native mouse ngf 2.5s protein  (Alomone Labs)


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    Alomone Labs native mouse ngf 2.5s protein
    Native Mouse Ngf 2.5s Protein, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/native mouse ngf 2.5s protein/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    native mouse ngf 2.5s protein - by Bioz Stars, 2023-09
    94/100 stars

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    ngf  (Alomone Labs)


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    Alomone Labs ngf
    Ngf, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ngf/product/Alomone Labs
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ngf - by Bioz Stars, 2023-09
    86/100 stars

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    ngf  (Alomone Labs)


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    Alomone Labs ngf
    ( A - B ): Representative immunoblots ( A ) and densitometric quantitative analysis ( B ) showing levels of <t>NGF,</t> <t>BDNF,</t> GAP-43, and DβH, in total cardiac (top panels) and cerebral cortex (bottom panels) lysates from WT and Tg2576 mice. ( C-D ): digital images (C, scale bar 100μm) and quantifications (D) showing cardiac adrenergic nerve fibers, labeled with anti-tyrosine-hydroxylase (TH, in green), and cardiac regenerating nerve endings, labeled with anti-neuronal regeneration marker (GAP-43, in red) in heart sections from WT and Tg2576 mice. n=3-5 mice/group. Data are presented as a mean±SEM. *P<0.05, **P<0.01 and vs WT. Student t-tests have been performed between the 2 groups.
    Ngf, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ngf/product/Alomone Labs
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ngf - by Bioz Stars, 2023-09
    86/100 stars

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    1) Product Images from "Amyloid β induces cardiac dysfunction and neuro-signaling impairment in the heart of an Alzheimer’s disease model"

    Article Title: Amyloid β induces cardiac dysfunction and neuro-signaling impairment in the heart of an Alzheimer’s disease model

    Journal: bioRxiv

    doi: 10.1101/2023.07.11.548558

    ( A - B ): Representative immunoblots ( A ) and densitometric quantitative analysis ( B ) showing levels of NGF, BDNF, GAP-43, and DβH, in total cardiac (top panels) and cerebral cortex (bottom panels) lysates from WT and Tg2576 mice. ( C-D ): digital images (C, scale bar 100μm) and quantifications (D) showing cardiac adrenergic nerve fibers, labeled with anti-tyrosine-hydroxylase (TH, in green), and cardiac regenerating nerve endings, labeled with anti-neuronal regeneration marker (GAP-43, in red) in heart sections from WT and Tg2576 mice. n=3-5 mice/group. Data are presented as a mean±SEM. *P<0.05, **P<0.01 and vs WT. Student t-tests have been performed between the 2 groups.
    Figure Legend Snippet: ( A - B ): Representative immunoblots ( A ) and densitometric quantitative analysis ( B ) showing levels of NGF, BDNF, GAP-43, and DβH, in total cardiac (top panels) and cerebral cortex (bottom panels) lysates from WT and Tg2576 mice. ( C-D ): digital images (C, scale bar 100μm) and quantifications (D) showing cardiac adrenergic nerve fibers, labeled with anti-tyrosine-hydroxylase (TH, in green), and cardiac regenerating nerve endings, labeled with anti-neuronal regeneration marker (GAP-43, in red) in heart sections from WT and Tg2576 mice. n=3-5 mice/group. Data are presented as a mean±SEM. *P<0.05, **P<0.01 and vs WT. Student t-tests have been performed between the 2 groups.

    Techniques Used: Western Blot, Labeling, Marker

    Representative immunoblots ( A and C ) and densitometric quantitative analysis ( B and D ) showing protein levels of BDNF, NGF, and GAP-43 ( B - D ) in total protein lysates from human neuroblastoma cells (SH-SY5Y) stimulated with human Aβ40 (left) or Aβ42 (right) oligomers for 16 h. GAPDH levels were used as a loading control. n=9 biological replicates. Data are presented as a mean±SEM. *P<0.05, **P<0.01, ***P<0.001 and vs WT. Student t-tests have been performed between the 2 groups. (NT= Not Treated).
    Figure Legend Snippet: Representative immunoblots ( A and C ) and densitometric quantitative analysis ( B and D ) showing protein levels of BDNF, NGF, and GAP-43 ( B - D ) in total protein lysates from human neuroblastoma cells (SH-SY5Y) stimulated with human Aβ40 (left) or Aβ42 (right) oligomers for 16 h. GAPDH levels were used as a loading control. n=9 biological replicates. Data are presented as a mean±SEM. *P<0.05, **P<0.01, ***P<0.001 and vs WT. Student t-tests have been performed between the 2 groups. (NT= Not Treated).

    Techniques Used: Western Blot

    ( A - C ): Representative immunoblots ( A ) and densitometric quantitative analysis ( B - C ) showing protein levels of BDNF ( B ), and NGF ( C ) in total protein lysates from human cardiomyocytes (AC16) stimulated with human Aβ-40 oligomers for 16 h. GAPDH levels were used as a loading control. n= 6-7 biological replicates. Data are presented as a mean±SEM. **P<0.01 and vs WT. Student t-tests have been performed between the groups. (NT= Not Treated).
    Figure Legend Snippet: ( A - C ): Representative immunoblots ( A ) and densitometric quantitative analysis ( B - C ) showing protein levels of BDNF ( B ), and NGF ( C ) in total protein lysates from human cardiomyocytes (AC16) stimulated with human Aβ-40 oligomers for 16 h. GAPDH levels were used as a loading control. n= 6-7 biological replicates. Data are presented as a mean±SEM. **P<0.01 and vs WT. Student t-tests have been performed between the groups. (NT= Not Treated).

    Techniques Used: Western Blot

    ngf  (Alomone Labs)


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    Alomone Labs ngf
    Ngf, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ngf/product/Alomone Labs
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ngf - by Bioz Stars, 2023-09
    86/100 stars

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    ngf  (Alomone Labs)


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    Alomone Labs ngf
    JAK1 palmitoylation by ZDHHC3/7 is important for DRG neuron signaling and survival in response to neuropoietic cytokines. A , Western blots of lysates of DRG neurons that had been cultured in the continued presence of <t>NGF</t> to ensure survival, infected with the indicated lentiviruses and subsequently stimulated <t>with</t> <t>LIF.</t> B , quantified data from A confirm robust LIF-induced STAT3 phosphorylation, which is prevented by Jak1 knockdown and rescued by JAK1WT∗ but not JAK1CCSS∗. ∗∗∗∗ p < 0.0001; ∗∗∗ p < 0.001; ∗∗ p < 0.01; ∗ p < 0.05, one-way ANOVA with Tukey test. N = 3 per condition. F (3, 8) = 33.91, p < 0.0001. C , images of cultured DRG neurons immunostained with the indicated antibodies 4 days after being plated in the presence of LIF and infected with lentiviruses to express GFP plus a scrambled shRNA (Scrbld sh; top row ), GFP plus Jak1 shRNA (Jak1sh; second row), GFP, JAK1 shRNA and shRNA-resistant JAK1WT (JAK1WT∗; third row) or GFP, JAK1 shRNA and shRNA-resistant JAK1CCSS (JAK1CCSS∗; fourth row). Scale bar: 100 μm. D , quantified data from C confirm greatly reduced numbers of GFP + /NeuN + neurons after Jak1 knockdown, an effect rescued by JAK1WT∗ but not JAK1CCSS∗. ∗∗∗ p < 0.001, ∗∗ p < 0.01, one-way ANOVA with Dunnett post hoc test. N = 3 per condition. F (3, 8) = 19.19, p = 0.0005. E , Western blots from control (expressing scrambled sh; Scrbld sh) or Zdhhc3/7 knockdown DRG neurons stimulated with LIF or left unstimulated and immunoblotted with the indicated antibodies. F , quantified data from E confirm reduced LIF-induced STAT3 phosphorylation in Zdhhc3/ 7 knockdown neurons. ∗∗∗ p < 0.001, two-way ANOVA with Tukey test. N = 4 per condition. Virus condition p < 0.001 ( F (1, 14) = 17.28), treatment p < 0.0001 ( F (1, 14) = 122.8), interaction p = 0.0012, ( F (1, 14) = 16.34).
    Ngf, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ngf/product/Alomone Labs
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ngf - by Bioz Stars, 2023-09
    86/100 stars

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    1) Product Images from "Palmitoylation-dependent control of JAK1 kinase signaling governs responses to neuropoietic cytokines and survival in DRG neurons"

    Article Title: Palmitoylation-dependent control of JAK1 kinase signaling governs responses to neuropoietic cytokines and survival in DRG neurons

    Journal: The Journal of Biological Chemistry

    doi: 10.1016/j.jbc.2023.104965

    JAK1 palmitoylation by ZDHHC3/7 is important for DRG neuron signaling and survival in response to neuropoietic cytokines. A , Western blots of lysates of DRG neurons that had been cultured in the continued presence of NGF to ensure survival, infected with the indicated lentiviruses and subsequently stimulated with LIF. B , quantified data from A confirm robust LIF-induced STAT3 phosphorylation, which is prevented by Jak1 knockdown and rescued by JAK1WT∗ but not JAK1CCSS∗. ∗∗∗∗ p < 0.0001; ∗∗∗ p < 0.001; ∗∗ p < 0.01; ∗ p < 0.05, one-way ANOVA with Tukey test. N = 3 per condition. F (3, 8) = 33.91, p < 0.0001. C , images of cultured DRG neurons immunostained with the indicated antibodies 4 days after being plated in the presence of LIF and infected with lentiviruses to express GFP plus a scrambled shRNA (Scrbld sh; top row ), GFP plus Jak1 shRNA (Jak1sh; second row), GFP, JAK1 shRNA and shRNA-resistant JAK1WT (JAK1WT∗; third row) or GFP, JAK1 shRNA and shRNA-resistant JAK1CCSS (JAK1CCSS∗; fourth row). Scale bar: 100 μm. D , quantified data from C confirm greatly reduced numbers of GFP + /NeuN + neurons after Jak1 knockdown, an effect rescued by JAK1WT∗ but not JAK1CCSS∗. ∗∗∗ p < 0.001, ∗∗ p < 0.01, one-way ANOVA with Dunnett post hoc test. N = 3 per condition. F (3, 8) = 19.19, p = 0.0005. E , Western blots from control (expressing scrambled sh; Scrbld sh) or Zdhhc3/7 knockdown DRG neurons stimulated with LIF or left unstimulated and immunoblotted with the indicated antibodies. F , quantified data from E confirm reduced LIF-induced STAT3 phosphorylation in Zdhhc3/ 7 knockdown neurons. ∗∗∗ p < 0.001, two-way ANOVA with Tukey test. N = 4 per condition. Virus condition p < 0.001 ( F (1, 14) = 17.28), treatment p < 0.0001 ( F (1, 14) = 122.8), interaction p = 0.0012, ( F (1, 14) = 16.34).
    Figure Legend Snippet: JAK1 palmitoylation by ZDHHC3/7 is important for DRG neuron signaling and survival in response to neuropoietic cytokines. A , Western blots of lysates of DRG neurons that had been cultured in the continued presence of NGF to ensure survival, infected with the indicated lentiviruses and subsequently stimulated with LIF. B , quantified data from A confirm robust LIF-induced STAT3 phosphorylation, which is prevented by Jak1 knockdown and rescued by JAK1WT∗ but not JAK1CCSS∗. ∗∗∗∗ p < 0.0001; ∗∗∗ p < 0.001; ∗∗ p < 0.01; ∗ p < 0.05, one-way ANOVA with Tukey test. N = 3 per condition. F (3, 8) = 33.91, p < 0.0001. C , images of cultured DRG neurons immunostained with the indicated antibodies 4 days after being plated in the presence of LIF and infected with lentiviruses to express GFP plus a scrambled shRNA (Scrbld sh; top row ), GFP plus Jak1 shRNA (Jak1sh; second row), GFP, JAK1 shRNA and shRNA-resistant JAK1WT (JAK1WT∗; third row) or GFP, JAK1 shRNA and shRNA-resistant JAK1CCSS (JAK1CCSS∗; fourth row). Scale bar: 100 μm. D , quantified data from C confirm greatly reduced numbers of GFP + /NeuN + neurons after Jak1 knockdown, an effect rescued by JAK1WT∗ but not JAK1CCSS∗. ∗∗∗ p < 0.001, ∗∗ p < 0.01, one-way ANOVA with Dunnett post hoc test. N = 3 per condition. F (3, 8) = 19.19, p = 0.0005. E , Western blots from control (expressing scrambled sh; Scrbld sh) or Zdhhc3/7 knockdown DRG neurons stimulated with LIF or left unstimulated and immunoblotted with the indicated antibodies. F , quantified data from E confirm reduced LIF-induced STAT3 phosphorylation in Zdhhc3/ 7 knockdown neurons. ∗∗∗ p < 0.001, two-way ANOVA with Tukey test. N = 4 per condition. Virus condition p < 0.001 ( F (1, 14) = 17.28), treatment p < 0.0001 ( F (1, 14) = 122.8), interaction p = 0.0012, ( F (1, 14) = 16.34).

    Techniques Used: Western Blot, Cell Culture, Infection, shRNA, Expressing

    mouse ngf  (Alomone Labs)


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    Alomone Labs mouse ngf
    Mouse Ngf, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 86 stars, based on 1 article reviews
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    ngf  (Alomone Labs)


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    Alomone Labs ngf
    Ngf, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ngf/product/Alomone Labs
    Average 86 stars, based on 1 article reviews
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    ngf  (Alomone Labs)


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    Alomone Labs ngf
    Ngf, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ngf/product/Alomone Labs
    Average 86 stars, based on 1 article reviews
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    native mouse ngf 2.5s protein  (Alomone Labs)


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    Alomone Labs native mouse ngf 2.5s protein
    Native Mouse Ngf 2.5s Protein, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/native mouse ngf 2.5s protein/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
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    native mouse ngf 2 5  (Alomone Labs)


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    Alomone Labs native mouse ngf 2 5
    Native Mouse Ngf 2 5, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/native mouse ngf 2 5/product/Alomone Labs
    Average 86 stars, based on 1 article reviews
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    native mouse ngf 2.5s protein  (Alomone Labs)


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    Alomone Labs native mouse ngf 2.5s protein
    Native Mouse Ngf 2.5s Protein, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/native mouse ngf 2.5s protein/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    native mouse ngf 2.5s protein - by Bioz Stars, 2023-09
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    Alomone Labs native mouse ngf 2.5s protein
    Native Mouse Ngf 2.5s Protein, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ngf  (Alomone Labs)
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    Alomone Labs ngf
    Ngf, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    mouse ngf  (Alomone Labs)
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    Alomone Labs mouse ngf
    Mouse Ngf, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    native mouse ngf 2 5  (Alomone Labs)
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    Alomone Labs native mouse ngf 2 5
    Native Mouse Ngf 2 5, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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