nextseq 500 sequencing platform  (Illumina Inc)

 
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Name:
    NextSeq 500 Installation and Operational Qualification
    Description:
    Comprehensive Qualification Services from Illumina Illumina Installation and Operational Qualification IQ OQ services carry out numerous experiments for each system to ensure critical components are tested and validated have cut off metrics and to confirm these metrics show the system is operating in accordance with specifications Our IQ service provides documented verification that instrument is installed according to our specifications and safety regulations During the IQ a trained engineer verifies latest supported firmware and software versions were installed verifies instrument setup and accessory logistics checks physical and environmental safety conditions are met and provides a signed audit ready report Our OQ service follows a comprehensive well defined protocol to verify the system functions according to pre set and validated operational specifications We update the OQ protocol following each instrument hardware and software release ensuring your laboratory receives the most up to date service Critical aspects of the OQ include motion qualification optics qualification fluidics qualification and thermal qualification As regulatory guidelines continue to evolve and increase in stringency laboratories need well documented system qualification protocols for compliance Performed by Illumina trained and certified engineers IQ OQ helps research and diagnostic genomic laboratories meet regulatory guidelines Provides an audit ready signed IQ OQ report Accelerates use by ensuring the Illumina instrument is installed operational and ready to use after initial delivery Verifies Illumina sequencers perform according to manufacturer specifications after major repairs relocation or system software upgrades Helps establish instrument log files for change control Provides engineer training and tool calibration certificates upon customer request
    Catalog Number:
    sp-104-1006
    Price:
    None
    Category:
    Service Products
    Buy from Supplier


    Structured Review

    Illumina Inc nextseq 500 sequencing platform
    NextSeq 500 Installation and Operational Qualification
    Comprehensive Qualification Services from Illumina Illumina Installation and Operational Qualification IQ OQ services carry out numerous experiments for each system to ensure critical components are tested and validated have cut off metrics and to confirm these metrics show the system is operating in accordance with specifications Our IQ service provides documented verification that instrument is installed according to our specifications and safety regulations During the IQ a trained engineer verifies latest supported firmware and software versions were installed verifies instrument setup and accessory logistics checks physical and environmental safety conditions are met and provides a signed audit ready report Our OQ service follows a comprehensive well defined protocol to verify the system functions according to pre set and validated operational specifications We update the OQ protocol following each instrument hardware and software release ensuring your laboratory receives the most up to date service Critical aspects of the OQ include motion qualification optics qualification fluidics qualification and thermal qualification As regulatory guidelines continue to evolve and increase in stringency laboratories need well documented system qualification protocols for compliance Performed by Illumina trained and certified engineers IQ OQ helps research and diagnostic genomic laboratories meet regulatory guidelines Provides an audit ready signed IQ OQ report Accelerates use by ensuring the Illumina instrument is installed operational and ready to use after initial delivery Verifies Illumina sequencers perform according to manufacturer specifications after major repairs relocation or system software upgrades Helps establish instrument log files for change control Provides engineer training and tool calibration certificates upon customer request
    https://www.bioz.com/result/nextseq 500 sequencing platform/product/Illumina Inc
    Average 99 stars, based on 66 article reviews
    Price from $9.99 to $1999.99
    nextseq 500 sequencing platform - by Bioz Stars, 2020-07
    99/100 stars

    Images

    Related Articles

    Flow Cytometry:

    Article Title: Blue Light Sensing in Listeria monocytogenes Is Temperature-Dependent and the Transcriptional Response to It Is Predominantly SigB-Dependent
    Article Snippet: .. Indexed and purified libraries were loaded together onto a flow cell, and sequencing was carried out on the Illumina NextSeq 500 platform (paired-end, 2 × 75 bp per read). .. Sequencing quality was assessed using FastQC and Illumina adapter sequences, and low-quality base pairs were removed using CutAdapt version 1.9 ( ).

    RNA Sequencing Assay:

    Article Title: Receptor-specific interactome as a hub for rapid cue-induced selective translation in axons
    Article Snippet: .. The cDNA library preparation was performed using a KAPA Hyperprep kit (Roche) and cDNA libraries were subjected to a RNA-sequencing run on a Next-seq 500 instrument (Illumina) using the 150 cycles high output kit (Illumina). .. Bioinformatic analysis of RNA-sequencing data The sequence reads were mapped using HISAT 2 version 2.1.0, and FPKM values were estimated using Cufflinks version 2.2.1.

    Article Title: Genome-wide profiling of the PIWI-interacting RNA-mRNA regulatory networks in epithelial ovarian cancers
    Article Snippet: .. Genotypic Technology, Bangalore, India carried out small RNA sequencing on Illumina Next-Seq 500 platform. ..

    Construct:

    Article Title: Machine Learning Enables High-Throughput Phenotyping for Analyses of the Genetic Architecture of Bulliform Cell Patterning in Maize
    Article Snippet: .. GBS libraries were constructed and multiplexed 192-fold for sequencing on an Illumina NextSeq 500 instrument. ..

