nlaiv  (New England Biolabs)


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    New England Biolabs nlaiv
    Nlaiv, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    nlaiv  (New England Biolabs)


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    New England Biolabs nlaiv
    Nlaiv, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    nla iv  (New England Biolabs)


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    New England Biolabs nla iv
    Key to grass identification at Sourhope using trn L PCR-RFLP. Fragment sizes were determined using nucleotide sequence data of the trn L PCR product of each reference species. Restriction enzyme(s) are followed by the fragment sizes expected from digestion with the enzyme(s). GenBank accession numbers are provided in brackets following the species name.
    Nla Iv, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 94 stars, based on 1 article reviews
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    nla iv - by Bioz Stars, 2023-06
    94/100 stars

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    1) Product Images from "Identification of roots from grass swards using PCR-RFLP and FFLP of the plastid trn L (UAA) intron"

    Article Title: Identification of roots from grass swards using PCR-RFLP and FFLP of the plastid trn L (UAA) intron

    Journal: BMC Ecology

    doi: 10.1186/1472-6785-3-8

    Key to grass identification at Sourhope using trn L PCR-RFLP. Fragment sizes were determined using nucleotide sequence data of the trn L PCR product of each reference species. Restriction enzyme(s) are followed by the fragment sizes expected from digestion with the enzyme(s). GenBank accession numbers are provided in brackets following the species name.
    Figure Legend Snippet: Key to grass identification at Sourhope using trn L PCR-RFLP. Fragment sizes were determined using nucleotide sequence data of the trn L PCR product of each reference species. Restriction enzyme(s) are followed by the fragment sizes expected from digestion with the enzyme(s). GenBank accession numbers are provided in brackets following the species name.

    Techniques Used: Sequencing

    nla iv  (New England Biolabs)


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    New England Biolabs nla iv
    Key to grass identification at Sourhope using trn L PCR-RFLP. Fragment sizes were determined using nucleotide sequence data of the trn L PCR product of each reference species. Restriction enzyme(s) are followed by the fragment sizes expected from digestion with the enzyme(s). GenBank accession numbers are provided in brackets following the species name.
    Nla Iv, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nla iv/product/New England Biolabs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    nla iv - by Bioz Stars, 2023-06
    94/100 stars

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    1) Product Images from "Identification of roots from grass swards using PCR-RFLP and FFLP of the plastid trn L (UAA) intron"

    Article Title: Identification of roots from grass swards using PCR-RFLP and FFLP of the plastid trn L (UAA) intron

    Journal: BMC Ecology

    doi: 10.1186/1472-6785-3-8

    Key to grass identification at Sourhope using trn L PCR-RFLP. Fragment sizes were determined using nucleotide sequence data of the trn L PCR product of each reference species. Restriction enzyme(s) are followed by the fragment sizes expected from digestion with the enzyme(s). GenBank accession numbers are provided in brackets following the species name.
    Figure Legend Snippet: Key to grass identification at Sourhope using trn L PCR-RFLP. Fragment sizes were determined using nucleotide sequence data of the trn L PCR product of each reference species. Restriction enzyme(s) are followed by the fragment sizes expected from digestion with the enzyme(s). GenBank accession numbers are provided in brackets following the species name.

    Techniques Used: Sequencing

    nlaiv  (New England Biolabs)


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    New England Biolabs nlaiv
    a) Sequence data showing C/T heterozygosity at position -57; C = cytosine, G = guanine, Y = pyrimidine b) Digest <t>of</t> <t>PCR</t> products of regulatory region amplification with enzyme <t>NlaIV,</t> showing a restriction fragment length polymorphism in case B, due to the presence of allele -57T. NlaIV cleaves the PCR product in the presence of the C allele, but not the T allele. Lane one contains the hyperladder marker of molecular weight.
    Nlaiv, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    nlaiv - by Bioz Stars, 2023-06
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    1) Product Images from "Screening of the transcriptional regulatory regions of vascular endothelial growth factor receptor 2 (VEGFR2) in amyotrophic lateral sclerosis"

    Article Title: Screening of the transcriptional regulatory regions of vascular endothelial growth factor receptor 2 (VEGFR2) in amyotrophic lateral sclerosis

    Journal: BMC Medical Genetics

    doi: 10.1186/1471-2350-8-23

    a) Sequence data showing C/T heterozygosity at position -57; C = cytosine, G = guanine, Y = pyrimidine b) Digest of PCR products of regulatory region amplification with enzyme NlaIV, showing a restriction fragment length polymorphism in case B, due to the presence of allele -57T. NlaIV cleaves the PCR product in the presence of the C allele, but not the T allele. Lane one contains the hyperladder marker of molecular weight.
    Figure Legend Snippet: a) Sequence data showing C/T heterozygosity at position -57; C = cytosine, G = guanine, Y = pyrimidine b) Digest of PCR products of regulatory region amplification with enzyme NlaIV, showing a restriction fragment length polymorphism in case B, due to the presence of allele -57T. NlaIV cleaves the PCR product in the presence of the C allele, but not the T allele. Lane one contains the hyperladder marker of molecular weight.

    Techniques Used: Sequencing, Amplification, Marker, Molecular Weight

    nla iv  (New England Biolabs)


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    New England Biolabs nla iv
    Nla Iv, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    nla iv  (New England Biolabs)


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    New England Biolabs nla iv
    Transforming DNA plasmids are based on pilG (black) interrupted by an erythromycin resistance insert, ermC , (gray). DUS ( 5′- ATGCCGTCTGAA-3′ ) or reverse complimentary DUS ( 5′-TTCAGACGGCAT-3′ ) are inserted in three different positions A, B and C as marked above the bar. All numbers refer to the nucleotide position following the start codon (1) of pilG. The 137 nucleotide long Bam HI-fragment which is removed in pDV-b, pDV-c versions is shown in white with black stripes. B. <t>Nla</t> IV restriction profile <t>of</t> <t>rpoB</t> and location of selective SNP. The homologous 723 nt long PCR fragment of an internal part of the meningococcal rpoB gene used in transformation contains two Nla IV restriction sites on both sides of the selective SNP responsible for rifampicin resistance in the recipient.
    Nla Iv, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Restriction and Sequence Alterations Affect DNA Uptake Sequence-Dependent Transformation in Neisseria meningitidis"

    Article Title: Restriction and Sequence Alterations Affect DNA Uptake Sequence-Dependent Transformation in Neisseria meningitidis

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0039742

    Transforming DNA plasmids are based on pilG (black) interrupted by an erythromycin resistance insert, ermC , (gray). DUS ( 5′- ATGCCGTCTGAA-3′ ) or reverse complimentary DUS ( 5′-TTCAGACGGCAT-3′ ) are inserted in three different positions A, B and C as marked above the bar. All numbers refer to the nucleotide position following the start codon (1) of pilG. The 137 nucleotide long Bam HI-fragment which is removed in pDV-b, pDV-c versions is shown in white with black stripes. B. Nla IV restriction profile of rpoB and location of selective SNP. The homologous 723 nt long PCR fragment of an internal part of the meningococcal rpoB gene used in transformation contains two Nla IV restriction sites on both sides of the selective SNP responsible for rifampicin resistance in the recipient.
    Figure Legend Snippet: Transforming DNA plasmids are based on pilG (black) interrupted by an erythromycin resistance insert, ermC , (gray). DUS ( 5′- ATGCCGTCTGAA-3′ ) or reverse complimentary DUS ( 5′-TTCAGACGGCAT-3′ ) are inserted in three different positions A, B and C as marked above the bar. All numbers refer to the nucleotide position following the start codon (1) of pilG. The 137 nucleotide long Bam HI-fragment which is removed in pDV-b, pDV-c versions is shown in white with black stripes. B. Nla IV restriction profile of rpoB and location of selective SNP. The homologous 723 nt long PCR fragment of an internal part of the meningococcal rpoB gene used in transformation contains two Nla IV restriction sites on both sides of the selective SNP responsible for rifampicin resistance in the recipient.

