ni nta agarose resin  (Qiagen)


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    Structured Review

    Qiagen ni nta agarose resin
    Ni Nta Agarose Resin, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 175 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ni nta agarose resin/product/Qiagen
    Average 99 stars, based on 175 article reviews
    Price from $9.99 to $1999.99
    ni nta agarose resin - by Bioz Stars, 2020-04
    99/100 stars

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    Related Articles

    Protein Purification:

    Article Title: Enhancing Terminal Deoxynucleotidyl Transferase Activity on Substrates with 3' Terminal Structures for Enzymatic De Novo DNA Synthesis.
    Article Snippet: All protein purification steps were performed at 4 ◦C. .. First, gravity columns were packed with 2 mL Ni-NTA agarose resin (Qiagen; Germantown, MD, USA) and equilibrated with 10 mL Buffer A (20 mM Tris-HCl, 0.5 M NaCl, pH 8.3) plus 5 mM imidazole.

    Incubation:

    Article Title: Enhancing Terminal Deoxynucleotidyl Transferase Activity on Substrates with 3' Terminal Structures for Enzymatic De Novo DNA Synthesis.
    Article Snippet: Expression cultures were grown to an OD600 of 0.8 at 37 ◦C and then transferred to an incubator at 15 ◦C for a 30-min incubation without shaking. .. First, gravity columns were packed with 2 mL Ni-NTA agarose resin (Qiagen; Germantown, MD, USA) and equilibrated with 10 mL Buffer A (20 mM Tris-HCl, 0.5 M NaCl, pH 8.3) plus 5 mM imidazole.

    Expressing:

    Article Title: Enhancing Terminal Deoxynucleotidyl Transferase Activity on Substrates with 3' Terminal Structures for Enzymatic De Novo DNA Synthesis.
    Article Snippet: Paragraph title: 2.2. Protein Expression and Purification of MBP-Fused TdT ... First, gravity columns were packed with 2 mL Ni-NTA agarose resin (Qiagen; Germantown, MD, USA) and equilibrated with 10 mL Buffer A (20 mM Tris-HCl, 0.5 M NaCl, pH 8.3) plus 5 mM imidazole.

    Purification:

    Article Title: Enhancing Terminal Deoxynucleotidyl Transferase Activity on Substrates with 3' Terminal Structures for Enzymatic De Novo DNA Synthesis.
    Article Snippet: Paragraph title: 2.2. Protein Expression and Purification of MBP-Fused TdT ... First, gravity columns were packed with 2 mL Ni-NTA agarose resin (Qiagen; Germantown, MD, USA) and equilibrated with 10 mL Buffer A (20 mM Tris-HCl, 0.5 M NaCl, pH 8.3) plus 5 mM imidazole.

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  • 99
    Qiagen ni nta agarose resin
    UNC-43 directly binds to and phosphorylates DAF-16. ( A ) 3xHA::UNC-43 co-immunoprecipitated with 3xFLAG::DAF-16 and ( B ) vice versa from lysates of transgenic C. elegans expressing both proteins. The transgenic strains are MQD522 (co-expressing 3xHA::UNC-43 and 3xFLAG::DAF-16), MQD530 (expressing 3xHA::UNC-43), and MQD89 (expressing 3xFLAG::DAF-16). ( C ) UNC-43 can directly bind to DAF-16. Purified GST-UNC-43 but not CAM or the GST control was pulled down by <t>Ni-NTA</t> beads through its interaction with <t>6xHis-DAF-16.</t> A Coomassie gel is shown at the bottom. ( D )–( E ) In vitro kinase assays in the presence of [ 32 P]-γ-ATP, in which purified GST-UNC-43 directly phosphorylated His-tagged DAF-16 ( D ) or DAF-16 (N-F) fragments ( E ). The Coomassie-stained gel is shown below the autoradiograph. DOI: http://dx.doi.org/10.7554/eLife.00518.010
    Ni Nta Agarose Resin, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 175 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ni nta agarose resin/product/Qiagen
    Average 99 stars, based on 175 article reviews
    Price from $9.99 to $1999.99
    ni nta agarose resin - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    94
    Qiagen nickel nitrilotriacetic acid agarose resin
    Mobility Shift of TRAB1 on SDS-PAGE Caused by ABA-Induced Phosphorylation. (A) Nuclear extracts of rice suspension-cultured cells (Oc cells) treated with ABA for the indicated times (0 indicates untreated cells) were analyzed by immunoblotting with anti-TRAB1 antibody. The arrow and asterisks indicate TRAB1-specific and nonspecific bands, respectively. Although the expression of the nonspecific bands was affected by ABA, the nature of these polypeptides is unknown. (B) TRAB1-dHA/His expressed in transgenic cells treated without (−) or with (+) ABA for 30 min was recovered with <t>nickel–nitrilotriacetic</t> acid agarose resin, incubated without (−) or with (+) CIAP, and analyzed by immunoblotting with anti-HA antibody. The signals seen at the top of the +CIAP lanes are parts of bulky CIAP bands that reacted nonspecifically with the anti-HA antibody.
    Nickel Nitrilotriacetic Acid Agarose Resin, supplied by Qiagen, used in various techniques. Bioz Stars score: 94/100, based on 51 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nickel nitrilotriacetic acid agarose resin/product/Qiagen
    Average 94 stars, based on 51 article reviews
    Price from $9.99 to $1999.99
    nickel nitrilotriacetic acid agarose resin - by Bioz Stars, 2020-04
    94/100 stars
      Buy from Supplier

    95
    Qiagen ni nitrilotriacetic acid nta sepharose resin
    Targeted gene insertion and expression in E. coli . ( A ) The gel electrophoresis of amplified PCR cellulose products from B. licheniformis ATCC 14580 (lane 1) and INP from P. syringae KCTC 1832 (lane 2). M: 1 kb DNA marker; ( B ) SDS-PAGE analysis of the recombinant cells; M: standard protein size marker (molecular biomasses in kilodaltons), lane 1: the supernatant fraction of recombinant cell culture medium, lane 2: the total cell lysates of recombinant cell; ( C ) The purified fusion proteins following <t>Ni-nitrilotriacetic</t> acid <t>(NTA)-sepharose</t> resin treatment; M: standard protein size marker (kDa), lane 1: imidazole concentration of 20 mM in the binding buffer, lane 2: imidazole concentration of 50 mM in the binding buffer, lane 3: imidazole concentration of 100 mM in the binding buffer; ( D ) Western blot analysis of the purified fusion protein from SDS-PAGE results probed with anti-His-tag antibody, respectively.
    Ni Nitrilotriacetic Acid Nta Sepharose Resin, supplied by Qiagen, used in various techniques. Bioz Stars score: 95/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ni nitrilotriacetic acid nta sepharose resin/product/Qiagen
    Average 95 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    ni nitrilotriacetic acid nta sepharose resin - by Bioz Stars, 2020-04
    95/100 stars
      Buy from Supplier

    Image Search Results


    UNC-43 directly binds to and phosphorylates DAF-16. ( A ) 3xHA::UNC-43 co-immunoprecipitated with 3xFLAG::DAF-16 and ( B ) vice versa from lysates of transgenic C. elegans expressing both proteins. The transgenic strains are MQD522 (co-expressing 3xHA::UNC-43 and 3xFLAG::DAF-16), MQD530 (expressing 3xHA::UNC-43), and MQD89 (expressing 3xFLAG::DAF-16). ( C ) UNC-43 can directly bind to DAF-16. Purified GST-UNC-43 but not CAM or the GST control was pulled down by Ni-NTA beads through its interaction with 6xHis-DAF-16. A Coomassie gel is shown at the bottom. ( D )–( E ) In vitro kinase assays in the presence of [ 32 P]-γ-ATP, in which purified GST-UNC-43 directly phosphorylated His-tagged DAF-16 ( D ) or DAF-16 (N-F) fragments ( E ). The Coomassie-stained gel is shown below the autoradiograph. DOI: http://dx.doi.org/10.7554/eLife.00518.010

    Journal: eLife

    Article Title: CAMKII and Calcineurin regulate the lifespan of Caenorhabditis elegans through the FOXO transcription factor DAF-16

    doi: 10.7554/eLife.00518

    Figure Lengend Snippet: UNC-43 directly binds to and phosphorylates DAF-16. ( A ) 3xHA::UNC-43 co-immunoprecipitated with 3xFLAG::DAF-16 and ( B ) vice versa from lysates of transgenic C. elegans expressing both proteins. The transgenic strains are MQD522 (co-expressing 3xHA::UNC-43 and 3xFLAG::DAF-16), MQD530 (expressing 3xHA::UNC-43), and MQD89 (expressing 3xFLAG::DAF-16). ( C ) UNC-43 can directly bind to DAF-16. Purified GST-UNC-43 but not CAM or the GST control was pulled down by Ni-NTA beads through its interaction with 6xHis-DAF-16. A Coomassie gel is shown at the bottom. ( D )–( E ) In vitro kinase assays in the presence of [ 32 P]-γ-ATP, in which purified GST-UNC-43 directly phosphorylated His-tagged DAF-16 ( D ) or DAF-16 (N-F) fragments ( E ). The Coomassie-stained gel is shown below the autoradiograph. DOI: http://dx.doi.org/10.7554/eLife.00518.010

    Article Snippet: Recombinant 6xHis-DAF-16 and 6xHis-DAF-16-6AM were expressed in BL21 and affinity purified using Ni-NTA agarose resin (Qiagen) in buffer containing 20 mM HEPES, pH 7.5, 150 mM NaCl, and 0.5% NP-40.

    Techniques: Immunoprecipitation, Transgenic Assay, Expressing, Purification, Chick Chorioallantoic Membrane Assay, In Vitro, Staining, Autoradiography

    C. elegans Calcineurin TAX-6•CNB-1 directly binds to DAF-16 and negatively regulates DAF-16 nuclear localization. ( A ) DAF-16 and TAX-6 form a complex in vivo. Immunoprecipitation of 3xFLAG::DAF-16 expressed under the daf-16 promoter in WT C. elegans pulled down TAX-6::GFP expressed under the tax-6 promoter. The lysates were obtained from transgenic strains MQD82 (co-expressing 3xFLAG::DAF-16 and TAX-6::GFP), MQD2 (expressing TAX-6::GFP), MQD89 (expressing 3xFLAG::DAF-16), and CF1553 (expressing GFP under a sod-3 promoter). ( B ) Calcineurin directly binds to DAF-16. Purified recombinant TAX-6•GST-CNB-1 was pulled down with Ni-NTA beads through its interaction with purified His-tagged DAF-16. ( C ) The C-terminal region of DAF-16 most likely mediates the interaction with Calcineurin. GST-DAF-16(F-C), but not GST, GST-DAF-16(N) or GST-DAF-16(N-F), pulled down TAX-6::GFP expressed in C. elegans (strain MQD5). The DAF-16 C-terminal region alone was not stable. Asterisk indicates full-length GST or GST fusion proteins. ( D ) DAF-16::6xHis::GFP is diffusely distributed in the WT animals but concentrated in the nucleus in tax-6(ok2065) animals. All GFP images shown in this paper are of L4 animals at 20°C unless otherwise indicated. DOI: http://dx.doi.org/10.7554/eLife.00518.003

    Journal: eLife

    Article Title: CAMKII and Calcineurin regulate the lifespan of Caenorhabditis elegans through the FOXO transcription factor DAF-16

    doi: 10.7554/eLife.00518

    Figure Lengend Snippet: C. elegans Calcineurin TAX-6•CNB-1 directly binds to DAF-16 and negatively regulates DAF-16 nuclear localization. ( A ) DAF-16 and TAX-6 form a complex in vivo. Immunoprecipitation of 3xFLAG::DAF-16 expressed under the daf-16 promoter in WT C. elegans pulled down TAX-6::GFP expressed under the tax-6 promoter. The lysates were obtained from transgenic strains MQD82 (co-expressing 3xFLAG::DAF-16 and TAX-6::GFP), MQD2 (expressing TAX-6::GFP), MQD89 (expressing 3xFLAG::DAF-16), and CF1553 (expressing GFP under a sod-3 promoter). ( B ) Calcineurin directly binds to DAF-16. Purified recombinant TAX-6•GST-CNB-1 was pulled down with Ni-NTA beads through its interaction with purified His-tagged DAF-16. ( C ) The C-terminal region of DAF-16 most likely mediates the interaction with Calcineurin. GST-DAF-16(F-C), but not GST, GST-DAF-16(N) or GST-DAF-16(N-F), pulled down TAX-6::GFP expressed in C. elegans (strain MQD5). The DAF-16 C-terminal region alone was not stable. Asterisk indicates full-length GST or GST fusion proteins. ( D ) DAF-16::6xHis::GFP is diffusely distributed in the WT animals but concentrated in the nucleus in tax-6(ok2065) animals. All GFP images shown in this paper are of L4 animals at 20°C unless otherwise indicated. DOI: http://dx.doi.org/10.7554/eLife.00518.003

    Article Snippet: Recombinant 6xHis-DAF-16 and 6xHis-DAF-16-6AM were expressed in BL21 and affinity purified using Ni-NTA agarose resin (Qiagen) in buffer containing 20 mM HEPES, pH 7.5, 150 mM NaCl, and 0.5% NP-40.

    Techniques: In Vivo, Immunoprecipitation, Transgenic Assay, Expressing, Purification, Recombinant

    SDS-PAGE of recombinant FumF protein . Proteins were separated by 12% (w/v) SDS-PAGE and then stained with Coomassie brilliant blue G-250. Lane 1, molecular weight standards; Lane 2, total protein of E. coli BL21(DE3)pLysS harboring empty pETBlue-2 (control); Lane 3, total protein of E. coli BL21(DE3)pLysS harboring the recombinant fumF in pETBlue-2 without induction by IPTG; Lane 4, total protein of E. coli BL21(DE3)pLysS harboring the recombinant fumF in pETBlue-2 induced by addition of 0.5 mM IPTG; Lane 5, sample purified by the Ni-NTA column method. The recombinant FumF protein is indicated by the black arrow.

    Journal: Microbial Cell Factories

    Article Title: Identification and characterization of a novel fumarase gene by metagenome expression cloning from marine microorganisms

    doi: 10.1186/1475-2859-9-91

    Figure Lengend Snippet: SDS-PAGE of recombinant FumF protein . Proteins were separated by 12% (w/v) SDS-PAGE and then stained with Coomassie brilliant blue G-250. Lane 1, molecular weight standards; Lane 2, total protein of E. coli BL21(DE3)pLysS harboring empty pETBlue-2 (control); Lane 3, total protein of E. coli BL21(DE3)pLysS harboring the recombinant fumF in pETBlue-2 without induction by IPTG; Lane 4, total protein of E. coli BL21(DE3)pLysS harboring the recombinant fumF in pETBlue-2 induced by addition of 0.5 mM IPTG; Lane 5, sample purified by the Ni-NTA column method. The recombinant FumF protein is indicated by the black arrow.

    Article Snippet: The His-tagged FumF protein was expressed and purified using Ni-NTA agarose resin (Qiagen, Valencia, CA, USA), according to the manufacturer's instructions.

    Techniques: SDS Page, Recombinant, Staining, Molecular Weight, Purification

    Mobility Shift of TRAB1 on SDS-PAGE Caused by ABA-Induced Phosphorylation. (A) Nuclear extracts of rice suspension-cultured cells (Oc cells) treated with ABA for the indicated times (0 indicates untreated cells) were analyzed by immunoblotting with anti-TRAB1 antibody. The arrow and asterisks indicate TRAB1-specific and nonspecific bands, respectively. Although the expression of the nonspecific bands was affected by ABA, the nature of these polypeptides is unknown. (B) TRAB1-dHA/His expressed in transgenic cells treated without (−) or with (+) ABA for 30 min was recovered with nickel–nitrilotriacetic acid agarose resin, incubated without (−) or with (+) CIAP, and analyzed by immunoblotting with anti-HA antibody. The signals seen at the top of the +CIAP lanes are parts of bulky CIAP bands that reacted nonspecifically with the anti-HA antibody.

    Journal: The Plant Cell

    Article Title: Abscisic Acid-Induced Transcription Is Mediated by Phosphorylation of an Abscisic Acid Response Element Binding Factor, TRAB1

    doi: 10.1105/tpc.005272

    Figure Lengend Snippet: Mobility Shift of TRAB1 on SDS-PAGE Caused by ABA-Induced Phosphorylation. (A) Nuclear extracts of rice suspension-cultured cells (Oc cells) treated with ABA for the indicated times (0 indicates untreated cells) were analyzed by immunoblotting with anti-TRAB1 antibody. The arrow and asterisks indicate TRAB1-specific and nonspecific bands, respectively. Although the expression of the nonspecific bands was affected by ABA, the nature of these polypeptides is unknown. (B) TRAB1-dHA/His expressed in transgenic cells treated without (−) or with (+) ABA for 30 min was recovered with nickel–nitrilotriacetic acid agarose resin, incubated without (−) or with (+) CIAP, and analyzed by immunoblotting with anti-HA antibody. The signals seen at the top of the +CIAP lanes are parts of bulky CIAP bands that reacted nonspecifically with the anti-HA antibody.

    Article Snippet: TRAB1-dHA/His protein in the nuclear or protoplast extracts was recovered with nickel-nitrilotriacetic acid agarose resin (Qiagen).

    Techniques: Mobility Shift, SDS Page, Cell Culture, Expressing, Transgenic Assay, Incubation

    Targeted gene insertion and expression in E. coli . ( A ) The gel electrophoresis of amplified PCR cellulose products from B. licheniformis ATCC 14580 (lane 1) and INP from P. syringae KCTC 1832 (lane 2). M: 1 kb DNA marker; ( B ) SDS-PAGE analysis of the recombinant cells; M: standard protein size marker (molecular biomasses in kilodaltons), lane 1: the supernatant fraction of recombinant cell culture medium, lane 2: the total cell lysates of recombinant cell; ( C ) The purified fusion proteins following Ni-nitrilotriacetic acid (NTA)-sepharose resin treatment; M: standard protein size marker (kDa), lane 1: imidazole concentration of 20 mM in the binding buffer, lane 2: imidazole concentration of 50 mM in the binding buffer, lane 3: imidazole concentration of 100 mM in the binding buffer; ( D ) Western blot analysis of the purified fusion protein from SDS-PAGE results probed with anti-His-tag antibody, respectively.

    Journal: Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry

    Article Title: Bacillus Cellulase Molecular Cloning, Expression, and Surface Display on the Outer Membrane of Escherichia coli

    doi: 10.3390/molecules23020503

    Figure Lengend Snippet: Targeted gene insertion and expression in E. coli . ( A ) The gel electrophoresis of amplified PCR cellulose products from B. licheniformis ATCC 14580 (lane 1) and INP from P. syringae KCTC 1832 (lane 2). M: 1 kb DNA marker; ( B ) SDS-PAGE analysis of the recombinant cells; M: standard protein size marker (molecular biomasses in kilodaltons), lane 1: the supernatant fraction of recombinant cell culture medium, lane 2: the total cell lysates of recombinant cell; ( C ) The purified fusion proteins following Ni-nitrilotriacetic acid (NTA)-sepharose resin treatment; M: standard protein size marker (kDa), lane 1: imidazole concentration of 20 mM in the binding buffer, lane 2: imidazole concentration of 50 mM in the binding buffer, lane 3: imidazole concentration of 100 mM in the binding buffer; ( D ) Western blot analysis of the purified fusion protein from SDS-PAGE results probed with anti-His-tag antibody, respectively.

    Article Snippet: The (His) 6-tagged cellulase protein was bound to Ni-nitrilotriacetic acid (NTA)-sepharose resin (Qiagen, Hilden, Germany), pre-equilibrated with binding buffer, and washed with imidazole in a step-gradient manner range of 20 to 100 mM.

    Techniques: Expressing, Nucleic Acid Electrophoresis, Amplification, Polymerase Chain Reaction, Marker, SDS Page, Recombinant, Cell Culture, Purification, Concentration Assay, Binding Assay, Western Blot