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Becton Dickinson nitrocefin disks
Detection of β -lactamase associated with outer membrane vesicles (OMVs) using <t>nitrocefin</t> disk. (1) Penicillin-sensitive Pm 12948 strain; (2) OMVs; and (3) penicillin-resistant Pm 12945 cells.
Nitrocefin Disks, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 88/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/nitrocefin disks/product/Becton Dickinson
Average 88 stars, based on 7 article reviews
Price from $9.99 to $1999.99
nitrocefin disks - by Bioz Stars, 2020-09
88/100 stars

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1) Product Images from "Outer membrane vesicles of Pasteurella multocida contain virulence factors"

Article Title: Outer membrane vesicles of Pasteurella multocida contain virulence factors

Journal: MicrobiologyOpen

doi: 10.1002/mbo3.201

Detection of β -lactamase associated with outer membrane vesicles (OMVs) using nitrocefin disk. (1) Penicillin-sensitive Pm 12948 strain; (2) OMVs; and (3) penicillin-resistant Pm 12945 cells.
Figure Legend Snippet: Detection of β -lactamase associated with outer membrane vesicles (OMVs) using nitrocefin disk. (1) Penicillin-sensitive Pm 12948 strain; (2) OMVs; and (3) penicillin-resistant Pm 12945 cells.

Techniques Used:

2) Product Images from "Outer membrane vesicles of Pasteurella multocida contain virulence factors"

Article Title: Outer membrane vesicles of Pasteurella multocida contain virulence factors

Journal: MicrobiologyOpen

doi: 10.1002/mbo3.201

Detection of β -lactamase associated with outer membrane vesicles (OMVs) using nitrocefin disk. (1) Penicillin-sensitive Pm 12948 strain; (2) OMVs; and (3) penicillin-resistant Pm 12945 cells.
Figure Legend Snippet: Detection of β -lactamase associated with outer membrane vesicles (OMVs) using nitrocefin disk. (1) Penicillin-sensitive Pm 12948 strain; (2) OMVs; and (3) penicillin-resistant Pm 12945 cells.

Techniques Used:

3) Product Images from "Outer membrane vesicles of Pasteurella multocida contain virulence factors"

Article Title: Outer membrane vesicles of Pasteurella multocida contain virulence factors

Journal: MicrobiologyOpen

doi: 10.1002/mbo3.201

Detection of β -lactamase associated with outer membrane vesicles (OMVs) using nitrocefin disk. (1) Penicillin-sensitive Pm 12948 strain; (2) OMVs; and (3) penicillin-resistant Pm 12945 cells.
Figure Legend Snippet: Detection of β -lactamase associated with outer membrane vesicles (OMVs) using nitrocefin disk. (1) Penicillin-sensitive Pm 12948 strain; (2) OMVs; and (3) penicillin-resistant Pm 12945 cells.

Techniques Used:

Related Articles

Incubation:

Article Title: Clinical and Microbiological Aspects of β-Lactam Resistance in Staphylococcus lugdunensis
Article Snippet: .. Isolates were tested for β-lactamase production using nitrocefin disks (BD) according to the manufacturer's recommendations, using cells that were subjected to cefoxitin induction after overnight incubation. .. Briefly, growth from the periphery of the cefoxitin disk diffusion zone was applied to a premoistened nitrocefin disk.

other:

Article Title: Antimicrobial susceptibility of Neisseria gonorrhoeae isolates in Vientiane, Lao PDR
Article Snippet: The tested antibiotics were ceftriaxone (30 μg), spectinomycin (100 μg), ciprofloxacin (5 μg), penicillin G (10 U) and tetracycline (30 μg). β-Lactamase detection was undertaken using Cefinase disks (BD BBL™; Zuellig Pharma, Bangkok, Thailand).

Article Title: Occurrence and characterization of methicillin-resistant staphylococci from bovine mastitis milk samples in Finland
Article Snippet: Beta-lactamase production by MRS isolates was tested with Cefinase disks (BD BBL Paper Disk for the Detection of β-Lactamase Enzymes, Becton-Dickinson, NJ, USA).

Article Title: Comparative Genomic Analysis of Vibrio diabolicus and Six Taxonomic Synonyms: A First Look at the Distribution and Diversity of the Expanded Species
Article Snippet: The production of β-lactamase was tested using Cefinase disks (Becton, Dickinson and Company, Franklin Lakes, NJ, United States) according to the manufacturer’s instructions.

Article Title: Outer membrane vesicles of Pasteurella multocida contain virulence factors
Article Snippet: Presence of active β -lactamase in OMVs from Pm Nitrocefin disks (Becton Dickinson of México, Cuautitlán Izcalli, México) were used to detect β -lactamase in Pm OMVs.

Activity Assay:

Article Title: Outer membrane vesicles of Pasteurella multocida contain virulence factors
Article Snippet: .. In order to know if β -lactamase was a contamination from cell debris, OMVs were treated with proteinase K, to digest β -lactamase associated with the outer membrane of the OMVs, and permeabilized with 0.02% Triton X-100 and β -lactamase activity was measured on nitrocefin disks as before. .. Although activity diminished highly, it does not disappear, suggesting that β -lactamase activity was present externally and inside of OMVs (data not shown).

Transmission Electron Microscopy:

Article Title: Nasopharyngeal Haemophilus influenzae Carriage in Japanese Children Attending Day-Care Centers ▿
Article Snippet: .. Three isolates were positive for β-lactamase, as detected with nitrocefin disks, and they all had the TEM-1-type β-lactamase gene. ..

Derivative Assay:

Article Title: Outer membrane vesicles of Pasteurella multocida contain virulence factors
Article Snippet: .. OMVs derived from Pm 12945 strain tested positive on nitrocefin disks but a strong response was observed when the bacterial cells were included. ..

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  • 93
    Becton Dickinson nitrocefin impregnated paper disks
    <t>Nitrocefin</t> PDI folding assay. TEM1 β-lactamase (Bla) and pPDI mutant TEM1 β-lactamase (MBla) were obtained by concentrating culture supernatants from strains RGP663 and RGP665, respectively. MBla contains consecutive disulfide bonds and
    Nitrocefin Impregnated Paper Disks, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nitrocefin impregnated paper disks/product/Becton Dickinson
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    nitrocefin impregnated paper disks - by Bioz Stars, 2020-09
    93/100 stars
      Buy from Supplier

    88
    Becton Dickinson nitrocefin disks
    Detection of β -lactamase associated with outer membrane vesicles (OMVs) using <t>nitrocefin</t> disk. (1) Penicillin-sensitive Pm 12948 strain; (2) OMVs; and (3) penicillin-resistant Pm 12945 cells.
    Nitrocefin Disks, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 88/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nitrocefin disks/product/Becton Dickinson
    Average 88 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    nitrocefin disks - by Bioz Stars, 2020-09
    88/100 stars
      Buy from Supplier

    Image Search Results


    Nitrocefin PDI folding assay. TEM1 β-lactamase (Bla) and pPDI mutant TEM1 β-lactamase (MBla) were obtained by concentrating culture supernatants from strains RGP663 and RGP665, respectively. MBla contains consecutive disulfide bonds and

    Journal: Journal of Bacteriology

    Article Title: Disulfide Bond Oxidoreductase DsbA2 of Legionella pneumophila Exhibits Protein Disulfide Isomerase Activity

    doi: 10.1128/JB.01949-12

    Figure Lengend Snippet: Nitrocefin PDI folding assay. TEM1 β-lactamase (Bla) and pPDI mutant TEM1 β-lactamase (MBla) were obtained by concentrating culture supernatants from strains RGP663 and RGP665, respectively. MBla contains consecutive disulfide bonds and

    Article Snippet: Bla activity was tested by spotting 5 μl of concentrated supernatant onto nitrocefin-impregnated paper disks (Becton, Dickinson).

    Techniques: Mutagenesis

    Detection of β -lactamase associated with outer membrane vesicles (OMVs) using nitrocefin disk. (1) Penicillin-sensitive Pm 12948 strain; (2) OMVs; and (3) penicillin-resistant Pm 12945 cells.

    Journal: MicrobiologyOpen

    Article Title: Outer membrane vesicles of Pasteurella multocida contain virulence factors

    doi: 10.1002/mbo3.201

    Figure Lengend Snippet: Detection of β -lactamase associated with outer membrane vesicles (OMVs) using nitrocefin disk. (1) Penicillin-sensitive Pm 12948 strain; (2) OMVs; and (3) penicillin-resistant Pm 12945 cells.

    Article Snippet: In order to know if β -lactamase was a contamination from cell debris, OMVs were treated with proteinase K, to digest β -lactamase associated with the outer membrane of the OMVs, and permeabilized with 0.02% Triton X-100 and β -lactamase activity was measured on nitrocefin disks as before.

    Techniques: