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Yangtze River Pharmeceutical Group Co Ltd nifedipine
Selection and validation of the candidate biomarkers related to <t>nifedipine.</t> a) Comparison of gene expression profiles from nude mice tumors with the treatment of nifedipine or CMC-Na. Hierarchical clustering of nude mice tumors with different treatments. A dendrogram of the tumors was shown at the top. The changed genes were also clustered and gene names were on the right. Taken together, there were 51 up-regulated and 18 down-regulated genes found. Among them, there were 35 genes related to cell adhesion and migration. b) Transcript levels of differential genes in nude mice tumors treated with nifedipine or CMC-Na. Nine genes (BRI3, SMOC1, LLGL1, KCNC4, TRIM3, ATP2C1, DLG1, PDE4DIP and COL14A1) were found to have a consistent gain or loss in at least 10 tumor samples. The transcript levels were normalized to the expression of internal 18s rDNA. Data were presented as the mean ± SEM of three independent experiments. Specific comparison between nifedipine groups and control groups, P
Nifedipine, supplied by Yangtze River Pharmeceutical Group Co Ltd, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/nifedipine/product/Yangtze River Pharmeceutical Group Co Ltd
Average 92 stars, based on 1 article reviews
Price from $9.99 to $1999.99
nifedipine - by Bioz Stars, 2020-09
92/100 stars

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1) Product Images from "Nifedipine Promotes the Proliferation and Migration of Breast Cancer Cells"

Article Title: Nifedipine Promotes the Proliferation and Migration of Breast Cancer Cells

Journal: PLoS ONE

doi: 10.1371/journal.pone.0113649

Selection and validation of the candidate biomarkers related to nifedipine. a) Comparison of gene expression profiles from nude mice tumors with the treatment of nifedipine or CMC-Na. Hierarchical clustering of nude mice tumors with different treatments. A dendrogram of the tumors was shown at the top. The changed genes were also clustered and gene names were on the right. Taken together, there were 51 up-regulated and 18 down-regulated genes found. Among them, there were 35 genes related to cell adhesion and migration. b) Transcript levels of differential genes in nude mice tumors treated with nifedipine or CMC-Na. Nine genes (BRI3, SMOC1, LLGL1, KCNC4, TRIM3, ATP2C1, DLG1, PDE4DIP and COL14A1) were found to have a consistent gain or loss in at least 10 tumor samples. The transcript levels were normalized to the expression of internal 18s rDNA. Data were presented as the mean ± SEM of three independent experiments. Specific comparison between nifedipine groups and control groups, P
Figure Legend Snippet: Selection and validation of the candidate biomarkers related to nifedipine. a) Comparison of gene expression profiles from nude mice tumors with the treatment of nifedipine or CMC-Na. Hierarchical clustering of nude mice tumors with different treatments. A dendrogram of the tumors was shown at the top. The changed genes were also clustered and gene names were on the right. Taken together, there were 51 up-regulated and 18 down-regulated genes found. Among them, there were 35 genes related to cell adhesion and migration. b) Transcript levels of differential genes in nude mice tumors treated with nifedipine or CMC-Na. Nine genes (BRI3, SMOC1, LLGL1, KCNC4, TRIM3, ATP2C1, DLG1, PDE4DIP and COL14A1) were found to have a consistent gain or loss in at least 10 tumor samples. The transcript levels were normalized to the expression of internal 18s rDNA. Data were presented as the mean ± SEM of three independent experiments. Specific comparison between nifedipine groups and control groups, P

Techniques Used: Selection, Expressing, Mouse Assay, Migration

Inhibition of proliferation, migration and P-Erk of MDA-MB-231 by silencing BRI3 gene expression. a) Effective silencing of BRI3 mRNA in MDA-MB-231 cells after siRNA treatment. BRI3-homo-584 siRNA markedly inhibited BRI3 mRNA expression at 100 nM after transfection for 24h. b) siRNA directed against BRI3 suppressed the facilitation of nifedipine in the proliferation of MDA-MB-231 cells. Data were presented as the mean±SEM of three independent experiments. Specific comparison between nifedipine groups and control groups, P
Figure Legend Snippet: Inhibition of proliferation, migration and P-Erk of MDA-MB-231 by silencing BRI3 gene expression. a) Effective silencing of BRI3 mRNA in MDA-MB-231 cells after siRNA treatment. BRI3-homo-584 siRNA markedly inhibited BRI3 mRNA expression at 100 nM after transfection for 24h. b) siRNA directed against BRI3 suppressed the facilitation of nifedipine in the proliferation of MDA-MB-231 cells. Data were presented as the mean±SEM of three independent experiments. Specific comparison between nifedipine groups and control groups, P

Techniques Used: Inhibition, Migration, Multiple Displacement Amplification, Expressing, Transfection

miRNA-524-5p was target of nifedipine and regulated the expression of BRI3. a) Predicted duplex formation between human BRI3 3′-UTR (top) and three miRNA (bottom) including has-miRNA-524-5p, hsa-miR-1224-3p, hsa-miR-1229 by using TargetScan software. b) The expression of miRNA-524-5p decreased in the MDA-MB-231 cells treated with nifedipine for different periods. Results were presented as the mean±SEM of three independent experiments. P
Figure Legend Snippet: miRNA-524-5p was target of nifedipine and regulated the expression of BRI3. a) Predicted duplex formation between human BRI3 3′-UTR (top) and three miRNA (bottom) including has-miRNA-524-5p, hsa-miR-1224-3p, hsa-miR-1229 by using TargetScan software. b) The expression of miRNA-524-5p decreased in the MDA-MB-231 cells treated with nifedipine for different periods. Results were presented as the mean±SEM of three independent experiments. P

Techniques Used: Expressing, Software, Multiple Displacement Amplification

The effect of nifedipine on the nude mice in vivo. a) Nude mice were injected with 510 6 MDA-MB-231-GFP cells into the second fat pads to imitate the orthotopic breast cancer inoculation. After one week injection, when the breast tumor size reached 100mm 3 , nude mice were then fed with nifedipine (4.8 mg/kg) or CMC-Na. b) The tumor volume in nude mice described in (a) was measured at the indicated time points and plotted as the mean volume ± SEM; n = 10; P
Figure Legend Snippet: The effect of nifedipine on the nude mice in vivo. a) Nude mice were injected with 510 6 MDA-MB-231-GFP cells into the second fat pads to imitate the orthotopic breast cancer inoculation. After one week injection, when the breast tumor size reached 100mm 3 , nude mice were then fed with nifedipine (4.8 mg/kg) or CMC-Na. b) The tumor volume in nude mice described in (a) was measured at the indicated time points and plotted as the mean volume ± SEM; n = 10; P

Techniques Used: Mouse Assay, In Vivo, Injection, Multiple Displacement Amplification

Nifedipine stimulated the survival and migration of breast cancer cells invitro . a) Different concentrations of nifedipine could stimulate the proliferation of MDA-MB-231 cells. Results represented mean ± SEM of 3 independent experiments. P
Figure Legend Snippet: Nifedipine stimulated the survival and migration of breast cancer cells invitro . a) Different concentrations of nifedipine could stimulate the proliferation of MDA-MB-231 cells. Results represented mean ± SEM of 3 independent experiments. P

Techniques Used: Migration, Multiple Displacement Amplification

ERK activation in Nifedipine treated breast cancer cells. Phosphorylated Erk (P-Erk) immunoblotting in MDA-MB-231 cells treated with or without nifedipine at the indicated times. The level of phosphorylation was more intensive after treated with nifedipine for 40 minutes. Membranes were reprobed for GADPH for loading control. Gray value was analyzed by the Image J software. Results were represented the mean±SEM. n = 3; 1-way ANOVA. b) Phosphorylated Erk (P-Erk) immunoblotting in tumor tissues from nude mice treated with CMC-Na or nifedipine for 4 weeks. Membranes were reprobed for GADPH for loading control. The results were consistent with that in the MDA-MB-231 cells.
Figure Legend Snippet: ERK activation in Nifedipine treated breast cancer cells. Phosphorylated Erk (P-Erk) immunoblotting in MDA-MB-231 cells treated with or without nifedipine at the indicated times. The level of phosphorylation was more intensive after treated with nifedipine for 40 minutes. Membranes were reprobed for GADPH for loading control. Gray value was analyzed by the Image J software. Results were represented the mean±SEM. n = 3; 1-way ANOVA. b) Phosphorylated Erk (P-Erk) immunoblotting in tumor tissues from nude mice treated with CMC-Na or nifedipine for 4 weeks. Membranes were reprobed for GADPH for loading control. The results were consistent with that in the MDA-MB-231 cells.

Techniques Used: Activation Assay, Multiple Displacement Amplification, Software, Mouse Assay

Effect of nifedipine on intracellular calcium concentration in MDA-MB-231 cells. (a) MDA-MB-231 breast cancer cells were loaded with Furo-8 for 30 minutes, as described in “Materials and Methods”. Base-line [Ca 2+ ] i was recorded, followed by sequential additions of 1 uM nifedipine. No obvious changes were observed until the 150th picture was taken (n = 300). (b) No significant increase in [Ca 2+ ] i was observed by increasing the bath [K + ] from 2.5 mM to 90mM until the 150th picture was taken (n = 300).
Figure Legend Snippet: Effect of nifedipine on intracellular calcium concentration in MDA-MB-231 cells. (a) MDA-MB-231 breast cancer cells were loaded with Furo-8 for 30 minutes, as described in “Materials and Methods”. Base-line [Ca 2+ ] i was recorded, followed by sequential additions of 1 uM nifedipine. No obvious changes were observed until the 150th picture was taken (n = 300). (b) No significant increase in [Ca 2+ ] i was observed by increasing the bath [K + ] from 2.5 mM to 90mM until the 150th picture was taken (n = 300).

Techniques Used: Concentration Assay, Multiple Displacement Amplification

Related Articles

Injection:

Article Title: Nifedipine Promotes the Proliferation and Migration of Breast Cancer Cells
Article Snippet: .. One week after injection, when the breast tumor size reached 100mm3 , nude mice were then fed with nifedipine (4.8 mg/kg) (Yangtze River Pharmaceutical Group), verapamil (4.8 mg/kg) (Central Pharmaceutical Co., Ltd, China) by gauging. ..

Mouse Assay:

Article Title: Nifedipine Promotes the Proliferation and Migration of Breast Cancer Cells
Article Snippet: .. One week after injection, when the breast tumor size reached 100mm3 , nude mice were then fed with nifedipine (4.8 mg/kg) (Yangtze River Pharmaceutical Group), verapamil (4.8 mg/kg) (Central Pharmaceutical Co., Ltd, China) by gauging. ..

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    Yangtze River Pharmeceutical Group Co Ltd nifedipine
    Selection and validation of the candidate biomarkers related to <t>nifedipine.</t> a) Comparison of gene expression profiles from nude mice tumors with the treatment of nifedipine or CMC-Na. Hierarchical clustering of nude mice tumors with different treatments. A dendrogram of the tumors was shown at the top. The changed genes were also clustered and gene names were on the right. Taken together, there were 51 up-regulated and 18 down-regulated genes found. Among them, there were 35 genes related to cell adhesion and migration. b) Transcript levels of differential genes in nude mice tumors treated with nifedipine or CMC-Na. Nine genes (BRI3, SMOC1, LLGL1, KCNC4, TRIM3, ATP2C1, DLG1, PDE4DIP and COL14A1) were found to have a consistent gain or loss in at least 10 tumor samples. The transcript levels were normalized to the expression of internal 18s rDNA. Data were presented as the mean ± SEM of three independent experiments. Specific comparison between nifedipine groups and control groups, P
    Nifedipine, supplied by Yangtze River Pharmeceutical Group Co Ltd, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nifedipine/product/Yangtze River Pharmeceutical Group Co Ltd
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    nifedipine - by Bioz Stars, 2020-09
    92/100 stars
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    Yangtze River Pharmeceutical Group Co Ltd nifedipine sustained release tablets
    Selection and validation of the candidate biomarkers related to <t>nifedipine.</t> a) Comparison of gene expression profiles from nude mice tumors with the treatment of nifedipine or CMC-Na. Hierarchical clustering of nude mice tumors with different treatments. A dendrogram of the tumors was shown at the top. The changed genes were also clustered and gene names were on the right. Taken together, there were 51 up-regulated and 18 down-regulated genes found. Among them, there were 35 genes related to cell adhesion and migration. b) Transcript levels of differential genes in nude mice tumors treated with nifedipine or CMC-Na. Nine genes (BRI3, SMOC1, LLGL1, KCNC4, TRIM3, ATP2C1, DLG1, PDE4DIP and COL14A1) were found to have a consistent gain or loss in at least 10 tumor samples. The transcript levels were normalized to the expression of internal 18s rDNA. Data were presented as the mean ± SEM of three independent experiments. Specific comparison between nifedipine groups and control groups, P
    Nifedipine Sustained Release Tablets, supplied by Yangtze River Pharmeceutical Group Co Ltd, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nifedipine sustained release tablets/product/Yangtze River Pharmeceutical Group Co Ltd
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    nifedipine sustained release tablets - by Bioz Stars, 2020-09
    86/100 stars
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    Selection and validation of the candidate biomarkers related to nifedipine. a) Comparison of gene expression profiles from nude mice tumors with the treatment of nifedipine or CMC-Na. Hierarchical clustering of nude mice tumors with different treatments. A dendrogram of the tumors was shown at the top. The changed genes were also clustered and gene names were on the right. Taken together, there were 51 up-regulated and 18 down-regulated genes found. Among them, there were 35 genes related to cell adhesion and migration. b) Transcript levels of differential genes in nude mice tumors treated with nifedipine or CMC-Na. Nine genes (BRI3, SMOC1, LLGL1, KCNC4, TRIM3, ATP2C1, DLG1, PDE4DIP and COL14A1) were found to have a consistent gain or loss in at least 10 tumor samples. The transcript levels were normalized to the expression of internal 18s rDNA. Data were presented as the mean ± SEM of three independent experiments. Specific comparison between nifedipine groups and control groups, P

    Journal: PLoS ONE

    Article Title: Nifedipine Promotes the Proliferation and Migration of Breast Cancer Cells

    doi: 10.1371/journal.pone.0113649

    Figure Lengend Snippet: Selection and validation of the candidate biomarkers related to nifedipine. a) Comparison of gene expression profiles from nude mice tumors with the treatment of nifedipine or CMC-Na. Hierarchical clustering of nude mice tumors with different treatments. A dendrogram of the tumors was shown at the top. The changed genes were also clustered and gene names were on the right. Taken together, there were 51 up-regulated and 18 down-regulated genes found. Among them, there were 35 genes related to cell adhesion and migration. b) Transcript levels of differential genes in nude mice tumors treated with nifedipine or CMC-Na. Nine genes (BRI3, SMOC1, LLGL1, KCNC4, TRIM3, ATP2C1, DLG1, PDE4DIP and COL14A1) were found to have a consistent gain or loss in at least 10 tumor samples. The transcript levels were normalized to the expression of internal 18s rDNA. Data were presented as the mean ± SEM of three independent experiments. Specific comparison between nifedipine groups and control groups, P

    Article Snippet: One week after injection, when the breast tumor size reached 100mm3 , nude mice were then fed with nifedipine (4.8 mg/kg) (Yangtze River Pharmaceutical Group), verapamil (4.8 mg/kg) (Central Pharmaceutical Co., Ltd, China) by gauging.

    Techniques: Selection, Expressing, Mouse Assay, Migration

    Inhibition of proliferation, migration and P-Erk of MDA-MB-231 by silencing BRI3 gene expression. a) Effective silencing of BRI3 mRNA in MDA-MB-231 cells after siRNA treatment. BRI3-homo-584 siRNA markedly inhibited BRI3 mRNA expression at 100 nM after transfection for 24h. b) siRNA directed against BRI3 suppressed the facilitation of nifedipine in the proliferation of MDA-MB-231 cells. Data were presented as the mean±SEM of three independent experiments. Specific comparison between nifedipine groups and control groups, P

    Journal: PLoS ONE

    Article Title: Nifedipine Promotes the Proliferation and Migration of Breast Cancer Cells

    doi: 10.1371/journal.pone.0113649

    Figure Lengend Snippet: Inhibition of proliferation, migration and P-Erk of MDA-MB-231 by silencing BRI3 gene expression. a) Effective silencing of BRI3 mRNA in MDA-MB-231 cells after siRNA treatment. BRI3-homo-584 siRNA markedly inhibited BRI3 mRNA expression at 100 nM after transfection for 24h. b) siRNA directed against BRI3 suppressed the facilitation of nifedipine in the proliferation of MDA-MB-231 cells. Data were presented as the mean±SEM of three independent experiments. Specific comparison between nifedipine groups and control groups, P

    Article Snippet: One week after injection, when the breast tumor size reached 100mm3 , nude mice were then fed with nifedipine (4.8 mg/kg) (Yangtze River Pharmaceutical Group), verapamil (4.8 mg/kg) (Central Pharmaceutical Co., Ltd, China) by gauging.

    Techniques: Inhibition, Migration, Multiple Displacement Amplification, Expressing, Transfection

    miRNA-524-5p was target of nifedipine and regulated the expression of BRI3. a) Predicted duplex formation between human BRI3 3′-UTR (top) and three miRNA (bottom) including has-miRNA-524-5p, hsa-miR-1224-3p, hsa-miR-1229 by using TargetScan software. b) The expression of miRNA-524-5p decreased in the MDA-MB-231 cells treated with nifedipine for different periods. Results were presented as the mean±SEM of three independent experiments. P

    Journal: PLoS ONE

    Article Title: Nifedipine Promotes the Proliferation and Migration of Breast Cancer Cells

    doi: 10.1371/journal.pone.0113649

    Figure Lengend Snippet: miRNA-524-5p was target of nifedipine and regulated the expression of BRI3. a) Predicted duplex formation between human BRI3 3′-UTR (top) and three miRNA (bottom) including has-miRNA-524-5p, hsa-miR-1224-3p, hsa-miR-1229 by using TargetScan software. b) The expression of miRNA-524-5p decreased in the MDA-MB-231 cells treated with nifedipine for different periods. Results were presented as the mean±SEM of three independent experiments. P

    Article Snippet: One week after injection, when the breast tumor size reached 100mm3 , nude mice were then fed with nifedipine (4.8 mg/kg) (Yangtze River Pharmaceutical Group), verapamil (4.8 mg/kg) (Central Pharmaceutical Co., Ltd, China) by gauging.

    Techniques: Expressing, Software, Multiple Displacement Amplification

    The effect of nifedipine on the nude mice in vivo. a) Nude mice were injected with 510 6 MDA-MB-231-GFP cells into the second fat pads to imitate the orthotopic breast cancer inoculation. After one week injection, when the breast tumor size reached 100mm 3 , nude mice were then fed with nifedipine (4.8 mg/kg) or CMC-Na. b) The tumor volume in nude mice described in (a) was measured at the indicated time points and plotted as the mean volume ± SEM; n = 10; P

    Journal: PLoS ONE

    Article Title: Nifedipine Promotes the Proliferation and Migration of Breast Cancer Cells

    doi: 10.1371/journal.pone.0113649

    Figure Lengend Snippet: The effect of nifedipine on the nude mice in vivo. a) Nude mice were injected with 510 6 MDA-MB-231-GFP cells into the second fat pads to imitate the orthotopic breast cancer inoculation. After one week injection, when the breast tumor size reached 100mm 3 , nude mice were then fed with nifedipine (4.8 mg/kg) or CMC-Na. b) The tumor volume in nude mice described in (a) was measured at the indicated time points and plotted as the mean volume ± SEM; n = 10; P

    Article Snippet: One week after injection, when the breast tumor size reached 100mm3 , nude mice were then fed with nifedipine (4.8 mg/kg) (Yangtze River Pharmaceutical Group), verapamil (4.8 mg/kg) (Central Pharmaceutical Co., Ltd, China) by gauging.

    Techniques: Mouse Assay, In Vivo, Injection, Multiple Displacement Amplification

    Nifedipine stimulated the survival and migration of breast cancer cells invitro . a) Different concentrations of nifedipine could stimulate the proliferation of MDA-MB-231 cells. Results represented mean ± SEM of 3 independent experiments. P

    Journal: PLoS ONE

    Article Title: Nifedipine Promotes the Proliferation and Migration of Breast Cancer Cells

    doi: 10.1371/journal.pone.0113649

    Figure Lengend Snippet: Nifedipine stimulated the survival and migration of breast cancer cells invitro . a) Different concentrations of nifedipine could stimulate the proliferation of MDA-MB-231 cells. Results represented mean ± SEM of 3 independent experiments. P

    Article Snippet: One week after injection, when the breast tumor size reached 100mm3 , nude mice were then fed with nifedipine (4.8 mg/kg) (Yangtze River Pharmaceutical Group), verapamil (4.8 mg/kg) (Central Pharmaceutical Co., Ltd, China) by gauging.

    Techniques: Migration, Multiple Displacement Amplification

    ERK activation in Nifedipine treated breast cancer cells. Phosphorylated Erk (P-Erk) immunoblotting in MDA-MB-231 cells treated with or without nifedipine at the indicated times. The level of phosphorylation was more intensive after treated with nifedipine for 40 minutes. Membranes were reprobed for GADPH for loading control. Gray value was analyzed by the Image J software. Results were represented the mean±SEM. n = 3; 1-way ANOVA. b) Phosphorylated Erk (P-Erk) immunoblotting in tumor tissues from nude mice treated with CMC-Na or nifedipine for 4 weeks. Membranes were reprobed for GADPH for loading control. The results were consistent with that in the MDA-MB-231 cells.

    Journal: PLoS ONE

    Article Title: Nifedipine Promotes the Proliferation and Migration of Breast Cancer Cells

    doi: 10.1371/journal.pone.0113649

    Figure Lengend Snippet: ERK activation in Nifedipine treated breast cancer cells. Phosphorylated Erk (P-Erk) immunoblotting in MDA-MB-231 cells treated with or without nifedipine at the indicated times. The level of phosphorylation was more intensive after treated with nifedipine for 40 minutes. Membranes were reprobed for GADPH for loading control. Gray value was analyzed by the Image J software. Results were represented the mean±SEM. n = 3; 1-way ANOVA. b) Phosphorylated Erk (P-Erk) immunoblotting in tumor tissues from nude mice treated with CMC-Na or nifedipine for 4 weeks. Membranes were reprobed for GADPH for loading control. The results were consistent with that in the MDA-MB-231 cells.

    Article Snippet: One week after injection, when the breast tumor size reached 100mm3 , nude mice were then fed with nifedipine (4.8 mg/kg) (Yangtze River Pharmaceutical Group), verapamil (4.8 mg/kg) (Central Pharmaceutical Co., Ltd, China) by gauging.

    Techniques: Activation Assay, Multiple Displacement Amplification, Software, Mouse Assay

    Effect of nifedipine on intracellular calcium concentration in MDA-MB-231 cells. (a) MDA-MB-231 breast cancer cells were loaded with Furo-8 for 30 minutes, as described in “Materials and Methods”. Base-line [Ca 2+ ] i was recorded, followed by sequential additions of 1 uM nifedipine. No obvious changes were observed until the 150th picture was taken (n = 300). (b) No significant increase in [Ca 2+ ] i was observed by increasing the bath [K + ] from 2.5 mM to 90mM until the 150th picture was taken (n = 300).

    Journal: PLoS ONE

    Article Title: Nifedipine Promotes the Proliferation and Migration of Breast Cancer Cells

    doi: 10.1371/journal.pone.0113649

    Figure Lengend Snippet: Effect of nifedipine on intracellular calcium concentration in MDA-MB-231 cells. (a) MDA-MB-231 breast cancer cells were loaded with Furo-8 for 30 minutes, as described in “Materials and Methods”. Base-line [Ca 2+ ] i was recorded, followed by sequential additions of 1 uM nifedipine. No obvious changes were observed until the 150th picture was taken (n = 300). (b) No significant increase in [Ca 2+ ] i was observed by increasing the bath [K + ] from 2.5 mM to 90mM until the 150th picture was taken (n = 300).

    Article Snippet: One week after injection, when the breast tumor size reached 100mm3 , nude mice were then fed with nifedipine (4.8 mg/kg) (Yangtze River Pharmaceutical Group), verapamil (4.8 mg/kg) (Central Pharmaceutical Co., Ltd, China) by gauging.

    Techniques: Concentration Assay, Multiple Displacement Amplification

    Selection and validation of the candidate biomarkers related to nifedipine. a) Comparison of gene expression profiles from nude mice tumors with the treatment of nifedipine or CMC-Na. Hierarchical clustering of nude mice tumors with different treatments. A dendrogram of the tumors was shown at the top. The changed genes were also clustered and gene names were on the right. Taken together, there were 51 up-regulated and 18 down-regulated genes found. Among them, there were 35 genes related to cell adhesion and migration. b) Transcript levels of differential genes in nude mice tumors treated with nifedipine or CMC-Na. Nine genes (BRI3, SMOC1, LLGL1, KCNC4, TRIM3, ATP2C1, DLG1, PDE4DIP and COL14A1) were found to have a consistent gain or loss in at least 10 tumor samples. The transcript levels were normalized to the expression of internal 18s rDNA. Data were presented as the mean ± SEM of three independent experiments. Specific comparison between nifedipine groups and control groups, P

    Journal: PLoS ONE

    Article Title: Nifedipine Promotes the Proliferation and Migration of Breast Cancer Cells

    doi: 10.1371/journal.pone.0113649

    Figure Lengend Snippet: Selection and validation of the candidate biomarkers related to nifedipine. a) Comparison of gene expression profiles from nude mice tumors with the treatment of nifedipine or CMC-Na. Hierarchical clustering of nude mice tumors with different treatments. A dendrogram of the tumors was shown at the top. The changed genes were also clustered and gene names were on the right. Taken together, there were 51 up-regulated and 18 down-regulated genes found. Among them, there were 35 genes related to cell adhesion and migration. b) Transcript levels of differential genes in nude mice tumors treated with nifedipine or CMC-Na. Nine genes (BRI3, SMOC1, LLGL1, KCNC4, TRIM3, ATP2C1, DLG1, PDE4DIP and COL14A1) were found to have a consistent gain or loss in at least 10 tumor samples. The transcript levels were normalized to the expression of internal 18s rDNA. Data were presented as the mean ± SEM of three independent experiments. Specific comparison between nifedipine groups and control groups, P

    Article Snippet: Nifedipine (Sigma), verapamil (Sigma), MTT (5 mg/ml, Sigma), Paraformaldehyde (4%, Solarbio), Crystal violet (0.1%, Solarbio), RIPA (Solarbio), PMSF (Solarbio), Cocktail (25x, Roche), Fura-8™ (AAT Bioquest), DMSO (Sigma), CMC-Na (Beijing Chemical Technology, China), Nifedipine Sustained-Release Tablets (Yangtze River Pharmaceutical Group, China), Verapamil Hydrochloride Tablets (Central Pharmaceutical Co., Ltd, China).

    Techniques: Selection, Expressing, Mouse Assay, Migration

    Inhibition of proliferation, migration and P-Erk of MDA-MB-231 by silencing BRI3 gene expression. a) Effective silencing of BRI3 mRNA in MDA-MB-231 cells after siRNA treatment. BRI3-homo-584 siRNA markedly inhibited BRI3 mRNA expression at 100 nM after transfection for 24h. b) siRNA directed against BRI3 suppressed the facilitation of nifedipine in the proliferation of MDA-MB-231 cells. Data were presented as the mean±SEM of three independent experiments. Specific comparison between nifedipine groups and control groups, P

    Journal: PLoS ONE

    Article Title: Nifedipine Promotes the Proliferation and Migration of Breast Cancer Cells

    doi: 10.1371/journal.pone.0113649

    Figure Lengend Snippet: Inhibition of proliferation, migration and P-Erk of MDA-MB-231 by silencing BRI3 gene expression. a) Effective silencing of BRI3 mRNA in MDA-MB-231 cells after siRNA treatment. BRI3-homo-584 siRNA markedly inhibited BRI3 mRNA expression at 100 nM after transfection for 24h. b) siRNA directed against BRI3 suppressed the facilitation of nifedipine in the proliferation of MDA-MB-231 cells. Data were presented as the mean±SEM of three independent experiments. Specific comparison between nifedipine groups and control groups, P

    Article Snippet: Nifedipine (Sigma), verapamil (Sigma), MTT (5 mg/ml, Sigma), Paraformaldehyde (4%, Solarbio), Crystal violet (0.1%, Solarbio), RIPA (Solarbio), PMSF (Solarbio), Cocktail (25x, Roche), Fura-8™ (AAT Bioquest), DMSO (Sigma), CMC-Na (Beijing Chemical Technology, China), Nifedipine Sustained-Release Tablets (Yangtze River Pharmaceutical Group, China), Verapamil Hydrochloride Tablets (Central Pharmaceutical Co., Ltd, China).

    Techniques: Inhibition, Migration, Multiple Displacement Amplification, Expressing, Transfection

    miRNA-524-5p was target of nifedipine and regulated the expression of BRI3. a) Predicted duplex formation between human BRI3 3′-UTR (top) and three miRNA (bottom) including has-miRNA-524-5p, hsa-miR-1224-3p, hsa-miR-1229 by using TargetScan software. b) The expression of miRNA-524-5p decreased in the MDA-MB-231 cells treated with nifedipine for different periods. Results were presented as the mean±SEM of three independent experiments. P

    Journal: PLoS ONE

    Article Title: Nifedipine Promotes the Proliferation and Migration of Breast Cancer Cells

    doi: 10.1371/journal.pone.0113649

    Figure Lengend Snippet: miRNA-524-5p was target of nifedipine and regulated the expression of BRI3. a) Predicted duplex formation between human BRI3 3′-UTR (top) and three miRNA (bottom) including has-miRNA-524-5p, hsa-miR-1224-3p, hsa-miR-1229 by using TargetScan software. b) The expression of miRNA-524-5p decreased in the MDA-MB-231 cells treated with nifedipine for different periods. Results were presented as the mean±SEM of three independent experiments. P

    Article Snippet: Nifedipine (Sigma), verapamil (Sigma), MTT (5 mg/ml, Sigma), Paraformaldehyde (4%, Solarbio), Crystal violet (0.1%, Solarbio), RIPA (Solarbio), PMSF (Solarbio), Cocktail (25x, Roche), Fura-8™ (AAT Bioquest), DMSO (Sigma), CMC-Na (Beijing Chemical Technology, China), Nifedipine Sustained-Release Tablets (Yangtze River Pharmaceutical Group, China), Verapamil Hydrochloride Tablets (Central Pharmaceutical Co., Ltd, China).

    Techniques: Expressing, Software, Multiple Displacement Amplification

    The effect of nifedipine on the nude mice in vivo. a) Nude mice were injected with 510 6 MDA-MB-231-GFP cells into the second fat pads to imitate the orthotopic breast cancer inoculation. After one week injection, when the breast tumor size reached 100mm 3 , nude mice were then fed with nifedipine (4.8 mg/kg) or CMC-Na. b) The tumor volume in nude mice described in (a) was measured at the indicated time points and plotted as the mean volume ± SEM; n = 10; P

    Journal: PLoS ONE

    Article Title: Nifedipine Promotes the Proliferation and Migration of Breast Cancer Cells

    doi: 10.1371/journal.pone.0113649

    Figure Lengend Snippet: The effect of nifedipine on the nude mice in vivo. a) Nude mice were injected with 510 6 MDA-MB-231-GFP cells into the second fat pads to imitate the orthotopic breast cancer inoculation. After one week injection, when the breast tumor size reached 100mm 3 , nude mice were then fed with nifedipine (4.8 mg/kg) or CMC-Na. b) The tumor volume in nude mice described in (a) was measured at the indicated time points and plotted as the mean volume ± SEM; n = 10; P

    Article Snippet: Nifedipine (Sigma), verapamil (Sigma), MTT (5 mg/ml, Sigma), Paraformaldehyde (4%, Solarbio), Crystal violet (0.1%, Solarbio), RIPA (Solarbio), PMSF (Solarbio), Cocktail (25x, Roche), Fura-8™ (AAT Bioquest), DMSO (Sigma), CMC-Na (Beijing Chemical Technology, China), Nifedipine Sustained-Release Tablets (Yangtze River Pharmaceutical Group, China), Verapamil Hydrochloride Tablets (Central Pharmaceutical Co., Ltd, China).

    Techniques: Mouse Assay, In Vivo, Injection, Multiple Displacement Amplification

    Nifedipine stimulated the survival and migration of breast cancer cells invitro . a) Different concentrations of nifedipine could stimulate the proliferation of MDA-MB-231 cells. Results represented mean ± SEM of 3 independent experiments. P

    Journal: PLoS ONE

    Article Title: Nifedipine Promotes the Proliferation and Migration of Breast Cancer Cells

    doi: 10.1371/journal.pone.0113649

    Figure Lengend Snippet: Nifedipine stimulated the survival and migration of breast cancer cells invitro . a) Different concentrations of nifedipine could stimulate the proliferation of MDA-MB-231 cells. Results represented mean ± SEM of 3 independent experiments. P

    Article Snippet: Nifedipine (Sigma), verapamil (Sigma), MTT (5 mg/ml, Sigma), Paraformaldehyde (4%, Solarbio), Crystal violet (0.1%, Solarbio), RIPA (Solarbio), PMSF (Solarbio), Cocktail (25x, Roche), Fura-8™ (AAT Bioquest), DMSO (Sigma), CMC-Na (Beijing Chemical Technology, China), Nifedipine Sustained-Release Tablets (Yangtze River Pharmaceutical Group, China), Verapamil Hydrochloride Tablets (Central Pharmaceutical Co., Ltd, China).

    Techniques: Migration, Multiple Displacement Amplification

    ERK activation in Nifedipine treated breast cancer cells. Phosphorylated Erk (P-Erk) immunoblotting in MDA-MB-231 cells treated with or without nifedipine at the indicated times. The level of phosphorylation was more intensive after treated with nifedipine for 40 minutes. Membranes were reprobed for GADPH for loading control. Gray value was analyzed by the Image J software. Results were represented the mean±SEM. n = 3; 1-way ANOVA. b) Phosphorylated Erk (P-Erk) immunoblotting in tumor tissues from nude mice treated with CMC-Na or nifedipine for 4 weeks. Membranes were reprobed for GADPH for loading control. The results were consistent with that in the MDA-MB-231 cells.

    Journal: PLoS ONE

    Article Title: Nifedipine Promotes the Proliferation and Migration of Breast Cancer Cells

    doi: 10.1371/journal.pone.0113649

    Figure Lengend Snippet: ERK activation in Nifedipine treated breast cancer cells. Phosphorylated Erk (P-Erk) immunoblotting in MDA-MB-231 cells treated with or without nifedipine at the indicated times. The level of phosphorylation was more intensive after treated with nifedipine for 40 minutes. Membranes were reprobed for GADPH for loading control. Gray value was analyzed by the Image J software. Results were represented the mean±SEM. n = 3; 1-way ANOVA. b) Phosphorylated Erk (P-Erk) immunoblotting in tumor tissues from nude mice treated with CMC-Na or nifedipine for 4 weeks. Membranes were reprobed for GADPH for loading control. The results were consistent with that in the MDA-MB-231 cells.

    Article Snippet: Nifedipine (Sigma), verapamil (Sigma), MTT (5 mg/ml, Sigma), Paraformaldehyde (4%, Solarbio), Crystal violet (0.1%, Solarbio), RIPA (Solarbio), PMSF (Solarbio), Cocktail (25x, Roche), Fura-8™ (AAT Bioquest), DMSO (Sigma), CMC-Na (Beijing Chemical Technology, China), Nifedipine Sustained-Release Tablets (Yangtze River Pharmaceutical Group, China), Verapamil Hydrochloride Tablets (Central Pharmaceutical Co., Ltd, China).

    Techniques: Activation Assay, Multiple Displacement Amplification, Software, Mouse Assay

    Effect of nifedipine on intracellular calcium concentration in MDA-MB-231 cells. (a) MDA-MB-231 breast cancer cells were loaded with Furo-8 for 30 minutes, as described in “Materials and Methods”. Base-line [Ca 2+ ] i was recorded, followed by sequential additions of 1 uM nifedipine. No obvious changes were observed until the 150th picture was taken (n = 300). (b) No significant increase in [Ca 2+ ] i was observed by increasing the bath [K + ] from 2.5 mM to 90mM until the 150th picture was taken (n = 300).

    Journal: PLoS ONE

    Article Title: Nifedipine Promotes the Proliferation and Migration of Breast Cancer Cells

    doi: 10.1371/journal.pone.0113649

    Figure Lengend Snippet: Effect of nifedipine on intracellular calcium concentration in MDA-MB-231 cells. (a) MDA-MB-231 breast cancer cells were loaded with Furo-8 for 30 minutes, as described in “Materials and Methods”. Base-line [Ca 2+ ] i was recorded, followed by sequential additions of 1 uM nifedipine. No obvious changes were observed until the 150th picture was taken (n = 300). (b) No significant increase in [Ca 2+ ] i was observed by increasing the bath [K + ] from 2.5 mM to 90mM until the 150th picture was taken (n = 300).

    Article Snippet: Nifedipine (Sigma), verapamil (Sigma), MTT (5 mg/ml, Sigma), Paraformaldehyde (4%, Solarbio), Crystal violet (0.1%, Solarbio), RIPA (Solarbio), PMSF (Solarbio), Cocktail (25x, Roche), Fura-8™ (AAT Bioquest), DMSO (Sigma), CMC-Na (Beijing Chemical Technology, China), Nifedipine Sustained-Release Tablets (Yangtze River Pharmaceutical Group, China), Verapamil Hydrochloride Tablets (Central Pharmaceutical Co., Ltd, China).

    Techniques: Concentration Assay, Multiple Displacement Amplification