    Purification:

    Article Title: Blue Light Sensing in Listeria monocytogenes Is Temperature-Dependent and the Transcriptional Response to It Is Predominantly SigB-Dependent
    Article Snippet: .. Indexed and purified libraries were loaded together onto a flow cell, and sequencing was carried out on the Illumina NextSeq 500 platform (paired-end, 2 × 75 bp per read). .. Sequencing quality was assessed using FastQC and Illumina adapter sequences, and low-quality base pairs were removed using CutAdapt version 1.9 ( ).

    cDNA Library Assay:

    Article Title: Receptor-specific interactome as a hub for rapid cue-induced selective translation in axons
    Article Snippet: .. The cDNA library preparation was performed using a KAPA Hyperprep kit (Roche) and cDNA libraries were subjected to a RNA-sequencing run on a Next-seq 500 instrument (Illumina) using the 150 cycles high output kit (Illumina). .. Bioinformatic analysis of RNA-sequencing data The sequence reads were mapped using HISAT 2 version 2.1.0, and FPKM values were estimated using Cufflinks version 2.2.1.

    Sequencing:

    Article Title: Novel gRNA design pipeline to develop broad-spectrum CRISPR/Cas9 gRNAs for safe targeting of the HIV-1 quasispecies in patients
    Article Snippet: .. Sequencing was performed on an Illumina NextSeq 500 instrument in paired-ended mode with a 300 High Output v2 sequencing procedure, as described by the manufacturer. .. This produced approximately 10 million reads per sample, providing the ability to query the diversity of the HIV-1 LTR vQS within each patient’s PBMCs.

    Article Title: Machine Learning Enables High-Throughput Phenotyping for Analyses of the Genetic Architecture of Bulliform Cell Patterning in Maize
    Article Snippet: .. GBS libraries were constructed and multiplexed 192-fold for sequencing on an Illumina NextSeq 500 instrument. ..

    Article Title: Blue Light Sensing in Listeria monocytogenes Is Temperature-Dependent and the Transcriptional Response to It Is Predominantly SigB-Dependent
    Article Snippet: .. Indexed and purified libraries were loaded together onto a flow cell, and sequencing was carried out on the Illumina NextSeq 500 platform (paired-end, 2 × 75 bp per read). .. Sequencing quality was assessed using FastQC and Illumina adapter sequences, and low-quality base pairs were removed using CutAdapt version 1.9 ( ).

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Illumina Inc illumina nextseq platform
    Experimental layout for in vitro infection of the intestine. Confluent rat intestinal epithelial cells (IEC-18) were infected with either sporozoites (SPZ) or tachyzoites in the absence (TZ) or presence (TZ+fzSPZ) of frozen-thawed sporozoites for 8 hours at 37°C. As a control for possible effects of oocyst/sporocyst wall components and MA-104 cells debris, sporozoites inactivated by freezing (fzSPZ) or MA-104 cell lysates (Mock) were added to IEC-18 cells, respectively. All experiments were performed in biological duplicate (i.e., starting with individual populations of sporozoites) and with two technical replicates. Total RNA was extracted and RNA sequencing was performed using the <t>Illumina</t> <t>NextSeq</t> platform. SAMseq analysis was used to identify differentially regulated genes of both host and parasite origin in various pairwise comparisons.
    Illumina Nextseq Platform, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 803 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/illumina nextseq platform/product/Illumina Inc
    Average 99 stars, based on 803 article reviews
    Price from $9.99 to $1999.99
    illumina nextseq platform - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    Image Search Results


    Experimental layout for in vitro infection of the intestine. Confluent rat intestinal epithelial cells (IEC-18) were infected with either sporozoites (SPZ) or tachyzoites in the absence (TZ) or presence (TZ+fzSPZ) of frozen-thawed sporozoites for 8 hours at 37°C. As a control for possible effects of oocyst/sporocyst wall components and MA-104 cells debris, sporozoites inactivated by freezing (fzSPZ) or MA-104 cell lysates (Mock) were added to IEC-18 cells, respectively. All experiments were performed in biological duplicate (i.e., starting with individual populations of sporozoites) and with two technical replicates. Total RNA was extracted and RNA sequencing was performed using the Illumina NextSeq platform. SAMseq analysis was used to identify differentially regulated genes of both host and parasite origin in various pairwise comparisons.

    Journal: PLoS ONE

    Article Title: An in vitro model of intestinal infection reveals a developmentally regulated transcriptome of Toxoplasma sporozoites and a NF-κB-like signature in infected host cells

    doi: 10.1371/journal.pone.0173018

    Figure Lengend Snippet: Experimental layout for in vitro infection of the intestine. Confluent rat intestinal epithelial cells (IEC-18) were infected with either sporozoites (SPZ) or tachyzoites in the absence (TZ) or presence (TZ+fzSPZ) of frozen-thawed sporozoites for 8 hours at 37°C. As a control for possible effects of oocyst/sporocyst wall components and MA-104 cells debris, sporozoites inactivated by freezing (fzSPZ) or MA-104 cell lysates (Mock) were added to IEC-18 cells, respectively. All experiments were performed in biological duplicate (i.e., starting with individual populations of sporozoites) and with two technical replicates. Total RNA was extracted and RNA sequencing was performed using the Illumina NextSeq platform. SAMseq analysis was used to identify differentially regulated genes of both host and parasite origin in various pairwise comparisons.

    Article Snippet: Multiplex sequencing libraries were generated with RNA Sample Prep Kit (Illumina) according to manufacturer’s instructions and pooled for a single high-throughput sequencing run using the Illumina NextSeq platform (Illumina Nextseq 500 model instrument).

    Techniques: In Vitro, Infection, RNA Sequencing Assay

    Schematic circular representation of complete genome sequences of KPC‐2‐producing A. hydrophila GSH8‐2. Short paired‐end whole‐genome sequencing was performed using an Illumina NextSeq 500 platform with a 300‐cycle NextSeq 500 Reagent Kit v2 (2 × 150‐mer). The complete genome sequences of the strains were determined using a PacBio Sequel sequencer for long‐read sequencing [Sequel SMRT Cell 1 M v2 (4/tray]; Sequel sequencing kit v2.1; insert size, approximately 10 kb). De novo assembly was performed using Canu version 1.4 (Koren et al ., 2017 ), minimap version 0.2‐r124 (Li, 2016 ), racon version 1.1.0 (Vaser et al ., 2017 ) and circulator version 1.5.3 (Hunt et al ., 2015 ). Error correction of tentative complete circular sequences was performed using Pilon version 1.18 with Illumina short reads (Walker et al ., 2014 ). Annotation was performed in Prokka version 1.11 (Seemann, 2014 ), InterPro v49.0 (Finn et al ., 2017 ) and NCBI‐BLASTP/BLASTX. Circular representations of complete genomic sequences were visualized using GView server (Petkau et al ., 2010 ). AMR genes were identified by homology searching against the ResFinder database (Zankari et al ., 2012 ). The class 1 integron was assigned in the INTEGRALL database ( http://integrall.bio.ua.pt/ ) (Moura et al ., 2009 ). Visualization of comparative plasmid ORFs organization was performed using Easyfig (Sullivan et al ., 2011 ). For representation of chromosomal DNA, from the inside: slot 1, GC skew; slot 2, GC content; slot 3, ORFs; slot 4, rRNA/tRNA; slots 5–7, BLASTatlas conserved gene analysis indicating three relative strains (see also Supporting Information Fig. S1 ); slot 8, prophage; slot 9, notable ARGs or ARG‐related genes (transposase, ARGs and reductases). In representations of circular plasmids, notable ORFs are highlighted as the indicated colour.

    Journal: Environmental Microbiology Reports

    Article Title: Potential KPC‐2 carbapenemase reservoir of environmental Aeromonas hydrophila and Aeromonas caviae isolates from the effluent of an urban wastewater treatment plant in Japan

    doi: 10.1111/1758-2229.12772

    Figure Lengend Snippet: Schematic circular representation of complete genome sequences of KPC‐2‐producing A. hydrophila GSH8‐2. Short paired‐end whole‐genome sequencing was performed using an Illumina NextSeq 500 platform with a 300‐cycle NextSeq 500 Reagent Kit v2 (2 × 150‐mer). The complete genome sequences of the strains were determined using a PacBio Sequel sequencer for long‐read sequencing [Sequel SMRT Cell 1 M v2 (4/tray]; Sequel sequencing kit v2.1; insert size, approximately 10 kb). De novo assembly was performed using Canu version 1.4 (Koren et al ., 2017 ), minimap version 0.2‐r124 (Li, 2016 ), racon version 1.1.0 (Vaser et al ., 2017 ) and circulator version 1.5.3 (Hunt et al ., 2015 ). Error correction of tentative complete circular sequences was performed using Pilon version 1.18 with Illumina short reads (Walker et al ., 2014 ). Annotation was performed in Prokka version 1.11 (Seemann, 2014 ), InterPro v49.0 (Finn et al ., 2017 ) and NCBI‐BLASTP/BLASTX. Circular representations of complete genomic sequences were visualized using GView server (Petkau et al ., 2010 ). AMR genes were identified by homology searching against the ResFinder database (Zankari et al ., 2012 ). The class 1 integron was assigned in the INTEGRALL database ( http://integrall.bio.ua.pt/ ) (Moura et al ., 2009 ). Visualization of comparative plasmid ORFs organization was performed using Easyfig (Sullivan et al ., 2011 ). For representation of chromosomal DNA, from the inside: slot 1, GC skew; slot 2, GC content; slot 3, ORFs; slot 4, rRNA/tRNA; slots 5–7, BLASTatlas conserved gene analysis indicating three relative strains (see also Supporting Information Fig. S1 ); slot 8, prophage; slot 9, notable ARGs or ARG‐related genes (transposase, ARGs and reductases). In representations of circular plasmids, notable ORFs are highlighted as the indicated colour.

    Article Snippet: These isolates were then subjected to whole‐genome sequencing (WGS) as described previously (Sekizuka et al ., ), using an Illumina NextSeq 500 platform with a 300‐cycle NextSeq 500 Reagent Kit v2 (2 × 150‐mer).

    Techniques: Sequencing, Genomic Sequencing, Plasmid Preparation