Structured Review

Illumina Inc nextseq 500 sequencing platform
Nextseq 500 Sequencing Platform, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 97/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/nextseq 500 sequencing platform/product/Illumina Inc
Average 97 stars, based on 9 article reviews
Price from $9.99 to $1999.99
nextseq 500 sequencing platform - by Bioz Stars, 2019-12
97/100 stars

Images

Related Articles

Centrifugation:

Article Title: Structural and functional adaptation of Haloferax volcanii TFEα/β
Article Snippet: RNA was pelleted by centrifugation at 20 000 x g for 10 min, washed with 75% ethanol, air-dried and resuspended in 50 μl of RNase-free dH2 O. .. Sequencing was performed on the Illumina NextSeq 500 sequencing platform to generate 2 × 75 bp paired end reads using V2 chemistry.

Amplification:

Article Title: Circulating Exosomal miRNA Profile During Term and Preterm Birth Pregnancies: A Longitudinal Study
Article Snippet: The elution containing the pooled DNA library was further processed for cluster generation and sequencing using a NextSeq 500 high-output kit 75 cycles and Illumina NextSeq 500 sequencing platform, respectively. .. The elution containing the pooled DNA library was further processed for cluster generation and sequencing using a NextSeq 500 high-output kit 75 cycles and Illumina NextSeq 500 sequencing platform, respectively.

Article Title: Inhibition of methyltransferase Setd7 allows the in vitro expansion of myogenic stem cells with improved therapeutic potential
Article Snippet: Illumina sequencing libraries were generated by end repair, 3’ A-addition, and Illumina sequencing adaptor ligation (New England BioLabs, E6000B-10). .. Libraries were then indexed and PCR amplified (10 cycles) and sequenced using a NextSeq 500 High-Output Kit (Illumina) on a Illumina NextSeq 500 sequencing platform following the manufacturer’s protocols (Illumina). .. Sequence reads were mapped to mm10 (GRCm38) using Burrows-Wheeler Aligner (BWA) ( ).

Article Title: Oxygen tension regulates the miRNA profile and bioactivity of exosomes released from extravillous trophoblast cells – Liquid biopsies for monitoring complications of pregnancy
Article Snippet: The elution containing the pooled DNA library was further processed for cluster generation and sequencing using NextSeq 500 High Output kit 75 cycles and Illumina NextSeq 500 sequencing platform, respectively. .. The elution containing the pooled DNA library was further processed for cluster generation and sequencing using NextSeq 500 High Output kit 75 cycles and Illumina NextSeq 500 sequencing platform, respectively.

Article Title: Versatile approach for functional analysis of human proteins and efficient stable cell line generation using FLP-mediated recombination system
Article Snippet: TruSeq kit protocol temperature scheme with 12 amplification cycles and subsequent purification procedure was applied. .. Sequencing was carried out on an Illumina NextSeq 500 sequencing platform, using NextSeq 500 High Output Kit (150 cycles) (FC-404-1002, Illumina) and standard libraries denaturation and pair-end sequencing procedures (Instructions: 15048776 Rev.

Synthesized:

Article Title: Daily changes in phytoplankton lipidomes reveal mechanisms of energy storage in the open ocean
Article Snippet: In addition, to generate quantitative transcript inventories, a set of 14 internal mRNA standards were also added to the extraction buffer; these standards were synthesized according to Satinski et al. with the exception that eight standards were synthesized with poly(A) tails to mimic eukaryotic mRNAs. .. Briefly, RNAs were poly(A)-selected, converted to cDNAs, and sheared to ~225 bp fragments which were used to prepare cDNA libraries for paired-end (2 × 150) sequencing using the Illumina NextSeq 500 sequencing platform (Illumina Inc., San Diego, CA).

Cell Isolation:

Article Title: Differential effects of FTY720 on the B cell compartment in a mouse model of multiple sclerosis
Article Snippet: B cells were purified from the spleens of MP4-immunized mice using a commercially available Mouse B Cell Isolation Kit (Miltenyi Biotec, Bergisch Gladbach, Germany). .. Subsequently, all libraries were sequenced with a high output 1 × 75 cycles kit using the Illumina Nextseq 500 sequencing platform that generated 75 bp long single-end reads.

Construct:

Article Title: Daily changes in phytoplankton lipidomes reveal mechanisms of energy storage in the open ocean
Article Snippet: Purified total RNA was used to construct TruSeq cDNA libraries according to the Illumina TruSeq® RNA Sample Preparation v2 Guide. .. Briefly, RNAs were poly(A)-selected, converted to cDNAs, and sheared to ~225 bp fragments which were used to prepare cDNA libraries for paired-end (2 × 150) sequencing using the Illumina NextSeq 500 sequencing platform (Illumina Inc., San Diego, CA).

Article Title: Metastatic ability and the epithelial‐mesenchymal transition in induced cancer stem‐like hepatoma cells, et al. Metastatic ability and the epithelial‐mesenchymal transition in induced cancer stem‐like hepatoma cells
Article Snippet: Sequencing libraries were constructed using the TruSeq Stranded Total RNA with Ribo‐Zero Gold LT Sample Prep kit (Illumina, Tokyo, Japan) according to manufacturer's instructions. .. Sequencing of paired‐end fragments (75 bp × 2) was carried out on a NextSeq 500 sequencing platform (Illumina) to a depth, in triplicate, of 123‐150 million fragments for SK‐HEP‐1 and SK‐sphere cells, and a depth, in duplicate, of 42‐46 million fragments for HuH‐7 cells.

Real-time Polymerase Chain Reaction:

Article Title: Versatile approach for functional analysis of human proteins and efficient stable cell line generation using FLP-mediated recombination system
Article Snippet: The libraries’ concentration was estimated by qPCR means with KAPA Universal Library Quantification Kit (KK4824, Kapa Biosystems), according to the supplied protocol. .. Sequencing was carried out on an Illumina NextSeq 500 sequencing platform, using NextSeq 500 High Output Kit (150 cycles) (FC-404-1002, Illumina) and standard libraries denaturation and pair-end sequencing procedures (Instructions: 15048776 Rev.

Incubation:

Article Title: Inhibition of methyltransferase Setd7 allows the in vitro expansion of myogenic stem cells with improved therapeutic potential
Article Snippet: This antibody-bead mix was then incubated with the digested chromatin overnight at 4° in IP buffer (20mM Tris-HCl pH 7.5, 2mM EDTA, 150mM NaCl, 0.1% Triton X-100, 0.1% Deoxycholate). .. Libraries were then indexed and PCR amplified (10 cycles) and sequenced using a NextSeq 500 High-Output Kit (Illumina) on a Illumina NextSeq 500 sequencing platform following the manufacturer’s protocols (Illumina).

Expressing:

Article Title: Differential effects of FTY720 on the B cell compartment in a mouse model of multiple sclerosis
Article Snippet: Paragraph title: RNA-seq expression profiling ... Subsequently, all libraries were sequenced with a high output 1 × 75 cycles kit using the Illumina Nextseq 500 sequencing platform that generated 75 bp long single-end reads.

Article Title: RNA sequencing analysis of the developing chicken retina
Article Snippet: Total RNA samples chosen for characterization of global mRNA expression were submitted to the Cold Spring Harbor Laboratory DNA Sequencing Center for Bioanalyzer quality control analysis (Agilent) and Illumina Next Generation Sequencing. .. Libraries for the 8 cDNA samples were sequenced using the Illumina NextSeq 500 sequencing platform yielding 28.6–72.2 million 125 bp paired end sequence reads per sample ( ; ).

Flow Cytometry:

Article Title: Alternative Growth Promoters Modulate Broiler Gut Microbiome and Enhance Body Weight Gain
Article Snippet: Nextera DNA libraries were made for each of the 20 samples separately using Nextera DNA Library Preparation Kit and Nextera Index Kit (Illumina, San Diego, CA, United States) followed by pooling into equimolar concentrations according to the manufacturer’s instructions. .. Paired-end sequencing (2 × 151 bp) was conducted on an Illumina NextSeq 500 sequencing platform with a NextSeq 500/550 v2 High Output flow cell. .. Metagenomic sequence data have been deposited at NCBI under accession numbers SRR5280289 , SRR5280393 , SRR5280514 , SRR5280757 , SRR5280758 , SRR5280759 , SRR5281813 , SRR5281814 , SRR5281815 , SRR5282096 , SRR5282097 , and SRR5282098 .

Article Title: Dedicated surveillance mechanism controls G-quadruplex forming non-coding RNAs in human mitochondria
Article Snippet: B, 15035788 Rev. .. Sequencing (Figs and ) was carried out on an Illumina NextSeq 500 sequencing platform, using NextSeq 500 High Output Kit (150 cycles) (FC-404-1002, Illumina) and standard libraries denaturation and pair-end sequencing procedures of 2 × 75 cycles (Instructions: 15048776 Rev. .. D, 15046563 Rev.

Article Title: Fecal Metagenome Sequences from Lactating Dairy Cows Shedding Escherichia coli O157:H7
Article Snippet: Nextera DNA library preparation kits (Illumina, San Diego, CA) were used to prepare sequencing libraries of the 10 samples. .. Paired-end sequencing (2 × 151 bp) was conducted on an Illumina NextSeq 500 sequencing platform with a NextSeq 500/550 v2 high-output flow cell. .. Raw reads were demultiplexed using the BCL2FastQ program and cleaned using DeconSeq ( ) and Trimmomatic v0.36 ( ).

Article Title: Versatile approach for functional analysis of human proteins and efficient stable cell line generation using FLP-mediated recombination system
Article Snippet: The libraries’ concentration was estimated by qPCR means with KAPA Universal Library Quantification Kit (KK4824, Kapa Biosystems), according to the supplied protocol. .. Sequencing was carried out on an Illumina NextSeq 500 sequencing platform, using NextSeq 500 High Output Kit (150 cycles) (FC-404-1002, Illumina) and standard libraries denaturation and pair-end sequencing procedures (Instructions: 15048776 Rev. .. D, 15046563 Rev.

Article Title: Genomic and Evolutionary Analysis of Two Salmonella enterica Serovar Kentucky Sequence Types Isolated from Bovine and Poultry Sources in North America
Article Snippet: Nextera XT libraries were made for each sample and pooled into equimolar concentrations following the manufacturer’s instructions (Illumina, San Diego, CA). .. Paired-end sequencing (2 X 151 bp) was conducted on an Illumina NextSeq 500 sequencing platform with a High-Output flow cell. .. Data were demultiplexed and trimmed to remove adaptor sequences using the BCL2FastQ program and PhiX reads were removed using DeconSeq [ ].

RAST Test:

Article Title: Evolution of tigecycline- and colistin-resistant CRKP (carbapenem-resistant Klebsiella pneumoniae) in vivo and its persistence in the GI tract
Article Snippet: Genomic sequencing was performed by means of the Illumina NextSeq 500 sequencing platform. .. Raw Illumina reads were trimmed or filtered to remove low-quality sequences and adapters, and assembled de novo by the SPAdes Genome Assembler v3.9.1 .

Ligation:

Article Title: Circulating Exosomal miRNA Profile During Term and Preterm Birth Pregnancies: A Longitudinal Study
Article Snippet: These RNA samples were barcoded by ligation with unique adaptor sequences to allow pooling of samples in groups of 24. .. The elution containing the pooled DNA library was further processed for cluster generation and sequencing using a NextSeq 500 high-output kit 75 cycles and Illumina NextSeq 500 sequencing platform, respectively.

Article Title: Inhibition of methyltransferase Setd7 allows the in vitro expansion of myogenic stem cells with improved therapeutic potential
Article Snippet: Illumina sequencing libraries were generated by end repair, 3’ A-addition, and Illumina sequencing adaptor ligation (New England BioLabs, E6000B-10). .. Libraries were then indexed and PCR amplified (10 cycles) and sequenced using a NextSeq 500 High-Output Kit (Illumina) on a Illumina NextSeq 500 sequencing platform following the manufacturer’s protocols (Illumina).

Article Title: Oxygen tension regulates the miRNA profile and bioactivity of exosomes released from extravillous trophoblast cells – Liquid biopsies for monitoring complications of pregnancy
Article Snippet: These RNA samples were barcoded by ligation with unique adaptor sequences to allow pooling of samples in groups of 24. .. The elution containing the pooled DNA library was further processed for cluster generation and sequencing using NextSeq 500 High Output kit 75 cycles and Illumina NextSeq 500 sequencing platform, respectively.

Article Title: Genome-wide mapping of sister chromatid exchange events in single yeast cells using Strand-seq
Article Snippet: After ligation and PCR, DNA clean-up with magnetic AMPure XP beads (Agencourt AMPure, Beckman Coulter) was performed twice using a 1.2 vol of beads. .. Single-end 50 bp sequencing reads from the Strand-seq libraries were generated using the HiSeq 2500 or the NextSeq 500 sequencing platform (Illumina, San Diego, CA).

Genomic Sequencing:

Article Title: Evolution of tigecycline- and colistin-resistant CRKP (carbapenem-resistant Klebsiella pneumoniae) in vivo and its persistence in the GI tract
Article Snippet: STs were assigned using online database tools. .. Genomic sequencing was performed by means of the Illumina NextSeq 500 sequencing platform. .. Raw Illumina reads were trimmed or filtered to remove low-quality sequences and adapters, and assembled de novo by the SPAdes Genome Assembler v3.9.1 .

Generated:

Article Title: Differential effects of FTY720 on the B cell compartment in a mouse model of multiple sclerosis
Article Snippet: B cells were purified from the spleens of MP4-immunized mice using a commercially available Mouse B Cell Isolation Kit (Miltenyi Biotec, Bergisch Gladbach, Germany). .. Subsequently, all libraries were sequenced with a high output 1 × 75 cycles kit using the Illumina Nextseq 500 sequencing platform that generated 75 bp long single-end reads. .. The generated raw reads were processed using FastQC 0.11.3 for assessing read quality, amount of duplicates, and presence of adapter sequences.

Article Title: Circulating Exosomal miRNA Profile During Term and Preterm Birth Pregnancies: A Longitudinal Study
Article Snippet: Sequencing libraries were generated using the TruSeq Small RNA Library Prep Kit (Illumina, San Diego, CA), according to the manufacturer’s instructions, and as previously described ( , ). .. The elution containing the pooled DNA library was further processed for cluster generation and sequencing using a NextSeq 500 high-output kit 75 cycles and Illumina NextSeq 500 sequencing platform, respectively.

Article Title: Inhibition of methyltransferase Setd7 allows the in vitro expansion of myogenic stem cells with improved therapeutic potential
Article Snippet: Illumina sequencing libraries were generated by end repair, 3’ A-addition, and Illumina sequencing adaptor ligation (New England BioLabs, E6000B-10). .. Libraries were then indexed and PCR amplified (10 cycles) and sequenced using a NextSeq 500 High-Output Kit (Illumina) on a Illumina NextSeq 500 sequencing platform following the manufacturer’s protocols (Illumina).

Article Title: Oxygen tension regulates the miRNA profile and bioactivity of exosomes released from extravillous trophoblast cells – Liquid biopsies for monitoring complications of pregnancy
Article Snippet: Sequencing libraries were generated using the TruSeq® SmallRNA Library Prep Kit, according to the manufacturer’s instructions. .. The elution containing the pooled DNA library was further processed for cluster generation and sequencing using NextSeq 500 High Output kit 75 cycles and Illumina NextSeq 500 sequencing platform, respectively.

Article Title: Genome-wide mapping of sister chromatid exchange events in single yeast cells using Strand-seq
Article Snippet: After ligation and PCR, DNA clean-up with magnetic AMPure XP beads (Agencourt AMPure, Beckman Coulter) was performed twice using a 1.2 vol of beads. .. Single-end 50 bp sequencing reads from the Strand-seq libraries were generated using the HiSeq 2500 or the NextSeq 500 sequencing platform (Illumina, San Diego, CA). .. Reads were aligned to the S. cerevisiae EF4 reference genome using Bowtie2 (version 2.0.0-beta7; ) and analyzed using the BAIT software package ( ) for data selection and visualization purposes.

DNA Sequencing:

Article Title: RNA sequencing analysis of the developing chicken retina
Article Snippet: Total RNA samples chosen for characterization of global mRNA expression were submitted to the Cold Spring Harbor Laboratory DNA Sequencing Center for Bioanalyzer quality control analysis (Agilent) and Illumina Next Generation Sequencing. .. Libraries for the 8 cDNA samples were sequenced using the Illumina NextSeq 500 sequencing platform yielding 28.6–72.2 million 125 bp paired end sequence reads per sample ( ; ).

Polymerase Chain Reaction:

Article Title: Circulating Exosomal miRNA Profile During Term and Preterm Birth Pregnancies: A Longitudinal Study
Article Snippet: The elution containing the pooled DNA library was further processed for cluster generation and sequencing using a NextSeq 500 high-output kit 75 cycles and Illumina NextSeq 500 sequencing platform, respectively. .. The elution containing the pooled DNA library was further processed for cluster generation and sequencing using a NextSeq 500 high-output kit 75 cycles and Illumina NextSeq 500 sequencing platform, respectively.

Article Title: Inhibition of methyltransferase Setd7 allows the in vitro expansion of myogenic stem cells with improved therapeutic potential
Article Snippet: Illumina sequencing libraries were generated by end repair, 3’ A-addition, and Illumina sequencing adaptor ligation (New England BioLabs, E6000B-10). .. Libraries were then indexed and PCR amplified (10 cycles) and sequenced using a NextSeq 500 High-Output Kit (Illumina) on a Illumina NextSeq 500 sequencing platform following the manufacturer’s protocols (Illumina). .. Sequence reads were mapped to mm10 (GRCm38) using Burrows-Wheeler Aligner (BWA) ( ).

Article Title: Oxygen tension regulates the miRNA profile and bioactivity of exosomes released from extravillous trophoblast cells – Liquid biopsies for monitoring complications of pregnancy
Article Snippet: The elution containing the pooled DNA library was further processed for cluster generation and sequencing using NextSeq 500 High Output kit 75 cycles and Illumina NextSeq 500 sequencing platform, respectively. .. The elution containing the pooled DNA library was further processed for cluster generation and sequencing using NextSeq 500 High Output kit 75 cycles and Illumina NextSeq 500 sequencing platform, respectively.

Article Title: Genome-wide mapping of sister chromatid exchange events in single yeast cells using Strand-seq
Article Snippet: After ligation and PCR, DNA clean-up with magnetic AMPure XP beads (Agencourt AMPure, Beckman Coulter) was performed twice using a 1.2 vol of beads. .. Single-end 50 bp sequencing reads from the Strand-seq libraries were generated using the HiSeq 2500 or the NextSeq 500 sequencing platform (Illumina, San Diego, CA).

Magnetic Cell Separation:

Article Title: Inhibition of methyltransferase Setd7 allows the in vitro expansion of myogenic stem cells with improved therapeutic potential
Article Snippet: Libraries were then indexed and PCR amplified (10 cycles) and sequenced using a NextSeq 500 High-Output Kit (Illumina) on a Illumina NextSeq 500 sequencing platform following the manufacturer’s protocols (Illumina). .. Libraries were then indexed and PCR amplified (10 cycles) and sequenced using a NextSeq 500 High-Output Kit (Illumina) on a Illumina NextSeq 500 sequencing platform following the manufacturer’s protocols (Illumina).

DNA Extraction:

Article Title: Alternative Growth Promoters Modulate Broiler Gut Microbiome and Enhance Body Weight Gain
Article Snippet: DNA extraction was carried out with QIAamp Fast DNA Stool Mini Kit (QIAGEN, Valencia, CA, United States) according to the manufacturer’s instructions. .. Paired-end sequencing (2 × 151 bp) was conducted on an Illumina NextSeq 500 sequencing platform with a NextSeq 500/550 v2 High Output flow cell.

Article Title: Fecal Metagenome Sequences from Lactating Dairy Cows Shedding Escherichia coli O157:H7
Article Snippet: Ten fecal samples collected from the lactating cows that were shedding E. coli O157:H7 were stored at −80°C , and the samples were later thawed for metagenome sequencing after DNA extraction using a QIAamp fast DNA stool minikit (Qiagen, Waltham, MA). .. Paired-end sequencing (2 × 151 bp) was conducted on an Illumina NextSeq 500 sequencing platform with a NextSeq 500/550 v2 high-output flow cell.

Article Title: Genomic and Evolutionary Analysis of Two Salmonella enterica Serovar Kentucky Sequence Types Isolated from Bovine and Poultry Sources in North America
Article Snippet: This inoculum was centrifuged, decanted, and then processed for DNA extraction using a Qiagen DNeasy Kit (Qiagen, Valencia, CA). .. Paired-end sequencing (2 X 151 bp) was conducted on an Illumina NextSeq 500 sequencing platform with a High-Output flow cell.

Nucleic Acid Electrophoresis:

Article Title: Circulating Exosomal miRNA Profile During Term and Preterm Birth Pregnancies: A Longitudinal Study
Article Snippet: The elution containing the pooled DNA library was further processed for cluster generation and sequencing using a NextSeq 500 high-output kit 75 cycles and Illumina NextSeq 500 sequencing platform, respectively. .. The elution containing the pooled DNA library was further processed for cluster generation and sequencing using a NextSeq 500 high-output kit 75 cycles and Illumina NextSeq 500 sequencing platform, respectively.

Article Title: Oxygen tension regulates the miRNA profile and bioactivity of exosomes released from extravillous trophoblast cells – Liquid biopsies for monitoring complications of pregnancy
Article Snippet: The elution containing the pooled DNA library was further processed for cluster generation and sequencing using NextSeq 500 High Output kit 75 cycles and Illumina NextSeq 500 sequencing platform, respectively. .. The elution containing the pooled DNA library was further processed for cluster generation and sequencing using NextSeq 500 High Output kit 75 cycles and Illumina NextSeq 500 sequencing platform, respectively.

RNA Sequencing Assay:

Article Title: Structural and functional adaptation of Haloferax volcanii TFEα/β
Article Snippet: Paragraph title: RNA-seq RNA isolation ... Sequencing was performed on the Illumina NextSeq 500 sequencing platform to generate 2 × 75 bp paired end reads using V2 chemistry.

Article Title: Emerin modulates spatial organization of chromosome territories in cells on softer matrices
Article Snippet: Cells were harvested in Trizol® reagent. .. RNA-Sequencing over two independent biological replicates was performed by Genotypic Technology, Bangalore on Illumina NextSeq 500 sequencing platform followed by quality control of paired end raw reads using FastQC v2.2. .. Reference genome alignment was performed using TopHat v2.0.7 and Cufflinks v2.0.1, and differential expression analysis was performed using Cufflinks v2.0.1—Cuffdiff ( ).

Article Title: Differential effects of FTY720 on the B cell compartment in a mouse model of multiple sclerosis
Article Snippet: Paragraph title: RNA-seq expression profiling ... Subsequently, all libraries were sequenced with a high output 1 × 75 cycles kit using the Illumina Nextseq 500 sequencing platform that generated 75 bp long single-end reads.

Article Title: Dedicated surveillance mechanism controls G-quadruplex forming non-coding RNAs in human mitochondria
Article Snippet: Sequencing (Figs and ) was carried out on an Illumina NextSeq 500 sequencing platform, using NextSeq 500 High Output Kit (150 cycles) (FC-404-1002, Illumina) and standard libraries denaturation and pair-end sequencing procedures of 2 × 75 cycles (Instructions: 15048776 Rev. .. Sequencing (Figs and ) was carried out on an Illumina NextSeq 500 sequencing platform, using NextSeq 500 High Output Kit (150 cycles) (FC-404-1002, Illumina) and standard libraries denaturation and pair-end sequencing procedures of 2 × 75 cycles (Instructions: 15048776 Rev.

Article Title: First case of B ALL with KMT2A-MAML2 rearrangement: a case report
Article Snippet: Total RNA was extracted from 1 × 107 cells using Trizol Reagent (life technologies). .. For paired-end RNA-Seq, we have used the TruSeq RNA Library Prep Kits followed by paired-end cluster generation and sequencing using the 2 × 150 NextSeq 500 high output kit and the Illumina NextSeq 500 sequencing platform. .. The first variant (Var 1 ) corresponded to the fusion of the exon 7 of KMT2A and the exon 2 of MAML2 as described previously [ ].

Article Title: Metastatic ability and the epithelial‐mesenchymal transition in induced cancer stem‐like hepatoma cells, et al. Metastatic ability and the epithelial‐mesenchymal transition in induced cancer stem‐like hepatoma cells
Article Snippet: Paragraph title: RNA sequencing ... Sequencing of paired‐end fragments (75 bp × 2) was carried out on a NextSeq 500 sequencing platform (Illumina) to a depth, in triplicate, of 123‐150 million fragments for SK‐HEP‐1 and SK‐sphere cells, and a depth, in duplicate, of 42‐46 million fragments for HuH‐7 cells.

Magnetic Beads:

Article Title: Inhibition of methyltransferase Setd7 allows the in vitro expansion of myogenic stem cells with improved therapeutic potential
Article Snippet: Histones were digested by Protease (ThermoFisher) for 30 minutes at 50° and DNA fragments were purified using Sera Mag magnetic beads in 30% PEG. .. Libraries were then indexed and PCR amplified (10 cycles) and sequenced using a NextSeq 500 High-Output Kit (Illumina) on a Illumina NextSeq 500 sequencing platform following the manufacturer’s protocols (Illumina).

Isolation:

Article Title: Structural and functional adaptation of Haloferax volcanii TFEα/β
Article Snippet: Paragraph title: RNA-seq RNA isolation ... Sequencing was performed on the Illumina NextSeq 500 sequencing platform to generate 2 × 75 bp paired end reads using V2 chemistry.

