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Journal: JACS Au
Article Title: Asymmetrical Biantennary Glycans Prepared by a Stop-and-Go Strategy Reveal Receptor Binding Evolution of Human Influenza A Viruses
doi: 10.1021/jacsau.3c00695
Figure Lengend Snippet: Probing receptor binding specificities of A(H3N2) and A(H1N1)pdm09. a) Collection of glycans printed on succinimide reactive microarray slides. Glycan binding data of b) early A(H3N2) and A(H3N2) 3C.2 viruses; c) A (H3N2) 3C.3 viruses; and d) A(H1N1)pdm09 viruses. Whole viruses were exposed to a glycan microarray, and binding was visualized antistalk antibodies. Bars represent the average relative fluorescence units (RFU) of four replicates ± SD.
Article Snippet: The glycans, which have an α-amine at the anomeric asparagine moiety, were printed on amine reactive NHS activated glass slides using a
Techniques: Binding Assay, Microarray, Glycoproteomics, Fluorescence