Ngf, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
1) Product Images from "Neurotrophins regulate ApoER2 proteolysis through activation of the Trk signaling pathway"
Article Title: Neurotrophins regulate ApoER2 proteolysis through activation of the Trk signaling pathway
Journal: BMC Neuroscience
Figure Legend Snippet: PI3K activity regulates the constitutive levels of ApoER2 CTF but is not involved in ApoER2 shedding induced by NGF. (A) PC12-ApoER2 cells were serum-starved and pre-treated with 10 μM DAPT and 50 μM LY294002 or 5 μM ZSTK474 for 1 h. Then, the cells were incubated with 100 ng/mL NGF for 2 h. ApoER2 and p75 NTR were recognized using antibodies directed against their intracellular regions. The activation of PI3K, induced by NGF, was determined by detection of phospho-AKT. α-tubulin is shown as a loading control. The blot levels of ApoER2 CTF (B and C) and of p75 NTR CTF (D and E) were normalized to the loading control α-tubulin and plotted as the average ± SD of three independent experiments. One way ANOVA, Holm-Sidak post-hoc test, * P
Techniques Used: Activity Assay, Incubation, Activation Assay
2) Product Images from "Regulation of BDNF Release by ARMS/Kidins220 through Modulation of Synaptotagmin-IV Levels"
Article Title: Regulation of BDNF Release by ARMS/Kidins220 through Modulation of Synaptotagmin-IV Levels
Journal: The Journal of Neuroscience
Figure Legend Snippet: ARMS regulates BDNF release in DRG and cortical neurons. A , ARMS depletion in cultured DRG neurons. Cultured DRG neurons were infected at DIV 4 with control (shControl), ARMS shRNA-1 (shARMS-1), or ARMS shRNA-2 (shARMS-2) lentiviruses and lysates were obtained at DIV 11. Western blot analyses were performed. A representative blot is shown ( n = 4). B , BDNF secretion in response to NGF is enhanced in ARMS-depleted DRG neurons. BDNF ELISA was performed using the supernatant of DRG neurons from A that were nonstimulated (basal) and then stimulated with NGF for 30 min at DIV 11. Cell lysates were collected to assess BDNF levels ( n = 4). Paired Student's t test, mean ± SEM. shControl versus shARMS-1, t = 3.745, df = 3; shControl versus shARMS-2, t = 3.638, df = 3. C , ARMS knockdown in cultured cortical neurons. Cultured cortical neurons were infected with shControl, shARMS-1, or shARMS-2 lentiviruses at DIV 2 and with lentiviruses expressing BDNF at DIV 7. Cell lysates were obtained at DIV 10. Western blot analyses were performed. A representative blot is shown ( n = 4). D , ARMS knockdown potentiates BDNF release in response to different stimuli in cortical neurons. BDNF ELISA was performed using the supernatant of cortical neurons from C that were nonstimulated (basal) and then stimulated with KCl, NT-3, or NT-4 for 30 min at DIV 10 ( n = 9, n = 5, and n = 4 for shControl, shARMS-1, and shARMS-2, respectively). Unpaired Student's t test, mean ± SEM. K + Depol: shControl versus shARMS-1, t = 2.846, df = 12; shControl versus shARMS-2, t = 5.923, df = 11; NT-3: shControl versus shARMS-1, t = 3.917, df = 12; shControl versus shARMS-2, t = 10.03, df = 11; NT-4: shControl versus shARMS-1, t = 3.742, df = 12; shControl versus shARMS-2, t .
Techniques Used: Cell Culture, Infection, shRNA, Western Blot, Enzyme-linked Immunosorbent Assay, Expressing
3) Product Images from "TrkA In Vivo Function Is Negatively Regulated by Ubiquitination"
Article Title: TrkA In Vivo Function Is Negatively Regulated by Ubiquitination
Journal: The Journal of Neuroscience
Figure Legend Snippet: NGF leads to increased TrkA activation and higher AKT and MEK phosphorylation in TrkAΔKFG mutant neurons. A , DRG neurons were isolated from E13.5 WT (+/+), heterozygous (+/−), or TrkAΔKFG mutant (−/−) embryos (6–8
Techniques Used: Activation Assay, Mutagenesis, Isolation
Figure Legend Snippet: Lys 450 in the KFG motif is important for the ubiquitination of TrkA receptors in response to NGF. A–C , HEK293T cell lines stably expressing a single copy of WT, KFG deleted (ΔKFG), AFG (single lysine to alanine mutation), or KAA (phenylalanine
Techniques Used: Stable Transfection, Expressing, Mutagenesis
Figure Legend Snippet: Deletion of the KFG domain from TrkA alters NGF-induced trafficking of TrkA. A , HEK293T cells stably expressing WT or KFG deleted TrkA as in were treated with 100 ng/ml NGF for the indicated time (1–6 h) followed by biotinylation. Cell
Techniques Used: Stable Transfection, Expressing
4) Product Images from "B-RAF kinase drives developmental axon growth and promotes axon regeneration in the injured mature CNS"
Article Title: B-RAF kinase drives developmental axon growth and promotes axon regeneration in the injured mature CNS
Journal: The Journal of Experimental Medicine
Figure Legend Snippet: Expression of kaB-RAF substantially rescues sensory afferent growth in the absence of TrkA/NGF signaling. (A, left) Normal sensory cutaneous innervation at E16.5. (middle) Sensory cutaneous innervation is lost in embryos lacking the NGF receptor TrkA. (right) Expression of kaB-RAF restores cutaneous innervation. Arrowheads label the blue β-gal–positive (presumptive TrkA + ) sensory trajectories. (B) Visualization of axon growth patterns after tissue clearing. The thoracic somatosensory innervation driven by kaB-RAF in a TrkA −/− embryo (bottom; compare with middle for TrkA −/− alone) is similar to that seen in a control TrkA WT/− littermate (top). White arrowheads indicate the normal pathways of peripheral axons extending from thoracic DRGs. Red arrowheads indicate sensory projections rescued by kaB-RAF in the TrkA −/− background. (C) Expression of kaB-RAF substantially rescues trigeminal TrkA + afferent growth in the absence of TrkA/NGF signaling. Presumptive TrkA + trigeminal axon projections (top) are lost in TrkA-deficient mice (middle) and are rescued by kaB-RAF (bottom). Ga, great auricular nerve; Go, greater occipital nerve; Mn, mandibular branch; Mx, maxillary branch; Op, ophthalmical branch. Images show littermates and are representative of three embryos per genotype. Bars: (A) 2 mm; (B and C) 1 mm.
Techniques Used: Expressing, Mouse Assay
Figure Legend Snippet: Activation of B-RAF indirectly rescues CGRP expression in TrkA −/− nociceptive neurons. (A, left) Normal CGRP staining in the DRG and superficial dorsal horn. Arrowhead indicates CGRP-expressing spinal motoneurons. (middle) CGRP expression is completely abolished in the DRG and its projections in TrkA/Bax double-null mice. CGRP staining in spinal motoneurons is not affected by loss of TrkA signaling (arrowhead). (right) CGRP expression in DRG is rescued by expression of kaB-RAF, in the absence of TrkA signaling ( LSL-kaBraf:nes-Cre:TrkA −/− :Bax −/− ). Arrowhead indicates the CGRP + motor neurons. Dashed white lines outline the spinal cord and DRG. (B) The nociceptive projection into the dorsal horn (left) does not depend on TrkA (middle). Expression of kaB-RAF causes overgrowth and ectopic targeting of these fibers (right). (A and B) Images are representative of three embryos each. (C) Activation of B-RAF does not directly induce CGRP expression in cultured DRG neurons. (top) No CGRP is expressed in 7-d in vitro cultures of dissociated E12.5 LSL-kaBraf:Bax −/− :nes-Cre DRG neurons. (bottom) NGF and conditioned medium from skin cultures are necessary to induce CGRP expression in E12.5 LSL-kaBraf:Bax −/− :nes-Cre DRG neurons. Images are representative of three independent experiments. This experiment has been repeated three times. Each experiment used two embryos per genotype. Bars: (A and B) 100 µm; (C) 20 µm.
Techniques Used: Activation Assay, Expressing, Staining, Mouse Assay, Cell Culture, In Vitro
5) Product Images from "Potentiation of Nerve Growth Factor-Induced Neurite Outgrowth in PC12 Cells by Ifenprodil: The Role of Sigma-1 and IP3 Receptors"
Article Title: Potentiation of Nerve Growth Factor-Induced Neurite Outgrowth in PC12 Cells by Ifenprodil: The Role of Sigma-1 and IP3 Receptors
Journal: PLoS ONE
Figure Legend Snippet: Effects of IP 3 receptor antagonists on NGF-induced neurite outgrowth in PC12 cells. (A): In the presence of NGF (2.5 ng/ml), vehicle, ifenprodil (10 µM), ifenprodil (10 µM)+xestospongin C (1.0 µM), xestospongin C (1.0 µM) were incubated with PC12 cells. (B): In the presence of NGF (2.5 ng/ml), vehicle, ifenprodil (10 µM), ifenprodil (10 µM)+2-APB (100 µM), or 2-APB (100 µM) were incubated in PC12 cells. Four days after incubation with test drugs, morphometric analysis was performed. The data show the mean ± SEM (n = 6). ***p
Techniques Used: Incubation
6) Product Images from "Phosphorylation of Serine 779 in Fibroblast Growth Factor Receptor 1 and 2 by Protein Kinase C? Regulates Ras/Mitogen-activated Protein Kinase Signaling and Neuronal Differentiation *"
Article Title: Phosphorylation of Serine 779 in Fibroblast Growth Factor Receptor 1 and 2 by Protein Kinase C? Regulates Ras/Mitogen-activated Protein Kinase Signaling and Neuronal Differentiation *
Journal: The Journal of Biological Chemistry
Figure Legend Snippet: Ser 779 in the cytoplasmic tail of FGFR1 and FGFR2 is required for maximal ERK activation. A–D , PC12 cells expressing PFR1 or PFR1-S779G ( A ) or wt-FGFR2 or FGFR2-S779G ( B–D ) were stimulated with PDGF-BB ( A ), FGF9 ( B ), EGF ( C ), or NGF (
Techniques Used: Activation Assay, Expressing
7) Product Images from "Analysis of Ret knockin mice reveals a critical role for IKKs, but not PI 3-K, in neurotrophic factor-induced survival of sympathetic neurons"
Article Title: Analysis of Ret knockin mice reveals a critical role for IKKs, but not PI 3-K, in neurotrophic factor-induced survival of sympathetic neurons
Journal: Cell death and differentiation
Figure Legend Snippet: IKKs are necessary for NGF- and GDNF-mediated survival of sympathetic neurons. (A and B) Neurons were infected with shRNAs to IKKs in combination (“α+ β”) or alone (“α” or “β”)
Techniques Used: Infection
Figure Legend Snippet: Neither PI 3-K/Akt nor ERK1/2 are necessary for GDNF-mediated survival. (A) Sympathetic neurons from wild type mice were cultured for five days in NGF and then switched to GDNF with or without the indicated doses of the PI 3-K inhibitor LY294002. Neuronal
Techniques Used: Mouse Assay, Cell Culture
Figure Legend Snippet: Knockdown of B-Raf, but not A-Raf or C-Raf, prevents GDNF-and NGF-mediated survival of wild type mouse sympathetic neurons. (A,B,C) Left panels, neurons were infected with lentiviruses expressing shRNAs to A-Raf (A1 and A2), B-Raf (B1 and B2) and C-Raf
Techniques Used: Infection, Expressing