nih3t3 l1  (ATCC)


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    Structured Review

    ATCC nih3t3 l1
    REV-ERBα binds to the promoters of Stra6, Crbp1, and Lrat . A, Western blot analysis for REV-ERBα protein expression in <t>NIH3T3-L1</t> pre-adipocytes with or without treatment with differentiation media for 2 h. B, ChIP assays determined by semi-quantitative PCR in NIH3T3-L1 cells treated with differentiation media for 2 h to analyze the Rev-erbα recruitment to ROREs at sites denoted as distance from start site on the genes indicated. C, quantification of ChIP performed in triplicate. Data are represented as mean ± S.E. * indicates significance compared with IgG control of at least p
    Nih3t3 L1, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nih3t3 l1/product/ATCC
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    nih3t3 l1 - by Bioz Stars, 2022-05
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    Images

    1) Product Images from "The retinol-binding protein receptor STRA6 regulates diurnal insulin responses"

    Article Title: The retinol-binding protein receptor STRA6 regulates diurnal insulin responses

    Journal: The Journal of Biological Chemistry

    doi: 10.1074/jbc.M117.782334

    REV-ERBα binds to the promoters of Stra6, Crbp1, and Lrat . A, Western blot analysis for REV-ERBα protein expression in NIH3T3-L1 pre-adipocytes with or without treatment with differentiation media for 2 h. B, ChIP assays determined by semi-quantitative PCR in NIH3T3-L1 cells treated with differentiation media for 2 h to analyze the Rev-erbα recruitment to ROREs at sites denoted as distance from start site on the genes indicated. C, quantification of ChIP performed in triplicate. Data are represented as mean ± S.E. * indicates significance compared with IgG control of at least p
    Figure Legend Snippet: REV-ERBα binds to the promoters of Stra6, Crbp1, and Lrat . A, Western blot analysis for REV-ERBα protein expression in NIH3T3-L1 pre-adipocytes with or without treatment with differentiation media for 2 h. B, ChIP assays determined by semi-quantitative PCR in NIH3T3-L1 cells treated with differentiation media for 2 h to analyze the Rev-erbα recruitment to ROREs at sites denoted as distance from start site on the genes indicated. C, quantification of ChIP performed in triplicate. Data are represented as mean ± S.E. * indicates significance compared with IgG control of at least p

    Techniques Used: Western Blot, Expressing, Chromatin Immunoprecipitation, Real-time Polymerase Chain Reaction

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    ATCC nih 3t3 l1 preadipocytes
    Influence of CAIII knockdown on the induction of adipogenesis in <t>NIH</t> <t>3T3-L1</t> preadipocytes
    Nih 3t3 L1 Preadipocytes, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nih 3t3 l1 preadipocytes/product/ATCC
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    nih 3t3 l1 preadipocytes - by Bioz Stars, 2022-05
    97/100 stars
      Buy from Supplier

    97
    ATCC nih3t3 l1
    REV-ERBα binds to the promoters of Stra6, Crbp1, and Lrat . A, Western blot analysis for REV-ERBα protein expression in <t>NIH3T3-L1</t> pre-adipocytes with or without treatment with differentiation media for 2 h. B, ChIP assays determined by semi-quantitative PCR in NIH3T3-L1 cells treated with differentiation media for 2 h to analyze the Rev-erbα recruitment to ROREs at sites denoted as distance from start site on the genes indicated. C, quantification of ChIP performed in triplicate. Data are represented as mean ± S.E. * indicates significance compared with IgG control of at least p
    Nih3t3 L1, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nih3t3 l1/product/ATCC
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    nih3t3 l1 - by Bioz Stars, 2022-05
    97/100 stars
      Buy from Supplier

    Image Search Results


    Influence of CAIII knockdown on the induction of adipogenesis in NIH 3T3-L1 preadipocytes

    Journal: Experimental Cell Research

    Article Title: Carbonic anhydrase III regulates peroxisome proliferator-activated receptor-?2

    doi: 10.1016/j.yexcr.2012.02.011

    Figure Lengend Snippet: Influence of CAIII knockdown on the induction of adipogenesis in NIH 3T3-L1 preadipocytes

    Article Snippet: We conclude that overexpression of CAIII in NIH 3T3-L1 preadipocytes has no substantive effect on adipogenic differentiation.

    Techniques:

    Analysis of Sp1, PEMT, and TBP protein levels by immunoblotting. 3T3-L1 fibroblasts were differentiated into adipocytes. Day 0 represents 2 days at post-confluence and the starting point of the differentiation protocol. Cells were harvested at the indicted

    Journal: The Journal of Biological Chemistry

    Article Title: A Role for Sp1 in Transcriptional Regulation of Phosphatidylethanolamine N-Methyltransferase in Liver and 3T3-L1 Adipocytes *

    doi: 10.1074/jbc.M110.109843

    Figure Lengend Snippet: Analysis of Sp1, PEMT, and TBP protein levels by immunoblotting. 3T3-L1 fibroblasts were differentiated into adipocytes. Day 0 represents 2 days at post-confluence and the starting point of the differentiation protocol. Cells were harvested at the indicted

    Article Snippet: To induce differentiation, NIH 3T3-L1 fibroblasts were cultivated in growth medium until confluent.

    Techniques:

    REV-ERBα binds to the promoters of Stra6, Crbp1, and Lrat . A, Western blot analysis for REV-ERBα protein expression in NIH3T3-L1 pre-adipocytes with or without treatment with differentiation media for 2 h. B, ChIP assays determined by semi-quantitative PCR in NIH3T3-L1 cells treated with differentiation media for 2 h to analyze the Rev-erbα recruitment to ROREs at sites denoted as distance from start site on the genes indicated. C, quantification of ChIP performed in triplicate. Data are represented as mean ± S.E. * indicates significance compared with IgG control of at least p

    Journal: The Journal of Biological Chemistry

    Article Title: The retinol-binding protein receptor STRA6 regulates diurnal insulin responses

    doi: 10.1074/jbc.M117.782334

    Figure Lengend Snippet: REV-ERBα binds to the promoters of Stra6, Crbp1, and Lrat . A, Western blot analysis for REV-ERBα protein expression in NIH3T3-L1 pre-adipocytes with or without treatment with differentiation media for 2 h. B, ChIP assays determined by semi-quantitative PCR in NIH3T3-L1 cells treated with differentiation media for 2 h to analyze the Rev-erbα recruitment to ROREs at sites denoted as distance from start site on the genes indicated. C, quantification of ChIP performed in triplicate. Data are represented as mean ± S.E. * indicates significance compared with IgG control of at least p

    Article Snippet: ChIP assays were performed in human 293T cells (ATCC) or NIH3T3-L1 (ATCC) mouse pre-adipocytes treated for 2 h with a differentiation media containing (DMEM + 10% fetal bovine serum, 10 μg/ml insulin, and 0.25 mmol/liter dexamethasone).

    Techniques: Western Blot, Expressing, Chromatin Immunoprecipitation, Real-time Polymerase Chain Reaction

    P311 gene silencing does not affect lipid accumulation in NIH3T3-L1 cells

    Journal:

    Article Title: P311 Functions in an Alternative Pathway of Lipid Accumulation Induced by Retinoic Acid

    doi: 10.1242/jcs.027151

    Figure Lengend Snippet: P311 gene silencing does not affect lipid accumulation in NIH3T3-L1 cells

    Article Snippet: For infection, NIH3T3-L1 cells (ATCC, Manassas, VA) were incubated with recombinant lentivirus in the presence of 4 µg/mL polybrene and subjected to centrifugation at room temperature for 90 minutes at 2500 rpm.

    Techniques: