no 114391 5g qiaquick gel extractio kit  (Qiagen)

 
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Name:
    QIAquick Gel Extraction Kit
    Description:
    For gel extraction cleanup of up to 10 μg DNA 70 bp to 10 kb from enzymatic reactions Kit contents Qiagen QIAquick Gel Extraction Kit 50 rxns 30 to 50L Elution Volume 10g Binding Capacity DNA Sample Tube Format Silica Technology Manual Processing 70 bp to 10 kb Fragment Fast and Convenient Procedure For Gel Extraction Cleanup of up to 10μg DNA 70 bp to 10 kb from Enzymatic Reactions Includes 50 QIAquick Spin Columns Buffers Collection Tubes 2mL Benefits Up to 95 recovery of ready to use DNA Fast and convenient procedure Cleanup of DNA up to 10 kb in three easy steps Gel loading dye for convenient sample analysis
    Catalog Number:
    28704
    Price:
    118
    Category:
    QIAquick Gel Extraction Kit
    Buy from Supplier


    Structured Review

    Qiagen no 114391 5g qiaquick gel extractio kit
    QIAquick Gel Extraction Kit
    For gel extraction cleanup of up to 10 μg DNA 70 bp to 10 kb from enzymatic reactions Kit contents Qiagen QIAquick Gel Extraction Kit 50 rxns 30 to 50L Elution Volume 10g Binding Capacity DNA Sample Tube Format Silica Technology Manual Processing 70 bp to 10 kb Fragment Fast and Convenient Procedure For Gel Extraction Cleanup of up to 10μg DNA 70 bp to 10 kb from Enzymatic Reactions Includes 50 QIAquick Spin Columns Buffers Collection Tubes 2mL Benefits Up to 95 recovery of ready to use DNA Fast and convenient procedure Cleanup of DNA up to 10 kb in three easy steps Gel loading dye for convenient sample analysis
    https://www.bioz.com/result/no 114391 5g qiaquick gel extractio kit/product/Qiagen
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    no 114391 5g qiaquick gel extractio kit - by Bioz Stars, 2020-08
    99/100 stars

    Images

    Related Articles

    Clone Assay:

    Article Title: Fluorescence-based methods for measuring target interference by CRISPR-Cas systems
    Article Snippet: .. T4 polynucleotide kinase (PNK), NotI, NcoI, T4 DNA ligase and accompanying buffers purchased from New England Biolabs 100 mM ATP 100 µM CRISPR target oligonucleotides (designed as in ) and pACYC-GFP Gel purification kit (e.g. Qiagen QIAquick Gel Extraction kit or Promega Wizard SV Gel and PCR Clean-Up System) One Shot TOP10 Competent Cells (Thermo-Fisher) or similar cloning E. coli strain Miniprep kit (Qiagen or Promega) ..

    Article Title: Small-scale transcriptomics reveals differences among gonadal stages in Asian seabass (Lates calcarifer)
    Article Snippet: .. The band of the expected size was cut from the gel, and then the amplified product was isolated using the QIAquick Gel Extraction Kit (Qiagen) and cloned into the pGEM-T Easy Vector (Promega). .. The validity of the sequence and the identification of the Asian seabass orthologous gene were completed by running the BLAST algorithm (blastn) against the NCBI RefSeq database (Additional file : Table S1).

    Amplification:

    Article Title: Time- and Behavioral State-Dependent Changes in Posterior Hypothalamic GABAA Receptors Contribute to the Regulation of Sleep
    Article Snippet: .. PCRs were calibrated using external cDNA standards obtained by amplification of the target cDNAs synthesized from total rat hypothalamic RNA, purification of the product with a gel extraction kit (QIA-quick; Qiagen), and then densitometric determination of cDNA concentration in the master standard solution. .. Calibration curves were then generated for each transcript as previously described .

    Article Title: Small-scale transcriptomics reveals differences among gonadal stages in Asian seabass (Lates calcarifer)
    Article Snippet: .. The band of the expected size was cut from the gel, and then the amplified product was isolated using the QIAquick Gel Extraction Kit (Qiagen) and cloned into the pGEM-T Easy Vector (Promega). .. The validity of the sequence and the identification of the Asian seabass orthologous gene were completed by running the BLAST algorithm (blastn) against the NCBI RefSeq database (Additional file : Table S1).

    Article Title: Distribution and phylogenetics of whiteflies and their endosymbiont relationships after the Mediterranean species invasion in Brazil
    Article Snippet: .. A representative number (121 out of 237) of PCR products amplified from mtCOI of the whiteflies were purified (QIAquick Gel Extraction Kit Qiagen) and sequenced (Macrogen, South Korea) in both directions using C1 -J-2195/TL2-N-3014 for B. tabaci and B. tuberculata or TvapF/Wfrev for T. vaporariorum . ..

    Article Title: High Prevalence of Hepatitis C Virus Genotype 1b Infection in a Small Town of Argentina. Phylogenetic and Bayesian Coalescent Analysis
    Article Snippet: .. The amplified DNAs were purified from agarose gels using a commercial kit (QIAquik Gel Extraction Kit protocol, QIAGEN) and the amplicons were sequenced in both senses using the internal PCR primers. ..

    Agarose Gel Electrophoresis:

    Article Title: An Ribonuclease T2 Family Protein Modulates Acinetobacter baumannii Abiotic Surface Colonization
    Article Snippet: .. PCR products were electrophoresed in a 1% UltraPure™ Agarose gel (Invitrogen), purified using a QIAquick® Gel Extraction Kit (Qiagen), ligated into pCR®II-TOPO® and transformed into E. coli One Shot® INVαF' cells, following the manufacturer's recommendations for Dual Promoter TA Cloning (Invitrogen). .. Following propagation, plasmid DNA was purified using QIAprep® Spin Miniprep Kits (Qiagen) and sequenced at the University of Nebraska Medical Center Sequencing Core Facility using vector specific primers (forward: 5′ -GTAAAACGACGGCCAG-3′ ; reverse: 5′ -CAGGAAACAGCTATGAC-3′ ).

    Synthesized:

    Article Title: Time- and Behavioral State-Dependent Changes in Posterior Hypothalamic GABAA Receptors Contribute to the Regulation of Sleep
    Article Snippet: .. PCRs were calibrated using external cDNA standards obtained by amplification of the target cDNAs synthesized from total rat hypothalamic RNA, purification of the product with a gel extraction kit (QIA-quick; Qiagen), and then densitometric determination of cDNA concentration in the master standard solution. .. Calibration curves were then generated for each transcript as previously described .

    Isolation:

    Article Title: Small-scale transcriptomics reveals differences among gonadal stages in Asian seabass (Lates calcarifer)
    Article Snippet: .. The band of the expected size was cut from the gel, and then the amplified product was isolated using the QIAquick Gel Extraction Kit (Qiagen) and cloned into the pGEM-T Easy Vector (Promega). .. The validity of the sequence and the identification of the Asian seabass orthologous gene were completed by running the BLAST algorithm (blastn) against the NCBI RefSeq database (Additional file : Table S1).

    TA Cloning:

    Article Title: An Ribonuclease T2 Family Protein Modulates Acinetobacter baumannii Abiotic Surface Colonization
    Article Snippet: .. PCR products were electrophoresed in a 1% UltraPure™ Agarose gel (Invitrogen), purified using a QIAquick® Gel Extraction Kit (Qiagen), ligated into pCR®II-TOPO® and transformed into E. coli One Shot® INVαF' cells, following the manufacturer's recommendations for Dual Promoter TA Cloning (Invitrogen). .. Following propagation, plasmid DNA was purified using QIAprep® Spin Miniprep Kits (Qiagen) and sequenced at the University of Nebraska Medical Center Sequencing Core Facility using vector specific primers (forward: 5′ -GTAAAACGACGGCCAG-3′ ; reverse: 5′ -CAGGAAACAGCTATGAC-3′ ).

    Purification:

    Article Title: Time- and Behavioral State-Dependent Changes in Posterior Hypothalamic GABAA Receptors Contribute to the Regulation of Sleep
    Article Snippet: .. PCRs were calibrated using external cDNA standards obtained by amplification of the target cDNAs synthesized from total rat hypothalamic RNA, purification of the product with a gel extraction kit (QIA-quick; Qiagen), and then densitometric determination of cDNA concentration in the master standard solution. .. Calibration curves were then generated for each transcript as previously described .

    Article Title: An Ribonuclease T2 Family Protein Modulates Acinetobacter baumannii Abiotic Surface Colonization
    Article Snippet: .. PCR products were electrophoresed in a 1% UltraPure™ Agarose gel (Invitrogen), purified using a QIAquick® Gel Extraction Kit (Qiagen), ligated into pCR®II-TOPO® and transformed into E. coli One Shot® INVαF' cells, following the manufacturer's recommendations for Dual Promoter TA Cloning (Invitrogen). .. Following propagation, plasmid DNA was purified using QIAprep® Spin Miniprep Kits (Qiagen) and sequenced at the University of Nebraska Medical Center Sequencing Core Facility using vector specific primers (forward: 5′ -GTAAAACGACGGCCAG-3′ ; reverse: 5′ -CAGGAAACAGCTATGAC-3′ ).

    Article Title: Distribution and phylogenetics of whiteflies and their endosymbiont relationships after the Mediterranean species invasion in Brazil
    Article Snippet: .. A representative number (121 out of 237) of PCR products amplified from mtCOI of the whiteflies were purified (QIAquick Gel Extraction Kit Qiagen) and sequenced (Macrogen, South Korea) in both directions using C1 -J-2195/TL2-N-3014 for B. tabaci and B. tuberculata or TvapF/Wfrev for T. vaporariorum . ..

    Article Title: High Prevalence of Hepatitis C Virus Genotype 1b Infection in a Small Town of Argentina. Phylogenetic and Bayesian Coalescent Analysis
    Article Snippet: .. The amplified DNAs were purified from agarose gels using a commercial kit (QIAquik Gel Extraction Kit protocol, QIAGEN) and the amplicons were sequenced in both senses using the internal PCR primers. ..

    Article Title: A toolbox of IgG subclass-switched recombinant monoclonal antibodies for enhanced multiplex immunolabeling of brain
    Article Snippet: .. The P1316 plasmid was also NotI/AscI digested and gel purified (Qiagen/QiaQuick Gel Extraction Cat# 28706). .. P1316 is a derivative of the pTT3 expression vector ( ) and consists of (5’ to 3’): a CMV promoter, the mouse V kappa leader sequence, a NotI restriction site, an insert consisting of VL /joining fragment/VH , and the mouse IgG1 CH sequence amplified from mouse genomic DNA, flanked by AscI and XbaI restriction sites ( ; ).

    Concentration Assay:

    Article Title: Time- and Behavioral State-Dependent Changes in Posterior Hypothalamic GABAA Receptors Contribute to the Regulation of Sleep
    Article Snippet: .. PCRs were calibrated using external cDNA standards obtained by amplification of the target cDNAs synthesized from total rat hypothalamic RNA, purification of the product with a gel extraction kit (QIA-quick; Qiagen), and then densitometric determination of cDNA concentration in the master standard solution. .. Calibration curves were then generated for each transcript as previously described .

    CRISPR:

    Article Title: Fluorescence-based methods for measuring target interference by CRISPR-Cas systems
    Article Snippet: .. T4 polynucleotide kinase (PNK), NotI, NcoI, T4 DNA ligase and accompanying buffers purchased from New England Biolabs 100 mM ATP 100 µM CRISPR target oligonucleotides (designed as in ) and pACYC-GFP Gel purification kit (e.g. Qiagen QIAquick Gel Extraction kit or Promega Wizard SV Gel and PCR Clean-Up System) One Shot TOP10 Competent Cells (Thermo-Fisher) or similar cloning E. coli strain Miniprep kit (Qiagen or Promega) ..

    Polymerase Chain Reaction:

    Article Title: An Ribonuclease T2 Family Protein Modulates Acinetobacter baumannii Abiotic Surface Colonization
    Article Snippet: .. PCR products were electrophoresed in a 1% UltraPure™ Agarose gel (Invitrogen), purified using a QIAquick® Gel Extraction Kit (Qiagen), ligated into pCR®II-TOPO® and transformed into E. coli One Shot® INVαF' cells, following the manufacturer's recommendations for Dual Promoter TA Cloning (Invitrogen). .. Following propagation, plasmid DNA was purified using QIAprep® Spin Miniprep Kits (Qiagen) and sequenced at the University of Nebraska Medical Center Sequencing Core Facility using vector specific primers (forward: 5′ -GTAAAACGACGGCCAG-3′ ; reverse: 5′ -CAGGAAACAGCTATGAC-3′ ).

    Article Title: Fluorescence-based methods for measuring target interference by CRISPR-Cas systems
    Article Snippet: .. T4 polynucleotide kinase (PNK), NotI, NcoI, T4 DNA ligase and accompanying buffers purchased from New England Biolabs 100 mM ATP 100 µM CRISPR target oligonucleotides (designed as in ) and pACYC-GFP Gel purification kit (e.g. Qiagen QIAquick Gel Extraction kit or Promega Wizard SV Gel and PCR Clean-Up System) One Shot TOP10 Competent Cells (Thermo-Fisher) or similar cloning E. coli strain Miniprep kit (Qiagen or Promega) ..