    Techniques Used: Transformation Assay

    The Y axis shows the number of resistant (erythromycin) CFU/total 10 10 CFU on a log scale. Along the X-axis are the different DNA substrates (10 ng/ml) with altered numbers of Nla IV restriction sites shown. pDV4-a harbours two and three Nla IV restriction sites more than pDV4-b and pDV4-c, respectively. For further details of the restriction profiles and DUS locations in the transforming DNA plasmids please consult and . Statistically significant differences in transformation frequencies between the Nla IV null mutant and wildtype backgrounds are indicated above the columns, ***equals p≤0.001 in a two tailed paired student’s t-test.
    Figure Legend Snippet: The Y axis shows the number of resistant (erythromycin) CFU/total 10 10 CFU on a log scale. Along the X-axis are the different DNA substrates (10 ng/ml) with altered numbers of Nla IV restriction sites shown. pDV4-a harbours two and three Nla IV restriction sites more than pDV4-b and pDV4-c, respectively. For further details of the restriction profiles and DUS locations in the transforming DNA plasmids please consult and . Statistically significant differences in transformation frequencies between the Nla IV null mutant and wildtype backgrounds are indicated above the columns, ***equals p≤0.001 in a two tailed paired student’s t-test.

    Techniques Used: Transformation Assay, Mutagenesis, Two Tailed Test

    The Y axis shows the number of rifampicin-resistant CFU/total CFU 10 9 on a log scale. Along the X-axis are shown the two different DNA substrates (5 ng/ml), rpoB PCR fragment and rpoB PCR fragment pre-digested with Nla IV. For further details on the transforming DNA please consult and . The transformation frequencies in the wildtype and in the Nla IV null mutant backgrounds are not statistically significant from each other.
    Figure Legend Snippet: The Y axis shows the number of rifampicin-resistant CFU/total CFU 10 9 on a log scale. Along the X-axis are shown the two different DNA substrates (5 ng/ml), rpoB PCR fragment and rpoB PCR fragment pre-digested with Nla IV. For further details on the transforming DNA please consult and . The transformation frequencies in the wildtype and in the Nla IV null mutant backgrounds are not statistically significant from each other.

    Techniques Used: Transformation Assay, Mutagenesis

    The Y axis shows the number of erythromycin-resistant CFU/total CFU 10 8 on a log scale. Along the X-axis are shown the transforming DNA (1 µg/ml) that differ in Nla IV restriction profiles and DUS location. For further details on the DNA plasmid templates please consult and . Statistically significant values are indicated above the columns, **equals p≤0.05 and ***equals p≤0.001 in a two-tailed paired student’s t tests.
    Figure Legend Snippet: The Y axis shows the number of erythromycin-resistant CFU/total CFU 10 8 on a log scale. Along the X-axis are shown the transforming DNA (1 µg/ml) that differ in Nla IV restriction profiles and DUS location. For further details on the DNA plasmid templates please consult and . Statistically significant values are indicated above the columns, **equals p≤0.05 and ***equals p≤0.001 in a two-tailed paired student’s t tests.

    Techniques Used: Plasmid Preparation, Two Tailed Test

    Fold-differences and statistical significance values (paired two tailed student’s t tests) in comparing the performances of individual plasmids harbouring DUS in the C (pDV1) or A (pDV4) positions in transformation of the  Nla IV  mutant and wt backgrounds.
    Figure Legend Snippet: Fold-differences and statistical significance values (paired two tailed student’s t tests) in comparing the performances of individual plasmids harbouring DUS in the C (pDV1) or A (pDV4) positions in transformation of the Nla IV mutant and wt backgrounds.

