Journal: Scientific Reports
Article Title: Protease Inhibition Improves Healing of The Vaginal Wall after Obstetrical Injury: Results from a Preclinical Animal Model
Figure Lengend Snippet: Effect of injury on vaginal elastic fibers with injury ± actinonin. Microscopic images of vaginal muscularis on 3d postpartum in ( A ) injury + PBS ( Inj 3d ) and injury + actinonin ( Inj + Act 3d ). The 4–5 µm sections of full-thickness vaginal rings were stained with Hart’s stain. Quantitative analysis performed using a Nikon E1600 microscope with 40x objective and ImageJ software. Scale bars 50 µm. Graphical depiction represents ( B ) elastin fiber length (µm) and ( C ) percent area covered by elastic fibers in pregnant ( D21 ) controls compared to postpartum time points for injury with (triangles) and without actinonin (solid circles). n = 4–5 in all groups, *P
Article Snippet: Images of each Masson trichrome section were captured and analyzed using a Nikon E1600 microscope and Nikon NIS Elements AR software (Melville, NY).
Techniques: Staining, Microscopy, Software