nla iv  (New England Biolabs)


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    New England Biolabs nla iv
    Key to grass identification at Sourhope using trn L PCR-RFLP. Fragment sizes were determined using nucleotide sequence data of the trn L PCR product of each reference species. Restriction enzyme(s) are followed by the fragment sizes expected from digestion with the enzyme(s). GenBank accession numbers are provided in brackets following the species name.
    Nla Iv, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 94 stars, based on 1 article reviews
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    nla iv - by Bioz Stars, 2023-09
    94/100 stars

    Images

    1) Product Images from "Identification of roots from grass swards using PCR-RFLP and FFLP of the plastid trn L (UAA) intron"

    Article Title: Identification of roots from grass swards using PCR-RFLP and FFLP of the plastid trn L (UAA) intron

    Journal: BMC Ecology

    doi: 10.1186/1472-6785-3-8

    Key to grass identification at Sourhope using trn L PCR-RFLP. Fragment sizes were determined using nucleotide sequence data of the trn L PCR product of each reference species. Restriction enzyme(s) are followed by the fragment sizes expected from digestion with the enzyme(s). GenBank accession numbers are provided in brackets following the species name.
    Figure Legend Snippet: Key to grass identification at Sourhope using trn L PCR-RFLP. Fragment sizes were determined using nucleotide sequence data of the trn L PCR product of each reference species. Restriction enzyme(s) are followed by the fragment sizes expected from digestion with the enzyme(s). GenBank accession numbers are provided in brackets following the species name.

    Techniques Used: Sequencing

    nla iv  (New England Biolabs)


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    New England Biolabs nla iv
    Genotype distributions and allele frequencies of the <t> Nla IV </t> polymorphism at the position -238 in the promoter region of the TNF-α gene among women with severe preeclampsia and normal controls.
    Nla Iv, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nla iv/product/New England Biolabs
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    nla iv - by Bioz Stars, 2023-09
    86/100 stars

    Images

    1) Product Images from "Polymorphisms within the Tumor Necrosis Factor-Alpha Gene Is Associated with Preeclampsia in Taiwanese Han Populations"

    Article Title: Polymorphisms within the Tumor Necrosis Factor-Alpha Gene Is Associated with Preeclampsia in Taiwanese Han Populations

    Journal: Biomedicines

    doi: 10.3390/biomedicines11030862

    Genotype distributions and allele frequencies of the  Nla IV  polymorphism at the position -238 in the promoter region of the TNF-α gene among women with severe preeclampsia and normal controls.
    Figure Legend Snippet: Genotype distributions and allele frequencies of the Nla IV polymorphism at the position -238 in the promoter region of the TNF-α gene among women with severe preeclampsia and normal controls.

    Techniques Used:

    Haplotype frequencies for the  Nla IV  and Nco I polymorphisms at the promoter region of the TNF-a gene in severe preeclampsia and controls.
    Figure Legend Snippet: Haplotype frequencies for the Nla IV and Nco I polymorphisms at the promoter region of the TNF-a gene in severe preeclampsia and controls.

    Techniques Used:

    nla iv  (New England Biolabs)


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    New England Biolabs nla iv
    Nla Iv, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nla iv/product/New England Biolabs
    Average 86 stars, based on 1 article reviews
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    nla iv - by Bioz Stars, 2023-09
    86/100 stars

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    nla iv  (New England Biolabs)


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    New England Biolabs nla iv
    Key to grass identification at Sourhope using trn L PCR-RFLP. Fragment sizes were determined using nucleotide sequence data of the trn L PCR product of each reference species. Restriction enzyme(s) are followed by the fragment sizes expected from digestion with the enzyme(s). GenBank accession numbers are provided in brackets following the species name.
    Nla Iv, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nla iv/product/New England Biolabs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    nla iv - by Bioz Stars, 2023-09
    94/100 stars

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    1) Product Images from "Identification of roots from grass swards using PCR-RFLP and FFLP of the plastid trn L (UAA) intron"

    Article Title: Identification of roots from grass swards using PCR-RFLP and FFLP of the plastid trn L (UAA) intron

    Journal: BMC Ecology

    doi: 10.1186/1472-6785-3-8

    Key to grass identification at Sourhope using trn L PCR-RFLP. Fragment sizes were determined using nucleotide sequence data of the trn L PCR product of each reference species. Restriction enzyme(s) are followed by the fragment sizes expected from digestion with the enzyme(s). GenBank accession numbers are provided in brackets following the species name.
    Figure Legend Snippet: Key to grass identification at Sourhope using trn L PCR-RFLP. Fragment sizes were determined using nucleotide sequence data of the trn L PCR product of each reference species. Restriction enzyme(s) are followed by the fragment sizes expected from digestion with the enzyme(s). GenBank accession numbers are provided in brackets following the species name.

    Techniques Used: Sequencing

    nla iv  (New England Biolabs)


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  • 94

    Structured Review

    New England Biolabs nla iv
    Key to grass identification at Sourhope using trn L PCR-RFLP. Fragment sizes were determined using nucleotide sequence data of the trn L PCR product of each reference species. Restriction enzyme(s) are followed by the fragment sizes expected from digestion with the enzyme(s). GenBank accession numbers are provided in brackets following the species name.
    Nla Iv, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nla iv/product/New England Biolabs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    nla iv - by Bioz Stars, 2023-09
    94/100 stars

    Images

    1) Product Images from "Identification of roots from grass swards using PCR-RFLP and FFLP of the plastid trn L (UAA) intron"

    Article Title: Identification of roots from grass swards using PCR-RFLP and FFLP of the plastid trn L (UAA) intron

    Journal: BMC Ecology

    doi: 10.1186/1472-6785-3-8

    Key to grass identification at Sourhope using trn L PCR-RFLP. Fragment sizes were determined using nucleotide sequence data of the trn L PCR product of each reference species. Restriction enzyme(s) are followed by the fragment sizes expected from digestion with the enzyme(s). GenBank accession numbers are provided in brackets following the species name.
    Figure Legend Snippet: Key to grass identification at Sourhope using trn L PCR-RFLP. Fragment sizes were determined using nucleotide sequence data of the trn L PCR product of each reference species. Restriction enzyme(s) are followed by the fragment sizes expected from digestion with the enzyme(s). GenBank accession numbers are provided in brackets following the species name.

    Techniques Used: Sequencing

    nla iv restriction endonuclease  (New England Biolabs)


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    New England Biolabs nla iv restriction endonuclease
    Nla Iv Restriction Endonuclease, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    nla iv  (New England Biolabs)


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    New England Biolabs nla iv
    Nla Iv, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    nla iv  (New England Biolabs)


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    New England Biolabs nla iv
    Nla Iv, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    nla iv  (New England Biolabs)


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    New England Biolabs nla iv
    Transforming DNA plasmids are based on pilG (black) interrupted by an erythromycin resistance insert, ermC , (gray). DUS ( 5′- ATGCCGTCTGAA-3′ ) or reverse complimentary DUS ( 5′-TTCAGACGGCAT-3′ ) are inserted in three different positions A, B and C as marked above the bar. All numbers refer to the nucleotide position following the start codon (1) of pilG. The 137 nucleotide long Bam HI-fragment which is removed in pDV-b, pDV-c versions is shown in white with black stripes. B. <t>Nla</t> IV restriction profile <t>of</t> <t>rpoB</t> and location of selective SNP. The homologous 723 nt long PCR fragment of an internal part of the meningococcal rpoB gene used in transformation contains two Nla IV restriction sites on both sides of the selective SNP responsible for rifampicin resistance in the recipient.
    Nla Iv, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nla iv/product/New England Biolabs
    Average 94 stars, based on 1 article reviews
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    Images

    1) Product Images from "Restriction and Sequence Alterations Affect DNA Uptake Sequence-Dependent Transformation in Neisseria meningitidis"

    Article Title: Restriction and Sequence Alterations Affect DNA Uptake Sequence-Dependent Transformation in Neisseria meningitidis

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0039742

    Transforming DNA plasmids are based on pilG (black) interrupted by an erythromycin resistance insert, ermC , (gray). DUS ( 5′- ATGCCGTCTGAA-3′ ) or reverse complimentary DUS ( 5′-TTCAGACGGCAT-3′ ) are inserted in three different positions A, B and C as marked above the bar. All numbers refer to the nucleotide position following the start codon (1) of pilG. The 137 nucleotide long Bam HI-fragment which is removed in pDV-b, pDV-c versions is shown in white with black stripes. B. Nla IV restriction profile of rpoB and location of selective SNP. The homologous 723 nt long PCR fragment of an internal part of the meningococcal rpoB gene used in transformation contains two Nla IV restriction sites on both sides of the selective SNP responsible for rifampicin resistance in the recipient.
    Figure Legend Snippet: Transforming DNA plasmids are based on pilG (black) interrupted by an erythromycin resistance insert, ermC , (gray). DUS ( 5′- ATGCCGTCTGAA-3′ ) or reverse complimentary DUS ( 5′-TTCAGACGGCAT-3′ ) are inserted in three different positions A, B and C as marked above the bar. All numbers refer to the nucleotide position following the start codon (1) of pilG. The 137 nucleotide long Bam HI-fragment which is removed in pDV-b, pDV-c versions is shown in white with black stripes. B. Nla IV restriction profile of rpoB and location of selective SNP. The homologous 723 nt long PCR fragment of an internal part of the meningococcal rpoB gene used in transformation contains two Nla IV restriction sites on both sides of the selective SNP responsible for rifampicin resistance in the recipient.

    Techniques Used: Transformation Assay

    The Y axis shows the number of resistant (erythromycin) CFU/total 10 10 CFU on a log scale. Along the X-axis are the different DNA substrates (10 ng/ml) with altered numbers of Nla IV restriction sites shown. pDV4-a harbours two and three Nla IV restriction sites more than pDV4-b and pDV4-c, respectively. For further details of the restriction profiles and DUS locations in the transforming DNA plasmids please consult and . Statistically significant differences in transformation frequencies between the Nla IV null mutant and wildtype backgrounds are indicated above the columns, ***equals p≤0.001 in a two tailed paired student’s t-test.
    Figure Legend Snippet: The Y axis shows the number of resistant (erythromycin) CFU/total 10 10 CFU on a log scale. Along the X-axis are the different DNA substrates (10 ng/ml) with altered numbers of Nla IV restriction sites shown. pDV4-a harbours two and three Nla IV restriction sites more than pDV4-b and pDV4-c, respectively. For further details of the restriction profiles and DUS locations in the transforming DNA plasmids please consult and . Statistically significant differences in transformation frequencies between the Nla IV null mutant and wildtype backgrounds are indicated above the columns, ***equals p≤0.001 in a two tailed paired student’s t-test.

    Techniques Used: Transformation Assay, Mutagenesis, Two Tailed Test

    The Y axis shows the number of rifampicin-resistant CFU/total CFU 10 9 on a log scale. Along the X-axis are shown the two different DNA substrates (5 ng/ml), rpoB PCR fragment and rpoB PCR fragment pre-digested with Nla IV. For further details on the transforming DNA please consult and . The transformation frequencies in the wildtype and in the Nla IV null mutant backgrounds are not statistically significant from each other.
    Figure Legend Snippet: The Y axis shows the number of rifampicin-resistant CFU/total CFU 10 9 on a log scale. Along the X-axis are shown the two different DNA substrates (5 ng/ml), rpoB PCR fragment and rpoB PCR fragment pre-digested with Nla IV. For further details on the transforming DNA please consult and . The transformation frequencies in the wildtype and in the Nla IV null mutant backgrounds are not statistically significant from each other.

    Techniques Used: Transformation Assay, Mutagenesis

    The Y axis shows the number of erythromycin-resistant CFU/total CFU 10 8 on a log scale. Along the X-axis are shown the transforming DNA (1 µg/ml) that differ in Nla IV restriction profiles and DUS location. For further details on the DNA plasmid templates please consult and . Statistically significant values are indicated above the columns, **equals p≤0.05 and ***equals p≤0.001 in a two-tailed paired student’s t tests.
    Figure Legend Snippet: The Y axis shows the number of erythromycin-resistant CFU/total CFU 10 8 on a log scale. Along the X-axis are shown the transforming DNA (1 µg/ml) that differ in Nla IV restriction profiles and DUS location. For further details on the DNA plasmid templates please consult and . Statistically significant values are indicated above the columns, **equals p≤0.05 and ***equals p≤0.001 in a two-tailed paired student’s t tests.

