Structured Review

Millipore no synthase
Group mean ± s.e.m. change in cutaneous vascular conductance (Δ%CVC max ) as a percentage of maximal vasodilatation between the control site and the <t>NO-synthase-inhibited</t> site across the rise in body core temperature (Δ T or ,°C)
No Synthase, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Images

1) Product Images from "Up-regulation of arginase activity contributes to attenuated reflex cutaneous vasodilatation in hypertensive humans"

Article Title: Up-regulation of arginase activity contributes to attenuated reflex cutaneous vasodilatation in hypertensive humans

Journal:

doi: 10.1113/jphysiol.2007.128959

Group mean ± s.e.m. change in cutaneous vascular conductance (Δ%CVC max ) as a percentage of maximal vasodilatation between the control site and the NO-synthase-inhibited site across the rise in body core temperature (Δ T or ,°C)
Figure Legend Snippet: Group mean ± s.e.m. change in cutaneous vascular conductance (Δ%CVC max ) as a percentage of maximal vasodilatation between the control site and the NO-synthase-inhibited site across the rise in body core temperature (Δ T or ,°C)

Techniques Used:

2) Product Images from "Up-regulation of arginase activity contributes to attenuated reflex cutaneous vasodilatation in hypertensive humans"

Article Title: Up-regulation of arginase activity contributes to attenuated reflex cutaneous vasodilatation in hypertensive humans

Journal:

doi: 10.1113/jphysiol.2007.128959

Group mean ± s.e.m. change in cutaneous vascular conductance (Δ%CVC max ) as a percentage of maximal vasodilatation between the control site and the NO-synthase-inhibited site across the rise in body core temperature (Δ T or ,°C)
Figure Legend Snippet: Group mean ± s.e.m. change in cutaneous vascular conductance (Δ%CVC max ) as a percentage of maximal vasodilatation between the control site and the NO-synthase-inhibited site across the rise in body core temperature (Δ T or ,°C)

Techniques Used:

3) Product Images from "Up-regulation of arginase activity contributes to attenuated reflex cutaneous vasodilatation in hypertensive humans"

Article Title: Up-regulation of arginase activity contributes to attenuated reflex cutaneous vasodilatation in hypertensive humans

Journal:

doi: 10.1113/jphysiol.2007.128959

Group mean ± s.e.m. change in cutaneous vascular conductance (Δ%CVC max ) as a percentage of maximal vasodilatation between the control site and the NO-synthase-inhibited site across the rise in body core temperature (Δ T or ,°C)
Figure Legend Snippet: Group mean ± s.e.m. change in cutaneous vascular conductance (Δ%CVC max ) as a percentage of maximal vasodilatation between the control site and the NO-synthase-inhibited site across the rise in body core temperature (Δ T or ,°C)

Techniques Used:

Related Articles

Western Blot:

Article Title: Anomalous AMPK-regulated angiotensin AT1R expression and SIRT1-mediated mitochondrial biogenesis at RVLM in hypertension programming of offspring to maternal high fructose exposure
Article Snippet: Solubilized proteins were centrifuged at 20,000×g at 4 °C for 15 min, supernatant was collected and total protein was quantified by the Bradford assay with a protein assay kit (Bio-Rad, Hercules, CA). .. Western blot analysis Total protein extracted from RVLM samples was used to determine the expression levels of AT1 R, gp91phox , p67phox or p47phox subunits of NADPH oxidase, isoforms of superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase, nitric oxide synthase (NOS) isoforms, phosphorylated and total AMPK (p-AMPK and t-AMPK), sirtuin 1 (SIRT1), SIRT3, peroxisome proliferator-activated receptor gamma co-activator α (PGC-1α), mitochondrial transcription factor A (TFAM) or leptin by Western blot analysis. .. Protein samples were subject to 8–12% SDS-polyacrylamide gel electrophoresis and then transferred onto polyvinylidene difluoride transfer membranes (Immobilon-P membrane; Millipore, Bedford, MA) for 1.5 h at 4 °C, using a Bio-Rad miniprotein-III wet transfer unit (Bio-Rad).

Expressing:

Article Title: Anomalous AMPK-regulated angiotensin AT1R expression and SIRT1-mediated mitochondrial biogenesis at RVLM in hypertension programming of offspring to maternal high fructose exposure
Article Snippet: Solubilized proteins were centrifuged at 20,000×g at 4 °C for 15 min, supernatant was collected and total protein was quantified by the Bradford assay with a protein assay kit (Bio-Rad, Hercules, CA). .. Western blot analysis Total protein extracted from RVLM samples was used to determine the expression levels of AT1 R, gp91phox , p67phox or p47phox subunits of NADPH oxidase, isoforms of superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase, nitric oxide synthase (NOS) isoforms, phosphorylated and total AMPK (p-AMPK and t-AMPK), sirtuin 1 (SIRT1), SIRT3, peroxisome proliferator-activated receptor gamma co-activator α (PGC-1α), mitochondrial transcription factor A (TFAM) or leptin by Western blot analysis. .. Protein samples were subject to 8–12% SDS-polyacrylamide gel electrophoresis and then transferred onto polyvinylidene difluoride transfer membranes (Immobilon-P membrane; Millipore, Bedford, MA) for 1.5 h at 4 °C, using a Bio-Rad miniprotein-III wet transfer unit (Bio-Rad).

Pyrolysis Gas Chromatography:

Article Title: Anomalous AMPK-regulated angiotensin AT1R expression and SIRT1-mediated mitochondrial biogenesis at RVLM in hypertension programming of offspring to maternal high fructose exposure
Article Snippet: Solubilized proteins were centrifuged at 20,000×g at 4 °C for 15 min, supernatant was collected and total protein was quantified by the Bradford assay with a protein assay kit (Bio-Rad, Hercules, CA). .. Western blot analysis Total protein extracted from RVLM samples was used to determine the expression levels of AT1 R, gp91phox , p67phox or p47phox subunits of NADPH oxidase, isoforms of superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase, nitric oxide synthase (NOS) isoforms, phosphorylated and total AMPK (p-AMPK and t-AMPK), sirtuin 1 (SIRT1), SIRT3, peroxisome proliferator-activated receptor gamma co-activator α (PGC-1α), mitochondrial transcription factor A (TFAM) or leptin by Western blot analysis. .. Protein samples were subject to 8–12% SDS-polyacrylamide gel electrophoresis and then transferred onto polyvinylidene difluoride transfer membranes (Immobilon-P membrane; Millipore, Bedford, MA) for 1.5 h at 4 °C, using a Bio-Rad miniprotein-III wet transfer unit (Bio-Rad).

Immunostaining:

Article Title: Immunochemical characterization of inhibitory mouse cortical neurons: Three chemically distinct classes of inhibitory cells
Article Snippet: .. We examined immunostaining against CCK using the mouse monoclonal CCK antibody (The Center for Ulcer Research and Education (CURE), University of California (Los Angeles, CA), 9303, 1:1000) and the NOS antibody (Chemicon, AB1552, 1:250) in mouse cortical sections, but found that the CCK+ or NOS+ cells were quite scarce in mouse cortex. ..

other:

Article Title: Up-regulation of arginase activity contributes to attenuated reflex cutaneous vasodilatation in hypertensive humans
Article Snippet: Following this period, microdialysis sites were randomly assigned to receive: (1) 10.0 m m N G. -nitro- l -arginine ( l -NAME) to inhibit NO production by NO-synthase ( ; ; ); (2) a combination of 5.0 m m ( S )-(2-boronoethyl)- l -cysteine-HCl (BEC) and 5.0 m m N ω -hydroxy-nor- l -arginine (nor-NOHA) to inhibit arginase ( ) (Calbiochem, San Diego, CA, USA); (3) 10.0 m m l -arginine (Sigma) to supplement the substrate for NO-synthase and arginase ( ); or (4) 5.0 m m BEC + 5.0 m m nor-NOHA + 10.0 m m l -arginine to inhibit arginase and supplement the substrate for NO-synthase and arginase.

Incubation:

Article Title: Detection and characterisation of NAD(P)H-diaphorase activity in Dictyostelium discoideum cells (Protozoa)
Article Snippet: Non-specific binding sites were blocked with a blocking buffer containing Tris-buffered saline (pH 7.4) and 0.1% Tween-20 with 3% non-fat milk powder for 45 min at 25°C. .. The blot was incubated with a blocking buffer containing the anti-NOS universal (Sigma, N217), or brain (Sigma, N7155), or endothelial (Sigma, N3893) antibody produced in rabbit, diluted 1:500, overnight at 4°C. .. After washing, the blot was incubated with an anti-rabbit immunoglobulin secondary antibody conjugated with alkaline phosphatase, diluted 1:500 for 3 h at 25°C.

Blocking Assay:

Article Title: Detection and characterisation of NAD(P)H-diaphorase activity in Dictyostelium discoideum cells (Protozoa)
Article Snippet: Non-specific binding sites were blocked with a blocking buffer containing Tris-buffered saline (pH 7.4) and 0.1% Tween-20 with 3% non-fat milk powder for 45 min at 25°C. .. The blot was incubated with a blocking buffer containing the anti-NOS universal (Sigma, N217), or brain (Sigma, N7155), or endothelial (Sigma, N3893) antibody produced in rabbit, diluted 1:500, overnight at 4°C. .. After washing, the blot was incubated with an anti-rabbit immunoglobulin secondary antibody conjugated with alkaline phosphatase, diluted 1:500 for 3 h at 25°C.

Produced:

Article Title: Detection and characterisation of NAD(P)H-diaphorase activity in Dictyostelium discoideum cells (Protozoa)
Article Snippet: Non-specific binding sites were blocked with a blocking buffer containing Tris-buffered saline (pH 7.4) and 0.1% Tween-20 with 3% non-fat milk powder for 45 min at 25°C. .. The blot was incubated with a blocking buffer containing the anti-NOS universal (Sigma, N217), or brain (Sigma, N7155), or endothelial (Sigma, N3893) antibody produced in rabbit, diluted 1:500, overnight at 4°C. .. After washing, the blot was incubated with an anti-rabbit immunoglobulin secondary antibody conjugated with alkaline phosphatase, diluted 1:500 for 3 h at 25°C.

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  • 97
    Millipore glutamine synthase activity gs activity
    Nitration of glutamine <t>synthase</t> occurs following NMDA (2 nmol) induced thermal hyperalgesia ((a)–(c)). The time at which the NMDA mediated hyperalgesia was at its peak (40 minutes), immunoprecipitation analysis revealed that FeTM-4-PyP 5+ (2 nmol given 15 min before NMDA) reduced the NMDA mediated nitration of GS at the level of the spinal cord ((a)–(b)). Immunoprecipitation data are representative of at least 6 gels from 3 different animals performed on different days. Bar graph in (b) represents quantification by densitometric analysis. No difference for GS or β -actin expression was detected among the lanes in these conditions. † P
    Glutamine Synthase Activity Gs Activity, supplied by Millipore, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/glutamine synthase activity gs activity/product/Millipore
    Average 97 stars, based on 1 article reviews
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    94
    Millipore citrate synthase assay kit
    Rescue of aconitase defects in FRDA patient-derived lymphoblasts . ( A, B ) The effect of miniSINEUP-FXN expression on aconitase activity was measured on whole cells lysates. GM16214 cells expressing miniSINEUP-FXN showed restored activity of endogenous aconitase as compared to GM16215 positive control. Aconitase ( A ) and citrate <t>synthase</t> ( B ) activities are expressed as mU/mg ratio. Citrate synthase assay is used as an internal control. Columns represent mean ± S.E.M. ( n ≥ 4) of mU/mg values; ns, P > 0.05; * P
    Citrate Synthase Assay Kit, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Millipore rabbit anti phospho gsk 3α β
    LPA-induced <t>GSK-3</t> activation and tau phosphorylation in B103-LPA 1 cells. (A) GSK-3 kinase activity after addition of LPA (5 μM). Kinase activity at each time point was normalized with respect to the total amount of <t>GSK-3α/β</t> present
    Rabbit Anti Phospho Gsk 3α β, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti phospho gsk 3α β/product/Millipore
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    94
    Millipore gsk 3β
    Z-disk and M-band proteins are phosphorylated by CRT; role of <t>GSK-3β.</t>
    Gsk 3β, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gsk 3β/product/Millipore
    Average 94 stars, based on 1 article reviews
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    gsk 3β - by Bioz Stars, 2021-06
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    Image Search Results


    Nitration of glutamine synthase occurs following NMDA (2 nmol) induced thermal hyperalgesia ((a)–(c)). The time at which the NMDA mediated hyperalgesia was at its peak (40 minutes), immunoprecipitation analysis revealed that FeTM-4-PyP 5+ (2 nmol given 15 min before NMDA) reduced the NMDA mediated nitration of GS at the level of the spinal cord ((a)–(b)). Immunoprecipitation data are representative of at least 6 gels from 3 different animals performed on different days. Bar graph in (b) represents quantification by densitometric analysis. No difference for GS or β -actin expression was detected among the lanes in these conditions. † P

    Journal: Mediators of Inflammation

    Article Title: Posttranslational Nitration of Tyrosine Residues Modulates Glutamate Transmission and Contributes to N-Methyl-D-aspartate-Mediated Thermal Hyperalgesia

    doi: 10.1155/2013/950947

    Figure Lengend Snippet: Nitration of glutamine synthase occurs following NMDA (2 nmol) induced thermal hyperalgesia ((a)–(c)). The time at which the NMDA mediated hyperalgesia was at its peak (40 minutes), immunoprecipitation analysis revealed that FeTM-4-PyP 5+ (2 nmol given 15 min before NMDA) reduced the NMDA mediated nitration of GS at the level of the spinal cord ((a)–(b)). Immunoprecipitation data are representative of at least 6 gels from 3 different animals performed on different days. Bar graph in (b) represents quantification by densitometric analysis. No difference for GS or β -actin expression was detected among the lanes in these conditions. † P

    Article Snippet: Glutamine Synthase Activity GS activity was determined using a Glutamine/Glutamate Determination Kit (Sigma) following the manufacturer's protocol.

    Techniques: Nitration, Immunoprecipitation, Expressing

    Rescue of aconitase defects in FRDA patient-derived lymphoblasts . ( A, B ) The effect of miniSINEUP-FXN expression on aconitase activity was measured on whole cells lysates. GM16214 cells expressing miniSINEUP-FXN showed restored activity of endogenous aconitase as compared to GM16215 positive control. Aconitase ( A ) and citrate synthase ( B ) activities are expressed as mU/mg ratio. Citrate synthase assay is used as an internal control. Columns represent mean ± S.E.M. ( n ≥ 4) of mU/mg values; ns, P > 0.05; * P

    Journal: Nucleic Acids Research

    Article Title: SINEUP non-coding RNAs rescue defective frataxin expression and activity in a cellular model of Friedreich's Ataxia

    doi: 10.1093/nar/gkz798

    Figure Lengend Snippet: Rescue of aconitase defects in FRDA patient-derived lymphoblasts . ( A, B ) The effect of miniSINEUP-FXN expression on aconitase activity was measured on whole cells lysates. GM16214 cells expressing miniSINEUP-FXN showed restored activity of endogenous aconitase as compared to GM16215 positive control. Aconitase ( A ) and citrate synthase ( B ) activities are expressed as mU/mg ratio. Citrate synthase assay is used as an internal control. Columns represent mean ± S.E.M. ( n ≥ 4) of mU/mg values; ns, P > 0.05; * P

    Article Snippet: Spectrophotometric citrate synthase activities were assessed at 25°C with 10 μg of cell extracts using the Citrate Synthase Assay Kit (Sigma-Aldrich CS0720).

