non optical ion torrent sequencer  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier
Bioz Manufacturer Symbol Thermo Fisher manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 97
    Name:
    Ion Proton System
    Description:
    The Ion Proton System is the first benchtop sequencing system capable of human scale genome exome or transcriptome sequencing in a few hours with DNA to variants called in a single day The system combines semiconductor sequencing technology with natural biochemistry to directly translate chemical information into digital data By leveraging the exponential improvements in the semiconductor industry known as Moore s Law the Ion Proton Sequencing System provides an unprecedented level of scalability and flexibility to support a broad range of high throughput sequencing applications ranging from human scale genome to exome to transcriptome sequencing The system s use of the simplest natural sequencing chemistry eliminates the need for expensive optics and complex sequencing chemistries resulting in a highly affordable sequencing system to own and operate Real time direct electrical detection of sequencing combined with the enormous amount of computing power in both the Ion Proton Sequencer and Ion Proton Torrent Server enables generation of high quality sequencing results from DNA library to variants in a single day Features of the Ion Proton System • Fastest high throughput next generation sequencing workflow with the fastest sequencing run times of 2 4 hours on the Ion PI Chip • Highest number of complete sequencing runs per week with a simple and automated workflow when used with the Ion OneTouch 2 System • Simple semiconductor sequencing workflows with robust and simple hardware that can be relied upon no cameras no optics and no lasers • Scalable high throughput sequencing capabilities enabling up to 200 base single reads and flexible library options e g fragment gDNA targeted exome RNA • Small benchtop footprint and mounting capability with optional rack two Ion Proton systems per rack to deliver the only benchtop genome center • Low cost platform and attractively priced semiconductor chips and reagents for a range of applications • Proven Ion semiconductor sequencing requires no complex optics and employs natural nucleotides to deliver highly accurate variant detection uniformity of coverage and sensitivity to detect low frequency variants • Range of fast and simple library solutions and kits with low input requirements for a variety of applications such as genomic sequencing exome sequencing sequencing sets of genes and RNA sequencing • Complete end to end solution from base calls to variants with the Proton Torrent Server and Torrent Suite Software v3 0 • Simple and integrated tools for tertiary data analysis with Ion Reporter Software for DNA variation analysis across single paired or trio samples With the availability of the groundbreaking Ion Proton System rapid high throughput sequencing is finally accessible by all laboratories Affordable Sequencing For Nearly Every LabPrevious sequencing technologies have relied upon optical systems as bridges between the chemical and digital forms of sequence information These systems have added tremendous costs to sequencers relegating them to only the largest laboratories By eliminating the need for the optical system the Ion Proton System provides high throughput sequencing that is simpler faster more cost effective and more scalable than any other technology available With a benchtop footprint low cost sequencing reagents and a simple touchscreen user interface the Ion Proton System brings high throughput next generation sequencing to nearly every lab Ion Power PostLight Sequencing Produces Bits From BasesThe sequencing technology underlying the Ion Proton System exploits a well characterized biochemical process When a nucleotide is incorporated into a strand of DNA by a polymerase a hydrogen ion H is released as a byproduct This hydrogen ion carries a charge that the Proton System s ion sensor essentially the world s smallest solid state pH meter can detect As the sequencer floods the chip with one nucleotide after another any nucleotide added to a DNA template will be detected as a voltage change and the Ion Proton System will call the base If a nucleotide is not a match for a particular template no voltage change will be detected and no base will be called for that template Massively Parallel Sequencing On Your BenchA principal component of the Ion Proton System is the sequencing chip The Ion PI Chip incorporates an extremely dense array of 165 million micro machined wells married to our proprietary ion sensor Each well contains a different DNA template allowing massively parallel sequencing With the Ion PI Chip 165 million wells the Ion Proton System enables a range of high throughput genomic applications from human scale exome sequencing to whole transcriptome sequencing The Right Tool To Get Results Easily Reliably and QuicklyBecause it detects nucleotide incorporation without the use of light the Ion Proton System uses the simplest sequencing chemistry possible natural nucleotides There is no need for expensive and error prone modified bases enzymatic cascades chemiluminescence or fluorescence Direct detection also means the incorporation of each nucleotide is recorded in seconds As a result you can do an entire sequencing run typically in a few hours Services and Support Service and support plans comprehensive plans to maximize instrument performance and ensure availability of critical systems Qualification services document and ensure instrument is installed operating and performing to manufacturer s specifications Training courses for experimental design data analysis troubleshooting applications and instrument operation Bioinformatics tailored support for NGS informatics execution software use data analysis and downstream applications DataSafe Solution storage and back up strategy design hardware and support for all lab data
    Catalog Number:
    4476610
    Price:
    None
    Category:
    Instruments and Equipment
    Applications:
    DNA Sequencing|Epigenetic Sequencing|Fragment Analysis|Genotyping & Genomic Profiling|Ion Torrent™ Next-Generation Sequencing|Ion Torrent™ Systems & Accessories|Mitochondrial Sequencing|RNA Sequencing|SNP Genotyping by Fragment-Analysis|Sequencing|Small RNA & miRNA Sequencing|Methylation Analysis by Sequencing|Whole Genome Sequencing|Whole Transcriptome Sequencing|SNP Genotyping|Gene Expression Analysis & Genotyping|ChIP Sequencing|De Novo Sequencing|Gene Expression Profiling by Sequencing
    Buy from Supplier


    Structured Review

    Thermo Fisher non optical ion torrent sequencer
    Scalability of <t>Ion</t> <t>Torrent-compatible</t> MeDIP-Seq protocol. (a) Global 5-methylcytosine (5mC) content measured as a percentage of the genome in DNA from WT and DKO cells determined by an ELISA assay. (b) Number of peaks identified by MACS v2.1.0 software 64 from MeDIP-Seq data in WT and DKO cells. (c) Sequence coverage of genome-wide CpGs for WT and DKO cells on the Ion Torrent Proton <t>sequencer</t> calculated using MEDIPS v1.14.0 software. 39 Pie charts illustrate the fraction of CpGs covered by the indicated reads according to their fold-coverage in relation to the total genomic CpG content from MeDIP-Seq libraries of the indicated sample sequenced on the Proton with the total number of <t>non-redundant</t> mapped reads in parentheses. (d) Sequence coverage of genome-wide CpGs for WT and DKO cells in MeDIP-Seq on the Ion Torrent PGM sequencer. Data displayed as in c . (e) Scaled chromosomal view of the 5mC MeDIP-Seq enrichment of methylation over the MX1 gene in WT and DKO cells sequenced on the 2 different Ion Torrent sequencers as indicated. MeDIP-Seq data are displayed as RPM (Reads Per Million mapped reads) below the RefSeq annotation of the gene (blue lines). Methylation enrichment is displayed in gray, with red vertical lines corresponding to areas containing CpGs. Chromosome position and gene orientation are displayed.
    The Ion Proton System is the first benchtop sequencing system capable of human scale genome exome or transcriptome sequencing in a few hours with DNA to variants called in a single day The system combines semiconductor sequencing technology with natural biochemistry to directly translate chemical information into digital data By leveraging the exponential improvements in the semiconductor industry known as Moore s Law the Ion Proton Sequencing System provides an unprecedented level of scalability and flexibility to support a broad range of high throughput sequencing applications ranging from human scale genome to exome to transcriptome sequencing The system s use of the simplest natural sequencing chemistry eliminates the need for expensive optics and complex sequencing chemistries resulting in a highly affordable sequencing system to own and operate Real time direct electrical detection of sequencing combined with the enormous amount of computing power in both the Ion Proton Sequencer and Ion Proton Torrent Server enables generation of high quality sequencing results from DNA library to variants in a single day Features of the Ion Proton System • Fastest high throughput next generation sequencing workflow with the fastest sequencing run times of 2 4 hours on the Ion PI Chip • Highest number of complete sequencing runs per week with a simple and automated workflow when used with the Ion OneTouch 2 System • Simple semiconductor sequencing workflows with robust and simple hardware that can be relied upon no cameras no optics and no lasers • Scalable high throughput sequencing capabilities enabling up to 200 base single reads and flexible library options e g fragment gDNA targeted exome RNA • Small benchtop footprint and mounting capability with optional rack two Ion Proton systems per rack to deliver the only benchtop genome center • Low cost platform and attractively priced semiconductor chips and reagents for a range of applications • Proven Ion semiconductor sequencing requires no complex optics and employs natural nucleotides to deliver highly accurate variant detection uniformity of coverage and sensitivity to detect low frequency variants • Range of fast and simple library solutions and kits with low input requirements for a variety of applications such as genomic sequencing exome sequencing sequencing sets of genes and RNA sequencing • Complete end to end solution from base calls to variants with the Proton Torrent Server and Torrent Suite Software v3 0 • Simple and integrated tools for tertiary data analysis with Ion Reporter Software for DNA variation analysis across single paired or trio samples With the availability of the groundbreaking Ion Proton System rapid high throughput sequencing is finally accessible by all laboratories Affordable Sequencing For Nearly Every LabPrevious sequencing technologies have relied upon optical systems as bridges between the chemical and digital forms of sequence information These systems have added tremendous costs to sequencers relegating them to only the largest laboratories By eliminating the need for the optical system the Ion Proton System provides high throughput sequencing that is simpler faster more cost effective and more scalable than any other technology available With a benchtop footprint low cost sequencing reagents and a simple touchscreen user interface the Ion Proton System brings high throughput next generation sequencing to nearly every lab Ion Power PostLight Sequencing Produces Bits From BasesThe sequencing technology underlying the Ion Proton System exploits a well characterized biochemical process When a nucleotide is incorporated into a strand of DNA by a polymerase a hydrogen ion H is released as a byproduct This hydrogen ion carries a charge that the Proton System s ion sensor essentially the world s smallest solid state pH meter can detect As the sequencer floods the chip with one nucleotide after another any nucleotide added to a DNA template will be detected as a voltage change and the Ion Proton System will call the base If a nucleotide is not a match for a particular template no voltage change will be detected and no base will be called for that template Massively Parallel Sequencing On Your BenchA principal component of the Ion Proton System is the sequencing chip The Ion PI Chip incorporates an extremely dense array of 165 million micro machined wells married to our proprietary ion sensor Each well contains a different DNA template allowing massively parallel sequencing With the Ion PI Chip 165 million wells the Ion Proton System enables a range of high throughput genomic applications from human scale exome sequencing to whole transcriptome sequencing The Right Tool To Get Results Easily Reliably and QuicklyBecause it detects nucleotide incorporation without the use of light the Ion Proton System uses the simplest sequencing chemistry possible natural nucleotides There is no need for expensive and error prone modified bases enzymatic cascades chemiluminescence or fluorescence Direct detection also means the incorporation of each nucleotide is recorded in seconds As a result you can do an entire sequencing run typically in a few hours Services and Support Service and support plans comprehensive plans to maximize instrument performance and ensure availability of critical systems Qualification services document and ensure instrument is installed operating and performing to manufacturer s specifications Training courses for experimental design data analysis troubleshooting applications and instrument operation Bioinformatics tailored support for NGS informatics execution software use data analysis and downstream applications DataSafe Solution storage and back up strategy design hardware and support for all lab data
    https://www.bioz.com/result/non optical ion torrent sequencer/product/Thermo Fisher
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    non optical ion torrent sequencer - by Bioz Stars, 2021-04
    97/100 stars

    Images

    1) Product Images from "Semiconductor-based sequencing of genome-wide DNA methylation states"

    Article Title: Semiconductor-based sequencing of genome-wide DNA methylation states

    Journal: Epigenetics

    doi: 10.1080/15592294.2014.1003747

    Scalability of Ion Torrent-compatible MeDIP-Seq protocol. (a) Global 5-methylcytosine (5mC) content measured as a percentage of the genome in DNA from WT and DKO cells determined by an ELISA assay. (b) Number of peaks identified by MACS v2.1.0 software 64 from MeDIP-Seq data in WT and DKO cells. (c) Sequence coverage of genome-wide CpGs for WT and DKO cells on the Ion Torrent Proton sequencer calculated using MEDIPS v1.14.0 software. 39 Pie charts illustrate the fraction of CpGs covered by the indicated reads according to their fold-coverage in relation to the total genomic CpG content from MeDIP-Seq libraries of the indicated sample sequenced on the Proton with the total number of non-redundant mapped reads in parentheses. (d) Sequence coverage of genome-wide CpGs for WT and DKO cells in MeDIP-Seq on the Ion Torrent PGM sequencer. Data displayed as in c . (e) Scaled chromosomal view of the 5mC MeDIP-Seq enrichment of methylation over the MX1 gene in WT and DKO cells sequenced on the 2 different Ion Torrent sequencers as indicated. MeDIP-Seq data are displayed as RPM (Reads Per Million mapped reads) below the RefSeq annotation of the gene (blue lines). Methylation enrichment is displayed in gray, with red vertical lines corresponding to areas containing CpGs. Chromosome position and gene orientation are displayed.
    Figure Legend Snippet: Scalability of Ion Torrent-compatible MeDIP-Seq protocol. (a) Global 5-methylcytosine (5mC) content measured as a percentage of the genome in DNA from WT and DKO cells determined by an ELISA assay. (b) Number of peaks identified by MACS v2.1.0 software 64 from MeDIP-Seq data in WT and DKO cells. (c) Sequence coverage of genome-wide CpGs for WT and DKO cells on the Ion Torrent Proton sequencer calculated using MEDIPS v1.14.0 software. 39 Pie charts illustrate the fraction of CpGs covered by the indicated reads according to their fold-coverage in relation to the total genomic CpG content from MeDIP-Seq libraries of the indicated sample sequenced on the Proton with the total number of non-redundant mapped reads in parentheses. (d) Sequence coverage of genome-wide CpGs for WT and DKO cells in MeDIP-Seq on the Ion Torrent PGM sequencer. Data displayed as in c . (e) Scaled chromosomal view of the 5mC MeDIP-Seq enrichment of methylation over the MX1 gene in WT and DKO cells sequenced on the 2 different Ion Torrent sequencers as indicated. MeDIP-Seq data are displayed as RPM (Reads Per Million mapped reads) below the RefSeq annotation of the gene (blue lines). Methylation enrichment is displayed in gray, with red vertical lines corresponding to areas containing CpGs. Chromosome position and gene orientation are displayed.

    Techniques Used: Methylated DNA Immunoprecipitation, Enzyme-linked Immunosorbent Assay, Magnetic Cell Separation, Software, Sequencing, Genome Wide, Methylation

    Related Articles

    Sequencing:

    Article Title: Limits and patterns of cytomegalovirus genomic diversity in humans
    Article Snippet: .. Pooled DNA was then prepared for sequencing on the Illumina HiSeq2000 as described previously ( ) or for sequencing on the ION proton sequencer (Life Technologies) using the manufacturer’s standard protocols and reagents. .. Samples were labeled with DNA barcodes and aggregated into libraries that generally included four to six samples.

    Article Title: An Angiotensinogen Gene Polymorphism (rs5050) Is Associated with the Risk of Coronary Artery Aneurysm in Southern Chinese Children with Kawasaki Disease
    Article Snippet: The following steps were used for the PCR: 95°C for 3 min; 15 cycles of 95°C for 20 s, 58°C for 90 s, and 72°C for 30 s; and 72°C for 1 min using a GeneAmp PCR System 9700 (Thermo Fisher Scientific). .. The PCR products were subjected to massive parallel sequencing using an Ion Proton system (Life Technologies, CA, USA). ..

    Article Title: Drosophila Nipped-B Mutants Model Cornelia de Lange Syndrome in Growth and Behavior
    Article Snippet: Ribosomal RNA depletion and sequencing library construction for each wing disc RNA isolate were performed using Eukaryotic RiboMinus and Ion Total RNA-seq v2 kits (Life Technologies) according to the manufacturer’s directions. .. Sequencing was performed on an Ion Torrent Proton with a mean read length of 135 nucleotides, and reads were aligned to the release 5 Drosophila genome (April 2006) using the TMAP aligner map4 algorithm. ..

    Article Title: Application of Genotyping-by-Sequencing on Semiconductor Sequencing Platforms: A Comparison of Genetic and Reference-Based Marker Ordering in Barley
    Article Snippet: .. Conclusions We developed a GBS protocol suitable for sequencing on the Life Technologies semiconductor sequencing platforms, Ion Torrent PGM and Ion Torrent Proton. .. No major changes to the GBS approach were needed, only modifications to the adapter sequences to enable annealing and sequencing with the Ion Torrent chemistry.

    Article Title: Identification of NCAN as a candidate gene for developmental dyslexia
    Article Snippet: .. Next generation sequencing One hundred ng of genomic DNA from the affected individual 3935 was used for exome sequencing using an AmpliSeq library on the Ion Proton platform (Life Technologies, Carlsbad, CA) at the Uppsala Genome Center (Science for Life Laboratory, Uppsala University, Uppsala, Sweden). .. Variants identified using the Ion Torrent Suite software (Life Technologies) were filtered to retain only non-synonymous-, splice site- or stop codons variants.

    Polymerase Chain Reaction:

    Article Title: An Angiotensinogen Gene Polymorphism (rs5050) Is Associated with the Risk of Coronary Artery Aneurysm in Southern Chinese Children with Kawasaki Disease
    Article Snippet: The following steps were used for the PCR: 95°C for 3 min; 15 cycles of 95°C for 20 s, 58°C for 90 s, and 72°C for 30 s; and 72°C for 1 min using a GeneAmp PCR System 9700 (Thermo Fisher Scientific). .. The PCR products were subjected to massive parallel sequencing using an Ion Proton system (Life Technologies, CA, USA). ..

    RNA Sequencing Assay:

    Article Title: Comprehensive evaluation of AmpliSeq transcriptome, a novel targeted whole transcriptome RNA sequencing methodology for global gene expression analysis
    Article Snippet: .. The stimulated as well as unstimulated RNA samples from each cell line were prepared and sequenced using both the AmpliSeq and Proton RNA-seq on the Ion Torrent Proton. ..

    Chromatin Immunoprecipitation:

    Article Title: Amplicon-based semiconductor sequencing of human exomes: performance evaluation and optimization strategies
    Article Snippet: Of the reported 27 sequencing runs: the first 15, including 24 samples, were performed using Ion PI Template OT2 200 v2 and Ion PI Sequencing 200 v2 kits (Life Technologies); 5 runs, including 10 samples, were performed with v3 of the same kits; 5 runs, including 9 samples, were performed using Ion PI Hi-Q OT2 200 kit and Ion PI Hi-Q Sequencing 200 kit. .. Templated Ion Sphere Particles (ISP) were then enriched for positive ISP using Ion OneTouch ES (Life Technologies) and sequenced on Ion Proton sequencer using Ion PI chip v2 or v3 (Life Technologies), as reported in Supplementary table 7. .. Basecalling and sequence alignment were performed for all samples using Ion Torrent Suite software v.4.4.0.6 and genetic variants were then identified using Torrent Suite Variant Caller pipeline v.4.4.0.6 (TVC) with the optimized parameters provided by the manufacturer for AmpliSeq Exome.

    Next-Generation Sequencing:

    Article Title: Identification of NCAN as a candidate gene for developmental dyslexia
    Article Snippet: .. Next generation sequencing One hundred ng of genomic DNA from the affected individual 3935 was used for exome sequencing using an AmpliSeq library on the Ion Proton platform (Life Technologies, Carlsbad, CA) at the Uppsala Genome Center (Science for Life Laboratory, Uppsala University, Uppsala, Sweden). .. Variants identified using the Ion Torrent Suite software (Life Technologies) were filtered to retain only non-synonymous-, splice site- or stop codons variants.

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 97
    Thermo Fisher non optical ion torrent sequencer
    Scalability of <t>Ion</t> <t>Torrent-compatible</t> MeDIP-Seq protocol. (a) Global 5-methylcytosine (5mC) content measured as a percentage of the genome in DNA from WT and DKO cells determined by an ELISA assay. (b) Number of peaks identified by MACS v2.1.0 software 64 from MeDIP-Seq data in WT and DKO cells. (c) Sequence coverage of genome-wide CpGs for WT and DKO cells on the Ion Torrent Proton <t>sequencer</t> calculated using MEDIPS v1.14.0 software. 39 Pie charts illustrate the fraction of CpGs covered by the indicated reads according to their fold-coverage in relation to the total genomic CpG content from MeDIP-Seq libraries of the indicated sample sequenced on the Proton with the total number of <t>non-redundant</t> mapped reads in parentheses. (d) Sequence coverage of genome-wide CpGs for WT and DKO cells in MeDIP-Seq on the Ion Torrent PGM sequencer. Data displayed as in c . (e) Scaled chromosomal view of the 5mC MeDIP-Seq enrichment of methylation over the MX1 gene in WT and DKO cells sequenced on the 2 different Ion Torrent sequencers as indicated. MeDIP-Seq data are displayed as RPM (Reads Per Million mapped reads) below the RefSeq annotation of the gene (blue lines). Methylation enrichment is displayed in gray, with red vertical lines corresponding to areas containing CpGs. Chromosome position and gene orientation are displayed.
    Non Optical Ion Torrent Sequencer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/non optical ion torrent sequencer/product/Thermo Fisher
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    non optical ion torrent sequencer - by Bioz Stars, 2021-04
    97/100 stars
      Buy from Supplier

    97
    Thermo Fisher non optical ion torrent sequencing
    Scalability of <t>Ion</t> <t>Torrent-compatible</t> MeDIP-Seq protocol. (a) Global 5-methylcytosine (5mC) content measured as a percentage of the genome in DNA from WT and DKO cells determined by an ELISA assay. (b) Number of peaks identified by MACS v2.1.0 software 64 from MeDIP-Seq data in WT and DKO cells. (c) Sequence coverage of genome-wide CpGs for WT and DKO cells on the Ion Torrent Proton <t>sequencer</t> calculated using MEDIPS v1.14.0 software. 39 Pie charts illustrate the fraction of CpGs covered by the indicated reads according to their fold-coverage in relation to the total genomic CpG content from MeDIP-Seq libraries of the indicated sample sequenced on the Proton with the total number of <t>non-redundant</t> mapped reads in parentheses. (d) Sequence coverage of genome-wide CpGs for WT and DKO cells in MeDIP-Seq on the Ion Torrent PGM sequencer. Data displayed as in c . (e) Scaled chromosomal view of the 5mC MeDIP-Seq enrichment of methylation over the MX1 gene in WT and DKO cells sequenced on the 2 different Ion Torrent sequencers as indicated. MeDIP-Seq data are displayed as RPM (Reads Per Million mapped reads) below the RefSeq annotation of the gene (blue lines). Methylation enrichment is displayed in gray, with red vertical lines corresponding to areas containing CpGs. Chromosome position and gene orientation are displayed.
    Non Optical Ion Torrent Sequencing, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/non optical ion torrent sequencing/product/Thermo Fisher
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    non optical ion torrent sequencing - by Bioz Stars, 2021-04
    97/100 stars
      Buy from Supplier

    99
    Thermo Fisher non optical dna sequencing technology
    Scalability of <t>Ion</t> <t>Torrent-compatible</t> MeDIP-Seq protocol. (a) Global 5-methylcytosine (5mC) content measured as a percentage of the genome in DNA from WT and DKO cells determined by an ELISA assay. (b) Number of peaks identified by MACS v2.1.0 software 64 from MeDIP-Seq data in WT and DKO cells. (c) Sequence coverage of genome-wide CpGs for WT and DKO cells on the Ion Torrent Proton <t>sequencer</t> calculated using MEDIPS v1.14.0 software. 39 Pie charts illustrate the fraction of CpGs covered by the indicated reads according to their fold-coverage in relation to the total genomic CpG content from MeDIP-Seq libraries of the indicated sample sequenced on the Proton with the total number of <t>non-redundant</t> mapped reads in parentheses. (d) Sequence coverage of genome-wide CpGs for WT and DKO cells in MeDIP-Seq on the Ion Torrent PGM sequencer. Data displayed as in c . (e) Scaled chromosomal view of the 5mC MeDIP-Seq enrichment of methylation over the MX1 gene in WT and DKO cells sequenced on the 2 different Ion Torrent sequencers as indicated. MeDIP-Seq data are displayed as RPM (Reads Per Million mapped reads) below the RefSeq annotation of the gene (blue lines). Methylation enrichment is displayed in gray, with red vertical lines corresponding to areas containing CpGs. Chromosome position and gene orientation are displayed.
    Non Optical Dna Sequencing Technology, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/non optical dna sequencing technology/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    non optical dna sequencing technology - by Bioz Stars, 2021-04
    99/100 stars
      Buy from Supplier

    Image Search Results


    Scalability of Ion Torrent-compatible MeDIP-Seq protocol. (a) Global 5-methylcytosine (5mC) content measured as a percentage of the genome in DNA from WT and DKO cells determined by an ELISA assay. (b) Number of peaks identified by MACS v2.1.0 software 64 from MeDIP-Seq data in WT and DKO cells. (c) Sequence coverage of genome-wide CpGs for WT and DKO cells on the Ion Torrent Proton sequencer calculated using MEDIPS v1.14.0 software. 39 Pie charts illustrate the fraction of CpGs covered by the indicated reads according to their fold-coverage in relation to the total genomic CpG content from MeDIP-Seq libraries of the indicated sample sequenced on the Proton with the total number of non-redundant mapped reads in parentheses. (d) Sequence coverage of genome-wide CpGs for WT and DKO cells in MeDIP-Seq on the Ion Torrent PGM sequencer. Data displayed as in c . (e) Scaled chromosomal view of the 5mC MeDIP-Seq enrichment of methylation over the MX1 gene in WT and DKO cells sequenced on the 2 different Ion Torrent sequencers as indicated. MeDIP-Seq data are displayed as RPM (Reads Per Million mapped reads) below the RefSeq annotation of the gene (blue lines). Methylation enrichment is displayed in gray, with red vertical lines corresponding to areas containing CpGs. Chromosome position and gene orientation are displayed.

    Journal: Epigenetics

    Article Title: Semiconductor-based sequencing of genome-wide DNA methylation states

    doi: 10.1080/15592294.2014.1003747

    Figure Lengend Snippet: Scalability of Ion Torrent-compatible MeDIP-Seq protocol. (a) Global 5-methylcytosine (5mC) content measured as a percentage of the genome in DNA from WT and DKO cells determined by an ELISA assay. (b) Number of peaks identified by MACS v2.1.0 software 64 from MeDIP-Seq data in WT and DKO cells. (c) Sequence coverage of genome-wide CpGs for WT and DKO cells on the Ion Torrent Proton sequencer calculated using MEDIPS v1.14.0 software. 39 Pie charts illustrate the fraction of CpGs covered by the indicated reads according to their fold-coverage in relation to the total genomic CpG content from MeDIP-Seq libraries of the indicated sample sequenced on the Proton with the total number of non-redundant mapped reads in parentheses. (d) Sequence coverage of genome-wide CpGs for WT and DKO cells in MeDIP-Seq on the Ion Torrent PGM sequencer. Data displayed as in c . (e) Scaled chromosomal view of the 5mC MeDIP-Seq enrichment of methylation over the MX1 gene in WT and DKO cells sequenced on the 2 different Ion Torrent sequencers as indicated. MeDIP-Seq data are displayed as RPM (Reads Per Million mapped reads) below the RefSeq annotation of the gene (blue lines). Methylation enrichment is displayed in gray, with red vertical lines corresponding to areas containing CpGs. Chromosome position and gene orientation are displayed.

    Article Snippet: We therefore sought to determine how MeDIP-Seq data generated on a non-optical Ion Torrent sequencer (Ion Proton, Life Technologies) compares to that generated on an optical-based sequencing platform (GAIIx, Illumina).

    Techniques: Methylated DNA Immunoprecipitation, Enzyme-linked Immunosorbent Assay, Magnetic Cell Separation, Software, Sequencing, Genome Wide, Methylation