nla iv (New England Biolabs)


Structured Review

Nla Iv, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/nla iv/product/New England Biolabs
Average 94 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "Restriction and Sequence Alterations Affect DNA Uptake Sequence-Dependent Transformation in Neisseria meningitidis"
Article Title: Restriction and Sequence Alterations Affect DNA Uptake Sequence-Dependent Transformation in Neisseria meningitidis
Journal: PLoS ONE
doi: 10.1371/journal.pone.0039742

Figure Legend Snippet: Sequence characteristics of transforming DNA. A. Variable DUS positions and restriction profiles in transforming DNA plasmids. Transforming DNA plasmids are based on pilG (black) interrupted by an erythromycin resistance insert, ermC , (gray). DUS ( 5′- ATGCCGTCTGAA-3′ ) or reverse complimentary DUS ( 5′-TTCAGACGGCAT-3′ ) are inserted in three different positions A, B and C as marked above the bar. All numbers refer to the nucleotide position following the start codon (1) of pilG. The 137 nucleotide long Bam HI-fragment which is removed in pDV-b, pDV-c versions is shown in white with black stripes. B. Nla IV restriction profile of rpoB and location of selective SNP. The homologous 723 nt long PCR fragment of an internal part of the meningococcal rpoB gene used in transformation contains two Nla IV restriction sites on both sides of the selective SNP responsible for rifampicin resistance in the recipient.
Techniques Used: Sequencing, Polymerase Chain Reaction, Transformation Assay

Figure Legend Snippet: Nla IV restriction does not affect homologous DNA transformation in N. meningitidis MC58 wildtype or in the Nla IV null mutant. The Y axis shows the number of rifampicin-resistant CFU/total CFU 10 9 on a log scale. Along the X-axis are shown the two different DNA substrates (5 ng/ml), rpoB PCR fragment and rpoB PCR fragment pre-digested with Nla IV. For further details on the transforming DNA please consult Figure 1 and Table 2 . The transformation frequencies in the wildtype and in the Nla IV null mutant backgrounds are not statistically significant from each other.
Techniques Used: Transformation Assay, Mutagenesis, Polymerase Chain Reaction