nonidet p40  (Millipore)


Bioz Verified Symbol Millipore is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Name:
    Nonidet P 40
    Description:

    Catalog Number:
    21-3277
    Price:
    None
    Buy from Supplier


    Structured Review

    Millipore nonidet p40
    Nonidet P 40

    https://www.bioz.com/result/nonidet p40/product/Millipore
    Average 99 stars, based on 192 article reviews
    Price from $9.99 to $1999.99
    nonidet p40 - by Bioz Stars, 2020-11
    99/100 stars

    Images

    Related Articles

    Stable Transfection:

    Article Title: SIVA1 directs the E3 ubiquitin ligase RAD18 for PCNA monoubiquitination
    Article Snippet: .. For affinity purification, HEK293T cells stably expressing tagged proteins were lysed with NETN buffer (20 mM Tris-HCl, pH 8.0, 100 mM NaCl, 1 mM EDTA, and 0.5% Nonidet P-40) containing Benzonase (EMD Millipore) for 20 min. .. The supernatants were cleared at 14,000 rpm to remove debris and then incubated with streptavidin-conjugated beads (GE Healthcare) for 2 h at 4°C.

    Protease Inhibitor:

    Article Title: The Epstein-Barr Virus G-Protein-Coupled Receptor Contributes to Immune Evasion by Targeting MHC Class I Molecules for Degradation
    Article Snippet: .. Samples containing 2×106 cells were lysed in 400 µl of NP-40 buffer (0.5% Nonidet P-40, 5 mM MgCl2 and 50 mM Tris-HCl, pH 7.5) with protease inhibitor cocktail (Sigma) at 4°C for 45 min. .. Nuclei and insoluble debris were removed by centrifugation, and the supernatants were precleared first with 1.2 µl of normal mouse serum and 20 µl Dynabeads Protein A (Invitrogen) for 2 hr at 4°C, and then again with 20 µl Dynabeads Protein A and 20 µl Dynabeads protein G at 4°C overnight.

    other:

    Article Title: Oda5p, a Novel Axonemal Protein Required for Assembly of the Outer Dynein Arm and an Associated Adenylate Kinase D⃞
    Article Snippet: Because the AK is not predicted to contain transmembrane domains, and because its presence in the flagellum is dependent upon Oda5p, which is an axonemal protein, it is probable that the AK also is an axonemal component and that it is associated with the axoneme via interactions that survive the Tergitol treatment but are disrupted by Nonidet P-40.

    Article Title: Recruitment of the cross-linked opsonic receptor CD32A (Fc?RIIA) to high-density detergent-resistant membrane domains in human neutrophils
    Article Snippet: iPr2 P -F (di-isopropyl fluorophosphate) was obtained from Helixx Technologies (Scarborough, ON, Canada) and NP40 (Nonidet P40) and 4-amino-5-(4-chlorophenyl)-7-(t-butyl)pyrazolo[3,4- d ]pyrimidine (referred to as PP2) were obtained from Calbiochem (San Diego, CA, U.S.A.).

    Article Title: Krp1 (Sarcosin) Promotes Lateral Fusion of Myofibril Assembly Intermediates in Cultured Mouse Cardiomyocytes
    Article Snippet: Krp1 association with cellular compartments was further explored by separation of extracted and insoluble fractions following treatment with Nonidet P-40.

    Expressing:

    Article Title: SIVA1 directs the E3 ubiquitin ligase RAD18 for PCNA monoubiquitination
    Article Snippet: .. For affinity purification, HEK293T cells stably expressing tagged proteins were lysed with NETN buffer (20 mM Tris-HCl, pH 8.0, 100 mM NaCl, 1 mM EDTA, and 0.5% Nonidet P-40) containing Benzonase (EMD Millipore) for 20 min. .. The supernatants were cleared at 14,000 rpm to remove debris and then incubated with streptavidin-conjugated beads (GE Healthcare) for 2 h at 4°C.

    Isolation:

    Article Title: Novel Filaments 5 nm in Diameter Constitute the Cytosolic Ring of the Plastid Division Apparatus
    Article Snippet: .. Dividing and nondividing chloroplasts were lysed, and protein concentrations were normalized (1 mg/mL) in sucrose-free isolation medium containing 0.5% Nonidet P-40 and 100 μg/mL DNase I (DN-25; Sigma) for 1 hr on ice. ..

    Lysis:

    Article Title: The Modulation of CD40 Ligand Signaling by Transmembrane CD28 Splice Variant in Human T Cells
    Article Snippet: .. Cell extracts were prepared using lysis buffer containing 1% Nonidet P-40 (BDH Chemicals) or 1% digitonin (Sigma-Aldrich; reference ). .. To immunoprecipitate CD28i-HA with anti-HA Ab (rat IgG1), protein A/G agarose and protein L agarose bead cocktail (sc-2336; Santa Cruz Biotechnology, Inc.) were used.

    Affinity Purification:

    Article Title: SIVA1 directs the E3 ubiquitin ligase RAD18 for PCNA monoubiquitination
    Article Snippet: .. For affinity purification, HEK293T cells stably expressing tagged proteins were lysed with NETN buffer (20 mM Tris-HCl, pH 8.0, 100 mM NaCl, 1 mM EDTA, and 0.5% Nonidet P-40) containing Benzonase (EMD Millipore) for 20 min. .. The supernatants were cleared at 14,000 rpm to remove debris and then incubated with streptavidin-conjugated beads (GE Healthcare) for 2 h at 4°C.

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Millipore np40
    Cross-linking of CD32A in isolated plasma membranes induces its recruitment to high-density DRMs CD32A was cross-linked on neutrophil membranes as described in the Materials and methods section. After <t>NP40</t> solubilization, the membranes were subjected to ultracentrifugation on Optiprep gradients. Then, 13 fractions were collected and the proteins were precipitated and analysed by SDS/PAGE. ( A ) Membranes were immunoblotted with a CT10 (anti-CD32A) or anti-flotillin-1 antibody. ( B ) Membranes were immunoblotted with an anti-pY antibody.
    Np40, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 369 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/np40/product/Millipore
    Average 99 stars, based on 369 article reviews
    Price from $9.99 to $1999.99
    np40 - by Bioz Stars, 2020-11
    99/100 stars
      Buy from Supplier

    Image Search Results


    Cross-linking of CD32A in isolated plasma membranes induces its recruitment to high-density DRMs CD32A was cross-linked on neutrophil membranes as described in the Materials and methods section. After NP40 solubilization, the membranes were subjected to ultracentrifugation on Optiprep gradients. Then, 13 fractions were collected and the proteins were precipitated and analysed by SDS/PAGE. ( A ) Membranes were immunoblotted with a CT10 (anti-CD32A) or anti-flotillin-1 antibody. ( B ) Membranes were immunoblotted with an anti-pY antibody.

    Journal: Biochemical Journal

    Article Title: Recruitment of the cross-linked opsonic receptor CD32A (Fc?RIIA) to high-density detergent-resistant membrane domains in human neutrophils

    doi: 10.1042/BJ20031808

    Figure Lengend Snippet: Cross-linking of CD32A in isolated plasma membranes induces its recruitment to high-density DRMs CD32A was cross-linked on neutrophil membranes as described in the Materials and methods section. After NP40 solubilization, the membranes were subjected to ultracentrifugation on Optiprep gradients. Then, 13 fractions were collected and the proteins were precipitated and analysed by SDS/PAGE. ( A ) Membranes were immunoblotted with a CT10 (anti-CD32A) or anti-flotillin-1 antibody. ( B ) Membranes were immunoblotted with an anti-pY antibody.

    Article Snippet: iPr2 P -F (di-isopropyl fluorophosphate) was obtained from Helixx Technologies (Scarborough, ON, Canada) and NP40 (Nonidet P40) and 4-amino-5-(4-chlorophenyl)-7-(t-butyl)pyrazolo[3,4- d ]pyrimidine (referred to as PP2) were obtained from Calbiochem (San Diego, CA, U.S.A.).

    Techniques: Isolation, SDS Page

    Solubilization profiles of CD32A and Src kinases on neutrophil membranes after cross-linking of CD32A CD32A was cross-linked on neutrophil membranes as described in the Materials and methods section. Membranes were then solubilized for 20 min in 1% NP40 on ice, 1% NP40 at 37 °C or 100 mM OGD on ice. After centrifugation (15000 g , 10 min), the supernatants (S) and pellets (P) were analysed by SDS/PAGE and probed for CD32A, Hck, Lyn or Fgr as described in the Materials and methods section. M, whole membranes before solubilization in detergents.

    Journal: Biochemical Journal

    Article Title: Recruitment of the cross-linked opsonic receptor CD32A (Fc?RIIA) to high-density detergent-resistant membrane domains in human neutrophils

    doi: 10.1042/BJ20031808

    Figure Lengend Snippet: Solubilization profiles of CD32A and Src kinases on neutrophil membranes after cross-linking of CD32A CD32A was cross-linked on neutrophil membranes as described in the Materials and methods section. Membranes were then solubilized for 20 min in 1% NP40 on ice, 1% NP40 at 37 °C or 100 mM OGD on ice. After centrifugation (15000 g , 10 min), the supernatants (S) and pellets (P) were analysed by SDS/PAGE and probed for CD32A, Hck, Lyn or Fgr as described in the Materials and methods section. M, whole membranes before solubilization in detergents.

    Article Snippet: iPr2 P -F (di-isopropyl fluorophosphate) was obtained from Helixx Technologies (Scarborough, ON, Canada) and NP40 (Nonidet P40) and 4-amino-5-(4-chlorophenyl)-7-(t-butyl)pyrazolo[3,4- d ]pyrimidine (referred to as PP2) were obtained from Calbiochem (San Diego, CA, U.S.A.).

    Techniques: Centrifugation, SDS Page

    Cross-linking-induced insolubility of CD32A in non-ionic detergents on neutrophil membranes CD32A was cross-linked on neutrophil membranes as described in the Materials and methods section and membranes were solubilized in 1% NP40 and centrifuged at 15000 g for 10 min ( A ) or at 100000 g for 1 h ( B ). The supernatant (S) and pellet (P) were subsequently analysed by SDS/PAGE and immunoblotted with an anti-pY or CT10 antibody (anti-CD32A). The two major substrates whose tyrosine phosphorylation is enhanced are indicated with arrows.

    Journal: Biochemical Journal

    Article Title: Recruitment of the cross-linked opsonic receptor CD32A (Fc?RIIA) to high-density detergent-resistant membrane domains in human neutrophils

    doi: 10.1042/BJ20031808

    Figure Lengend Snippet: Cross-linking-induced insolubility of CD32A in non-ionic detergents on neutrophil membranes CD32A was cross-linked on neutrophil membranes as described in the Materials and methods section and membranes were solubilized in 1% NP40 and centrifuged at 15000 g for 10 min ( A ) or at 100000 g for 1 h ( B ). The supernatant (S) and pellet (P) were subsequently analysed by SDS/PAGE and immunoblotted with an anti-pY or CT10 antibody (anti-CD32A). The two major substrates whose tyrosine phosphorylation is enhanced are indicated with arrows.

    Article Snippet: iPr2 P -F (di-isopropyl fluorophosphate) was obtained from Helixx Technologies (Scarborough, ON, Canada) and NP40 (Nonidet P40) and 4-amino-5-(4-chlorophenyl)-7-(t-butyl)pyrazolo[3,4- d ]pyrimidine (referred to as PP2) were obtained from Calbiochem (San Diego, CA, U.S.A.).

    Techniques: SDS Page

    Cross-linking-induced insolubility of CD32A occurs in the absence of tyrosine phosphorylation CD32A was cross-linked on neutrophil membranes in the presence (+) or absence (−) of the kinase mixture (KM) and VO 4 . Membranes were solubilized with 1% NP40 and after centrifugation (15000 g , 10 min), the supernatants (S) and pellets (P) were probed for an anti-pY or anti-CD32A antibody as described in the Materials and methods section.

    Journal: Biochemical Journal

    Article Title: Recruitment of the cross-linked opsonic receptor CD32A (Fc?RIIA) to high-density detergent-resistant membrane domains in human neutrophils

    doi: 10.1042/BJ20031808

    Figure Lengend Snippet: Cross-linking-induced insolubility of CD32A occurs in the absence of tyrosine phosphorylation CD32A was cross-linked on neutrophil membranes in the presence (+) or absence (−) of the kinase mixture (KM) and VO 4 . Membranes were solubilized with 1% NP40 and after centrifugation (15000 g , 10 min), the supernatants (S) and pellets (P) were probed for an anti-pY or anti-CD32A antibody as described in the Materials and methods section.

    Article Snippet: iPr2 P -F (di-isopropyl fluorophosphate) was obtained from Helixx Technologies (Scarborough, ON, Canada) and NP40 (Nonidet P40) and 4-amino-5-(4-chlorophenyl)-7-(t-butyl)pyrazolo[3,4- d ]pyrimidine (referred to as PP2) were obtained from Calbiochem (San Diego, CA, U.S.A.).

    Techniques: Centrifugation