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Horizon Discovery non targeting dsrna
Non Targeting Dsrna, supplied by Horizon Discovery, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 88 stars, based on 1 article reviews
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non targeting dsrna - by Bioz Stars, 2020-08
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Article Title: 14-3-3 ? Expression Effects G2/M Response to Oxygen and Correlates with Ovarian Cancer Metastasis
Article Snippet: 14-3-3 σ siRNA and non-targeting dsRNA were purchased from Dharmacon.

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    Horizon Discovery ilf3
    <t>ILF3</t> is phosphorylated in response to angiogenic stimuli in hCAECs. A ) Representative Western blot of ILF3 phosphorylation in response to VEGF stimulation. B ) Densiometric analysis of ILF3 phosphorylation in response to VEGF stimulation. Phosphorylation is normalized to total ILF3 protein. C ) Representative Western blot of ILF3 phosphorylation in response to IL-19 stimulation. D ) Densiometric analysis of ILF3 phosphorylation in response to IL-19 stimulation. hCAEC lysates were separated on a 7.5% gradient phos-tag gel to measure ILF3 phosphorylation. Total ILF3 was normalized to HSC70, and phosphorylated-ILF3 was made relative to normalized total ILF3 using densitometry. * P
    Ilf3, supplied by Horizon Discovery, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ilf3/product/Horizon Discovery
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ilf3 - by Bioz Stars, 2020-08
    93/100 stars
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    ILF3 is phosphorylated in response to angiogenic stimuli in hCAECs. A ) Representative Western blot of ILF3 phosphorylation in response to VEGF stimulation. B ) Densiometric analysis of ILF3 phosphorylation in response to VEGF stimulation. Phosphorylation is normalized to total ILF3 protein. C ) Representative Western blot of ILF3 phosphorylation in response to IL-19 stimulation. D ) Densiometric analysis of ILF3 phosphorylation in response to IL-19 stimulation. hCAEC lysates were separated on a 7.5% gradient phos-tag gel to measure ILF3 phosphorylation. Total ILF3 was normalized to HSC70, and phosphorylated-ILF3 was made relative to normalized total ILF3 using densitometry. * P

    Journal: The FASEB Journal

    Article Title: RNA stability protein ILF3 mediates cytokine-induced angiogenesis

    doi: 10.1096/fj.201801315R

    Figure Lengend Snippet: ILF3 is phosphorylated in response to angiogenic stimuli in hCAECs. A ) Representative Western blot of ILF3 phosphorylation in response to VEGF stimulation. B ) Densiometric analysis of ILF3 phosphorylation in response to VEGF stimulation. Phosphorylation is normalized to total ILF3 protein. C ) Representative Western blot of ILF3 phosphorylation in response to IL-19 stimulation. D ) Densiometric analysis of ILF3 phosphorylation in response to IL-19 stimulation. hCAEC lysates were separated on a 7.5% gradient phos-tag gel to measure ILF3 phosphorylation. Total ILF3 was normalized to HSC70, and phosphorylated-ILF3 was made relative to normalized total ILF3 using densitometry. * P

    Article Snippet: In HeLa cells, ILF3 is considered to be predominantly nuclear, but becomes phosphorylated and translocates to the cytoplasm at the G2 /M boundary of the cell cycle ( ).

    Techniques: Western Blot

    ILF3 interacts with and stabilizes proangiogenic mRNA transcripts. A ) RNA immunoprecipitation. Flag-tagged AdILF3 was overexpressed in hCAECs and immunoprecipitated using flag or control IgG beads. RNA bound to the protein bead complex was isolated, and qRT-PCR was performed on the indicated transcript. Interaction is significantly increased in response to VEGF stimulation. B ) ILF3 knockdown by siRNA significantly reduces angiogenic transcript mRNA stability compared with scrambled controls. hCAECs were treated with VEGF for 16 h, and then RNA was isolated at various time points following actinomycin D (Act D) exposure. Transcript stability was determined by qRT-PCR. C ) ILF3 overexpression significantly increases angiogenic transcript mRNA stability. After infection with AdILF3 or AdGFP, hCAECs were stimulated with VEGF for 16 h, and then RNA was isolated at various time points following actinomycin D exposure. * P

    Journal: The FASEB Journal

    Article Title: RNA stability protein ILF3 mediates cytokine-induced angiogenesis

    doi: 10.1096/fj.201801315R

    Figure Lengend Snippet: ILF3 interacts with and stabilizes proangiogenic mRNA transcripts. A ) RNA immunoprecipitation. Flag-tagged AdILF3 was overexpressed in hCAECs and immunoprecipitated using flag or control IgG beads. RNA bound to the protein bead complex was isolated, and qRT-PCR was performed on the indicated transcript. Interaction is significantly increased in response to VEGF stimulation. B ) ILF3 knockdown by siRNA significantly reduces angiogenic transcript mRNA stability compared with scrambled controls. hCAECs were treated with VEGF for 16 h, and then RNA was isolated at various time points following actinomycin D (Act D) exposure. Transcript stability was determined by qRT-PCR. C ) ILF3 overexpression significantly increases angiogenic transcript mRNA stability. After infection with AdILF3 or AdGFP, hCAECs were stimulated with VEGF for 16 h, and then RNA was isolated at various time points following actinomycin D exposure. * P

    Article Snippet: In HeLa cells, ILF3 is considered to be predominantly nuclear, but becomes phosphorylated and translocates to the cytoplasm at the G2 /M boundary of the cell cycle ( ).

    Techniques: Immunoprecipitation, Isolation, Quantitative RT-PCR, Over Expression, Infection

    ILF3 expression regulates hCAEC proliferation. A ) ILF3 knockdown by siRNA significantly reduces hCAEC proliferation compared with scrambled control. B ) ILF3 overexpression significantly increases hCAEC proliferation compared with AdGFP controls. Cells were seeded at 20,000 cells/well and counted at d 3 and 6. C ) ILF3 knockdown by siRNA significantly reduces hCAEC migration in response to scratch wounding compared with scrambled control. D ) ILF3 overexpression significantly increases hCAEC migration compared to AdGFP controls. Cells were seeded at 100,000 cells/well; a 2-mm scratch was made, and images were taken immediately after and 24 h after the scratch. Area (percentage of control) was quantified using ImageJ to quantitate migration. Original magnification, ×100 for all images. E ) ILF3 knockdown confirmed with Western blotting using anti-ILF3 antibody. F ) ILF3 overexpression confirmed with Western blotting using anti-ILF3 antibody. Scale bars, 100 μm. * P

    Journal: The FASEB Journal

    Article Title: RNA stability protein ILF3 mediates cytokine-induced angiogenesis

    doi: 10.1096/fj.201801315R

    Figure Lengend Snippet: ILF3 expression regulates hCAEC proliferation. A ) ILF3 knockdown by siRNA significantly reduces hCAEC proliferation compared with scrambled control. B ) ILF3 overexpression significantly increases hCAEC proliferation compared with AdGFP controls. Cells were seeded at 20,000 cells/well and counted at d 3 and 6. C ) ILF3 knockdown by siRNA significantly reduces hCAEC migration in response to scratch wounding compared with scrambled control. D ) ILF3 overexpression significantly increases hCAEC migration compared to AdGFP controls. Cells were seeded at 100,000 cells/well; a 2-mm scratch was made, and images were taken immediately after and 24 h after the scratch. Area (percentage of control) was quantified using ImageJ to quantitate migration. Original magnification, ×100 for all images. E ) ILF3 knockdown confirmed with Western blotting using anti-ILF3 antibody. F ) ILF3 overexpression confirmed with Western blotting using anti-ILF3 antibody. Scale bars, 100 μm. * P

    Article Snippet: In HeLa cells, ILF3 is considered to be predominantly nuclear, but becomes phosphorylated and translocates to the cytoplasm at the G2 /M boundary of the cell cycle ( ).

    Techniques: Expressing, Over Expression, Migration, Western Blot

    ILF3 expression regulates hCAEC cord-like structure formation. A ) Representative image ILF3 knockdown by siRNA significantly reduces hCAEC cord-like structures compared with scrambled control. B ) The number of closed tubes and branches was quantified from 3 representative images from each condition performed in triplicate. C ) Representative image showing ILF3 overexpression significantly increases hCAEC cord-like structures compared with AdGFP control. D ) The number of closed tubes and branches was quantified from 3 representative images from each condition performed in triplicate. Original magnification, ×100 for all images. Scale bars, 100 μm. * P

    Journal: The FASEB Journal

    Article Title: RNA stability protein ILF3 mediates cytokine-induced angiogenesis

    doi: 10.1096/fj.201801315R

    Figure Lengend Snippet: ILF3 expression regulates hCAEC cord-like structure formation. A ) Representative image ILF3 knockdown by siRNA significantly reduces hCAEC cord-like structures compared with scrambled control. B ) The number of closed tubes and branches was quantified from 3 representative images from each condition performed in triplicate. C ) Representative image showing ILF3 overexpression significantly increases hCAEC cord-like structures compared with AdGFP control. D ) The number of closed tubes and branches was quantified from 3 representative images from each condition performed in triplicate. Original magnification, ×100 for all images. Scale bars, 100 μm. * P

    Article Snippet: In HeLa cells, ILF3 is considered to be predominantly nuclear, but becomes phosphorylated and translocates to the cytoplasm at the G2 /M boundary of the cell cycle ( ).

    Techniques: Expressing, Over Expression

    ILF3 expression regulates hCAEC angiogenic cytokine expression. A ) ILF3 knockdown by siRNA significantly reduces angiogenic cytokine mRNA compared with scrambled control. B ) ILF3 knockdown significantly reduces angiogenic cytokine protein abundance compared with scrambled controls. C ) ILF3 overexpression significantly increases angiogenic cytokine mRNA compared with AdGFP controls. D ) ILF3 overexpression significantly increases angiogenic cytokine protein abundance compared with scrambled controls. Cells were treated with 20 ng/ml of VEGF for 16 h for mRNA quantitation or 24 h for protein abundance, and protein levels were determined by Western blotting. HSC70, heat shock cognate protein 70. * P

    Journal: The FASEB Journal

    Article Title: RNA stability protein ILF3 mediates cytokine-induced angiogenesis

    doi: 10.1096/fj.201801315R

    Figure Lengend Snippet: ILF3 expression regulates hCAEC angiogenic cytokine expression. A ) ILF3 knockdown by siRNA significantly reduces angiogenic cytokine mRNA compared with scrambled control. B ) ILF3 knockdown significantly reduces angiogenic cytokine protein abundance compared with scrambled controls. C ) ILF3 overexpression significantly increases angiogenic cytokine mRNA compared with AdGFP controls. D ) ILF3 overexpression significantly increases angiogenic cytokine protein abundance compared with scrambled controls. Cells were treated with 20 ng/ml of VEGF for 16 h for mRNA quantitation or 24 h for protein abundance, and protein levels were determined by Western blotting. HSC70, heat shock cognate protein 70. * P

    Article Snippet: In HeLa cells, ILF3 is considered to be predominantly nuclear, but becomes phosphorylated and translocates to the cytoplasm at the G2 /M boundary of the cell cycle ( ).

