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Bio-Rad nonidet p 40
Nonidet P 40, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/nonidet p 40/product/Bio-Rad
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
nonidet p 40 - by Bioz Stars, 2021-05
86/100 stars

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Related Articles

Western Blot:

Article Title: Characterization of immune responses following intramuscular DNA immunization with the MOMP gene of Chlamydia trachomatis mouse pneumonitis strain
Article Snippet: The plates were read with a microplate reader at 405 nm. .. Western blot analysis was performed to identify anti-MOMP antibody in the sera of immunized mice., Briefly, purified MoPn EBs were lysed by boiling in sample buffer containing 2% sodium dodecyl sulphate (SDS) and 200 mm 2ME and subsequently electrophoresed in a 10% SDS–polyacrylamide gel (Bio-Rad, Hercules, CA). ..

Article Title: Novel ACE2-IgG1 fusions with improved in vitro and in vivo activity against SARS-CoV2
Article Snippet: BCA assay was performed to quantify protein and 5.0 μg protein was used for Western blotting. .. Western blots were performed using 7.5% SDS-PAGE gels (Bio-Rad) under the non-reducing or reducing condition with 2.5% 2-mercaptoethanol and transferred to PVDF membranes. .. The blot was probed with goat anti-human IgG-HRP (Southern Biotech).

Mouse Assay:

Article Title: Characterization of immune responses following intramuscular DNA immunization with the MOMP gene of Chlamydia trachomatis mouse pneumonitis strain
Article Snippet: The plates were read with a microplate reader at 405 nm. .. Western blot analysis was performed to identify anti-MOMP antibody in the sera of immunized mice., Briefly, purified MoPn EBs were lysed by boiling in sample buffer containing 2% sodium dodecyl sulphate (SDS) and 200 mm 2ME and subsequently electrophoresed in a 10% SDS–polyacrylamide gel (Bio-Rad, Hercules, CA). ..

Purification:

Article Title: Characterization of immune responses following intramuscular DNA immunization with the MOMP gene of Chlamydia trachomatis mouse pneumonitis strain
Article Snippet: The plates were read with a microplate reader at 405 nm. .. Western blot analysis was performed to identify anti-MOMP antibody in the sera of immunized mice., Briefly, purified MoPn EBs were lysed by boiling in sample buffer containing 2% sodium dodecyl sulphate (SDS) and 200 mm 2ME and subsequently electrophoresed in a 10% SDS–polyacrylamide gel (Bio-Rad, Hercules, CA). ..

SDS Page:

Article Title: The Membrane M Protein Carboxy Terminus Binds to Transmissible Gastroenteritis Coronavirus Core and Contributes to Core Stability
Article Snippet: .. The M-to-N molar ratio was estimated after KCl, NaCl, guanidine isothiocyanate, Triton X-100, and 2-mercaptoethanol treatments by SDS-PAGE, silver staining , and band densitometry using a Gel Documentation System 2000 (Bio-Rad). .. For Western blot analysis, the proteins were transferred to a nitrocellulose membrane with a Bio-Rad Mini Protean II electroblotting apparatus at 150 mA for 2 h in 25 mM Tris–192 mM glycine buffer (pH 8.3) containing 20% methanol.

Article Title: Novel ACE2-IgG1 fusions with improved in vitro and in vivo activity against SARS-CoV2
Article Snippet: BCA assay was performed to quantify protein and 5.0 μg protein was used for Western blotting. .. Western blots were performed using 7.5% SDS-PAGE gels (Bio-Rad) under the non-reducing or reducing condition with 2.5% 2-mercaptoethanol and transferred to PVDF membranes. .. The blot was probed with goat anti-human IgG-HRP (Southern Biotech).

Article Title: Gamma-interferon-inducible, lysosome/endosome-localized thiolreductase, GILT, has anti-retroviral activity and its expression is counteracted by HIV-1
Article Snippet: Reducing and non-reducing SDS-PAGE, and western immunoblottingCell lysates were prepared with lysis buffer containing 2 mM N-ethylmaleimide (NEM) [ ]. .. The cell lysates were mixed with 2x sample buffer with or without 10 % 2-mercaptoethanol (2-ME) (Wako), and were subjected to SDS-PAGE (BioRad) with or without 2-ME. .. The fractionated proteins were transferred onto a PVDF membrane (Millipore).

Article Title: Drosophila vitelline membrane assembly: A critical role for an evolutionarily conserved cysteine in the \u201cVM domain\u201d of sV23
Article Snippet: .. Proteins soluble in Laemmli sample containing 5% beta-mercaptoethanol were separated by SDS-PAGE and transferred to nitrocellulose using a Bio-Rad Mini-Protean system. .. ECL Western blot signals were developed as previously described ( ).

Silver Staining:

Article Title: The Membrane M Protein Carboxy Terminus Binds to Transmissible Gastroenteritis Coronavirus Core and Contributes to Core Stability
Article Snippet: .. The M-to-N molar ratio was estimated after KCl, NaCl, guanidine isothiocyanate, Triton X-100, and 2-mercaptoethanol treatments by SDS-PAGE, silver staining , and band densitometry using a Gel Documentation System 2000 (Bio-Rad). .. For Western blot analysis, the proteins were transferred to a nitrocellulose membrane with a Bio-Rad Mini Protean II electroblotting apparatus at 150 mA for 2 h in 25 mM Tris–192 mM glycine buffer (pH 8.3) containing 20% methanol.

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    Bio-Rad nonidet p 40
    Nonidet P 40, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nonidet p 40/product/Bio-Rad
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    nonidet p 40 - by Bioz Stars, 2021-05
    86/100 stars
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    99
    Bio-Rad lysis buffer
    Lysis Buffer, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bio-Rad salt buffer
    Salt Buffer, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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