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Image Search Results


Figure 2. Rab2a interacts with the Noc2-Rab27a binary complex

Journal: Journal of cell science

Article Title: Rab2a and Rab27a cooperatively regulate the transition from granule maturation to exocytosis through the dual effector Noc2.

doi: 10.1242/jcs.195479

Figure Lengend Snippet: Figure 2. Rab2a interacts with the Noc2-Rab27a binary complex

Article Snippet: The following commercially purchased antibodies were also used: rabbit polyclonal antibodies toward FLAG (F7425, Sigma-Aldrich), hemagglutinin (HA; 561, MBL), green fluorescent protein (GFP; 598, MBL), Rab27a/b (18975, IBL), Noc2 (15297-1-AP, Proteintech), Rab2a (15420-1-AP, Proteintech), ICA69 (ab81500, Abcam), and PICK1 (ab3420, Abcam); and mouse monoclonal antibodies toward Rab3 (610379, BD Biosciences), EEA1 (610457, BD Biosciences), TGN38 (610849, BD Biosciences), PDI (MA3-018, Affinity BioReagents), α-tubulin (T5168, Sigma-Aldrich), and proinsulin (clone 3A1; ab8301, Abcam).

Techniques:

Figure 3. The N-terminal region of Noc2 is required for binding to Rab2a

Journal: Journal of cell science

Article Title: Rab2a and Rab27a cooperatively regulate the transition from granule maturation to exocytosis through the dual effector Noc2.

doi: 10.1242/jcs.195479

Figure Lengend Snippet: Figure 3. The N-terminal region of Noc2 is required for binding to Rab2a

Article Snippet: The following commercially purchased antibodies were also used: rabbit polyclonal antibodies toward FLAG (F7425, Sigma-Aldrich), hemagglutinin (HA; 561, MBL), green fluorescent protein (GFP; 598, MBL), Rab27a/b (18975, IBL), Noc2 (15297-1-AP, Proteintech), Rab2a (15420-1-AP, Proteintech), ICA69 (ab81500, Abcam), and PICK1 (ab3420, Abcam); and mouse monoclonal antibodies toward Rab3 (610379, BD Biosciences), EEA1 (610457, BD Biosciences), TGN38 (610849, BD Biosciences), PDI (MA3-018, Affinity BioReagents), α-tubulin (T5168, Sigma-Aldrich), and proinsulin (clone 3A1; ab8301, Abcam).

Techniques: Binding Assay

Figure 6. Effects of overexpression of Noc2 and its mutants on insulin secretion

Journal: Journal of cell science

Article Title: Rab2a and Rab27a cooperatively regulate the transition from granule maturation to exocytosis through the dual effector Noc2.

doi: 10.1242/jcs.195479

Figure Lengend Snippet: Figure 6. Effects of overexpression of Noc2 and its mutants on insulin secretion

Article Snippet: The following commercially purchased antibodies were also used: rabbit polyclonal antibodies toward FLAG (F7425, Sigma-Aldrich), hemagglutinin (HA; 561, MBL), green fluorescent protein (GFP; 598, MBL), Rab27a/b (18975, IBL), Noc2 (15297-1-AP, Proteintech), Rab2a (15420-1-AP, Proteintech), ICA69 (ab81500, Abcam), and PICK1 (ab3420, Abcam); and mouse monoclonal antibodies toward Rab3 (610379, BD Biosciences), EEA1 (610457, BD Biosciences), TGN38 (610849, BD Biosciences), PDI (MA3-018, Affinity BioReagents), α-tubulin (T5168, Sigma-Aldrich), and proinsulin (clone 3A1; ab8301, Abcam).

Techniques: Over Expression

Table 1

Journal:

Article Title: Immunohistochemical expression of rabphilin -3A-like (Noc2) in normal and tumor tissues of human endocrine pancreas

doi: 10.1080/10520290902738878

Figure Lengend Snippet: Table 1

Article Snippet: Polyclonal antisera were raised by immunizing rabbits with Noc2 specific peptide 1 (TPGRAD DPHFRPLPC) and peptide 2 (DRLPSTGVRDRK GDC) (GenScript Corp., Piscataway, NJ).

Techniques: