nitrocefine disks  (Thermo Fisher)


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    Name:
    Oxoid Oxoid Salmonella Latex Test
    Description:
    Following the discontinuation of the Thermo Scientific Oxoid Salmonella Rapid Test FT0201A the decision has been made to discontinue the Thermo Scientific Oxoid Salmonella Latex Test FT0203A which was introduced as part of the OSRT range There is an alternative kit available The Thermo Scientific Oxoid Salmonella Test Kit DR1108A is manufactured using the same antibody as the Oxoid Salmonella Latex Test FT0203A A positive control has now been added to the DR1108A kit
    Catalog Number:
    ft0203a
    Price:
    None
    Applications:
    Clinical|Clinical Microbiology
    Category:
    Kits and Assays
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    Structured Review

    Thermo Fisher nitrocefine disks
    Following the discontinuation of the Thermo Scientific Oxoid Salmonella Rapid Test FT0201A the decision has been made to discontinue the Thermo Scientific Oxoid Salmonella Latex Test FT0203A which was introduced as part of the OSRT range There is an alternative kit available The Thermo Scientific Oxoid Salmonella Test Kit DR1108A is manufactured using the same antibody as the Oxoid Salmonella Latex Test FT0203A A positive control has now been added to the DR1108A kit
    https://www.bioz.com/result/nitrocefine disks/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    nitrocefine disks - by Bioz Stars, 2020-09
    99/100 stars

    Related Products / Commonly Used Together

    β-lactamase production

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    Related Articles

    Diagnostic Assay:

    Article Title: Prevalence of Group A Streptococcal Carriers in Asymptomatic Children and Clonal Relatedness among Isolates in Malatya, Turkey
    Article Snippet: .. After incubation overnight at 37°C, beta-hemolytic streptococci were identified with a bacitracin disk (0.04 U) and a latex test for the identification of streptococcal groups A, B, C, D, F, and G (streptococcal grouping kit and diagnostic reagent; Oxoid Limited, Basingstoke, England). .. The antimicrobial susceptibilities of the GAS isolates were investigated by the disk diffusion method according to the criteria of the National Committee for Clinical Laboratory Standards ( ).

    Polymerase Chain Reaction:

    Article Title: Socially engaged calves are more likely to be colonised by VTEC O157:H7 than individuals showing signs of poor welfare
    Article Snippet: .. Up to 5 suspected colonies were picked for agglutination with a latex kit (DR 622; Oxoid) and if positive for VTEC O157:H7 genes encoding O157 (rfbO157 ), verotoxin 1 (vtx1) and verotoxin 2 (vtx2) as well as intimin (eaeA) were established by PCR , . .. Analysis of isolates by real-time PCR (as described by Söderlund et al . ) established that 11 of the farms had the highly virulent type clade 8.

    Incubation:

    Article Title: Prevalence of Group A Streptococcal Carriers in Asymptomatic Children and Clonal Relatedness among Isolates in Malatya, Turkey
    Article Snippet: .. After incubation overnight at 37°C, beta-hemolytic streptococci were identified with a bacitracin disk (0.04 U) and a latex test for the identification of streptococcal groups A, B, C, D, F, and G (streptococcal grouping kit and diagnostic reagent; Oxoid Limited, Basingstoke, England). .. The antimicrobial susceptibilities of the GAS isolates were investigated by the disk diffusion method according to the criteria of the National Committee for Clinical Laboratory Standards ( ).

    Article Title: Efficacy of Electrolyzed Oxidizing Water for Inactivating Escherichia coli O157:H7, Salmonella enteritidis, and Listeria monocytogenes
    Article Snippet: .. Colonies of the inoculated pathogen were enumerated on TSA plates after incubation at 37°C for 48 h. A volume of 1 ml of the inoculated solution (treatment or control) after exposure to each temperature-time combination was also transferred to separate 250-ml Erlenmeyer flasks containing 100 ml of sterile TSB and incubated at 37°C for 24 h. Following enrichment in TSB, the culture was streaked on either sorbitol MacConkey agar no. 3 (Oxoid Division, Unipath Co., Ogdensburg, N.Y.) (for E. coli O157:H7), xylose lysine deoxycholate agar (Gene-Trak, Framingham, Mass.) (for S. enteritidis ), or Oxford agar (Gene-Trak) (for L. monocytogenes ), and the plates were incubated at 37°C for 24 h. Representative colonies of E. coli O157:H7 and S. enteritidis from the respective plates were confirmed by the E. coli O157:H7 latex agglutination assay (Remel Microbiology Products, Lenexa, Kans.) and the Salmonella latex test (Oxoid), respectively. .. The colonies of L. monocytogenes on Oxford agar were confirmed by the API-20E diagnostic test kit (Biomerieux, Hazelwood, Mo.).

