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Addgene inc nickase cas9d10a
Nickase Cas9d10a, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/nickase cas9d10a/product/Addgene inc
Average 90 stars, based on 1 article reviews
Price from $9.99 to $1999.99
nickase cas9d10a - by Bioz Stars, 2020-04
90/100 stars

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human codon-optimized cas9
cas9
sgrna constructs

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Construct:

Article Title: Knock-in of large reporter genes in human cells via CRISPR/Cas9-induced homology-dependent and independent DNA repair
Article Snippet: .. Cas9 and sgRNA constructs The human codon-optimized Cas9 (Addgene # 41815) and nickase Cas9D10A (Addgene # 41816) plasmids were obtained from Addgene ( ). sgRNAs were designed and constructed as described previously ( , ). ..

CRISPR:

Article Title: Knock-in of large reporter genes in human cells via CRISPR/Cas9-induced homology-dependent and independent DNA repair
Article Snippet: Cas9 and sgRNA constructs The human codon-optimized Cas9 (Addgene # 41815) and nickase Cas9D10A (Addgene # 41816) plasmids were obtained from Addgene ( ). sgRNAs were designed and constructed as described previously ( , ). .. Potential off-target effects of sgRNA candidates were analyzed using the online tool CRISPR Design developed by Zhang's laboratory ( http://crispr.mit.edu/ ), and the sgRNA sequences with fewer off-target sites in human genome were selected for further analysis.

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  • 94
    Addgene inc cas9 nickase
    Generation of FRT knock-in hPSC lines using <t>Cas9</t> <t>nickase</t>
    Cas9 Nickase, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Addgene inc pspcas9n bb 2a puro
    Generation of FRT knock-in hPSC lines using <t>Cas9</t> <t>nickase</t>
    Pspcas9n Bb 2a Puro, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 95 stars, based on 18 article reviews
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    pspcas9n bb 2a puro - by Bioz Stars, 2020-04
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    90
    Addgene inc nickase cas9d10a
    Generation of FRT knock-in hPSC lines using <t>Cas9</t> <t>nickase</t>
    Nickase Cas9d10a, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nickase cas9d10a/product/Addgene inc
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    nickase cas9d10a - by Bioz Stars, 2020-04
    90/100 stars
      Buy from Supplier

    Image Search Results


    Generation of FRT knock-in hPSC lines using Cas9 nickase

    Journal: Cell stem cell

    Article Title: Engineering Human Stem Cell Lines with Inducible Gene Knockout using CRISPR/Cas9

    doi: 10.1016/j.stem.2015.06.001

    Figure Lengend Snippet: Generation of FRT knock-in hPSC lines using Cas9 nickase

    Article Snippet: Human codon-optimized Streptococcus pyogenes wild-type Cas9 (Cas9-2A-GFP) and Cas9 nickase (Cas9D10A-2A-GFP) were obtained from Addgene (plasmid #44719 and plasmid #44720) ( ).

    Techniques: Knock-In