    Techniques Used: Plasmid Preparation, Two Tailed Test

    Fold-differences and statistical significance values (paired two tailed student’s t tests) in comparing the performances of individual plasmids harbouring DUS in the C (pDV1) or A (pDV4) positions in transformation of the  Nla IV  mutant and wt backgrounds.
    Figure Legend Snippet: Fold-differences and statistical significance values (paired two tailed student’s t tests) in comparing the performances of individual plasmids harbouring DUS in the C (pDV1) or A (pDV4) positions in transformation of the Nla IV mutant and wt backgrounds.

    Techniques Used: Two Tailed Test, Transformation Assay, Mutagenesis

    Plasmids and bacterial strains.
    Figure Legend Snippet: Plasmids and bacterial strains.

    Techniques Used: Clone Assay, Plasmid Preparation, Isolation, Mutagenesis

    nla iv sites  (New England Biolabs)


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    New England Biolabs nla iv sites
    Transforming DNA plasmids are based on pilG (black) interrupted by an erythromycin resistance insert, ermC , <t>(gray).</t> <t>DUS</t> ( 5′- ATGCCGTCTGAA-3′ ) or reverse complimentary DUS ( 5′-TTCAGACGGCAT-3′ ) are inserted in three different positions A, B and C as marked above the bar. All numbers refer to the nucleotide position following the start codon (1) of pilG. The 137 nucleotide long Bam HI-fragment which is removed in pDV-b, pDV-c versions is shown in white with black stripes. B. <t>Nla</t> IV restriction profile of rpoB and location of selective SNP. The homologous 723 nt long PCR fragment of an internal part of the meningococcal rpoB gene used in transformation contains two Nla IV restriction sites on both sides of the selective SNP responsible for rifampicin resistance in the recipient.
    Nla Iv Sites, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nla iv sites/product/New England Biolabs
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    Price from $9.99 to $1999.99
    nla iv sites - by Bioz Stars, 2023-09
    86/100 stars

    Images

    1) Product Images from "Restriction and Sequence Alterations Affect DNA Uptake Sequence-Dependent Transformation in Neisseria meningitidis"

    Article Title: Restriction and Sequence Alterations Affect DNA Uptake Sequence-Dependent Transformation in Neisseria meningitidis

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0039742

    Transforming DNA plasmids are based on pilG (black) interrupted by an erythromycin resistance insert, ermC , (gray). DUS ( 5′- ATGCCGTCTGAA-3′ ) or reverse complimentary DUS ( 5′-TTCAGACGGCAT-3′ ) are inserted in three different positions A, B and C as marked above the bar. All numbers refer to the nucleotide position following the start codon (1) of pilG. The 137 nucleotide long Bam HI-fragment which is removed in pDV-b, pDV-c versions is shown in white with black stripes. B. Nla IV restriction profile of rpoB and location of selective SNP. The homologous 723 nt long PCR fragment of an internal part of the meningococcal rpoB gene used in transformation contains two Nla IV restriction sites on both sides of the selective SNP responsible for rifampicin resistance in the recipient.
    Figure Legend Snippet: Transforming DNA plasmids are based on pilG (black) interrupted by an erythromycin resistance insert, ermC , (gray). DUS ( 5′- ATGCCGTCTGAA-3′ ) or reverse complimentary DUS ( 5′-TTCAGACGGCAT-3′ ) are inserted in three different positions A, B and C as marked above the bar. All numbers refer to the nucleotide position following the start codon (1) of pilG. The 137 nucleotide long Bam HI-fragment which is removed in pDV-b, pDV-c versions is shown in white with black stripes. B. Nla IV restriction profile of rpoB and location of selective SNP. The homologous 723 nt long PCR fragment of an internal part of the meningococcal rpoB gene used in transformation contains two Nla IV restriction sites on both sides of the selective SNP responsible for rifampicin resistance in the recipient.

    Techniques Used: Transformation Assay

    The Y axis shows the number of resistant (erythromycin) CFU/total 10 10 CFU on a log scale. Along the X-axis are the different DNA substrates (10 ng/ml) with altered numbers of Nla IV restriction sites shown. pDV4-a harbours two and three Nla IV restriction sites more than pDV4-b and pDV4-c, respectively. For further details of the restriction profiles and DUS locations in the transforming DNA plasmids please consult and . Statistically significant differences in transformation frequencies between the Nla IV null mutant and wildtype backgrounds are indicated above the columns, ***equals p≤0.001 in a two tailed paired student’s t-test.
    Figure Legend Snippet: The Y axis shows the number of resistant (erythromycin) CFU/total 10 10 CFU on a log scale. Along the X-axis are the different DNA substrates (10 ng/ml) with altered numbers of Nla IV restriction sites shown. pDV4-a harbours two and three Nla IV restriction sites more than pDV4-b and pDV4-c, respectively. For further details of the restriction profiles and DUS locations in the transforming DNA plasmids please consult and . Statistically significant differences in transformation frequencies between the Nla IV null mutant and wildtype backgrounds are indicated above the columns, ***equals p≤0.001 in a two tailed paired student’s t-test.

