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Microarray
Preparation of a mannose-6-phosphate glycan microarray through fluorescent derivatization, phosphorylation, and immobilization of natural high-mannose N-glycans and its application in ligand identification of P-type lectins
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Preparation of a mannose-6-phosphate glycan microarray through fluorescent derivatization, phosphorylation, and immobilization of natural high-mannose N-glycans and its application in ligand identification of P-type lectins
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Article Snippet
"MALDI-TOF/TOF (Bruker UltraflexII) Piezorray non-contact printer (Perkin-Elmer) ProScanArray Scanner (Perkin-Elmer) Printing buffer (2X): 600 m M sodium phosphate buffer, pH 8.5 NHS-activated microarray glass slides (Schott North America) Biotin-hydrazine (Sigma-Aldrich) Blocking buffer: 50 m M ethanolamine in 0.1 M Tris buffer, pH 9.0 384 well plate, V-shape (Biorad)"
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"Table 1The chart IDs of the individual glycans printed on the microarray. Table 1"
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