nla iv (New England Biolabs)


Structured Review

Nla Iv, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/nla iv/product/New England Biolabs
Average 94 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "Identification of roots from grass swards using PCR-RFLP and FFLP of the plastid trn L (UAA) intron"
Article Title: Identification of roots from grass swards using PCR-RFLP and FFLP of the plastid trn L (UAA) intron
Journal: BMC Ecology
doi: 10.1186/1472-6785-3-8

Figure Legend Snippet: Key to grass identification at Sourhope using trn L PCR-RFLP. Fragment sizes were determined using nucleotide sequence data of the trn L PCR product of each reference species. Restriction enzyme(s) are followed by the fragment sizes expected from digestion with the enzyme(s). GenBank accession numbers are provided in brackets following the species name.
Techniques Used: Sequencing
nla iv (New England Biolabs)


Structured Review

Nla Iv, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/nla iv/product/New England Biolabs
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "Polymorphisms within the Tumor Necrosis Factor-Alpha Gene Is Associated with Preeclampsia in Taiwanese Han Populations"
Article Title: Polymorphisms within the Tumor Necrosis Factor-Alpha Gene Is Associated with Preeclampsia in Taiwanese Han Populations
Journal: Biomedicines
doi: 10.3390/biomedicines11030862

Figure Legend Snippet: Genotype distributions and allele frequencies of the Nla IV polymorphism at the position -238 in the promoter region of the TNF-α gene among women with severe preeclampsia and normal controls.
Techniques Used:

Figure Legend Snippet: Haplotype frequencies for the Nla IV and Nco I polymorphisms at the promoter region of the TNF-a gene in severe preeclampsia and controls.
Techniques Used:
nla iv (New England Biolabs)


Structured Review
Nla Iv, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/nla iv/product/New England Biolabs
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
nla iv (New England Biolabs)


Structured Review

Nla Iv, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/nla iv/product/New England Biolabs
Average 94 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "Identification of roots from grass swards using PCR-RFLP and FFLP of the plastid trn L (UAA) intron"
Article Title: Identification of roots from grass swards using PCR-RFLP and FFLP of the plastid trn L (UAA) intron
Journal: BMC Ecology
doi: 10.1186/1472-6785-3-8

Figure Legend Snippet: Key to grass identification at Sourhope using trn L PCR-RFLP. Fragment sizes were determined using nucleotide sequence data of the trn L PCR product of each reference species. Restriction enzyme(s) are followed by the fragment sizes expected from digestion with the enzyme(s). GenBank accession numbers are provided in brackets following the species name.
Techniques Used: Sequencing
nla iv (New England Biolabs)


Structured Review

Nla Iv, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/nla iv/product/New England Biolabs
Average 94 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "Identification of roots from grass swards using PCR-RFLP and FFLP of the plastid trn L (UAA) intron"
Article Title: Identification of roots from grass swards using PCR-RFLP and FFLP of the plastid trn L (UAA) intron
Journal: BMC Ecology
doi: 10.1186/1472-6785-3-8

Figure Legend Snippet: Key to grass identification at Sourhope using trn L PCR-RFLP. Fragment sizes were determined using nucleotide sequence data of the trn L PCR product of each reference species. Restriction enzyme(s) are followed by the fragment sizes expected from digestion with the enzyme(s). GenBank accession numbers are provided in brackets following the species name.
Techniques Used: Sequencing
nla iv restriction endonuclease (New England Biolabs)


Structured Review
Nla Iv Restriction Endonuclease, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/nla iv restriction endonuclease/product/New England Biolabs
Average 95 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
nla iv (New England Biolabs)


Structured Review
Nla Iv, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/nla iv/product/New England Biolabs
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
nla iv (New England Biolabs)


Structured Review
Nla Iv, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/nla iv/product/New England Biolabs
Average 94 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
nla iv (New England Biolabs)


Structured Review

Nla Iv, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/nla iv/product/New England Biolabs
Average 94 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "Restriction and Sequence Alterations Affect DNA Uptake Sequence-Dependent Transformation in Neisseria meningitidis"
Article Title: Restriction and Sequence Alterations Affect DNA Uptake Sequence-Dependent Transformation in Neisseria meningitidis
Journal: PLoS ONE
doi: 10.1371/journal.pone.0039742

Figure Legend Snippet: Transforming DNA plasmids are based on pilG (black) interrupted by an erythromycin resistance insert, ermC , (gray). DUS ( 5′- ATGCCGTCTGAA-3′ ) or reverse complimentary DUS ( 5′-TTCAGACGGCAT-3′ ) are inserted in three different positions A, B and C as marked above the bar. All numbers refer to the nucleotide position following the start codon (1) of pilG. The 137 nucleotide long Bam HI-fragment which is removed in pDV-b, pDV-c versions is shown in white with black stripes. B. Nla IV restriction profile of rpoB and location of selective SNP. The homologous 723 nt long PCR fragment of an internal part of the meningococcal rpoB gene used in transformation contains two Nla IV restriction sites on both sides of the selective SNP responsible for rifampicin resistance in the recipient.
Techniques Used: Transformation Assay

Figure Legend Snippet: The Y axis shows the number of resistant (erythromycin) CFU/total 10 10 CFU on a log scale. Along the X-axis are the different DNA substrates (10 ng/ml) with altered numbers of Nla IV restriction sites shown. pDV4-a harbours two and three Nla IV restriction sites more than pDV4-b and pDV4-c, respectively. For further details of the restriction profiles and DUS locations in the transforming DNA plasmids please consult and . Statistically significant differences in transformation frequencies between the Nla IV null mutant and wildtype backgrounds are indicated above the columns, ***equals p≤0.001 in a two tailed paired student’s t-test.
Techniques Used: Transformation Assay, Mutagenesis, Two Tailed Test

Figure Legend Snippet: The Y axis shows the number of rifampicin-resistant CFU/total CFU 10 9 on a log scale. Along the X-axis are shown the two different DNA substrates (5 ng/ml), rpoB PCR fragment and rpoB PCR fragment pre-digested with Nla IV. For further details on the transforming DNA please consult and . The transformation frequencies in the wildtype and in the Nla IV null mutant backgrounds are not statistically significant from each other.
Techniques Used: Transformation Assay, Mutagenesis

Figure Legend Snippet: The Y axis shows the number of erythromycin-resistant CFU/total CFU 10 8 on a log scale. Along the X-axis are shown the transforming DNA (1 µg/ml) that differ in Nla IV restriction profiles and DUS location. For further details on the DNA plasmid templates please consult and . Statistically significant values are indicated above the columns, **equals p≤0.05 and ***equals p≤0.001 in a two-tailed paired student’s t tests.
Techniques Used: Plasmid Preparation, Two Tailed Test

Figure Legend Snippet: Fold-differences and statistical significance values (paired two tailed student’s t tests) in comparing the performances of individual plasmids harbouring DUS in the C (pDV1) or A (pDV4) positions in transformation of the Nla IV mutant and wt backgrounds.
Techniques Used: Two Tailed Test, Transformation Assay, Mutagenesis

Figure Legend Snippet: Plasmids and bacterial strains.
Techniques Used: Clone Assay, Plasmid Preparation, Isolation, Mutagenesis
nla iv sites (New England Biolabs)


Structured Review

Nla Iv Sites, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/nla iv sites/product/New England Biolabs
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "Restriction and Sequence Alterations Affect DNA Uptake Sequence-Dependent Transformation in Neisseria meningitidis"
Article Title: Restriction and Sequence Alterations Affect DNA Uptake Sequence-Dependent Transformation in Neisseria meningitidis
Journal: PLoS ONE
doi: 10.1371/journal.pone.0039742

Figure Legend Snippet: Transforming DNA plasmids are based on pilG (black) interrupted by an erythromycin resistance insert, ermC , (gray). DUS ( 5′- ATGCCGTCTGAA-3′ ) or reverse complimentary DUS ( 5′-TTCAGACGGCAT-3′ ) are inserted in three different positions A, B and C as marked above the bar. All numbers refer to the nucleotide position following the start codon (1) of pilG. The 137 nucleotide long Bam HI-fragment which is removed in pDV-b, pDV-c versions is shown in white with black stripes. B. Nla IV restriction profile of rpoB and location of selective SNP. The homologous 723 nt long PCR fragment of an internal part of the meningococcal rpoB gene used in transformation contains two Nla IV restriction sites on both sides of the selective SNP responsible for rifampicin resistance in the recipient.
Techniques Used: Transformation Assay

Figure Legend Snippet: The Y axis shows the number of resistant (erythromycin) CFU/total 10 10 CFU on a log scale. Along the X-axis are the different DNA substrates (10 ng/ml) with altered numbers of Nla IV restriction sites shown. pDV4-a harbours two and three Nla IV restriction sites more than pDV4-b and pDV4-c, respectively. For further details of the restriction profiles and DUS locations in the transforming DNA plasmids please consult and . Statistically significant differences in transformation frequencies between the Nla IV null mutant and wildtype backgrounds are indicated above the columns, ***equals p≤0.001 in a two tailed paired student’s t-test.
Techniques Used: Transformation Assay, Mutagenesis, Two Tailed Test

Figure Legend Snippet: The Y axis shows the number of rifampicin-resistant CFU/total CFU 10 9 on a log scale. Along the X-axis are shown the two different DNA substrates (5 ng/ml), rpoB PCR fragment and rpoB PCR fragment pre-digested with Nla IV. For further details on the transforming DNA please consult and . The transformation frequencies in the wildtype and in the Nla IV null mutant backgrounds are not statistically significant from each other.
Techniques Used: Transformation Assay, Mutagenesis

Figure Legend Snippet: The Y axis shows the number of erythromycin-resistant CFU/total CFU 10 8 on a log scale. Along the X-axis are shown the transforming DNA (1 µg/ml) that differ in Nla IV restriction profiles and DUS location. For further details on the DNA plasmid templates please consult and . Statistically significant values are indicated above the columns, **equals p≤0.05 and ***equals p≤0.001 in a two-tailed paired student’s t tests.
Techniques Used: Plasmid Preparation, Two Tailed Test

Figure Legend Snippet: Fold-differences and statistical significance values (paired two tailed student’s t tests) in comparing the performances of individual plasmids harbouring DUS in the C (pDV1) or A (pDV4) positions in transformation of the Nla IV mutant and wt backgrounds.
Techniques Used: Two Tailed Test, Transformation Assay, Mutagenesis

Figure Legend Snippet: Plasmids and bacterial strains.
Techniques Used: Clone Assay, Plasmid Preparation, Isolation, Mutagenesis
nla iv (New England Biolabs)


Structured Review
Nla Iv, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/nla iv/product/New England Biolabs
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99