normal igg rabbit ab  (Cell Signaling Technology Inc)

 
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    Name:
    Normal Rabbit IgG
    Description:
    Normal Rabbit IgG is an unconjugated rabbit polyclonal antibody that is routinely used as a non specific IgG control in chromatin immunoprecipitation using our SimpleChIP Enzymatic Chromatin IP Kits 9002 and 9003
    Catalog Number:
    2729
    Price:
    None
    Applications:
    Immunoprecipitation, Chromatin Immunoprecipitation
    Category:
    Primary Antibodies
    Source:
    The purified antibody is not directed against any known antigen. It was isolated from naive rabbit sera and prepared by Protein A chromatography.
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    Structured Review

    Cell Signaling Technology Inc normal igg rabbit ab
    Normal Rabbit IgG is an unconjugated rabbit polyclonal antibody that is routinely used as a non specific IgG control in chromatin immunoprecipitation using our SimpleChIP Enzymatic Chromatin IP Kits 9002 and 9003
    https://www.bioz.com/result/normal igg rabbit ab/product/Cell Signaling Technology Inc
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    normal igg rabbit ab - by Bioz Stars, 2020-08
    99/100 stars

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    Related Articles

    Sonication:

    Article Title: Atrophin controls developmental signaling pathways via interactions with Trithorax-like
    Article Snippet: .. ChIP-re-ChIP S2 cells were double crosslinked, sonicated, and ChIP’ed as above, using 5 μL anti-Atro sera (SG2524), and 10 μL normal rabbit IgG (Cell Signaling Technology). ..

    Incubation:

    Article Title: Squelching of ETS2 Transactivation by POU5F1 Silences the Human Chorionic Gonadotropin CGA Subunit Gene in Human Choriocarcinoma and Embryonic Stem Cells
    Article Snippet: .. Cell lysate containing 136 μg protein was incubated overnight with either 2 μg of anti-ETS2 antibody (sc-351; Santa Cruz Biotechnology) or normal rabbit IgG (Cell Signaling Technologies). .. Immune complexes were captured by the addition of a 50% slurry (40 μl) of protein G agarose beads (preadsorbed with BSA and sonicated salmon sperm DNA; Cell Signaling Technologies) and incubated for 24 h at 4 C. The beads were then washed three times in the buffer of 50 m m Tris-HCl (pH 8), 50 m m NaCl, 5 m m MgCl2 , 10% glycerol, 1% Nonidet P-40, 1 m m phenylmethylsulfonyl fluoride, protease inhibitor cocktail (Cell Signaling Technologies; 1 ml each).

    Article Title: MiR-509-3 augments the synthetic lethality of PARPi by regulating HR repair in PDX model of HGSOC
    Article Snippet: .. For co-immunoprecipitation with cell extracts, rabbit anti-human HMGA2 polyclonal antibody (Proteintech, 20,795-1-AP), normal rabbit IgG (CST, Cell Signaling Technology) was added and incubated for 12 h at 4 °C. .. A mixture of equal amounts of protein G Agarose (Fast Flow, Beyotime Biotechnology) was added and incubated for another 2 h at 4 °C.

    Binding Assay:

    Article Title: TDRD5 binds piRNA precursors and selectively enhances pachytene piRNA processing in mice
    Article Snippet: .. For each immunoprecipitation, TDRD5 antibody, MILI antibody (PM044, MBL), or Rabbit IgG (2729, Cell Signaling Technology) were bound on protein A Dynabeads (Life Technologies) in antibody binding buffer for 3 h at 4 °C. .. The beads were washed in antibody binding buffer, followed by 1X PMPG buffer, and incubated with lysates for 3 h at 4 °C.

