normal adult human primary dermal fibroblasts nhdf cells  (ATCC)


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    ATCC normal adult human primary dermal fibroblasts nhdf cells
    Cell viability and wound-healing assays. Notes: PPF leaves and stem picture. ( A ) PPF extract applied at 1 and 10 μg/mL showed no cytotoxicity in the EXCyto assay using <t>NHDF</t> cells. ( B ) The cell wound-healing assay was imaged 24 hours after scratching the cell monolayer and treating with PPF extract at the indicated doses. ( C ) Once cells reached confluence, a single wound was made in the center of the monolayer using a 10 μl pipette tip and the cells were treated with the indicated concentration of PPF extracts. After a 24-hour incubation, a photograph was taken at ×10 magnification under a microscope. ( D ) The photographic images from ( C ) in a dose-series were analyzed for gap area. The results are presented as the mean ± standard deviation of three independent experiments. ** P <0.01. Abbreviations: Conc, concentration; NHDF, normal human dermal fibroblast; PPF , Piper cambodianum P. Fourn.
    Normal Adult Human Primary Dermal Fibroblasts Nhdf Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Anti-aging effects of Piper cambodianum P. Fourn. extract on normal human dermal fibroblast cells and a wound-healing model in mice"

    Article Title: Anti-aging effects of Piper cambodianum P. Fourn. extract on normal human dermal fibroblast cells and a wound-healing model in mice

    Journal: Clinical Interventions in Aging

    doi: 10.2147/CIA.S107734

    Cell viability and wound-healing assays. Notes: PPF leaves and stem picture. ( A ) PPF extract applied at 1 and 10 μg/mL showed no cytotoxicity in the EXCyto assay using NHDF cells. ( B ) The cell wound-healing assay was imaged 24 hours after scratching the cell monolayer and treating with PPF extract at the indicated doses. ( C ) Once cells reached confluence, a single wound was made in the center of the monolayer using a 10 μl pipette tip and the cells were treated with the indicated concentration of PPF extracts. After a 24-hour incubation, a photograph was taken at ×10 magnification under a microscope. ( D ) The photographic images from ( C ) in a dose-series were analyzed for gap area. The results are presented as the mean ± standard deviation of three independent experiments. ** P <0.01. Abbreviations: Conc, concentration; NHDF, normal human dermal fibroblast; PPF , Piper cambodianum P. Fourn.
    Figure Legend Snippet: Cell viability and wound-healing assays. Notes: PPF leaves and stem picture. ( A ) PPF extract applied at 1 and 10 μg/mL showed no cytotoxicity in the EXCyto assay using NHDF cells. ( B ) The cell wound-healing assay was imaged 24 hours after scratching the cell monolayer and treating with PPF extract at the indicated doses. ( C ) Once cells reached confluence, a single wound was made in the center of the monolayer using a 10 μl pipette tip and the cells were treated with the indicated concentration of PPF extracts. After a 24-hour incubation, a photograph was taken at ×10 magnification under a microscope. ( D ) The photographic images from ( C ) in a dose-series were analyzed for gap area. The results are presented as the mean ± standard deviation of three independent experiments. ** P <0.01. Abbreviations: Conc, concentration; NHDF, normal human dermal fibroblast; PPF , Piper cambodianum P. Fourn.

    Techniques Used: Wound Healing Assay, Transferring, Concentration Assay, Incubation, Microscopy, Standard Deviation

    ECM gene expression level by q-PCR. Notes: RNAs from untreated or PPF -treated cells were analyzed by real-time q-PCR with ( A ) collagen-, ( B ) elastin-, ( C ) hyaluronan synthase (HAS)-2, and ( D ) matrix metalloproteinase (MMP)-1-specific primers. The results are presented as the mean ± SD of three independent experiments. * P <0.05, ** P <0.01. Abbreviations: Conc, concentration; ECM, extracellular matrix; HAS, hyaluronan synthase; MMP, matrix metalloproteinase; mRNA, messenger RNA; NHDF, normal human dermal fibroblast; PPF , Piper cambodianum P. Fourn.; q-PCR, quantitative polymerase chain reaction; RNA, ribonucleic acid; SD, standard deviation.
    Figure Legend Snippet: ECM gene expression level by q-PCR. Notes: RNAs from untreated or PPF -treated cells were analyzed by real-time q-PCR with ( A ) collagen-, ( B ) elastin-, ( C ) hyaluronan synthase (HAS)-2, and ( D ) matrix metalloproteinase (MMP)-1-specific primers. The results are presented as the mean ± SD of three independent experiments. * P <0.05, ** P <0.01. Abbreviations: Conc, concentration; ECM, extracellular matrix; HAS, hyaluronan synthase; MMP, matrix metalloproteinase; mRNA, messenger RNA; NHDF, normal human dermal fibroblast; PPF , Piper cambodianum P. Fourn.; q-PCR, quantitative polymerase chain reaction; RNA, ribonucleic acid; SD, standard deviation.

    Techniques Used: Expressing, Concentration Assay, Real-time Polymerase Chain Reaction, Standard Deviation

    Regulation of ECM proteins in PPF -treated cells. Notes: ( A ) NHDF cells were treated with PPF for 24 hours. Cell lysates were analyzed by immunoblotting using anti-collagen, anti-elastin, anti-MMP-3, and anti-ERK-2 antibodies. Results are representative of three independent experiments. ( B ) Actin, collagen, elastin, MMP-3, and ERK-2 in the immunoprecipitates were quantified by Western blotting. Bar heights are the respective mean ± SDs of three independent experiments. * P <0.05; ** P <0.01. Abbreviations: Conc, concentration; ECM, extracellular matrix; ERK, extracellular signal-regulated kinase; MMP, matrix metalloproteinase; NHDF, normal human dermal fibroblast; PPF , Piper cambodianum P. Fourn.; SDs, standard deviations.
    Figure Legend Snippet: Regulation of ECM proteins in PPF -treated cells. Notes: ( A ) NHDF cells were treated with PPF for 24 hours. Cell lysates were analyzed by immunoblotting using anti-collagen, anti-elastin, anti-MMP-3, and anti-ERK-2 antibodies. Results are representative of three independent experiments. ( B ) Actin, collagen, elastin, MMP-3, and ERK-2 in the immunoprecipitates were quantified by Western blotting. Bar heights are the respective mean ± SDs of three independent experiments. * P <0.05; ** P <0.01. Abbreviations: Conc, concentration; ECM, extracellular matrix; ERK, extracellular signal-regulated kinase; MMP, matrix metalloproteinase; NHDF, normal human dermal fibroblast; PPF , Piper cambodianum P. Fourn.; SDs, standard deviations.

    Techniques Used: Western Blot, Concentration Assay

    PPF extract inhibition of ROS production in UV-irradiated cells. Notes: ( A ) NHDF cells were exposed to UV irradiation at 40 J for 180 seconds and treated with PPF for 24 hours. Cell lysates were analyzed by immunoblotting using anti-TNFR6, anti-c-Jun, anti-c-Fos, anti-pp38, or anti-p-JNK antibodies. Results are representative of three independent experiments. ( B ) Total and tyrosine-phosphorylated p38, JUK, actin, and TNFR6 in the immunoprecipitates were quantified by Western analyses. Bar heights are mean ± SD of three independent experiments. * P <0.05; ** P <0.01. Abbreviations: Conc, concentration; EGFR, epidermal growth factor receptor; JNK, c-Jun amino terminal kinase; JNK, c-Jun N-terminal kinase; NHDF, normal human dermal fibroblast; PPF , Piper cambodianum P. Fourn.; ROS, reactive oxygen species; SD, standard deviation; TNFR, tumor necrosis factor receptor; UV, ultraviolet.
    Figure Legend Snippet: PPF extract inhibition of ROS production in UV-irradiated cells. Notes: ( A ) NHDF cells were exposed to UV irradiation at 40 J for 180 seconds and treated with PPF for 24 hours. Cell lysates were analyzed by immunoblotting using anti-TNFR6, anti-c-Jun, anti-c-Fos, anti-pp38, or anti-p-JNK antibodies. Results are representative of three independent experiments. ( B ) Total and tyrosine-phosphorylated p38, JUK, actin, and TNFR6 in the immunoprecipitates were quantified by Western analyses. Bar heights are mean ± SD of three independent experiments. * P <0.05; ** P <0.01. Abbreviations: Conc, concentration; EGFR, epidermal growth factor receptor; JNK, c-Jun amino terminal kinase; JNK, c-Jun N-terminal kinase; NHDF, normal human dermal fibroblast; PPF , Piper cambodianum P. Fourn.; ROS, reactive oxygen species; SD, standard deviation; TNFR, tumor necrosis factor receptor; UV, ultraviolet.

    Techniques Used: Inhibition, Irradiation, Western Blot, Concentration Assay, Standard Deviation

    PPF -mediated inhibition of ROS production in UV-irradiated cells. Notes: ( A ) Cells were treated with UVB irradiation (40 J) for the indicated time and then incubated with 2′,7′-dichlorodihydrofluorescein diacetate for 30 minutes. The generation of ROS was measured by FACS. ( B ) Cells were exposed to UVB (40 J) for 180 seconds and treated with the indicated concentration of PPF for 24 hours. ROS production were estimated with the staining of 2′,7′-dichlorodihydrofluorescein diacetate and analyzed by FACS. Abbreviations: FACS, fluorescence-activated cell sorting; FITC, fluorescein isothiocyanate; NHDF, normal human dermal fibroblast; PPF , Piper cambodianum P. Fourn.; ROS, reactive oxygen species; UV, ultraviolet; UVB, ultraviolet B.
    Figure Legend Snippet: PPF -mediated inhibition of ROS production in UV-irradiated cells. Notes: ( A ) Cells were treated with UVB irradiation (40 J) for the indicated time and then incubated with 2′,7′-dichlorodihydrofluorescein diacetate for 30 minutes. The generation of ROS was measured by FACS. ( B ) Cells were exposed to UVB (40 J) for 180 seconds and treated with the indicated concentration of PPF for 24 hours. ROS production were estimated with the staining of 2′,7′-dichlorodihydrofluorescein diacetate and analyzed by FACS. Abbreviations: FACS, fluorescence-activated cell sorting; FITC, fluorescein isothiocyanate; NHDF, normal human dermal fibroblast; PPF , Piper cambodianum P. Fourn.; ROS, reactive oxygen species; UV, ultraviolet; UVB, ultraviolet B.

    Techniques Used: Inhibition, Irradiation, Incubation, Concentration Assay, Staining, Fluorescence, FACS

    normal human dermal fibroblasts nhdfs  (ATCC)


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    ATCC normal human dermal fibroblasts nhdfs
    Normal Human Dermal Fibroblasts Nhdfs, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    normal human dermal fibroblast nhdf  (ATCC)


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    ATCC normal human dermal fibroblast nhdf
    A UMAP plot of 33,118 FBs coloured by cell subtype. Each dot denotes a single cell. B Heatmap showing differences in hallmark pathway activities scored per cell with GSVA among different <t>fibroblast</t> subtypes. ( C ) Dot plot of the expression levels of marker genes for FB subtypes defined in A . Dot size corresponds to the percent of expressing cells, and dot colour indicates the average expression levels. D UMAP plot of all fibroblast subtypes from the skin of the IMQ group and WT group, coloured by cell type. E Bulk RNA-seq and scRNA-seq analysis of the DEGs in the IMQ group and WT group (bulk RNA-seq data source GSM2299980 (WT), GSM2299981 (WT), GSM229998 (IMQ), GSM2299983 (IMQ)). F Violin plots showing the top DEGs between IMQ and WT in all fibroblast subtypes. G KEGG pathway map of IMQ vs. WT scRNA-seq in FBs. H Western blot image of Vim, Lcn2 and IL-17 expressions in HaCaT cells treated with IL-17 for indicated time. I – K Histogram of protein expression level in H . L – N Quantification of Vim, Lcn2 and IL-17 mRNA expressions in <t>NHDF</t> cells stimulated with IL-17 (20 ng/mL) for 6 h.
    Normal Human Dermal Fibroblast Nhdf, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Single-cell RNA-seq reveals keratinocyte and fibroblast heterogeneity and their crosstalk via epithelial-mesenchymal transition in psoriasis"

    Article Title: Single-cell RNA-seq reveals keratinocyte and fibroblast heterogeneity and their crosstalk via epithelial-mesenchymal transition in psoriasis

    Journal: Cell Death & Disease

    doi: 10.1038/s41419-024-06583-z

    A UMAP plot of 33,118 FBs coloured by cell subtype. Each dot denotes a single cell. B Heatmap showing differences in hallmark pathway activities scored per cell with GSVA among different fibroblast subtypes. ( C ) Dot plot of the expression levels of marker genes for FB subtypes defined in A . Dot size corresponds to the percent of expressing cells, and dot colour indicates the average expression levels. D UMAP plot of all fibroblast subtypes from the skin of the IMQ group and WT group, coloured by cell type. E Bulk RNA-seq and scRNA-seq analysis of the DEGs in the IMQ group and WT group (bulk RNA-seq data source GSM2299980 (WT), GSM2299981 (WT), GSM229998 (IMQ), GSM2299983 (IMQ)). F Violin plots showing the top DEGs between IMQ and WT in all fibroblast subtypes. G KEGG pathway map of IMQ vs. WT scRNA-seq in FBs. H Western blot image of Vim, Lcn2 and IL-17 expressions in HaCaT cells treated with IL-17 for indicated time. I – K Histogram of protein expression level in H . L – N Quantification of Vim, Lcn2 and IL-17 mRNA expressions in NHDF cells stimulated with IL-17 (20 ng/mL) for 6 h.
    Figure Legend Snippet: A UMAP plot of 33,118 FBs coloured by cell subtype. Each dot denotes a single cell. B Heatmap showing differences in hallmark pathway activities scored per cell with GSVA among different fibroblast subtypes. ( C ) Dot plot of the expression levels of marker genes for FB subtypes defined in A . Dot size corresponds to the percent of expressing cells, and dot colour indicates the average expression levels. D UMAP plot of all fibroblast subtypes from the skin of the IMQ group and WT group, coloured by cell type. E Bulk RNA-seq and scRNA-seq analysis of the DEGs in the IMQ group and WT group (bulk RNA-seq data source GSM2299980 (WT), GSM2299981 (WT), GSM229998 (IMQ), GSM2299983 (IMQ)). F Violin plots showing the top DEGs between IMQ and WT in all fibroblast subtypes. G KEGG pathway map of IMQ vs. WT scRNA-seq in FBs. H Western blot image of Vim, Lcn2 and IL-17 expressions in HaCaT cells treated with IL-17 for indicated time. I – K Histogram of protein expression level in H . L – N Quantification of Vim, Lcn2 and IL-17 mRNA expressions in NHDF cells stimulated with IL-17 (20 ng/mL) for 6 h.

    Techniques Used: Expressing, Marker, RNA Sequencing Assay, Western Blot

    normal human dermal fibroblasts nhdfs  (ATCC)


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    ATCC normal human dermal fibroblasts nhdfs
    Normal Human Dermal Fibroblasts Nhdfs, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    normal human dermal fibroblasts nhdfs  (ATCC)


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    ATCC normal human dermal fibroblasts nhdfs
    Antioxidant effects of essential oils on skin dermal <t>fibroblasts.</t> The protective effect of <t>NHDFs</t> on H 2 O 2 ‐induced oxidative stress was investigated using the MTT assay ( n = 3). Results are expressed as means ± SDs. Statistical analysis: t ‐test; * P < 0.05 significantly different versus control group not treated with H 2 O 2 . # P < 0.05 significantly different with H 2 O 2 treated group.
    Normal Human Dermal Fibroblasts Nhdfs, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Essential oils extracted from nine different plants exhibit differential effects on skin antioxidation and elasticity"

    Article Title: Essential oils extracted from nine different plants exhibit differential effects on skin antioxidation and elasticity

    Journal: FEBS Open Bio

    doi: 10.1002/2211-5463.13778

    Antioxidant effects of essential oils on skin dermal fibroblasts. The protective effect of NHDFs on H 2 O 2 ‐induced oxidative stress was investigated using the MTT assay ( n = 3). Results are expressed as means ± SDs. Statistical analysis: t ‐test; * P < 0.05 significantly different versus control group not treated with H 2 O 2 . # P < 0.05 significantly different with H 2 O 2 treated group.
    Figure Legend Snippet: Antioxidant effects of essential oils on skin dermal fibroblasts. The protective effect of NHDFs on H 2 O 2 ‐induced oxidative stress was investigated using the MTT assay ( n = 3). Results are expressed as means ± SDs. Statistical analysis: t ‐test; * P < 0.05 significantly different versus control group not treated with H 2 O 2 . # P < 0.05 significantly different with H 2 O 2 treated group.

    Techniques Used: MTT Assay

    Effects of the five essential oils on skin fibroblast proliferation. The proliferations of NHDFs treated with the five essential oils were estimated using the BrdU assay ( n = 3). Results are expressed as means ± SDs. Statistical analysis: t ‐test; * P < 0.05 significant difference compared to the control.
    Figure Legend Snippet: Effects of the five essential oils on skin fibroblast proliferation. The proliferations of NHDFs treated with the five essential oils were estimated using the BrdU assay ( n = 3). Results are expressed as means ± SDs. Statistical analysis: t ‐test; * P < 0.05 significant difference compared to the control.

    Techniques Used: BrdU Staining

    Translational levels of ECM proteins in NHDFs treated with the five essential oils. The protein expressions of ECM proteins were measured by western blot to evaluate protein production by fibroblasts treated with the five essential oils (A). The expression levels of (B) collagen 1, (C) collagen3, and (D) elastin are represented as graphs normalized versus GAPDH levels ( n = 3). Results are expressed as means ± SDs. Statistical analysis: t ‐test; * P < 0.05 significantly different versus controls.
    Figure Legend Snippet: Translational levels of ECM proteins in NHDFs treated with the five essential oils. The protein expressions of ECM proteins were measured by western blot to evaluate protein production by fibroblasts treated with the five essential oils (A). The expression levels of (B) collagen 1, (C) collagen3, and (D) elastin are represented as graphs normalized versus GAPDH levels ( n = 3). Results are expressed as means ± SDs. Statistical analysis: t ‐test; * P < 0.05 significantly different versus controls.

    Techniques Used: Western Blot, Expressing

    dmem atcc adherent normal human normal adult human primary dmem atcc dermal fibroblasts dermal fibroblasts nhdf  (ATCC)


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    ATCC dmem atcc adherent normal human normal adult human primary dmem atcc dermal fibroblasts dermal fibroblasts nhdf
    Dmem Atcc Adherent Normal Human Normal Adult Human Primary Dmem Atcc Dermal Fibroblasts Dermal Fibroblasts Nhdf, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    culture conditions normal human dermal fibroblasts nhdf  (ATCC)


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    ATCC culture conditions normal human dermal fibroblasts nhdf
    Culture Conditions Normal Human Dermal Fibroblasts Nhdf, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    normal human dermal fibroblasts nhdfs  (ATCC)


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    ATCC normal human dermal fibroblasts nhdfs
    Normal Human Dermal Fibroblasts Nhdfs, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    cxcl1 inhibition assay normal human dermal fibroblasts nhdf  (ATCC)


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    ATCC cxcl1 inhibition assay normal human dermal fibroblasts nhdf
    Cxcl1 Inhibition Assay Normal Human Dermal Fibroblasts Nhdf, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    normal adult human primary dermal fibroblasts nhdf cells  (ATCC)


