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    Structured Review

    Bio-Rad nitrocel memb 0 45 um
    KEY RESOURCES TABLE
    Nitrocel Memb 0 45 Um, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1752 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nitrocel+memb/Nitrocellulose+Membrane%2E+0%2E45+%CE%9Cm/pmc09245451-155-0-4
    Average 96 stars, based on 1752 article reviews
    nitrocel memb 0 45 um - by Bioz Stars, 2026-10
    96/100 stars

    Images

    1) Product Images from "Systematic Characterization of Mutations Altering Protein Degradation in Human Cancers"

    Article Title: Systematic Characterization of Mutations Altering Protein Degradation in Human Cancers

    Journal: Molecular cell

    doi: 10.1016/j.molcel.2021.01.020

    KEY RESOURCES TABLE
    Figure Legend Snippet: KEY RESOURCES TABLE

    Techniques Used: Control, Virus, Recombinant, In Vitro, Transfection, Western Blot, Capsules, Purification, Gel Extraction, cDNA Synthesis, RNA HS Assay, Plasmid Preparation, BIA-KA, Software, Adhesive

    Related Articles

    Software:

    Article Title: Systematic characterization of mutations altering protein degradation in human cancers.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Nuclease-Free Water Ambion Cat#AM9938 Pierce Homobifunctional Cross Linkers Life Technologies Cat#20593 2-Mercaptoethanol Sigma Aldrich Cat#M6250-10ML Dynabeads Protein A Thermo Fisher Scientific Cat#10004D Dynabeads Protein G Thermo Fisher Scientific Cat#10002D EDTA Sigma Aldrich Cat#E8008-100ML Protease/Phosphatase Inhibitor Cocktail (100X) Cell Signaling Technology Cat#5872S Quick-Load 1 kb Plus DNA Ladder New England Biolabs Cat#N0469S LB Broth Mp Biomedicals Cat#244610 L-Broth Agar Large Capsules Mp Biomedicals Cat#MP 113001236 RIPA buffer Invitrogen Cat#R0278 Pierce 16% Formaldehyde (w/v), Methanol-free Life Technologies Cat#28906 Opti-MEM I Reduced Serum Medium, no phenol red Thermo Fisher Scientific Cat#11058021 Cycloheximide powder Cell Signaling Technology Cat#2112 Critical commercial assays QIAprep Spin Miniprep Kit QIAGEN Cat#27106 RNeasy Plus Mini Kit QIAGEN Cat#74134 QIAquick PCR Purification Kit QIAGEN Cat#28104 QIAquick gel extraction kit QIAGEN Cat#28704 Gibson Assembly Master Mix New England Biolabs Cat#E2611L iScript cDNA Synthesis Kit Bio-Rad Laboratories Cat#1708891 SsoAdvanced Univ SYBR Grn Suprmx Bio-Rad Laboratories Cat#1725272 Qubit dsDNA HS Assay Kit Thermo Fisher Scientific Cat#Q32854 Qubit RNA HS Assay Kit Thermo Fisher Scientific Cat#Q32855 GenElute HP Plasmid Maxiprep Kit Sigma-Aldrich Cat#NA0410-1KT Ampure xp Beckman Coulter Cat#A63881 BCA Assay Kit Thermo Fisher Scientific Cat#23225 SMARTer ThruPLEX DNA-Seq Kit Takara Bio Cat#R400675 Experimental models: cell lines Human: HEK293FT Thermo Fisher Scientific Cat#R70007 Human: MDA-MB-231 American Type Culture Collection Cat#ATCC-HTB-26 Human: CAL27 Ravi Uppaluri lab N/A Human: CAL33 Ravi Uppaluri lab N/A Deposited data GATA3 ChIP-Seq This paper GSE162003 Original gel images This paper https://doi.org/10.17632/kgfzbpv2w4.1 Oligonucleotides Primers for PCR, see Table S5 Invitrogen N/A Recombinant DNA hWIP1-FLAG Addgene RRID:Addgene_28105 pHAGE-GATA3 Addgene RRID:Addgene_116747 lentiCRISPR v2 puro Addgene RRID:Addgene_98290 pMD2.G Addgene RRID:Addgene_12259 psPAX2 Addgene RRID:Addgene_12260 pHAGE-CMV-DsRed-IRES-GFP Koren et al., 2018 N/A pHAGE-SFFV-GFP-IRES-DsRed Timms et al., 2019 N/A pLenti-EF1a-PGK-Puro Kai Wucherpfennig lab N/A pLenti-EF1a-GATA3-WT This paper N/A (Continued on next page) e2 Molecular Cell 81, 1292–1308.e1–e11, March 18, 2021 .. REAGENT or RESOURCE SOURCE IDENTIFIER pLenti-EF1a-GATA3-A442M This paper N/A pLenti-EF1a-GATA3-G444E This paper N/A pLenti-EF1a-GATA3-H400 This paper N/A pLenti-EF1a-GATA3-WT-Fg This paper N/A pLenti-EF1a-GATA3-A442M-Fg This paper N/A pLenti-EF1a-GATA3-G444E-Fg This paper N/A pLenti-EF1a-GATA3-H400-Fg This paper N/A pLenti-EF1a-PPM1D-WT This paper N/A pLenti-EF1a-PPM1D-V604Q This paper N/A pLenti-EF1a-PPM1D-C605W This paper N/A pLenti-EF1a-PPM1D-L450 This paper N/A pLenti-EF1a-PPM1D-WT-HA This paper N/A pLenti-EF1a-PPM1D-V604Q-HA This paper N/A pLenti-EF1a-PPM1D-C605W-HA This paper N/A pLenti-EF1a-PPM1D-L450-HA This paper N/A Software and algorithms GraphPad Prism 7 GraphPad Software https://www.graphpad.com DeepDegron This Paper https://github.com/ctokheim/deepDegron Transcription factor inference This Paper https://github.com/ctokheim/tf_association Other Corning Filter System (0.45um) Corning Life Sciences Cat#431096 milliTUBE 1 ml AFA Fiber Covaris Inc. Cat520130 NITROCEL MEMB 0.45um Bio-Rad Laboratories Cat#1620115 Multiplate 96-Well PCR Plates Bio-Rad Laboratories Cat#MLL9601 QUBIT ASSAY TUBES SET Life Technologies Cat#Q32856 Microseal ‘B’ Adhesive Seals Bio-Rad Laboratories Cat#MSB-1001 ..

    Polymerase Chain Reaction:

    Article Title: Systematic characterization of mutations altering protein degradation in human cancers.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Nuclease-Free Water Ambion Cat#AM9938 Pierce Homobifunctional Cross Linkers Life Technologies Cat#20593 2-Mercaptoethanol Sigma Aldrich Cat#M6250-10ML Dynabeads Protein A Thermo Fisher Scientific Cat#10004D Dynabeads Protein G Thermo Fisher Scientific Cat#10002D EDTA Sigma Aldrich Cat#E8008-100ML Protease/Phosphatase Inhibitor Cocktail (100X) Cell Signaling Technology Cat#5872S Quick-Load 1 kb Plus DNA Ladder New England Biolabs Cat#N0469S LB Broth Mp Biomedicals Cat#244610 L-Broth Agar Large Capsules Mp Biomedicals Cat#MP 113001236 RIPA buffer Invitrogen Cat#R0278 Pierce 16% Formaldehyde (w/v), Methanol-free Life Technologies Cat#28906 Opti-MEM I Reduced Serum Medium, no phenol red Thermo Fisher Scientific Cat#11058021 Cycloheximide powder Cell Signaling Technology Cat#2112 Critical commercial assays QIAprep Spin Miniprep Kit QIAGEN Cat#27106 RNeasy Plus Mini Kit QIAGEN Cat#74134 QIAquick PCR Purification Kit QIAGEN Cat#28104 QIAquick gel extraction kit QIAGEN Cat#28704 Gibson Assembly Master Mix New England Biolabs Cat#E2611L iScript cDNA Synthesis Kit Bio-Rad Laboratories Cat#1708891 SsoAdvanced Univ SYBR Grn Suprmx Bio-Rad Laboratories Cat#1725272 Qubit dsDNA HS Assay Kit Thermo Fisher Scientific Cat#Q32854 Qubit RNA HS Assay Kit Thermo Fisher Scientific Cat#Q32855 GenElute HP Plasmid Maxiprep Kit Sigma-Aldrich Cat#NA0410-1KT Ampure xp Beckman Coulter Cat#A63881 BCA Assay Kit Thermo Fisher Scientific Cat#23225 SMARTer ThruPLEX DNA-Seq Kit Takara Bio Cat#R400675 Experimental models: cell lines Human: HEK293FT Thermo Fisher Scientific Cat#R70007 Human: MDA-MB-231 American Type Culture Collection Cat#ATCC-HTB-26 Human: CAL27 Ravi Uppaluri lab N/A Human: CAL33 Ravi Uppaluri lab N/A Deposited data GATA3 ChIP-Seq This paper GSE162003 Original gel images This paper https://doi.org/10.17632/kgfzbpv2w4.1 Oligonucleotides Primers for PCR, see Table S5 Invitrogen N/A Recombinant DNA hWIP1-FLAG Addgene RRID:Addgene_28105 pHAGE-GATA3 Addgene RRID:Addgene_116747 lentiCRISPR v2 puro Addgene RRID:Addgene_98290 pMD2.G Addgene RRID:Addgene_12259 psPAX2 Addgene RRID:Addgene_12260 pHAGE-CMV-DsRed-IRES-GFP Koren et al., 2018 N/A pHAGE-SFFV-GFP-IRES-DsRed Timms et al., 2019 N/A pLenti-EF1a-PGK-Puro Kai Wucherpfennig lab N/A pLenti-EF1a-GATA3-WT This paper N/A (Continued on next page) e2 Molecular Cell 81, 1292–1308.e1–e11, March 18, 2021 .. REAGENT or RESOURCE SOURCE IDENTIFIER pLenti-EF1a-GATA3-A442M This paper N/A pLenti-EF1a-GATA3-G444E This paper N/A pLenti-EF1a-GATA3-H400 This paper N/A pLenti-EF1a-GATA3-WT-Fg This paper N/A pLenti-EF1a-GATA3-A442M-Fg This paper N/A pLenti-EF1a-GATA3-G444E-Fg This paper N/A pLenti-EF1a-GATA3-H400-Fg This paper N/A pLenti-EF1a-PPM1D-WT This paper N/A pLenti-EF1a-PPM1D-V604Q This paper N/A pLenti-EF1a-PPM1D-C605W This paper N/A pLenti-EF1a-PPM1D-L450 This paper N/A pLenti-EF1a-PPM1D-WT-HA This paper N/A pLenti-EF1a-PPM1D-V604Q-HA This paper N/A pLenti-EF1a-PPM1D-C605W-HA This paper N/A pLenti-EF1a-PPM1D-L450-HA This paper N/A Software and algorithms GraphPad Prism 7 GraphPad Software https://www.graphpad.com DeepDegron This Paper https://github.com/ctokheim/deepDegron Transcription factor inference This Paper https://github.com/ctokheim/tf_association Other Corning Filter System (0.45um) Corning Life Sciences Cat#431096 milliTUBE 1 ml AFA Fiber Covaris Inc. Cat520130 NITROCEL MEMB 0.45um Bio-Rad Laboratories Cat#1620115 Multiplate 96-Well PCR Plates Bio-Rad Laboratories Cat#MLL9601 QUBIT ASSAY TUBES SET Life Technologies Cat#Q32856 Microseal ‘B’ Adhesive Seals Bio-Rad Laboratories Cat#MSB-1001 ..

    Article Title: In vivo CRISPR screens identify the E3 ligase Cop1 as a modulator of macrophage infiltration and cancer immunotherapy target.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Gibson Assembly Master Mix New England Biolabs Cat# E2611L iScript cDNA Synthesis Kit Bio-Rad Laboratories Cat# 1708891 SsoAdvanced Univ SYBR Grn Suprmx Bio-Rad Laboratories Cat# 1725272 Qubit dsDNA HS Assay Kit Thermo Fisher Scientific Cat# Q32854 Qubit RNA HS Assay Kit Thermo Fisher Scientific Cat# Q32855 GenElute HP Plasmid Maxiprep Kit Sigma-Aldrich Cat# NA0410-1KT Ampure xp Beckman Coulter Cat# A63881 Mouse Cytokine Array RayBiotech Cat# C1000 BCA Assay Kit Thermo Fisher Scientific Cat# 23225 SMARTer ThruPLEX DNA-Seq Kit Takara Bio Cat# R400675 Oligonucleotides MusCK oligo pool Twist bioscience N/A MusCK2.0 oligo pool Twist bioscience N/A Primers for knockout or real-time PCR, see Data and code availability This paper N/A Recombinant DNA lentiCRISPR v2 blast Addgene RRID:Addgene_83480 lentiCRISPR v2 puro Addgene RRID:Addgene_98290 pMD2.G Addgene RRID:Addgene_12259 psPAX2 Addgene RRID:Addgene_12260 pCI-neo-sOVA Addgene RRID:Addgene_25098 pCI-neo-mOVA Addgene RRID:Addgene_25099 pCI-neo-cOVA Addgene RRID:Addgene_25097 pcDNA3-OVA Addgene RRID:Addgene_64599 lentiV2-blast-sOva This paper N/A lentiV2-blast-mOva This paper N/A lentiV2-blast-cOva This paper N/A lentiV2-blast-Ova This paper N/A pLentiCRISPR-EGFP Addgene RRID:Addgene_75159 pEF1A-puro This paper N/A pLentiCRISPR-mCherry Addgene RRID:Addgene_75161 Software and algorithms GraphPad Prism 7 GraphPad Software https://www.graphpad.com GSEA Mootha et al., 2003 https://www.gsea-msigdb.org/ gsea/msigdb/annotate.jsp Flow Jo_v10 FlowJo https://www.flowjo.com/ BWA Li and Durbin, 2009 https://github.com/lh3/bwa CoolBox Xu et al., 2021 https://github.com/ GangCaoLab/CoolBox Bowtie2 Langmead and Salzberg, 2012 http://bowtie-bio.sourceforge. net/bowtie2/index.shtml LISA Qin et al., 2020 http://cistrome.org Cistrome-GO Li et al., 2019 http://go.cistrome.org LIMMA Ritchie et al., 2015 https://bioconductor.org/packages/ release/bioc/html/limma.html Samtools Li et al., 2009 http://samtools.sourceforge.net/ Deposited data Data files for RNA-seq (processed data) This paper The NCBI GEO database (GSE171467, GSE173296) (Continued on next page) ll Cell 184, 5357–5374.e1–e10, October 14, 2021 e3 Article .. REAGENT or RESOURCE SOURCE IDENTIFIER Data files for ATAC-seq (processed data) This paper The NCBI GEO database (GSE174784) Data files for ChIP-seq (processed data) This paper The NCBI GEO database (GSE175332) Primer sequence This paper https://data.mendeley.com/ datasets/9d5499gb8x/1 Code This paper https://github.com/liulab- dfci/Cop1_Cell_2021 Other Corning Filter System (0.45um) Corning Life Sciences Cat# 431096 milliTUBE 1 ml AFA Fiber Covaris Inc. Cat# 520130 NITROCEL MEMB 0.45um Bio-Rad Laboratories Cat# 1620115 Multiplate 96-Well PCR Plates Bio-Rad Laboratories Cat# MLL9601 QUBIT ASSAY TUBES SET Life Technologies Cat# Q32856 Microseal B Adhesive Seals Bio-Rad Laboratories Cat# MSB-1001 ll Article ..

    Adhesive:

    Article Title: Systematic characterization of mutations altering protein degradation in human cancers.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Nuclease-Free Water Ambion Cat#AM9938 Pierce Homobifunctional Cross Linkers Life Technologies Cat#20593 2-Mercaptoethanol Sigma Aldrich Cat#M6250-10ML Dynabeads Protein A Thermo Fisher Scientific Cat#10004D Dynabeads Protein G Thermo Fisher Scientific Cat#10002D EDTA Sigma Aldrich Cat#E8008-100ML Protease/Phosphatase Inhibitor Cocktail (100X) Cell Signaling Technology Cat#5872S Quick-Load 1 kb Plus DNA Ladder New England Biolabs Cat#N0469S LB Broth Mp Biomedicals Cat#244610 L-Broth Agar Large Capsules Mp Biomedicals Cat#MP 113001236 RIPA buffer Invitrogen Cat#R0278 Pierce 16% Formaldehyde (w/v), Methanol-free Life Technologies Cat#28906 Opti-MEM I Reduced Serum Medium, no phenol red Thermo Fisher Scientific Cat#11058021 Cycloheximide powder Cell Signaling Technology Cat#2112 Critical commercial assays QIAprep Spin Miniprep Kit QIAGEN Cat#27106 RNeasy Plus Mini Kit QIAGEN Cat#74134 QIAquick PCR Purification Kit QIAGEN Cat#28104 QIAquick gel extraction kit QIAGEN Cat#28704 Gibson Assembly Master Mix New England Biolabs Cat#E2611L iScript cDNA Synthesis Kit Bio-Rad Laboratories Cat#1708891 SsoAdvanced Univ SYBR Grn Suprmx Bio-Rad Laboratories Cat#1725272 Qubit dsDNA HS Assay Kit Thermo Fisher Scientific Cat#Q32854 Qubit RNA HS Assay Kit Thermo Fisher Scientific Cat#Q32855 GenElute HP Plasmid Maxiprep Kit Sigma-Aldrich Cat#NA0410-1KT Ampure xp Beckman Coulter Cat#A63881 BCA Assay Kit Thermo Fisher Scientific Cat#23225 SMARTer ThruPLEX DNA-Seq Kit Takara Bio Cat#R400675 Experimental models: cell lines Human: HEK293FT Thermo Fisher Scientific Cat#R70007 Human: MDA-MB-231 American Type Culture Collection Cat#ATCC-HTB-26 Human: CAL27 Ravi Uppaluri lab N/A Human: CAL33 Ravi Uppaluri lab N/A Deposited data GATA3 ChIP-Seq This paper GSE162003 Original gel images This paper https://doi.org/10.17632/kgfzbpv2w4.1 Oligonucleotides Primers for PCR, see Table S5 Invitrogen N/A Recombinant DNA hWIP1-FLAG Addgene RRID:Addgene_28105 pHAGE-GATA3 Addgene RRID:Addgene_116747 lentiCRISPR v2 puro Addgene RRID:Addgene_98290 pMD2.G Addgene RRID:Addgene_12259 psPAX2 Addgene RRID:Addgene_12260 pHAGE-CMV-DsRed-IRES-GFP Koren et al., 2018 N/A pHAGE-SFFV-GFP-IRES-DsRed Timms et al., 2019 N/A pLenti-EF1a-PGK-Puro Kai Wucherpfennig lab N/A pLenti-EF1a-GATA3-WT This paper N/A (Continued on next page) e2 Molecular Cell 81, 1292–1308.e1–e11, March 18, 2021 .. REAGENT or RESOURCE SOURCE IDENTIFIER pLenti-EF1a-GATA3-A442M This paper N/A pLenti-EF1a-GATA3-G444E This paper N/A pLenti-EF1a-GATA3-H400 This paper N/A pLenti-EF1a-GATA3-WT-Fg This paper N/A pLenti-EF1a-GATA3-A442M-Fg This paper N/A pLenti-EF1a-GATA3-G444E-Fg This paper N/A pLenti-EF1a-GATA3-H400-Fg This paper N/A pLenti-EF1a-PPM1D-WT This paper N/A pLenti-EF1a-PPM1D-V604Q This paper N/A pLenti-EF1a-PPM1D-C605W This paper N/A pLenti-EF1a-PPM1D-L450 This paper N/A pLenti-EF1a-PPM1D-WT-HA This paper N/A pLenti-EF1a-PPM1D-V604Q-HA This paper N/A pLenti-EF1a-PPM1D-C605W-HA This paper N/A pLenti-EF1a-PPM1D-L450-HA This paper N/A Software and algorithms GraphPad Prism 7 GraphPad Software https://www.graphpad.com DeepDegron This Paper https://github.com/ctokheim/deepDegron Transcription factor inference This Paper https://github.com/ctokheim/tf_association Other Corning Filter System (0.45um) Corning Life Sciences Cat#431096 milliTUBE 1 ml AFA Fiber Covaris Inc. Cat520130 NITROCEL MEMB 0.45um Bio-Rad Laboratories Cat#1620115 Multiplate 96-Well PCR Plates Bio-Rad Laboratories Cat#MLL9601 QUBIT ASSAY TUBES SET Life Technologies Cat#Q32856 Microseal ‘B’ Adhesive Seals Bio-Rad Laboratories Cat#MSB-1001 ..

    Article Title: In vivo CRISPR screens identify the E3 ligase Cop1 as a modulator of macrophage infiltration and cancer immunotherapy target.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Gibson Assembly Master Mix New England Biolabs Cat# E2611L iScript cDNA Synthesis Kit Bio-Rad Laboratories Cat# 1708891 SsoAdvanced Univ SYBR Grn Suprmx Bio-Rad Laboratories Cat# 1725272 Qubit dsDNA HS Assay Kit Thermo Fisher Scientific Cat# Q32854 Qubit RNA HS Assay Kit Thermo Fisher Scientific Cat# Q32855 GenElute HP Plasmid Maxiprep Kit Sigma-Aldrich Cat# NA0410-1KT Ampure xp Beckman Coulter Cat# A63881 Mouse Cytokine Array RayBiotech Cat# C1000 BCA Assay Kit Thermo Fisher Scientific Cat# 23225 SMARTer ThruPLEX DNA-Seq Kit Takara Bio Cat# R400675 Oligonucleotides MusCK oligo pool Twist bioscience N/A MusCK2.0 oligo pool Twist bioscience N/A Primers for knockout or real-time PCR, see Data and code availability This paper N/A Recombinant DNA lentiCRISPR v2 blast Addgene RRID:Addgene_83480 lentiCRISPR v2 puro Addgene RRID:Addgene_98290 pMD2.G Addgene RRID:Addgene_12259 psPAX2 Addgene RRID:Addgene_12260 pCI-neo-sOVA Addgene RRID:Addgene_25098 pCI-neo-mOVA Addgene RRID:Addgene_25099 pCI-neo-cOVA Addgene RRID:Addgene_25097 pcDNA3-OVA Addgene RRID:Addgene_64599 lentiV2-blast-sOva This paper N/A lentiV2-blast-mOva This paper N/A lentiV2-blast-cOva This paper N/A lentiV2-blast-Ova This paper N/A pLentiCRISPR-EGFP Addgene RRID:Addgene_75159 pEF1A-puro This paper N/A pLentiCRISPR-mCherry Addgene RRID:Addgene_75161 Software and algorithms GraphPad Prism 7 GraphPad Software https://www.graphpad.com GSEA Mootha et al., 2003 https://www.gsea-msigdb.org/ gsea/msigdb/annotate.jsp Flow Jo_v10 FlowJo https://www.flowjo.com/ BWA Li and Durbin, 2009 https://github.com/lh3/bwa CoolBox Xu et al., 2021 https://github.com/ GangCaoLab/CoolBox Bowtie2 Langmead and Salzberg, 2012 http://bowtie-bio.sourceforge. net/bowtie2/index.shtml LISA Qin et al., 2020 http://cistrome.org Cistrome-GO Li et al., 2019 http://go.cistrome.org LIMMA Ritchie et al., 2015 https://bioconductor.org/packages/ release/bioc/html/limma.html Samtools Li et al., 2009 http://samtools.sourceforge.net/ Deposited data Data files for RNA-seq (processed data) This paper The NCBI GEO database (GSE171467, GSE173296) (Continued on next page) ll Cell 184, 5357–5374.e1–e10, October 14, 2021 e3 Article .. REAGENT or RESOURCE SOURCE IDENTIFIER Data files for ATAC-seq (processed data) This paper The NCBI GEO database (GSE174784) Data files for ChIP-seq (processed data) This paper The NCBI GEO database (GSE175332) Primer sequence This paper https://data.mendeley.com/ datasets/9d5499gb8x/1 Code This paper https://github.com/liulab- dfci/Cop1_Cell_2021 Other Corning Filter System (0.45um) Corning Life Sciences Cat# 431096 milliTUBE 1 ml AFA Fiber Covaris Inc. Cat# 520130 NITROCEL MEMB 0.45um Bio-Rad Laboratories Cat# 1620115 Multiplate 96-Well PCR Plates Bio-Rad Laboratories Cat# MLL9601 QUBIT ASSAY TUBES SET Life Technologies Cat# Q32856 Microseal B Adhesive Seals Bio-Rad Laboratories Cat# MSB-1001 ll Article ..

    Chromatin Immunoprecipitation:

    Article Title: In vivo CRISPR screens identify the E3 ligase Cop1 as a modulator of macrophage infiltration and cancer immunotherapy target.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Gibson Assembly Master Mix New England Biolabs Cat# E2611L iScript cDNA Synthesis Kit Bio-Rad Laboratories Cat# 1708891 SsoAdvanced Univ SYBR Grn Suprmx Bio-Rad Laboratories Cat# 1725272 Qubit dsDNA HS Assay Kit Thermo Fisher Scientific Cat# Q32854 Qubit RNA HS Assay Kit Thermo Fisher Scientific Cat# Q32855 GenElute HP Plasmid Maxiprep Kit Sigma-Aldrich Cat# NA0410-1KT Ampure xp Beckman Coulter Cat# A63881 Mouse Cytokine Array RayBiotech Cat# C1000 BCA Assay Kit Thermo Fisher Scientific Cat# 23225 SMARTer ThruPLEX DNA-Seq Kit Takara Bio Cat# R400675 Oligonucleotides MusCK oligo pool Twist bioscience N/A MusCK2.0 oligo pool Twist bioscience N/A Primers for knockout or real-time PCR, see Data and code availability This paper N/A Recombinant DNA lentiCRISPR v2 blast Addgene RRID:Addgene_83480 lentiCRISPR v2 puro Addgene RRID:Addgene_98290 pMD2.G Addgene RRID:Addgene_12259 psPAX2 Addgene RRID:Addgene_12260 pCI-neo-sOVA Addgene RRID:Addgene_25098 pCI-neo-mOVA Addgene RRID:Addgene_25099 pCI-neo-cOVA Addgene RRID:Addgene_25097 pcDNA3-OVA Addgene RRID:Addgene_64599 lentiV2-blast-sOva This paper N/A lentiV2-blast-mOva This paper N/A lentiV2-blast-cOva This paper N/A lentiV2-blast-Ova This paper N/A pLentiCRISPR-EGFP Addgene RRID:Addgene_75159 pEF1A-puro This paper N/A pLentiCRISPR-mCherry Addgene RRID:Addgene_75161 Software and algorithms GraphPad Prism 7 GraphPad Software https://www.graphpad.com GSEA Mootha et al., 2003 https://www.gsea-msigdb.org/ gsea/msigdb/annotate.jsp Flow Jo_v10 FlowJo https://www.flowjo.com/ BWA Li and Durbin, 2009 https://github.com/lh3/bwa CoolBox Xu et al., 2021 https://github.com/ GangCaoLab/CoolBox Bowtie2 Langmead and Salzberg, 2012 http://bowtie-bio.sourceforge. net/bowtie2/index.shtml LISA Qin et al., 2020 http://cistrome.org Cistrome-GO Li et al., 2019 http://go.cistrome.org LIMMA Ritchie et al., 2015 https://bioconductor.org/packages/ release/bioc/html/limma.html Samtools Li et al., 2009 http://samtools.sourceforge.net/ Deposited data Data files for RNA-seq (processed data) This paper The NCBI GEO database (GSE171467, GSE173296) (Continued on next page) ll Cell 184, 5357–5374.e1–e10, October 14, 2021 e3 Article .. REAGENT or RESOURCE SOURCE IDENTIFIER Data files for ATAC-seq (processed data) This paper The NCBI GEO database (GSE174784) Data files for ChIP-seq (processed data) This paper The NCBI GEO database (GSE175332) Primer sequence This paper https://data.mendeley.com/ datasets/9d5499gb8x/1 Code This paper https://github.com/liulab- dfci/Cop1_Cell_2021 Other Corning Filter System (0.45um) Corning Life Sciences Cat# 431096 milliTUBE 1 ml AFA Fiber Covaris Inc. Cat# 520130 NITROCEL MEMB 0.45um Bio-Rad Laboratories Cat# 1620115 Multiplate 96-Well PCR Plates Bio-Rad Laboratories Cat# MLL9601 QUBIT ASSAY TUBES SET Life Technologies Cat# Q32856 Microseal B Adhesive Seals Bio-Rad Laboratories Cat# MSB-1001 ll Article ..

    Sequencing:

    Article Title: In vivo CRISPR screens identify the E3 ligase Cop1 as a modulator of macrophage infiltration and cancer immunotherapy target.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Gibson Assembly Master Mix New England Biolabs Cat# E2611L iScript cDNA Synthesis Kit Bio-Rad Laboratories Cat# 1708891 SsoAdvanced Univ SYBR Grn Suprmx Bio-Rad Laboratories Cat# 1725272 Qubit dsDNA HS Assay Kit Thermo Fisher Scientific Cat# Q32854 Qubit RNA HS Assay Kit Thermo Fisher Scientific Cat# Q32855 GenElute HP Plasmid Maxiprep Kit Sigma-Aldrich Cat# NA0410-1KT Ampure xp Beckman Coulter Cat# A63881 Mouse Cytokine Array RayBiotech Cat# C1000 BCA Assay Kit Thermo Fisher Scientific Cat# 23225 SMARTer ThruPLEX DNA-Seq Kit Takara Bio Cat# R400675 Oligonucleotides MusCK oligo pool Twist bioscience N/A MusCK2.0 oligo pool Twist bioscience N/A Primers for knockout or real-time PCR, see Data and code availability This paper N/A Recombinant DNA lentiCRISPR v2 blast Addgene RRID:Addgene_83480 lentiCRISPR v2 puro Addgene RRID:Addgene_98290 pMD2.G Addgene RRID:Addgene_12259 psPAX2 Addgene RRID:Addgene_12260 pCI-neo-sOVA Addgene RRID:Addgene_25098 pCI-neo-mOVA Addgene RRID:Addgene_25099 pCI-neo-cOVA Addgene RRID:Addgene_25097 pcDNA3-OVA Addgene RRID:Addgene_64599 lentiV2-blast-sOva This paper N/A lentiV2-blast-mOva This paper N/A lentiV2-blast-cOva This paper N/A lentiV2-blast-Ova This paper N/A pLentiCRISPR-EGFP Addgene RRID:Addgene_75159 pEF1A-puro This paper N/A pLentiCRISPR-mCherry Addgene RRID:Addgene_75161 Software and algorithms GraphPad Prism 7 GraphPad Software https://www.graphpad.com GSEA Mootha et al., 2003 https://www.gsea-msigdb.org/ gsea/msigdb/annotate.jsp Flow Jo_v10 FlowJo https://www.flowjo.com/ BWA Li and Durbin, 2009 https://github.com/lh3/bwa CoolBox Xu et al., 2021 https://github.com/ GangCaoLab/CoolBox Bowtie2 Langmead and Salzberg, 2012 http://bowtie-bio.sourceforge. net/bowtie2/index.shtml LISA Qin et al., 2020 http://cistrome.org Cistrome-GO Li et al., 2019 http://go.cistrome.org LIMMA Ritchie et al., 2015 https://bioconductor.org/packages/ release/bioc/html/limma.html Samtools Li et al., 2009 http://samtools.sourceforge.net/ Deposited data Data files for RNA-seq (processed data) This paper The NCBI GEO database (GSE171467, GSE173296) (Continued on next page) ll Cell 184, 5357–5374.e1–e10, October 14, 2021 e3 Article .. REAGENT or RESOURCE SOURCE IDENTIFIER Data files for ATAC-seq (processed data) This paper The NCBI GEO database (GSE174784) Data files for ChIP-seq (processed data) This paper The NCBI GEO database (GSE175332) Primer sequence This paper https://data.mendeley.com/ datasets/9d5499gb8x/1 Code This paper https://github.com/liulab- dfci/Cop1_Cell_2021 Other Corning Filter System (0.45um) Corning Life Sciences Cat# 431096 milliTUBE 1 ml AFA Fiber Covaris Inc. Cat# 520130 NITROCEL MEMB 0.45um Bio-Rad Laboratories Cat# 1620115 Multiplate 96-Well PCR Plates Bio-Rad Laboratories Cat# MLL9601 QUBIT ASSAY TUBES SET Life Technologies Cat# Q32856 Microseal B Adhesive Seals Bio-Rad Laboratories Cat# MSB-1001 ll Article ..



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    KEY RESOURCES TABLE

    Journal: Molecular cell

    Article Title: Systematic Characterization of Mutations Altering Protein Degradation in Human Cancers

    doi: 10.1016/j.molcel.2021.01.020

    Figure Lengend Snippet: KEY RESOURCES TABLE

    Article Snippet: NITROCEL MEMB 0.45um , Bio-Rad Laboratories , Cat#1620115.

    Techniques: Control, Virus, Recombinant, In Vitro, Transfection, Western Blot, Capsules, Purification, Gel Extraction, cDNA Synthesis, RNA HS Assay, Plasmid Preparation, BIA-KA, Software, Adhesive