    Chromatin Immunoprecipitation:

    Article Title: Gas6/Axl Axis Contributes to Chemoresistance and Metastasis in Breast Cancer through Akt/GSK-3β/β-catenin Signaling
    Article Snippet: .. The antibody or control IgG used for ChIP were as follows: rabbit anti-β-catenin (Cell Signaling Technology 8480), normal rabbit IgG (Cell Signaling Technology 2729). .. For the β-catenin/TCF binding site at position -161 of the human ZEB1 promoter, the primers amplifying the region between -325 and -101 were as follows: forward 5'-TTTACCTTTCCAACTCCGACAGC-3' and reverse 5-GGCTTTACGACATCACCTTCCTTAC-3.

    Article Title: Retinoic acid accelerates downregulation of the Xist repressor, Oct4, and increases the likelihood of Xist activation when Tsix is deficient
    Article Snippet: .. ChIP antibodies included goat polyclonal anti-Oct4 (Santa Cruz #sc8628) and normal rabbit IgG (Cell Signaling #2729). .. Quantitative PCR was performed using an iCycler iQ real-time PCR detection system (Bio-Rad) with primers specific to Xist intron 1 (site A: p63/p64, distanced 0.6 kb from site B: p65/p66).

    Article Title: Atrophin controls developmental signaling pathways via interactions with Trithorax-like
    Article Snippet: .. ChIP-re-ChIP S2 cells were double crosslinked, sonicated, and ChIP’ed as above, using 5 μL anti-Atro sera (SG2524), and 10 μL normal rabbit IgG (Cell Signaling Technology). ..

    Article Title: T-ALL leukemia stem cell 'stemness' is epigenetically controlled by the master regulator SPI1
    Article Snippet: .. The antibodies used for the ChIP assays were anti-SPI1 (sc-352, Santa Cruz) and normal rabbit IgG (2729, Cell Signaling Technology). .. The enriched regions were quantified by qPCR using the primers described in .

    Immunoprecipitation:

    Article Title: TDRD5 binds piRNA precursors and selectively enhances pachytene piRNA processing in mice
    Article Snippet: .. For each immunoprecipitation, TDRD5 antibody, MILI antibody (PM044, MBL), or Rabbit IgG (2729, Cell Signaling Technology) were bound on protein A Dynabeads (Life Technologies) in antibody binding buffer for 3 h at 4 °C. .. The beads were washed in antibody binding buffer, followed by 1X PMPG buffer, and incubated with lysates for 3 h at 4 °C.

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  • 99
    Cell Signaling Technology Inc normal rabbit igg
    Binding of <t>β-catenin</t> and TCF4 to human SLC6A14 promoter. Quantitative RT-PCR ( A ) and regular RT-PCR ( B ) for the region of SLC6A14 promoter with the binding motifs for β-catenin and TCF4 using the ChIP assay. Chromatin complexes from LS174T cells were immunoprecipitated using <t>IgG</t> (negative control) or antibodies specific for β-catenin and TCF4. Enrichment of SLC6A14 promoter was normalized with the starting amount of DNA (input).
    Normal Rabbit Igg, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 134 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/normal rabbit igg/product/Cell Signaling Technology Inc
    Average 99 stars, based on 134 article reviews
    Price from $9.99 to $1999.99
    normal rabbit igg - by Bioz Stars, 2020-08
    99/100 stars
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    Binding of β-catenin and TCF4 to human SLC6A14 promoter. Quantitative RT-PCR ( A ) and regular RT-PCR ( B ) for the region of SLC6A14 promoter with the binding motifs for β-catenin and TCF4 using the ChIP assay. Chromatin complexes from LS174T cells were immunoprecipitated using IgG (negative control) or antibodies specific for β-catenin and TCF4. Enrichment of SLC6A14 promoter was normalized with the starting amount of DNA (input).

    Journal: Biochemical Journal

    Article Title: SLC6A14, a Na+/Cl−-coupled amino acid transporter, functions as a tumor promoter in colon and is a target for Wnt signaling

    doi: 10.1042/BCJ20200099

    Figure Lengend Snippet: Binding of β-catenin and TCF4 to human SLC6A14 promoter. Quantitative RT-PCR ( A ) and regular RT-PCR ( B ) for the region of SLC6A14 promoter with the binding motifs for β-catenin and TCF4 using the ChIP assay. Chromatin complexes from LS174T cells were immunoprecipitated using IgG (negative control) or antibodies specific for β-catenin and TCF4. Enrichment of SLC6A14 promoter was normalized with the starting amount of DNA (input).

    Article Snippet: Antibodies Anti-mTOR (#2983S), anti-P-mTOR (#5536S), anti-S6K (#9202S), anti-P-S6K (#9204S), anti-LC3A/B (#4108S) anti-β-catenin (#8814S), anti-Cyclin D1 (#2922S), anti-TCF4 (#2569S), and anti-IgG (#2729S) antibodies were purchased from Cell Signaling Technology (Danvers, MA, U.S.A.).

    Techniques: Binding Assay, Quantitative RT-PCR, Reverse Transcription Polymerase Chain Reaction, Chromatin Immunoprecipitation, Immunoprecipitation, Negative Control

    SIRT1 regulates KISS1 expression through deacetylation of transcription factor CREB A. Gene expression profile array analysis in SW620 cells. Three-dimension (3D) profile of the expression values for mRNA of siRNA-SIRT1 cells versus siRNA-NC cells. B. Upregulation of KISS1 protein was detected when either knockdown of SIRT1 or overexpression of CREB by Western blot. C. ChIP assays for CREB and its binding motif. Antibodies anti-IgG and anti-CREB were used in the ChIP assays. QRT-PCR was performed to quantify the binding activity. D. KISS1 promoter constructs containing a potential CREB binding motif (−191 to −184 bp and −1961 to −1954 bp). The transcriptional activity of the KISS1 promoter was greatly increased after co-expression of CREB and KISS1 reporter in wild type. E. Endogenous SIRT1 immunoprecipitates with CREB. Proteins immunoprecipitated with rabbit IgG was used as control. F. Downregulation of SIRT1 increases the immunoprecipitated CREB levels detected using anti-acetyl lysine antibody. G. Western blot showing that SIRT1 overexpression suppresses forskolin-induced CREB-Ser 133 phosphorylation level. H. SIRT1 siRNA increases CRE transcriptional activity. Results are expressed as Luc/Renilla ratios. **P

    Journal: Oncotarget

    Article Title: Downregulation of miR-199b is associated with distant metastasis in colorectal cancer via activation of SIRT1 and inhibition of CREB/KISS1 signaling

    doi: 10.18632/oncotarget.9042

    Figure Lengend Snippet: SIRT1 regulates KISS1 expression through deacetylation of transcription factor CREB A. Gene expression profile array analysis in SW620 cells. Three-dimension (3D) profile of the expression values for mRNA of siRNA-SIRT1 cells versus siRNA-NC cells. B. Upregulation of KISS1 protein was detected when either knockdown of SIRT1 or overexpression of CREB by Western blot. C. ChIP assays for CREB and its binding motif. Antibodies anti-IgG and anti-CREB were used in the ChIP assays. QRT-PCR was performed to quantify the binding activity. D. KISS1 promoter constructs containing a potential CREB binding motif (−191 to −184 bp and −1961 to −1954 bp). The transcriptional activity of the KISS1 promoter was greatly increased after co-expression of CREB and KISS1 reporter in wild type. E. Endogenous SIRT1 immunoprecipitates with CREB. Proteins immunoprecipitated with rabbit IgG was used as control. F. Downregulation of SIRT1 increases the immunoprecipitated CREB levels detected using anti-acetyl lysine antibody. G. Western blot showing that SIRT1 overexpression suppresses forskolin-induced CREB-Ser 133 phosphorylation level. H. SIRT1 siRNA increases CRE transcriptional activity. Results are expressed as Luc/Renilla ratios. **P

    Article Snippet: A small portion (10%) of each sample was removed as an input control, and anti-CREB (9197#, Cell Signal Technology) and the rabbit normal IgG antibody (2729#, Cell Signal Technology) were added to the remaining samples.

    Techniques: Expressing, Over Expression, Western Blot, Chromatin Immunoprecipitation, Binding Assay, Quantitative RT-PCR, Activity Assay, Construct, Immunoprecipitation

    NSD3 dimethylates H3K36 at the Sox10 locus. (A) Primer locations in β-actin, Sox10, Snail2, Sox9 , and FoxD3 genes used for quantitative PCR after chromatin immunoprecipitation, named by their distance in kilobases from the transcription start site (arrow). (B) Average percentage input (mean ± SD) recovered in three independent chromatin immunoprecipitation experiments performed with 30 pooled NSD3 mmMO1- or MO1-electroporated neural tubes, assaying H3K36me2 occupancy at 12 genomic loci ( Chr1nc [chromosome 1 negative control], Chr2nc [chromosome 2 negative control], β-actin , four regions within Sox10 , three regions within Snail2 , Sox9 , and FoxD3 ). Input recovered is in the normal range for a methylated histone (Cell Signaling Technology, www.cellsignal.com/support/faq_chip.html#a11 ) and consistent with levels of H3K36me2 occupancy in other systems (e.g., Blackledge et al ., 2010 ; Asangani et al ., 2013 ; fold enrichment in Figure 5 ranges from 5 to 300). Normal rabbit IgG was an immunoprecipitation control (Ab control). Student's t test was used for statistical analysis; *significant; p

    Journal: Molecular Biology of the Cell

    Article Title: Neural crest specification and migration independently require NSD3-related lysine methyltransferase activity

    doi: 10.1091/mbc.E13-12-0744

    Figure Lengend Snippet: NSD3 dimethylates H3K36 at the Sox10 locus. (A) Primer locations in β-actin, Sox10, Snail2, Sox9 , and FoxD3 genes used for quantitative PCR after chromatin immunoprecipitation, named by their distance in kilobases from the transcription start site (arrow). (B) Average percentage input (mean ± SD) recovered in three independent chromatin immunoprecipitation experiments performed with 30 pooled NSD3 mmMO1- or MO1-electroporated neural tubes, assaying H3K36me2 occupancy at 12 genomic loci ( Chr1nc [chromosome 1 negative control], Chr2nc [chromosome 2 negative control], β-actin , four regions within Sox10 , three regions within Snail2 , Sox9 , and FoxD3 ). Input recovered is in the normal range for a methylated histone (Cell Signaling Technology, www.cellsignal.com/support/faq_chip.html#a11 ) and consistent with levels of H3K36me2 occupancy in other systems (e.g., Blackledge et al ., 2010 ; Asangani et al ., 2013 ; fold enrichment in Figure 5 ranges from 5 to 300). Normal rabbit IgG was an immunoprecipitation control (Ab control). Student's t test was used for statistical analysis; *significant; p

    Article Snippet: Equal volumes were combined with 5 μg of H3K36me2 antibody (Abcam, Cambridge, MA) or normal rabbit IgG antibody (Cell Signaling Technology) and incubated at 4°C overnight.

    Techniques: Real-time Polymerase Chain Reaction, Chromatin Immunoprecipitation, Negative Control, Methylation, Immunoprecipitation

    Chromatin marks at the IL12B locus. (A) A genome browser snapshot, including ChIP-seq tracks for α-H3K4me3, α-H3K4me1, α-H3K9me3, α-H3K27me3, α-C/EBPβ, α-H3K27ac, α-H3K36me3, IgG (unspecific polyclonal rabbit IgG pool) chromatin immunoprecipitations, and MIRA from a TAM sample freshly isolated from ovarian carcinoma ascites. Regions of interest are highlighted by rectangles. (B–D) ChIP-qPCR analyses of the indicated histone marks in an independent TAM sample at the indicated genomic locations amplified by specific primers are indicated. The error bars denote SDs from technical PCR replicates. (E–G) ChIP-qPCR analyses of the indicated histone marks in MDMs from three different pooled donor populations ( N = 3; N = 4 for IgG and α-H3K27me3 samples) differentiated in normal medium, in ascites, or in normal medium followed by ascites for 1 day. Cells were stimulated with or without LPS and IFNγ (+) or their respective solvents (−) 2.5 h prior to harvesting. Genomic regions were amplified by specific primers are indicated. Each dot denotes a biological replicate; for each replicate, MDMs from six donors were pooled after harvesting of the cells for each experiment. Median values are indicated by horizontal bars. Colors encode ascites samples from individual patients, and colors are consistent between panels within this figure, Figure 5 and Figure S9 in Supplementary Material. Statistical significances were calculated with paired t -tests. ** P

    Journal: Frontiers in Immunology

    Article Title: Chromatin Binding of c-REL and p65 Is Not Limiting for Macrophage IL12B Transcription During Immediate Suppression by Ovarian Carcinoma Ascites

    doi: 10.3389/fimmu.2018.01425

    Figure Lengend Snippet: Chromatin marks at the IL12B locus. (A) A genome browser snapshot, including ChIP-seq tracks for α-H3K4me3, α-H3K4me1, α-H3K9me3, α-H3K27me3, α-C/EBPβ, α-H3K27ac, α-H3K36me3, IgG (unspecific polyclonal rabbit IgG pool) chromatin immunoprecipitations, and MIRA from a TAM sample freshly isolated from ovarian carcinoma ascites. Regions of interest are highlighted by rectangles. (B–D) ChIP-qPCR analyses of the indicated histone marks in an independent TAM sample at the indicated genomic locations amplified by specific primers are indicated. The error bars denote SDs from technical PCR replicates. (E–G) ChIP-qPCR analyses of the indicated histone marks in MDMs from three different pooled donor populations ( N = 3; N = 4 for IgG and α-H3K27me3 samples) differentiated in normal medium, in ascites, or in normal medium followed by ascites for 1 day. Cells were stimulated with or without LPS and IFNγ (+) or their respective solvents (−) 2.5 h prior to harvesting. Genomic regions were amplified by specific primers are indicated. Each dot denotes a biological replicate; for each replicate, MDMs from six donors were pooled after harvesting of the cells for each experiment. Median values are indicated by horizontal bars. Colors encode ascites samples from individual patients, and colors are consistent between panels within this figure, Figure 5 and Figure S9 in Supplementary Material. Statistical significances were calculated with paired t -tests. ** P

    Article Snippet: ChIP was performed and evaluated as described using 4 µg per sample of the following antibodies: IgG pool, (Sigma-Aldrich Cat #I5006 RRID:AB_1163659) or normal rabbit IgG (Cell Signaling Technology Cat #2729S RRID:AB_1031062); α-c -REL (Santa Cruz Biotechnology Cat #sc-70 RRID:AB_2178727 and Santa Cruz Biotechnology Cat #sc-71 RRID:AB_2253705, 1:1 mixture); α-p65/RELA (Diagenode Cat #C15310256 RRID:AB_2721009); α-p65/RELA (Cell Signaling Technology Cat #8242 also 8242P, 8242S RRID:AB_10859369); α-H3K27me3 (Diagenode Cat #pAb-069-050 also ENCAB000ARJ RRID:AB_2616049); α-H3K4me3 (Diagenode Cat #pAb-003-050 also ENCAB000BKU, C15410003-50, C15410003-10 RRID:AB_2616052); α-H3K27ac (Diagenode, Diagenode, Cat #C15410174, RRID:AB_2716835); α-H3K4me1 (Diagenode Cat #pAb-037-050, RRID:AB_2561054); α-H3K9me3 (Diagenode Cat #pAb-056-050, RRID:AB_2616051); α-H3K36me3 (Abcam Cat #ab9050, RRID:AB_306966); α-C/EBPβ (Santa Cruz Biotechnology Cat #sc-150, RRID:AB_2260363).

    Techniques: Chromatin Immunoprecipitation, Isolation, Real-time Polymerase Chain Reaction, Amplification, Polymerase Chain Reaction