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noncontact sciflexarrayer s12 microarray spotter  (SCIENION)


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    SCIENION noncontact sciflexarrayer s12 microarray spotter
    Noncontact Sciflexarrayer S12 Microarray Spotter, supplied by SCIENION, used in various techniques. Bioz Stars score: 95/100, based on 76 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/noncontact+microarray+spotter/sciFLEXARRAYER+S12/pmc12501125-155-35-40
    Average 95 stars, based on 76 article reviews
    noncontact sciflexarrayer s12 microarray spotter - by Bioz Stars, 2026-10
    95/100 stars

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    Related Articles

    Microarray:

    Article Title: EV Separation: Release of Intact Extracellular Vesicles Immunocaptured on Magnetic Particles
    Article Snippet: Extracellular vesicles (EVs) have attracted considerable interest due to their role in cell−cell communication, disease diagnosis, and drug delivery.. Despite their potential in the medical field, there is no consensus on the best method for separating microand nanovesicles from cell culture supernatant and complex biological fluids.. Obtaining a good recovery yield and preserving physical characteristics is critical for the diagnostic and therapeutic use of EVs.

    Article Title: A Bi-Functional Polymeric Coating for the Co-Imobilization of Proteins and Peptides on Microarray Substrates
    Article Snippet: .. The solutions of COCU8 NH2 and COCU8 DBCO were deposited onto the surface of the same chip using a noncontact microarray spotter (Scienion sciFLEXARRAYER S12) using a 80 μm nozzle (see scheme in Figure 2). ..

    Article Title: Composite Peptide-Agarose Hydrogels for Robust and High-Sensitivity 3D Immunoassays.
    Article Snippet: Canonical immunoassays rely on highly sensitive and specific capturing of circulating biomarkers by interacting biomolecular baits.. In this frame, bioprobe immobilization in spatially discrete three-dimensional (3D) spots onto analytical surfaces by hydrogel encapsulation was shown to provide relevant advantages over conventional two-dimensional (2D) platforms.. Yet, the broad application of 3D systems is still hampered by hurdles in matching their straightforward fabrication with optimal functional properties.

    Article Title: Separation of Extracellular Vesicles by DNA-Directed Immunocapturing Followed by Enzymatic Release
    Article Snippet: .. Chips were spotted using a noncontact microarray spotter (sciFLEXARRAYER S12, Scienion, Berlin) equipped with an 80 μm nozzle. ..

    Article Title: Copolymer-Coated Gold Nanoparticles: Enhanced Stability and Customizable Functionalization for Biological Assays
    Article Snippet: .. Supports were spotted using a noncontact microarray spotter (sciFLEXARRAYER S12, Scienion, Berlin) equipped with an 80 μm nozzle. ..

    Article Title: Immobilization of biotinylated antibodies through streptavidin binding aptamer.
    Article Snippet: Liquid biopsy approaches are powerful strategies that potentially allow the diagnosis and prognosis of a number of diseases.. The field is continuously and rapidly growing, encouraging the discovery of novel predictory biomarkers.. Antibodies are usually exploited in sensors to validate biomarker candidates.

    Article Title: Advantageous antibody microarray fabrication through DNA-directed immobilization: A step toward use of extracellular vesicles in diagnostics
    Article Snippet: Microarrays were introduced to run multiple assays on a single platform.. Since then, researchers developed DNA and protein microarrays to study both transcription and expression of genes.. Protein microarray technology represents a powerful tool to get an insight into living systems.

    Concentration Assay:

    Article Title: Advantageous antibody microarray fabrication through DNA-directed immobilization: A step toward use of extracellular vesicles in diagnostics
    Article Snippet: Microarrays were introduced to run multiple assays on a single platform.. Since then, researchers developed DNA and protein microarrays to study both transcription and expression of genes.. Protein microarray technology represents a powerful tool to get an insight into living systems.



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    SCIENION noncontact sciflexarrayer s12 microarray spotter
    Noncontact Sciflexarrayer S12 Microarray Spotter, supplied by SCIENION, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Fig. 3. Relative Fluorescence Intensity measured on the spots after the interaction of fluorescently labeled secondary antibody with different AACs (Rabbit IgG-Tag1, Rabbit IgG-Tag2 and Rabbit IgG-Tag3) immobilized on a <t>microarray</t> functionalized with streptavidin (printed at a concentration of 2 mg/mL) and released after incubation with biotin. Blue bars reflect the AAC immobilized on streptavidin, yellow bars represent the AAC left on the spots after biotin-mediated release. Mean ± standard deviation of three independent measurements.
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    Fig. 3. Relative Fluorescence Intensity measured on the spots after the interaction of fluorescently labeled secondary antibody with different AACs (Rabbit IgG-Tag1, Rabbit IgG-Tag2 and Rabbit IgG-Tag3) immobilized on a <t>microarray</t> functionalized with streptavidin (printed at a concentration of 2 mg/mL) and released after incubation with biotin. Blue bars reflect the AAC immobilized on streptavidin, yellow bars represent the AAC left on the spots after biotin-mediated release. Mean ± standard deviation of three independent measurements.
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    SCIENION noncontact microarray spotter sciflexarrayer s12
    Fig. 3. Relative Fluorescence Intensity measured on the spots after the interaction of fluorescently labeled secondary antibody with different AACs (Rabbit IgG-Tag1, Rabbit IgG-Tag2 and Rabbit IgG-Tag3) immobilized on a <t>microarray</t> functionalized with streptavidin (printed at a concentration of 2 mg/mL) and released after incubation with biotin. Blue bars reflect the AAC immobilized on streptavidin, yellow bars represent the AAC left on the spots after biotin-mediated release. Mean ± standard deviation of three independent measurements.
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    Fig. 3. Relative Fluorescence Intensity measured on the spots after the interaction of fluorescently labeled secondary antibody with different AACs (Rabbit IgG-Tag1, Rabbit IgG-Tag2 and Rabbit IgG-Tag3) immobilized on a microarray functionalized with streptavidin (printed at a concentration of 2 mg/mL) and released after incubation with biotin. Blue bars reflect the AAC immobilized on streptavidin, yellow bars represent the AAC left on the spots after biotin-mediated release. Mean ± standard deviation of three independent measurements.

    Journal: Sensors and Actuators B: Chemical

    Article Title: Sequential separation and profiling of extracellular vesicles using antibody-aptamer conjugates

    doi: 10.1016/j.snb.2024.136939

    Figure Lengend Snippet: Fig. 3. Relative Fluorescence Intensity measured on the spots after the interaction of fluorescently labeled secondary antibody with different AACs (Rabbit IgG-Tag1, Rabbit IgG-Tag2 and Rabbit IgG-Tag3) immobilized on a microarray functionalized with streptavidin (printed at a concentration of 2 mg/mL) and released after incubation with biotin. Blue bars reflect the AAC immobilized on streptavidin, yellow bars represent the AAC left on the spots after biotin-mediated release. Mean ± standard deviation of three independent measurements.

    Article Snippet: Supports were spotted using a noncontact microarray spotter (sciFLEXARRAYER S12, Scienion, Berlin) equipped with an 80 μm nozzle.

    Techniques: Fluorescence, Labeling, Microarray, Concentration Assay, Incubation, Standard Deviation

    Fig. 4. Sequence-selective recapture on DNA microarray of 3 different AACs previously immobilized on streptavidin coated magnetic beads and released upon incubation with biotin. Bars represent the Relative Fluorescence Intensity measured on the spots after the interaction of fluorescently labeled secondary antibody with different AACs. Mean ± standard deviation of three independent measurements.

    Journal: Sensors and Actuators B: Chemical

    Article Title: Sequential separation and profiling of extracellular vesicles using antibody-aptamer conjugates

    doi: 10.1016/j.snb.2024.136939

    Figure Lengend Snippet: Fig. 4. Sequence-selective recapture on DNA microarray of 3 different AACs previously immobilized on streptavidin coated magnetic beads and released upon incubation with biotin. Bars represent the Relative Fluorescence Intensity measured on the spots after the interaction of fluorescently labeled secondary antibody with different AACs. Mean ± standard deviation of three independent measurements.

    Article Snippet: Supports were spotted using a noncontact microarray spotter (sciFLEXARRAYER S12, Scienion, Berlin) equipped with an 80 μm nozzle.

    Techniques: Sequencing, Microarray, Magnetic Beads, Incubation, Fluorescence, Labeling, Standard Deviation

    Fig. 5. Detection of EVs by label free interferometry separated and recaptured on DNA microarray using antiCD9-Tag2 (red bars). In the negative control, AAC- functionalized magnetic beads were incubated only with PBS-M (blue bars). Mean ± standard deviation of three independent measurements.

    Journal: Sensors and Actuators B: Chemical

    Article Title: Sequential separation and profiling of extracellular vesicles using antibody-aptamer conjugates

    doi: 10.1016/j.snb.2024.136939

    Figure Lengend Snippet: Fig. 5. Detection of EVs by label free interferometry separated and recaptured on DNA microarray using antiCD9-Tag2 (red bars). In the negative control, AAC- functionalized magnetic beads were incubated only with PBS-M (blue bars). Mean ± standard deviation of three independent measurements.

    Article Snippet: Supports were spotted using a noncontact microarray spotter (sciFLEXARRAYER S12, Scienion, Berlin) equipped with an 80 μm nozzle.

    Techniques: Microarray, Negative Control, Magnetic Beads, Incubation, Standard Deviation

    Fig. 6. Schematic representation of recapture experiment on ExoView R100 (upper panel) and relative high resolution close ups of a Probe2 spot throughout experimental stages (bottom panel). From left to the right it can be seen i) the surface of the DNA spot, ii) label free interferometry picture of EVs captured on the same spot, and iii) red and green fluorescence picture of the same spot after incubation with labeled antibodies. The bar graphs represent label free interferometric and fluorescent detection of EVs on DNA microarray after separation using streptavidin coated magnetic beads: (b) functionalized with antiCD9-Tag2; (c) func- tionalized with Rabbit IgG-Tag2; (d) non functionalized with AACs. As additional negative control, detection of plasma added with antiCD9-Tag2 without previous separation of EVs on magnetic beads (e). Mean ± standard deviation of three independent measurements.

    Journal: Sensors and Actuators B: Chemical

    Article Title: Sequential separation and profiling of extracellular vesicles using antibody-aptamer conjugates

    doi: 10.1016/j.snb.2024.136939

    Figure Lengend Snippet: Fig. 6. Schematic representation of recapture experiment on ExoView R100 (upper panel) and relative high resolution close ups of a Probe2 spot throughout experimental stages (bottom panel). From left to the right it can be seen i) the surface of the DNA spot, ii) label free interferometry picture of EVs captured on the same spot, and iii) red and green fluorescence picture of the same spot after incubation with labeled antibodies. The bar graphs represent label free interferometric and fluorescent detection of EVs on DNA microarray after separation using streptavidin coated magnetic beads: (b) functionalized with antiCD9-Tag2; (c) func- tionalized with Rabbit IgG-Tag2; (d) non functionalized with AACs. As additional negative control, detection of plasma added with antiCD9-Tag2 without previous separation of EVs on magnetic beads (e). Mean ± standard deviation of three independent measurements.

    Article Snippet: Supports were spotted using a noncontact microarray spotter (sciFLEXARRAYER S12, Scienion, Berlin) equipped with an 80 μm nozzle.

    Techniques: Fluorescence, Incubation, Labeling, Microarray, Magnetic Beads, Negative Control, Clinical Proteomics, Standard Deviation

    Fig. 8. Recapture and multiplexed detection on DNA microarray of EVs sepa- rated using antiCD9-Tag2 immobilized on magnetic beads and labeled using antiCD63-Tag1. Mean ± standard deviation of three independent measurements.

    Journal: Sensors and Actuators B: Chemical

    Article Title: Sequential separation and profiling of extracellular vesicles using antibody-aptamer conjugates

    doi: 10.1016/j.snb.2024.136939

    Figure Lengend Snippet: Fig. 8. Recapture and multiplexed detection on DNA microarray of EVs sepa- rated using antiCD9-Tag2 immobilized on magnetic beads and labeled using antiCD63-Tag1. Mean ± standard deviation of three independent measurements.

    Article Snippet: Supports were spotted using a noncontact microarray spotter (sciFLEXARRAYER S12, Scienion, Berlin) equipped with an 80 μm nozzle.

    Techniques: Microarray, Magnetic Beads, Labeling, Standard Deviation