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    ATCC normal adult human primary dermal fibroblasts nhdf cells
    Cell viability and wound-healing assays. Notes: PPF leaves and stem picture. ( A ) PPF extract applied at 1 and 10 μg/mL showed no cytotoxicity in the EXCyto assay using <t>NHDF</t> cells. ( B ) The cell wound-healing assay was imaged 24 hours after scratching the cell monolayer and treating with PPF extract at the indicated doses. ( C ) Once cells reached confluence, a single wound was made in the center of the monolayer using a 10 μl pipette tip and the cells were treated with the indicated concentration of PPF extracts. After a 24-hour incubation, a photograph was taken at ×10 magnification under a microscope. ( D ) The photographic images from ( C ) in a dose-series were analyzed for gap area. The results are presented as the mean ± standard deviation of three independent experiments. ** P <0.01. Abbreviations: Conc, concentration; NHDF, normal human dermal fibroblast; PPF , Piper cambodianum P. Fourn.
    Normal Adult Human Primary Dermal Fibroblasts Nhdf Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Anti-aging effects of Piper cambodianum P. Fourn. extract on normal human dermal fibroblast cells and a wound-healing model in mice"

    Article Title: Anti-aging effects of Piper cambodianum P. Fourn. extract on normal human dermal fibroblast cells and a wound-healing model in mice

    Journal: Clinical Interventions in Aging

    doi: 10.2147/CIA.S107734

    Cell viability and wound-healing assays. Notes: PPF leaves and stem picture. ( A ) PPF extract applied at 1 and 10 μg/mL showed no cytotoxicity in the EXCyto assay using NHDF cells. ( B ) The cell wound-healing assay was imaged 24 hours after scratching the cell monolayer and treating with PPF extract at the indicated doses. ( C ) Once cells reached confluence, a single wound was made in the center of the monolayer using a 10 μl pipette tip and the cells were treated with the indicated concentration of PPF extracts. After a 24-hour incubation, a photograph was taken at ×10 magnification under a microscope. ( D ) The photographic images from ( C ) in a dose-series were analyzed for gap area. The results are presented as the mean ± standard deviation of three independent experiments. ** P <0.01. Abbreviations: Conc, concentration; NHDF, normal human dermal fibroblast; PPF , Piper cambodianum P. Fourn.
    Figure Legend Snippet: Cell viability and wound-healing assays. Notes: PPF leaves and stem picture. ( A ) PPF extract applied at 1 and 10 μg/mL showed no cytotoxicity in the EXCyto assay using NHDF cells. ( B ) The cell wound-healing assay was imaged 24 hours after scratching the cell monolayer and treating with PPF extract at the indicated doses. ( C ) Once cells reached confluence, a single wound was made in the center of the monolayer using a 10 μl pipette tip and the cells were treated with the indicated concentration of PPF extracts. After a 24-hour incubation, a photograph was taken at ×10 magnification under a microscope. ( D ) The photographic images from ( C ) in a dose-series were analyzed for gap area. The results are presented as the mean ± standard deviation of three independent experiments. ** P <0.01. Abbreviations: Conc, concentration; NHDF, normal human dermal fibroblast; PPF , Piper cambodianum P. Fourn.

    Techniques Used: Wound Healing Assay, Transferring, Concentration Assay, Incubation, Microscopy, Standard Deviation

    ECM gene expression level by q-PCR. Notes: RNAs from untreated or PPF -treated cells were analyzed by real-time q-PCR with ( A ) collagen-, ( B ) elastin-, ( C ) hyaluronan synthase (HAS)-2, and ( D ) matrix metalloproteinase (MMP)-1-specific primers. The results are presented as the mean ± SD of three independent experiments. * P <0.05, ** P <0.01. Abbreviations: Conc, concentration; ECM, extracellular matrix; HAS, hyaluronan synthase; MMP, matrix metalloproteinase; mRNA, messenger RNA; NHDF, normal human dermal fibroblast; PPF , Piper cambodianum P. Fourn.; q-PCR, quantitative polymerase chain reaction; RNA, ribonucleic acid; SD, standard deviation.
    Figure Legend Snippet: ECM gene expression level by q-PCR. Notes: RNAs from untreated or PPF -treated cells were analyzed by real-time q-PCR with ( A ) collagen-, ( B ) elastin-, ( C ) hyaluronan synthase (HAS)-2, and ( D ) matrix metalloproteinase (MMP)-1-specific primers. The results are presented as the mean ± SD of three independent experiments. * P <0.05, ** P <0.01. Abbreviations: Conc, concentration; ECM, extracellular matrix; HAS, hyaluronan synthase; MMP, matrix metalloproteinase; mRNA, messenger RNA; NHDF, normal human dermal fibroblast; PPF , Piper cambodianum P. Fourn.; q-PCR, quantitative polymerase chain reaction; RNA, ribonucleic acid; SD, standard deviation.

    Techniques Used: Expressing, Concentration Assay, Real-time Polymerase Chain Reaction, Standard Deviation

    Regulation of ECM proteins in PPF -treated cells. Notes: ( A ) NHDF cells were treated with PPF for 24 hours. Cell lysates were analyzed by immunoblotting using anti-collagen, anti-elastin, anti-MMP-3, and anti-ERK-2 antibodies. Results are representative of three independent experiments. ( B ) Actin, collagen, elastin, MMP-3, and ERK-2 in the immunoprecipitates were quantified by Western blotting. Bar heights are the respective mean ± SDs of three independent experiments. * P <0.05; ** P <0.01. Abbreviations: Conc, concentration; ECM, extracellular matrix; ERK, extracellular signal-regulated kinase; MMP, matrix metalloproteinase; NHDF, normal human dermal fibroblast; PPF , Piper cambodianum P. Fourn.; SDs, standard deviations.
    Figure Legend Snippet: Regulation of ECM proteins in PPF -treated cells. Notes: ( A ) NHDF cells were treated with PPF for 24 hours. Cell lysates were analyzed by immunoblotting using anti-collagen, anti-elastin, anti-MMP-3, and anti-ERK-2 antibodies. Results are representative of three independent experiments. ( B ) Actin, collagen, elastin, MMP-3, and ERK-2 in the immunoprecipitates were quantified by Western blotting. Bar heights are the respective mean ± SDs of three independent experiments. * P <0.05; ** P <0.01. Abbreviations: Conc, concentration; ECM, extracellular matrix; ERK, extracellular signal-regulated kinase; MMP, matrix metalloproteinase; NHDF, normal human dermal fibroblast; PPF , Piper cambodianum P. Fourn.; SDs, standard deviations.