    Techniques Used: Two Tailed Test, Transformation Assay, Mutagenesis

    Plasmids and bacterial strains.
    Figure Legend Snippet: Plasmids and bacterial strains.

    Techniques Used: Clone Assay, Plasmid Preparation, Isolation, Mutagenesis

    nla iv  (New England Biolabs)


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    New England Biolabs nla iv
    Nla Iv, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    nlaiv restriction enzyme  (New England Biolabs)


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    New England Biolabs nlaiv restriction enzyme
    Nlaiv Restriction Enzyme, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    nla iv  (New England Biolabs)


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    New England Biolabs nla iv

    Nla Iv, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Mapping and CRISPR homology-directed repair of a recessive white eye mutation in Plodia moths"

    Article Title: Mapping and CRISPR homology-directed repair of a recessive white eye mutation in Plodia moths

    Journal: iScience

    doi: 10.1016/j.isci.2022.103885


    Figure Legend Snippet:

    Techniques Used: Recombinant, Nucleic Acid Electrophoresis, Mutagenesis, Software, Cell Culture, Sequencing

    r0126s  (New England Biolabs)


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    New England Biolabs r0126s

    R0126s, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Mapping and CRISPR homology-directed repair of a recessive white eye mutation in Plodia moths"

    Article Title: Mapping and CRISPR homology-directed repair of a recessive white eye mutation in Plodia moths

    Journal: iScience

    doi: 10.1016/j.isci.2022.103885


    Figure Legend Snippet:

    Techniques Used: Recombinant, Nucleic Acid Electrophoresis, Mutagenesis, Software, Cell Culture, Sequencing

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    New England Biolabs nlaiv
    Nlaiv, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs nla iv
    Key to grass identification at Sourhope using trn L PCR-RFLP. Fragment sizes were determined using nucleotide sequence data of the trn L PCR product of each reference species. Restriction enzyme(s) are followed by the fragment sizes expected from digestion with the enzyme(s). GenBank accession numbers are provided in brackets following the species name.
    Nla Iv, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs nlaiv restriction enzyme
    Key to grass identification at Sourhope using trn L PCR-RFLP. Fragment sizes were determined using nucleotide sequence data of the trn L PCR product of each reference species. Restriction enzyme(s) are followed by the fragment sizes expected from digestion with the enzyme(s). GenBank accession numbers are provided in brackets following the species name.
    Nlaiv Restriction Enzyme, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs r0126s

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    Key to grass identification at Sourhope using trn L PCR-RFLP. Fragment sizes were determined using nucleotide sequence data of the trn L PCR product of each reference species. Restriction enzyme(s) are followed by the fragment sizes expected from digestion with the enzyme(s). GenBank accession numbers are provided in brackets following the species name.

    Journal: BMC Ecology

    Article Title: Identification of roots from grass swards using PCR-RFLP and FFLP of the plastid trn L (UAA) intron

    doi: 10.1186/1472-6785-3-8

    Figure Lengend Snippet: Key to grass identification at Sourhope using trn L PCR-RFLP. Fragment sizes were determined using nucleotide sequence data of the trn L PCR product of each reference species. Restriction enzyme(s) are followed by the fragment sizes expected from digestion with the enzyme(s). GenBank accession numbers are provided in brackets following the species name.

    Article Snippet: The double digest with Nla IV and Dde I was performed in Promega buffer B. Bfa I and Nla IV were obtained from New England Biolabs; Dde I, Hin fI, Hsp 92II and Xmn I from Promega.

    Techniques: Sequencing

    Journal: iScience

    Article Title: Mapping and CRISPR homology-directed repair of a recessive white eye mutation in Plodia moths

    doi: 10.1016/j.isci.2022.103885

    Figure Lengend Snippet:

    Article Snippet: Nla IV Restriction Enzyme , New England Biolabs , R0126S.

    Techniques: Recombinant, Nucleic Acid Electrophoresis, Mutagenesis, Software, Cell Culture, Sequencing