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Cell Signaling Technology Inc nfatc1 nfat2 antibody
Nfatc1 Nfat2 Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/nfatc1 nfat2 antibody/product/Cell Signaling Technology Inc
Average 91 stars, based on 1 article reviews
Price from $9.99 to $1999.99
nfatc1 nfat2 antibody - by Bioz Stars, 2020-09
91/100 stars

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Incubation:

Article Title: Phospholipase C-related, but catalytically inactive protein (PRIP) up-regulates osteoclast differentiation via calcium-calcineurin-NFATc1 signaling
Article Snippet: .. The cells were then fixed in 4% paraformaldehyde in PBS for 15 min and permeabilized with 0.5% Triton X-100 for 30 min. After blocking with 2.5% BSA in PBS for 1 h, slide glasses were incubated with the NFATc1 (NFAT2) antibody (1:200; Cell Signaling Technology) overnight at 4 °C, washed with PBS, and then incubated with Alexa Fluor 488 donkey anti-rabbit IgG (1:1000; Molecular Probes, Eugene, OR) for 1 h at room temperature. .. Nuclei were counterstained with Hoechst 33258 (Sigma), and images were obtained using a BZ-9000 microscope (KEYENCE).

Blocking Assay:

Article Title: Phospholipase C-related, but catalytically inactive protein (PRIP) up-regulates osteoclast differentiation via calcium-calcineurin-NFATc1 signaling
Article Snippet: .. The cells were then fixed in 4% paraformaldehyde in PBS for 15 min and permeabilized with 0.5% Triton X-100 for 30 min. After blocking with 2.5% BSA in PBS for 1 h, slide glasses were incubated with the NFATc1 (NFAT2) antibody (1:200; Cell Signaling Technology) overnight at 4 °C, washed with PBS, and then incubated with Alexa Fluor 488 donkey anti-rabbit IgG (1:1000; Molecular Probes, Eugene, OR) for 1 h at room temperature. .. Nuclei were counterstained with Hoechst 33258 (Sigma), and images were obtained using a BZ-9000 microscope (KEYENCE).

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  • 92
    Cell Signaling Technology Inc nfatc1
    GW4064 induces NFAT translocation and calcineurin activation by a PI-PLC–dependent mechanism. A, GW4064 (GW) induction of intracellular Ca 2+ ) in a FACSCalibur flow cytometer. Data are representative of 3 independent experiments showing identical patterns. FITC, fluorescein isothiocyanate; V, vehicle. B, HEK cells in 10-cm dishes were treated with 1 μM GW4064, ionomycin (1 μM), or FK506 (10 μM) for 30 minutes and then were assayed for calcineurin activity using a colorimetric calcineurin assay kit. Recombinant human calcineurin (CaN, 40 U) was used as a positive control. Data represent nanomoles of PO 4 released (values are normalized to control) and are means ± SEM from 3 representative experiments performed in triplicate. C, HEK cells in chamber slides were treated with 1 μM GW4064 or ionomycin (I, 1 μM) for 30 minutes, and endogenous <t>NFATc1</t> was detected by immunocytochemical analysis. The nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI). Magnification, ×40; bars, 50 μm. Data are representative of 3 independent experiments. D, HEK cells in 24-well plates were transfected with 200 ng of NFAT-RE-Luc, and 100 ng of EGFPC1. Cells were then treated with the indicated compounds for 30 minutes followed by treatment with 1 μM GW4064 for 24 hours. Normalized luciferase values were then plotted as percent inhibition of GW4064 response. Data are means ± SEM of 3 independent experiments performed in duplicate. *, P
    Nfatc1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 92/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nfatc1/product/Cell Signaling Technology Inc
    Average 92 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    nfatc1 - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

    98
    Cell Signaling Technology Inc nfat2 d15f1 rabbit mab
    GW4064 induces NFAT translocation and calcineurin activation by a PI-PLC–dependent mechanism. A, GW4064 (GW) induction of intracellular Ca 2+ ) in a FACSCalibur flow cytometer. Data are representative of 3 independent experiments showing identical patterns. FITC, fluorescein isothiocyanate; V, vehicle. B, HEK cells in 10-cm dishes were treated with 1 μM GW4064, ionomycin (1 μM), or FK506 (10 μM) for 30 minutes and then were assayed for calcineurin activity using a colorimetric calcineurin assay kit. Recombinant human calcineurin (CaN, 40 U) was used as a positive control. Data represent nanomoles of PO 4 released (values are normalized to control) and are means ± SEM from 3 representative experiments performed in triplicate. C, HEK cells in chamber slides were treated with 1 μM GW4064 or ionomycin (I, 1 μM) for 30 minutes, and endogenous <t>NFATc1</t> was detected by immunocytochemical analysis. The nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI). Magnification, ×40; bars, 50 μm. Data are representative of 3 independent experiments. D, HEK cells in 24-well plates were transfected with 200 ng of NFAT-RE-Luc, and 100 ng of EGFPC1. Cells were then treated with the indicated compounds for 30 minutes followed by treatment with 1 μM GW4064 for 24 hours. Normalized luciferase values were then plotted as percent inhibition of GW4064 response. Data are means ± SEM of 3 independent experiments performed in duplicate. *, P
    Nfat2 D15f1 Rabbit Mab, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nfat2 d15f1 rabbit mab/product/Cell Signaling Technology Inc
    Average 98 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    nfat2 d15f1 rabbit mab - by Bioz Stars, 2020-09
    98/100 stars
      Buy from Supplier

    91
    Cell Signaling Technology Inc nfatc1 nfat2 antibody
    GW4064 induces NFAT translocation and calcineurin activation by a PI-PLC–dependent mechanism. A, GW4064 (GW) induction of intracellular Ca 2+ ) in a FACSCalibur flow cytometer. Data are representative of 3 independent experiments showing identical patterns. FITC, fluorescein isothiocyanate; V, vehicle. B, HEK cells in 10-cm dishes were treated with 1 μM GW4064, ionomycin (1 μM), or FK506 (10 μM) for 30 minutes and then were assayed for calcineurin activity using a colorimetric calcineurin assay kit. Recombinant human calcineurin (CaN, 40 U) was used as a positive control. Data represent nanomoles of PO 4 released (values are normalized to control) and are means ± SEM from 3 representative experiments performed in triplicate. C, HEK cells in chamber slides were treated with 1 μM GW4064 or ionomycin (I, 1 μM) for 30 minutes, and endogenous <t>NFATc1</t> was detected by immunocytochemical analysis. The nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI). Magnification, ×40; bars, 50 μm. Data are representative of 3 independent experiments. D, HEK cells in 24-well plates were transfected with 200 ng of NFAT-RE-Luc, and 100 ng of EGFPC1. Cells were then treated with the indicated compounds for 30 minutes followed by treatment with 1 μM GW4064 for 24 hours. Normalized luciferase values were then plotted as percent inhibition of GW4064 response. Data are means ± SEM of 3 independent experiments performed in duplicate. *, P
    Nfatc1 Nfat2 Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nfatc1 nfat2 antibody/product/Cell Signaling Technology Inc
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    nfatc1 nfat2 antibody - by Bioz Stars, 2020-09
    91/100 stars
      Buy from Supplier

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    GW4064 induces NFAT translocation and calcineurin activation by a PI-PLC–dependent mechanism. A, GW4064 (GW) induction of intracellular Ca 2+ ) in a FACSCalibur flow cytometer. Data are representative of 3 independent experiments showing identical patterns. FITC, fluorescein isothiocyanate; V, vehicle. B, HEK cells in 10-cm dishes were treated with 1 μM GW4064, ionomycin (1 μM), or FK506 (10 μM) for 30 minutes and then were assayed for calcineurin activity using a colorimetric calcineurin assay kit. Recombinant human calcineurin (CaN, 40 U) was used as a positive control. Data represent nanomoles of PO 4 released (values are normalized to control) and are means ± SEM from 3 representative experiments performed in triplicate. C, HEK cells in chamber slides were treated with 1 μM GW4064 or ionomycin (I, 1 μM) for 30 minutes, and endogenous NFATc1 was detected by immunocytochemical analysis. The nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI). Magnification, ×40; bars, 50 μm. Data are representative of 3 independent experiments. D, HEK cells in 24-well plates were transfected with 200 ng of NFAT-RE-Luc, and 100 ng of EGFPC1. Cells were then treated with the indicated compounds for 30 minutes followed by treatment with 1 μM GW4064 for 24 hours. Normalized luciferase values were then plotted as percent inhibition of GW4064 response. Data are means ± SEM of 3 independent experiments performed in duplicate. *, P

    Journal: Molecular Endocrinology

    Article Title: Synthetic FXR Agonist GW4064 Is a Modulator of Multiple G Protein–Coupled Receptors

    doi: 10.1210/me.2013-1353

    Figure Lengend Snippet: GW4064 induces NFAT translocation and calcineurin activation by a PI-PLC–dependent mechanism. A, GW4064 (GW) induction of intracellular Ca 2+ ) in a FACSCalibur flow cytometer. Data are representative of 3 independent experiments showing identical patterns. FITC, fluorescein isothiocyanate; V, vehicle. B, HEK cells in 10-cm dishes were treated with 1 μM GW4064, ionomycin (1 μM), or FK506 (10 μM) for 30 minutes and then were assayed for calcineurin activity using a colorimetric calcineurin assay kit. Recombinant human calcineurin (CaN, 40 U) was used as a positive control. Data represent nanomoles of PO 4 released (values are normalized to control) and are means ± SEM from 3 representative experiments performed in triplicate. C, HEK cells in chamber slides were treated with 1 μM GW4064 or ionomycin (I, 1 μM) for 30 minutes, and endogenous NFATc1 was detected by immunocytochemical analysis. The nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI). Magnification, ×40; bars, 50 μm. Data are representative of 3 independent experiments. D, HEK cells in 24-well plates were transfected with 200 ng of NFAT-RE-Luc, and 100 ng of EGFPC1. Cells were then treated with the indicated compounds for 30 minutes followed by treatment with 1 μM GW4064 for 24 hours. Normalized luciferase values were then plotted as percent inhibition of GW4064 response. Data are means ± SEM of 3 independent experiments performed in duplicate. *, P

    Article Snippet: Rabbit FXR (sc-13063) and mouse β-actin (sc-47778) antibodies were from Santa Cruz Biotechnology Inc. NFATc1 (NFAT2), transducer of regulated CRE-binding protein (TORC) 2, phospho-CRE-binding protein (CREB) (S133), and ERK antibodies were from Cell Signaling Technology.

    Techniques: Translocation Assay, Activation Assay, Planar Chromatography, Flow Cytometry, Cytometry, Activity Assay, Recombinant, Positive Control, Staining, Transfection, Luciferase, Inhibition