    Article Title: The Use of High-Throughput DNA Sequencing in the Investigation of Antigenic Variation: Application to Neisseria Species
    Article Snippet: .. The PCR products were then gel-purified with QIAquick Gel Extraction Kit (QIAGEN). .. The PCR product from the reference sample was then subjected to Sanger sequencing using the same primers that were used for amplification.

    Article Title: Distribution and phylogenetics of whiteflies and their endosymbiont relationships after the Mediterranean species invasion in Brazil
    Article Snippet: .. A representative number (121 out of 237) of PCR products amplified from mtCOI of the whiteflies were purified (QIAquick Gel Extraction Kit Qiagen) and sequenced (Macrogen, South Korea) in both directions using C1 -J-2195/TL2-N-3014 for B. tabaci and B. tuberculata or TvapF/Wfrev for T. vaporariorum . ..

    Article Title: High Prevalence of Hepatitis C Virus Genotype 1b Infection in a Small Town of Argentina. Phylogenetic and Bayesian Coalescent Analysis
    Article Snippet: .. The amplified DNAs were purified from agarose gels using a commercial kit (QIAquik Gel Extraction Kit protocol, QIAGEN) and the amplicons were sequenced in both senses using the internal PCR primers. ..

    Gel Extraction:

    Article Title: Time- and Behavioral State-Dependent Changes in Posterior Hypothalamic GABAA Receptors Contribute to the Regulation of Sleep
    Article Snippet: .. PCRs were calibrated using external cDNA standards obtained by amplification of the target cDNAs synthesized from total rat hypothalamic RNA, purification of the product with a gel extraction kit (QIA-quick; Qiagen), and then densitometric determination of cDNA concentration in the master standard solution. .. Calibration curves were then generated for each transcript as previously described .

    Article Title: An Ribonuclease T2 Family Protein Modulates Acinetobacter baumannii Abiotic Surface Colonization
    Article Snippet: .. PCR products were electrophoresed in a 1% UltraPure™ Agarose gel (Invitrogen), purified using a QIAquick® Gel Extraction Kit (Qiagen), ligated into pCR®II-TOPO® and transformed into E. coli One Shot® INVαF' cells, following the manufacturer's recommendations for Dual Promoter TA Cloning (Invitrogen). .. Following propagation, plasmid DNA was purified using QIAprep® Spin Miniprep Kits (Qiagen) and sequenced at the University of Nebraska Medical Center Sequencing Core Facility using vector specific primers (forward: 5′ -GTAAAACGACGGCCAG-3′ ; reverse: 5′ -CAGGAAACAGCTATGAC-3′ ).

    Article Title: Fluorescence-based methods for measuring target interference by CRISPR-Cas systems
    Article Snippet: .. T4 polynucleotide kinase (PNK), NotI, NcoI, T4 DNA ligase and accompanying buffers purchased from New England Biolabs 100 mM ATP 100 µM CRISPR target oligonucleotides (designed as in ) and pACYC-GFP Gel purification kit (e.g. Qiagen QIAquick Gel Extraction kit or Promega Wizard SV Gel and PCR Clean-Up System) One Shot TOP10 Competent Cells (Thermo-Fisher) or similar cloning E. coli strain Miniprep kit (Qiagen or Promega) ..

    Article Title: Small-scale transcriptomics reveals differences among gonadal stages in Asian seabass (Lates calcarifer)
    Article Snippet: .. The band of the expected size was cut from the gel, and then the amplified product was isolated using the QIAquick Gel Extraction Kit (Qiagen) and cloned into the pGEM-T Easy Vector (Promega). .. The validity of the sequence and the identification of the Asian seabass orthologous gene were completed by running the BLAST algorithm (blastn) against the NCBI RefSeq database (Additional file : Table S1).

    Article Title: The Use of High-Throughput DNA Sequencing in the Investigation of Antigenic Variation: Application to Neisseria Species
    Article Snippet: .. The PCR products were then gel-purified with QIAquick Gel Extraction Kit (QIAGEN). .. The PCR product from the reference sample was then subjected to Sanger sequencing using the same primers that were used for amplification.

    Article Title: Distribution and phylogenetics of whiteflies and their endosymbiont relationships after the Mediterranean species invasion in Brazil
    Article Snippet: .. A representative number (121 out of 237) of PCR products amplified from mtCOI of the whiteflies were purified (QIAquick Gel Extraction Kit Qiagen) and sequenced (Macrogen, South Korea) in both directions using C1 -J-2195/TL2-N-3014 for B. tabaci and B. tuberculata or TvapF/Wfrev for T. vaporariorum . ..

    Article Title: High Prevalence of Hepatitis C Virus Genotype 1b Infection in a Small Town of Argentina. Phylogenetic and Bayesian Coalescent Analysis
    Article Snippet: .. The amplified DNAs were purified from agarose gels using a commercial kit (QIAquik Gel Extraction Kit protocol, QIAGEN) and the amplicons were sequenced in both senses using the internal PCR primers. ..

    Article Title: A toolbox of IgG subclass-switched recombinant monoclonal antibodies for enhanced multiplex immunolabeling of brain
    Article Snippet: .. The P1316 plasmid was also NotI/AscI digested and gel purified (Qiagen/QiaQuick Gel Extraction Cat# 28706). .. P1316 is a derivative of the pTT3 expression vector ( ) and consists of (5’ to 3’): a CMV promoter, the mouse V kappa leader sequence, a NotI restriction site, an insert consisting of VL /joining fragment/VH , and the mouse IgG1 CH sequence amplified from mouse genomic DNA, flanked by AscI and XbaI restriction sites ( ; ).

    Transformation Assay:

    Article Title: An Ribonuclease T2 Family Protein Modulates Acinetobacter baumannii Abiotic Surface Colonization
    Article Snippet: .. PCR products were electrophoresed in a 1% UltraPure™ Agarose gel (Invitrogen), purified using a QIAquick® Gel Extraction Kit (Qiagen), ligated into pCR®II-TOPO® and transformed into E. coli One Shot® INVαF' cells, following the manufacturer's recommendations for Dual Promoter TA Cloning (Invitrogen). .. Following propagation, plasmid DNA was purified using QIAprep® Spin Miniprep Kits (Qiagen) and sequenced at the University of Nebraska Medical Center Sequencing Core Facility using vector specific primers (forward: 5′ -GTAAAACGACGGCCAG-3′ ; reverse: 5′ -CAGGAAACAGCTATGAC-3′ ).

    Plasmid Preparation:

    Article Title: Small-scale transcriptomics reveals differences among gonadal stages in Asian seabass (Lates calcarifer)
    Article Snippet: .. The band of the expected size was cut from the gel, and then the amplified product was isolated using the QIAquick Gel Extraction Kit (Qiagen) and cloned into the pGEM-T Easy Vector (Promega). .. The validity of the sequence and the identification of the Asian seabass orthologous gene were completed by running the BLAST algorithm (blastn) against the NCBI RefSeq database (Additional file : Table S1).

    Article Title: A toolbox of IgG subclass-switched recombinant monoclonal antibodies for enhanced multiplex immunolabeling of brain
    Article Snippet: .. The P1316 plasmid was also NotI/AscI digested and gel purified (Qiagen/QiaQuick Gel Extraction Cat# 28706). .. P1316 is a derivative of the pTT3 expression vector ( ) and consists of (5’ to 3’): a CMV promoter, the mouse V kappa leader sequence, a NotI restriction site, an insert consisting of VL /joining fragment/VH , and the mouse IgG1 CH sequence amplified from mouse genomic DNA, flanked by AscI and XbaI restriction sites ( ; ).

    Gel Purification:

    Article Title: Fluorescence-based methods for measuring target interference by CRISPR-Cas systems
    Article Snippet: .. T4 polynucleotide kinase (PNK), NotI, NcoI, T4 DNA ligase and accompanying buffers purchased from New England Biolabs 100 mM ATP 100 µM CRISPR target oligonucleotides (designed as in ) and pACYC-GFP Gel purification kit (e.g. Qiagen QIAquick Gel Extraction kit or Promega Wizard SV Gel and PCR Clean-Up System) One Shot TOP10 Competent Cells (Thermo-Fisher) or similar cloning E. coli strain Miniprep kit (Qiagen or Promega) ..

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Qiagen no 114391 5g qiaquick gel extractio kit
    No 114391 5g Qiaquick Gel Extractio Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/no 114391 5g qiaquick gel extractio kit/product/Qiagen
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    no 114391 5g qiaquick gel extractio kit - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    Image Search Results