    Techniques: Derivative Assay, Expressing, Activity Assay, Positive Control

    LPA-induced GSK-3 activation and tau phosphorylation in B103-LPA 1 cells. (A) GSK-3 kinase activity after addition of LPA (5 μM). Kinase activity at each time point was normalized with respect to the total amount of GSK-3α/β present

    Journal:

    Article Title: GSK-3 Is Activated by the Tyrosine Kinase Pyk2 during LPA1-mediated Neurite Retraction

    doi: 10.1091/mbc.E05-07-0688

    Figure Lengend Snippet: LPA-induced GSK-3 activation and tau phosphorylation in B103-LPA 1 cells. (A) GSK-3 kinase activity after addition of LPA (5 μM). Kinase activity at each time point was normalized with respect to the total amount of GSK-3α/β present

    Article Snippet: The following antibodies were used: anti-GSK-3α/β monoclonal antibody (mAb) and rabbit anti-phospho-GSK-3α/β (pTyr279/216 ), Biosource (Camarillo, CA) and Sigma; rabbit phospho-GSK-3α/β (pSer21/9 ), Cell Signaling (Beverly, MA); anti-GSK-3β mAb, BD Transduction Laboratories (Lexington, KY).

    Techniques: Activation Assay, Activity Assay

    Z-disk and M-band proteins are phosphorylated by CRT; role of GSK-3β.

    Journal: The Journal of Clinical Investigation

    Article Title: Cardiac resynchronization sensitizes the sarcomere to calcium by reactivating GSK-3?

    doi: 10.1172/JCI69253

    Figure Lengend Snippet: Z-disk and M-band proteins are phosphorylated by CRT; role of GSK-3β.

    Article Snippet: Subsets of myocytes were exposed to either GSK-3β (10 μg/ml, 20 minutes; Sigma-Aldrich), PKA (0.125 units/ml, 20 minutes; Sigma-Aldrich), or Akt (10 μg/ml, 20 minutes; Sigma-Aldrich).

    Techniques:

    Z-disk and M-band proteins are phosphorylated by CRT; role of GSK-3β.

    Journal: The Journal of Clinical Investigation

    Article Title: Cardiac resynchronization sensitizes the sarcomere to calcium by reactivating GSK-3?

    doi: 10.1172/JCI69253

    Figure Lengend Snippet: Z-disk and M-band proteins are phosphorylated by CRT; role of GSK-3β.

    Article Snippet: Subsets of myocytes were exposed to either GSK-3β (10 μg/ml, 20 minutes; Sigma-Aldrich), PKA (0.125 units/ml, 20 minutes; Sigma-Aldrich), or Akt (10 μg/ml, 20 minutes; Sigma-Aldrich).

    Techniques:

    Increased phosphorylation at GSK-3β sites in CRT mediated the calcium-sensitizing effect.

    Journal: The Journal of Clinical Investigation

    Article Title: Cardiac resynchronization sensitizes the sarcomere to calcium by reactivating GSK-3?

    doi: 10.1172/JCI69253

    Figure Lengend Snippet: Increased phosphorylation at GSK-3β sites in CRT mediated the calcium-sensitizing effect.

    Article Snippet: Subsets of myocytes were exposed to either GSK-3β (10 μg/ml, 20 minutes; Sigma-Aldrich), PKA (0.125 units/ml, 20 minutes; Sigma-Aldrich), or Akt (10 μg/ml, 20 minutes; Sigma-Aldrich).

    Techniques: