Journal: The FASEB Journal
Article Title: RNA stability protein ILF3 mediates cytokine-induced angiogenesis
Figure Lengend Snippet: ILF3 is expressed in angiogenic tissue and in response to angiogenic stimuli. A ) CD31 + vessels of ischemic porcine cardiac tissue. Representative immunohistochemical analysis of CD31 and ILF3 abundance in tissue stained with anti-CD31 or anti-ILF3 antibodies is shown. All sections were also stained with rabbit IgG negative control antibody. Original magnification, ×600. Scale bar, 100 μm. B ) ILF3 mRNA expression is induced in hCAECs in response to angiogenic cytokine treatment quantitated by qRT-PCR. C ) ILF3 protein is induced in response to angiogenic cytokines analyzed by Western blotting. Cells were treated with 100 ng/ml of IL-19 or 20 ng/ml of VEGF for 2, 4, 8, 16, or 24 h. D ) Densiometric analysis of ILF3 protein expression in hCAECs in response to angiogenic cytokine stimulation. * P
Article Snippet: In HeLa cells, ILF3 is considered to be predominantly nuclear, but becomes phosphorylated and translocates to the cytoplasm at the G2 /M boundary of the cell cycle ( ).
Techniques: Immunohistochemistry, Staining, Negative Control, Expressing, Quantitative RT-PCR, Western Blot