Journal: Aging and Disease

Article Title: The Flip Side of the Coin: METTL3 Serves as a Novel Cellular Senescence Accelerator via Negative Regulation of ITGA9

doi: 10.14336/AD.2024.1715

Figure Lengend Snippet: M6A modifications within total mRNA were significantly enhanced during the aging process . ( A ) Left: representative photographs of 2-month and 20-month-old male C57BL/6 mouse; Right: representative photographs of SA-β-gal staining in kidney tissues from young and old C57-BL/6 mice. ( B ) Left: representative photographs of SA-β-gal staining of P3 and P9 MEF cells; Right: the p16 expression levels in P3 and P9 MEF cells. ( C ) Left: representative photographs of SA-β-gal staining of H 2 O 2 -induced premature senescence of NIH/3T3 cells; Right: the p16 expression levels in H 2 O 2 treated NIH/3T3 cells. ( D ) Poly(A)+ RNA was extracted from the brain and liver tissue of young and old C57-BL/6 mice and subjected to RNA dot-blot analysis with an antibody recognizing m6A. Methylene blue staining served as the loading control. ( E ) Poly(A)+ RNA was extracted from the P3 and P9 MEF cells and subjected to RNA dot-blot analysis with an antibody recognizing m6A. Methylene blue staining served as the loading control. ( F ) Poly(A)+ RNA was extracted from the P3 and P9 MEF cells and subjected to RNA dot-blot analysis with an antibody recognizing m6A. Methylene blue staining served as the loading control. ( G ) Expression levels of m6A modification associated proteins were measured in P3 and P9 MEF cells, and the quantitative results were shown on the right. Mean (±SEM), n=4, passed normality test: Shapiro-Wilk test; One sample t test (MEF P9 vs 1, METTL3: p =0.0027, t = 9.176).

Article Snippet: NIH/3T3 fibroblasts were procured from the American Type Culture Collection (ATCC).

Techniques: Staining, Expressing, Dot Blot, Control, Modification

Journal: iScience

Article Title: Synthetic zipper mediated pre-targeting system for near-infrared photoimmunotherapy

doi: 10.1016/j.isci.2025.114558

Figure Lengend Snippet: The pre-targeting complex of NIR-PIT induces the release of ICD markers (A) Flow cytometric histograms show cell surface expression of calreticulin, HSP70, and HSP90 compared to untreated controls in SKOV3 cells with scFv-tisotumab-Zip2-Zip1-SNAP-IR700. (B) OVCAR3 cells treated with scFv-Sacituzumab-Zip2-Zip1-SNAP-IR700. (C) IGROV1 cells treated with scFv-Farletuzumab-Zip2-Zip1-SNAP-IR700. Only viable cells were included in the analysis. Bar graphs show the mean fluorescence intensity (MFI) of cell surface calreticulin, HSP70, and HSP90. Data are represented as mean ± SD from three biological replicates. Cells exposed to NIR light irradiation without incubation with NIR-PIT agents were used as control. Statistical significance was determined by a one-way ANOVA and Dunnett's test. ∗∗∗ p ≤ 0.001, ∗∗∗∗ p ≤ 0.0001.

Article Snippet: Ovarian cancer cell lines SKOV3 (HTB-77), OVCAR3 (HTB-161), IGROV1 (SCC-203), A2780 (ECACC-93112519), and HEK293T (CRL-11268) were obtained from the American Type Culture Collection, the European Collection of Authenticated Cell Cultures, and Sigma-Aldrich.

Techniques: Expressing, Fluorescence, Irradiation, Incubation, Control