    Article Title: Salmonella Typhimurium and Salmonella Sofia: Growth in and Persistence on Eggs under Production and Retail Conditions
    Article Snippet: .. Enrichments were streaked onto TSA and incubated at 37°C for 24 ± 2 h. After counting, three colonies were selected from the TSA plates of each isolate at the beginning and end of each trial and confirmed as Salmonella using a Salmonella latex test kit (Oxoid). ..

    Agglutination:

    Article Title: Socially engaged calves are more likely to be colonised by VTEC O157:H7 than individuals showing signs of poor welfare
    Article Snippet: .. Up to 5 suspected colonies were picked for agglutination with a latex kit (DR 622; Oxoid) and if positive for VTEC O157:H7 genes encoding O157 (rfbO157 ), verotoxin 1 (vtx1) and verotoxin 2 (vtx2) as well as intimin (eaeA) were established by PCR , . .. Analysis of isolates by real-time PCR (as described by Söderlund et al . ) established that 11 of the farms had the highly virulent type clade 8.

    Article Title: Efficacy of Electrolyzed Oxidizing Water for Inactivating Escherichia coli O157:H7, Salmonella enteritidis, and Listeria monocytogenes
    Article Snippet: .. Colonies of the inoculated pathogen were enumerated on TSA plates after incubation at 37°C for 48 h. A volume of 1 ml of the inoculated solution (treatment or control) after exposure to each temperature-time combination was also transferred to separate 250-ml Erlenmeyer flasks containing 100 ml of sterile TSB and incubated at 37°C for 24 h. Following enrichment in TSB, the culture was streaked on either sorbitol MacConkey agar no. 3 (Oxoid Division, Unipath Co., Ogdensburg, N.Y.) (for E. coli O157:H7), xylose lysine deoxycholate agar (Gene-Trak, Framingham, Mass.) (for S. enteritidis ), or Oxford agar (Gene-Trak) (for L. monocytogenes ), and the plates were incubated at 37°C for 24 h. Representative colonies of E. coli O157:H7 and S. enteritidis from the respective plates were confirmed by the E. coli O157:H7 latex agglutination assay (Remel Microbiology Products, Lenexa, Kans.) and the Salmonella latex test (Oxoid), respectively. .. The colonies of L. monocytogenes on Oxford agar were confirmed by the API-20E diagnostic test kit (Biomerieux, Hazelwood, Mo.).

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  • 90
    Thermo Fisher nitrocefin
    Kinetics of changes in E. coli ML-35p outer and cytoplasmic membrane permeability measured with the use of chromogenic markers – the products of <t>nitrocefin</t> (OD 540 ) and ONPG (OD 405 ) hydrolysis, respectively. Outer membrane permeability resulting from incubation of bacteria with mini-ChBac7.5Nα at various concentrations (from 0.125 to 32 μM, highlighted with colors) in the absence (A) or in the presence (B) of 0.9% NaCl. Melittin and ChMAP-28 at concentration of 1 μM were used as positive control samples. (C) Analysis of outer membrane permeability resulting from incubation with ChMAP-28 or melittin in the presence of 0.9% NaCl. (D) Comparative analysis of cytoplasmic membrane permeability resulting from incubation with the individual ChMAP-28 or with its combinations with mini-ChBac7.5Nα. Melittin at concentration of 8 μM was used as a positive control. Three independent experiments were performed, and the curve pattern was similar for the three series.
    Nitrocefin, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nitrocefin/product/Thermo Fisher
    Average 90 stars, based on 25 article reviews
    Price from $9.99 to $1999.99
    nitrocefin - by Bioz Stars, 2020-09
    90/100 stars
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    94
    carbosynth nitrocefin
    Biochemical analysis of relebactam inhibition of M. abscessus β -lactamase, Bla Mab . Our novel Thin Layer Chromatography (TLC) assay (2a) exhibiting the activity of Bla Mab in the turnover of penicillin V (high R f value) to penicilloic acid (lower R f value). In the absence, or termination of activity of Bla Mab (by boiling (100 °C) for 1 h) or addition of known inhibitor avibactam ( Lefebvre et al., 2017 ) (200 μg/mL) no lower R f value spot corresponding to penicilloic acid is seen on the TLC plate. The addition of relebactam to the reaction between Bla Mab and penicillin V also results in the absence of the lower Rf value spot, suggesting inhibition of Bla Mab (2a). This observed inhibition is validated by a spectrophotometric analysis, where the turnover of <t>nitrocefin</t> (100 μM) by Bla Mab (0.25 nM) was monitored at 486 nm with a varying concentration of relebactam (0, 0.1, 1, 10 and 100 μM). The increase in concentration of relebactam resulted in partial inhibition of nitrocefin turnover at 1 μM and total abrogation at 10 μM (2b). The initial velocity (v i ) of the reaction between Bla Mab and nitrocefin was monitored for a range of substrate concentrations (1-500 μM) and relebactam concentrations (0, 0.5, 0.75, 1 and 2.5 μM). This data was plotted v i vs [S] in order to determine K m values (2c/f). The values for K obs were obtained as previously described ( Dubée et al., 2014 ) and plotted against relebactam concentrations ([I]) to deduce a carbamylation rate ( K 2 / K i ) for Bla Mab (2d). The kinetics of Bla Mab decarbamylation were assessed to show the recovery of nitrocefin hydrolysis by Bla Mab after inhibition by relebactam in order to derive a K off value (2e). Kinetic parameters were derived as described previously (2f) ( Dubée et al., 2014 ).
    Nitrocefin, supplied by carbosynth, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nitrocefin/product/carbosynth
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    nitrocefin - by Bioz Stars, 2020-09
    94/100 stars
      Buy from Supplier

    Image Search Results


    Kinetics of changes in E. coli ML-35p outer and cytoplasmic membrane permeability measured with the use of chromogenic markers – the products of nitrocefin (OD 540 ) and ONPG (OD 405 ) hydrolysis, respectively. Outer membrane permeability resulting from incubation of bacteria with mini-ChBac7.5Nα at various concentrations (from 0.125 to 32 μM, highlighted with colors) in the absence (A) or in the presence (B) of 0.9% NaCl. Melittin and ChMAP-28 at concentration of 1 μM were used as positive control samples. (C) Analysis of outer membrane permeability resulting from incubation with ChMAP-28 or melittin in the presence of 0.9% NaCl. (D) Comparative analysis of cytoplasmic membrane permeability resulting from incubation with the individual ChMAP-28 or with its combinations with mini-ChBac7.5Nα. Melittin at concentration of 8 μM was used as a positive control. Three independent experiments were performed, and the curve pattern was similar for the three series.

    Journal: Frontiers in Microbiology

    Article Title: Combined Antibacterial Effects of Goat Cathelicidins With Different Mechanisms of Action

    doi: 10.3389/fmicb.2018.02983

    Figure Lengend Snippet: Kinetics of changes in E. coli ML-35p outer and cytoplasmic membrane permeability measured with the use of chromogenic markers – the products of nitrocefin (OD 540 ) and ONPG (OD 405 ) hydrolysis, respectively. Outer membrane permeability resulting from incubation of bacteria with mini-ChBac7.5Nα at various concentrations (from 0.125 to 32 μM, highlighted with colors) in the absence (A) or in the presence (B) of 0.9% NaCl. Melittin and ChMAP-28 at concentration of 1 μM were used as positive control samples. (C) Analysis of outer membrane permeability resulting from incubation with ChMAP-28 or melittin in the presence of 0.9% NaCl. (D) Comparative analysis of cytoplasmic membrane permeability resulting from incubation with the individual ChMAP-28 or with its combinations with mini-ChBac7.5Nα. Melittin at concentration of 8 μM was used as a positive control. Three independent experiments were performed, and the curve pattern was similar for the three series.

    Article Snippet: Peptide samples were placed in the wells of a 96-well plate with non-binding surface (NBS, Corning #3641), and the optical density (OD) of the solution rising due to the appearance of the hydrolyzed nitrocefin or ONPG was measured at 540 and 405 nm, respectively, using the Multiskan EX microplate reader (Thermo Fisher Scientific).

    Techniques: Permeability, Incubation, Concentration Assay, Positive Control

    Nitrocefin degradation assay for E . coli ATCC 35218, 35218R and 35218Δ bla . Nitrocefin degradation by E . coli 35218R (red squares), E . coli ATCC 35218 (black circles) and E . coli 35218Δ bla (green triangles). The absorbance at 480 nm vs. time were modelled by linear regression and compared by curve fitting analysis (CFA). Strain 35218R (red squares and line) hydrolyzed nitrocefin more efficiently than the parental ATCC 35218 (black circles and line), while no hydrolysis occurred with the plasmid-cured isogenic strain 35218Δ bla (green diamonds and line), confirming β-lactamase hyperproduction by E . coli 35218R and negligible β-lactamase activity of E . coli 35218Δ bla . The P value from the slope comparison indicates that each group is described by an independent line.

    Journal: PLoS ONE

    Article Title: Impact on Bacterial Resistance of Therapeutically Nonequivalent Generics: The Case of Piperacillin-Tazobactam

    doi: 10.1371/journal.pone.0155806

    Figure Lengend Snippet: Nitrocefin degradation assay for E . coli ATCC 35218, 35218R and 35218Δ bla . Nitrocefin degradation by E . coli 35218R (red squares), E . coli ATCC 35218 (black circles) and E . coli 35218Δ bla (green triangles). The absorbance at 480 nm vs. time were modelled by linear regression and compared by curve fitting analysis (CFA). Strain 35218R (red squares and line) hydrolyzed nitrocefin more efficiently than the parental ATCC 35218 (black circles and line), while no hydrolysis occurred with the plasmid-cured isogenic strain 35218Δ bla (green diamonds and line), confirming β-lactamase hyperproduction by E . coli 35218R and negligible β-lactamase activity of E . coli 35218Δ bla . The P value from the slope comparison indicates that each group is described by an independent line.

    Article Snippet: The amount of nitrocefin degraded per minute was determined by Beer’s equation with the change in absorbance at OD486nm (Genesys 20 spectrophotometer, Thermo Scientific, USA) and a molar extinction coefficient of 20,500 M-1 .cm-1 , and the result was expressed as the nmol of nitrocefin degraded per minute per mg of protein [ ].

    Techniques: Degradation Assay, Plasmid Preparation, Activity Assay

    Changes in outer and inner membrane permeabilization caused by olanexidine and chlorhexidine in E. coli ML-35p cells incubated in 5 mM HEPES buffer containing 20 μg/ml nitrocefin and 5 μM carbonyl cyanide 3-chlorophenylhydrazone with olanexidine

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: Bactericidal Effects and Mechanism of Action of Olanexidine Gluconate, a New Antiseptic

    doi: 10.1128/AAC.05048-14

    Figure Lengend Snippet: Changes in outer and inner membrane permeabilization caused by olanexidine and chlorhexidine in E. coli ML-35p cells incubated in 5 mM HEPES buffer containing 20 μg/ml nitrocefin and 5 μM carbonyl cyanide 3-chlorophenylhydrazone with olanexidine

    Article Snippet: Bodipy TR cadaverine [5-(((4-(4,4-difluoro-5-(2-thienyl)-4-bora-3a,4a-diaza- s -indacene-3-yl)phenoxy)acetyl)amino)–pentylamine, hydrochloride] (BC), lipid A from Escherichia coli strain K-12, and nitrocefin were purchased from Thermo Fisher Scientific, Inc. (Waltham, MA), List Biological Laboratories, Inc. (Campbell, CA), and Merck KGaA (Darmstadt, Germany), respectively.

