3 cells were transfected with the pEAK-Flag/INPP4B plasmids, western blotting analysis of INPP4B and NPM1-mA. Proteins were quantified using image software and normalized against β-actin. d CCK-8 assay analysis of cell proliferation in NPM1-mA-silenced OCI-AML3 cells, followed by Flag-INPP4B introduction. Data were represented as mean ± s.d. of three individual experiments. * p

Techniques Used: Transfection, Western Blot, Software, CCK-8 Assay

High expression of INPP4B is associated with poor survival outcome in NPM1-mutated leukemia. Kaplan-Meier survival data of 153 AML patients were used to analysis ( a ) OS and ( b ) EFS curves according to INPP4B levels. c Heatmap of 38 primary AML samples with NPM1 mutation from TCGA dataset in which INPP4B expression was aligned with patient event (Survival or Death). Kaplan-Meier survival data of patients with NPM1-mutated AML were used to analysis ( d ) OS and ( e ) EFS curves according to INPP4B levels. f Schematic diagram describing the functional significance of INPP4B in the NPM1-mutated leukemia cells. INPP4B promotes leukemia cell survival in a SGK3-dependent and AKT-independent manner. The expression of INPP4B partially upregulated by NPM1-mA is due to ERK/Ets-1 signaling
Figure Legend Snippet: High expression of INPP4B is associated with poor survival outcome in NPM1-mutated leukemia. Kaplan-Meier survival data of 153 AML patients were used to analysis ( a ) OS and ( b ) EFS curves according to INPP4B levels. c Heatmap of 38 primary AML samples with NPM1 mutation from TCGA dataset in which INPP4B expression was aligned with patient event (Survival or Death). Kaplan-Meier survival data of patients with NPM1-mutated AML were used to analysis ( d ) OS and ( e ) EFS curves according to INPP4B levels. f Schematic diagram describing the functional significance of INPP4B in the NPM1-mutated leukemia cells. INPP4B promotes leukemia cell survival in a SGK3-dependent and AKT-independent manner. The expression of INPP4B partially upregulated by NPM1-mA is due to ERK/Ets-1 signaling

Techniques Used: Expressing, Mutagenesis, Functional Assay

High expression levels of INPP4B in leukemia cells with the NPM1 mutation. a RNA-seq mRNA expression data from the TCGA database were used to compare INPP4B expression between AML patients with ( n = 41) and without NPM1 mutation ( n = 130). * p
Figure Legend Snippet: High expression levels of INPP4B in leukemia cells with the NPM1 mutation. a RNA-seq mRNA expression data from the TCGA database were used to compare INPP4B expression between AML patients with ( n = 41) and without NPM1 mutation ( n = 130). * p

Techniques Used: Expressing, Mutagenesis, RNA Sequencing Assay

12) Product Images from "INPP4B promotes cell survival via SGK3 activation in NPM1-mutated leukemia"

Article Title: INPP4B promotes cell survival via SGK3 activation in NPM1-mutated leukemia

Journal: Journal of Experimental & Clinical Cancer Research : CR

doi: 10.1186/s13046-018-0675-9

INPP4B is upregulated by NPM1-mA in leukemia cells via ERK/Ets-1 signaling. ( a ) qRT-PCR analysis of INPP4B mRNA expression, ( b ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. ( c ) qRT-PCR analysis of INPP4B mRNA expression, ( d ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the THP-1 and K562 cells transduced with the plasmids expressing NPM1-wt or NPM1-mA. ( e ) qRT-PCR analysis of INPP4B mRNA expression, ( f ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3, p-Ets-1 and Ets-1 from the OCI-AML3 cells transfected with the control siRNA or siEts-1. ( g ) Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 and SGK3, ( h ) qRT-PCR analysis of INPP4B mRNA expression from the OCI-AML3 cells treated with different concentration of PD98059 (0, 10, 20 and 40 μM). i Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. Proteins were quantified using image software and normalized against β-actin. Data were represented as mean ± s.d. of three individual experiments. * p
Figure Legend Snippet: INPP4B is upregulated by NPM1-mA in leukemia cells via ERK/Ets-1 signaling. ( a ) qRT-PCR analysis of INPP4B mRNA expression, ( b ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. ( c ) qRT-PCR analysis of INPP4B mRNA expression, ( d ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the THP-1 and K562 cells transduced with the plasmids expressing NPM1-wt or NPM1-mA. ( e ) qRT-PCR analysis of INPP4B mRNA expression, ( f ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3, p-Ets-1 and Ets-1 from the OCI-AML3 cells transfected with the control siRNA or siEts-1. ( g ) Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 and SGK3, ( h ) qRT-PCR analysis of INPP4B mRNA expression from the OCI-AML3 cells treated with different concentration of PD98059 (0, 10, 20 and 40 μM). i Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. Proteins were quantified using image software and normalized against β-actin. Data were represented as mean ± s.d. of three individual experiments. * p

Techniques Used: Quantitative RT-PCR, Expressing, Western Blot, Transduction, Transfection, Concentration Assay, Software

NPM1-mA-mediated INPP4B upregulation promotes cell proliferation in OCI-AML3 cells. The NPM1-mA-silenced OCI-AML3 cells were subjected to ( a ) CCK8 assays and ( b ) colony forming assays. c The NPM1-mA-silenced OCI-AML3 cells were transfected with the pEAK-Flag/INPP4B plasmids, western blotting analysis of INPP4B and NPM1-mA. Proteins were quantified using image software and normalized against β-actin. d CCK-8 assay analysis of cell proliferation in NPM1-mA-silenced OCI-AML3 cells, followed by Flag-INPP4B introduction. Data were represented as mean ± s.d. of three individual experiments. * p
Figure Legend Snippet: NPM1-mA-mediated INPP4B upregulation promotes cell proliferation in OCI-AML3 cells. The NPM1-mA-silenced OCI-AML3 cells were subjected to ( a ) CCK8 assays and ( b ) colony forming assays. c The NPM1-mA-silenced OCI-AML3 cells were transfected with the pEAK-Flag/INPP4B plasmids, western blotting analysis of INPP4B and NPM1-mA. Proteins were quantified using image software and normalized against β-actin. d CCK-8 assay analysis of cell proliferation in NPM1-mA-silenced OCI-AML3 cells, followed by Flag-INPP4B introduction. Data were represented as mean ± s.d. of three individual experiments. * p

Techniques Used: Transfection, Western Blot, Software, CCK-8 Assay

High expression of INPP4B is associated with poor survival outcome in NPM1-mutated leukemia. Kaplan-Meier survival data of 153 AML patients were used to analysis ( a ) OS and ( b ) EFS curves according to INPP4B levels. c Heatmap of 38 primary AML samples with NPM1 mutation from TCGA dataset in which INPP4B expression was aligned with patient event (Survival or Death). Kaplan-Meier survival data of patients with NPM1-mutated AML were used to analysis ( d ) OS and ( e ) EFS curves according to INPP4B levels. f Schematic diagram describing the functional significance of INPP4B in the NPM1-mutated leukemia cells. INPP4B promotes leukemia cell survival in a SGK3-dependent and AKT-independent manner. The expression of INPP4B partially upregulated by NPM1-mA is due to ERK/Ets-1 signaling
Figure Legend Snippet: High expression of INPP4B is associated with poor survival outcome in NPM1-mutated leukemia. Kaplan-Meier survival data of 153 AML patients were used to analysis ( a ) OS and ( b ) EFS curves according to INPP4B levels. c Heatmap of 38 primary AML samples with NPM1 mutation from TCGA dataset in which INPP4B expression was aligned with patient event (Survival or Death). Kaplan-Meier survival data of patients with NPM1-mutated AML were used to analysis ( d ) OS and ( e ) EFS curves according to INPP4B levels. f Schematic diagram describing the functional significance of INPP4B in the NPM1-mutated leukemia cells. INPP4B promotes leukemia cell survival in a SGK3-dependent and AKT-independent manner. The expression of INPP4B partially upregulated by NPM1-mA is due to ERK/Ets-1 signaling

Techniques Used: Expressing, Mutagenesis, Functional Assay

High expression levels of INPP4B in leukemia cells with the NPM1 mutation. a RNA-seq mRNA expression data from the TCGA database were used to compare INPP4B expression between AML patients with ( n = 41) and without NPM1 mutation ( n = 130). * p
Figure Legend Snippet: High expression levels of INPP4B in leukemia cells with the NPM1 mutation. a RNA-seq mRNA expression data from the TCGA database were used to compare INPP4B expression between AML patients with ( n = 41) and without NPM1 mutation ( n = 130). * p

Techniques Used: Expressing, Mutagenesis, RNA Sequencing Assay

13) Product Images from "INPP4B promotes cell survival via SGK3 activation in NPM1-mutated leukemia"

Article Title: INPP4B promotes cell survival via SGK3 activation in NPM1-mutated leukemia

Journal: Journal of Experimental & Clinical Cancer Research : CR

doi: 10.1186/s13046-018-0675-9

INPP4B is upregulated by NPM1-mA in leukemia cells via ERK/Ets-1 signaling. ( a ) qRT-PCR analysis of INPP4B mRNA expression, ( b ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. ( c ) qRT-PCR analysis of INPP4B mRNA expression, ( d ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the THP-1 and K562 cells transduced with the plasmids expressing NPM1-wt or NPM1-mA. ( e ) qRT-PCR analysis of INPP4B mRNA expression, ( f ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3, p-Ets-1 and Ets-1 from the OCI-AML3 cells transfected with the control siRNA or siEts-1. ( g ) Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 and SGK3, ( h ) qRT-PCR analysis of INPP4B mRNA expression from the OCI-AML3 cells treated with different concentration of PD98059 (0, 10, 20 and 40 μM). i Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. Proteins were quantified using image software and normalized against β-actin. Data were represented as mean ± s.d. of three individual experiments. * p
Figure Legend Snippet: INPP4B is upregulated by NPM1-mA in leukemia cells via ERK/Ets-1 signaling. ( a ) qRT-PCR analysis of INPP4B mRNA expression, ( b ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. ( c ) qRT-PCR analysis of INPP4B mRNA expression, ( d ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the THP-1 and K562 cells transduced with the plasmids expressing NPM1-wt or NPM1-mA. ( e ) qRT-PCR analysis of INPP4B mRNA expression, ( f ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3, p-Ets-1 and Ets-1 from the OCI-AML3 cells transfected with the control siRNA or siEts-1. ( g ) Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 and SGK3, ( h ) qRT-PCR analysis of INPP4B mRNA expression from the OCI-AML3 cells treated with different concentration of PD98059 (0, 10, 20 and 40 μM). i Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. Proteins were quantified using image software and normalized against β-actin. Data were represented as mean ± s.d. of three individual experiments. * p

Techniques Used: Quantitative RT-PCR, Expressing, Western Blot, Transduction, Transfection, Concentration Assay, Software

NPM1-mA-mediated INPP4B upregulation promotes cell proliferation in OCI-AML3 cells. The NPM1-mA-silenced OCI-AML3 cells were subjected to ( a ) CCK8 assays and ( b ) colony forming assays. c The NPM1-mA-silenced OCI-AML3 cells were transfected with the pEAK-Flag/INPP4B plasmids, western blotting analysis of INPP4B and NPM1-mA. Proteins were quantified using image software and normalized against β-actin. d CCK-8 assay analysis of cell proliferation in NPM1-mA-silenced OCI-AML3 cells, followed by Flag-INPP4B introduction. Data were represented as mean ± s.d. of three individual experiments. * p
Figure Legend Snippet: NPM1-mA-mediated INPP4B upregulation promotes cell proliferation in OCI-AML3 cells. The NPM1-mA-silenced OCI-AML3 cells were subjected to ( a ) CCK8 assays and ( b ) colony forming assays. c The NPM1-mA-silenced OCI-AML3 cells were transfected with the pEAK-Flag/INPP4B plasmids, western blotting analysis of INPP4B and NPM1-mA. Proteins were quantified using image software and normalized against β-actin. d CCK-8 assay analysis of cell proliferation in NPM1-mA-silenced OCI-AML3 cells, followed by Flag-INPP4B introduction. Data were represented as mean ± s.d. of three individual experiments. * p

Techniques Used: Transfection, Western Blot, Software, CCK-8 Assay

High expression of INPP4B is associated with poor survival outcome in NPM1-mutated leukemia. Kaplan-Meier survival data of 153 AML patients were used to analysis ( a ) OS and ( b ) EFS curves according to INPP4B levels. c Heatmap of 38 primary AML samples with NPM1 mutation from TCGA dataset in which INPP4B expression was aligned with patient event (Survival or Death). Kaplan-Meier survival data of patients with NPM1-mutated AML were used to analysis ( d ) OS and ( e ) EFS curves according to INPP4B levels. f Schematic diagram describing the functional significance of INPP4B in the NPM1-mutated leukemia cells. INPP4B promotes leukemia cell survival in a SGK3-dependent and AKT-independent manner. The expression of INPP4B partially upregulated by NPM1-mA is due to ERK/Ets-1 signaling
Figure Legend Snippet: High expression of INPP4B is associated with poor survival outcome in NPM1-mutated leukemia. Kaplan-Meier survival data of 153 AML patients were used to analysis ( a ) OS and ( b ) EFS curves according to INPP4B levels. c Heatmap of 38 primary AML samples with NPM1 mutation from TCGA dataset in which INPP4B expression was aligned with patient event (Survival or Death). Kaplan-Meier survival data of patients with NPM1-mutated AML were used to analysis ( d ) OS and ( e ) EFS curves according to INPP4B levels. f Schematic diagram describing the functional significance of INPP4B in the NPM1-mutated leukemia cells. INPP4B promotes leukemia cell survival in a SGK3-dependent and AKT-independent manner. The expression of INPP4B partially upregulated by NPM1-mA is due to ERK/Ets-1 signaling

Techniques Used: Expressing, Mutagenesis, Functional Assay

High expression levels of INPP4B in leukemia cells with the NPM1 mutation. a RNA-seq mRNA expression data from the TCGA database were used to compare INPP4B expression between AML patients with ( n = 41) and without NPM1 mutation ( n = 130). * p
Figure Legend Snippet: High expression levels of INPP4B in leukemia cells with the NPM1 mutation. a RNA-seq mRNA expression data from the TCGA database were used to compare INPP4B expression between AML patients with ( n = 41) and without NPM1 mutation ( n = 130). * p

Techniques Used: Expressing, Mutagenesis, RNA Sequencing Assay

14) Product Images from "INPP4B promotes cell survival via SGK3 activation in NPM1-mutated leukemia"

Article Title: INPP4B promotes cell survival via SGK3 activation in NPM1-mutated leukemia

Journal: Journal of Experimental & Clinical Cancer Research : CR

doi: 10.1186/s13046-018-0675-9

INPP4B is upregulated by NPM1-mA in leukemia cells via ERK/Ets-1 signaling. ( a ) qRT-PCR analysis of INPP4B mRNA expression, ( b ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. ( c ) qRT-PCR analysis of INPP4B mRNA expression, ( d ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the THP-1 and K562 cells transduced with the plasmids expressing NPM1-wt or NPM1-mA. ( e ) qRT-PCR analysis of INPP4B mRNA expression, ( f ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3, p-Ets-1 and Ets-1 from the OCI-AML3 cells transfected with the control siRNA or siEts-1. ( g ) Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 and SGK3, ( h ) qRT-PCR analysis of INPP4B mRNA expression from the OCI-AML3 cells treated with different concentration of PD98059 (0, 10, 20 and 40 μM). i Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. Proteins were quantified using image software and normalized against β-actin. Data were represented as mean ± s.d. of three individual experiments. * p
Figure Legend Snippet: INPP4B is upregulated by NPM1-mA in leukemia cells via ERK/Ets-1 signaling. ( a ) qRT-PCR analysis of INPP4B mRNA expression, ( b ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. ( c ) qRT-PCR analysis of INPP4B mRNA expression, ( d ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the THP-1 and K562 cells transduced with the plasmids expressing NPM1-wt or NPM1-mA. ( e ) qRT-PCR analysis of INPP4B mRNA expression, ( f ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3, p-Ets-1 and Ets-1 from the OCI-AML3 cells transfected with the control siRNA or siEts-1. ( g ) Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 and SGK3, ( h ) qRT-PCR analysis of INPP4B mRNA expression from the OCI-AML3 cells treated with different concentration of PD98059 (0, 10, 20 and 40 μM). i Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. Proteins were quantified using image software and normalized against β-actin. Data were represented as mean ± s.d. of three individual experiments. * p

Techniques Used: Quantitative RT-PCR, Expressing, Western Blot, Transduction, Transfection, Concentration Assay, Software

NPM1-mA-mediated INPP4B upregulation promotes cell proliferation in OCI-AML3 cells. The NPM1-mA-silenced OCI-AML3 cells were subjected to ( a ) CCK8 assays and ( b ) colony forming assays. c The NPM1-mA-silenced OCI-AML3 cells were transfected with the pEAK-Flag/INPP4B plasmids, western blotting analysis of INPP4B and NPM1-mA. Proteins were quantified using image software and normalized against β-actin. d CCK-8 assay analysis of cell proliferation in NPM1-mA-silenced OCI-AML3 cells, followed by Flag-INPP4B introduction. Data were represented as mean ± s.d. of three individual experiments. * p
Figure Legend Snippet: NPM1-mA-mediated INPP4B upregulation promotes cell proliferation in OCI-AML3 cells. The NPM1-mA-silenced OCI-AML3 cells were subjected to ( a ) CCK8 assays and ( b ) colony forming assays. c The NPM1-mA-silenced OCI-AML3 cells were transfected with the pEAK-Flag/INPP4B plasmids, western blotting analysis of INPP4B and NPM1-mA. Proteins were quantified using image software and normalized against β-actin. d CCK-8 assay analysis of cell proliferation in NPM1-mA-silenced OCI-AML3 cells, followed by Flag-INPP4B introduction. Data were represented as mean ± s.d. of three individual experiments. * p

Techniques Used: Transfection, Western Blot, Software, CCK-8 Assay

High expression of INPP4B is associated with poor survival outcome in NPM1-mutated leukemia. Kaplan-Meier survival data of 153 AML patients were used to analysis ( a ) OS and ( b ) EFS curves according to INPP4B levels. c Heatmap of 38 primary AML samples with NPM1 mutation from TCGA dataset in which INPP4B expression was aligned with patient event (Survival or Death). Kaplan-Meier survival data of patients with NPM1-mutated AML were used to analysis ( d ) OS and ( e ) EFS curves according to INPP4B levels. f Schematic diagram describing the functional significance of INPP4B in the NPM1-mutated leukemia cells. INPP4B promotes leukemia cell survival in a SGK3-dependent and AKT-independent manner. The expression of INPP4B partially upregulated by NPM1-mA is due to ERK/Ets-1 signaling
Figure Legend Snippet: High expression of INPP4B is associated with poor survival outcome in NPM1-mutated leukemia. Kaplan-Meier survival data of 153 AML patients were used to analysis ( a ) OS and ( b ) EFS curves according to INPP4B levels. c Heatmap of 38 primary AML samples with NPM1 mutation from TCGA dataset in which INPP4B expression was aligned with patient event (Survival or Death). Kaplan-Meier survival data of patients with NPM1-mutated AML were used to analysis ( d ) OS and ( e ) EFS curves according to INPP4B levels. f Schematic diagram describing the functional significance of INPP4B in the NPM1-mutated leukemia cells. INPP4B promotes leukemia cell survival in a SGK3-dependent and AKT-independent manner. The expression of INPP4B partially upregulated by NPM1-mA is due to ERK/Ets-1 signaling

Techniques Used: Expressing, Mutagenesis, Functional Assay

High expression levels of INPP4B in leukemia cells with the NPM1 mutation. a RNA-seq mRNA expression data from the TCGA database were used to compare INPP4B expression between AML patients with ( n = 41) and without NPM1 mutation ( n = 130). * p
Figure Legend Snippet: High expression levels of INPP4B in leukemia cells with the NPM1 mutation. a RNA-seq mRNA expression data from the TCGA database were used to compare INPP4B expression between AML patients with ( n = 41) and without NPM1 mutation ( n = 130). * p

Techniques Used: Expressing, Mutagenesis, RNA Sequencing Assay

15) Product Images from "INPP4B promotes cell survival via SGK3 activation in NPM1-mutated leukemia"

Article Title: INPP4B promotes cell survival via SGK3 activation in NPM1-mutated leukemia

Journal: Journal of Experimental & Clinical Cancer Research : CR

doi: 10.1186/s13046-018-0675-9

INPP4B is upregulated by NPM1-mA in leukemia cells via ERK/Ets-1 signaling. ( a ) qRT-PCR analysis of INPP4B mRNA expression, ( b ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. ( c ) qRT-PCR analysis of INPP4B mRNA expression, ( d ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the THP-1 and K562 cells transduced with the plasmids expressing NPM1-wt or NPM1-mA. ( e ) qRT-PCR analysis of INPP4B mRNA expression, ( f ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3, p-Ets-1 and Ets-1 from the OCI-AML3 cells transfected with the control siRNA or siEts-1. ( g ) Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 and SGK3, ( h ) qRT-PCR analysis of INPP4B mRNA expression from the OCI-AML3 cells treated with different concentration of PD98059 (0, 10, 20 and 40 μM). i Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. Proteins were quantified using image software and normalized against β-actin. Data were represented as mean ± s.d. of three individual experiments. * p
Figure Legend Snippet: INPP4B is upregulated by NPM1-mA in leukemia cells via ERK/Ets-1 signaling. ( a ) qRT-PCR analysis of INPP4B mRNA expression, ( b ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. ( c ) qRT-PCR analysis of INPP4B mRNA expression, ( d ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the THP-1 and K562 cells transduced with the plasmids expressing NPM1-wt or NPM1-mA. ( e ) qRT-PCR analysis of INPP4B mRNA expression, ( f ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3, p-Ets-1 and Ets-1 from the OCI-AML3 cells transfected with the control siRNA or siEts-1. ( g ) Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 and SGK3, ( h ) qRT-PCR analysis of INPP4B mRNA expression from the OCI-AML3 cells treated with different concentration of PD98059 (0, 10, 20 and 40 μM). i Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. Proteins were quantified using image software and normalized against β-actin. Data were represented as mean ± s.d. of three individual experiments. * p

Techniques Used: Quantitative RT-PCR, Expressing, Western Blot, Transduction, Transfection, Concentration Assay, Software

NPM1-mA-mediated INPP4B upregulation promotes cell proliferation in OCI-AML3 cells. The NPM1-mA-silenced OCI-AML3 cells were subjected to ( a ) CCK8 assays and ( b ) colony forming assays. c The NPM1-mA-silenced OCI-AML3 cells were transfected with the pEAK-Flag/INPP4B plasmids, western blotting analysis of INPP4B and NPM1-mA. Proteins were quantified using image software and normalized against β-actin. d CCK-8 assay analysis of cell proliferation in NPM1-mA-silenced OCI-AML3 cells, followed by Flag-INPP4B introduction. Data were represented as mean ± s.d. of three individual experiments. * p
Figure Legend Snippet: NPM1-mA-mediated INPP4B upregulation promotes cell proliferation in OCI-AML3 cells. The NPM1-mA-silenced OCI-AML3 cells were subjected to ( a ) CCK8 assays and ( b ) colony forming assays. c The NPM1-mA-silenced OCI-AML3 cells were transfected with the pEAK-Flag/INPP4B plasmids, western blotting analysis of INPP4B and NPM1-mA. Proteins were quantified using image software and normalized against β-actin. d CCK-8 assay analysis of cell proliferation in NPM1-mA-silenced OCI-AML3 cells, followed by Flag-INPP4B introduction. Data were represented as mean ± s.d. of three individual experiments. * p

Techniques Used: Transfection, Western Blot, Software, CCK-8 Assay

High expression of INPP4B is associated with poor survival outcome in NPM1-mutated leukemia. Kaplan-Meier survival data of 153 AML patients were used to analysis ( a ) OS and ( b ) EFS curves according to INPP4B levels. c Heatmap of 38 primary AML samples with NPM1 mutation from TCGA dataset in which INPP4B expression was aligned with patient event (Survival or Death). Kaplan-Meier survival data of patients with NPM1-mutated AML were used to analysis ( d ) OS and ( e ) EFS curves according to INPP4B levels. f Schematic diagram describing the functional significance of INPP4B in the NPM1-mutated leukemia cells. INPP4B promotes leukemia cell survival in a SGK3-dependent and AKT-independent manner. The expression of INPP4B partially upregulated by NPM1-mA is due to ERK/Ets-1 signaling
Figure Legend Snippet: High expression of INPP4B is associated with poor survival outcome in NPM1-mutated leukemia. Kaplan-Meier survival data of 153 AML patients were used to analysis ( a ) OS and ( b ) EFS curves according to INPP4B levels. c Heatmap of 38 primary AML samples with NPM1 mutation from TCGA dataset in which INPP4B expression was aligned with patient event (Survival or Death). Kaplan-Meier survival data of patients with NPM1-mutated AML were used to analysis ( d ) OS and ( e ) EFS curves according to INPP4B levels. f Schematic diagram describing the functional significance of INPP4B in the NPM1-mutated leukemia cells. INPP4B promotes leukemia cell survival in a SGK3-dependent and AKT-independent manner. The expression of INPP4B partially upregulated by NPM1-mA is due to ERK/Ets-1 signaling

Techniques Used: Expressing, Mutagenesis, Functional Assay

High expression levels of INPP4B in leukemia cells with the NPM1 mutation. a RNA-seq mRNA expression data from the TCGA database were used to compare INPP4B expression between AML patients with ( n = 41) and without NPM1 mutation ( n = 130). * p
Figure Legend Snippet: High expression levels of INPP4B in leukemia cells with the NPM1 mutation. a RNA-seq mRNA expression data from the TCGA database were used to compare INPP4B expression between AML patients with ( n = 41) and without NPM1 mutation ( n = 130). * p

Techniques Used: Expressing, Mutagenesis, RNA Sequencing Assay

16) Product Images from "INPP4B promotes cell survival via SGK3 activation in NPM1-mutated leukemia"

Article Title: INPP4B promotes cell survival via SGK3 activation in NPM1-mutated leukemia

Journal: Journal of Experimental & Clinical Cancer Research : CR

doi: 10.1186/s13046-018-0675-9

INPP4B is upregulated by NPM1-mA in leukemia cells via ERK/Ets-1 signaling. ( a ) qRT-PCR analysis of INPP4B mRNA expression, ( b ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. ( c ) qRT-PCR analysis of INPP4B mRNA expression, ( d ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the THP-1 and K562 cells transduced with the plasmids expressing NPM1-wt or NPM1-mA. ( e ) qRT-PCR analysis of INPP4B mRNA expression, ( f ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3, p-Ets-1 and Ets-1 from the OCI-AML3 cells transfected with the control siRNA or siEts-1. ( g ) Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 and SGK3, ( h ) qRT-PCR analysis of INPP4B mRNA expression from the OCI-AML3 cells treated with different concentration of PD98059 (0, 10, 20 and 40 μM). i Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. Proteins were quantified using image software and normalized against β-actin. Data were represented as mean ± s.d. of three individual experiments. * p
Figure Legend Snippet: INPP4B is upregulated by NPM1-mA in leukemia cells via ERK/Ets-1 signaling. ( a ) qRT-PCR analysis of INPP4B mRNA expression, ( b ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. ( c ) qRT-PCR analysis of INPP4B mRNA expression, ( d ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the THP-1 and K562 cells transduced with the plasmids expressing NPM1-wt or NPM1-mA. ( e ) qRT-PCR analysis of INPP4B mRNA expression, ( f ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3, p-Ets-1 and Ets-1 from the OCI-AML3 cells transfected with the control siRNA or siEts-1. ( g ) Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 and SGK3, ( h ) qRT-PCR analysis of INPP4B mRNA expression from the OCI-AML3 cells treated with different concentration of PD98059 (0, 10, 20 and 40 μM). i Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. Proteins were quantified using image software and normalized against β-actin. Data were represented as mean ± s.d. of three individual experiments. * p

Techniques Used: Quantitative RT-PCR, Expressing, Western Blot, Transduction, Transfection, Concentration Assay, Software

NPM1-mA-mediated INPP4B upregulation promotes cell proliferation in OCI-AML3 cells. The NPM1-mA-silenced OCI-AML3 cells were subjected to ( a ) CCK8 assays and ( b ) colony forming assays. c The NPM1-mA-silenced OCI-AML3 cells were transfected with the pEAK-Flag/INPP4B plasmids, western blotting analysis of INPP4B and NPM1-mA. Proteins were quantified using image software and normalized against β-actin. d CCK-8 assay analysis of cell proliferation in NPM1-mA-silenced OCI-AML3 cells, followed by Flag-INPP4B introduction. Data were represented as mean ± s.d. of three individual experiments. * p
Figure Legend Snippet: NPM1-mA-mediated INPP4B upregulation promotes cell proliferation in OCI-AML3 cells. The NPM1-mA-silenced OCI-AML3 cells were subjected to ( a ) CCK8 assays and ( b ) colony forming assays. c The NPM1-mA-silenced OCI-AML3 cells were transfected with the pEAK-Flag/INPP4B plasmids, western blotting analysis of INPP4B and NPM1-mA. Proteins were quantified using image software and normalized against β-actin. d CCK-8 assay analysis of cell proliferation in NPM1-mA-silenced OCI-AML3 cells, followed by Flag-INPP4B introduction. Data were represented as mean ± s.d. of three individual experiments. * p

Techniques Used: Transfection, Western Blot, Software, CCK-8 Assay

High expression of INPP4B is associated with poor survival outcome in NPM1-mutated leukemia. Kaplan-Meier survival data of 153 AML patients were used to analysis ( a ) OS and ( b ) EFS curves according to INPP4B levels. c Heatmap of 38 primary AML samples with NPM1 mutation from TCGA dataset in which INPP4B expression was aligned with patient event (Survival or Death). Kaplan-Meier survival data of patients with NPM1-mutated AML were used to analysis ( d ) OS and ( e ) EFS curves according to INPP4B levels. f Schematic diagram describing the functional significance of INPP4B in the NPM1-mutated leukemia cells. INPP4B promotes leukemia cell survival in a SGK3-dependent and AKT-independent manner. The expression of INPP4B partially upregulated by NPM1-mA is due to ERK/Ets-1 signaling
Figure Legend Snippet: High expression of INPP4B is associated with poor survival outcome in NPM1-mutated leukemia. Kaplan-Meier survival data of 153 AML patients were used to analysis ( a ) OS and ( b ) EFS curves according to INPP4B levels. c Heatmap of 38 primary AML samples with NPM1 mutation from TCGA dataset in which INPP4B expression was aligned with patient event (Survival or Death). Kaplan-Meier survival data of patients with NPM1-mutated AML were used to analysis ( d ) OS and ( e ) EFS curves according to INPP4B levels. f Schematic diagram describing the functional significance of INPP4B in the NPM1-mutated leukemia cells. INPP4B promotes leukemia cell survival in a SGK3-dependent and AKT-independent manner. The expression of INPP4B partially upregulated by NPM1-mA is due to ERK/Ets-1 signaling

Techniques Used: Expressing, Mutagenesis, Functional Assay

High expression levels of INPP4B in leukemia cells with the NPM1 mutation. a RNA-seq mRNA expression data from the TCGA database were used to compare INPP4B expression between AML patients with ( n = 41) and without NPM1 mutation ( n = 130). * p
Figure Legend Snippet: High expression levels of INPP4B in leukemia cells with the NPM1 mutation. a RNA-seq mRNA expression data from the TCGA database were used to compare INPP4B expression between AML patients with ( n = 41) and without NPM1 mutation ( n = 130). * p

Techniques Used: Expressing, Mutagenesis, RNA Sequencing Assay

17) Product Images from "INPP4B promotes cell survival via SGK3 activation in NPM1-mutated leukemia"

Article Title: INPP4B promotes cell survival via SGK3 activation in NPM1-mutated leukemia

Journal: Journal of Experimental & Clinical Cancer Research : CR

doi: 10.1186/s13046-018-0675-9

INPP4B is upregulated by NPM1-mA in leukemia cells via ERK/Ets-1 signaling. ( a ) qRT-PCR analysis of INPP4B mRNA expression, ( b ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. ( c ) qRT-PCR analysis of INPP4B mRNA expression, ( d ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the THP-1 and K562 cells transduced with the plasmids expressing NPM1-wt or NPM1-mA. ( e ) qRT-PCR analysis of INPP4B mRNA expression, ( f ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3, p-Ets-1 and Ets-1 from the OCI-AML3 cells transfected with the control siRNA or siEts-1. ( g ) Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 and SGK3, ( h ) qRT-PCR analysis of INPP4B mRNA expression from the OCI-AML3 cells treated with different concentration of PD98059 (0, 10, 20 and 40 μM). i Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. Proteins were quantified using image software and normalized against β-actin. Data were represented as mean ± s.d. of three individual experiments. * p
Figure Legend Snippet: INPP4B is upregulated by NPM1-mA in leukemia cells via ERK/Ets-1 signaling. ( a ) qRT-PCR analysis of INPP4B mRNA expression, ( b ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. ( c ) qRT-PCR analysis of INPP4B mRNA expression, ( d ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the THP-1 and K562 cells transduced with the plasmids expressing NPM1-wt or NPM1-mA. ( e ) qRT-PCR analysis of INPP4B mRNA expression, ( f ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3, p-Ets-1 and Ets-1 from the OCI-AML3 cells transfected with the control siRNA or siEts-1. ( g ) Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 and SGK3, ( h ) qRT-PCR analysis of INPP4B mRNA expression from the OCI-AML3 cells treated with different concentration of PD98059 (0, 10, 20 and 40 μM). i Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. Proteins were quantified using image software and normalized against β-actin. Data were represented as mean ± s.d. of three individual experiments. * p

Techniques Used: Quantitative RT-PCR, Expressing, Western Blot, Transduction, Transfection, Concentration Assay, Software

NPM1-mA-mediated INPP4B upregulation promotes cell proliferation in OCI-AML3 cells. The NPM1-mA-silenced OCI-AML3 cells were subjected to ( a ) CCK8 assays and ( b ) colony forming assays. c The NPM1-mA-silenced OCI-AML3 cells were transfected with the pEAK-Flag/INPP4B plasmids, western blotting analysis of INPP4B and NPM1-mA. Proteins were quantified using image software and normalized against β-actin. d CCK-8 assay analysis of cell proliferation in NPM1-mA-silenced OCI-AML3 cells, followed by Flag-INPP4B introduction. Data were represented as mean ± s.d. of three individual experiments. * p
Figure Legend Snippet: NPM1-mA-mediated INPP4B upregulation promotes cell proliferation in OCI-AML3 cells. The NPM1-mA-silenced OCI-AML3 cells were subjected to ( a ) CCK8 assays and ( b ) colony forming assays. c The NPM1-mA-silenced OCI-AML3 cells were transfected with the pEAK-Flag/INPP4B plasmids, western blotting analysis of INPP4B and NPM1-mA. Proteins were quantified using image software and normalized against β-actin. d CCK-8 assay analysis of cell proliferation in NPM1-mA-silenced OCI-AML3 cells, followed by Flag-INPP4B introduction. Data were represented as mean ± s.d. of three individual experiments. * p

Techniques Used: Transfection, Western Blot, Software, CCK-8 Assay

High expression of INPP4B is associated with poor survival outcome in NPM1-mutated leukemia. Kaplan-Meier survival data of 153 AML patients were used to analysis ( a ) OS and ( b ) EFS curves according to INPP4B levels. c Heatmap of 38 primary AML samples with NPM1 mutation from TCGA dataset in which INPP4B expression was aligned with patient event (Survival or Death). Kaplan-Meier survival data of patients with NPM1-mutated AML were used to analysis ( d ) OS and ( e ) EFS curves according to INPP4B levels. f Schematic diagram describing the functional significance of INPP4B in the NPM1-mutated leukemia cells. INPP4B promotes leukemia cell survival in a SGK3-dependent and AKT-independent manner. The expression of INPP4B partially upregulated by NPM1-mA is due to ERK/Ets-1 signaling
Figure Legend Snippet: High expression of INPP4B is associated with poor survival outcome in NPM1-mutated leukemia. Kaplan-Meier survival data of 153 AML patients were used to analysis ( a ) OS and ( b ) EFS curves according to INPP4B levels. c Heatmap of 38 primary AML samples with NPM1 mutation from TCGA dataset in which INPP4B expression was aligned with patient event (Survival or Death). Kaplan-Meier survival data of patients with NPM1-mutated AML were used to analysis ( d ) OS and ( e ) EFS curves according to INPP4B levels. f Schematic diagram describing the functional significance of INPP4B in the NPM1-mutated leukemia cells. INPP4B promotes leukemia cell survival in a SGK3-dependent and AKT-independent manner. The expression of INPP4B partially upregulated by NPM1-mA is due to ERK/Ets-1 signaling

Techniques Used: Expressing, Mutagenesis, Functional Assay

High expression levels of INPP4B in leukemia cells with the NPM1 mutation. a RNA-seq mRNA expression data from the TCGA database were used to compare INPP4B expression between AML patients with ( n = 41) and without NPM1 mutation ( n = 130). * p
Figure Legend Snippet: High expression levels of INPP4B in leukemia cells with the NPM1 mutation. a RNA-seq mRNA expression data from the TCGA database were used to compare INPP4B expression between AML patients with ( n = 41) and without NPM1 mutation ( n = 130). * p

Techniques Used: Expressing, Mutagenesis, RNA Sequencing Assay

18) Product Images from "INPP4B promotes cell survival via SGK3 activation in NPM1-mutated leukemia"

Article Title: INPP4B promotes cell survival via SGK3 activation in NPM1-mutated leukemia

Journal: Journal of Experimental & Clinical Cancer Research : CR

doi: 10.1186/s13046-018-0675-9

INPP4B is upregulated by NPM1-mA in leukemia cells via ERK/Ets-1 signaling. ( a ) qRT-PCR analysis of INPP4B mRNA expression, ( b ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. ( c ) qRT-PCR analysis of INPP4B mRNA expression, ( d ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the THP-1 and K562 cells transduced with the plasmids expressing NPM1-wt or NPM1-mA. ( e ) qRT-PCR analysis of INPP4B mRNA expression, ( f ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3, p-Ets-1 and Ets-1 from the OCI-AML3 cells transfected with the control siRNA or siEts-1. ( g ) Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 and SGK3, ( h ) qRT-PCR analysis of INPP4B mRNA expression from the OCI-AML3 cells treated with different concentration of PD98059 (0, 10, 20 and 40 μM). i Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. Proteins were quantified using image software and normalized against β-actin. Data were represented as mean ± s.d. of three individual experiments. * p
Figure Legend Snippet: INPP4B is upregulated by NPM1-mA in leukemia cells via ERK/Ets-1 signaling. ( a ) qRT-PCR analysis of INPP4B mRNA expression, ( b ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. ( c ) qRT-PCR analysis of INPP4B mRNA expression, ( d ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the THP-1 and K562 cells transduced with the plasmids expressing NPM1-wt or NPM1-mA. ( e ) qRT-PCR analysis of INPP4B mRNA expression, ( f ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3, p-Ets-1 and Ets-1 from the OCI-AML3 cells transfected with the control siRNA or siEts-1. ( g ) Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 and SGK3, ( h ) qRT-PCR analysis of INPP4B mRNA expression from the OCI-AML3 cells treated with different concentration of PD98059 (0, 10, 20 and 40 μM). i Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. Proteins were quantified using image software and normalized against β-actin. Data were represented as mean ± s.d. of three individual experiments. * p

Techniques Used: Quantitative RT-PCR, Expressing, Western Blot, Transduction, Transfection, Concentration Assay, Software

NPM1-mA-mediated INPP4B upregulation promotes cell proliferation in OCI-AML3 cells. The NPM1-mA-silenced OCI-AML3 cells were subjected to ( a ) CCK8 assays and ( b ) colony forming assays. c The NPM1-mA-silenced OCI-AML3 cells were transfected with the pEAK-Flag/INPP4B plasmids, western blotting analysis of INPP4B and NPM1-mA. Proteins were quantified using image software and normalized against β-actin. d CCK-8 assay analysis of cell proliferation in NPM1-mA-silenced OCI-AML3 cells, followed by Flag-INPP4B introduction. Data were represented as mean ± s.d. of three individual experiments. * p
Figure Legend Snippet: NPM1-mA-mediated INPP4B upregulation promotes cell proliferation in OCI-AML3 cells. The NPM1-mA-silenced OCI-AML3 cells were subjected to ( a ) CCK8 assays and ( b ) colony forming assays. c The NPM1-mA-silenced OCI-AML3 cells were transfected with the pEAK-Flag/INPP4B plasmids, western blotting analysis of INPP4B and NPM1-mA. Proteins were quantified using image software and normalized against β-actin. d CCK-8 assay analysis of cell proliferation in NPM1-mA-silenced OCI-AML3 cells, followed by Flag-INPP4B introduction. Data were represented as mean ± s.d. of three individual experiments. * p

Techniques Used: Transfection, Western Blot, Software, CCK-8 Assay

High expression of INPP4B is associated with poor survival outcome in NPM1-mutated leukemia. Kaplan-Meier survival data of 153 AML patients were used to analysis ( a ) OS and ( b ) EFS curves according to INPP4B levels. c Heatmap of 38 primary AML samples with NPM1 mutation from TCGA dataset in which INPP4B expression was aligned with patient event (Survival or Death). Kaplan-Meier survival data of patients with NPM1-mutated AML were used to analysis ( d ) OS and ( e ) EFS curves according to INPP4B levels. f Schematic diagram describing the functional significance of INPP4B in the NPM1-mutated leukemia cells. INPP4B promotes leukemia cell survival in a SGK3-dependent and AKT-independent manner. The expression of INPP4B partially upregulated by NPM1-mA is due to ERK/Ets-1 signaling
Figure Legend Snippet: High expression of INPP4B is associated with poor survival outcome in NPM1-mutated leukemia. Kaplan-Meier survival data of 153 AML patients were used to analysis ( a ) OS and ( b ) EFS curves according to INPP4B levels. c Heatmap of 38 primary AML samples with NPM1 mutation from TCGA dataset in which INPP4B expression was aligned with patient event (Survival or Death). Kaplan-Meier survival data of patients with NPM1-mutated AML were used to analysis ( d ) OS and ( e ) EFS curves according to INPP4B levels. f Schematic diagram describing the functional significance of INPP4B in the NPM1-mutated leukemia cells. INPP4B promotes leukemia cell survival in a SGK3-dependent and AKT-independent manner. The expression of INPP4B partially upregulated by NPM1-mA is due to ERK/Ets-1 signaling

Techniques Used: Expressing, Mutagenesis, Functional Assay

High expression levels of INPP4B in leukemia cells with the NPM1 mutation. a RNA-seq mRNA expression data from the TCGA database were used to compare INPP4B expression between AML patients with ( n = 41) and without NPM1 mutation ( n = 130). * p
Figure Legend Snippet: High expression levels of INPP4B in leukemia cells with the NPM1 mutation. a RNA-seq mRNA expression data from the TCGA database were used to compare INPP4B expression between AML patients with ( n = 41) and without NPM1 mutation ( n = 130). * p

Techniques Used: Expressing, Mutagenesis, RNA Sequencing Assay

19) Product Images from "INPP4B promotes cell survival via SGK3 activation in NPM1-mutated leukemia"

Article Title: INPP4B promotes cell survival via SGK3 activation in NPM1-mutated leukemia

Journal: Journal of Experimental & Clinical Cancer Research : CR

doi: 10.1186/s13046-018-0675-9

INPP4B is upregulated by NPM1-mA in leukemia cells via ERK/Ets-1 signaling. ( a ) qRT-PCR analysis of INPP4B mRNA expression, ( b ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. ( c ) qRT-PCR analysis of INPP4B mRNA expression, ( d ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the THP-1 and K562 cells transduced with the plasmids expressing NPM1-wt or NPM1-mA. ( e ) qRT-PCR analysis of INPP4B mRNA expression, ( f ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3, p-Ets-1 and Ets-1 from the OCI-AML3 cells transfected with the control siRNA or siEts-1. ( g ) Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 and SGK3, ( h ) qRT-PCR analysis of INPP4B mRNA expression from the OCI-AML3 cells treated with different concentration of PD98059 (0, 10, 20 and 40 μM). i Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. Proteins were quantified using image software and normalized against β-actin. Data were represented as mean ± s.d. of three individual experiments. * p
Figure Legend Snippet: INPP4B is upregulated by NPM1-mA in leukemia cells via ERK/Ets-1 signaling. ( a ) qRT-PCR analysis of INPP4B mRNA expression, ( b ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. ( c ) qRT-PCR analysis of INPP4B mRNA expression, ( d ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the THP-1 and K562 cells transduced with the plasmids expressing NPM1-wt or NPM1-mA. ( e ) qRT-PCR analysis of INPP4B mRNA expression, ( f ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3, p-Ets-1 and Ets-1 from the OCI-AML3 cells transfected with the control siRNA or siEts-1. ( g ) Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 and SGK3, ( h ) qRT-PCR analysis of INPP4B mRNA expression from the OCI-AML3 cells treated with different concentration of PD98059 (0, 10, 20 and 40 μM). i Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. Proteins were quantified using image software and normalized against β-actin. Data were represented as mean ± s.d. of three individual experiments. * p

Techniques Used: Quantitative RT-PCR, Expressing, Western Blot, Transduction, Transfection, Concentration Assay, Software

NPM1-mA-mediated INPP4B upregulation promotes cell proliferation in OCI-AML3 cells. The NPM1-mA-silenced OCI-AML3 cells were subjected to ( a ) CCK8 assays and ( b ) colony forming assays. c The NPM1-mA-silenced OCI-AML3 cells were transfected with the pEAK-Flag/INPP4B plasmids, western blotting analysis of INPP4B and NPM1-mA. Proteins were quantified using image software and normalized against β-actin. d CCK-8 assay analysis of cell proliferation in NPM1-mA-silenced OCI-AML3 cells, followed by Flag-INPP4B introduction. Data were represented as mean ± s.d. of three individual experiments. * p
Figure Legend Snippet: NPM1-mA-mediated INPP4B upregulation promotes cell proliferation in OCI-AML3 cells. The NPM1-mA-silenced OCI-AML3 cells were subjected to ( a ) CCK8 assays and ( b ) colony forming assays. c The NPM1-mA-silenced OCI-AML3 cells were transfected with the pEAK-Flag/INPP4B plasmids, western blotting analysis of INPP4B and NPM1-mA. Proteins were quantified using image software and normalized against β-actin. d CCK-8 assay analysis of cell proliferation in NPM1-mA-silenced OCI-AML3 cells, followed by Flag-INPP4B introduction. Data were represented as mean ± s.d. of three individual experiments. * p

Techniques Used: Transfection, Western Blot, Software, CCK-8 Assay

High expression of INPP4B is associated with poor survival outcome in NPM1-mutated leukemia. Kaplan-Meier survival data of 153 AML patients were used to analysis ( a ) OS and ( b ) EFS curves according to INPP4B levels. c Heatmap of 38 primary AML samples with NPM1 mutation from TCGA dataset in which INPP4B expression was aligned with patient event (Survival or Death). Kaplan-Meier survival data of patients with NPM1-mutated AML were used to analysis ( d ) OS and ( e ) EFS curves according to INPP4B levels. f Schematic diagram describing the functional significance of INPP4B in the NPM1-mutated leukemia cells. INPP4B promotes leukemia cell survival in a SGK3-dependent and AKT-independent manner. The expression of INPP4B partially upregulated by NPM1-mA is due to ERK/Ets-1 signaling
Figure Legend Snippet: High expression of INPP4B is associated with poor survival outcome in NPM1-mutated leukemia. Kaplan-Meier survival data of 153 AML patients were used to analysis ( a ) OS and ( b ) EFS curves according to INPP4B levels. c Heatmap of 38 primary AML samples with NPM1 mutation from TCGA dataset in which INPP4B expression was aligned with patient event (Survival or Death). Kaplan-Meier survival data of patients with NPM1-mutated AML were used to analysis ( d ) OS and ( e ) EFS curves according to INPP4B levels. f Schematic diagram describing the functional significance of INPP4B in the NPM1-mutated leukemia cells. INPP4B promotes leukemia cell survival in a SGK3-dependent and AKT-independent manner. The expression of INPP4B partially upregulated by NPM1-mA is due to ERK/Ets-1 signaling

Techniques Used: Expressing, Mutagenesis, Functional Assay

High expression levels of INPP4B in leukemia cells with the NPM1 mutation. a RNA-seq mRNA expression data from the TCGA database were used to compare INPP4B expression between AML patients with ( n = 41) and without NPM1 mutation ( n = 130). * p
Figure Legend Snippet: High expression levels of INPP4B in leukemia cells with the NPM1 mutation. a RNA-seq mRNA expression data from the TCGA database were used to compare INPP4B expression between AML patients with ( n = 41) and without NPM1 mutation ( n = 130). * p

Techniques Used: Expressing, Mutagenesis, RNA Sequencing Assay

20) Product Images from "INPP4B promotes cell survival via SGK3 activation in NPM1-mutated leukemia"

Article Title: INPP4B promotes cell survival via SGK3 activation in NPM1-mutated leukemia

Journal: Journal of Experimental & Clinical Cancer Research : CR

doi: 10.1186/s13046-018-0675-9

INPP4B is upregulated by NPM1-mA in leukemia cells via ERK/Ets-1 signaling. ( a ) qRT-PCR analysis of INPP4B mRNA expression, ( b ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. ( c ) qRT-PCR analysis of INPP4B mRNA expression, ( d ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the THP-1 and K562 cells transduced with the plasmids expressing NPM1-wt or NPM1-mA. ( e ) qRT-PCR analysis of INPP4B mRNA expression, ( f ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3, p-Ets-1 and Ets-1 from the OCI-AML3 cells transfected with the control siRNA or siEts-1. ( g ) Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 and SGK3, ( h ) qRT-PCR analysis of INPP4B mRNA expression from the OCI-AML3 cells treated with different concentration of PD98059 (0, 10, 20 and 40 μM). i Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. Proteins were quantified using image software and normalized against β-actin. Data were represented as mean ± s.d. of three individual experiments. * p
Figure Legend Snippet: INPP4B is upregulated by NPM1-mA in leukemia cells via ERK/Ets-1 signaling. ( a ) qRT-PCR analysis of INPP4B mRNA expression, ( b ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. ( c ) qRT-PCR analysis of INPP4B mRNA expression, ( d ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the THP-1 and K562 cells transduced with the plasmids expressing NPM1-wt or NPM1-mA. ( e ) qRT-PCR analysis of INPP4B mRNA expression, ( f ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3, p-Ets-1 and Ets-1 from the OCI-AML3 cells transfected with the control siRNA or siEts-1. ( g ) Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 and SGK3, ( h ) qRT-PCR analysis of INPP4B mRNA expression from the OCI-AML3 cells treated with different concentration of PD98059 (0, 10, 20 and 40 μM). i Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. Proteins were quantified using image software and normalized against β-actin. Data were represented as mean ± s.d. of three individual experiments. * p

Techniques Used: Quantitative RT-PCR, Expressing, Western Blot, Transduction, Transfection, Concentration Assay, Software

NPM1-mA-mediated INPP4B upregulation promotes cell proliferation in OCI-AML3 cells. The NPM1-mA-silenced OCI-AML3 cells were subjected to ( a ) CCK8 assays and ( b ) colony forming assays. c The NPM1-mA-silenced OCI-AML3 cells were transfected with the pEAK-Flag/INPP4B plasmids, western blotting analysis of INPP4B and NPM1-mA. Proteins were quantified using image software and normalized against β-actin. d CCK-8 assay analysis of cell proliferation in NPM1-mA-silenced OCI-AML3 cells, followed by Flag-INPP4B introduction. Data were represented as mean ± s.d. of three individual experiments. * p
Figure Legend Snippet: NPM1-mA-mediated INPP4B upregulation promotes cell proliferation in OCI-AML3 cells. The NPM1-mA-silenced OCI-AML3 cells were subjected to ( a ) CCK8 assays and ( b ) colony forming assays. c The NPM1-mA-silenced OCI-AML3 cells were transfected with the pEAK-Flag/INPP4B plasmids, western blotting analysis of INPP4B and NPM1-mA. Proteins were quantified using image software and normalized against β-actin. d CCK-8 assay analysis of cell proliferation in NPM1-mA-silenced OCI-AML3 cells, followed by Flag-INPP4B introduction. Data were represented as mean ± s.d. of three individual experiments. * p

Techniques Used: Transfection, Western Blot, Software, CCK-8 Assay

High expression of INPP4B is associated with poor survival outcome in NPM1-mutated leukemia. Kaplan-Meier survival data of 153 AML patients were used to analysis ( a ) OS and ( b ) EFS curves according to INPP4B levels. c Heatmap of 38 primary AML samples with NPM1 mutation from TCGA dataset in which INPP4B expression was aligned with patient event (Survival or Death). Kaplan-Meier survival data of patients with NPM1-mutated AML were used to analysis ( d ) OS and ( e ) EFS curves according to INPP4B levels. f Schematic diagram describing the functional significance of INPP4B in the NPM1-mutated leukemia cells. INPP4B promotes leukemia cell survival in a SGK3-dependent and AKT-independent manner. The expression of INPP4B partially upregulated by NPM1-mA is due to ERK/Ets-1 signaling
Figure Legend Snippet: High expression of INPP4B is associated with poor survival outcome in NPM1-mutated leukemia. Kaplan-Meier survival data of 153 AML patients were used to analysis ( a ) OS and ( b ) EFS curves according to INPP4B levels. c Heatmap of 38 primary AML samples with NPM1 mutation from TCGA dataset in which INPP4B expression was aligned with patient event (Survival or Death). Kaplan-Meier survival data of patients with NPM1-mutated AML were used to analysis ( d ) OS and ( e ) EFS curves according to INPP4B levels. f Schematic diagram describing the functional significance of INPP4B in the NPM1-mutated leukemia cells. INPP4B promotes leukemia cell survival in a SGK3-dependent and AKT-independent manner. The expression of INPP4B partially upregulated by NPM1-mA is due to ERK/Ets-1 signaling

Techniques Used: Expressing, Mutagenesis, Functional Assay

High expression levels of INPP4B in leukemia cells with the NPM1 mutation. a RNA-seq mRNA expression data from the TCGA database were used to compare INPP4B expression between AML patients with ( n = 41) and without NPM1 mutation ( n = 130). * p
Figure Legend Snippet: High expression levels of INPP4B in leukemia cells with the NPM1 mutation. a RNA-seq mRNA expression data from the TCGA database were used to compare INPP4B expression between AML patients with ( n = 41) and without NPM1 mutation ( n = 130). * p

Techniques Used: Expressing, Mutagenesis, RNA Sequencing Assay

21) Product Images from "INPP4B promotes cell survival via SGK3 activation in NPM1-mutated leukemia"

Article Title: INPP4B promotes cell survival via SGK3 activation in NPM1-mutated leukemia

Journal: Journal of Experimental & Clinical Cancer Research : CR

doi: 10.1186/s13046-018-0675-9

INPP4B is upregulated by NPM1-mA in leukemia cells via ERK/Ets-1 signaling. ( a ) qRT-PCR analysis of INPP4B mRNA expression, ( b ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. ( c ) qRT-PCR analysis of INPP4B mRNA expression, ( d ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the THP-1 and K562 cells transduced with the plasmids expressing NPM1-wt or NPM1-mA. ( e ) qRT-PCR analysis of INPP4B mRNA expression, ( f ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3, p-Ets-1 and Ets-1 from the OCI-AML3 cells transfected with the control siRNA or siEts-1. ( g ) Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 and SGK3, ( h ) qRT-PCR analysis of INPP4B mRNA expression from the OCI-AML3 cells treated with different concentration of PD98059 (0, 10, 20 and 40 μM). i Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. Proteins were quantified using image software and normalized against β-actin. Data were represented as mean ± s.d. of three individual experiments. * p
Figure Legend Snippet: INPP4B is upregulated by NPM1-mA in leukemia cells via ERK/Ets-1 signaling. ( a ) qRT-PCR analysis of INPP4B mRNA expression, ( b ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. ( c ) qRT-PCR analysis of INPP4B mRNA expression, ( d ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the THP-1 and K562 cells transduced with the plasmids expressing NPM1-wt or NPM1-mA. ( e ) qRT-PCR analysis of INPP4B mRNA expression, ( f ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3, p-Ets-1 and Ets-1 from the OCI-AML3 cells transfected with the control siRNA or siEts-1. ( g ) Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 and SGK3, ( h ) qRT-PCR analysis of INPP4B mRNA expression from the OCI-AML3 cells treated with different concentration of PD98059 (0, 10, 20 and 40 μM). i Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. Proteins were quantified using image software and normalized against β-actin. Data were represented as mean ± s.d. of three individual experiments. * p

Techniques Used: Quantitative RT-PCR, Expressing, Western Blot, Transduction, Transfection, Concentration Assay, Software

NPM1-mA-mediated INPP4B upregulation promotes cell proliferation in OCI-AML3 cells. The NPM1-mA-silenced OCI-AML3 cells were subjected to ( a ) CCK8 assays and ( b ) colony forming assays. c The NPM1-mA-silenced OCI-AML3 cells were transfected with the pEAK-Flag/INPP4B plasmids, western blotting analysis of INPP4B and NPM1-mA. Proteins were quantified using image software and normalized against β-actin. d CCK-8 assay analysis of cell proliferation in NPM1-mA-silenced OCI-AML3 cells, followed by Flag-INPP4B introduction. Data were represented as mean ± s.d. of three individual experiments. * p
Figure Legend Snippet: NPM1-mA-mediated INPP4B upregulation promotes cell proliferation in OCI-AML3 cells. The NPM1-mA-silenced OCI-AML3 cells were subjected to ( a ) CCK8 assays and ( b ) colony forming assays. c The NPM1-mA-silenced OCI-AML3 cells were transfected with the pEAK-Flag/INPP4B plasmids, western blotting analysis of INPP4B and NPM1-mA. Proteins were quantified using image software and normalized against β-actin. d CCK-8 assay analysis of cell proliferation in NPM1-mA-silenced OCI-AML3 cells, followed by Flag-INPP4B introduction. Data were represented as mean ± s.d. of three individual experiments. * p

Techniques Used: Transfection, Western Blot, Software, CCK-8 Assay

High expression of INPP4B is associated with poor survival outcome in NPM1-mutated leukemia. Kaplan-Meier survival data of 153 AML patients were used to analysis ( a ) OS and ( b ) EFS curves according to INPP4B levels. c Heatmap of 38 primary AML samples with NPM1 mutation from TCGA dataset in which INPP4B expression was aligned with patient event (Survival or Death). Kaplan-Meier survival data of patients with NPM1-mutated AML were used to analysis ( d ) OS and ( e ) EFS curves according to INPP4B levels. f Schematic diagram describing the functional significance of INPP4B in the NPM1-mutated leukemia cells. INPP4B promotes leukemia cell survival in a SGK3-dependent and AKT-independent manner. The expression of INPP4B partially upregulated by NPM1-mA is due to ERK/Ets-1 signaling
Figure Legend Snippet: High expression of INPP4B is associated with poor survival outcome in NPM1-mutated leukemia. Kaplan-Meier survival data of 153 AML patients were used to analysis ( a ) OS and ( b ) EFS curves according to INPP4B levels. c Heatmap of 38 primary AML samples with NPM1 mutation from TCGA dataset in which INPP4B expression was aligned with patient event (Survival or Death). Kaplan-Meier survival data of patients with NPM1-mutated AML were used to analysis ( d ) OS and ( e ) EFS curves according to INPP4B levels. f Schematic diagram describing the functional significance of INPP4B in the NPM1-mutated leukemia cells. INPP4B promotes leukemia cell survival in a SGK3-dependent and AKT-independent manner. The expression of INPP4B partially upregulated by NPM1-mA is due to ERK/Ets-1 signaling

Techniques Used: Expressing, Mutagenesis, Functional Assay

High expression levels of INPP4B in leukemia cells with the NPM1 mutation. a RNA-seq mRNA expression data from the TCGA database were used to compare INPP4B expression between AML patients with ( n = 41) and without NPM1 mutation ( n = 130). * p
Figure Legend Snippet: High expression levels of INPP4B in leukemia cells with the NPM1 mutation. a RNA-seq mRNA expression data from the TCGA database were used to compare INPP4B expression between AML patients with ( n = 41) and without NPM1 mutation ( n = 130). * p

Techniques Used: Expressing, Mutagenesis, RNA Sequencing Assay

22) Product Images from "INPP4B promotes cell survival via SGK3 activation in NPM1-mutated leukemia"

Article Title: INPP4B promotes cell survival via SGK3 activation in NPM1-mutated leukemia

Journal: Journal of Experimental & Clinical Cancer Research : CR

doi: 10.1186/s13046-018-0675-9

INPP4B is upregulated by NPM1-mA in leukemia cells via ERK/Ets-1 signaling. ( a ) qRT-PCR analysis of INPP4B mRNA expression, ( b ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. ( c ) qRT-PCR analysis of INPP4B mRNA expression, ( d ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the THP-1 and K562 cells transduced with the plasmids expressing NPM1-wt or NPM1-mA. ( e ) qRT-PCR analysis of INPP4B mRNA expression, ( f ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3, p-Ets-1 and Ets-1 from the OCI-AML3 cells transfected with the control siRNA or siEts-1. ( g ) Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 and SGK3, ( h ) qRT-PCR analysis of INPP4B mRNA expression from the OCI-AML3 cells treated with different concentration of PD98059 (0, 10, 20 and 40 μM). i Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. Proteins were quantified using image software and normalized against β-actin. Data were represented as mean ± s.d. of three individual experiments. * p
Figure Legend Snippet: INPP4B is upregulated by NPM1-mA in leukemia cells via ERK/Ets-1 signaling. ( a ) qRT-PCR analysis of INPP4B mRNA expression, ( b ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. ( c ) qRT-PCR analysis of INPP4B mRNA expression, ( d ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the THP-1 and K562 cells transduced with the plasmids expressing NPM1-wt or NPM1-mA. ( e ) qRT-PCR analysis of INPP4B mRNA expression, ( f ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3, p-Ets-1 and Ets-1 from the OCI-AML3 cells transfected with the control siRNA or siEts-1. ( g ) Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 and SGK3, ( h ) qRT-PCR analysis of INPP4B mRNA expression from the OCI-AML3 cells treated with different concentration of PD98059 (0, 10, 20 and 40 μM). i Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. Proteins were quantified using image software and normalized against β-actin. Data were represented as mean ± s.d. of three individual experiments. * p

Techniques Used: Quantitative RT-PCR, Expressing, Western Blot, Transduction, Transfection, Concentration Assay, Software

NPM1-mA-mediated INPP4B upregulation promotes cell proliferation in OCI-AML3 cells. The NPM1-mA-silenced OCI-AML3 cells were subjected to ( a ) CCK8 assays and ( b ) colony forming assays. c The NPM1-mA-silenced OCI-AML3 cells were transfected with the pEAK-Flag/INPP4B plasmids, western blotting analysis of INPP4B and NPM1-mA. Proteins were quantified using image software and normalized against β-actin. d CCK-8 assay analysis of cell proliferation in NPM1-mA-silenced OCI-AML3 cells, followed by Flag-INPP4B introduction. Data were represented as mean ± s.d. of three individual experiments. * p
Figure Legend Snippet: NPM1-mA-mediated INPP4B upregulation promotes cell proliferation in OCI-AML3 cells. The NPM1-mA-silenced OCI-AML3 cells were subjected to ( a ) CCK8 assays and ( b ) colony forming assays. c The NPM1-mA-silenced OCI-AML3 cells were transfected with the pEAK-Flag/INPP4B plasmids, western blotting analysis of INPP4B and NPM1-mA. Proteins were quantified using image software and normalized against β-actin. d CCK-8 assay analysis of cell proliferation in NPM1-mA-silenced OCI-AML3 cells, followed by Flag-INPP4B introduction. Data were represented as mean ± s.d. of three individual experiments. * p

Techniques Used: Transfection, Western Blot, Software, CCK-8 Assay

High expression of INPP4B is associated with poor survival outcome in NPM1-mutated leukemia. Kaplan-Meier survival data of 153 AML patients were used to analysis ( a ) OS and ( b ) EFS curves according to INPP4B levels. c Heatmap of 38 primary AML samples with NPM1 mutation from TCGA dataset in which INPP4B expression was aligned with patient event (Survival or Death). Kaplan-Meier survival data of patients with NPM1-mutated AML were used to analysis ( d ) OS and ( e ) EFS curves according to INPP4B levels. f Schematic diagram describing the functional significance of INPP4B in the NPM1-mutated leukemia cells. INPP4B promotes leukemia cell survival in a SGK3-dependent and AKT-independent manner. The expression of INPP4B partially upregulated by NPM1-mA is due to ERK/Ets-1 signaling
Figure Legend Snippet: High expression of INPP4B is associated with poor survival outcome in NPM1-mutated leukemia. Kaplan-Meier survival data of 153 AML patients were used to analysis ( a ) OS and ( b ) EFS curves according to INPP4B levels. c Heatmap of 38 primary AML samples with NPM1 mutation from TCGA dataset in which INPP4B expression was aligned with patient event (Survival or Death). Kaplan-Meier survival data of patients with NPM1-mutated AML were used to analysis ( d ) OS and ( e ) EFS curves according to INPP4B levels. f Schematic diagram describing the functional significance of INPP4B in the NPM1-mutated leukemia cells. INPP4B promotes leukemia cell survival in a SGK3-dependent and AKT-independent manner. The expression of INPP4B partially upregulated by NPM1-mA is due to ERK/Ets-1 signaling

Techniques Used: Expressing, Mutagenesis, Functional Assay

High expression levels of INPP4B in leukemia cells with the NPM1 mutation. a RNA-seq mRNA expression data from the TCGA database were used to compare INPP4B expression between AML patients with ( n = 41) and without NPM1 mutation ( n = 130). * p
Figure Legend Snippet: High expression levels of INPP4B in leukemia cells with the NPM1 mutation. a RNA-seq mRNA expression data from the TCGA database were used to compare INPP4B expression between AML patients with ( n = 41) and without NPM1 mutation ( n = 130). * p

Techniques Used: Expressing, Mutagenesis, RNA Sequencing Assay

23) Product Images from "INPP4B promotes cell survival via SGK3 activation in NPM1-mutated leukemia"

Article Title: INPP4B promotes cell survival via SGK3 activation in NPM1-mutated leukemia

Journal: Journal of Experimental & Clinical Cancer Research : CR

doi: 10.1186/s13046-018-0675-9

INPP4B is upregulated by NPM1-mA in leukemia cells via ERK/Ets-1 signaling. ( a ) qRT-PCR analysis of INPP4B mRNA expression, ( b ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. ( c ) qRT-PCR analysis of INPP4B mRNA expression, ( d ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the THP-1 and K562 cells transduced with the plasmids expressing NPM1-wt or NPM1-mA. ( e ) qRT-PCR analysis of INPP4B mRNA expression, ( f ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3, p-Ets-1 and Ets-1 from the OCI-AML3 cells transfected with the control siRNA or siEts-1. ( g ) Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 and SGK3, ( h ) qRT-PCR analysis of INPP4B mRNA expression from the OCI-AML3 cells treated with different concentration of PD98059 (0, 10, 20 and 40 μM). i Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. Proteins were quantified using image software and normalized against β-actin. Data were represented as mean ± s.d. of three individual experiments. * p
Figure Legend Snippet: INPP4B is upregulated by NPM1-mA in leukemia cells via ERK/Ets-1 signaling. ( a ) qRT-PCR analysis of INPP4B mRNA expression, ( b ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. ( c ) qRT-PCR analysis of INPP4B mRNA expression, ( d ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the THP-1 and K562 cells transduced with the plasmids expressing NPM1-wt or NPM1-mA. ( e ) qRT-PCR analysis of INPP4B mRNA expression, ( f ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3, p-Ets-1 and Ets-1 from the OCI-AML3 cells transfected with the control siRNA or siEts-1. ( g ) Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 and SGK3, ( h ) qRT-PCR analysis of INPP4B mRNA expression from the OCI-AML3 cells treated with different concentration of PD98059 (0, 10, 20 and 40 μM). i Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. Proteins were quantified using image software and normalized against β-actin. Data were represented as mean ± s.d. of three individual experiments. * p

Techniques Used: Quantitative RT-PCR, Expressing, Western Blot, Transduction, Transfection, Concentration Assay, Software

NPM1-mA-mediated INPP4B upregulation promotes cell proliferation in OCI-AML3 cells. The NPM1-mA-silenced OCI-AML3 cells were subjected to ( a ) CCK8 assays and ( b ) colony forming assays. c The NPM1-mA-silenced OCI-AML3 cells were transfected with the pEAK-Flag/INPP4B plasmids, western blotting analysis of INPP4B and NPM1-mA. Proteins were quantified using image software and normalized against β-actin. d CCK-8 assay analysis of cell proliferation in NPM1-mA-silenced OCI-AML3 cells, followed by Flag-INPP4B introduction. Data were represented as mean ± s.d. of three individual experiments. * p
Figure Legend Snippet: NPM1-mA-mediated INPP4B upregulation promotes cell proliferation in OCI-AML3 cells. The NPM1-mA-silenced OCI-AML3 cells were subjected to ( a ) CCK8 assays and ( b ) colony forming assays. c The NPM1-mA-silenced OCI-AML3 cells were transfected with the pEAK-Flag/INPP4B plasmids, western blotting analysis of INPP4B and NPM1-mA. Proteins were quantified using image software and normalized against β-actin. d CCK-8 assay analysis of cell proliferation in NPM1-mA-silenced OCI-AML3 cells, followed by Flag-INPP4B introduction. Data were represented as mean ± s.d. of three individual experiments. * p

Techniques Used: Transfection, Western Blot, Software, CCK-8 Assay

High expression of INPP4B is associated with poor survival outcome in NPM1-mutated leukemia. Kaplan-Meier survival data of 153 AML patients were used to analysis ( a ) OS and ( b ) EFS curves according to INPP4B levels. c Heatmap of 38 primary AML samples with NPM1 mutation from TCGA dataset in which INPP4B expression was aligned with patient event (Survival or Death). Kaplan-Meier survival data of patients with NPM1-mutated AML were used to analysis ( d ) OS and ( e ) EFS curves according to INPP4B levels. f Schematic diagram describing the functional significance of INPP4B in the NPM1-mutated leukemia cells. INPP4B promotes leukemia cell survival in a SGK3-dependent and AKT-independent manner. The expression of INPP4B partially upregulated by NPM1-mA is due to ERK/Ets-1 signaling
Figure Legend Snippet: High expression of INPP4B is associated with poor survival outcome in NPM1-mutated leukemia. Kaplan-Meier survival data of 153 AML patients were used to analysis ( a ) OS and ( b ) EFS curves according to INPP4B levels. c Heatmap of 38 primary AML samples with NPM1 mutation from TCGA dataset in which INPP4B expression was aligned with patient event (Survival or Death). Kaplan-Meier survival data of patients with NPM1-mutated AML were used to analysis ( d ) OS and ( e ) EFS curves according to INPP4B levels. f Schematic diagram describing the functional significance of INPP4B in the NPM1-mutated leukemia cells. INPP4B promotes leukemia cell survival in a SGK3-dependent and AKT-independent manner. The expression of INPP4B partially upregulated by NPM1-mA is due to ERK/Ets-1 signaling

Techniques Used: Expressing, Mutagenesis, Functional Assay

High expression levels of INPP4B in leukemia cells with the NPM1 mutation. a RNA-seq mRNA expression data from the TCGA database were used to compare INPP4B expression between AML patients with ( n = 41) and without NPM1 mutation ( n = 130). * p
Figure Legend Snippet: High expression levels of INPP4B in leukemia cells with the NPM1 mutation. a RNA-seq mRNA expression data from the TCGA database were used to compare INPP4B expression between AML patients with ( n = 41) and without NPM1 mutation ( n = 130). * p

Techniques Used: Expressing, Mutagenesis, RNA Sequencing Assay

24) Product Images from "INPP4B promotes cell survival via SGK3 activation in NPM1-mutated leukemia"

Article Title: INPP4B promotes cell survival via SGK3 activation in NPM1-mutated leukemia

Journal: Journal of Experimental & Clinical Cancer Research : CR

doi: 10.1186/s13046-018-0675-9

INPP4B is upregulated by NPM1-mA in leukemia cells via ERK/Ets-1 signaling. ( a ) qRT-PCR analysis of INPP4B mRNA expression, ( b ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. ( c ) qRT-PCR analysis of INPP4B mRNA expression, ( d ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the THP-1 and K562 cells transduced with the plasmids expressing NPM1-wt or NPM1-mA. ( e ) qRT-PCR analysis of INPP4B mRNA expression, ( f ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3, p-Ets-1 and Ets-1 from the OCI-AML3 cells transfected with the control siRNA or siEts-1. ( g ) Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 and SGK3, ( h ) qRT-PCR analysis of INPP4B mRNA expression from the OCI-AML3 cells treated with different concentration of PD98059 (0, 10, 20 and 40 μM). i Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. Proteins were quantified using image software and normalized against β-actin. Data were represented as mean ± s.d. of three individual experiments. * p
Figure Legend Snippet: INPP4B is upregulated by NPM1-mA in leukemia cells via ERK/Ets-1 signaling. ( a ) qRT-PCR analysis of INPP4B mRNA expression, ( b ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. ( c ) qRT-PCR analysis of INPP4B mRNA expression, ( d ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the THP-1 and K562 cells transduced with the plasmids expressing NPM1-wt or NPM1-mA. ( e ) qRT-PCR analysis of INPP4B mRNA expression, ( f ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3, p-Ets-1 and Ets-1 from the OCI-AML3 cells transfected with the control siRNA or siEts-1. ( g ) Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 and SGK3, ( h ) qRT-PCR analysis of INPP4B mRNA expression from the OCI-AML3 cells treated with different concentration of PD98059 (0, 10, 20 and 40 μM). i Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. Proteins were quantified using image software and normalized against β-actin. Data were represented as mean ± s.d. of three individual experiments. * p

Techniques Used: Quantitative RT-PCR, Expressing, Western Blot, Transduction, Transfection, Concentration Assay, Software

NPM1-mA-mediated INPP4B upregulation promotes cell proliferation in OCI-AML3 cells. The NPM1-mA-silenced OCI-AML3 cells were subjected to ( a ) CCK8 assays and ( b ) colony forming assays. c The NPM1-mA-silenced OCI-AML3 cells were transfected with the pEAK-Flag/INPP4B plasmids, western blotting analysis of INPP4B and NPM1-mA. Proteins were quantified using image software and normalized against β-actin. d CCK-8 assay analysis of cell proliferation in NPM1-mA-silenced OCI-AML3 cells, followed by Flag-INPP4B introduction. Data were represented as mean ± s.d. of three individual experiments. * p
Figure Legend Snippet: NPM1-mA-mediated INPP4B upregulation promotes cell proliferation in OCI-AML3 cells. The NPM1-mA-silenced OCI-AML3 cells were subjected to ( a ) CCK8 assays and ( b ) colony forming assays. c The NPM1-mA-silenced OCI-AML3 cells were transfected with the pEAK-Flag/INPP4B plasmids, western blotting analysis of INPP4B and NPM1-mA. Proteins were quantified using image software and normalized against β-actin. d CCK-8 assay analysis of cell proliferation in NPM1-mA-silenced OCI-AML3 cells, followed by Flag-INPP4B introduction. Data were represented as mean ± s.d. of three individual experiments. * p

Techniques Used: Transfection, Western Blot, Software, CCK-8 Assay

High expression of INPP4B is associated with poor survival outcome in NPM1-mutated leukemia. Kaplan-Meier survival data of 153 AML patients were used to analysis ( a ) OS and ( b ) EFS curves according to INPP4B levels. c Heatmap of 38 primary AML samples with NPM1 mutation from TCGA dataset in which INPP4B expression was aligned with patient event (Survival or Death). Kaplan-Meier survival data of patients with NPM1-mutated AML were used to analysis ( d ) OS and ( e ) EFS curves according to INPP4B levels. f Schematic diagram describing the functional significance of INPP4B in the NPM1-mutated leukemia cells. INPP4B promotes leukemia cell survival in a SGK3-dependent and AKT-independent manner. The expression of INPP4B partially upregulated by NPM1-mA is due to ERK/Ets-1 signaling
Figure Legend Snippet: High expression of INPP4B is associated with poor survival outcome in NPM1-mutated leukemia. Kaplan-Meier survival data of 153 AML patients were used to analysis ( a ) OS and ( b ) EFS curves according to INPP4B levels. c Heatmap of 38 primary AML samples with NPM1 mutation from TCGA dataset in which INPP4B expression was aligned with patient event (Survival or Death). Kaplan-Meier survival data of patients with NPM1-mutated AML were used to analysis ( d ) OS and ( e ) EFS curves according to INPP4B levels. f Schematic diagram describing the functional significance of INPP4B in the NPM1-mutated leukemia cells. INPP4B promotes leukemia cell survival in a SGK3-dependent and AKT-independent manner. The expression of INPP4B partially upregulated by NPM1-mA is due to ERK/Ets-1 signaling

Techniques Used: Expressing, Mutagenesis, Functional Assay

High expression levels of INPP4B in leukemia cells with the NPM1 mutation. a RNA-seq mRNA expression data from the TCGA database were used to compare INPP4B expression between AML patients with ( n = 41) and without NPM1 mutation ( n = 130). * p
Figure Legend Snippet: High expression levels of INPP4B in leukemia cells with the NPM1 mutation. a RNA-seq mRNA expression data from the TCGA database were used to compare INPP4B expression between AML patients with ( n = 41) and without NPM1 mutation ( n = 130). * p

Techniques Used: Expressing, Mutagenesis, RNA Sequencing Assay

25) Product Images from "INPP4B promotes cell survival via SGK3 activation in NPM1-mutated leukemia"

Article Title: INPP4B promotes cell survival via SGK3 activation in NPM1-mutated leukemia

Journal: Journal of Experimental & Clinical Cancer Research : CR

doi: 10.1186/s13046-018-0675-9

INPP4B is upregulated by NPM1-mA in leukemia cells via ERK/Ets-1 signaling. ( a ) qRT-PCR analysis of INPP4B mRNA expression, ( b ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. ( c ) qRT-PCR analysis of INPP4B mRNA expression, ( d ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the THP-1 and K562 cells transduced with the plasmids expressing NPM1-wt or NPM1-mA. ( e ) qRT-PCR analysis of INPP4B mRNA expression, ( f ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3, p-Ets-1 and Ets-1 from the OCI-AML3 cells transfected with the control siRNA or siEts-1. ( g ) Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 and SGK3, ( h ) qRT-PCR analysis of INPP4B mRNA expression from the OCI-AML3 cells treated with different concentration of PD98059 (0, 10, 20 and 40 μM). i Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. Proteins were quantified using image software and normalized against β-actin. Data were represented as mean ± s.d. of three individual experiments. * p
Figure Legend Snippet: INPP4B is upregulated by NPM1-mA in leukemia cells via ERK/Ets-1 signaling. ( a ) qRT-PCR analysis of INPP4B mRNA expression, ( b ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. ( c ) qRT-PCR analysis of INPP4B mRNA expression, ( d ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the THP-1 and K562 cells transduced with the plasmids expressing NPM1-wt or NPM1-mA. ( e ) qRT-PCR analysis of INPP4B mRNA expression, ( f ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3, p-Ets-1 and Ets-1 from the OCI-AML3 cells transfected with the control siRNA or siEts-1. ( g ) Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 and SGK3, ( h ) qRT-PCR analysis of INPP4B mRNA expression from the OCI-AML3 cells treated with different concentration of PD98059 (0, 10, 20 and 40 μM). i Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. Proteins were quantified using image software and normalized against β-actin. Data were represented as mean ± s.d. of three individual experiments. * p

Techniques Used: Quantitative RT-PCR, Expressing, Western Blot, Transduction, Transfection, Concentration Assay, Software

NPM1-mA-mediated INPP4B upregulation promotes cell proliferation in OCI-AML3 cells. The NPM1-mA-silenced OCI-AML3 cells were subjected to ( a ) CCK8 assays and ( b ) colony forming assays. c The NPM1-mA-silenced OCI-AML3 cells were transfected with the pEAK-Flag/INPP4B plasmids, western blotting analysis of INPP4B and NPM1-mA. Proteins were quantified using image software and normalized against β-actin. d CCK-8 assay analysis of cell proliferation in NPM1-mA-silenced OCI-AML3 cells, followed by Flag-INPP4B introduction. Data were represented as mean ± s.d. of three individual experiments. * p
Figure Legend Snippet: NPM1-mA-mediated INPP4B upregulation promotes cell proliferation in OCI-AML3 cells. The NPM1-mA-silenced OCI-AML3 cells were subjected to ( a ) CCK8 assays and ( b ) colony forming assays. c The NPM1-mA-silenced OCI-AML3 cells were transfected with the pEAK-Flag/INPP4B plasmids, western blotting analysis of INPP4B and NPM1-mA. Proteins were quantified using image software and normalized against β-actin. d CCK-8 assay analysis of cell proliferation in NPM1-mA-silenced OCI-AML3 cells, followed by Flag-INPP4B introduction. Data were represented as mean ± s.d. of three individual experiments. * p

Techniques Used: Transfection, Western Blot, Software, CCK-8 Assay

High expression of INPP4B is associated with poor survival outcome in NPM1-mutated leukemia. Kaplan-Meier survival data of 153 AML patients were used to analysis ( a ) OS and ( b ) EFS curves according to INPP4B levels. c Heatmap of 38 primary AML samples with NPM1 mutation from TCGA dataset in which INPP4B expression was aligned with patient event (Survival or Death). Kaplan-Meier survival data of patients with NPM1-mutated AML were used to analysis ( d ) OS and ( e ) EFS curves according to INPP4B levels. f Schematic diagram describing the functional significance of INPP4B in the NPM1-mutated leukemia cells. INPP4B promotes leukemia cell survival in a SGK3-dependent and AKT-independent manner. The expression of INPP4B partially upregulated by NPM1-mA is due to ERK/Ets-1 signaling
Figure Legend Snippet: High expression of INPP4B is associated with poor survival outcome in NPM1-mutated leukemia. Kaplan-Meier survival data of 153 AML patients were used to analysis ( a ) OS and ( b ) EFS curves according to INPP4B levels. c Heatmap of 38 primary AML samples with NPM1 mutation from TCGA dataset in which INPP4B expression was aligned with patient event (Survival or Death). Kaplan-Meier survival data of patients with NPM1-mutated AML were used to analysis ( d ) OS and ( e ) EFS curves according to INPP4B levels. f Schematic diagram describing the functional significance of INPP4B in the NPM1-mutated leukemia cells. INPP4B promotes leukemia cell survival in a SGK3-dependent and AKT-independent manner. The expression of INPP4B partially upregulated by NPM1-mA is due to ERK/Ets-1 signaling

Techniques Used: Expressing, Mutagenesis, Functional Assay

High expression levels of INPP4B in leukemia cells with the NPM1 mutation. a RNA-seq mRNA expression data from the TCGA database were used to compare INPP4B expression between AML patients with ( n = 41) and without NPM1 mutation ( n = 130). * p
Figure Legend Snippet: High expression levels of INPP4B in leukemia cells with the NPM1 mutation. a RNA-seq mRNA expression data from the TCGA database were used to compare INPP4B expression between AML patients with ( n = 41) and without NPM1 mutation ( n = 130). * p

Techniques Used: Expressing, Mutagenesis, RNA Sequencing Assay

26) Product Images from "INPP4B promotes cell survival via SGK3 activation in NPM1-mutated leukemia"

Article Title: INPP4B promotes cell survival via SGK3 activation in NPM1-mutated leukemia

Journal: Journal of Experimental & Clinical Cancer Research : CR

doi: 10.1186/s13046-018-0675-9

INPP4B is upregulated by NPM1-mA in leukemia cells via ERK/Ets-1 signaling. ( a ) qRT-PCR analysis of INPP4B mRNA expression, ( b ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. ( c ) qRT-PCR analysis of INPP4B mRNA expression, ( d ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the THP-1 and K562 cells transduced with the plasmids expressing NPM1-wt or NPM1-mA. ( e ) qRT-PCR analysis of INPP4B mRNA expression, ( f ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3, p-Ets-1 and Ets-1 from the OCI-AML3 cells transfected with the control siRNA or siEts-1. ( g ) Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 and SGK3, ( h ) qRT-PCR analysis of INPP4B mRNA expression from the OCI-AML3 cells treated with different concentration of PD98059 (0, 10, 20 and 40 μM). i Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. Proteins were quantified using image software and normalized against β-actin. Data were represented as mean ± s.d. of three individual experiments. * p
Figure Legend Snippet: INPP4B is upregulated by NPM1-mA in leukemia cells via ERK/Ets-1 signaling. ( a ) qRT-PCR analysis of INPP4B mRNA expression, ( b ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. ( c ) qRT-PCR analysis of INPP4B mRNA expression, ( d ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the THP-1 and K562 cells transduced with the plasmids expressing NPM1-wt or NPM1-mA. ( e ) qRT-PCR analysis of INPP4B mRNA expression, ( f ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3, p-Ets-1 and Ets-1 from the OCI-AML3 cells transfected with the control siRNA or siEts-1. ( g ) Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 and SGK3, ( h ) qRT-PCR analysis of INPP4B mRNA expression from the OCI-AML3 cells treated with different concentration of PD98059 (0, 10, 20 and 40 μM). i Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. Proteins were quantified using image software and normalized against β-actin. Data were represented as mean ± s.d. of three individual experiments. * p

Techniques Used: Quantitative RT-PCR, Expressing, Western Blot, Transduction, Transfection, Concentration Assay, Software

NPM1-mA-mediated INPP4B upregulation promotes cell proliferation in OCI-AML3 cells. The NPM1-mA-silenced OCI-AML3 cells were subjected to ( a ) CCK8 assays and ( b ) colony forming assays. c The NPM1-mA-silenced OCI-AML3 cells were transfected with the pEAK-Flag/INPP4B plasmids, western blotting analysis of INPP4B and NPM1-mA. Proteins were quantified using image software and normalized against β-actin. d CCK-8 assay analysis of cell proliferation in NPM1-mA-silenced OCI-AML3 cells, followed by Flag-INPP4B introduction. Data were represented as mean ± s.d. of three individual experiments. * p
Figure Legend Snippet: NPM1-mA-mediated INPP4B upregulation promotes cell proliferation in OCI-AML3 cells. The NPM1-mA-silenced OCI-AML3 cells were subjected to ( a ) CCK8 assays and ( b ) colony forming assays. c The NPM1-mA-silenced OCI-AML3 cells were transfected with the pEAK-Flag/INPP4B plasmids, western blotting analysis of INPP4B and NPM1-mA. Proteins were quantified using image software and normalized against β-actin. d CCK-8 assay analysis of cell proliferation in NPM1-mA-silenced OCI-AML3 cells, followed by Flag-INPP4B introduction. Data were represented as mean ± s.d. of three individual experiments. * p

Techniques Used: Transfection, Western Blot, Software, CCK-8 Assay

High expression of INPP4B is associated with poor survival outcome in NPM1-mutated leukemia. Kaplan-Meier survival data of 153 AML patients were used to analysis ( a ) OS and ( b ) EFS curves according to INPP4B levels. c Heatmap of 38 primary AML samples with NPM1 mutation from TCGA dataset in which INPP4B expression was aligned with patient event (Survival or Death). Kaplan-Meier survival data of patients with NPM1-mutated AML were used to analysis ( d ) OS and ( e ) EFS curves according to INPP4B levels. f Schematic diagram describing the functional significance of INPP4B in the NPM1-mutated leukemia cells. INPP4B promotes leukemia cell survival in a SGK3-dependent and AKT-independent manner. The expression of INPP4B partially upregulated by NPM1-mA is due to ERK/Ets-1 signaling
Figure Legend Snippet: High expression of INPP4B is associated with poor survival outcome in NPM1-mutated leukemia. Kaplan-Meier survival data of 153 AML patients were used to analysis ( a ) OS and ( b ) EFS curves according to INPP4B levels. c Heatmap of 38 primary AML samples with NPM1 mutation from TCGA dataset in which INPP4B expression was aligned with patient event (Survival or Death). Kaplan-Meier survival data of patients with NPM1-mutated AML were used to analysis ( d ) OS and ( e ) EFS curves according to INPP4B levels. f Schematic diagram describing the functional significance of INPP4B in the NPM1-mutated leukemia cells. INPP4B promotes leukemia cell survival in a SGK3-dependent and AKT-independent manner. The expression of INPP4B partially upregulated by NPM1-mA is due to ERK/Ets-1 signaling

Techniques Used: Expressing, Mutagenesis, Functional Assay

High expression levels of INPP4B in leukemia cells with the NPM1 mutation. a RNA-seq mRNA expression data from the TCGA database were used to compare INPP4B expression between AML patients with ( n = 41) and without NPM1 mutation ( n = 130). * p
Figure Legend Snippet: High expression levels of INPP4B in leukemia cells with the NPM1 mutation. a RNA-seq mRNA expression data from the TCGA database were used to compare INPP4B expression between AML patients with ( n = 41) and without NPM1 mutation ( n = 130). * p

Techniques Used: Expressing, Mutagenesis, RNA Sequencing Assay

27) Product Images from "INPP4B promotes cell survival via SGK3 activation in NPM1-mutated leukemia"

Article Title: INPP4B promotes cell survival via SGK3 activation in NPM1-mutated leukemia

Journal: Journal of Experimental & Clinical Cancer Research : CR

doi: 10.1186/s13046-018-0675-9

INPP4B is upregulated by NPM1-mA in leukemia cells via ERK/Ets-1 signaling. ( a ) qRT-PCR analysis of INPP4B mRNA expression, ( b ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. ( c ) qRT-PCR analysis of INPP4B mRNA expression, ( d ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the THP-1 and K562 cells transduced with the plasmids expressing NPM1-wt or NPM1-mA. ( e ) qRT-PCR analysis of INPP4B mRNA expression, ( f ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3, p-Ets-1 and Ets-1 from the OCI-AML3 cells transfected with the control siRNA or siEts-1. ( g ) Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 and SGK3, ( h ) qRT-PCR analysis of INPP4B mRNA expression from the OCI-AML3 cells treated with different concentration of PD98059 (0, 10, 20 and 40 μM). i Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. Proteins were quantified using image software and normalized against β-actin. Data were represented as mean ± s.d. of three individual experiments. * p
Figure Legend Snippet: INPP4B is upregulated by NPM1-mA in leukemia cells via ERK/Ets-1 signaling. ( a ) qRT-PCR analysis of INPP4B mRNA expression, ( b ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. ( c ) qRT-PCR analysis of INPP4B mRNA expression, ( d ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the THP-1 and K562 cells transduced with the plasmids expressing NPM1-wt or NPM1-mA. ( e ) qRT-PCR analysis of INPP4B mRNA expression, ( f ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3, p-Ets-1 and Ets-1 from the OCI-AML3 cells transfected with the control siRNA or siEts-1. ( g ) Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 and SGK3, ( h ) qRT-PCR analysis of INPP4B mRNA expression from the OCI-AML3 cells treated with different concentration of PD98059 (0, 10, 20 and 40 μM). i Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. Proteins were quantified using image software and normalized against β-actin. Data were represented as mean ± s.d. of three individual experiments. * p

Techniques Used: Quantitative RT-PCR, Expressing, Western Blot, Transduction, Transfection, Concentration Assay, Software

NPM1-mA-mediated INPP4B upregulation promotes cell proliferation in OCI-AML3 cells. The NPM1-mA-silenced OCI-AML3 cells were subjected to ( a ) CCK8 assays and ( b ) colony forming assays. c The NPM1-mA-silenced OCI-AML3 cells were transfected with the pEAK-Flag/INPP4B plasmids, western blotting analysis of INPP4B and NPM1-mA. Proteins were quantified using image software and normalized against β-actin. d CCK-8 assay analysis of cell proliferation in NPM1-mA-silenced OCI-AML3 cells, followed by Flag-INPP4B introduction. Data were represented as mean ± s.d. of three individual experiments. * p
Figure Legend Snippet: NPM1-mA-mediated INPP4B upregulation promotes cell proliferation in OCI-AML3 cells. The NPM1-mA-silenced OCI-AML3 cells were subjected to ( a ) CCK8 assays and ( b ) colony forming assays. c The NPM1-mA-silenced OCI-AML3 cells were transfected with the pEAK-Flag/INPP4B plasmids, western blotting analysis of INPP4B and NPM1-mA. Proteins were quantified using image software and normalized against β-actin. d CCK-8 assay analysis of cell proliferation in NPM1-mA-silenced OCI-AML3 cells, followed by Flag-INPP4B introduction. Data were represented as mean ± s.d. of three individual experiments. * p

Techniques Used: Transfection, Western Blot, Software, CCK-8 Assay

High expression of INPP4B is associated with poor survival outcome in NPM1-mutated leukemia. Kaplan-Meier survival data of 153 AML patients were used to analysis ( a ) OS and ( b ) EFS curves according to INPP4B levels. c Heatmap of 38 primary AML samples with NPM1 mutation from TCGA dataset in which INPP4B expression was aligned with patient event (Survival or Death). Kaplan-Meier survival data of patients with NPM1-mutated AML were used to analysis ( d ) OS and ( e ) EFS curves according to INPP4B levels. f Schematic diagram describing the functional significance of INPP4B in the NPM1-mutated leukemia cells. INPP4B promotes leukemia cell survival in a SGK3-dependent and AKT-independent manner. The expression of INPP4B partially upregulated by NPM1-mA is due to ERK/Ets-1 signaling
Figure Legend Snippet: High expression of INPP4B is associated with poor survival outcome in NPM1-mutated leukemia. Kaplan-Meier survival data of 153 AML patients were used to analysis ( a ) OS and ( b ) EFS curves according to INPP4B levels. c Heatmap of 38 primary AML samples with NPM1 mutation from TCGA dataset in which INPP4B expression was aligned with patient event (Survival or Death). Kaplan-Meier survival data of patients with NPM1-mutated AML were used to analysis ( d ) OS and ( e ) EFS curves according to INPP4B levels. f Schematic diagram describing the functional significance of INPP4B in the NPM1-mutated leukemia cells. INPP4B promotes leukemia cell survival in a SGK3-dependent and AKT-independent manner. The expression of INPP4B partially upregulated by NPM1-mA is due to ERK/Ets-1 signaling

Techniques Used: Expressing, Mutagenesis, Functional Assay

High expression levels of INPP4B in leukemia cells with the NPM1 mutation. a RNA-seq mRNA expression data from the TCGA database were used to compare INPP4B expression between AML patients with ( n = 41) and without NPM1 mutation ( n = 130). * p
Figure Legend Snippet: High expression levels of INPP4B in leukemia cells with the NPM1 mutation. a RNA-seq mRNA expression data from the TCGA database were used to compare INPP4B expression between AML patients with ( n = 41) and without NPM1 mutation ( n = 130). * p

Techniques Used: Expressing, Mutagenesis, RNA Sequencing Assay

28) Product Images from "INPP4B promotes cell survival via SGK3 activation in NPM1-mutated leukemia"

Article Title: INPP4B promotes cell survival via SGK3 activation in NPM1-mutated leukemia

Journal: Journal of Experimental & Clinical Cancer Research : CR

doi: 10.1186/s13046-018-0675-9

INPP4B is upregulated by NPM1-mA in leukemia cells via ERK/Ets-1 signaling. ( a ) qRT-PCR analysis of INPP4B mRNA expression, ( b ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. ( c ) qRT-PCR analysis of INPP4B mRNA expression, ( d ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the THP-1 and K562 cells transduced with the plasmids expressing NPM1-wt or NPM1-mA. ( e ) qRT-PCR analysis of INPP4B mRNA expression, ( f ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3, p-Ets-1 and Ets-1 from the OCI-AML3 cells transfected with the control siRNA or siEts-1. ( g ) Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 and SGK3, ( h ) qRT-PCR analysis of INPP4B mRNA expression from the OCI-AML3 cells treated with different concentration of PD98059 (0, 10, 20 and 40 μM). i Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. Proteins were quantified using image software and normalized against β-actin. Data were represented as mean ± s.d. of three individual experiments. * p
Figure Legend Snippet: INPP4B is upregulated by NPM1-mA in leukemia cells via ERK/Ets-1 signaling. ( a ) qRT-PCR analysis of INPP4B mRNA expression, ( b ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. ( c ) qRT-PCR analysis of INPP4B mRNA expression, ( d ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the THP-1 and K562 cells transduced with the plasmids expressing NPM1-wt or NPM1-mA. ( e ) qRT-PCR analysis of INPP4B mRNA expression, ( f ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3, p-Ets-1 and Ets-1 from the OCI-AML3 cells transfected with the control siRNA or siEts-1. ( g ) Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 and SGK3, ( h ) qRT-PCR analysis of INPP4B mRNA expression from the OCI-AML3 cells treated with different concentration of PD98059 (0, 10, 20 and 40 μM). i Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. Proteins were quantified using image software and normalized against β-actin. Data were represented as mean ± s.d. of three individual experiments. * p

Techniques Used: Quantitative RT-PCR, Expressing, Western Blot, Transduction, Transfection, Concentration Assay, Software

NPM1-mA-mediated INPP4B upregulation promotes cell proliferation in OCI-AML3 cells. The NPM1-mA-silenced OCI-AML3 cells were subjected to ( a ) CCK8 assays and ( b ) colony forming assays. c The NPM1-mA-silenced OCI-AML3 cells were transfected with the pEAK-Flag/INPP4B plasmids, western blotting analysis of INPP4B and NPM1-mA. Proteins were quantified using image software and normalized against β-actin. d CCK-8 assay analysis of cell proliferation in NPM1-mA-silenced OCI-AML3 cells, followed by Flag-INPP4B introduction. Data were represented as mean ± s.d. of three individual experiments. * p
Figure Legend Snippet: NPM1-mA-mediated INPP4B upregulation promotes cell proliferation in OCI-AML3 cells. The NPM1-mA-silenced OCI-AML3 cells were subjected to ( a ) CCK8 assays and ( b ) colony forming assays. c The NPM1-mA-silenced OCI-AML3 cells were transfected with the pEAK-Flag/INPP4B plasmids, western blotting analysis of INPP4B and NPM1-mA. Proteins were quantified using image software and normalized against β-actin. d CCK-8 assay analysis of cell proliferation in NPM1-mA-silenced OCI-AML3 cells, followed by Flag-INPP4B introduction. Data were represented as mean ± s.d. of three individual experiments. * p

Techniques Used: Transfection, Western Blot, Software, CCK-8 Assay

High expression of INPP4B is associated with poor survival outcome in NPM1-mutated leukemia. Kaplan-Meier survival data of 153 AML patients were used to analysis ( a ) OS and ( b ) EFS curves according to INPP4B levels. c Heatmap of 38 primary AML samples with NPM1 mutation from TCGA dataset in which INPP4B expression was aligned with patient event (Survival or Death). Kaplan-Meier survival data of patients with NPM1-mutated AML were used to analysis ( d ) OS and ( e ) EFS curves according to INPP4B levels. f Schematic diagram describing the functional significance of INPP4B in the NPM1-mutated leukemia cells. INPP4B promotes leukemia cell survival in a SGK3-dependent and AKT-independent manner. The expression of INPP4B partially upregulated by NPM1-mA is due to ERK/Ets-1 signaling
Figure Legend Snippet: High expression of INPP4B is associated with poor survival outcome in NPM1-mutated leukemia. Kaplan-Meier survival data of 153 AML patients were used to analysis ( a ) OS and ( b ) EFS curves according to INPP4B levels. c Heatmap of 38 primary AML samples with NPM1 mutation from TCGA dataset in which INPP4B expression was aligned with patient event (Survival or Death). Kaplan-Meier survival data of patients with NPM1-mutated AML were used to analysis ( d ) OS and ( e ) EFS curves according to INPP4B levels. f Schematic diagram describing the functional significance of INPP4B in the NPM1-mutated leukemia cells. INPP4B promotes leukemia cell survival in a SGK3-dependent and AKT-independent manner. The expression of INPP4B partially upregulated by NPM1-mA is due to ERK/Ets-1 signaling

Techniques Used: Expressing, Mutagenesis, Functional Assay

High expression levels of INPP4B in leukemia cells with the NPM1 mutation. a RNA-seq mRNA expression data from the TCGA database were used to compare INPP4B expression between AML patients with ( n = 41) and without NPM1 mutation ( n = 130). * p
Figure Legend Snippet: High expression levels of INPP4B in leukemia cells with the NPM1 mutation. a RNA-seq mRNA expression data from the TCGA database were used to compare INPP4B expression between AML patients with ( n = 41) and without NPM1 mutation ( n = 130). * p

Techniques Used: Expressing, Mutagenesis, RNA Sequencing Assay

29) Product Images from "INPP4B promotes cell survival via SGK3 activation in NPM1-mutated leukemia"

Article Title: INPP4B promotes cell survival via SGK3 activation in NPM1-mutated leukemia

Journal: Journal of Experimental & Clinical Cancer Research : CR

doi: 10.1186/s13046-018-0675-9

INPP4B is upregulated by NPM1-mA in leukemia cells via ERK/Ets-1 signaling. ( a ) qRT-PCR analysis of INPP4B mRNA expression, ( b ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. ( c ) qRT-PCR analysis of INPP4B mRNA expression, ( d ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the THP-1 and K562 cells transduced with the plasmids expressing NPM1-wt or NPM1-mA. ( e ) qRT-PCR analysis of INPP4B mRNA expression, ( f ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3, p-Ets-1 and Ets-1 from the OCI-AML3 cells transfected with the control siRNA or siEts-1. ( g ) Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 and SGK3, ( h ) qRT-PCR analysis of INPP4B mRNA expression from the OCI-AML3 cells treated with different concentration of PD98059 (0, 10, 20 and 40 μM). i Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. Proteins were quantified using image software and normalized against β-actin. Data were represented as mean ± s.d. of three individual experiments. * p
Figure Legend Snippet: INPP4B is upregulated by NPM1-mA in leukemia cells via ERK/Ets-1 signaling. ( a ) qRT-PCR analysis of INPP4B mRNA expression, ( b ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. ( c ) qRT-PCR analysis of INPP4B mRNA expression, ( d ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the THP-1 and K562 cells transduced with the plasmids expressing NPM1-wt or NPM1-mA. ( e ) qRT-PCR analysis of INPP4B mRNA expression, ( f ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3, p-Ets-1 and Ets-1 from the OCI-AML3 cells transfected with the control siRNA or siEts-1. ( g ) Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 and SGK3, ( h ) qRT-PCR analysis of INPP4B mRNA expression from the OCI-AML3 cells treated with different concentration of PD98059 (0, 10, 20 and 40 μM). i Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. Proteins were quantified using image software and normalized against β-actin. Data were represented as mean ± s.d. of three individual experiments. * p

Techniques Used: Quantitative RT-PCR, Expressing, Western Blot, Transduction, Transfection, Concentration Assay, Software

NPM1-mA-mediated INPP4B upregulation promotes cell proliferation in OCI-AML3 cells. The NPM1-mA-silenced OCI-AML3 cells were subjected to ( a ) CCK8 assays and ( b ) colony forming assays. c The NPM1-mA-silenced OCI-AML3 cells were transfected with the pEAK-Flag/INPP4B plasmids, western blotting analysis of INPP4B and NPM1-mA. Proteins were quantified using image software and normalized against β-actin. d CCK-8 assay analysis of cell proliferation in NPM1-mA-silenced OCI-AML3 cells, followed by Flag-INPP4B introduction. Data were represented as mean ± s.d. of three individual experiments. * p
Figure Legend Snippet: NPM1-mA-mediated INPP4B upregulation promotes cell proliferation in OCI-AML3 cells. The NPM1-mA-silenced OCI-AML3 cells were subjected to ( a ) CCK8 assays and ( b ) colony forming assays. c The NPM1-mA-silenced OCI-AML3 cells were transfected with the pEAK-Flag/INPP4B plasmids, western blotting analysis of INPP4B and NPM1-mA. Proteins were quantified using image software and normalized against β-actin. d CCK-8 assay analysis of cell proliferation in NPM1-mA-silenced OCI-AML3 cells, followed by Flag-INPP4B introduction. Data were represented as mean ± s.d. of three individual experiments. * p

Techniques Used: Transfection, Western Blot, Software, CCK-8 Assay

High expression of INPP4B is associated with poor survival outcome in NPM1-mutated leukemia. Kaplan-Meier survival data of 153 AML patients were used to analysis ( a ) OS and ( b ) EFS curves according to INPP4B levels. c Heatmap of 38 primary AML samples with NPM1 mutation from TCGA dataset in which INPP4B expression was aligned with patient event (Survival or Death). Kaplan-Meier survival data of patients with NPM1-mutated AML were used to analysis ( d ) OS and ( e ) EFS curves according to INPP4B levels. f Schematic diagram describing the functional significance of INPP4B in the NPM1-mutated leukemia cells. INPP4B promotes leukemia cell survival in a SGK3-dependent and AKT-independent manner. The expression of INPP4B partially upregulated by NPM1-mA is due to ERK/Ets-1 signaling
Figure Legend Snippet: High expression of INPP4B is associated with poor survival outcome in NPM1-mutated leukemia. Kaplan-Meier survival data of 153 AML patients were used to analysis ( a ) OS and ( b ) EFS curves according to INPP4B levels. c Heatmap of 38 primary AML samples with NPM1 mutation from TCGA dataset in which INPP4B expression was aligned with patient event (Survival or Death). Kaplan-Meier survival data of patients with NPM1-mutated AML were used to analysis ( d ) OS and ( e ) EFS curves according to INPP4B levels. f Schematic diagram describing the functional significance of INPP4B in the NPM1-mutated leukemia cells. INPP4B promotes leukemia cell survival in a SGK3-dependent and AKT-independent manner. The expression of INPP4B partially upregulated by NPM1-mA is due to ERK/Ets-1 signaling

Techniques Used: Expressing, Mutagenesis, Functional Assay

High expression levels of INPP4B in leukemia cells with the NPM1 mutation. a RNA-seq mRNA expression data from the TCGA database were used to compare INPP4B expression between AML patients with ( n = 41) and without NPM1 mutation ( n = 130). * p
Figure Legend Snippet: High expression levels of INPP4B in leukemia cells with the NPM1 mutation. a RNA-seq mRNA expression data from the TCGA database were used to compare INPP4B expression between AML patients with ( n = 41) and without NPM1 mutation ( n = 130). * p

Techniques Used: Expressing, Mutagenesis, RNA Sequencing Assay

30) Product Images from "INPP4B promotes cell survival via SGK3 activation in NPM1-mutated leukemia"

Article Title: INPP4B promotes cell survival via SGK3 activation in NPM1-mutated leukemia

Journal: Journal of Experimental & Clinical Cancer Research : CR

doi: 10.1186/s13046-018-0675-9

INPP4B is upregulated by NPM1-mA in leukemia cells via ERK/Ets-1 signaling. ( a ) qRT-PCR analysis of INPP4B mRNA expression, ( b ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. ( c ) qRT-PCR analysis of INPP4B mRNA expression, ( d ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the THP-1 and K562 cells transduced with the plasmids expressing NPM1-wt or NPM1-mA. ( e ) qRT-PCR analysis of INPP4B mRNA expression, ( f ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3, p-Ets-1 and Ets-1 from the OCI-AML3 cells transfected with the control siRNA or siEts-1. ( g ) Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 and SGK3, ( h ) qRT-PCR analysis of INPP4B mRNA expression from the OCI-AML3 cells treated with different concentration of PD98059 (0, 10, 20 and 40 μM). i Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. Proteins were quantified using image software and normalized against β-actin. Data were represented as mean ± s.d. of three individual experiments. * p
Figure Legend Snippet: INPP4B is upregulated by NPM1-mA in leukemia cells via ERK/Ets-1 signaling. ( a ) qRT-PCR analysis of INPP4B mRNA expression, ( b ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. ( c ) qRT-PCR analysis of INPP4B mRNA expression, ( d ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the THP-1 and K562 cells transduced with the plasmids expressing NPM1-wt or NPM1-mA. ( e ) qRT-PCR analysis of INPP4B mRNA expression, ( f ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3, p-Ets-1 and Ets-1 from the OCI-AML3 cells transfected with the control siRNA or siEts-1. ( g ) Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 and SGK3, ( h ) qRT-PCR analysis of INPP4B mRNA expression from the OCI-AML3 cells treated with different concentration of PD98059 (0, 10, 20 and 40 μM). i Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. Proteins were quantified using image software and normalized against β-actin. Data were represented as mean ± s.d. of three individual experiments. * p

Techniques Used: Quantitative RT-PCR, Expressing, Western Blot, Transduction, Transfection, Concentration Assay, Software

NPM1-mA-mediated INPP4B upregulation promotes cell proliferation in OCI-AML3 cells. The NPM1-mA-silenced OCI-AML3 cells were subjected to ( a ) CCK8 assays and ( b ) colony forming assays. c The NPM1-mA-silenced OCI-AML3 cells were transfected with the pEAK-Flag/INPP4B plasmids, western blotting analysis of INPP4B and NPM1-mA. Proteins were quantified using image software and normalized against β-actin. d CCK-8 assay analysis of cell proliferation in NPM1-mA-silenced OCI-AML3 cells, followed by Flag-INPP4B introduction. Data were represented as mean ± s.d. of three individual experiments. * p
Figure Legend Snippet: NPM1-mA-mediated INPP4B upregulation promotes cell proliferation in OCI-AML3 cells. The NPM1-mA-silenced OCI-AML3 cells were subjected to ( a ) CCK8 assays and ( b ) colony forming assays. c The NPM1-mA-silenced OCI-AML3 cells were transfected with the pEAK-Flag/INPP4B plasmids, western blotting analysis of INPP4B and NPM1-mA. Proteins were quantified using image software and normalized against β-actin. d CCK-8 assay analysis of cell proliferation in NPM1-mA-silenced OCI-AML3 cells, followed by Flag-INPP4B introduction. Data were represented as mean ± s.d. of three individual experiments. * p

Techniques Used: Transfection, Western Blot, Software, CCK-8 Assay

High expression of INPP4B is associated with poor survival outcome in NPM1-mutated leukemia. Kaplan-Meier survival data of 153 AML patients were used to analysis ( a ) OS and ( b ) EFS curves according to INPP4B levels. c Heatmap of 38 primary AML samples with NPM1 mutation from TCGA dataset in which INPP4B expression was aligned with patient event (Survival or Death). Kaplan-Meier survival data of patients with NPM1-mutated AML were used to analysis ( d ) OS and ( e ) EFS curves according to INPP4B levels. f Schematic diagram describing the functional significance of INPP4B in the NPM1-mutated leukemia cells. INPP4B promotes leukemia cell survival in a SGK3-dependent and AKT-independent manner. The expression of INPP4B partially upregulated by NPM1-mA is due to ERK/Ets-1 signaling
Figure Legend Snippet: High expression of INPP4B is associated with poor survival outcome in NPM1-mutated leukemia. Kaplan-Meier survival data of 153 AML patients were used to analysis ( a ) OS and ( b ) EFS curves according to INPP4B levels. c Heatmap of 38 primary AML samples with NPM1 mutation from TCGA dataset in which INPP4B expression was aligned with patient event (Survival or Death). Kaplan-Meier survival data of patients with NPM1-mutated AML were used to analysis ( d ) OS and ( e ) EFS curves according to INPP4B levels. f Schematic diagram describing the functional significance of INPP4B in the NPM1-mutated leukemia cells. INPP4B promotes leukemia cell survival in a SGK3-dependent and AKT-independent manner. The expression of INPP4B partially upregulated by NPM1-mA is due to ERK/Ets-1 signaling

Techniques Used: Expressing, Mutagenesis, Functional Assay

High expression levels of INPP4B in leukemia cells with the NPM1 mutation. a RNA-seq mRNA expression data from the TCGA database were used to compare INPP4B expression between AML patients with ( n = 41) and without NPM1 mutation ( n = 130). * p
Figure Legend Snippet: High expression levels of INPP4B in leukemia cells with the NPM1 mutation. a RNA-seq mRNA expression data from the TCGA database were used to compare INPP4B expression between AML patients with ( n = 41) and without NPM1 mutation ( n = 130). * p

Techniques Used: Expressing, Mutagenesis, RNA Sequencing Assay

31) Product Images from "INPP4B promotes cell survival via SGK3 activation in NPM1-mutated leukemia"

Article Title: INPP4B promotes cell survival via SGK3 activation in NPM1-mutated leukemia

Journal: Journal of Experimental & Clinical Cancer Research : CR

doi: 10.1186/s13046-018-0675-9

INPP4B is upregulated by NPM1-mA in leukemia cells via ERK/Ets-1 signaling. ( a ) qRT-PCR analysis of INPP4B mRNA expression, ( b ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. ( c ) qRT-PCR analysis of INPP4B mRNA expression, ( d ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the THP-1 and K562 cells transduced with the plasmids expressing NPM1-wt or NPM1-mA. ( e ) qRT-PCR analysis of INPP4B mRNA expression, ( f ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3, p-Ets-1 and Ets-1 from the OCI-AML3 cells transfected with the control siRNA or siEts-1. ( g ) Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 and SGK3, ( h ) qRT-PCR analysis of INPP4B mRNA expression from the OCI-AML3 cells treated with different concentration of PD98059 (0, 10, 20 and 40 μM). i Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. Proteins were quantified using image software and normalized against β-actin. Data were represented as mean ± s.d. of three individual experiments. * p
Figure Legend Snippet: INPP4B is upregulated by NPM1-mA in leukemia cells via ERK/Ets-1 signaling. ( a ) qRT-PCR analysis of INPP4B mRNA expression, ( b ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. ( c ) qRT-PCR analysis of INPP4B mRNA expression, ( d ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the THP-1 and K562 cells transduced with the plasmids expressing NPM1-wt or NPM1-mA. ( e ) qRT-PCR analysis of INPP4B mRNA expression, ( f ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3, p-Ets-1 and Ets-1 from the OCI-AML3 cells transfected with the control siRNA or siEts-1. ( g ) Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 and SGK3, ( h ) qRT-PCR analysis of INPP4B mRNA expression from the OCI-AML3 cells treated with different concentration of PD98059 (0, 10, 20 and 40 μM). i Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. Proteins were quantified using image software and normalized against β-actin. Data were represented as mean ± s.d. of three individual experiments. * p

Techniques Used: Quantitative RT-PCR, Expressing, Western Blot, Transduction, Transfection, Concentration Assay, Software

NPM1-mA-mediated INPP4B upregulation promotes cell proliferation in OCI-AML3 cells. The NPM1-mA-silenced OCI-AML3 cells were subjected to ( a ) CCK8 assays and ( b ) colony forming assays. c The NPM1-mA-silenced OCI-AML3 cells were transfected with the pEAK-Flag/INPP4B plasmids, western blotting analysis of INPP4B and NPM1-mA. Proteins were quantified using image software and normalized against β-actin. d CCK-8 assay analysis of cell proliferation in NPM1-mA-silenced OCI-AML3 cells, followed by Flag-INPP4B introduction. Data were represented as mean ± s.d. of three individual experiments. * p
Figure Legend Snippet: NPM1-mA-mediated INPP4B upregulation promotes cell proliferation in OCI-AML3 cells. The NPM1-mA-silenced OCI-AML3 cells were subjected to ( a ) CCK8 assays and ( b ) colony forming assays. c The NPM1-mA-silenced OCI-AML3 cells were transfected with the pEAK-Flag/INPP4B plasmids, western blotting analysis of INPP4B and NPM1-mA. Proteins were quantified using image software and normalized against β-actin. d CCK-8 assay analysis of cell proliferation in NPM1-mA-silenced OCI-AML3 cells, followed by Flag-INPP4B introduction. Data were represented as mean ± s.d. of three individual experiments. * p

Techniques Used: Transfection, Western Blot, Software, CCK-8 Assay

High expression of INPP4B is associated with poor survival outcome in NPM1-mutated leukemia. Kaplan-Meier survival data of 153 AML patients were used to analysis ( a ) OS and ( b ) EFS curves according to INPP4B levels. c Heatmap of 38 primary AML samples with NPM1 mutation from TCGA dataset in which INPP4B expression was aligned with patient event (Survival or Death). Kaplan-Meier survival data of patients with NPM1-mutated AML were used to analysis ( d ) OS and ( e ) EFS curves according to INPP4B levels. f Schematic diagram describing the functional significance of INPP4B in the NPM1-mutated leukemia cells. INPP4B promotes leukemia cell survival in a SGK3-dependent and AKT-independent manner. The expression of INPP4B partially upregulated by NPM1-mA is due to ERK/Ets-1 signaling
Figure Legend Snippet: High expression of INPP4B is associated with poor survival outcome in NPM1-mutated leukemia. Kaplan-Meier survival data of 153 AML patients were used to analysis ( a ) OS and ( b ) EFS curves according to INPP4B levels. c Heatmap of 38 primary AML samples with NPM1 mutation from TCGA dataset in which INPP4B expression was aligned with patient event (Survival or Death). Kaplan-Meier survival data of patients with NPM1-mutated AML were used to analysis ( d ) OS and ( e ) EFS curves according to INPP4B levels. f Schematic diagram describing the functional significance of INPP4B in the NPM1-mutated leukemia cells. INPP4B promotes leukemia cell survival in a SGK3-dependent and AKT-independent manner. The expression of INPP4B partially upregulated by NPM1-mA is due to ERK/Ets-1 signaling

Techniques Used: Expressing, Mutagenesis, Functional Assay

High expression levels of INPP4B in leukemia cells with the NPM1 mutation. a RNA-seq mRNA expression data from the TCGA database were used to compare INPP4B expression between AML patients with ( n = 41) and without NPM1 mutation ( n = 130). * p
Figure Legend Snippet: High expression levels of INPP4B in leukemia cells with the NPM1 mutation. a RNA-seq mRNA expression data from the TCGA database were used to compare INPP4B expression between AML patients with ( n = 41) and without NPM1 mutation ( n = 130). * p

Techniques Used: Expressing, Mutagenesis, RNA Sequencing Assay

32) Product Images from "INPP4B promotes cell survival via SGK3 activation in NPM1-mutated leukemia"

Article Title: INPP4B promotes cell survival via SGK3 activation in NPM1-mutated leukemia

Journal: Journal of Experimental & Clinical Cancer Research : CR

doi: 10.1186/s13046-018-0675-9

INPP4B is upregulated by NPM1-mA in leukemia cells via ERK/Ets-1 signaling. ( a ) qRT-PCR analysis of INPP4B mRNA expression, ( b ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. ( c ) qRT-PCR analysis of INPP4B mRNA expression, ( d ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the THP-1 and K562 cells transduced with the plasmids expressing NPM1-wt or NPM1-mA. ( e ) qRT-PCR analysis of INPP4B mRNA expression, ( f ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3, p-Ets-1 and Ets-1 from the OCI-AML3 cells transfected with the control siRNA or siEts-1. ( g ) Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 and SGK3, ( h ) qRT-PCR analysis of INPP4B mRNA expression from the OCI-AML3 cells treated with different concentration of PD98059 (0, 10, 20 and 40 μM). i Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. Proteins were quantified using image software and normalized against β-actin. Data were represented as mean ± s.d. of three individual experiments. * p
Figure Legend Snippet: INPP4B is upregulated by NPM1-mA in leukemia cells via ERK/Ets-1 signaling. ( a ) qRT-PCR analysis of INPP4B mRNA expression, ( b ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. ( c ) qRT-PCR analysis of INPP4B mRNA expression, ( d ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the THP-1 and K562 cells transduced with the plasmids expressing NPM1-wt or NPM1-mA. ( e ) qRT-PCR analysis of INPP4B mRNA expression, ( f ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3, p-Ets-1 and Ets-1 from the OCI-AML3 cells transfected with the control siRNA or siEts-1. ( g ) Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 and SGK3, ( h ) qRT-PCR analysis of INPP4B mRNA expression from the OCI-AML3 cells treated with different concentration of PD98059 (0, 10, 20 and 40 μM). i Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. Proteins were quantified using image software and normalized against β-actin. Data were represented as mean ± s.d. of three individual experiments. * p

Techniques Used: Quantitative RT-PCR, Expressing, Western Blot, Transduction, Transfection, Concentration Assay, Software

NPM1-mA-mediated INPP4B upregulation promotes cell proliferation in OCI-AML3 cells. The NPM1-mA-silenced OCI-AML3 cells were subjected to ( a ) CCK8 assays and ( b ) colony forming assays. c The NPM1-mA-silenced OCI-AML3 cells were transfected with the pEAK-Flag/INPP4B plasmids, western blotting analysis of INPP4B and NPM1-mA. Proteins were quantified using image software and normalized against β-actin. d CCK-8 assay analysis of cell proliferation in NPM1-mA-silenced OCI-AML3 cells, followed by Flag-INPP4B introduction. Data were represented as mean ± s.d. of three individual experiments. * p
Figure Legend Snippet: NPM1-mA-mediated INPP4B upregulation promotes cell proliferation in OCI-AML3 cells. The NPM1-mA-silenced OCI-AML3 cells were subjected to ( a ) CCK8 assays and ( b ) colony forming assays. c The NPM1-mA-silenced OCI-AML3 cells were transfected with the pEAK-Flag/INPP4B plasmids, western blotting analysis of INPP4B and NPM1-mA. Proteins were quantified using image software and normalized against β-actin. d CCK-8 assay analysis of cell proliferation in NPM1-mA-silenced OCI-AML3 cells, followed by Flag-INPP4B introduction. Data were represented as mean ± s.d. of three individual experiments. * p

Techniques Used: Transfection, Western Blot, Software, CCK-8 Assay

High expression of INPP4B is associated with poor survival outcome in NPM1-mutated leukemia. Kaplan-Meier survival data of 153 AML patients were used to analysis ( a ) OS and ( b ) EFS curves according to INPP4B levels. c Heatmap of 38 primary AML samples with NPM1 mutation from TCGA dataset in which INPP4B expression was aligned with patient event (Survival or Death). Kaplan-Meier survival data of patients with NPM1-mutated AML were used to analysis ( d ) OS and ( e ) EFS curves according to INPP4B levels. f Schematic diagram describing the functional significance of INPP4B in the NPM1-mutated leukemia cells. INPP4B promotes leukemia cell survival in a SGK3-dependent and AKT-independent manner. The expression of INPP4B partially upregulated by NPM1-mA is due to ERK/Ets-1 signaling
Figure Legend Snippet: High expression of INPP4B is associated with poor survival outcome in NPM1-mutated leukemia. Kaplan-Meier survival data of 153 AML patients were used to analysis ( a ) OS and ( b ) EFS curves according to INPP4B levels. c Heatmap of 38 primary AML samples with NPM1 mutation from TCGA dataset in which INPP4B expression was aligned with patient event (Survival or Death). Kaplan-Meier survival data of patients with NPM1-mutated AML were used to analysis ( d ) OS and ( e ) EFS curves according to INPP4B levels. f Schematic diagram describing the functional significance of INPP4B in the NPM1-mutated leukemia cells. INPP4B promotes leukemia cell survival in a SGK3-dependent and AKT-independent manner. The expression of INPP4B partially upregulated by NPM1-mA is due to ERK/Ets-1 signaling

Techniques Used: Expressing, Mutagenesis, Functional Assay

High expression levels of INPP4B in leukemia cells with the NPM1 mutation. a RNA-seq mRNA expression data from the TCGA database were used to compare INPP4B expression between AML patients with ( n = 41) and without NPM1 mutation ( n = 130). * p
Figure Legend Snippet: High expression levels of INPP4B in leukemia cells with the NPM1 mutation. a RNA-seq mRNA expression data from the TCGA database were used to compare INPP4B expression between AML patients with ( n = 41) and without NPM1 mutation ( n = 130). * p

Techniques Used: Expressing, Mutagenesis, RNA Sequencing Assay

33) Product Images from "INPP4B promotes cell survival via SGK3 activation in NPM1-mutated leukemia"

Article Title: INPP4B promotes cell survival via SGK3 activation in NPM1-mutated leukemia

Journal: Journal of Experimental & Clinical Cancer Research : CR

doi: 10.1186/s13046-018-0675-9

INPP4B is upregulated by NPM1-mA in leukemia cells via ERK/Ets-1 signaling. ( a ) qRT-PCR analysis of INPP4B mRNA expression, ( b ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. ( c ) qRT-PCR analysis of INPP4B mRNA expression, ( d ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the THP-1 and K562 cells transduced with the plasmids expressing NPM1-wt or NPM1-mA. ( e ) qRT-PCR analysis of INPP4B mRNA expression, ( f ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3, p-Ets-1 and Ets-1 from the OCI-AML3 cells transfected with the control siRNA or siEts-1. ( g ) Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 and SGK3, ( h ) qRT-PCR analysis of INPP4B mRNA expression from the OCI-AML3 cells treated with different concentration of PD98059 (0, 10, 20 and 40 μM). i Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. Proteins were quantified using image software and normalized against β-actin. Data were represented as mean ± s.d. of three individual experiments. * p
Figure Legend Snippet: INPP4B is upregulated by NPM1-mA in leukemia cells via ERK/Ets-1 signaling. ( a ) qRT-PCR analysis of INPP4B mRNA expression, ( b ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. ( c ) qRT-PCR analysis of INPP4B mRNA expression, ( d ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the THP-1 and K562 cells transduced with the plasmids expressing NPM1-wt or NPM1-mA. ( e ) qRT-PCR analysis of INPP4B mRNA expression, ( f ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3, p-Ets-1 and Ets-1 from the OCI-AML3 cells transfected with the control siRNA or siEts-1. ( g ) Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 and SGK3, ( h ) qRT-PCR analysis of INPP4B mRNA expression from the OCI-AML3 cells treated with different concentration of PD98059 (0, 10, 20 and 40 μM). i Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. Proteins were quantified using image software and normalized against β-actin. Data were represented as mean ± s.d. of three individual experiments. * p

Techniques Used: Quantitative RT-PCR, Expressing, Western Blot, Transduction, Transfection, Concentration Assay, Software

NPM1-mA-mediated INPP4B upregulation promotes cell proliferation in OCI-AML3 cells. The NPM1-mA-silenced OCI-AML3 cells were subjected to ( a ) CCK8 assays and ( b ) colony forming assays. c The NPM1-mA-silenced OCI-AML3 cells were transfected with the pEAK-Flag/INPP4B plasmids, western blotting analysis of INPP4B and NPM1-mA. Proteins were quantified using image software and normalized against β-actin. d CCK-8 assay analysis of cell proliferation in NPM1-mA-silenced OCI-AML3 cells, followed by Flag-INPP4B introduction. Data were represented as mean ± s.d. of three individual experiments. * p
Figure Legend Snippet: NPM1-mA-mediated INPP4B upregulation promotes cell proliferation in OCI-AML3 cells. The NPM1-mA-silenced OCI-AML3 cells were subjected to ( a ) CCK8 assays and ( b ) colony forming assays. c The NPM1-mA-silenced OCI-AML3 cells were transfected with the pEAK-Flag/INPP4B plasmids, western blotting analysis of INPP4B and NPM1-mA. Proteins were quantified using image software and normalized against β-actin. d CCK-8 assay analysis of cell proliferation in NPM1-mA-silenced OCI-AML3 cells, followed by Flag-INPP4B introduction. Data were represented as mean ± s.d. of three individual experiments. * p

Techniques Used: Transfection, Western Blot, Software, CCK-8 Assay

High expression of INPP4B is associated with poor survival outcome in NPM1-mutated leukemia. Kaplan-Meier survival data of 153 AML patients were used to analysis ( a ) OS and ( b ) EFS curves according to INPP4B levels. c Heatmap of 38 primary AML samples with NPM1 mutation from TCGA dataset in which INPP4B expression was aligned with patient event (Survival or Death). Kaplan-Meier survival data of patients with NPM1-mutated AML were used to analysis ( d ) OS and ( e ) EFS curves according to INPP4B levels. f Schematic diagram describing the functional significance of INPP4B in the NPM1-mutated leukemia cells. INPP4B promotes leukemia cell survival in a SGK3-dependent and AKT-independent manner. The expression of INPP4B partially upregulated by NPM1-mA is due to ERK/Ets-1 signaling
Figure Legend Snippet: High expression of INPP4B is associated with poor survival outcome in NPM1-mutated leukemia. Kaplan-Meier survival data of 153 AML patients were used to analysis ( a ) OS and ( b ) EFS curves according to INPP4B levels. c Heatmap of 38 primary AML samples with NPM1 mutation from TCGA dataset in which INPP4B expression was aligned with patient event (Survival or Death). Kaplan-Meier survival data of patients with NPM1-mutated AML were used to analysis ( d ) OS and ( e ) EFS curves according to INPP4B levels. f Schematic diagram describing the functional significance of INPP4B in the NPM1-mutated leukemia cells. INPP4B promotes leukemia cell survival in a SGK3-dependent and AKT-independent manner. The expression of INPP4B partially upregulated by NPM1-mA is due to ERK/Ets-1 signaling

Techniques Used: Expressing, Mutagenesis, Functional Assay

High expression levels of INPP4B in leukemia cells with the NPM1 mutation. a RNA-seq mRNA expression data from the TCGA database were used to compare INPP4B expression between AML patients with ( n = 41) and without NPM1 mutation ( n = 130). * p
Figure Legend Snippet: High expression levels of INPP4B in leukemia cells with the NPM1 mutation. a RNA-seq mRNA expression data from the TCGA database were used to compare INPP4B expression between AML patients with ( n = 41) and without NPM1 mutation ( n = 130). * p

Techniques Used: Expressing, Mutagenesis, RNA Sequencing Assay

34) Product Images from "INPP4B promotes cell survival via SGK3 activation in NPM1-mutated leukemia"

Article Title: INPP4B promotes cell survival via SGK3 activation in NPM1-mutated leukemia

Journal: Journal of Experimental & Clinical Cancer Research : CR

doi: 10.1186/s13046-018-0675-9

INPP4B is upregulated by NPM1-mA in leukemia cells via ERK/Ets-1 signaling. ( a ) qRT-PCR analysis of INPP4B mRNA expression, ( b ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. ( c ) qRT-PCR analysis of INPP4B mRNA expression, ( d ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the THP-1 and K562 cells transduced with the plasmids expressing NPM1-wt or NPM1-mA. ( e ) qRT-PCR analysis of INPP4B mRNA expression, ( f ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3, p-Ets-1 and Ets-1 from the OCI-AML3 cells transfected with the control siRNA or siEts-1. ( g ) Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 and SGK3, ( h ) qRT-PCR analysis of INPP4B mRNA expression from the OCI-AML3 cells treated with different concentration of PD98059 (0, 10, 20 and 40 μM). i Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. Proteins were quantified using image software and normalized against β-actin. Data were represented as mean ± s.d. of three individual experiments. * p
Figure Legend Snippet: INPP4B is upregulated by NPM1-mA in leukemia cells via ERK/Ets-1 signaling. ( a ) qRT-PCR analysis of INPP4B mRNA expression, ( b ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. ( c ) qRT-PCR analysis of INPP4B mRNA expression, ( d ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the THP-1 and K562 cells transduced with the plasmids expressing NPM1-wt or NPM1-mA. ( e ) qRT-PCR analysis of INPP4B mRNA expression, ( f ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3, p-Ets-1 and Ets-1 from the OCI-AML3 cells transfected with the control siRNA or siEts-1. ( g ) Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 and SGK3, ( h ) qRT-PCR analysis of INPP4B mRNA expression from the OCI-AML3 cells treated with different concentration of PD98059 (0, 10, 20 and 40 μM). i Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. Proteins were quantified using image software and normalized against β-actin. Data were represented as mean ± s.d. of three individual experiments. * p

Techniques Used: Quantitative RT-PCR, Expressing, Western Blot, Transduction, Transfection, Concentration Assay, Software

NPM1-mA-mediated INPP4B upregulation promotes cell proliferation in OCI-AML3 cells. The NPM1-mA-silenced OCI-AML3 cells were subjected to ( a ) CCK8 assays and ( b ) colony forming assays. c The NPM1-mA-silenced OCI-AML3 cells were transfected with the pEAK-Flag/INPP4B plasmids, western blotting analysis of INPP4B and NPM1-mA. Proteins were quantified using image software and normalized against β-actin. d CCK-8 assay analysis of cell proliferation in NPM1-mA-silenced OCI-AML3 cells, followed by Flag-INPP4B introduction. Data were represented as mean ± s.d. of three individual experiments. * p
Figure Legend Snippet: NPM1-mA-mediated INPP4B upregulation promotes cell proliferation in OCI-AML3 cells. The NPM1-mA-silenced OCI-AML3 cells were subjected to ( a ) CCK8 assays and ( b ) colony forming assays. c The NPM1-mA-silenced OCI-AML3 cells were transfected with the pEAK-Flag/INPP4B plasmids, western blotting analysis of INPP4B and NPM1-mA. Proteins were quantified using image software and normalized against β-actin. d CCK-8 assay analysis of cell proliferation in NPM1-mA-silenced OCI-AML3 cells, followed by Flag-INPP4B introduction. Data were represented as mean ± s.d. of three individual experiments. * p

Techniques Used: Transfection, Western Blot, Software, CCK-8 Assay

High expression of INPP4B is associated with poor survival outcome in NPM1-mutated leukemia. Kaplan-Meier survival data of 153 AML patients were used to analysis ( a ) OS and ( b ) EFS curves according to INPP4B levels. c Heatmap of 38 primary AML samples with NPM1 mutation from TCGA dataset in which INPP4B expression was aligned with patient event (Survival or Death). Kaplan-Meier survival data of patients with NPM1-mutated AML were used to analysis ( d ) OS and ( e ) EFS curves according to INPP4B levels. f Schematic diagram describing the functional significance of INPP4B in the NPM1-mutated leukemia cells. INPP4B promotes leukemia cell survival in a SGK3-dependent and AKT-independent manner. The expression of INPP4B partially upregulated by NPM1-mA is due to ERK/Ets-1 signaling
Figure Legend Snippet: High expression of INPP4B is associated with poor survival outcome in NPM1-mutated leukemia. Kaplan-Meier survival data of 153 AML patients were used to analysis ( a ) OS and ( b ) EFS curves according to INPP4B levels. c Heatmap of 38 primary AML samples with NPM1 mutation from TCGA dataset in which INPP4B expression was aligned with patient event (Survival or Death). Kaplan-Meier survival data of patients with NPM1-mutated AML were used to analysis ( d ) OS and ( e ) EFS curves according to INPP4B levels. f Schematic diagram describing the functional significance of INPP4B in the NPM1-mutated leukemia cells. INPP4B promotes leukemia cell survival in a SGK3-dependent and AKT-independent manner. The expression of INPP4B partially upregulated by NPM1-mA is due to ERK/Ets-1 signaling

Techniques Used: Expressing, Mutagenesis, Functional Assay

High expression levels of INPP4B in leukemia cells with the NPM1 mutation. a RNA-seq mRNA expression data from the TCGA database were used to compare INPP4B expression between AML patients with ( n = 41) and without NPM1 mutation ( n = 130). * p
Figure Legend Snippet: High expression levels of INPP4B in leukemia cells with the NPM1 mutation. a RNA-seq mRNA expression data from the TCGA database were used to compare INPP4B expression between AML patients with ( n = 41) and without NPM1 mutation ( n = 130). * p

Techniques Used: Expressing, Mutagenesis, RNA Sequencing Assay

35) Product Images from "INPP4B promotes cell survival via SGK3 activation in NPM1-mutated leukemia"

Article Title: INPP4B promotes cell survival via SGK3 activation in NPM1-mutated leukemia

Journal: Journal of Experimental & Clinical Cancer Research : CR

doi: 10.1186/s13046-018-0675-9

INPP4B is upregulated by NPM1-mA in leukemia cells via ERK/Ets-1 signaling. ( a ) qRT-PCR analysis of INPP4B mRNA expression, ( b ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. ( c ) qRT-PCR analysis of INPP4B mRNA expression, ( d ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the THP-1 and K562 cells transduced with the plasmids expressing NPM1-wt or NPM1-mA. ( e ) qRT-PCR analysis of INPP4B mRNA expression, ( f ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3, p-Ets-1 and Ets-1 from the OCI-AML3 cells transfected with the control siRNA or siEts-1. ( g ) Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 and SGK3, ( h ) qRT-PCR analysis of INPP4B mRNA expression from the OCI-AML3 cells treated with different concentration of PD98059 (0, 10, 20 and 40 μM). i Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. Proteins were quantified using image software and normalized against β-actin. Data were represented as mean ± s.d. of three individual experiments. * p
Figure Legend Snippet: INPP4B is upregulated by NPM1-mA in leukemia cells via ERK/Ets-1 signaling. ( a ) qRT-PCR analysis of INPP4B mRNA expression, ( b ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. ( c ) qRT-PCR analysis of INPP4B mRNA expression, ( d ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the THP-1 and K562 cells transduced with the plasmids expressing NPM1-wt or NPM1-mA. ( e ) qRT-PCR analysis of INPP4B mRNA expression, ( f ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3, p-Ets-1 and Ets-1 from the OCI-AML3 cells transfected with the control siRNA or siEts-1. ( g ) Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 and SGK3, ( h ) qRT-PCR analysis of INPP4B mRNA expression from the OCI-AML3 cells treated with different concentration of PD98059 (0, 10, 20 and 40 μM). i Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. Proteins were quantified using image software and normalized against β-actin. Data were represented as mean ± s.d. of three individual experiments. * p

Techniques Used: Quantitative RT-PCR, Expressing, Western Blot, Transduction, Transfection, Concentration Assay, Software

NPM1-mA-mediated INPP4B upregulation promotes cell proliferation in OCI-AML3 cells. The NPM1-mA-silenced OCI-AML3 cells were subjected to ( a ) CCK8 assays and ( b ) colony forming assays. c The NPM1-mA-silenced OCI-AML3 cells were transfected with the pEAK-Flag/INPP4B plasmids, western blotting analysis of INPP4B and NPM1-mA. Proteins were quantified using image software and normalized against β-actin. d CCK-8 assay analysis of cell proliferation in NPM1-mA-silenced OCI-AML3 cells, followed by Flag-INPP4B introduction. Data were represented as mean ± s.d. of three individual experiments. * p
Figure Legend Snippet: NPM1-mA-mediated INPP4B upregulation promotes cell proliferation in OCI-AML3 cells. The NPM1-mA-silenced OCI-AML3 cells were subjected to ( a ) CCK8 assays and ( b ) colony forming assays. c The NPM1-mA-silenced OCI-AML3 cells were transfected with the pEAK-Flag/INPP4B plasmids, western blotting analysis of INPP4B and NPM1-mA. Proteins were quantified using image software and normalized against β-actin. d CCK-8 assay analysis of cell proliferation in NPM1-mA-silenced OCI-AML3 cells, followed by Flag-INPP4B introduction. Data were represented as mean ± s.d. of three individual experiments. * p

Techniques Used: Transfection, Western Blot, Software, CCK-8 Assay

High expression of INPP4B is associated with poor survival outcome in NPM1-mutated leukemia. Kaplan-Meier survival data of 153 AML patients were used to analysis ( a ) OS and ( b ) EFS curves according to INPP4B levels. c Heatmap of 38 primary AML samples with NPM1 mutation from TCGA dataset in which INPP4B expression was aligned with patient event (Survival or Death). Kaplan-Meier survival data of patients with NPM1-mutated AML were used to analysis ( d ) OS and ( e ) EFS curves according to INPP4B levels. f Schematic diagram describing the functional significance of INPP4B in the NPM1-mutated leukemia cells. INPP4B promotes leukemia cell survival in a SGK3-dependent and AKT-independent manner. The expression of INPP4B partially upregulated by NPM1-mA is due to ERK/Ets-1 signaling
Figure Legend Snippet: High expression of INPP4B is associated with poor survival outcome in NPM1-mutated leukemia. Kaplan-Meier survival data of 153 AML patients were used to analysis ( a ) OS and ( b ) EFS curves according to INPP4B levels. c Heatmap of 38 primary AML samples with NPM1 mutation from TCGA dataset in which INPP4B expression was aligned with patient event (Survival or Death). Kaplan-Meier survival data of patients with NPM1-mutated AML were used to analysis ( d ) OS and ( e ) EFS curves according to INPP4B levels. f Schematic diagram describing the functional significance of INPP4B in the NPM1-mutated leukemia cells. INPP4B promotes leukemia cell survival in a SGK3-dependent and AKT-independent manner. The expression of INPP4B partially upregulated by NPM1-mA is due to ERK/Ets-1 signaling

Techniques Used: Expressing, Mutagenesis, Functional Assay

High expression levels of INPP4B in leukemia cells with the NPM1 mutation. a RNA-seq mRNA expression data from the TCGA database were used to compare INPP4B expression between AML patients with ( n = 41) and without NPM1 mutation ( n = 130). * p
Figure Legend Snippet: High expression levels of INPP4B in leukemia cells with the NPM1 mutation. a RNA-seq mRNA expression data from the TCGA database were used to compare INPP4B expression between AML patients with ( n = 41) and without NPM1 mutation ( n = 130). * p

Techniques Used: Expressing, Mutagenesis, RNA Sequencing Assay

Related Articles

Incubation:

Article Title: INPP4B promotes cell survival via SGK3 activation in NPM1-mutated leukemia
Article Snippet: .. The membranes were blocked in 5% low-fat dry milk for 3 h, and then incubated overnight at 4 °C with primary antibodies against INPP4B, p-SGK3T320 , SGK3, p-AKTT308 , AKT, p-ERK, ERK (Cell Signaling Technology, MA, USA); p-Ets-1, Ets-1, Flag (Bioworld Technology Inc. MN, USA); NPM1-mA (Abcam, Cambrige, UK) and β-actin (Santa Cruz Biotechnology Inc. CA, USA) as loading control. .. Membranes were washed in Tris-buffered saline (TBS) (10 mM Tris-HCl pH 8, 150 mM NaCl) containing 0.1% Tween 20, and then incubated with HRP-conjugated secondary antibody for 1 h, and subsequently exposed to enhanced chemiluminescence substrate (Millipore, MA, USA).

Over Expression:

Article Title: INPP4B promotes cell survival via SGK3 activation in NPM1-mutated leukemia
Article Snippet: .. This hypothesis was further confirmed, as we found that INPP4B mRNA levels were increased upon ectopic overexpression of NPM1-mA in THP-1 and K562 leukemia cell lines (Fig. ). .. Moreover, INPP4B and phosphorylated SGK3 proteins were consistently elevated, due to the introduction of NPM1-mA (Fig. ).

other:

Article Title: INPP4B promotes cell survival via SGK3 activation in NPM1-mutated leukemia
Article Snippet: More notably, the loss of NPM1-mA weakened ERK/Ets-1 signaling and then reduced INPP4B protein levels.

Article Title: INPP4B promotes cell survival via SGK3 activation in NPM1-mutated leukemia
Article Snippet: INPP4B is upregulated by NPM1-mA via ERK/Ets-1 signaling in leukemia cells We evaluated the potential molecular mechanism underlying INPP4B upregulation in leukemia cells with NPM1-mA.

Expressing:

Article Title: INPP4B promotes cell survival via SGK3 activation in NPM1-mutated leukemia
Article Snippet: .. Given that NPM1-mA enhanced INPP4B expression, we then validated the impact of NPM1-mA on the proliferation of leukemia cells. .. The results showed that knockdown of NPM1-mA significantly attenuated cell proliferation in OCI-AML3 cells, as compared to the control group (Fig. ).

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    Abcam npm1 ma
    INPP4B is upregulated by <t>NPM1-mA</t> in leukemia cells via ERK/Ets-1 signaling. ( a ) qRT-PCR analysis of INPP4B mRNA expression, ( b ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. ( c ) qRT-PCR analysis of INPP4B mRNA expression, ( d ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the THP-1 and K562 cells transduced with the plasmids expressing NPM1-wt or NPM1-mA. ( e ) qRT-PCR analysis of INPP4B mRNA expression, ( f ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3, p-Ets-1 and Ets-1 from the OCI-AML3 cells transfected with the control siRNA or siEts-1. ( g ) Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 and SGK3, ( h ) qRT-PCR analysis of INPP4B mRNA expression from the OCI-AML3 cells treated with different concentration of PD98059 (0, 10, 20 and 40 μM). i Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. Proteins were quantified using image software and normalized against β-actin. Data were represented as mean ± s.d. of three individual experiments. * p
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    INPP4B is upregulated by NPM1-mA in leukemia cells via ERK/Ets-1 signaling. ( a ) qRT-PCR analysis of INPP4B mRNA expression, ( b ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. ( c ) qRT-PCR analysis of INPP4B mRNA expression, ( d ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the THP-1 and K562 cells transduced with the plasmids expressing NPM1-wt or NPM1-mA. ( e ) qRT-PCR analysis of INPP4B mRNA expression, ( f ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3, p-Ets-1 and Ets-1 from the OCI-AML3 cells transfected with the control siRNA or siEts-1. ( g ) Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 and SGK3, ( h ) qRT-PCR analysis of INPP4B mRNA expression from the OCI-AML3 cells treated with different concentration of PD98059 (0, 10, 20 and 40 μM). i Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. Proteins were quantified using image software and normalized against β-actin. Data were represented as mean ± s.d. of three individual experiments. * p

    Journal: Journal of Experimental & Clinical Cancer Research : CR

    Article Title: INPP4B promotes cell survival via SGK3 activation in NPM1-mutated leukemia

    doi: 10.1186/s13046-018-0675-9

    Figure Lengend Snippet: INPP4B is upregulated by NPM1-mA in leukemia cells via ERK/Ets-1 signaling. ( a ) qRT-PCR analysis of INPP4B mRNA expression, ( b ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. ( c ) qRT-PCR analysis of INPP4B mRNA expression, ( d ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the THP-1 and K562 cells transduced with the plasmids expressing NPM1-wt or NPM1-mA. ( e ) qRT-PCR analysis of INPP4B mRNA expression, ( f ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3, p-Ets-1 and Ets-1 from the OCI-AML3 cells transfected with the control siRNA or siEts-1. ( g ) Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 and SGK3, ( h ) qRT-PCR analysis of INPP4B mRNA expression from the OCI-AML3 cells treated with different concentration of PD98059 (0, 10, 20 and 40 μM). i Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. Proteins were quantified using image software and normalized against β-actin. Data were represented as mean ± s.d. of three individual experiments. * p

    Article Snippet: Moreover, our previous study has verified that ERK signaling is continuously activated by NPM1-mA [ ].

    Techniques: Quantitative RT-PCR, Expressing, Western Blot, Transduction, Transfection, Concentration Assay, Software

    NPM1-mA-mediated INPP4B upregulation promotes cell proliferation in OCI-AML3 cells. The NPM1-mA-silenced OCI-AML3 cells were subjected to ( a ) CCK8 assays and ( b ) colony forming assays. c The NPM1-mA-silenced OCI-AML3 cells were transfected with the pEAK-Flag/INPP4B plasmids, western blotting analysis of INPP4B and NPM1-mA. Proteins were quantified using image software and normalized against β-actin. d CCK-8 assay analysis of cell proliferation in NPM1-mA-silenced OCI-AML3 cells, followed by Flag-INPP4B introduction. Data were represented as mean ± s.d. of three individual experiments. * p

    Journal: Journal of Experimental & Clinical Cancer Research : CR

    Article Title: INPP4B promotes cell survival via SGK3 activation in NPM1-mutated leukemia

    doi: 10.1186/s13046-018-0675-9

    Figure Lengend Snippet: NPM1-mA-mediated INPP4B upregulation promotes cell proliferation in OCI-AML3 cells. The NPM1-mA-silenced OCI-AML3 cells were subjected to ( a ) CCK8 assays and ( b ) colony forming assays. c The NPM1-mA-silenced OCI-AML3 cells were transfected with the pEAK-Flag/INPP4B plasmids, western blotting analysis of INPP4B and NPM1-mA. Proteins were quantified using image software and normalized against β-actin. d CCK-8 assay analysis of cell proliferation in NPM1-mA-silenced OCI-AML3 cells, followed by Flag-INPP4B introduction. Data were represented as mean ± s.d. of three individual experiments. * p

    Article Snippet: Moreover, our previous study has verified that ERK signaling is continuously activated by NPM1-mA [ ].

    Techniques: Transfection, Western Blot, Software, CCK-8 Assay

    High expression of INPP4B is associated with poor survival outcome in NPM1-mutated leukemia. Kaplan-Meier survival data of 153 AML patients were used to analysis ( a ) OS and ( b ) EFS curves according to INPP4B levels. c Heatmap of 38 primary AML samples with NPM1 mutation from TCGA dataset in which INPP4B expression was aligned with patient event (Survival or Death). Kaplan-Meier survival data of patients with NPM1-mutated AML were used to analysis ( d ) OS and ( e ) EFS curves according to INPP4B levels. f Schematic diagram describing the functional significance of INPP4B in the NPM1-mutated leukemia cells. INPP4B promotes leukemia cell survival in a SGK3-dependent and AKT-independent manner. The expression of INPP4B partially upregulated by NPM1-mA is due to ERK/Ets-1 signaling

    Journal: Journal of Experimental & Clinical Cancer Research : CR

    Article Title: INPP4B promotes cell survival via SGK3 activation in NPM1-mutated leukemia

    doi: 10.1186/s13046-018-0675-9

    Figure Lengend Snippet: High expression of INPP4B is associated with poor survival outcome in NPM1-mutated leukemia. Kaplan-Meier survival data of 153 AML patients were used to analysis ( a ) OS and ( b ) EFS curves according to INPP4B levels. c Heatmap of 38 primary AML samples with NPM1 mutation from TCGA dataset in which INPP4B expression was aligned with patient event (Survival or Death). Kaplan-Meier survival data of patients with NPM1-mutated AML were used to analysis ( d ) OS and ( e ) EFS curves according to INPP4B levels. f Schematic diagram describing the functional significance of INPP4B in the NPM1-mutated leukemia cells. INPP4B promotes leukemia cell survival in a SGK3-dependent and AKT-independent manner. The expression of INPP4B partially upregulated by NPM1-mA is due to ERK/Ets-1 signaling

    Article Snippet: Moreover, our previous study has verified that ERK signaling is continuously activated by NPM1-mA [ ].

    Techniques: Expressing, Mutagenesis, Functional Assay

    High expression levels of INPP4B in leukemia cells with the NPM1 mutation. a RNA-seq mRNA expression data from the TCGA database were used to compare INPP4B expression between AML patients with ( n = 41) and without NPM1 mutation ( n = 130). * p

    Journal: Journal of Experimental & Clinical Cancer Research : CR

    Article Title: INPP4B promotes cell survival via SGK3 activation in NPM1-mutated leukemia

    doi: 10.1186/s13046-018-0675-9

    Figure Lengend Snippet: High expression levels of INPP4B in leukemia cells with the NPM1 mutation. a RNA-seq mRNA expression data from the TCGA database were used to compare INPP4B expression between AML patients with ( n = 41) and without NPM1 mutation ( n = 130). * p

    Article Snippet: Moreover, our previous study has verified that ERK signaling is continuously activated by NPM1-mA [ ].

    Techniques: Expressing, Mutagenesis, RNA Sequencing Assay

    INPP4B is upregulated by NPM1-mA in leukemia cells via ERK/Ets-1 signaling. ( a ) qRT-PCR analysis of INPP4B mRNA expression, ( b ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. ( c ) qRT-PCR analysis of INPP4B mRNA expression, ( d ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the THP-1 and K562 cells transduced with the plasmids expressing NPM1-wt or NPM1-mA. ( e ) qRT-PCR analysis of INPP4B mRNA expression, ( f ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3, p-Ets-1 and Ets-1 from the OCI-AML3 cells transfected with the control siRNA or siEts-1. ( g ) Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 and SGK3, ( h ) qRT-PCR analysis of INPP4B mRNA expression from the OCI-AML3 cells treated with different concentration of PD98059 (0, 10, 20 and 40 μM). i Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. Proteins were quantified using image software and normalized against β-actin. Data were represented as mean ± s.d. of three individual experiments. * p

    Journal: Journal of Experimental & Clinical Cancer Research : CR

    Article Title: INPP4B promotes cell survival via SGK3 activation in NPM1-mutated leukemia

    doi: 10.1186/s13046-018-0675-9

    Figure Lengend Snippet: INPP4B is upregulated by NPM1-mA in leukemia cells via ERK/Ets-1 signaling. ( a ) qRT-PCR analysis of INPP4B mRNA expression, ( b ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. ( c ) qRT-PCR analysis of INPP4B mRNA expression, ( d ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the THP-1 and K562 cells transduced with the plasmids expressing NPM1-wt or NPM1-mA. ( e ) qRT-PCR analysis of INPP4B mRNA expression, ( f ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3, p-Ets-1 and Ets-1 from the OCI-AML3 cells transfected with the control siRNA or siEts-1. ( g ) Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 and SGK3, ( h ) qRT-PCR analysis of INPP4B mRNA expression from the OCI-AML3 cells treated with different concentration of PD98059 (0, 10, 20 and 40 μM). i Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. Proteins were quantified using image software and normalized against β-actin. Data were represented as mean ± s.d. of three individual experiments. * p

    Article Snippet: This hypothesis was further confirmed, as we found that INPP4B mRNA levels were increased upon ectopic overexpression of NPM1-mA in THP-1 and K562 leukemia cell lines (Fig. ).

    Techniques: Quantitative RT-PCR, Expressing, Western Blot, Transduction, Transfection, Concentration Assay, Software

    NPM1-mA-mediated INPP4B upregulation promotes cell proliferation in OCI-AML3 cells. The NPM1-mA-silenced OCI-AML3 cells were subjected to ( a ) CCK8 assays and ( b ) colony forming assays. c The NPM1-mA-silenced OCI-AML3 cells were transfected with the pEAK-Flag/INPP4B plasmids, western blotting analysis of INPP4B and NPM1-mA. Proteins were quantified using image software and normalized against β-actin. d CCK-8 assay analysis of cell proliferation in NPM1-mA-silenced OCI-AML3 cells, followed by Flag-INPP4B introduction. Data were represented as mean ± s.d. of three individual experiments. * p

    Journal: Journal of Experimental & Clinical Cancer Research : CR

    Article Title: INPP4B promotes cell survival via SGK3 activation in NPM1-mutated leukemia

    doi: 10.1186/s13046-018-0675-9

    Figure Lengend Snippet: NPM1-mA-mediated INPP4B upregulation promotes cell proliferation in OCI-AML3 cells. The NPM1-mA-silenced OCI-AML3 cells were subjected to ( a ) CCK8 assays and ( b ) colony forming assays. c The NPM1-mA-silenced OCI-AML3 cells were transfected with the pEAK-Flag/INPP4B plasmids, western blotting analysis of INPP4B and NPM1-mA. Proteins were quantified using image software and normalized against β-actin. d CCK-8 assay analysis of cell proliferation in NPM1-mA-silenced OCI-AML3 cells, followed by Flag-INPP4B introduction. Data were represented as mean ± s.d. of three individual experiments. * p

    Article Snippet: This hypothesis was further confirmed, as we found that INPP4B mRNA levels were increased upon ectopic overexpression of NPM1-mA in THP-1 and K562 leukemia cell lines (Fig. ).

    Techniques: Transfection, Western Blot, Software, CCK-8 Assay

    High expression of INPP4B is associated with poor survival outcome in NPM1-mutated leukemia. Kaplan-Meier survival data of 153 AML patients were used to analysis ( a ) OS and ( b ) EFS curves according to INPP4B levels. c Heatmap of 38 primary AML samples with NPM1 mutation from TCGA dataset in which INPP4B expression was aligned with patient event (Survival or Death). Kaplan-Meier survival data of patients with NPM1-mutated AML were used to analysis ( d ) OS and ( e ) EFS curves according to INPP4B levels. f Schematic diagram describing the functional significance of INPP4B in the NPM1-mutated leukemia cells. INPP4B promotes leukemia cell survival in a SGK3-dependent and AKT-independent manner. The expression of INPP4B partially upregulated by NPM1-mA is due to ERK/Ets-1 signaling

    Journal: Journal of Experimental & Clinical Cancer Research : CR

    Article Title: INPP4B promotes cell survival via SGK3 activation in NPM1-mutated leukemia

    doi: 10.1186/s13046-018-0675-9

    Figure Lengend Snippet: High expression of INPP4B is associated with poor survival outcome in NPM1-mutated leukemia. Kaplan-Meier survival data of 153 AML patients were used to analysis ( a ) OS and ( b ) EFS curves according to INPP4B levels. c Heatmap of 38 primary AML samples with NPM1 mutation from TCGA dataset in which INPP4B expression was aligned with patient event (Survival or Death). Kaplan-Meier survival data of patients with NPM1-mutated AML were used to analysis ( d ) OS and ( e ) EFS curves according to INPP4B levels. f Schematic diagram describing the functional significance of INPP4B in the NPM1-mutated leukemia cells. INPP4B promotes leukemia cell survival in a SGK3-dependent and AKT-independent manner. The expression of INPP4B partially upregulated by NPM1-mA is due to ERK/Ets-1 signaling

    Article Snippet: This hypothesis was further confirmed, as we found that INPP4B mRNA levels were increased upon ectopic overexpression of NPM1-mA in THP-1 and K562 leukemia cell lines (Fig. ).

    Techniques: Expressing, Mutagenesis, Functional Assay

    High expression levels of INPP4B in leukemia cells with the NPM1 mutation. a RNA-seq mRNA expression data from the TCGA database were used to compare INPP4B expression between AML patients with ( n = 41) and without NPM1 mutation ( n = 130). * p

    Journal: Journal of Experimental & Clinical Cancer Research : CR

    Article Title: INPP4B promotes cell survival via SGK3 activation in NPM1-mutated leukemia

    doi: 10.1186/s13046-018-0675-9

    Figure Lengend Snippet: High expression levels of INPP4B in leukemia cells with the NPM1 mutation. a RNA-seq mRNA expression data from the TCGA database were used to compare INPP4B expression between AML patients with ( n = 41) and without NPM1 mutation ( n = 130). * p

    Article Snippet: This hypothesis was further confirmed, as we found that INPP4B mRNA levels were increased upon ectopic overexpression of NPM1-mA in THP-1 and K562 leukemia cell lines (Fig. ).

    Techniques: Expressing, Mutagenesis, RNA Sequencing Assay