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Illumina Inc nla iii
Nla Iii, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 89/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/nla iii/product/Illumina Inc
Average 89 stars, based on 4 article reviews
Price from $9.99 to $1999.99
nla iii - by Bioz Stars, 2019-10
89/100 stars

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Amplification:

Article Title: Transcriptome differences in the hypopharyngeal gland between Western Honeybees (Apis mellifera) and Eastern Honeybees (Apis cerana)
Article Snippet: The cDNA was digested with Nla III which could recognize CATG site and the Illumina adaptor 1 was ligated to the sticky 5′ end of the digested bead-bound 3′ cDNA fragments. .. The cDNA was digested with Nla III which could recognize CATG site and the Illumina adaptor 1 was ligated to the sticky 5′ end of the digested bead-bound 3′ cDNA fragments.

Article Title: Transcriptome assembly and expression profiling of molecular responses to cadmium toxicity in hepatopancreas of the freshwater crab Sinopotamon henanense
Article Snippet: Subsequently, the bead-bound cDNA was digested with Nla III and added Illumina adapter 1, and then digested with Mme I and added Illumina adapter 2 to create the sequencing library. .. After amplification by PCR, cDNA tag library was purified and checked.

Synthesized:

Article Title: Transcriptome differences in the hypopharyngeal gland between Western Honeybees (Apis mellifera) and Eastern Honeybees (Apis cerana)
Article Snippet: Briefly, mRNA was purified from 6 μg of total RNA with oligo (dT) magnetic beads and then synthesized into double-stranded DNA (cDNA) by reverse transcription. .. The cDNA was digested with Nla III which could recognize CATG site and the Illumina adaptor 1 was ligated to the sticky 5′ end of the digested bead-bound 3′ cDNA fragments.

Construct:

Article Title: Transcriptome differences in the hypopharyngeal gland between Western Honeybees (Apis mellifera) and Eastern Honeybees (Apis cerana)
Article Snippet: The tag-seq libraries were then constructed using the Illumina Gene Expression Sample Prep Kit according to the manufacturer’s instruction. .. The cDNA was digested with Nla III which could recognize CATG site and the Illumina adaptor 1 was ligated to the sticky 5′ end of the digested bead-bound 3′ cDNA fragments.

Article Title: Transcriptome assembly and expression profiling of molecular responses to cadmium toxicity in hepatopancreas of the freshwater crab Sinopotamon henanense
Article Snippet: The separately stored 4 RNA samples from the control group and three Cd treatment groups (n = 3 in each group) were respectively used to isolate the mRNAs by oligo (dT) beads and Oligotex mRNA Kits (Qiagen, Valencia, CA, USA), and the isolated mRNAs were used to construct 4 differential cDNA libraries in parallel with Digital Gene Expression (DGE) tag profile kit (Illumina, San Diego, CA, USA), according to the manufacturer’s protocol (Version 2.1B). .. Subsequently, the bead-bound cDNA was digested with Nla III and added Illumina adapter 1, and then digested with Mme I and added Illumina adapter 2 to create the sequencing library.

Formalin-fixed Paraffin-Embedded:

Article Title: Histone methylation mediates plasticity of human FOXP3+ regulatory T cells by modulating signature gene expressions
Article Snippet: Total RNA was extracted from fixed cells using High pure FFPE RNA Micro Kit (Roche Diagnostic Corp., Indianapolis, IN) and submitted to BGI-Shenzhen (China) for lllumina sequencing. .. The resultant cDNA was endonuclease digested ( Nla III), which recognizes and cuts the most 3′ ‘CATG’, and ligated to Illumina adapter 1.

Expressing:

Article Title: Low Temperature Mitigates Cardia Bifida in Zebrafish Embryos
Article Snippet: Paragraph title: Digital Gene Expression (DGE) Analysis ... The cDNA was digested with Nla III and ligated to Illumina adaptor 1.

Article Title: Transcriptome differences in the hypopharyngeal gland between Western Honeybees (Apis mellifera) and Eastern Honeybees (Apis cerana)
Article Snippet: Paragraph title: Digital gene expression library preparation and sequencing ... The cDNA was digested with Nla III which could recognize CATG site and the Illumina adaptor 1 was ligated to the sticky 5′ end of the digested bead-bound 3′ cDNA fragments.