    Techniques: Incubation

    Biochemical analysis of relebactam inhibition of M. abscessus β -lactamase, Bla Mab . Our novel Thin Layer Chromatography (TLC) assay (2a) exhibiting the activity of Bla Mab in the turnover of penicillin V (high R f value) to penicilloic acid (lower R f value). In the absence, or termination of activity of Bla Mab (by boiling (100 °C) for 1 h) or addition of known inhibitor avibactam ( Lefebvre et al., 2017 ) (200 μg/mL) no lower R f value spot corresponding to penicilloic acid is seen on the TLC plate. The addition of relebactam to the reaction between Bla Mab and penicillin V also results in the absence of the lower Rf value spot, suggesting inhibition of Bla Mab (2a). This observed inhibition is validated by a spectrophotometric analysis, where the turnover of nitrocefin (100 μM) by Bla Mab (0.25 nM) was monitored at 486 nm with a varying concentration of relebactam (0, 0.1, 1, 10 and 100 μM). The increase in concentration of relebactam resulted in partial inhibition of nitrocefin turnover at 1 μM and total abrogation at 10 μM (2b). The initial velocity (v i ) of the reaction between Bla Mab and nitrocefin was monitored for a range of substrate concentrations (1-500 μM) and relebactam concentrations (0, 0.5, 0.75, 1 and 2.5 μM). This data was plotted v i vs [S] in order to determine K m values (2c/f). The values for K obs were obtained as previously described ( Dubée et al., 2014 ) and plotted against relebactam concentrations ([I]) to deduce a carbamylation rate ( K 2 / K i ) for Bla Mab (2d). The kinetics of Bla Mab decarbamylation were assessed to show the recovery of nitrocefin hydrolysis by Bla Mab after inhibition by relebactam in order to derive a K off value (2e). Kinetic parameters were derived as described previously (2f) ( Dubée et al., 2014 ).

    Journal: bioRxiv

    Article Title: Combining amoxicillin and relebactam provides a new therapeutic option for Mycobacterium abscessus infection

    doi: 10.1101/563189

    Figure Lengend Snippet: Biochemical analysis of relebactam inhibition of M. abscessus β -lactamase, Bla Mab . Our novel Thin Layer Chromatography (TLC) assay (2a) exhibiting the activity of Bla Mab in the turnover of penicillin V (high R f value) to penicilloic acid (lower R f value). In the absence, or termination of activity of Bla Mab (by boiling (100 °C) for 1 h) or addition of known inhibitor avibactam ( Lefebvre et al., 2017 ) (200 μg/mL) no lower R f value spot corresponding to penicilloic acid is seen on the TLC plate. The addition of relebactam to the reaction between Bla Mab and penicillin V also results in the absence of the lower Rf value spot, suggesting inhibition of Bla Mab (2a). This observed inhibition is validated by a spectrophotometric analysis, where the turnover of nitrocefin (100 μM) by Bla Mab (0.25 nM) was monitored at 486 nm with a varying concentration of relebactam (0, 0.1, 1, 10 and 100 μM). The increase in concentration of relebactam resulted in partial inhibition of nitrocefin turnover at 1 μM and total abrogation at 10 μM (2b). The initial velocity (v i ) of the reaction between Bla Mab and nitrocefin was monitored for a range of substrate concentrations (1-500 μM) and relebactam concentrations (0, 0.5, 0.75, 1 and 2.5 μM). This data was plotted v i vs [S] in order to determine K m values (2c/f). The values for K obs were obtained as previously described ( Dubée et al., 2014 ) and plotted against relebactam concentrations ([I]) to deduce a carbamylation rate ( K 2 / K i ) for Bla Mab (2d). The kinetics of Bla Mab decarbamylation were assessed to show the recovery of nitrocefin hydrolysis by Bla Mab after inhibition by relebactam in order to derive a K off value (2e). Kinetic parameters were derived as described previously (2f) ( Dubée et al., 2014 ).

    Article Snippet: The hydrolysis of nitrocefin was monitored at 486 nm using a Multiskan Go plate reader (Thermo Scientific).

    Techniques: Inhibition, Thin Layer Chromatography, Activity Assay, Concentration Assay, Derivative Assay