nih3t3 l1  (ATCC)

Journal: Experimental Cell Research

Article Title: Carbonic anhydrase III regulates peroxisome proliferator-activated receptor-?2

doi: 10.1016/j.yexcr.2012.02.011

Figure Lengend Snippet: Influence of CAIII knockdown on the induction of adipogenesis in NIH 3T3-L1 preadipocytes

Article Snippet: We conclude that overexpression of CAIII in NIH 3T3-L1 preadipocytes has no substantive effect on adipogenic differentiation.

Techniques:

Journal: The Journal of Biological Chemistry

Article Title: A Role for Sp1 in Transcriptional Regulation of Phosphatidylethanolamine N-Methyltransferase in Liver and 3T3-L1 Adipocytes *

doi: 10.1074/jbc.M110.109843

Figure Lengend Snippet: Analysis of Sp1, PEMT, and TBP protein levels by immunoblotting. 3T3-L1 fibroblasts were differentiated into adipocytes. Day 0 represents 2 days at post-confluence and the starting point of the differentiation protocol. Cells were harvested at the indicted

Article Snippet: To induce differentiation, NIH 3T3-L1 fibroblasts were cultivated in growth medium until confluent.

Techniques:

Journal: The Journal of Biological Chemistry

Article Title: The retinol-binding protein receptor STRA6 regulates diurnal insulin responses

doi: 10.1074/jbc.M117.782334

Figure Lengend Snippet: REV-ERBα binds to the promoters of Stra6, Crbp1, and Lrat . A, Western blot analysis for REV-ERBα protein expression in NIH3T3-L1 pre-adipocytes with or without treatment with differentiation media for 2 h. B, ChIP assays determined by semi-quantitative PCR in NIH3T3-L1 cells treated with differentiation media for 2 h to analyze the Rev-erbα recruitment to ROREs at sites denoted as distance from start site on the genes indicated. C, quantification of ChIP performed in triplicate. Data are represented as mean ± S.E. * indicates significance compared with IgG control of at least p

Article Snippet: ChIP assays were performed in human 293T cells (ATCC) or NIH3T3-L1 (ATCC) mouse pre-adipocytes treated for 2 h with a differentiation media containing (DMEM + 10% fetal bovine serum, 10 μg/ml insulin, and 0.25 mmol/liter dexamethasone).

Techniques: Western Blot, Expressing, Chromatin Immunoprecipitation, Real-time Polymerase Chain Reaction

Journal:

Article Title: P311 Functions in an Alternative Pathway of Lipid Accumulation Induced by Retinoic Acid

doi: 10.1242/jcs.027151

Figure Lengend Snippet: P311 gene silencing does not affect lipid accumulation in NIH3T3-L1 cells

Article Snippet: For infection, NIH3T3-L1 cells (ATCC, Manassas, VA) were incubated with recombinant lentivirus in the presence of 4 µg/mL polybrene and subjected to centrifugation at room temperature for 90 minutes at 2500 rpm.

Techniques: