Structured Review

Proteintech anti nox2
Western Blot Analysis of Vascular Function and Stress Responses : This figure presents the outcomes of Western blot analysis, offering detailed insights into the protein expression levels of pivotal molecular components implicated in vascular function and stress responses. The cumulative data presented enable meticulous comparative assessments of protein levels across distinct experimental groups, thereby facilitating the exploration of potential variations in vascular signaling pathways and stress-mediated mechanisms. This figure represents the results of Western blot analysis and Cumulative data for phosphorylated endothelial nitric oxide synthase (P-eNOS), NADPH oxidase 1 (Nox1), <t>NADPH</t> <t>oxidase</t> <t>2</t> <t>(Nox2),</t> along with endoplasmic reticulum stress markers Activating Transcription Factor 6 (ATF6), Binding Immunoglobulin Protein (BIP), and CCAAT/enhancer-binding protein (CHOP) in mesenteric resistance arteries (MRA) isolated from the male (A, B, C) and female mice (D, E, F) across all experimental groups (n = 5), Statistical analysis involved the application of One-way ANOVA followed by Tukey's post hoc test was applied for A-F. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 for comparisons between C57BL/6J vs 2K1C vs 2K1C + anti-PDCA-1groups.
Anti Nox2, supplied by Proteintech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti nox2/product/Proteintech
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
anti nox2 - by Bioz Stars, 2024-06
86/100 stars

Images

1) Product Images from "Role of plasmacytoid dendritic cells in vascular dysfunction in mice with renovascular hypertension"

Article Title: Role of plasmacytoid dendritic cells in vascular dysfunction in mice with renovascular hypertension

Journal: Heliyon

doi: 10.1016/j.heliyon.2024.e31799

Western Blot Analysis of Vascular Function and Stress Responses : This figure presents the outcomes of Western blot analysis, offering detailed insights into the protein expression levels of pivotal molecular components implicated in vascular function and stress responses. The cumulative data presented enable meticulous comparative assessments of protein levels across distinct experimental groups, thereby facilitating the exploration of potential variations in vascular signaling pathways and stress-mediated mechanisms. This figure represents the results of Western blot analysis and Cumulative data for phosphorylated endothelial nitric oxide synthase (P-eNOS), NADPH oxidase 1 (Nox1), NADPH oxidase 2 (Nox2), along with endoplasmic reticulum stress markers Activating Transcription Factor 6 (ATF6), Binding Immunoglobulin Protein (BIP), and CCAAT/enhancer-binding protein (CHOP) in mesenteric resistance arteries (MRA) isolated from the male (A, B, C) and female mice (D, E, F) across all experimental groups (n = 5), Statistical analysis involved the application of One-way ANOVA followed by Tukey's post hoc test was applied for A-F. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 for comparisons between C57BL/6J vs 2K1C vs 2K1C + anti-PDCA-1groups.
Figure Legend Snippet: Western Blot Analysis of Vascular Function and Stress Responses : This figure presents the outcomes of Western blot analysis, offering detailed insights into the protein expression levels of pivotal molecular components implicated in vascular function and stress responses. The cumulative data presented enable meticulous comparative assessments of protein levels across distinct experimental groups, thereby facilitating the exploration of potential variations in vascular signaling pathways and stress-mediated mechanisms. This figure represents the results of Western blot analysis and Cumulative data for phosphorylated endothelial nitric oxide synthase (P-eNOS), NADPH oxidase 1 (Nox1), NADPH oxidase 2 (Nox2), along with endoplasmic reticulum stress markers Activating Transcription Factor 6 (ATF6), Binding Immunoglobulin Protein (BIP), and CCAAT/enhancer-binding protein (CHOP) in mesenteric resistance arteries (MRA) isolated from the male (A, B, C) and female mice (D, E, F) across all experimental groups (n = 5), Statistical analysis involved the application of One-way ANOVA followed by Tukey's post hoc test was applied for A-F. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 for comparisons between C57BL/6J vs 2K1C vs 2K1C + anti-PDCA-1groups.

Techniques Used: Western Blot, Expressing, Binding Assay, Isolation


Structured Review

Proteintech anti nox2
Anti Nox2, supplied by Proteintech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti nox2/product/Proteintech
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
anti nox2 - by Bioz Stars, 2024-06
86/100 stars

Images


Structured Review

Proteintech nox2
Sc improves the oxidative stress, ferroptosis, and apoptosis-related protein effects induced by THP in the myocardium of SD rats. (A) Western blots. Semi-quantitative analysis of the protein expression of (B) <t>NOX2,</t> (C) NOX4, (D) NRF2, (E) GPX4, (F) Bax/Bcl-2, (G) cleaved caspase 3/total caspase 3 and (H) cleaved caspase 9/total-caspase 9 in each group. Values are expressed as the means ± standard deviation. **P<0.01 and ***P<0.001 vs. ND. # P<0.05 and ## P<0.01 vs. THP. Sc, scutellarein; THP, pirarubicin; NOX2, NADPH oxidase 2; NOX4, NADPH oxidase 4; NRF2, erythroid 2-related factor 2; GPX4, glutathione peroxidase 4; ND, normal diet; Dex, dexrazoxane.
Nox2, supplied by Proteintech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/nox2/product/Proteintech
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
nox2 - by Bioz Stars, 2024-06
86/100 stars

Images

1) Product Images from "Food therapy of scutellarein ameliorates pirarubicin‑induced cardiotoxicity in rats by inhibiting apoptosis and ferroptosis through regulation of NOX2‑induced oxidative stress"

Article Title: Food therapy of scutellarein ameliorates pirarubicin‑induced cardiotoxicity in rats by inhibiting apoptosis and ferroptosis through regulation of NOX2‑induced oxidative stress

Journal: Molecular Medicine Reports

doi: 10.3892/mmr.2024.13208

Sc improves the oxidative stress, ferroptosis, and apoptosis-related protein effects induced by THP in the myocardium of SD rats. (A) Western blots. Semi-quantitative analysis of the protein expression of (B) NOX2, (C) NOX4, (D) NRF2, (E) GPX4, (F) Bax/Bcl-2, (G) cleaved caspase 3/total caspase 3 and (H) cleaved caspase 9/total-caspase 9 in each group. Values are expressed as the means ± standard deviation. **P<0.01 and ***P<0.001 vs. ND. # P<0.05 and ## P<0.01 vs. THP. Sc, scutellarein; THP, pirarubicin; NOX2, NADPH oxidase 2; NOX4, NADPH oxidase 4; NRF2, erythroid 2-related factor 2; GPX4, glutathione peroxidase 4; ND, normal diet; Dex, dexrazoxane.
Figure Legend Snippet: Sc improves the oxidative stress, ferroptosis, and apoptosis-related protein effects induced by THP in the myocardium of SD rats. (A) Western blots. Semi-quantitative analysis of the protein expression of (B) NOX2, (C) NOX4, (D) NRF2, (E) GPX4, (F) Bax/Bcl-2, (G) cleaved caspase 3/total caspase 3 and (H) cleaved caspase 9/total-caspase 9 in each group. Values are expressed as the means ± standard deviation. **P<0.01 and ***P<0.001 vs. ND. # P<0.05 and ## P<0.01 vs. THP. Sc, scutellarein; THP, pirarubicin; NOX2, NADPH oxidase 2; NOX4, NADPH oxidase 4; NRF2, erythroid 2-related factor 2; GPX4, glutathione peroxidase 4; ND, normal diet; Dex, dexrazoxane.

Techniques Used: Western Blot, Expressing, Standard Deviation

Effects of Sc, THP, GSK, Fer-1, and erastin on the apoptosis of H9c2 cardiomyocytes and the expression of NOX2. (A) TUNEL assay. (B) Semi-quantitative analysis of the average fluorescence intensity of the results of the TUNEL assay. (C) Immunofluorescence staining of NOX2. (D) Semi-quantitative analysis of the average fluorescence intensity of NOX2. Values are expressed as means ± standard deviation. **P<0.01 vs. CON. ## P<0.01 vs. THP. ^^ P<0.01 vs. erastin. Sc, scutellarein; THP, pirarubicin; GSK, GSK2795039; Fer-1, ferrostatin-1; NOX2, NADPH oxidase 2; CON, control.
Figure Legend Snippet: Effects of Sc, THP, GSK, Fer-1, and erastin on the apoptosis of H9c2 cardiomyocytes and the expression of NOX2. (A) TUNEL assay. (B) Semi-quantitative analysis of the average fluorescence intensity of the results of the TUNEL assay. (C) Immunofluorescence staining of NOX2. (D) Semi-quantitative analysis of the average fluorescence intensity of NOX2. Values are expressed as means ± standard deviation. **P<0.01 vs. CON. ## P<0.01 vs. THP. ^^ P<0.01 vs. erastin. Sc, scutellarein; THP, pirarubicin; GSK, GSK2795039; Fer-1, ferrostatin-1; NOX2, NADPH oxidase 2; CON, control.

Techniques Used: Expressing, TUNEL Assay, Fluorescence, Immunofluorescence, Staining, Standard Deviation

Effects of Sc, THP, GSK, Fer-1, and erastin on oxidative stress, ferroptosis, and apoptosis-related proteins in H9c2 cells. (A and B) Western blots. (C and D) Semi-quantitative analysis of the protein expression of GPX4, NOX2, Bax/Bcl-2, cleaved caspase 3/total caspase 3 and cleaved caspase 9/total caspase 9 in each group. Values are expressed as the means ± standard deviation. **P<0.01, ***P<0.001 and ****P<0.0001 vs. CON. # P<0.05 and ## P<0.01 vs. THP. ^ P<0.05 and ^^ P<0.01 vs. erastin. Sc, scutellarein; THP, pirarubicin; GSK, GSK2795039; Fer-1, ferrostatin-1; GPX4, glutathione peroxidase 4; NOX2, NADPH oxidase 2.
Figure Legend Snippet: Effects of Sc, THP, GSK, Fer-1, and erastin on oxidative stress, ferroptosis, and apoptosis-related proteins in H9c2 cells. (A and B) Western blots. (C and D) Semi-quantitative analysis of the protein expression of GPX4, NOX2, Bax/Bcl-2, cleaved caspase 3/total caspase 3 and cleaved caspase 9/total caspase 9 in each group. Values are expressed as the means ± standard deviation. **P<0.01, ***P<0.001 and ****P<0.0001 vs. CON. # P<0.05 and ## P<0.01 vs. THP. ^ P<0.05 and ^^ P<0.01 vs. erastin. Sc, scutellarein; THP, pirarubicin; GSK, GSK2795039; Fer-1, ferrostatin-1; GPX4, glutathione peroxidase 4; NOX2, NADPH oxidase 2.

Techniques Used: Western Blot, Expressing, Standard Deviation

Sc inhibits cell apoptosis and ferroptosis by negatively regulating the oxidative stress axis (NOX2-ROS), thereby improving the cardiotoxicity induced by pirarubicin. NOX2, NADPH oxidase 2; ROS, reactive oxygen species; THP, pirarubicin; NOX4, NADPH oxidase 4; NRF2, erythroid 2-related factor 2; GSH, glutathione; GPX4, glutathione peroxidase 4.
Figure Legend Snippet: Sc inhibits cell apoptosis and ferroptosis by negatively regulating the oxidative stress axis (NOX2-ROS), thereby improving the cardiotoxicity induced by pirarubicin. NOX2, NADPH oxidase 2; ROS, reactive oxygen species; THP, pirarubicin; NOX4, NADPH oxidase 4; NRF2, erythroid 2-related factor 2; GSH, glutathione; GPX4, glutathione peroxidase 4.

Techniques Used:


Structured Review

Proteintech antibodies against nox2
Sc improves the oxidative stress, ferroptosis, and apoptosis-related protein effects induced by THP in the myocardium of SD rats. (A) Western blots. Semi-quantitative analysis of the protein expression of (B) <t>NOX2,</t> (C) NOX4, (D) NRF2, (E) GPX4, (F) Bax/Bcl-2, (G) cleaved caspase 3/total caspase 3 and (H) cleaved caspase 9/total-caspase 9 in each group. Values are expressed as the means ± standard deviation. **P<0.01 and ***P<0.001 vs. ND. # P<0.05 and ## P<0.01 vs. THP. Sc, scutellarein; THP, pirarubicin; NOX2, NADPH oxidase 2; NOX4, NADPH oxidase 4; NRF2, erythroid 2-related factor 2; GPX4, glutathione peroxidase 4; ND, normal diet; Dex, dexrazoxane.
Antibodies Against Nox2, supplied by Proteintech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/antibodies against nox2/product/Proteintech
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
antibodies against nox2 - by Bioz Stars, 2024-06
86/100 stars

Images

1) Product Images from "Food therapy of scutellarein ameliorates pirarubicin‑induced cardiotoxicity in rats by inhibiting apoptosis and ferroptosis through regulation of NOX2‑induced oxidative stress"

Article Title: Food therapy of scutellarein ameliorates pirarubicin‑induced cardiotoxicity in rats by inhibiting apoptosis and ferroptosis through regulation of NOX2‑induced oxidative stress

Journal: Molecular Medicine Reports

doi: 10.3892/mmr.2024.13208

Sc improves the oxidative stress, ferroptosis, and apoptosis-related protein effects induced by THP in the myocardium of SD rats. (A) Western blots. Semi-quantitative analysis of the protein expression of (B) NOX2, (C) NOX4, (D) NRF2, (E) GPX4, (F) Bax/Bcl-2, (G) cleaved caspase 3/total caspase 3 and (H) cleaved caspase 9/total-caspase 9 in each group. Values are expressed as the means ± standard deviation. **P<0.01 and ***P<0.001 vs. ND. # P<0.05 and ## P<0.01 vs. THP. Sc, scutellarein; THP, pirarubicin; NOX2, NADPH oxidase 2; NOX4, NADPH oxidase 4; NRF2, erythroid 2-related factor 2; GPX4, glutathione peroxidase 4; ND, normal diet; Dex, dexrazoxane.
Figure Legend Snippet: Sc improves the oxidative stress, ferroptosis, and apoptosis-related protein effects induced by THP in the myocardium of SD rats. (A) Western blots. Semi-quantitative analysis of the protein expression of (B) NOX2, (C) NOX4, (D) NRF2, (E) GPX4, (F) Bax/Bcl-2, (G) cleaved caspase 3/total caspase 3 and (H) cleaved caspase 9/total-caspase 9 in each group. Values are expressed as the means ± standard deviation. **P<0.01 and ***P<0.001 vs. ND. # P<0.05 and ## P<0.01 vs. THP. Sc, scutellarein; THP, pirarubicin; NOX2, NADPH oxidase 2; NOX4, NADPH oxidase 4; NRF2, erythroid 2-related factor 2; GPX4, glutathione peroxidase 4; ND, normal diet; Dex, dexrazoxane.

Techniques Used: Western Blot, Expressing, Standard Deviation

Effects of Sc, THP, GSK, Fer-1, and erastin on the apoptosis of H9c2 cardiomyocytes and the expression of NOX2. (A) TUNEL assay. (B) Semi-quantitative analysis of the average fluorescence intensity of the results of the TUNEL assay. (C) Immunofluorescence staining of NOX2. (D) Semi-quantitative analysis of the average fluorescence intensity of NOX2. Values are expressed as means ± standard deviation. **P<0.01 vs. CON. ## P<0.01 vs. THP. ^^ P<0.01 vs. erastin. Sc, scutellarein; THP, pirarubicin; GSK, GSK2795039; Fer-1, ferrostatin-1; NOX2, NADPH oxidase 2; CON, control.
Figure Legend Snippet: Effects of Sc, THP, GSK, Fer-1, and erastin on the apoptosis of H9c2 cardiomyocytes and the expression of NOX2. (A) TUNEL assay. (B) Semi-quantitative analysis of the average fluorescence intensity of the results of the TUNEL assay. (C) Immunofluorescence staining of NOX2. (D) Semi-quantitative analysis of the average fluorescence intensity of NOX2. Values are expressed as means ± standard deviation. **P<0.01 vs. CON. ## P<0.01 vs. THP. ^^ P<0.01 vs. erastin. Sc, scutellarein; THP, pirarubicin; GSK, GSK2795039; Fer-1, ferrostatin-1; NOX2, NADPH oxidase 2; CON, control.

Techniques Used: Expressing, TUNEL Assay, Fluorescence, Immunofluorescence, Staining, Standard Deviation

Effects of Sc, THP, GSK, Fer-1, and erastin on oxidative stress, ferroptosis, and apoptosis-related proteins in H9c2 cells. (A and B) Western blots. (C and D) Semi-quantitative analysis of the protein expression of GPX4, NOX2, Bax/Bcl-2, cleaved caspase 3/total caspase 3 and cleaved caspase 9/total caspase 9 in each group. Values are expressed as the means ± standard deviation. **P<0.01, ***P<0.001 and ****P<0.0001 vs. CON. # P<0.05 and ## P<0.01 vs. THP. ^ P<0.05 and ^^ P<0.01 vs. erastin. Sc, scutellarein; THP, pirarubicin; GSK, GSK2795039; Fer-1, ferrostatin-1; GPX4, glutathione peroxidase 4; NOX2, NADPH oxidase 2.
Figure Legend Snippet: Effects of Sc, THP, GSK, Fer-1, and erastin on oxidative stress, ferroptosis, and apoptosis-related proteins in H9c2 cells. (A and B) Western blots. (C and D) Semi-quantitative analysis of the protein expression of GPX4, NOX2, Bax/Bcl-2, cleaved caspase 3/total caspase 3 and cleaved caspase 9/total caspase 9 in each group. Values are expressed as the means ± standard deviation. **P<0.01, ***P<0.001 and ****P<0.0001 vs. CON. # P<0.05 and ## P<0.01 vs. THP. ^ P<0.05 and ^^ P<0.01 vs. erastin. Sc, scutellarein; THP, pirarubicin; GSK, GSK2795039; Fer-1, ferrostatin-1; GPX4, glutathione peroxidase 4; NOX2, NADPH oxidase 2.

Techniques Used: Western Blot, Expressing, Standard Deviation

Sc inhibits cell apoptosis and ferroptosis by negatively regulating the oxidative stress axis (NOX2-ROS), thereby improving the cardiotoxicity induced by pirarubicin. NOX2, NADPH oxidase 2; ROS, reactive oxygen species; THP, pirarubicin; NOX4, NADPH oxidase 4; NRF2, erythroid 2-related factor 2; GSH, glutathione; GPX4, glutathione peroxidase 4.
Figure Legend Snippet: Sc inhibits cell apoptosis and ferroptosis by negatively regulating the oxidative stress axis (NOX2-ROS), thereby improving the cardiotoxicity induced by pirarubicin. NOX2, NADPH oxidase 2; ROS, reactive oxygen species; THP, pirarubicin; NOX4, NADPH oxidase 4; NRF2, erythroid 2-related factor 2; GSH, glutathione; GPX4, glutathione peroxidase 4.

Techniques Used:


Structured Review

Proteintech antibody nox2
Antibody Nox2, supplied by Proteintech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/antibody nox2/product/Proteintech
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
antibody nox2 - by Bioz Stars, 2024-06
86/100 stars

Images


Structured Review

Proteintech antibody nox2
Antibody Nox2, supplied by Proteintech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/antibody nox2/product/Proteintech
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
antibody nox2 - by Bioz Stars, 2024-06
86/100 stars

Images

nicotinamide adenine dinucleotide phosphate oxidase 2 nox2 polyclonal antibody  (Proteintech)

 
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    Structured Review

    Proteintech nicotinamide adenine dinucleotide phosphate oxidase 2 nox2 polyclonal antibody
    Probucol alleviates hippocampal insulin resistance and differentially regulates radical species in cerebral cortex of mice. (A–F) Western blot analysis of lysates of cerebral cortex and hippocampus from male mice with or without probucol administration for 12 weeks. The levels of proteins were quantified in the left ( n = 4 mice per group). Cortex iNOS, ordinary one-way ANOVA ( p = 0.0296), followed by Tukey’s multiple comparisons test. Cortex <t>NOX2,</t> ordinary one-way ANOVA ( p = 0.0099), followed by Tukey’s multiple comparisons test. Hippocampal iNOS, ordinary one-way ANOVA ( p = 0.0025), followed by Tukey’s multiple comparisons test. Hippocampal NOX2, ordinary one-way ANOVA ( p = 0.2109). (G–L) Western blot analysis of AKT phosphorylation and PSD95 in the brain extracts of male mice with or without probucol administration for 5 weeks ( n = 4 mice per group). Cortex p-AKT, ordinary one-way ANOVA ( p = 0.9565). Cortex PSD95, ordinary one-way ANOVA ( p = 0.0547). Hippocampus p-AKT, ordinary one-way ANOVA ( p = 0.0179), followed by Tukey’s multiple comparisons test. Hippocampus PSD95, ordinary one-way ANOVA ( p = 0.0435), followed by Tukey’s multiple comparisons test. p < 0.05 was indicative of statistical significance.
    Nicotinamide Adenine Dinucleotide Phosphate Oxidase 2 Nox2 Polyclonal Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nicotinamide adenine dinucleotide phosphate oxidase 2 nox2 polyclonal antibody/product/Proteintech
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    nicotinamide adenine dinucleotide phosphate oxidase 2 nox2 polyclonal antibody - by Bioz Stars, 2024-06
    86/100 stars

    Images

    1) Product Images from "Probucol mitigates high-fat diet-induced cognitive and social impairments by regulating brain redox and insulin resistance"

    Article Title: Probucol mitigates high-fat diet-induced cognitive and social impairments by regulating brain redox and insulin resistance

    Journal: Frontiers in Neuroscience

    doi: 10.3389/fnins.2024.1368552

    Probucol alleviates hippocampal insulin resistance and differentially regulates radical species in cerebral cortex of mice. (A–F) Western blot analysis of lysates of cerebral cortex and hippocampus from male mice with or without probucol administration for 12 weeks. The levels of proteins were quantified in the left ( n = 4 mice per group). Cortex iNOS, ordinary one-way ANOVA ( p = 0.0296), followed by Tukey’s multiple comparisons test. Cortex NOX2, ordinary one-way ANOVA ( p = 0.0099), followed by Tukey’s multiple comparisons test. Hippocampal iNOS, ordinary one-way ANOVA ( p = 0.0025), followed by Tukey’s multiple comparisons test. Hippocampal NOX2, ordinary one-way ANOVA ( p = 0.2109). (G–L) Western blot analysis of AKT phosphorylation and PSD95 in the brain extracts of male mice with or without probucol administration for 5 weeks ( n = 4 mice per group). Cortex p-AKT, ordinary one-way ANOVA ( p = 0.9565). Cortex PSD95, ordinary one-way ANOVA ( p = 0.0547). Hippocampus p-AKT, ordinary one-way ANOVA ( p = 0.0179), followed by Tukey’s multiple comparisons test. Hippocampus PSD95, ordinary one-way ANOVA ( p = 0.0435), followed by Tukey’s multiple comparisons test. p < 0.05 was indicative of statistical significance.
    Figure Legend Snippet: Probucol alleviates hippocampal insulin resistance and differentially regulates radical species in cerebral cortex of mice. (A–F) Western blot analysis of lysates of cerebral cortex and hippocampus from male mice with or without probucol administration for 12 weeks. The levels of proteins were quantified in the left ( n = 4 mice per group). Cortex iNOS, ordinary one-way ANOVA ( p = 0.0296), followed by Tukey’s multiple comparisons test. Cortex NOX2, ordinary one-way ANOVA ( p = 0.0099), followed by Tukey’s multiple comparisons test. Hippocampal iNOS, ordinary one-way ANOVA ( p = 0.0025), followed by Tukey’s multiple comparisons test. Hippocampal NOX2, ordinary one-way ANOVA ( p = 0.2109). (G–L) Western blot analysis of AKT phosphorylation and PSD95 in the brain extracts of male mice with or without probucol administration for 5 weeks ( n = 4 mice per group). Cortex p-AKT, ordinary one-way ANOVA ( p = 0.9565). Cortex PSD95, ordinary one-way ANOVA ( p = 0.0547). Hippocampus p-AKT, ordinary one-way ANOVA ( p = 0.0179), followed by Tukey’s multiple comparisons test. Hippocampus PSD95, ordinary one-way ANOVA ( p = 0.0435), followed by Tukey’s multiple comparisons test. p < 0.05 was indicative of statistical significance.

    Techniques Used: Western Blot


    Structured Review

    Proteintech nox2 polyclonal antibody
    Analysis of catalytic subunit <t>NOX2</t> of NADPH oxidase in U-937 cells (A) and THP-1 cells (B) . Control (a) and proteins isolated from EVs co-cultured with U-937 cells (c). In b and d, U-937 and THP-1 cells were differentiated using 250 nM PMA and 150 nM PMA, respectively, in the absence and presence of EVs. In lanes e and f, differentiation was induced using 5 μM in the absence and presence of EVs, respectively, while in lanes f and h, 10 μM ascorbic acid was used. The densitometric quantification of NOX2 expression is presented in the lower panel within the figure.
    Nox2 Polyclonal Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nox2 polyclonal antibody/product/Proteintech
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    nox2 polyclonal antibody - by Bioz Stars, 2024-06
    86/100 stars

    Images

    1) Product Images from "NOX2 and NOX4 expression in monocytes and macrophages-extracellular vesicles in signalling and therapeutics"

    Article Title: NOX2 and NOX4 expression in monocytes and macrophages-extracellular vesicles in signalling and therapeutics

    Journal: Frontiers in Cell and Developmental Biology

    doi: 10.3389/fcell.2024.1342227

    Analysis of catalytic subunit NOX2 of NADPH oxidase in U-937 cells (A) and THP-1 cells (B) . Control (a) and proteins isolated from EVs co-cultured with U-937 cells (c). In b and d, U-937 and THP-1 cells were differentiated using 250 nM PMA and 150 nM PMA, respectively, in the absence and presence of EVs. In lanes e and f, differentiation was induced using 5 μM in the absence and presence of EVs, respectively, while in lanes f and h, 10 μM ascorbic acid was used. The densitometric quantification of NOX2 expression is presented in the lower panel within the figure.
    Figure Legend Snippet: Analysis of catalytic subunit NOX2 of NADPH oxidase in U-937 cells (A) and THP-1 cells (B) . Control (a) and proteins isolated from EVs co-cultured with U-937 cells (c). In b and d, U-937 and THP-1 cells were differentiated using 250 nM PMA and 150 nM PMA, respectively, in the absence and presence of EVs. In lanes e and f, differentiation was induced using 5 μM in the absence and presence of EVs, respectively, while in lanes f and h, 10 μM ascorbic acid was used. The densitometric quantification of NOX2 expression is presented in the lower panel within the figure.

    Techniques Used: Isolation, Cell Culture, Expressing


    Structured Review

    Proteintech nox2
    Nox2, supplied by Proteintech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nox2/product/Proteintech
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    nox2 - by Bioz Stars, 2024-06
    86/100 stars

    Images


    Structured Review

    Proteintech nox2
    Dapagliflozin attenuated cardiomyocyte oxidative stress <i>in vivo</i>. (A) Target proteins were examined by Western blotting. Representative protein blotting results are shown in figure. (B)Statistical analysis of NRF2/β-Actin, NOX4/β-Actin, <t>NOX2/β-Actin,</t> NQO1/β-Actin, SOD2/β-Actin (n = 6). Comparisons were performed using one-way ANOVA for multiple groups. (C) The expression and location of iNOS were determined by immunohistochemistry. (D) Statistical analysis of iNOS area (n = 6). Comparisons were performed using one-way ANOVA for multiple groups. (E) The expression and location of ROS were determined by immunofluorescence. (F) Statistical analysis of fluorescence intensity (n = 6). Data are presented as the mean ± SEM. Comparisons were performed using one-way ANOVA for multiple groups or Student's t-test for two groups. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.
    Nox2, supplied by Proteintech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nox2/product/Proteintech
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    nox2 - by Bioz Stars, 2024-06
    86/100 stars

    Images

    1) Product Images from "Dapagliflozin ameliorates myocardial infarction injury through AMPKα-dependent regulation of oxidative stress and apoptosis"

    Article Title: Dapagliflozin ameliorates myocardial infarction injury through AMPKα-dependent regulation of oxidative stress and apoptosis

    Journal: Heliyon

    doi: 10.1016/j.heliyon.2024.e29160

    Dapagliflozin attenuated cardiomyocyte oxidative stress <i>in vivo</i>. (A) Target proteins were examined by Western blotting. Representative protein blotting results are shown in figure. (B)Statistical analysis of NRF2/β-Actin, NOX4/β-Actin, NOX2/β-Actin, NQO1/β-Actin, SOD2/β-Actin (n = 6). Comparisons were performed using one-way ANOVA for multiple groups. (C) The expression and location of iNOS were determined by immunohistochemistry. (D) Statistical analysis of iNOS area (n = 6). Comparisons were performed using one-way ANOVA for multiple groups. (E) The expression and location of ROS were determined by immunofluorescence. (F) Statistical analysis of fluorescence intensity (n = 6). Data are presented as the mean ± SEM. Comparisons were performed using one-way ANOVA for multiple groups or Student's t-test for two groups. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.
    Figure Legend Snippet: Dapagliflozin attenuated cardiomyocyte oxidative stress in vivo. (A) Target proteins were examined by Western blotting. Representative protein blotting results are shown in figure. (B)Statistical analysis of NRF2/β-Actin, NOX4/β-Actin, NOX2/β-Actin, NQO1/β-Actin, SOD2/β-Actin (n = 6). Comparisons were performed using one-way ANOVA for multiple groups. (C) The expression and location of iNOS were determined by immunohistochemistry. (D) Statistical analysis of iNOS area (n = 6). Comparisons were performed using one-way ANOVA for multiple groups. (E) The expression and location of ROS were determined by immunofluorescence. (F) Statistical analysis of fluorescence intensity (n = 6). Data are presented as the mean ± SEM. Comparisons were performed using one-way ANOVA for multiple groups or Student's t-test for two groups. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.

    Techniques Used: In Vivo, Western Blot, Expressing, Immunohistochemistry, Immunofluorescence, Fluorescence

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  • 86
    Proteintech anti nox2
    Western Blot Analysis of Vascular Function and Stress Responses : This figure presents the outcomes of Western blot analysis, offering detailed insights into the protein expression levels of pivotal molecular components implicated in vascular function and stress responses. The cumulative data presented enable meticulous comparative assessments of protein levels across distinct experimental groups, thereby facilitating the exploration of potential variations in vascular signaling pathways and stress-mediated mechanisms. This figure represents the results of Western blot analysis and Cumulative data for phosphorylated endothelial nitric oxide synthase (P-eNOS), NADPH oxidase 1 (Nox1), <t>NADPH</t> <t>oxidase</t> <t>2</t> <t>(Nox2),</t> along with endoplasmic reticulum stress markers Activating Transcription Factor 6 (ATF6), Binding Immunoglobulin Protein (BIP), and CCAAT/enhancer-binding protein (CHOP) in mesenteric resistance arteries (MRA) isolated from the male (A, B, C) and female mice (D, E, F) across all experimental groups (n = 5), Statistical analysis involved the application of One-way ANOVA followed by Tukey's post hoc test was applied for A-F. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 for comparisons between C57BL/6J vs 2K1C vs 2K1C + anti-PDCA-1groups.
    Anti Nox2, supplied by Proteintech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Proteintech nox2
    Sc improves the oxidative stress, ferroptosis, and apoptosis-related protein effects induced by THP in the myocardium of SD rats. (A) Western blots. Semi-quantitative analysis of the protein expression of (B) <t>NOX2,</t> (C) NOX4, (D) NRF2, (E) GPX4, (F) Bax/Bcl-2, (G) cleaved caspase 3/total caspase 3 and (H) cleaved caspase 9/total-caspase 9 in each group. Values are expressed as the means ± standard deviation. **P<0.01 and ***P<0.001 vs. ND. # P<0.05 and ## P<0.01 vs. THP. Sc, scutellarein; THP, pirarubicin; NOX2, NADPH oxidase 2; NOX4, NADPH oxidase 4; NRF2, erythroid 2-related factor 2; GPX4, glutathione peroxidase 4; ND, normal diet; Dex, dexrazoxane.
    Nox2, supplied by Proteintech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Proteintech antibodies against nox2
    Sc improves the oxidative stress, ferroptosis, and apoptosis-related protein effects induced by THP in the myocardium of SD rats. (A) Western blots. Semi-quantitative analysis of the protein expression of (B) <t>NOX2,</t> (C) NOX4, (D) NRF2, (E) GPX4, (F) Bax/Bcl-2, (G) cleaved caspase 3/total caspase 3 and (H) cleaved caspase 9/total-caspase 9 in each group. Values are expressed as the means ± standard deviation. **P<0.01 and ***P<0.001 vs. ND. # P<0.05 and ## P<0.01 vs. THP. Sc, scutellarein; THP, pirarubicin; NOX2, NADPH oxidase 2; NOX4, NADPH oxidase 4; NRF2, erythroid 2-related factor 2; GPX4, glutathione peroxidase 4; ND, normal diet; Dex, dexrazoxane.
    Antibodies Against Nox2, supplied by Proteintech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Proteintech antibody nox2
    Sc improves the oxidative stress, ferroptosis, and apoptosis-related protein effects induced by THP in the myocardium of SD rats. (A) Western blots. Semi-quantitative analysis of the protein expression of (B) <t>NOX2,</t> (C) NOX4, (D) NRF2, (E) GPX4, (F) Bax/Bcl-2, (G) cleaved caspase 3/total caspase 3 and (H) cleaved caspase 9/total-caspase 9 in each group. Values are expressed as the means ± standard deviation. **P<0.01 and ***P<0.001 vs. ND. # P<0.05 and ## P<0.01 vs. THP. Sc, scutellarein; THP, pirarubicin; NOX2, NADPH oxidase 2; NOX4, NADPH oxidase 4; NRF2, erythroid 2-related factor 2; GPX4, glutathione peroxidase 4; ND, normal diet; Dex, dexrazoxane.
    Antibody Nox2, supplied by Proteintech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Proteintech nicotinamide adenine dinucleotide phosphate oxidase 2 nox2 polyclonal antibody
    Probucol alleviates hippocampal insulin resistance and differentially regulates radical species in cerebral cortex of mice. (A–F) Western blot analysis of lysates of cerebral cortex and hippocampus from male mice with or without probucol administration for 12 weeks. The levels of proteins were quantified in the left ( n = 4 mice per group). Cortex iNOS, ordinary one-way ANOVA ( p = 0.0296), followed by Tukey’s multiple comparisons test. Cortex <t>NOX2,</t> ordinary one-way ANOVA ( p = 0.0099), followed by Tukey’s multiple comparisons test. Hippocampal iNOS, ordinary one-way ANOVA ( p = 0.0025), followed by Tukey’s multiple comparisons test. Hippocampal NOX2, ordinary one-way ANOVA ( p = 0.2109). (G–L) Western blot analysis of AKT phosphorylation and PSD95 in the brain extracts of male mice with or without probucol administration for 5 weeks ( n = 4 mice per group). Cortex p-AKT, ordinary one-way ANOVA ( p = 0.9565). Cortex PSD95, ordinary one-way ANOVA ( p = 0.0547). Hippocampus p-AKT, ordinary one-way ANOVA ( p = 0.0179), followed by Tukey’s multiple comparisons test. Hippocampus PSD95, ordinary one-way ANOVA ( p = 0.0435), followed by Tukey’s multiple comparisons test. p < 0.05 was indicative of statistical significance.
    Nicotinamide Adenine Dinucleotide Phosphate Oxidase 2 Nox2 Polyclonal Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Proteintech nox2 polyclonal antibody
    Analysis of catalytic subunit <t>NOX2</t> of NADPH oxidase in U-937 cells (A) and THP-1 cells (B) . Control (a) and proteins isolated from EVs co-cultured with U-937 cells (c). In b and d, U-937 and THP-1 cells were differentiated using 250 nM PMA and 150 nM PMA, respectively, in the absence and presence of EVs. In lanes e and f, differentiation was induced using 5 μM in the absence and presence of EVs, respectively, while in lanes f and h, 10 μM ascorbic acid was used. The densitometric quantification of NOX2 expression is presented in the lower panel within the figure.
    Nox2 Polyclonal Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Western Blot Analysis of Vascular Function and Stress Responses : This figure presents the outcomes of Western blot analysis, offering detailed insights into the protein expression levels of pivotal molecular components implicated in vascular function and stress responses. The cumulative data presented enable meticulous comparative assessments of protein levels across distinct experimental groups, thereby facilitating the exploration of potential variations in vascular signaling pathways and stress-mediated mechanisms. This figure represents the results of Western blot analysis and Cumulative data for phosphorylated endothelial nitric oxide synthase (P-eNOS), NADPH oxidase 1 (Nox1), NADPH oxidase 2 (Nox2), along with endoplasmic reticulum stress markers Activating Transcription Factor 6 (ATF6), Binding Immunoglobulin Protein (BIP), and CCAAT/enhancer-binding protein (CHOP) in mesenteric resistance arteries (MRA) isolated from the male (A, B, C) and female mice (D, E, F) across all experimental groups (n = 5), Statistical analysis involved the application of One-way ANOVA followed by Tukey's post hoc test was applied for A-F. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 for comparisons between C57BL/6J vs 2K1C vs 2K1C + anti-PDCA-1groups.

    Journal: Heliyon

    Article Title: Role of plasmacytoid dendritic cells in vascular dysfunction in mice with renovascular hypertension

    doi: 10.1016/j.heliyon.2024.e31799

    Figure Lengend Snippet: Western Blot Analysis of Vascular Function and Stress Responses : This figure presents the outcomes of Western blot analysis, offering detailed insights into the protein expression levels of pivotal molecular components implicated in vascular function and stress responses. The cumulative data presented enable meticulous comparative assessments of protein levels across distinct experimental groups, thereby facilitating the exploration of potential variations in vascular signaling pathways and stress-mediated mechanisms. This figure represents the results of Western blot analysis and Cumulative data for phosphorylated endothelial nitric oxide synthase (P-eNOS), NADPH oxidase 1 (Nox1), NADPH oxidase 2 (Nox2), along with endoplasmic reticulum stress markers Activating Transcription Factor 6 (ATF6), Binding Immunoglobulin Protein (BIP), and CCAAT/enhancer-binding protein (CHOP) in mesenteric resistance arteries (MRA) isolated from the male (A, B, C) and female mice (D, E, F) across all experimental groups (n = 5), Statistical analysis involved the application of One-way ANOVA followed by Tukey's post hoc test was applied for A-F. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 for comparisons between C57BL/6J vs 2K1C vs 2K1C + anti-PDCA-1groups.

    Article Snippet: We used specific antibodies against Anti-Bip (C50B12, Cell Signaling, #3177), Anti-CHOP (Cell Signaling, #2895), iNOS (Proteintech, # 18985-1-AP), eNOS (Cell Signalling, D9A5L Rabbit mAb # 32027), Phospho-eNOS (Ser 1177, Cell Signalling (C9C3) Rabbit mAb 9570)), Anti-Cox2 ((Cayman, # 160126), Anti-NOX1 (Novus, #NBP131546), Anti-NOX2 (Proteintech, #19013-1-AP), Anti-ATF-6 (Abcam, #Ab37149), and beta-actin (Santa Cruz #SC47778).

    Techniques: Western Blot, Expressing, Binding Assay, Isolation

    Sc improves the oxidative stress, ferroptosis, and apoptosis-related protein effects induced by THP in the myocardium of SD rats. (A) Western blots. Semi-quantitative analysis of the protein expression of (B) NOX2, (C) NOX4, (D) NRF2, (E) GPX4, (F) Bax/Bcl-2, (G) cleaved caspase 3/total caspase 3 and (H) cleaved caspase 9/total-caspase 9 in each group. Values are expressed as the means ± standard deviation. **P<0.01 and ***P<0.001 vs. ND. # P<0.05 and ## P<0.01 vs. THP. Sc, scutellarein; THP, pirarubicin; NOX2, NADPH oxidase 2; NOX4, NADPH oxidase 4; NRF2, erythroid 2-related factor 2; GPX4, glutathione peroxidase 4; ND, normal diet; Dex, dexrazoxane.

    Journal: Molecular Medicine Reports

    Article Title: Food therapy of scutellarein ameliorates pirarubicin‑induced cardiotoxicity in rats by inhibiting apoptosis and ferroptosis through regulation of NOX2‑induced oxidative stress

    doi: 10.3892/mmr.2024.13208

    Figure Lengend Snippet: Sc improves the oxidative stress, ferroptosis, and apoptosis-related protein effects induced by THP in the myocardium of SD rats. (A) Western blots. Semi-quantitative analysis of the protein expression of (B) NOX2, (C) NOX4, (D) NRF2, (E) GPX4, (F) Bax/Bcl-2, (G) cleaved caspase 3/total caspase 3 and (H) cleaved caspase 9/total-caspase 9 in each group. Values are expressed as the means ± standard deviation. **P<0.01 and ***P<0.001 vs. ND. # P<0.05 and ## P<0.01 vs. THP. Sc, scutellarein; THP, pirarubicin; NOX2, NADPH oxidase 2; NOX4, NADPH oxidase 4; NRF2, erythroid 2-related factor 2; GPX4, glutathione peroxidase 4; ND, normal diet; Dex, dexrazoxane.

    Article Snippet: An iron assay kit (, cat. no. ab83366) was purchased from Abcam, while the antibodies against glutathione peroxidase 4 (GPX4), NOX2, NOX4, erythroid 2-related factor 2 (NRF2), Bax, Bcl-2, GAPDH, caspase 3 and caspase 9 from Proteintech Group, Inc.

    Techniques: Western Blot, Expressing, Standard Deviation

    Effects of Sc, THP, GSK, Fer-1, and erastin on the apoptosis of H9c2 cardiomyocytes and the expression of NOX2. (A) TUNEL assay. (B) Semi-quantitative analysis of the average fluorescence intensity of the results of the TUNEL assay. (C) Immunofluorescence staining of NOX2. (D) Semi-quantitative analysis of the average fluorescence intensity of NOX2. Values are expressed as means ± standard deviation. **P<0.01 vs. CON. ## P<0.01 vs. THP. ^^ P<0.01 vs. erastin. Sc, scutellarein; THP, pirarubicin; GSK, GSK2795039; Fer-1, ferrostatin-1; NOX2, NADPH oxidase 2; CON, control.

    Journal: Molecular Medicine Reports

    Article Title: Food therapy of scutellarein ameliorates pirarubicin‑induced cardiotoxicity in rats by inhibiting apoptosis and ferroptosis through regulation of NOX2‑induced oxidative stress

    doi: 10.3892/mmr.2024.13208

    Figure Lengend Snippet: Effects of Sc, THP, GSK, Fer-1, and erastin on the apoptosis of H9c2 cardiomyocytes and the expression of NOX2. (A) TUNEL assay. (B) Semi-quantitative analysis of the average fluorescence intensity of the results of the TUNEL assay. (C) Immunofluorescence staining of NOX2. (D) Semi-quantitative analysis of the average fluorescence intensity of NOX2. Values are expressed as means ± standard deviation. **P<0.01 vs. CON. ## P<0.01 vs. THP. ^^ P<0.01 vs. erastin. Sc, scutellarein; THP, pirarubicin; GSK, GSK2795039; Fer-1, ferrostatin-1; NOX2, NADPH oxidase 2; CON, control.

    Article Snippet: An iron assay kit (, cat. no. ab83366) was purchased from Abcam, while the antibodies against glutathione peroxidase 4 (GPX4), NOX2, NOX4, erythroid 2-related factor 2 (NRF2), Bax, Bcl-2, GAPDH, caspase 3 and caspase 9 from Proteintech Group, Inc.

    Techniques: Expressing, TUNEL Assay, Fluorescence, Immunofluorescence, Staining, Standard Deviation

    Effects of Sc, THP, GSK, Fer-1, and erastin on oxidative stress, ferroptosis, and apoptosis-related proteins in H9c2 cells. (A and B) Western blots. (C and D) Semi-quantitative analysis of the protein expression of GPX4, NOX2, Bax/Bcl-2, cleaved caspase 3/total caspase 3 and cleaved caspase 9/total caspase 9 in each group. Values are expressed as the means ± standard deviation. **P<0.01, ***P<0.001 and ****P<0.0001 vs. CON. # P<0.05 and ## P<0.01 vs. THP. ^ P<0.05 and ^^ P<0.01 vs. erastin. Sc, scutellarein; THP, pirarubicin; GSK, GSK2795039; Fer-1, ferrostatin-1; GPX4, glutathione peroxidase 4; NOX2, NADPH oxidase 2.

    Journal: Molecular Medicine Reports

    Article Title: Food therapy of scutellarein ameliorates pirarubicin‑induced cardiotoxicity in rats by inhibiting apoptosis and ferroptosis through regulation of NOX2‑induced oxidative stress

    doi: 10.3892/mmr.2024.13208

    Figure Lengend Snippet: Effects of Sc, THP, GSK, Fer-1, and erastin on oxidative stress, ferroptosis, and apoptosis-related proteins in H9c2 cells. (A and B) Western blots. (C and D) Semi-quantitative analysis of the protein expression of GPX4, NOX2, Bax/Bcl-2, cleaved caspase 3/total caspase 3 and cleaved caspase 9/total caspase 9 in each group. Values are expressed as the means ± standard deviation. **P<0.01, ***P<0.001 and ****P<0.0001 vs. CON. # P<0.05 and ## P<0.01 vs. THP. ^ P<0.05 and ^^ P<0.01 vs. erastin. Sc, scutellarein; THP, pirarubicin; GSK, GSK2795039; Fer-1, ferrostatin-1; GPX4, glutathione peroxidase 4; NOX2, NADPH oxidase 2.

    Article Snippet: An iron assay kit (, cat. no. ab83366) was purchased from Abcam, while the antibodies against glutathione peroxidase 4 (GPX4), NOX2, NOX4, erythroid 2-related factor 2 (NRF2), Bax, Bcl-2, GAPDH, caspase 3 and caspase 9 from Proteintech Group, Inc.

    Techniques: Western Blot, Expressing, Standard Deviation

    Sc inhibits cell apoptosis and ferroptosis by negatively regulating the oxidative stress axis (NOX2-ROS), thereby improving the cardiotoxicity induced by pirarubicin. NOX2, NADPH oxidase 2; ROS, reactive oxygen species; THP, pirarubicin; NOX4, NADPH oxidase 4; NRF2, erythroid 2-related factor 2; GSH, glutathione; GPX4, glutathione peroxidase 4.

    Journal: Molecular Medicine Reports

    Article Title: Food therapy of scutellarein ameliorates pirarubicin‑induced cardiotoxicity in rats by inhibiting apoptosis and ferroptosis through regulation of NOX2‑induced oxidative stress

    doi: 10.3892/mmr.2024.13208

    Figure Lengend Snippet: Sc inhibits cell apoptosis and ferroptosis by negatively regulating the oxidative stress axis (NOX2-ROS), thereby improving the cardiotoxicity induced by pirarubicin. NOX2, NADPH oxidase 2; ROS, reactive oxygen species; THP, pirarubicin; NOX4, NADPH oxidase 4; NRF2, erythroid 2-related factor 2; GSH, glutathione; GPX4, glutathione peroxidase 4.

    Article Snippet: An iron assay kit (, cat. no. ab83366) was purchased from Abcam, while the antibodies against glutathione peroxidase 4 (GPX4), NOX2, NOX4, erythroid 2-related factor 2 (NRF2), Bax, Bcl-2, GAPDH, caspase 3 and caspase 9 from Proteintech Group, Inc.

    Techniques:

    Sc improves the oxidative stress, ferroptosis, and apoptosis-related protein effects induced by THP in the myocardium of SD rats. (A) Western blots. Semi-quantitative analysis of the protein expression of (B) NOX2, (C) NOX4, (D) NRF2, (E) GPX4, (F) Bax/Bcl-2, (G) cleaved caspase 3/total caspase 3 and (H) cleaved caspase 9/total-caspase 9 in each group. Values are expressed as the means ± standard deviation. **P<0.01 and ***P<0.001 vs. ND. # P<0.05 and ## P<0.01 vs. THP. Sc, scutellarein; THP, pirarubicin; NOX2, NADPH oxidase 2; NOX4, NADPH oxidase 4; NRF2, erythroid 2-related factor 2; GPX4, glutathione peroxidase 4; ND, normal diet; Dex, dexrazoxane.

    Journal: Molecular Medicine Reports

    Article Title: Food therapy of scutellarein ameliorates pirarubicin‑induced cardiotoxicity in rats by inhibiting apoptosis and ferroptosis through regulation of NOX2‑induced oxidative stress

    doi: 10.3892/mmr.2024.13208

    Figure Lengend Snippet: Sc improves the oxidative stress, ferroptosis, and apoptosis-related protein effects induced by THP in the myocardium of SD rats. (A) Western blots. Semi-quantitative analysis of the protein expression of (B) NOX2, (C) NOX4, (D) NRF2, (E) GPX4, (F) Bax/Bcl-2, (G) cleaved caspase 3/total caspase 3 and (H) cleaved caspase 9/total-caspase 9 in each group. Values are expressed as the means ± standard deviation. **P<0.01 and ***P<0.001 vs. ND. # P<0.05 and ## P<0.01 vs. THP. Sc, scutellarein; THP, pirarubicin; NOX2, NADPH oxidase 2; NOX4, NADPH oxidase 4; NRF2, erythroid 2-related factor 2; GPX4, glutathione peroxidase 4; ND, normal diet; Dex, dexrazoxane.

    Article Snippet: Following blocking with goat serum (cat. no. C0265; Beyotime Institute of Biotechnology) at room temperature for 30 min, the cells were first incubated at 4°C, overnight with primary antibodies against NOX2 (cat. no. 19013-1-AP; 1:200; Proteintech Group, Inc.) and then with the corresponding secondary antibody [FITC-labeled goat anti-rabbit IgG (H+L); cat. no. A0562; 1:500; Beyotime Institute of Biotechnology] at room temperature for 90 min.

    Techniques: Western Blot, Expressing, Standard Deviation

    Effects of Sc, THP, GSK, Fer-1, and erastin on the apoptosis of H9c2 cardiomyocytes and the expression of NOX2. (A) TUNEL assay. (B) Semi-quantitative analysis of the average fluorescence intensity of the results of the TUNEL assay. (C) Immunofluorescence staining of NOX2. (D) Semi-quantitative analysis of the average fluorescence intensity of NOX2. Values are expressed as means ± standard deviation. **P<0.01 vs. CON. ## P<0.01 vs. THP. ^^ P<0.01 vs. erastin. Sc, scutellarein; THP, pirarubicin; GSK, GSK2795039; Fer-1, ferrostatin-1; NOX2, NADPH oxidase 2; CON, control.

    Journal: Molecular Medicine Reports

    Article Title: Food therapy of scutellarein ameliorates pirarubicin‑induced cardiotoxicity in rats by inhibiting apoptosis and ferroptosis through regulation of NOX2‑induced oxidative stress

    doi: 10.3892/mmr.2024.13208

    Figure Lengend Snippet: Effects of Sc, THP, GSK, Fer-1, and erastin on the apoptosis of H9c2 cardiomyocytes and the expression of NOX2. (A) TUNEL assay. (B) Semi-quantitative analysis of the average fluorescence intensity of the results of the TUNEL assay. (C) Immunofluorescence staining of NOX2. (D) Semi-quantitative analysis of the average fluorescence intensity of NOX2. Values are expressed as means ± standard deviation. **P<0.01 vs. CON. ## P<0.01 vs. THP. ^^ P<0.01 vs. erastin. Sc, scutellarein; THP, pirarubicin; GSK, GSK2795039; Fer-1, ferrostatin-1; NOX2, NADPH oxidase 2; CON, control.

    Article Snippet: Following blocking with goat serum (cat. no. C0265; Beyotime Institute of Biotechnology) at room temperature for 30 min, the cells were first incubated at 4°C, overnight with primary antibodies against NOX2 (cat. no. 19013-1-AP; 1:200; Proteintech Group, Inc.) and then with the corresponding secondary antibody [FITC-labeled goat anti-rabbit IgG (H+L); cat. no. A0562; 1:500; Beyotime Institute of Biotechnology] at room temperature for 90 min.

    Techniques: Expressing, TUNEL Assay, Fluorescence, Immunofluorescence, Staining, Standard Deviation

    Effects of Sc, THP, GSK, Fer-1, and erastin on oxidative stress, ferroptosis, and apoptosis-related proteins in H9c2 cells. (A and B) Western blots. (C and D) Semi-quantitative analysis of the protein expression of GPX4, NOX2, Bax/Bcl-2, cleaved caspase 3/total caspase 3 and cleaved caspase 9/total caspase 9 in each group. Values are expressed as the means ± standard deviation. **P<0.01, ***P<0.001 and ****P<0.0001 vs. CON. # P<0.05 and ## P<0.01 vs. THP. ^ P<0.05 and ^^ P<0.01 vs. erastin. Sc, scutellarein; THP, pirarubicin; GSK, GSK2795039; Fer-1, ferrostatin-1; GPX4, glutathione peroxidase 4; NOX2, NADPH oxidase 2.

    Journal: Molecular Medicine Reports

    Article Title: Food therapy of scutellarein ameliorates pirarubicin‑induced cardiotoxicity in rats by inhibiting apoptosis and ferroptosis through regulation of NOX2‑induced oxidative stress

    doi: 10.3892/mmr.2024.13208

    Figure Lengend Snippet: Effects of Sc, THP, GSK, Fer-1, and erastin on oxidative stress, ferroptosis, and apoptosis-related proteins in H9c2 cells. (A and B) Western blots. (C and D) Semi-quantitative analysis of the protein expression of GPX4, NOX2, Bax/Bcl-2, cleaved caspase 3/total caspase 3 and cleaved caspase 9/total caspase 9 in each group. Values are expressed as the means ± standard deviation. **P<0.01, ***P<0.001 and ****P<0.0001 vs. CON. # P<0.05 and ## P<0.01 vs. THP. ^ P<0.05 and ^^ P<0.01 vs. erastin. Sc, scutellarein; THP, pirarubicin; GSK, GSK2795039; Fer-1, ferrostatin-1; GPX4, glutathione peroxidase 4; NOX2, NADPH oxidase 2.

    Article Snippet: Following blocking with goat serum (cat. no. C0265; Beyotime Institute of Biotechnology) at room temperature for 30 min, the cells were first incubated at 4°C, overnight with primary antibodies against NOX2 (cat. no. 19013-1-AP; 1:200; Proteintech Group, Inc.) and then with the corresponding secondary antibody [FITC-labeled goat anti-rabbit IgG (H+L); cat. no. A0562; 1:500; Beyotime Institute of Biotechnology] at room temperature for 90 min.

    Techniques: Western Blot, Expressing, Standard Deviation

    Sc inhibits cell apoptosis and ferroptosis by negatively regulating the oxidative stress axis (NOX2-ROS), thereby improving the cardiotoxicity induced by pirarubicin. NOX2, NADPH oxidase 2; ROS, reactive oxygen species; THP, pirarubicin; NOX4, NADPH oxidase 4; NRF2, erythroid 2-related factor 2; GSH, glutathione; GPX4, glutathione peroxidase 4.

    Journal: Molecular Medicine Reports

    Article Title: Food therapy of scutellarein ameliorates pirarubicin‑induced cardiotoxicity in rats by inhibiting apoptosis and ferroptosis through regulation of NOX2‑induced oxidative stress

    doi: 10.3892/mmr.2024.13208

    Figure Lengend Snippet: Sc inhibits cell apoptosis and ferroptosis by negatively regulating the oxidative stress axis (NOX2-ROS), thereby improving the cardiotoxicity induced by pirarubicin. NOX2, NADPH oxidase 2; ROS, reactive oxygen species; THP, pirarubicin; NOX4, NADPH oxidase 4; NRF2, erythroid 2-related factor 2; GSH, glutathione; GPX4, glutathione peroxidase 4.

    Article Snippet: Following blocking with goat serum (cat. no. C0265; Beyotime Institute of Biotechnology) at room temperature for 30 min, the cells were first incubated at 4°C, overnight with primary antibodies against NOX2 (cat. no. 19013-1-AP; 1:200; Proteintech Group, Inc.) and then with the corresponding secondary antibody [FITC-labeled goat anti-rabbit IgG (H+L); cat. no. A0562; 1:500; Beyotime Institute of Biotechnology] at room temperature for 90 min.

    Techniques:

    Probucol alleviates hippocampal insulin resistance and differentially regulates radical species in cerebral cortex of mice. (A–F) Western blot analysis of lysates of cerebral cortex and hippocampus from male mice with or without probucol administration for 12 weeks. The levels of proteins were quantified in the left ( n = 4 mice per group). Cortex iNOS, ordinary one-way ANOVA ( p = 0.0296), followed by Tukey’s multiple comparisons test. Cortex NOX2, ordinary one-way ANOVA ( p = 0.0099), followed by Tukey’s multiple comparisons test. Hippocampal iNOS, ordinary one-way ANOVA ( p = 0.0025), followed by Tukey’s multiple comparisons test. Hippocampal NOX2, ordinary one-way ANOVA ( p = 0.2109). (G–L) Western blot analysis of AKT phosphorylation and PSD95 in the brain extracts of male mice with or without probucol administration for 5 weeks ( n = 4 mice per group). Cortex p-AKT, ordinary one-way ANOVA ( p = 0.9565). Cortex PSD95, ordinary one-way ANOVA ( p = 0.0547). Hippocampus p-AKT, ordinary one-way ANOVA ( p = 0.0179), followed by Tukey’s multiple comparisons test. Hippocampus PSD95, ordinary one-way ANOVA ( p = 0.0435), followed by Tukey’s multiple comparisons test. p < 0.05 was indicative of statistical significance.

    Journal: Frontiers in Neuroscience

    Article Title: Probucol mitigates high-fat diet-induced cognitive and social impairments by regulating brain redox and insulin resistance

    doi: 10.3389/fnins.2024.1368552

    Figure Lengend Snippet: Probucol alleviates hippocampal insulin resistance and differentially regulates radical species in cerebral cortex of mice. (A–F) Western blot analysis of lysates of cerebral cortex and hippocampus from male mice with or without probucol administration for 12 weeks. The levels of proteins were quantified in the left ( n = 4 mice per group). Cortex iNOS, ordinary one-way ANOVA ( p = 0.0296), followed by Tukey’s multiple comparisons test. Cortex NOX2, ordinary one-way ANOVA ( p = 0.0099), followed by Tukey’s multiple comparisons test. Hippocampal iNOS, ordinary one-way ANOVA ( p = 0.0025), followed by Tukey’s multiple comparisons test. Hippocampal NOX2, ordinary one-way ANOVA ( p = 0.2109). (G–L) Western blot analysis of AKT phosphorylation and PSD95 in the brain extracts of male mice with or without probucol administration for 5 weeks ( n = 4 mice per group). Cortex p-AKT, ordinary one-way ANOVA ( p = 0.9565). Cortex PSD95, ordinary one-way ANOVA ( p = 0.0547). Hippocampus p-AKT, ordinary one-way ANOVA ( p = 0.0179), followed by Tukey’s multiple comparisons test. Hippocampus PSD95, ordinary one-way ANOVA ( p = 0.0435), followed by Tukey’s multiple comparisons test. p < 0.05 was indicative of statistical significance.

    Article Snippet: In this study, the following primary antibodies were utilized for immunoblotting: iNOS polyclonal Antibody (1:1000, 18,985-1-AP, Proteintech, Wuhan, China), nicotinamide adenine dinucleotide phosphate oxidase 2 (NOX2) polyclonal antibody (1:5000, 19,013-1-AP, Proteintech), phospho-AKT (Ser473) polyclonal antibody (1:1000, 28,731-1-AP, Proteintech), AKT polyclonal antibody (1:2000, 10,176-2-AP, Proteintech), PSD95-specific, DLG4 polyclonal antibody (1:2000, 20,665-1-AP, Proteintech), and GAPDH antibody (1:50000, 60,004-1-Ig, Proteintech).

    Techniques: Western Blot

    Analysis of catalytic subunit NOX2 of NADPH oxidase in U-937 cells (A) and THP-1 cells (B) . Control (a) and proteins isolated from EVs co-cultured with U-937 cells (c). In b and d, U-937 and THP-1 cells were differentiated using 250 nM PMA and 150 nM PMA, respectively, in the absence and presence of EVs. In lanes e and f, differentiation was induced using 5 μM in the absence and presence of EVs, respectively, while in lanes f and h, 10 μM ascorbic acid was used. The densitometric quantification of NOX2 expression is presented in the lower panel within the figure.

    Journal: Frontiers in Cell and Developmental Biology

    Article Title: NOX2 and NOX4 expression in monocytes and macrophages-extracellular vesicles in signalling and therapeutics

    doi: 10.3389/fcell.2024.1342227

    Figure Lengend Snippet: Analysis of catalytic subunit NOX2 of NADPH oxidase in U-937 cells (A) and THP-1 cells (B) . Control (a) and proteins isolated from EVs co-cultured with U-937 cells (c). In b and d, U-937 and THP-1 cells were differentiated using 250 nM PMA and 150 nM PMA, respectively, in the absence and presence of EVs. In lanes e and f, differentiation was induced using 5 μM in the absence and presence of EVs, respectively, while in lanes f and h, 10 μM ascorbic acid was used. The densitometric quantification of NOX2 expression is presented in the lower panel within the figure.

    Article Snippet: CD63 monoclonal antibodies, NOX2 polyclonal antibody, and secondary antibody (HRP-conjugated goat anti-mouse) were obtained from Proteintech (GmbH Germany).

    Techniques: Isolation, Cell Culture, Expressing