Article Title: Differential effects of FTY720 on the B cell compartment in a mouse model of multiple sclerosis
Article Snippet: RNA-seq expression profiling The complementary DNA (cDNA) libraries for all samples of isolated B cells were prepared using standard Illumina protocol, following the manufacturer’s instructions. .. Subsequently, all libraries were sequenced with a high output 1 × 75 cycles kit using the Illumina Nextseq 500 sequencing platform that generated 75 bp long single-end reads.

Article Title: First case of B ALL with KMT2A-MAML2 rearrangement: a case report
Article Snippet: Venous blood (10 mL) was collected in EDTA tubes from the patient and PBMC were isolated by erythrocytes lysis (Buffer EL, Qiagen). .. For paired-end RNA-Seq, we have used the TruSeq RNA Library Prep Kits followed by paired-end cluster generation and sequencing using the 2 × 150 NextSeq 500 high output kit and the Illumina NextSeq 500 sequencing platform.

Article Title: Metastatic ability and the epithelial‐mesenchymal transition in induced cancer stem‐like hepatoma cells, et al. Metastatic ability and the epithelial‐mesenchymal transition in induced cancer stem‐like hepatoma cells
Article Snippet: Total RNAs was isolated with the miRNeasy Mini Kit (Qiagen, Tokyo, Japan). .. Sequencing of paired‐end fragments (75 bp × 2) was carried out on a NextSeq 500 sequencing platform (Illumina) to a depth, in triplicate, of 123‐150 million fragments for SK‐HEP‐1 and SK‐sphere cells, and a depth, in duplicate, of 42‐46 million fragments for HuH‐7 cells.

Article Title: Versatile approach for functional analysis of human proteins and efficient stable cell line generation using FLP-mediated recombination system
Article Snippet: Paragraph title: RNA isolation, library construction and deep-sequencing ... Sequencing was carried out on an Illumina NextSeq 500 sequencing platform, using NextSeq 500 High Output Kit (150 cycles) (FC-404-1002, Illumina) and standard libraries denaturation and pair-end sequencing procedures (Instructions: 15048776 Rev.

Sequencing:

Article Title: Structural and functional adaptation of Haloferax volcanii TFEα/β
Article Snippet: Libraries were prepared using the dUTP method ( ) with NEBNext Ultra Directional RNA Library Prep kit for Illumina (New England Biolabs) and library quality was analyzed on a Bioanalyzer High-Sensitivity DNA-chip (Agilent Biotechnologies). .. Sequencing was performed on the Illumina NextSeq 500 sequencing platform to generate 2 × 75 bp paired end reads using V2 chemistry. .. Sequencing reads were trimmed to remove the adaptor parts using Trimmomatic ( ) and the quality of the sequencing data was assessed using FastQC ( http://www.bioinformatics.babraham.ac.uk/projects/fastqc ).

Article Title: Emerin modulates spatial organization of chromosome territories in cells on softer matrices
Article Snippet: Cells were harvested in Trizol® reagent. .. RNA-Sequencing over two independent biological replicates was performed by Genotypic Technology, Bangalore on Illumina NextSeq 500 sequencing platform followed by quality control of paired end raw reads using FastQC v2.2. .. Reference genome alignment was performed using TopHat v2.0.7 and Cufflinks v2.0.1, and differential expression analysis was performed using Cufflinks v2.0.1—Cuffdiff ( ).

Article Title: Differential effects of FTY720 on the B cell compartment in a mouse model of multiple sclerosis
Article Snippet: B cells were purified from the spleens of MP4-immunized mice using a commercially available Mouse B Cell Isolation Kit (Miltenyi Biotec, Bergisch Gladbach, Germany). .. Subsequently, all libraries were sequenced with a high output 1 × 75 cycles kit using the Illumina Nextseq 500 sequencing platform that generated 75 bp long single-end reads. .. The generated raw reads were processed using FastQC 0.11.3 for assessing read quality, amount of duplicates, and presence of adapter sequences.

Article Title: A mitogenomic timetree for Darwin’s enigmatic South American mammal Macrauchenia patachonica
Article Snippet: Sequencing of library and extraction blanks were included to check for the presence of contamination. .. For each library, we sequenced ∼2–20 million 75 bp PE read pairs on an Illumina Nextseq 500 sequencing platform. .. Raw Illumina intensity data were demultiplexed and converted to nucleotide sequences using the Illumina software, bcl2fastq.