    Techniques: Expressing, Over Expression, Quantitation Assay, Western Blot

    ILF3 translocates from the nucleus to the cytoplasm of hCAECs in response to antigenic stimuli. A ) Representative immunocytochemical analysis of ILF3 immunolocalization in hCAECs unstimulated, or stimulated with 20 ng/ml of VEGF or 100 ng/ml of IL-19 for 16 h. All wells were stained with anti-ILF3 antibody (green), DAPI (blue), and merged, in addition to negative controls. Original magnification, ×600. Scale bar, 10 μm. B ) Cellular fractionation of hCAECs stimulated with VEGF for various time points. ILF3 localization was determined by Western blot. C ) Cellular fractionation of hCAECs stimulated with IL-19 for various time points. ILF3 localization was determined by Western blot. GAPDH was used to confirm the cytoplasmic fraction, and proliferating cell nuclear antigen was used to show the lack of nuclear contamination in the cytoplasmic fraction. * P

    Journal: The FASEB Journal

    Article Title: RNA stability protein ILF3 mediates cytokine-induced angiogenesis

    doi: 10.1096/fj.201801315R

    Figure Lengend Snippet: ILF3 translocates from the nucleus to the cytoplasm of hCAECs in response to antigenic stimuli. A ) Representative immunocytochemical analysis of ILF3 immunolocalization in hCAECs unstimulated, or stimulated with 20 ng/ml of VEGF or 100 ng/ml of IL-19 for 16 h. All wells were stained with anti-ILF3 antibody (green), DAPI (blue), and merged, in addition to negative controls. Original magnification, ×600. Scale bar, 10 μm. B ) Cellular fractionation of hCAECs stimulated with VEGF for various time points. ILF3 localization was determined by Western blot. C ) Cellular fractionation of hCAECs stimulated with IL-19 for various time points. ILF3 localization was determined by Western blot. GAPDH was used to confirm the cytoplasmic fraction, and proliferating cell nuclear antigen was used to show the lack of nuclear contamination in the cytoplasmic fraction. * P

    Article Snippet: In HeLa cells, ILF3 is considered to be predominantly nuclear, but becomes phosphorylated and translocates to the cytoplasm at the G2 /M boundary of the cell cycle ( ).

    Techniques: Staining, Cell Fractionation, Western Blot

    ILF3 is expressed in angiogenic tissue and in response to angiogenic stimuli. A ) CD31 + vessels of ischemic porcine cardiac tissue. Representative immunohistochemical analysis of CD31 and ILF3 abundance in tissue stained with anti-CD31 or anti-ILF3 antibodies is shown. All sections were also stained with rabbit IgG negative control antibody. Original magnification, ×600. Scale bar, 100 μm. B ) ILF3 mRNA expression is induced in hCAECs in response to angiogenic cytokine treatment quantitated by qRT-PCR. C ) ILF3 protein is induced in response to angiogenic cytokines analyzed by Western blotting. Cells were treated with 100 ng/ml of IL-19 or 20 ng/ml of VEGF for 2, 4, 8, 16, or 24 h. D ) Densiometric analysis of ILF3 protein expression in hCAECs in response to angiogenic cytokine stimulation. * P

    Journal: The FASEB Journal

    Article Title: RNA stability protein ILF3 mediates cytokine-induced angiogenesis

    doi: 10.1096/fj.201801315R

    Figure Lengend Snippet: ILF3 is expressed in angiogenic tissue and in response to angiogenic stimuli. A ) CD31 + vessels of ischemic porcine cardiac tissue. Representative immunohistochemical analysis of CD31 and ILF3 abundance in tissue stained with anti-CD31 or anti-ILF3 antibodies is shown. All sections were also stained with rabbit IgG negative control antibody. Original magnification, ×600. Scale bar, 100 μm. B ) ILF3 mRNA expression is induced in hCAECs in response to angiogenic cytokine treatment quantitated by qRT-PCR. C ) ILF3 protein is induced in response to angiogenic cytokines analyzed by Western blotting. Cells were treated with 100 ng/ml of IL-19 or 20 ng/ml of VEGF for 2, 4, 8, 16, or 24 h. D ) Densiometric analysis of ILF3 protein expression in hCAECs in response to angiogenic cytokine stimulation. * P

    Article Snippet: In HeLa cells, ILF3 is considered to be predominantly nuclear, but becomes phosphorylated and translocates to the cytoplasm at the G2 /M boundary of the cell cycle ( ).

    Techniques: Immunohistochemistry, Staining, Negative Control, Expressing, Quantitative RT-PCR, Western Blot