Article Title: Transcriptome assembly and expression profiling of molecular responses to cadmium toxicity in hepatopancreas of the freshwater crab Sinopotamon henanense
Article Snippet: Paragraph title: Differential expression library construction, gene expression profile sequencing and annotation ... Subsequently, the bead-bound cDNA was digested with Nla III and added Illumina adapter 1, and then digested with Mme I and added Illumina adapter 2 to create the sequencing library.

Article Title: Histone methylation mediates plasticity of human FOXP3+ regulatory T cells by modulating signature gene expressions
Article Snippet: Paragraph title: DGE and differential expression analysis ... The resultant cDNA was endonuclease digested ( Nla III), which recognizes and cuts the most 3′ ‘CATG’, and ligated to Illumina adapter 1.

Transformation Assay:

Article Title: Transcriptome assembly and expression profiling of molecular responses to cadmium toxicity in hepatopancreas of the freshwater crab Sinopotamon henanense
Article Snippet: Subsequently, the bead-bound cDNA was digested with Nla III and added Illumina adapter 1, and then digested with Mme I and added Illumina adapter 2 to create the sequencing library. .. Subsequently, the bead-bound cDNA was digested with Nla III and added Illumina adapter 1, and then digested with Mme I and added Illumina adapter 2 to create the sequencing library.

Article Title: Histone methylation mediates plasticity of human FOXP3+ regulatory T cells by modulating signature gene expressions
Article Snippet: The resultant cDNA was endonuclease digested ( Nla III), which recognizes and cuts the most 3′ ‘CATG’, and ligated to Illumina adapter 1. .. Following a sequential digestion of the 5′ end with Mme I and ligation to Illumina adapter 2, the DNA fragments (library) were amplified by PCR, gel purified and sequenced on the HiSeq™ 2000 system.

Flow Cytometry:

Article Title: Transcriptome assembly and expression profiling of molecular responses to cadmium toxicity in hepatopancreas of the freshwater crab Sinopotamon henanense
Article Snippet: Subsequently, the bead-bound cDNA was digested with Nla III and added Illumina adapter 1, and then digested with Mme I and added Illumina adapter 2 to create the sequencing library. .. After amplification by PCR, cDNA tag library was purified and checked.

Polymerase Chain Reaction:

Article Title: Low Temperature Mitigates Cardia Bifida in Zebrafish Embryos
Article Snippet: The cDNA was digested with Nla III and ligated to Illumina adaptor 1. .. The cDNA was digested with Nla III and ligated to Illumina adaptor 1.

Article Title: Transcriptome differences in the hypopharyngeal gland between Western Honeybees (Apis mellifera) and Eastern Honeybees (Apis cerana)
Article Snippet: The cDNA was digested with Nla III which could recognize CATG site and the Illumina adaptor 1 was ligated to the sticky 5′ end of the digested bead-bound 3′ cDNA fragments. .. The cDNA was digested with Nla III which could recognize CATG site and the Illumina adaptor 1 was ligated to the sticky 5′ end of the digested bead-bound 3′ cDNA fragments.

Article Title: Genome-Wide Mapping of DNA Methylation in Chicken
Article Snippet: The mRNAs were isolated and reverse transcribed into double-stranded cDNA on magnetic beads covered with oligo d(T), the cDNA was digested with Nla III and ligated to Illumina adapter containing a recognition site of Mme I . .. The mRNAs were isolated and reverse transcribed into double-stranded cDNA on magnetic beads covered with oligo d(T), the cDNA was digested with Nla III and ligated to Illumina adapter containing a recognition site of Mme I .

Nucleic Acid Electrophoresis:

Article Title: Transcriptome differences in the hypopharyngeal gland between Western Honeybees (Apis mellifera) and Eastern Honeybees (Apis cerana)
Article Snippet: The cDNA was digested with Nla III which could recognize CATG site and the Illumina adaptor 1 was ligated to the sticky 5′ end of the digested bead-bound 3′ cDNA fragments. .. The cDNA was digested with Nla III which could recognize CATG site and the Illumina adaptor 1 was ligated to the sticky 5′ end of the digested bead-bound 3′ cDNA fragments.

RNA Sequencing Assay:

Article Title: Genome-Wide Mapping of DNA Methylation in Chicken
Article Snippet: Paragraph title: RNA-Seq ... The mRNAs were isolated and reverse transcribed into double-stranded cDNA on magnetic beads covered with oligo d(T), the cDNA was digested with Nla III and ligated to Illumina adapter containing a recognition site of Mme I .

Magnetic Beads:

Article Title: Transcriptome differences in the hypopharyngeal gland between Western Honeybees (Apis mellifera) and Eastern Honeybees (Apis cerana)
Article Snippet: Briefly, mRNA was purified from 6 μg of total RNA with oligo (dT) magnetic beads and then synthesized into double-stranded DNA (cDNA) by reverse transcription. .. The cDNA was digested with Nla III which could recognize CATG site and the Illumina adaptor 1 was ligated to the sticky 5′ end of the digested bead-bound 3′ cDNA fragments.

Article Title: Transcriptome assembly and expression profiling of molecular responses to cadmium toxicity in hepatopancreas of the freshwater crab Sinopotamon henanense
Article Snippet: In brief, each isolated mRNA sample was respectively enriched by oligo-dT magnetic beads, and prepared for synthesizing double stranded cDNA. .. Subsequently, the bead-bound cDNA was digested with Nla III and added Illumina adapter 1, and then digested with Mme I and added Illumina adapter 2 to create the sequencing library.

Article Title: Genome-Wide Mapping of DNA Methylation in Chicken
Article Snippet: 2 ug total RNA was used in library construction. .. The mRNAs were isolated and reverse transcribed into double-stranded cDNA on magnetic beads covered with oligo d(T), the cDNA was digested with Nla III and ligated to Illumina adapter containing a recognition site of Mme I . .. Following Mme I digestion, a second Illumina adapter was ligated.

Article Title: Histone methylation mediates plasticity of human FOXP3+ regulatory T cells by modulating signature gene expressions
Article Snippet: Briefly, mRNA was first isolated from the total RNA by using Oligo(dT) magnetic beads and then applied as template for cDNA synthesis. .. The resultant cDNA was endonuclease digested ( Nla III), which recognizes and cuts the most 3′ ‘CATG’, and ligated to Illumina adapter 1.

Mutagenesis:

Article Title: Low Temperature Mitigates Cardia Bifida in Zebrafish Embryos
Article Snippet: Total RNA was extracted from 22-ss s1pr2as10 mutant embryos raised at 28.5 or 22.5°C using an RNeasy Plant Mini Kit (Qiagen), and 4 µg was treated with RNase-free DNase I. .. The cDNA was digested with Nla III and ligated to Illumina adaptor 1.

Isolation:

Article Title: Low Temperature Mitigates Cardia Bifida in Zebrafish Embryos
Article Snippet: The cDNA was digested with Nla III and ligated to Illumina adaptor 1. .. PCR was performed using primers GX1 and GX2, which target adaptors 1 and 2, respectively.

Article Title: Transcriptome differences in the hypopharyngeal gland between Western Honeybees (Apis mellifera) and Eastern Honeybees (Apis cerana)
Article Snippet: The total RNA was extracted using the SV Total RNA isolation System (Promega, USA) and then subjected to quality inspection. .. The cDNA was digested with Nla III which could recognize CATG site and the Illumina adaptor 1 was ligated to the sticky 5′ end of the digested bead-bound 3′ cDNA fragments.

Article Title: Transcriptome assembly and expression profiling of molecular responses to cadmium toxicity in hepatopancreas of the freshwater crab Sinopotamon henanense
Article Snippet: In brief, each isolated mRNA sample was respectively enriched by oligo-dT magnetic beads, and prepared for synthesizing double stranded cDNA. .. Subsequently, the bead-bound cDNA was digested with Nla III and added Illumina adapter 1, and then digested with Mme I and added Illumina adapter 2 to create the sequencing library.

Article Title: Genome-Wide Mapping of DNA Methylation in Chicken
Article Snippet: 2 ug total RNA was used in library construction. .. The mRNAs were isolated and reverse transcribed into double-stranded cDNA on magnetic beads covered with oligo d(T), the cDNA was digested with Nla III and ligated to Illumina adapter containing a recognition site of Mme I . .. Following Mme I digestion, a second Illumina adapter was ligated.

Article Title: Histone methylation mediates plasticity of human FOXP3+ regulatory T cells by modulating signature gene expressions
Article Snippet: Briefly, mRNA was first isolated from the total RNA by using Oligo(dT) magnetic beads and then applied as template for cDNA synthesis. .. The resultant cDNA was endonuclease digested ( Nla III), which recognizes and cuts the most 3′ ‘CATG’, and ligated to Illumina adapter 1.

Purification:

Article Title: Low Temperature Mitigates Cardia Bifida in Zebrafish Embryos
Article Snippet: The cDNA was digested with Nla III and ligated to Illumina adaptor 1. .. PCR was performed using primers GX1 and GX2, which target adaptors 1 and 2, respectively.

Article Title: Transcriptome differences in the hypopharyngeal gland between Western Honeybees (Apis mellifera) and Eastern Honeybees (Apis cerana)
Article Snippet: Briefly, mRNA was purified from 6 μg of total RNA with oligo (dT) magnetic beads and then synthesized into double-stranded DNA (cDNA) by reverse transcription. .. The cDNA was digested with Nla III which could recognize CATG site and the Illumina adaptor 1 was ligated to the sticky 5′ end of the digested bead-bound 3′ cDNA fragments.

Article Title: Genome-Wide Mapping of DNA Methylation in Chicken
Article Snippet: The mRNAs were isolated and reverse transcribed into double-stranded cDNA on magnetic beads covered with oligo d(T), the cDNA was digested with Nla III and ligated to Illumina adapter containing a recognition site of Mme I . .. Tags closed to the 3′ terminus of the mRNA were enriched by a 15 cycles PCR.

Sequencing:

Article Title: Low Temperature Mitigates Cardia Bifida in Zebrafish Embryos
Article Snippet: The cDNA was digested with Nla III and ligated to Illumina adaptor 1. .. PCR was performed using primers GX1 and GX2, which target adaptors 1 and 2, respectively.

Article Title: Transcriptome differences in the hypopharyngeal gland between Western Honeybees (Apis mellifera) and Eastern Honeybees (Apis cerana)
Article Snippet: Paragraph title: Digital gene expression library preparation and sequencing ... The cDNA was digested with Nla III which could recognize CATG site and the Illumina adaptor 1 was ligated to the sticky 5′ end of the digested bead-bound 3′ cDNA fragments.

Article Title: Transcriptome assembly and expression profiling of molecular responses to cadmium toxicity in hepatopancreas of the freshwater crab Sinopotamon henanense
Article Snippet: In brief, each isolated mRNA sample was respectively enriched by oligo-dT magnetic beads, and prepared for synthesizing double stranded cDNA. .. Subsequently, the bead-bound cDNA was digested with Nla III and added Illumina adapter 1, and then digested with Mme I and added Illumina adapter 2 to create the sequencing library. .. After amplification by PCR, cDNA tag library was purified and checked.

Article Title: Genome-Wide Mapping of DNA Methylation in Chicken
Article Snippet: The mRNAs were isolated and reverse transcribed into double-stranded cDNA on magnetic beads covered with oligo d(T), the cDNA was digested with Nla III and ligated to Illumina adapter containing a recognition site of Mme I . .. Tags closed to the 3′ terminus of the mRNA were enriched by a 15 cycles PCR.

Article Title: Histone methylation mediates plasticity of human FOXP3+ regulatory T cells by modulating signature gene expressions
Article Snippet: Total RNA was extracted from fixed cells using High pure FFPE RNA Micro Kit (Roche Diagnostic Corp., Indianapolis, IN) and submitted to BGI-Shenzhen (China) for lllumina sequencing. .. The resultant cDNA was endonuclease digested ( Nla III), which recognizes and cuts the most 3′ ‘CATG’, and ligated to Illumina adapter 1.

Polyacrylamide Gel Electrophoresis:

Article Title: Low Temperature Mitigates Cardia Bifida in Zebrafish Embryos
Article Snippet: The cDNA was digested with Nla III and ligated to Illumina adaptor 1. .. PCR was performed using primers GX1 and GX2, which target adaptors 1 and 2, respectively.

Article Title: Transcriptome differences in the hypopharyngeal gland between Western Honeybees (Apis mellifera) and Eastern Honeybees (Apis cerana)
Article Snippet: The cDNA was digested with Nla III which could recognize CATG site and the Illumina adaptor 1 was ligated to the sticky 5′ end of the digested bead-bound 3′ cDNA fragments. .. The cDNA was digested with Nla III which could recognize CATG site and the Illumina adaptor 1 was ligated to the sticky 5′ end of the digested bead-bound 3′ cDNA fragments.

Chromatin Immunoprecipitation:

Article Title: Transcriptome differences in the hypopharyngeal gland between Western Honeybees (Apis mellifera) and Eastern Honeybees (Apis cerana)
Article Snippet: The cDNA was digested with Nla III which could recognize CATG site and the Illumina adaptor 1 was ligated to the sticky 5′ end of the digested bead-bound 3′ cDNA fragments. .. The cDNA tags containing adaptors 1 and 2 were enriched with 15-cycle PCR amplification with the sequencing primers and then purified by 6% PAGE gel electrophoresis.

Article Title: Transcriptome assembly and expression profiling of molecular responses to cadmium toxicity in hepatopancreas of the freshwater crab Sinopotamon henanense
Article Snippet: Subsequently, the bead-bound cDNA was digested with Nla III and added Illumina adapter 1, and then digested with Mme I and added Illumina adapter 2 to create the sequencing library. .. After amplification by PCR, cDNA tag library was purified and checked.

Sample Prep:

Article Title: Transcriptome differences in the hypopharyngeal gland between Western Honeybees (Apis mellifera) and Eastern Honeybees (Apis cerana)
Article Snippet: The tag-seq libraries were then constructed using the Illumina Gene Expression Sample Prep Kit according to the manufacturer’s instruction. .. The cDNA was digested with Nla III which could recognize CATG site and the Illumina adaptor 1 was ligated to the sticky 5′ end of the digested bead-bound 3′ cDNA fragments.

In Situ:

Article Title: Transcriptome assembly and expression profiling of molecular responses to cadmium toxicity in hepatopancreas of the freshwater crab Sinopotamon henanense
Article Snippet: Subsequently, the bead-bound cDNA was digested with Nla III and added Illumina adapter 1, and then digested with Mme I and added Illumina adapter 2 to create the sequencing library. .. After amplification by PCR, cDNA tag library was purified and checked.

Ethanol Precipitation:

Article Title: Genome-Wide Mapping of DNA Methylation in Chicken
Article Snippet: 30 µg total RNA was digested with RNase-Free DNase I (NEB) for 15 min at 37°C, phenol-chloroform extraction and ethanol precipitation to remove DNA contamination. .. The mRNAs were isolated and reverse transcribed into double-stranded cDNA on magnetic beads covered with oligo d(T), the cDNA was digested with Nla III and ligated to Illumina adapter containing a recognition site of Mme I .

Next-Generation Sequencing:

Article Title: Low Temperature Mitigates Cardia Bifida in Zebrafish Embryos
Article Snippet: DGE analysis was performed by BGI through next-generation sequencing (NGS). .. The cDNA was digested with Nla III and ligated to Illumina adaptor 1.

Concentration Assay:

Article Title: Genome-Wide Mapping of DNA Methylation in Chicken
Article Snippet: The concentration and quality of RNA were assessed by Agilent 2100. .. The mRNAs were isolated and reverse transcribed into double-stranded cDNA on magnetic beads covered with oligo d(T), the cDNA was digested with Nla III and ligated to Illumina adapter containing a recognition site of Mme I .

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    Illumina Inc nla iii sample prep kit
    Nla Iii Sample Prep Kit, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 80/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nla iii sample prep kit/product/Illumina Inc
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    Illumina Inc gex nla iii adapter 1
    Gex Nla Iii Adapter 1, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 78/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    gex nla iii adapter 1 - by Bioz Stars, 2019-10
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