    Techniques Used: Transformation Assay, Mutagenesis, Two Tailed Test

    The Y axis shows the number of rifampicin-resistant CFU/total CFU 10 9 on a log scale. Along the X-axis are shown the two different DNA substrates (5 ng/ml), rpoB PCR fragment and rpoB PCR fragment pre-digested with Nla IV. For further details on the transforming DNA please consult and . The transformation frequencies in the wildtype and in the Nla IV null mutant backgrounds are not statistically significant from each other.
    Figure Legend Snippet: The Y axis shows the number of rifampicin-resistant CFU/total CFU 10 9 on a log scale. Along the X-axis are shown the two different DNA substrates (5 ng/ml), rpoB PCR fragment and rpoB PCR fragment pre-digested with Nla IV. For further details on the transforming DNA please consult and . The transformation frequencies in the wildtype and in the Nla IV null mutant backgrounds are not statistically significant from each other.

    Techniques Used: Transformation Assay, Mutagenesis

    The Y axis shows the number of erythromycin-resistant CFU/total CFU 10 8 on a log scale. Along the X-axis are shown the transforming DNA (1 µg/ml) that differ in Nla IV restriction profiles and DUS location. For further details on the DNA plasmid templates please consult and . Statistically significant values are indicated above the columns, **equals p≤0.05 and ***equals p≤0.001 in a two-tailed paired student’s t tests.
    Figure Legend Snippet: The Y axis shows the number of erythromycin-resistant CFU/total CFU 10 8 on a log scale. Along the X-axis are shown the transforming DNA (1 µg/ml) that differ in Nla IV restriction profiles and DUS location. For further details on the DNA plasmid templates please consult and . Statistically significant values are indicated above the columns, **equals p≤0.05 and ***equals p≤0.001 in a two-tailed paired student’s t tests.

    Techniques Used: Plasmid Preparation, Two Tailed Test

    Fold-differences and statistical significance values (paired two tailed student’s t tests) in comparing the performances of individual plasmids harbouring  DUS  in the C (pDV1) or A (pDV4) positions in transformation of the  Nla IV  mutant and wt backgrounds.
    Figure Legend Snippet: Fold-differences and statistical significance values (paired two tailed student’s t tests) in comparing the performances of individual plasmids harbouring DUS in the C (pDV1) or A (pDV4) positions in transformation of the Nla IV mutant and wt backgrounds.

    Techniques Used: Two Tailed Test, Transformation Assay, Mutagenesis

    Plasmids and bacterial strains.
    Figure Legend Snippet: Plasmids and bacterial strains.

    Techniques Used: Clone Assay, Plasmid Preparation, Isolation, Mutagenesis

    nla iv  (New England Biolabs)


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    New England Biolabs nla iv
    Nla Iv, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86/100 stars

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    New England Biolabs nla iv
    Key to grass identification at Sourhope using trn L PCR-RFLP. Fragment sizes were determined using nucleotide sequence data of the trn L PCR product of each reference species. Restriction enzyme(s) are followed by the fragment sizes expected from digestion with the enzyme(s). GenBank accession numbers are provided in brackets following the species name.
    Nla Iv, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nla iv/product/New England Biolabs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    nla iv - by Bioz Stars, 2023-09
    94/100 stars
      Buy from Supplier

    95
    New England Biolabs nla iv restriction endonuclease
    Key to grass identification at Sourhope using trn L PCR-RFLP. Fragment sizes were determined using nucleotide sequence data of the trn L PCR product of each reference species. Restriction enzyme(s) are followed by the fragment sizes expected from digestion with the enzyme(s). GenBank accession numbers are provided in brackets following the species name.
    Nla Iv Restriction Endonuclease, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nla iv restriction endonuclease/product/New England Biolabs
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    nla iv restriction endonuclease - by Bioz Stars, 2023-09
    95/100 stars
      Buy from Supplier

    86
    New England Biolabs nla iv sites
    Transforming DNA plasmids are based on pilG (black) interrupted by an erythromycin resistance insert, ermC , <t>(gray).</t> <t>DUS</t> ( 5′- ATGCCGTCTGAA-3′ ) or reverse complimentary DUS ( 5′-TTCAGACGGCAT-3′ ) are inserted in three different positions A, B and C as marked above the bar. All numbers refer to the nucleotide position following the start codon (1) of pilG. The 137 nucleotide long Bam HI-fragment which is removed in pDV-b, pDV-c versions is shown in white with black stripes. B. <t>Nla</t> IV restriction profile of rpoB and location of selective SNP. The homologous 723 nt long PCR fragment of an internal part of the meningococcal rpoB gene used in transformation contains two Nla IV restriction sites on both sides of the selective SNP responsible for rifampicin resistance in the recipient.
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    Key to grass identification at Sourhope using trn L PCR-RFLP. Fragment sizes were determined using nucleotide sequence data of the trn L PCR product of each reference species. Restriction enzyme(s) are followed by the fragment sizes expected from digestion with the enzyme(s). GenBank accession numbers are provided in brackets following the species name.

    Journal: BMC Ecology

    Article Title: Identification of roots from grass swards using PCR-RFLP and FFLP of the plastid trn L (UAA) intron

    doi: 10.1186/1472-6785-3-8

    Figure Lengend Snippet: Key to grass identification at Sourhope using trn L PCR-RFLP. Fragment sizes were determined using nucleotide sequence data of the trn L PCR product of each reference species. Restriction enzyme(s) are followed by the fragment sizes expected from digestion with the enzyme(s). GenBank accession numbers are provided in brackets following the species name.

    Article Snippet: The double digest with Nla IV and Dde I was performed in Promega buffer B. Bfa I and Nla IV were obtained from New England Biolabs; Dde I, Hin fI, Hsp 92II and Xmn I from Promega.

    Techniques: Sequencing

    Transforming DNA plasmids are based on pilG (black) interrupted by an erythromycin resistance insert, ermC , (gray). DUS ( 5′- ATGCCGTCTGAA-3′ ) or reverse complimentary DUS ( 5′-TTCAGACGGCAT-3′ ) are inserted in three different positions A, B and C as marked above the bar. All numbers refer to the nucleotide position following the start codon (1) of pilG. The 137 nucleotide long Bam HI-fragment which is removed in pDV-b, pDV-c versions is shown in white with black stripes. B. Nla IV restriction profile of rpoB and location of selective SNP. The homologous 723 nt long PCR fragment of an internal part of the meningococcal rpoB gene used in transformation contains two Nla IV restriction sites on both sides of the selective SNP responsible for rifampicin resistance in the recipient.

    Journal: PLoS ONE

    Article Title: Restriction and Sequence Alterations Affect DNA Uptake Sequence-Dependent Transformation in Neisseria meningitidis

    doi: 10.1371/journal.pone.0039742

    Figure Lengend Snippet: Transforming DNA plasmids are based on pilG (black) interrupted by an erythromycin resistance insert, ermC , (gray). DUS ( 5′- ATGCCGTCTGAA-3′ ) or reverse complimentary DUS ( 5′-TTCAGACGGCAT-3′ ) are inserted in three different positions A, B and C as marked above the bar. All numbers refer to the nucleotide position following the start codon (1) of pilG. The 137 nucleotide long Bam HI-fragment which is removed in pDV-b, pDV-c versions is shown in white with black stripes. B. Nla IV restriction profile of rpoB and location of selective SNP. The homologous 723 nt long PCR fragment of an internal part of the meningococcal rpoB gene used in transformation contains two Nla IV restriction sites on both sides of the selective SNP responsible for rifampicin resistance in the recipient.

    Article Snippet: The hybrid plasmids pOHA-0-DUS and pOHA-Single from a previous study containing the naturally DUS-less neisserial pilus biogenesis gene pilG harbouring a selective marker encoding erythromycin (erm) resistance , were used to make all constructs employed, varying in DUS, Nla IV sites and homologous region as shown in . pOHA-0-DUS and pSingle were trimmed with Bam HI (recognition sequence GGATCC) (New England Biolabs) and recircularised with T4 Ligase (New England Biolabs) or polished with Phusion polymerase (New England Biolabs) following Bam HI restriction and recircularised to generate the plasmids -b, -c and -d versions of pOHA-0-DUS and pOHA13, respectively.

    Techniques: Transformation Assay

    The Y axis shows the number of resistant (erythromycin) CFU/total 10 10 CFU on a log scale. Along the X-axis are the different DNA substrates (10 ng/ml) with altered numbers of Nla IV restriction sites shown. pDV4-a harbours two and three Nla IV restriction sites more than pDV4-b and pDV4-c, respectively. For further details of the restriction profiles and DUS locations in the transforming DNA plasmids please consult and . Statistically significant differences in transformation frequencies between the Nla IV null mutant and wildtype backgrounds are indicated above the columns, ***equals p≤0.001 in a two tailed paired student’s t-test.

    Journal: PLoS ONE

    Article Title: Restriction and Sequence Alterations Affect DNA Uptake Sequence-Dependent Transformation in Neisseria meningitidis

    doi: 10.1371/journal.pone.0039742

    Figure Lengend Snippet: The Y axis shows the number of resistant (erythromycin) CFU/total 10 10 CFU on a log scale. Along the X-axis are the different DNA substrates (10 ng/ml) with altered numbers of Nla IV restriction sites shown. pDV4-a harbours two and three Nla IV restriction sites more than pDV4-b and pDV4-c, respectively. For further details of the restriction profiles and DUS locations in the transforming DNA plasmids please consult and . Statistically significant differences in transformation frequencies between the Nla IV null mutant and wildtype backgrounds are indicated above the columns, ***equals p≤0.001 in a two tailed paired student’s t-test.

    Article Snippet: The hybrid plasmids pOHA-0-DUS and pOHA-Single from a previous study containing the naturally DUS-less neisserial pilus biogenesis gene pilG harbouring a selective marker encoding erythromycin (erm) resistance , were used to make all constructs employed, varying in DUS, Nla IV sites and homologous region as shown in . pOHA-0-DUS and pSingle were trimmed with Bam HI (recognition sequence GGATCC) (New England Biolabs) and recircularised with T4 Ligase (New England Biolabs) or polished with Phusion polymerase (New England Biolabs) following Bam HI restriction and recircularised to generate the plasmids -b, -c and -d versions of pOHA-0-DUS and pOHA13, respectively.

    Techniques: Transformation Assay, Mutagenesis, Two Tailed Test

    The Y axis shows the number of rifampicin-resistant CFU/total CFU 10 9 on a log scale. Along the X-axis are shown the two different DNA substrates (5 ng/ml), rpoB PCR fragment and rpoB PCR fragment pre-digested with Nla IV. For further details on the transforming DNA please consult and . The transformation frequencies in the wildtype and in the Nla IV null mutant backgrounds are not statistically significant from each other.

    Journal: PLoS ONE

    Article Title: Restriction and Sequence Alterations Affect DNA Uptake Sequence-Dependent Transformation in Neisseria meningitidis

    doi: 10.1371/journal.pone.0039742

    Figure Lengend Snippet: The Y axis shows the number of rifampicin-resistant CFU/total CFU 10 9 on a log scale. Along the X-axis are shown the two different DNA substrates (5 ng/ml), rpoB PCR fragment and rpoB PCR fragment pre-digested with Nla IV. For further details on the transforming DNA please consult and . The transformation frequencies in the wildtype and in the Nla IV null mutant backgrounds are not statistically significant from each other.

    Article Snippet: The hybrid plasmids pOHA-0-DUS and pOHA-Single from a previous study containing the naturally DUS-less neisserial pilus biogenesis gene pilG harbouring a selective marker encoding erythromycin (erm) resistance , were used to make all constructs employed, varying in DUS, Nla IV sites and homologous region as shown in . pOHA-0-DUS and pSingle were trimmed with Bam HI (recognition sequence GGATCC) (New England Biolabs) and recircularised with T4 Ligase (New England Biolabs) or polished with Phusion polymerase (New England Biolabs) following Bam HI restriction and recircularised to generate the plasmids -b, -c and -d versions of pOHA-0-DUS and pOHA13, respectively.

    Techniques: Transformation Assay, Mutagenesis

    The Y axis shows the number of erythromycin-resistant CFU/total CFU 10 8 on a log scale. Along the X-axis are shown the transforming DNA (1 µg/ml) that differ in Nla IV restriction profiles and DUS location. For further details on the DNA plasmid templates please consult and . Statistically significant values are indicated above the columns, **equals p≤0.05 and ***equals p≤0.001 in a two-tailed paired student’s t tests.

    Journal: PLoS ONE

    Article Title: Restriction and Sequence Alterations Affect DNA Uptake Sequence-Dependent Transformation in Neisseria meningitidis

    doi: 10.1371/journal.pone.0039742

    Figure Lengend Snippet: The Y axis shows the number of erythromycin-resistant CFU/total CFU 10 8 on a log scale. Along the X-axis are shown the transforming DNA (1 µg/ml) that differ in Nla IV restriction profiles and DUS location. For further details on the DNA plasmid templates please consult and . Statistically significant values are indicated above the columns, **equals p≤0.05 and ***equals p≤0.001 in a two-tailed paired student’s t tests.

    Article Snippet: The hybrid plasmids pOHA-0-DUS and pOHA-Single from a previous study containing the naturally DUS-less neisserial pilus biogenesis gene pilG harbouring a selective marker encoding erythromycin (erm) resistance , were used to make all constructs employed, varying in DUS, Nla IV sites and homologous region as shown in . pOHA-0-DUS and pSingle were trimmed with Bam HI (recognition sequence GGATCC) (New England Biolabs) and recircularised with T4 Ligase (New England Biolabs) or polished with Phusion polymerase (New England Biolabs) following Bam HI restriction and recircularised to generate the plasmids -b, -c and -d versions of pOHA-0-DUS and pOHA13, respectively.

    Techniques: Plasmid Preparation, Two Tailed Test

    Fold-differences and statistical significance values (paired two tailed student’s t tests) in comparing the performances of individual plasmids harbouring  DUS  in the C (pDV1) or A (pDV4) positions in transformation of the  Nla IV  mutant and wt backgrounds.

    Journal: PLoS ONE

    Article Title: Restriction and Sequence Alterations Affect DNA Uptake Sequence-Dependent Transformation in Neisseria meningitidis

    doi: 10.1371/journal.pone.0039742

    Figure Lengend Snippet: Fold-differences and statistical significance values (paired two tailed student’s t tests) in comparing the performances of individual plasmids harbouring DUS in the C (pDV1) or A (pDV4) positions in transformation of the Nla IV mutant and wt backgrounds.

    Article Snippet: The hybrid plasmids pOHA-0-DUS and pOHA-Single from a previous study containing the naturally DUS-less neisserial pilus biogenesis gene pilG harbouring a selective marker encoding erythromycin (erm) resistance , were used to make all constructs employed, varying in DUS, Nla IV sites and homologous region as shown in . pOHA-0-DUS and pSingle were trimmed with Bam HI (recognition sequence GGATCC) (New England Biolabs) and recircularised with T4 Ligase (New England Biolabs) or polished with Phusion polymerase (New England Biolabs) following Bam HI restriction and recircularised to generate the plasmids -b, -c and -d versions of pOHA-0-DUS and pOHA13, respectively.

    Techniques: Two Tailed Test, Transformation Assay, Mutagenesis

    Plasmids and bacterial strains.

    Journal: PLoS ONE

    Article Title: Restriction and Sequence Alterations Affect DNA Uptake Sequence-Dependent Transformation in Neisseria meningitidis

    doi: 10.1371/journal.pone.0039742

    Figure Lengend Snippet: Plasmids and bacterial strains.

    Article Snippet: The hybrid plasmids pOHA-0-DUS and pOHA-Single from a previous study containing the naturally DUS-less neisserial pilus biogenesis gene pilG harbouring a selective marker encoding erythromycin (erm) resistance , were used to make all constructs employed, varying in DUS, Nla IV sites and homologous region as shown in . pOHA-0-DUS and pSingle were trimmed with Bam HI (recognition sequence GGATCC) (New England Biolabs) and recircularised with T4 Ligase (New England Biolabs) or polished with Phusion polymerase (New England Biolabs) following Bam HI restriction and recircularised to generate the plasmids -b, -c and -d versions of pOHA-0-DUS and pOHA13, respectively.

    Techniques: Clone Assay, Plasmid Preparation, Isolation, Mutagenesis