Structured Review

Biomol GmbH nifedipine
High voltage activation calcium channels' L-type regulation and pathway signaling Ca 2+ /CaM-K II affect GH release in bovine somatotropes. (a) Bar graph illustrating the regulation of hormone release by GHRP-6 (100 nM) applied alone or in the presence of <t>nifedipine</t> (0.5 μ M) a blocker of HVACC L-type. (b) Average amount of GH released in the absences (control) and after treatment with the signaling pathway Ca 2+ /CaM-K II inhibitor KN-62 (10 μ M) alone or in combination with GHRP-6 (100 nM). Each value represents the mean ± SE of determinations performed in triplicate from three independent experiments. The asterisks denote significant differences ( P
Nifedipine, supplied by Biomol GmbH, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/nifedipine/product/Biomol GmbH
Average 92 stars, based on 3 article reviews
Price from $9.99 to $1999.99
nifedipine - by Bioz Stars, 2020-09
92/100 stars

Images

1) Product Images from "Upregulation of Voltage-Gated Calcium Channel Cav1.3 in Bovine Somatotropes Treated with Ghrelin"

Article Title: Upregulation of Voltage-Gated Calcium Channel Cav1.3 in Bovine Somatotropes Treated with Ghrelin

Journal: Journal of Signal Transduction

doi: 10.1155/2013/527253

High voltage activation calcium channels' L-type regulation and pathway signaling Ca 2+ /CaM-K II affect GH release in bovine somatotropes. (a) Bar graph illustrating the regulation of hormone release by GHRP-6 (100 nM) applied alone or in the presence of nifedipine (0.5 μ M) a blocker of HVACC L-type. (b) Average amount of GH released in the absences (control) and after treatment with the signaling pathway Ca 2+ /CaM-K II inhibitor KN-62 (10 μ M) alone or in combination with GHRP-6 (100 nM). Each value represents the mean ± SE of determinations performed in triplicate from three independent experiments. The asterisks denote significant differences ( P
Figure Legend Snippet: High voltage activation calcium channels' L-type regulation and pathway signaling Ca 2+ /CaM-K II affect GH release in bovine somatotropes. (a) Bar graph illustrating the regulation of hormone release by GHRP-6 (100 nM) applied alone or in the presence of nifedipine (0.5 μ M) a blocker of HVACC L-type. (b) Average amount of GH released in the absences (control) and after treatment with the signaling pathway Ca 2+ /CaM-K II inhibitor KN-62 (10 μ M) alone or in combination with GHRP-6 (100 nM). Each value represents the mean ± SE of determinations performed in triplicate from three independent experiments. The asterisks denote significant differences ( P

Techniques Used: Activation Assay, Chick Chorioallantoic Membrane Assay

2) Product Images from "Blocking L-type Calcium Channels Reduced the Threshold of Cyclic AMP-induced Steroidogenic Acute Regulatory Gene Expression in MA-10 Mouse Leydig Cells"

Article Title: Blocking L-type Calcium Channels Reduced the Threshold of Cyclic AMP-induced Steroidogenic Acute Regulatory Gene Expression in MA-10 Mouse Leydig Cells

Journal: The Journal of endocrinology

doi: 10.1677/JOE-09-0206

Blocking Ca 2+ influx through L-type Ca 2+ channel enhanced cAMP-induced steroidogenesis and StAR protein expression in MA-10 mouse Leydig cells. MA-10 cells were cultured in serum-free Waymouth’s medium with increasing concentrations of the selective L-type Ca 2+ channel blocker, nifedipine, for 30 min and then 0.05 mM dbcAMP was added to the culture for 6 h. A , the cells were collected and 20μg of cell lysate protein was used for Western blot analysis of StAR protein. B , progesterone concentration in the medium was assessed by RIA. **, p
Figure Legend Snippet: Blocking Ca 2+ influx through L-type Ca 2+ channel enhanced cAMP-induced steroidogenesis and StAR protein expression in MA-10 mouse Leydig cells. MA-10 cells were cultured in serum-free Waymouth’s medium with increasing concentrations of the selective L-type Ca 2+ channel blocker, nifedipine, for 30 min and then 0.05 mM dbcAMP was added to the culture for 6 h. A , the cells were collected and 20μg of cell lysate protein was used for Western blot analysis of StAR protein. B , progesterone concentration in the medium was assessed by RIA. **, p

Techniques Used: Blocking Assay, Expressing, Cell Culture, Western Blot, Concentration Assay

Blocking Ca 2+ influx through L-type Ca 2+ channel enhanced cAMP-induced Star gene transcription in MA-10 mouse Leydig cells. MA-10 cells were cultured in serum-free Waymouth’s medium with increasing concentrations of the selective L-type Ca 2+ channel blocker, nifedipine, for 30 min and then 0.05 mM dbcAMP was added to the culture for 6 h. A, the cells were collected for total RNA isolation and Star mRNA was analyzed by RT-PCR with β-actin as an internal marker. B, MA-10 cells were transfected with a Star promoter/luciferase plasmid (PGL2/ Star ) and a pRLSV40 vector, a plasmid that constitutively expresses Renilla luciferase. The cells were then treated as described above and the cell lysate was used for luciferase assays using a Dual-Luciferase Reporter Assay System. The Relative Light Unit (RLU) was determined by dividing the reading from the PGL2/ Star promoter by the reading from Renilla luciferase. The promoter activities were expressed as fold over RLU of control. ***, p
Figure Legend Snippet: Blocking Ca 2+ influx through L-type Ca 2+ channel enhanced cAMP-induced Star gene transcription in MA-10 mouse Leydig cells. MA-10 cells were cultured in serum-free Waymouth’s medium with increasing concentrations of the selective L-type Ca 2+ channel blocker, nifedipine, for 30 min and then 0.05 mM dbcAMP was added to the culture for 6 h. A, the cells were collected for total RNA isolation and Star mRNA was analyzed by RT-PCR with β-actin as an internal marker. B, MA-10 cells were transfected with a Star promoter/luciferase plasmid (PGL2/ Star ) and a pRLSV40 vector, a plasmid that constitutively expresses Renilla luciferase. The cells were then treated as described above and the cell lysate was used for luciferase assays using a Dual-Luciferase Reporter Assay System. The Relative Light Unit (RLU) was determined by dividing the reading from the PGL2/ Star promoter by the reading from Renilla luciferase. The promoter activities were expressed as fold over RLU of control. ***, p

Techniques Used: Blocking Assay, Cell Culture, Isolation, Reverse Transcription Polymerase Chain Reaction, Marker, Transfection, Luciferase, Plasmid Preparation, Reporter Assay

Synergistic interaction between nifedipine and cAMP in steroidogenesis of MA-10 mouse Leydig cells. MA-10 cells were cultured for 30 min in serum-free Waymouth’s medium with or without 20 μM of nifedipine, and then increasing concentrations of dbcAMP were added to the cultures for 6 h. A, the cells were collected and 20 μg of cell lysate protein was used for Western blot analysis of StAR protein. B, progesterone production in the culture medium was assessed by RIA. *, p
Figure Legend Snippet: Synergistic interaction between nifedipine and cAMP in steroidogenesis of MA-10 mouse Leydig cells. MA-10 cells were cultured for 30 min in serum-free Waymouth’s medium with or without 20 μM of nifedipine, and then increasing concentrations of dbcAMP were added to the cultures for 6 h. A, the cells were collected and 20 μg of cell lysate protein was used for Western blot analysis of StAR protein. B, progesterone production in the culture medium was assessed by RIA. *, p

Techniques Used: Cell Culture, Western Blot

3) Product Images from "Stable EET urea agonist and soluble epoxide hydrolase inhibitor regulate rat pulmonary arteries through TRPCs"

Article Title: Stable EET urea agonist and soluble epoxide hydrolase inhibitor regulate rat pulmonary arteries through TRPCs

Journal: Hypertension research : official journal of the Japanese Society of Hypertension

doi: 10.1038/hr.2011.5

Contribution of TRPCs and not VDCCs to increases in intracellular Ca 2+ induced by 8-HUDE. (a) Average [Ca 2+ ] i response in the absence (n=17) and presence (n¼16) of SKF or nifedipine (50 or 5 mM, respectively, pretreatment for 20 min). (b) SKF
Figure Legend Snippet: Contribution of TRPCs and not VDCCs to increases in intracellular Ca 2+ induced by 8-HUDE. (a) Average [Ca 2+ ] i response in the absence (n=17) and presence (n¼16) of SKF or nifedipine (50 or 5 mM, respectively, pretreatment for 20 min). (b) SKF

Techniques Used:

Related Articles

other:

Article Title: Induction of a novel cation current in cardiac ventricular myocytes by flufenamic acid and related drugs
Article Snippet: Oleoyl-acetyl-glycerol (OAG) and nifedipine were also from Biomol.

Article Title: Unbiased Cell-based Screening in a Neuronal Cell Model of Batten Disease Highlights an Interaction between Ca2+ Homeostasis, Autophagy, and CLN3 Protein Function *
Article Snippet: The following compounds were used: thapsigargin (Enzo catalog no. BML-PE180); BAPTA-AM (Life Technologies, Inc., catalog no. B-6769); bafilomycin B1 (AG Scientific Inc., catalog no. B-1185); tunicamycin (Sigma, catalog no. T7765); chelerythrine (Sigma, catalog no. C-2932); arvanil (Biomol catalog no. VR-101); nifedipine (Biomol catalog no. CA-210); A-23187 (Biomol catalog no. CA-100); ikarugamycin (Biomol catalog no. EI-313); and CA-074 (Biomol catalog no. PI-126).

Article Title: Upregulation of Voltage-Gated Calcium Channel Cav1.3 in Bovine Somatotropes Treated with Ghrelin
Article Snippet: Chemicals Ghrelin (ALX-157-022-M001), tetrodotoxin (BML-NA120-0001), KN-62 (BML-EI230), and nifedipine (ALX-550-091-G005) were purchased from Enzo-Alexis-Biomol (ENZO Life Sciences), GHRP-6 (HOR-298-1) was purchased from ProSpec protein specialist, and D-Lys3-GHRP-6 (G-4535-5MG) was purchased from Sigma (St. Louis, MO).

Synthesized:

Article Title: Stable EET urea agonist and soluble epoxide hydrolase inhibitor regulate rat pulmonary arteries through TRPCs
Article Snippet: .. Both 8-HUDE and 8,9-EET were synthesized in the laboratory of Dr JR Falck; and nifedipine were purchased from Biomol (Plymouth Meeting, PA, USA). .. Caffeine, La3+ and cyclopiazonic acid (CPA) were obtained from Sigma- Aldrich (St Louis, MO, USA).

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    Biomol GmbH nifedipine
    High voltage activation calcium channels' L-type regulation and pathway signaling Ca 2+ /CaM-K II affect GH release in bovine somatotropes. (a) Bar graph illustrating the regulation of hormone release by GHRP-6 (100 nM) applied alone or in the presence of <t>nifedipine</t> (0.5 μ M) a blocker of HVACC L-type. (b) Average amount of GH released in the absences (control) and after treatment with the signaling pathway Ca 2+ /CaM-K II inhibitor KN-62 (10 μ M) alone or in combination with GHRP-6 (100 nM). Each value represents the mean ± SE of determinations performed in triplicate from three independent experiments. The asterisks denote significant differences ( P
    Nifedipine, supplied by Biomol GmbH, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nifedipine/product/Biomol GmbH
    Average 92 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    nifedipine - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

    Image Search Results


    High voltage activation calcium channels' L-type regulation and pathway signaling Ca 2+ /CaM-K II affect GH release in bovine somatotropes. (a) Bar graph illustrating the regulation of hormone release by GHRP-6 (100 nM) applied alone or in the presence of nifedipine (0.5 μ M) a blocker of HVACC L-type. (b) Average amount of GH released in the absences (control) and after treatment with the signaling pathway Ca 2+ /CaM-K II inhibitor KN-62 (10 μ M) alone or in combination with GHRP-6 (100 nM). Each value represents the mean ± SE of determinations performed in triplicate from three independent experiments. The asterisks denote significant differences ( P

    Journal: Journal of Signal Transduction

    Article Title: Upregulation of Voltage-Gated Calcium Channel Cav1.3 in Bovine Somatotropes Treated with Ghrelin

    doi: 10.1155/2013/527253

    Figure Lengend Snippet: High voltage activation calcium channels' L-type regulation and pathway signaling Ca 2+ /CaM-K II affect GH release in bovine somatotropes. (a) Bar graph illustrating the regulation of hormone release by GHRP-6 (100 nM) applied alone or in the presence of nifedipine (0.5 μ M) a blocker of HVACC L-type. (b) Average amount of GH released in the absences (control) and after treatment with the signaling pathway Ca 2+ /CaM-K II inhibitor KN-62 (10 μ M) alone or in combination with GHRP-6 (100 nM). Each value represents the mean ± SE of determinations performed in triplicate from three independent experiments. The asterisks denote significant differences ( P

    Article Snippet: Chemicals Ghrelin (ALX-157-022-M001), tetrodotoxin (BML-NA120-0001), KN-62 (BML-EI230), and nifedipine (ALX-550-091-G005) were purchased from Enzo-Alexis-Biomol (ENZO Life Sciences), GHRP-6 (HOR-298-1) was purchased from ProSpec protein specialist, and D-Lys3-GHRP-6 (G-4535-5MG) was purchased from Sigma (St. Louis, MO).

    Techniques: Activation Assay, Chick Chorioallantoic Membrane Assay

    Blocking Ca 2+ influx through L-type Ca 2+ channel enhanced cAMP-induced steroidogenesis and StAR protein expression in MA-10 mouse Leydig cells. MA-10 cells were cultured in serum-free Waymouth’s medium with increasing concentrations of the selective L-type Ca 2+ channel blocker, nifedipine, for 30 min and then 0.05 mM dbcAMP was added to the culture for 6 h. A , the cells were collected and 20μg of cell lysate protein was used for Western blot analysis of StAR protein. B , progesterone concentration in the medium was assessed by RIA. **, p

    Journal: The Journal of endocrinology

    Article Title: Blocking L-type Calcium Channels Reduced the Threshold of Cyclic AMP-induced Steroidogenic Acute Regulatory Gene Expression in MA-10 Mouse Leydig Cells

    doi: 10.1677/JOE-09-0206

    Figure Lengend Snippet: Blocking Ca 2+ influx through L-type Ca 2+ channel enhanced cAMP-induced steroidogenesis and StAR protein expression in MA-10 mouse Leydig cells. MA-10 cells were cultured in serum-free Waymouth’s medium with increasing concentrations of the selective L-type Ca 2+ channel blocker, nifedipine, for 30 min and then 0.05 mM dbcAMP was added to the culture for 6 h. A , the cells were collected and 20μg of cell lysate protein was used for Western blot analysis of StAR protein. B , progesterone concentration in the medium was assessed by RIA. **, p

    Article Snippet: Nifedipine, verapamil, isadipine and diltiazem were purchased from BIOMOL (Plymouth Meeting, PA).

    Techniques: Blocking Assay, Expressing, Cell Culture, Western Blot, Concentration Assay

    Blocking Ca 2+ influx through L-type Ca 2+ channel enhanced cAMP-induced Star gene transcription in MA-10 mouse Leydig cells. MA-10 cells were cultured in serum-free Waymouth’s medium with increasing concentrations of the selective L-type Ca 2+ channel blocker, nifedipine, for 30 min and then 0.05 mM dbcAMP was added to the culture for 6 h. A, the cells were collected for total RNA isolation and Star mRNA was analyzed by RT-PCR with β-actin as an internal marker. B, MA-10 cells were transfected with a Star promoter/luciferase plasmid (PGL2/ Star ) and a pRLSV40 vector, a plasmid that constitutively expresses Renilla luciferase. The cells were then treated as described above and the cell lysate was used for luciferase assays using a Dual-Luciferase Reporter Assay System. The Relative Light Unit (RLU) was determined by dividing the reading from the PGL2/ Star promoter by the reading from Renilla luciferase. The promoter activities were expressed as fold over RLU of control. ***, p

    Journal: The Journal of endocrinology

    Article Title: Blocking L-type Calcium Channels Reduced the Threshold of Cyclic AMP-induced Steroidogenic Acute Regulatory Gene Expression in MA-10 Mouse Leydig Cells

    doi: 10.1677/JOE-09-0206

    Figure Lengend Snippet: Blocking Ca 2+ influx through L-type Ca 2+ channel enhanced cAMP-induced Star gene transcription in MA-10 mouse Leydig cells. MA-10 cells were cultured in serum-free Waymouth’s medium with increasing concentrations of the selective L-type Ca 2+ channel blocker, nifedipine, for 30 min and then 0.05 mM dbcAMP was added to the culture for 6 h. A, the cells were collected for total RNA isolation and Star mRNA was analyzed by RT-PCR with β-actin as an internal marker. B, MA-10 cells were transfected with a Star promoter/luciferase plasmid (PGL2/ Star ) and a pRLSV40 vector, a plasmid that constitutively expresses Renilla luciferase. The cells were then treated as described above and the cell lysate was used for luciferase assays using a Dual-Luciferase Reporter Assay System. The Relative Light Unit (RLU) was determined by dividing the reading from the PGL2/ Star promoter by the reading from Renilla luciferase. The promoter activities were expressed as fold over RLU of control. ***, p

    Article Snippet: Nifedipine, verapamil, isadipine and diltiazem were purchased from BIOMOL (Plymouth Meeting, PA).

    Techniques: Blocking Assay, Cell Culture, Isolation, Reverse Transcription Polymerase Chain Reaction, Marker, Transfection, Luciferase, Plasmid Preparation, Reporter Assay

    Synergistic interaction between nifedipine and cAMP in steroidogenesis of MA-10 mouse Leydig cells. MA-10 cells were cultured for 30 min in serum-free Waymouth’s medium with or without 20 μM of nifedipine, and then increasing concentrations of dbcAMP were added to the cultures for 6 h. A, the cells were collected and 20 μg of cell lysate protein was used for Western blot analysis of StAR protein. B, progesterone production in the culture medium was assessed by RIA. *, p

    Journal: The Journal of endocrinology

    Article Title: Blocking L-type Calcium Channels Reduced the Threshold of Cyclic AMP-induced Steroidogenic Acute Regulatory Gene Expression in MA-10 Mouse Leydig Cells

    doi: 10.1677/JOE-09-0206

    Figure Lengend Snippet: Synergistic interaction between nifedipine and cAMP in steroidogenesis of MA-10 mouse Leydig cells. MA-10 cells were cultured for 30 min in serum-free Waymouth’s medium with or without 20 μM of nifedipine, and then increasing concentrations of dbcAMP were added to the cultures for 6 h. A, the cells were collected and 20 μg of cell lysate protein was used for Western blot analysis of StAR protein. B, progesterone production in the culture medium was assessed by RIA. *, p

    Article Snippet: Nifedipine, verapamil, isadipine and diltiazem were purchased from BIOMOL (Plymouth Meeting, PA).

    Techniques: Cell Culture, Western Blot

    Contribution of TRPCs and not VDCCs to increases in intracellular Ca 2+ induced by 8-HUDE. (a) Average [Ca 2+ ] i response in the absence (n=17) and presence (n¼16) of SKF or nifedipine (50 or 5 mM, respectively, pretreatment for 20 min). (b) SKF

    Journal: Hypertension research : official journal of the Japanese Society of Hypertension

    Article Title: Stable EET urea agonist and soluble epoxide hydrolase inhibitor regulate rat pulmonary arteries through TRPCs

    doi: 10.1038/hr.2011.5

    Figure Lengend Snippet: Contribution of TRPCs and not VDCCs to increases in intracellular Ca 2+ induced by 8-HUDE. (a) Average [Ca 2+ ] i response in the absence (n=17) and presence (n¼16) of SKF or nifedipine (50 or 5 mM, respectively, pretreatment for 20 min). (b) SKF

    Article Snippet: Both 8-HUDE and 8,9-EET were synthesized in the laboratory of Dr JR Falck; and nifedipine were purchased from Biomol (Plymouth Meeting, PA, USA).

    Techniques: