normal human ws1 dermal fibroblasts  (ATCC)


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    ATCC normal human ws1 dermal fibroblasts
    αSMA gene expression and protein levels are reduced in TGFβ1-activated <t>fibroblasts</t> following ketotifen treatment. ( A , B ) HDFa and ( C , D ) <t>WS1</t> fibroblasts were treated for 48 h with DMEM supplemented with 10% FBS as mock, 10 μM or 25 μM ketotifen, 10 ng/mL TGFβ1, or 10 ng/mL TGFβ1 with ketotifen added during the final 24 h. Gene expression of αSMA ( ACTA2 ) was measured using RT-qPCR and normalized to housekeeping gene HPRT . HDFa cells were treated for 48 h under the same conditions and probed for αSMA protein by western blot. ( E ) A representative image of the blots is shown (left) and semi-quantitative assessments plotted (right) using GAPDH as loading control. Full-length blots are available in Supplementary Fig. . ( F ) COL1A1 was measured in HDFa cells treated with conditions using 25 μM ketotifen. Protein levels of pro-collagen 1α1 ( G ) and fibronectin ( H ) were determined by ELISA staining kits. Data shown as mean ± SEM. n = 3–6 per treatment condition. * p < 0.05; **p < 0.01; *** p < 0.001; **** p < 0.0001. αSMA alpha-smooth muscle actin, GAPDH glyceraldehyde 3-phosphate dehydrogenase, HDFa human dermal fibroblasts (adult), SEM standard error of the mean, TGFβ1 transforming growth factor-beta.
    Normal Human Ws1 Dermal Fibroblasts, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/normal human ws1 dermal fibroblasts/product/ATCC
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    normal human ws1 dermal fibroblasts - by Bioz Stars, 2024-05
    86/100 stars

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    1) Product Images from "Ketotifen directly modifies the fibrotic response of human skin fibroblasts"

    Article Title: Ketotifen directly modifies the fibrotic response of human skin fibroblasts

    Journal: Scientific Reports

    doi: 10.1038/s41598-024-57776-7

    αSMA gene expression and protein levels are reduced in TGFβ1-activated fibroblasts following ketotifen treatment. ( A , B ) HDFa and ( C , D ) WS1 fibroblasts were treated for 48 h with DMEM supplemented with 10% FBS as mock, 10 μM or 25 μM ketotifen, 10 ng/mL TGFβ1, or 10 ng/mL TGFβ1 with ketotifen added during the final 24 h. Gene expression of αSMA ( ACTA2 ) was measured using RT-qPCR and normalized to housekeeping gene HPRT . HDFa cells were treated for 48 h under the same conditions and probed for αSMA protein by western blot. ( E ) A representative image of the blots is shown (left) and semi-quantitative assessments plotted (right) using GAPDH as loading control. Full-length blots are available in Supplementary Fig. . ( F ) COL1A1 was measured in HDFa cells treated with conditions using 25 μM ketotifen. Protein levels of pro-collagen 1α1 ( G ) and fibronectin ( H ) were determined by ELISA staining kits. Data shown as mean ± SEM. n = 3–6 per treatment condition. * p < 0.05; **p < 0.01; *** p < 0.001; **** p < 0.0001. αSMA alpha-smooth muscle actin, GAPDH glyceraldehyde 3-phosphate dehydrogenase, HDFa human dermal fibroblasts (adult), SEM standard error of the mean, TGFβ1 transforming growth factor-beta.
    Figure Legend Snippet: αSMA gene expression and protein levels are reduced in TGFβ1-activated fibroblasts following ketotifen treatment. ( A , B ) HDFa and ( C , D ) WS1 fibroblasts were treated for 48 h with DMEM supplemented with 10% FBS as mock, 10 μM or 25 μM ketotifen, 10 ng/mL TGFβ1, or 10 ng/mL TGFβ1 with ketotifen added during the final 24 h. Gene expression of αSMA ( ACTA2 ) was measured using RT-qPCR and normalized to housekeeping gene HPRT . HDFa cells were treated for 48 h under the same conditions and probed for αSMA protein by western blot. ( E ) A representative image of the blots is shown (left) and semi-quantitative assessments plotted (right) using GAPDH as loading control. Full-length blots are available in Supplementary Fig. . ( F ) COL1A1 was measured in HDFa cells treated with conditions using 25 μM ketotifen. Protein levels of pro-collagen 1α1 ( G ) and fibronectin ( H ) were determined by ELISA staining kits. Data shown as mean ± SEM. n = 3–6 per treatment condition. * p < 0.05; **p < 0.01; *** p < 0.001; **** p < 0.0001. αSMA alpha-smooth muscle actin, GAPDH glyceraldehyde 3-phosphate dehydrogenase, HDFa human dermal fibroblasts (adult), SEM standard error of the mean, TGFβ1 transforming growth factor-beta.

    Techniques Used: Expressing, Quantitative RT-PCR, Western Blot, Enzyme-linked Immunosorbent Assay, Staining

    Cytoskeletal-associated genes CNN1 and TAGLN are reduced with ketotifen treatment. HDFa and WS1 fibroblasts cells were treated for 48 h with DMEM supplemented with 10% FBS as mock, 10 μM or 25 μM ketotifen, 10 ng/mL TGFβ1, or 10 ng/mL TGFβ1 with ketotifen added during the final 24 h. CNN1 and TAGLN expression were assessed under 10 μM ketotifen (HDFa: A , B ; WS1: E , F ) and 25 μM ketotifen (HDFa: C , D ; WS1: G , H ) conditions. Data shown as mean ± SEM. n = 3–6 per treatment condition. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001. CNN1 calponin 1, HDFa human dermal fibroblasts (adult), SEM standard error of the mean, TAGLN transgelin, TGFβ1 transforming growth factor-beta 1.
    Figure Legend Snippet: Cytoskeletal-associated genes CNN1 and TAGLN are reduced with ketotifen treatment. HDFa and WS1 fibroblasts cells were treated for 48 h with DMEM supplemented with 10% FBS as mock, 10 μM or 25 μM ketotifen, 10 ng/mL TGFβ1, or 10 ng/mL TGFβ1 with ketotifen added during the final 24 h. CNN1 and TAGLN expression were assessed under 10 μM ketotifen (HDFa: A , B ; WS1: E , F ) and 25 μM ketotifen (HDFa: C , D ; WS1: G , H ) conditions. Data shown as mean ± SEM. n = 3–6 per treatment condition. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001. CNN1 calponin 1, HDFa human dermal fibroblasts (adult), SEM standard error of the mean, TAGLN transgelin, TGFβ1 transforming growth factor-beta 1.

    Techniques Used: Expressing

    ws1 normal human dermal fibroblasts  (ATCC)


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    Structured Review

    ATCC ws1 normal human dermal fibroblasts
    Ws1 Normal Human Dermal Fibroblasts, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ws1 normal human dermal fibroblasts/product/ATCC
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ws1 normal human dermal fibroblasts - by Bioz Stars, 2024-05
    86/100 stars

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    ATCC normal human ws1 dermal fibroblasts
    αSMA gene expression and protein levels are reduced in TGFβ1-activated <t>fibroblasts</t> following ketotifen treatment. ( A , B ) HDFa and ( C , D ) <t>WS1</t> fibroblasts were treated for 48 h with DMEM supplemented with 10% FBS as mock, 10 μM or 25 μM ketotifen, 10 ng/mL TGFβ1, or 10 ng/mL TGFβ1 with ketotifen added during the final 24 h. Gene expression of αSMA ( ACTA2 ) was measured using RT-qPCR and normalized to housekeeping gene HPRT . HDFa cells were treated for 48 h under the same conditions and probed for αSMA protein by western blot. ( E ) A representative image of the blots is shown (left) and semi-quantitative assessments plotted (right) using GAPDH as loading control. Full-length blots are available in Supplementary Fig. . ( F ) COL1A1 was measured in HDFa cells treated with conditions using 25 μM ketotifen. Protein levels of pro-collagen 1α1 ( G ) and fibronectin ( H ) were determined by ELISA staining kits. Data shown as mean ± SEM. n = 3–6 per treatment condition. * p < 0.05; **p < 0.01; *** p < 0.001; **** p < 0.0001. αSMA alpha-smooth muscle actin, GAPDH glyceraldehyde 3-phosphate dehydrogenase, HDFa human dermal fibroblasts (adult), SEM standard error of the mean, TGFβ1 transforming growth factor-beta.
    Normal Human Ws1 Dermal Fibroblasts, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/normal human ws1 dermal fibroblasts/product/ATCC
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    normal human ws1 dermal fibroblasts - by Bioz Stars, 2024-05
    86/100 stars
      Buy from Supplier

    86
    ATCC ws1 normal human dermal fibroblasts
    αSMA gene expression and protein levels are reduced in TGFβ1-activated <t>fibroblasts</t> following ketotifen treatment. ( A , B ) HDFa and ( C , D ) <t>WS1</t> fibroblasts were treated for 48 h with DMEM supplemented with 10% FBS as mock, 10 μM or 25 μM ketotifen, 10 ng/mL TGFβ1, or 10 ng/mL TGFβ1 with ketotifen added during the final 24 h. Gene expression of αSMA ( ACTA2 ) was measured using RT-qPCR and normalized to housekeeping gene HPRT . HDFa cells were treated for 48 h under the same conditions and probed for αSMA protein by western blot. ( E ) A representative image of the blots is shown (left) and semi-quantitative assessments plotted (right) using GAPDH as loading control. Full-length blots are available in Supplementary Fig. . ( F ) COL1A1 was measured in HDFa cells treated with conditions using 25 μM ketotifen. Protein levels of pro-collagen 1α1 ( G ) and fibronectin ( H ) were determined by ELISA staining kits. Data shown as mean ± SEM. n = 3–6 per treatment condition. * p < 0.05; **p < 0.01; *** p < 0.001; **** p < 0.0001. αSMA alpha-smooth muscle actin, GAPDH glyceraldehyde 3-phosphate dehydrogenase, HDFa human dermal fibroblasts (adult), SEM standard error of the mean, TGFβ1 transforming growth factor-beta.
    Ws1 Normal Human Dermal Fibroblasts, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ws1 normal human dermal fibroblasts/product/ATCC
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ws1 normal human dermal fibroblasts - by Bioz Stars, 2024-05
    86/100 stars
      Buy from Supplier

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    αSMA gene expression and protein levels are reduced in TGFβ1-activated fibroblasts following ketotifen treatment. ( A , B ) HDFa and ( C , D ) WS1 fibroblasts were treated for 48 h with DMEM supplemented with 10% FBS as mock, 10 μM or 25 μM ketotifen, 10 ng/mL TGFβ1, or 10 ng/mL TGFβ1 with ketotifen added during the final 24 h. Gene expression of αSMA ( ACTA2 ) was measured using RT-qPCR and normalized to housekeeping gene HPRT . HDFa cells were treated for 48 h under the same conditions and probed for αSMA protein by western blot. ( E ) A representative image of the blots is shown (left) and semi-quantitative assessments plotted (right) using GAPDH as loading control. Full-length blots are available in Supplementary Fig. . ( F ) COL1A1 was measured in HDFa cells treated with conditions using 25 μM ketotifen. Protein levels of pro-collagen 1α1 ( G ) and fibronectin ( H ) were determined by ELISA staining kits. Data shown as mean ± SEM. n = 3–6 per treatment condition. * p < 0.05; **p < 0.01; *** p < 0.001; **** p < 0.0001. αSMA alpha-smooth muscle actin, GAPDH glyceraldehyde 3-phosphate dehydrogenase, HDFa human dermal fibroblasts (adult), SEM standard error of the mean, TGFβ1 transforming growth factor-beta.

    Journal: Scientific Reports

    Article Title: Ketotifen directly modifies the fibrotic response of human skin fibroblasts

    doi: 10.1038/s41598-024-57776-7

    Figure Lengend Snippet: αSMA gene expression and protein levels are reduced in TGFβ1-activated fibroblasts following ketotifen treatment. ( A , B ) HDFa and ( C , D ) WS1 fibroblasts were treated for 48 h with DMEM supplemented with 10% FBS as mock, 10 μM or 25 μM ketotifen, 10 ng/mL TGFβ1, or 10 ng/mL TGFβ1 with ketotifen added during the final 24 h. Gene expression of αSMA ( ACTA2 ) was measured using RT-qPCR and normalized to housekeeping gene HPRT . HDFa cells were treated for 48 h under the same conditions and probed for αSMA protein by western blot. ( E ) A representative image of the blots is shown (left) and semi-quantitative assessments plotted (right) using GAPDH as loading control. Full-length blots are available in Supplementary Fig. . ( F ) COL1A1 was measured in HDFa cells treated with conditions using 25 μM ketotifen. Protein levels of pro-collagen 1α1 ( G ) and fibronectin ( H ) were determined by ELISA staining kits. Data shown as mean ± SEM. n = 3–6 per treatment condition. * p < 0.05; **p < 0.01; *** p < 0.001; **** p < 0.0001. αSMA alpha-smooth muscle actin, GAPDH glyceraldehyde 3-phosphate dehydrogenase, HDFa human dermal fibroblasts (adult), SEM standard error of the mean, TGFβ1 transforming growth factor-beta.

    Article Snippet: Normal primary human dermal fibroblasts (HDFa, PCS-201-012, ATCC, Manassas, VA; isolated from skin of a 27-year-old Asian male) and normal human WS1 dermal fibroblasts (CRL-1502, ATCC; isolated from skin of a Black female at 12-weeks of gestation) were used for in vitro experiments.

    Techniques: Expressing, Quantitative RT-PCR, Western Blot, Enzyme-linked Immunosorbent Assay, Staining

    Cytoskeletal-associated genes CNN1 and TAGLN are reduced with ketotifen treatment. HDFa and WS1 fibroblasts cells were treated for 48 h with DMEM supplemented with 10% FBS as mock, 10 μM or 25 μM ketotifen, 10 ng/mL TGFβ1, or 10 ng/mL TGFβ1 with ketotifen added during the final 24 h. CNN1 and TAGLN expression were assessed under 10 μM ketotifen (HDFa: A , B ; WS1: E , F ) and 25 μM ketotifen (HDFa: C , D ; WS1: G , H ) conditions. Data shown as mean ± SEM. n = 3–6 per treatment condition. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001. CNN1 calponin 1, HDFa human dermal fibroblasts (adult), SEM standard error of the mean, TAGLN transgelin, TGFβ1 transforming growth factor-beta 1.

    Journal: Scientific Reports

    Article Title: Ketotifen directly modifies the fibrotic response of human skin fibroblasts

    doi: 10.1038/s41598-024-57776-7

    Figure Lengend Snippet: Cytoskeletal-associated genes CNN1 and TAGLN are reduced with ketotifen treatment. HDFa and WS1 fibroblasts cells were treated for 48 h with DMEM supplemented with 10% FBS as mock, 10 μM or 25 μM ketotifen, 10 ng/mL TGFβ1, or 10 ng/mL TGFβ1 with ketotifen added during the final 24 h. CNN1 and TAGLN expression were assessed under 10 μM ketotifen (HDFa: A , B ; WS1: E , F ) and 25 μM ketotifen (HDFa: C , D ; WS1: G , H ) conditions. Data shown as mean ± SEM. n = 3–6 per treatment condition. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001. CNN1 calponin 1, HDFa human dermal fibroblasts (adult), SEM standard error of the mean, TAGLN transgelin, TGFβ1 transforming growth factor-beta 1.

    Article Snippet: Normal primary human dermal fibroblasts (HDFa, PCS-201-012, ATCC, Manassas, VA; isolated from skin of a 27-year-old Asian male) and normal human WS1 dermal fibroblasts (CRL-1502, ATCC; isolated from skin of a Black female at 12-weeks of gestation) were used for in vitro experiments.

    Techniques: Expressing