    Techniques Used: Western Blot, Concentration Assay

    PPF extract inhibition of ROS production in UV-irradiated cells. Notes: ( A ) NHDF cells were exposed to UV irradiation at 40 J for 180 seconds and treated with PPF for 24 hours. Cell lysates were analyzed by immunoblotting using anti-TNFR6, anti-c-Jun, anti-c-Fos, anti-pp38, or anti-p-JNK antibodies. Results are representative of three independent experiments. ( B ) Total and tyrosine-phosphorylated p38, JUK, actin, and TNFR6 in the immunoprecipitates were quantified by Western analyses. Bar heights are mean ± SD of three independent experiments. * P <0.05; ** P <0.01. Abbreviations: Conc, concentration; EGFR, epidermal growth factor receptor; JNK, c-Jun amino terminal kinase; JNK, c-Jun N-terminal kinase; NHDF, normal human dermal fibroblast; PPF , Piper cambodianum P. Fourn.; ROS, reactive oxygen species; SD, standard deviation; TNFR, tumor necrosis factor receptor; UV, ultraviolet.
    Figure Legend Snippet: PPF extract inhibition of ROS production in UV-irradiated cells. Notes: ( A ) NHDF cells were exposed to UV irradiation at 40 J for 180 seconds and treated with PPF for 24 hours. Cell lysates were analyzed by immunoblotting using anti-TNFR6, anti-c-Jun, anti-c-Fos, anti-pp38, or anti-p-JNK antibodies. Results are representative of three independent experiments. ( B ) Total and tyrosine-phosphorylated p38, JUK, actin, and TNFR6 in the immunoprecipitates were quantified by Western analyses. Bar heights are mean ± SD of three independent experiments. * P <0.05; ** P <0.01. Abbreviations: Conc, concentration; EGFR, epidermal growth factor receptor; JNK, c-Jun amino terminal kinase; JNK, c-Jun N-terminal kinase; NHDF, normal human dermal fibroblast; PPF , Piper cambodianum P. Fourn.; ROS, reactive oxygen species; SD, standard deviation; TNFR, tumor necrosis factor receptor; UV, ultraviolet.

    Techniques Used: Inhibition, Irradiation, Western Blot, Concentration Assay, Standard Deviation

    PPF -mediated inhibition of ROS production in UV-irradiated cells. Notes: ( A ) Cells were treated with UVB irradiation (40 J) for the indicated time and then incubated with 2′,7′-dichlorodihydrofluorescein diacetate for 30 minutes. The generation of ROS was measured by FACS. ( B ) Cells were exposed to UVB (40 J) for 180 seconds and treated with the indicated concentration of PPF for 24 hours. ROS production were estimated with the staining of 2′,7′-dichlorodihydrofluorescein diacetate and analyzed by FACS. Abbreviations: FACS, fluorescence-activated cell sorting; FITC, fluorescein isothiocyanate; NHDF, normal human dermal fibroblast; PPF , Piper cambodianum P. Fourn.; ROS, reactive oxygen species; UV, ultraviolet; UVB, ultraviolet B.
    Figure Legend Snippet: PPF -mediated inhibition of ROS production in UV-irradiated cells. Notes: ( A ) Cells were treated with UVB irradiation (40 J) for the indicated time and then incubated with 2′,7′-dichlorodihydrofluorescein diacetate for 30 minutes. The generation of ROS was measured by FACS. ( B ) Cells were exposed to UVB (40 J) for 180 seconds and treated with the indicated concentration of PPF for 24 hours. ROS production were estimated with the staining of 2′,7′-dichlorodihydrofluorescein diacetate and analyzed by FACS. Abbreviations: FACS, fluorescence-activated cell sorting; FITC, fluorescein isothiocyanate; NHDF, normal human dermal fibroblast; PPF , Piper cambodianum P. Fourn.; ROS, reactive oxygen species; UV, ultraviolet; UVB, ultraviolet B.

    Techniques Used: Inhibition, Irradiation, Incubation, Concentration Assay, Staining, Fluorescence, FACS

    normal human dermal fibroblast adult nhdf ad cell lines  (ATCC)


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    ATCC normal human dermal fibroblast adult nhdf ad cell lines
    Normal Human Dermal Fibroblast Adult Nhdf Ad Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC normal adult human primary dermal fibroblasts nhdf cells
    Cell viability and wound-healing assays. Notes: PPF leaves and stem picture. ( A ) PPF extract applied at 1 and 10 μg/mL showed no cytotoxicity in the EXCyto assay using <t>NHDF</t> cells. ( B ) The cell wound-healing assay was imaged 24 hours after scratching the cell monolayer and treating with PPF extract at the indicated doses. ( C ) Once cells reached confluence, a single wound was made in the center of the monolayer using a 10 μl pipette tip and the cells were treated with the indicated concentration of PPF extracts. After a 24-hour incubation, a photograph was taken at ×10 magnification under a microscope. ( D ) The photographic images from ( C ) in a dose-series were analyzed for gap area. The results are presented as the mean ± standard deviation of three independent experiments. ** P <0.01. Abbreviations: Conc, concentration; NHDF, normal human dermal fibroblast; PPF , Piper cambodianum P. Fourn.
    Normal Adult Human Primary Dermal Fibroblasts Nhdf Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC normal human dermal fibroblasts nhdfs
    Cell viability and wound-healing assays. Notes: PPF leaves and stem picture. ( A ) PPF extract applied at 1 and 10 μg/mL showed no cytotoxicity in the EXCyto assay using <t>NHDF</t> cells. ( B ) The cell wound-healing assay was imaged 24 hours after scratching the cell monolayer and treating with PPF extract at the indicated doses. ( C ) Once cells reached confluence, a single wound was made in the center of the monolayer using a 10 μl pipette tip and the cells were treated with the indicated concentration of PPF extracts. After a 24-hour incubation, a photograph was taken at ×10 magnification under a microscope. ( D ) The photographic images from ( C ) in a dose-series were analyzed for gap area. The results are presented as the mean ± standard deviation of three independent experiments. ** P <0.01. Abbreviations: Conc, concentration; NHDF, normal human dermal fibroblast; PPF , Piper cambodianum P. Fourn.
    Normal Human Dermal Fibroblasts Nhdfs, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC normal human dermal fibroblast nhdf
    A UMAP plot of 33,118 FBs coloured by cell subtype. Each dot denotes a single cell. B Heatmap showing differences in hallmark pathway activities scored per cell with GSVA among different <t>fibroblast</t> subtypes. ( C ) Dot plot of the expression levels of marker genes for FB subtypes defined in A . Dot size corresponds to the percent of expressing cells, and dot colour indicates the average expression levels. D UMAP plot of all fibroblast subtypes from the skin of the IMQ group and WT group, coloured by cell type. E Bulk RNA-seq and scRNA-seq analysis of the DEGs in the IMQ group and WT group (bulk RNA-seq data source GSM2299980 (WT), GSM2299981 (WT), GSM229998 (IMQ), GSM2299983 (IMQ)). F Violin plots showing the top DEGs between IMQ and WT in all fibroblast subtypes. G KEGG pathway map of IMQ vs. WT scRNA-seq in FBs. H Western blot image of Vim, Lcn2 and IL-17 expressions in HaCaT cells treated with IL-17 for indicated time. I – K Histogram of protein expression level in H . L – N Quantification of Vim, Lcn2 and IL-17 mRNA expressions in <t>NHDF</t> cells stimulated with IL-17 (20 ng/mL) for 6 h.
    Normal Human Dermal Fibroblast Nhdf, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC dmem atcc adherent normal human normal adult human primary dmem atcc dermal fibroblasts dermal fibroblasts nhdf
    A UMAP plot of 33,118 FBs coloured by cell subtype. Each dot denotes a single cell. B Heatmap showing differences in hallmark pathway activities scored per cell with GSVA among different <t>fibroblast</t> subtypes. ( C ) Dot plot of the expression levels of marker genes for FB subtypes defined in A . Dot size corresponds to the percent of expressing cells, and dot colour indicates the average expression levels. D UMAP plot of all fibroblast subtypes from the skin of the IMQ group and WT group, coloured by cell type. E Bulk RNA-seq and scRNA-seq analysis of the DEGs in the IMQ group and WT group (bulk RNA-seq data source GSM2299980 (WT), GSM2299981 (WT), GSM229998 (IMQ), GSM2299983 (IMQ)). F Violin plots showing the top DEGs between IMQ and WT in all fibroblast subtypes. G KEGG pathway map of IMQ vs. WT scRNA-seq in FBs. H Western blot image of Vim, Lcn2 and IL-17 expressions in HaCaT cells treated with IL-17 for indicated time. I – K Histogram of protein expression level in H . L – N Quantification of Vim, Lcn2 and IL-17 mRNA expressions in <t>NHDF</t> cells stimulated with IL-17 (20 ng/mL) for 6 h.
    Dmem Atcc Adherent Normal Human Normal Adult Human Primary Dmem Atcc Dermal Fibroblasts Dermal Fibroblasts Nhdf, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC culture conditions normal human dermal fibroblasts nhdf
    A UMAP plot of 33,118 FBs coloured by cell subtype. Each dot denotes a single cell. B Heatmap showing differences in hallmark pathway activities scored per cell with GSVA among different <t>fibroblast</t> subtypes. ( C ) Dot plot of the expression levels of marker genes for FB subtypes defined in A . Dot size corresponds to the percent of expressing cells, and dot colour indicates the average expression levels. D UMAP plot of all fibroblast subtypes from the skin of the IMQ group and WT group, coloured by cell type. E Bulk RNA-seq and scRNA-seq analysis of the DEGs in the IMQ group and WT group (bulk RNA-seq data source GSM2299980 (WT), GSM2299981 (WT), GSM229998 (IMQ), GSM2299983 (IMQ)). F Violin plots showing the top DEGs between IMQ and WT in all fibroblast subtypes. G KEGG pathway map of IMQ vs. WT scRNA-seq in FBs. H Western blot image of Vim, Lcn2 and IL-17 expressions in HaCaT cells treated with IL-17 for indicated time. I – K Histogram of protein expression level in H . L – N Quantification of Vim, Lcn2 and IL-17 mRNA expressions in <t>NHDF</t> cells stimulated with IL-17 (20 ng/mL) for 6 h.
    Culture Conditions Normal Human Dermal Fibroblasts Nhdf, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC cxcl1 inhibition assay normal human dermal fibroblasts nhdf
    A UMAP plot of 33,118 FBs coloured by cell subtype. Each dot denotes a single cell. B Heatmap showing differences in hallmark pathway activities scored per cell with GSVA among different <t>fibroblast</t> subtypes. ( C ) Dot plot of the expression levels of marker genes for FB subtypes defined in A . Dot size corresponds to the percent of expressing cells, and dot colour indicates the average expression levels. D UMAP plot of all fibroblast subtypes from the skin of the IMQ group and WT group, coloured by cell type. E Bulk RNA-seq and scRNA-seq analysis of the DEGs in the IMQ group and WT group (bulk RNA-seq data source GSM2299980 (WT), GSM2299981 (WT), GSM229998 (IMQ), GSM2299983 (IMQ)). F Violin plots showing the top DEGs between IMQ and WT in all fibroblast subtypes. G KEGG pathway map of IMQ vs. WT scRNA-seq in FBs. H Western blot image of Vim, Lcn2 and IL-17 expressions in HaCaT cells treated with IL-17 for indicated time. I – K Histogram of protein expression level in H . L – N Quantification of Vim, Lcn2 and IL-17 mRNA expressions in <t>NHDF</t> cells stimulated with IL-17 (20 ng/mL) for 6 h.
    Cxcl1 Inhibition Assay Normal Human Dermal Fibroblasts Nhdf, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC normal human dermal fibroblast adult nhdf ad cell lines
    A UMAP plot of 33,118 FBs coloured by cell subtype. Each dot denotes a single cell. B Heatmap showing differences in hallmark pathway activities scored per cell with GSVA among different <t>fibroblast</t> subtypes. ( C ) Dot plot of the expression levels of marker genes for FB subtypes defined in A . Dot size corresponds to the percent of expressing cells, and dot colour indicates the average expression levels. D UMAP plot of all fibroblast subtypes from the skin of the IMQ group and WT group, coloured by cell type. E Bulk RNA-seq and scRNA-seq analysis of the DEGs in the IMQ group and WT group (bulk RNA-seq data source GSM2299980 (WT), GSM2299981 (WT), GSM229998 (IMQ), GSM2299983 (IMQ)). F Violin plots showing the top DEGs between IMQ and WT in all fibroblast subtypes. G KEGG pathway map of IMQ vs. WT scRNA-seq in FBs. H Western blot image of Vim, Lcn2 and IL-17 expressions in HaCaT cells treated with IL-17 for indicated time. I – K Histogram of protein expression level in H . L – N Quantification of Vim, Lcn2 and IL-17 mRNA expressions in <t>NHDF</t> cells stimulated with IL-17 (20 ng/mL) for 6 h.
    Normal Human Dermal Fibroblast Adult Nhdf Ad Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Cell viability and wound-healing assays. Notes: PPF leaves and stem picture. ( A ) PPF extract applied at 1 and 10 μg/mL showed no cytotoxicity in the EXCyto assay using NHDF cells. ( B ) The cell wound-healing assay was imaged 24 hours after scratching the cell monolayer and treating with PPF extract at the indicated doses. ( C ) Once cells reached confluence, a single wound was made in the center of the monolayer using a 10 μl pipette tip and the cells were treated with the indicated concentration of PPF extracts. After a 24-hour incubation, a photograph was taken at ×10 magnification under a microscope. ( D ) The photographic images from ( C ) in a dose-series were analyzed for gap area. The results are presented as the mean ± standard deviation of three independent experiments. ** P <0.01. Abbreviations: Conc, concentration; NHDF, normal human dermal fibroblast; PPF , Piper cambodianum P. Fourn.

    Journal: Clinical Interventions in Aging

    Article Title: Anti-aging effects of Piper cambodianum P. Fourn. extract on normal human dermal fibroblast cells and a wound-healing model in mice

    doi: 10.2147/CIA.S107734

    Figure Lengend Snippet: Cell viability and wound-healing assays. Notes: PPF leaves and stem picture. ( A ) PPF extract applied at 1 and 10 μg/mL showed no cytotoxicity in the EXCyto assay using NHDF cells. ( B ) The cell wound-healing assay was imaged 24 hours after scratching the cell monolayer and treating with PPF extract at the indicated doses. ( C ) Once cells reached confluence, a single wound was made in the center of the monolayer using a 10 μl pipette tip and the cells were treated with the indicated concentration of PPF extracts. After a 24-hour incubation, a photograph was taken at ×10 magnification under a microscope. ( D ) The photographic images from ( C ) in a dose-series were analyzed for gap area. The results are presented as the mean ± standard deviation of three independent experiments. ** P <0.01. Abbreviations: Conc, concentration; NHDF, normal human dermal fibroblast; PPF , Piper cambodianum P. Fourn.

    Article Snippet: Normal Adult Human Primary Dermal Fibroblasts (NHDF) cells were purchased from ATCC (PCS-201-012, Manassas, VA, USA).

    Techniques: Wound Healing Assay, Transferring, Concentration Assay, Incubation, Microscopy, Standard Deviation

    ECM gene expression level by q-PCR. Notes: RNAs from untreated or PPF -treated cells were analyzed by real-time q-PCR with ( A ) collagen-, ( B ) elastin-, ( C ) hyaluronan synthase (HAS)-2, and ( D ) matrix metalloproteinase (MMP)-1-specific primers. The results are presented as the mean ± SD of three independent experiments. * P <0.05, ** P <0.01. Abbreviations: Conc, concentration; ECM, extracellular matrix; HAS, hyaluronan synthase; MMP, matrix metalloproteinase; mRNA, messenger RNA; NHDF, normal human dermal fibroblast; PPF , Piper cambodianum P. Fourn.; q-PCR, quantitative polymerase chain reaction; RNA, ribonucleic acid; SD, standard deviation.

    Journal: Clinical Interventions in Aging

    Article Title: Anti-aging effects of Piper cambodianum P. Fourn. extract on normal human dermal fibroblast cells and a wound-healing model in mice

    doi: 10.2147/CIA.S107734

    Figure Lengend Snippet: ECM gene expression level by q-PCR. Notes: RNAs from untreated or PPF -treated cells were analyzed by real-time q-PCR with ( A ) collagen-, ( B ) elastin-, ( C ) hyaluronan synthase (HAS)-2, and ( D ) matrix metalloproteinase (MMP)-1-specific primers. The results are presented as the mean ± SD of three independent experiments. * P <0.05, ** P <0.01. Abbreviations: Conc, concentration; ECM, extracellular matrix; HAS, hyaluronan synthase; MMP, matrix metalloproteinase; mRNA, messenger RNA; NHDF, normal human dermal fibroblast; PPF , Piper cambodianum P. Fourn.; q-PCR, quantitative polymerase chain reaction; RNA, ribonucleic acid; SD, standard deviation.

    Article Snippet: Normal Adult Human Primary Dermal Fibroblasts (NHDF) cells were purchased from ATCC (PCS-201-012, Manassas, VA, USA).

    Techniques: Expressing, Concentration Assay, Real-time Polymerase Chain Reaction, Standard Deviation

    Regulation of ECM proteins in PPF -treated cells. Notes: ( A ) NHDF cells were treated with PPF for 24 hours. Cell lysates were analyzed by immunoblotting using anti-collagen, anti-elastin, anti-MMP-3, and anti-ERK-2 antibodies. Results are representative of three independent experiments. ( B ) Actin, collagen, elastin, MMP-3, and ERK-2 in the immunoprecipitates were quantified by Western blotting. Bar heights are the respective mean ± SDs of three independent experiments. * P <0.05; ** P <0.01. Abbreviations: Conc, concentration; ECM, extracellular matrix; ERK, extracellular signal-regulated kinase; MMP, matrix metalloproteinase; NHDF, normal human dermal fibroblast; PPF , Piper cambodianum P. Fourn.; SDs, standard deviations.

    Journal: Clinical Interventions in Aging

    Article Title: Anti-aging effects of Piper cambodianum P. Fourn. extract on normal human dermal fibroblast cells and a wound-healing model in mice

    doi: 10.2147/CIA.S107734

    Figure Lengend Snippet: Regulation of ECM proteins in PPF -treated cells. Notes: ( A ) NHDF cells were treated with PPF for 24 hours. Cell lysates were analyzed by immunoblotting using anti-collagen, anti-elastin, anti-MMP-3, and anti-ERK-2 antibodies. Results are representative of three independent experiments. ( B ) Actin, collagen, elastin, MMP-3, and ERK-2 in the immunoprecipitates were quantified by Western blotting. Bar heights are the respective mean ± SDs of three independent experiments. * P <0.05; ** P <0.01. Abbreviations: Conc, concentration; ECM, extracellular matrix; ERK, extracellular signal-regulated kinase; MMP, matrix metalloproteinase; NHDF, normal human dermal fibroblast; PPF , Piper cambodianum P. Fourn.; SDs, standard deviations.

    Article Snippet: Normal Adult Human Primary Dermal Fibroblasts (NHDF) cells were purchased from ATCC (PCS-201-012, Manassas, VA, USA).

    Techniques: Western Blot, Concentration Assay

    PPF extract inhibition of ROS production in UV-irradiated cells. Notes: ( A ) NHDF cells were exposed to UV irradiation at 40 J for 180 seconds and treated with PPF for 24 hours. Cell lysates were analyzed by immunoblotting using anti-TNFR6, anti-c-Jun, anti-c-Fos, anti-pp38, or anti-p-JNK antibodies. Results are representative of three independent experiments. ( B ) Total and tyrosine-phosphorylated p38, JUK, actin, and TNFR6 in the immunoprecipitates were quantified by Western analyses. Bar heights are mean ± SD of three independent experiments. * P <0.05; ** P <0.01. Abbreviations: Conc, concentration; EGFR, epidermal growth factor receptor; JNK, c-Jun amino terminal kinase; JNK, c-Jun N-terminal kinase; NHDF, normal human dermal fibroblast; PPF , Piper cambodianum P. Fourn.; ROS, reactive oxygen species; SD, standard deviation; TNFR, tumor necrosis factor receptor; UV, ultraviolet.

    Journal: Clinical Interventions in Aging

    Article Title: Anti-aging effects of Piper cambodianum P. Fourn. extract on normal human dermal fibroblast cells and a wound-healing model in mice

    doi: 10.2147/CIA.S107734

    Figure Lengend Snippet: PPF extract inhibition of ROS production in UV-irradiated cells. Notes: ( A ) NHDF cells were exposed to UV irradiation at 40 J for 180 seconds and treated with PPF for 24 hours. Cell lysates were analyzed by immunoblotting using anti-TNFR6, anti-c-Jun, anti-c-Fos, anti-pp38, or anti-p-JNK antibodies. Results are representative of three independent experiments. ( B ) Total and tyrosine-phosphorylated p38, JUK, actin, and TNFR6 in the immunoprecipitates were quantified by Western analyses. Bar heights are mean ± SD of three independent experiments. * P <0.05; ** P <0.01. Abbreviations: Conc, concentration; EGFR, epidermal growth factor receptor; JNK, c-Jun amino terminal kinase; JNK, c-Jun N-terminal kinase; NHDF, normal human dermal fibroblast; PPF , Piper cambodianum P. Fourn.; ROS, reactive oxygen species; SD, standard deviation; TNFR, tumor necrosis factor receptor; UV, ultraviolet.

    Article Snippet: Normal Adult Human Primary Dermal Fibroblasts (NHDF) cells were purchased from ATCC (PCS-201-012, Manassas, VA, USA).

    Techniques: Inhibition, Irradiation, Western Blot, Concentration Assay, Standard Deviation

    PPF -mediated inhibition of ROS production in UV-irradiated cells. Notes: ( A ) Cells were treated with UVB irradiation (40 J) for the indicated time and then incubated with 2′,7′-dichlorodihydrofluorescein diacetate for 30 minutes. The generation of ROS was measured by FACS. ( B ) Cells were exposed to UVB (40 J) for 180 seconds and treated with the indicated concentration of PPF for 24 hours. ROS production were estimated with the staining of 2′,7′-dichlorodihydrofluorescein diacetate and analyzed by FACS. Abbreviations: FACS, fluorescence-activated cell sorting; FITC, fluorescein isothiocyanate; NHDF, normal human dermal fibroblast; PPF , Piper cambodianum P. Fourn.; ROS, reactive oxygen species; UV, ultraviolet; UVB, ultraviolet B.

    Journal: Clinical Interventions in Aging

    Article Title: Anti-aging effects of Piper cambodianum P. Fourn. extract on normal human dermal fibroblast cells and a wound-healing model in mice

    doi: 10.2147/CIA.S107734

    Figure Lengend Snippet: PPF -mediated inhibition of ROS production in UV-irradiated cells. Notes: ( A ) Cells were treated with UVB irradiation (40 J) for the indicated time and then incubated with 2′,7′-dichlorodihydrofluorescein diacetate for 30 minutes. The generation of ROS was measured by FACS. ( B ) Cells were exposed to UVB (40 J) for 180 seconds and treated with the indicated concentration of PPF for 24 hours. ROS production were estimated with the staining of 2′,7′-dichlorodihydrofluorescein diacetate and analyzed by FACS. Abbreviations: FACS, fluorescence-activated cell sorting; FITC, fluorescein isothiocyanate; NHDF, normal human dermal fibroblast; PPF , Piper cambodianum P. Fourn.; ROS, reactive oxygen species; UV, ultraviolet; UVB, ultraviolet B.

    Article Snippet: Normal Adult Human Primary Dermal Fibroblasts (NHDF) cells were purchased from ATCC (PCS-201-012, Manassas, VA, USA).

    Techniques: Inhibition, Irradiation, Incubation, Concentration Assay, Staining, Fluorescence, FACS

    A UMAP plot of 33,118 FBs coloured by cell subtype. Each dot denotes a single cell. B Heatmap showing differences in hallmark pathway activities scored per cell with GSVA among different fibroblast subtypes. ( C ) Dot plot of the expression levels of marker genes for FB subtypes defined in A . Dot size corresponds to the percent of expressing cells, and dot colour indicates the average expression levels. D UMAP plot of all fibroblast subtypes from the skin of the IMQ group and WT group, coloured by cell type. E Bulk RNA-seq and scRNA-seq analysis of the DEGs in the IMQ group and WT group (bulk RNA-seq data source GSM2299980 (WT), GSM2299981 (WT), GSM229998 (IMQ), GSM2299983 (IMQ)). F Violin plots showing the top DEGs between IMQ and WT in all fibroblast subtypes. G KEGG pathway map of IMQ vs. WT scRNA-seq in FBs. H Western blot image of Vim, Lcn2 and IL-17 expressions in HaCaT cells treated with IL-17 for indicated time. I – K Histogram of protein expression level in H . L – N Quantification of Vim, Lcn2 and IL-17 mRNA expressions in NHDF cells stimulated with IL-17 (20 ng/mL) for 6 h.

    Journal: Cell Death & Disease

    Article Title: Single-cell RNA-seq reveals keratinocyte and fibroblast heterogeneity and their crosstalk via epithelial-mesenchymal transition in psoriasis

    doi: 10.1038/s41419-024-06583-z

    Figure Lengend Snippet: A UMAP plot of 33,118 FBs coloured by cell subtype. Each dot denotes a single cell. B Heatmap showing differences in hallmark pathway activities scored per cell with GSVA among different fibroblast subtypes. ( C ) Dot plot of the expression levels of marker genes for FB subtypes defined in A . Dot size corresponds to the percent of expressing cells, and dot colour indicates the average expression levels. D UMAP plot of all fibroblast subtypes from the skin of the IMQ group and WT group, coloured by cell type. E Bulk RNA-seq and scRNA-seq analysis of the DEGs in the IMQ group and WT group (bulk RNA-seq data source GSM2299980 (WT), GSM2299981 (WT), GSM229998 (IMQ), GSM2299983 (IMQ)). F Violin plots showing the top DEGs between IMQ and WT in all fibroblast subtypes. G KEGG pathway map of IMQ vs. WT scRNA-seq in FBs. H Western blot image of Vim, Lcn2 and IL-17 expressions in HaCaT cells treated with IL-17 for indicated time. I – K Histogram of protein expression level in H . L – N Quantification of Vim, Lcn2 and IL-17 mRNA expressions in NHDF cells stimulated with IL-17 (20 ng/mL) for 6 h.

    Article Snippet: Normal human dermal fibroblast (NHDF) was cultured and expanded in Fibroblast Basal Medium (PCS-201-030, ATCC, U.S.A) supplemented with Fibroblast Growth Kit-Low serum (PCS-201-041, ATCC, U.S.A) and 1% penicillin-streptomycin.

    Techniques: Expressing, Marker, RNA Sequencing Assay, Western Blot