Article Title: Circulating Exosomal miRNA Profile During Term and Preterm Birth Pregnancies: A Longitudinal Study
Article Snippet: Finally, the DNA libraries were eluted from the gel pieces overnight in 200 μL of nuclease-free H2 O. .. The elution containing the pooled DNA library was further processed for cluster generation and sequencing using a NextSeq 500 high-output kit 75 cycles and Illumina NextSeq 500 sequencing platform, respectively. .. The number of exosomes released from individuals are represented as mean ± SE (n = 20 for term and n = 10 for PTB, independent isolations from each patient from each trimester).

Article Title: Inhibition of methyltransferase Setd7 allows the in vitro expansion of myogenic stem cells with improved therapeutic potential
Article Snippet: Illumina sequencing libraries were generated by end repair, 3’ A-addition, and Illumina sequencing adaptor ligation (New England BioLabs, E6000B-10). .. Libraries were then indexed and PCR amplified (10 cycles) and sequenced using a NextSeq 500 High-Output Kit (Illumina) on a Illumina NextSeq 500 sequencing platform following the manufacturer’s protocols (Illumina). .. Sequence reads were mapped to mm10 (GRCm38) using Burrows-Wheeler Aligner (BWA) ( ).

Article Title: Oxygen tension regulates the miRNA profile and bioactivity of exosomes released from extravillous trophoblast cells – Liquid biopsies for monitoring complications of pregnancy
Article Snippet: Finally, the DNA libraries were eluted from the gel pieces overnight in 200 μL nuclease free H2 0. .. The elution containing the pooled DNA library was further processed for cluster generation and sequencing using NextSeq 500 High Output kit 75 cycles and Illumina NextSeq 500 sequencing platform, respectively. .. Initially, raw FASTQ files were processed to remove barcode and adaptor sequences.

Article Title: Genome-wide mapping of sister chromatid exchange events in single yeast cells using Strand-seq
Article Snippet: After ligation and PCR, DNA clean-up with magnetic AMPure XP beads (Agencourt AMPure, Beckman Coulter) was performed twice using a 1.2 vol of beads. .. Single-end 50 bp sequencing reads from the Strand-seq libraries were generated using the HiSeq 2500 or the NextSeq 500 sequencing platform (Illumina, San Diego, CA). .. Reads were aligned to the S. cerevisiae EF4 reference genome using Bowtie2 (version 2.0.0-beta7; ) and analyzed using the BAIT software package ( ) for data selection and visualization purposes.

Article Title: Daily changes in phytoplankton lipidomes reveal mechanisms of energy storage in the open ocean
Article Snippet: Purified total RNA was used to construct TruSeq cDNA libraries according to the Illumina TruSeq® RNA Sample Preparation v2 Guide. .. Briefly, RNAs were poly(A)-selected, converted to cDNAs, and sheared to ~225 bp fragments which were used to prepare cDNA libraries for paired-end (2 × 150) sequencing using the Illumina NextSeq 500 sequencing platform (Illumina Inc., San Diego, CA). .. Raw Illumina data were quality controlled with trimmomatic v0.36 using the parameters MAXINFO:135:0.5, LEADING:3, TRAILING:3, MINLEN:60, and AVGQUAL:20, and matching read pairs were merged using flash v1.2.11 with parameters -r 150 -f 250 -s 25.

Article Title: Alternative Growth Promoters Modulate Broiler Gut Microbiome and Enhance Body Weight Gain
Article Snippet: Nextera DNA libraries were made for each of the 20 samples separately using Nextera DNA Library Preparation Kit and Nextera Index Kit (Illumina, San Diego, CA, United States) followed by pooling into equimolar concentrations according to the manufacturer’s instructions. .. Paired-end sequencing (2 × 151 bp) was conducted on an Illumina NextSeq 500 sequencing platform with a NextSeq 500/550 v2 High Output flow cell. .. Metagenomic sequence data have been deposited at NCBI under accession numbers SRR5280289 , SRR5280393 , SRR5280514 , SRR5280757 , SRR5280758 , SRR5280759 , SRR5281813 , SRR5281814 , SRR5281815 , SRR5282096 , SRR5282097 , and SRR5282098 .

Article Title: RNA sequencing analysis of the developing chicken retina
Article Snippet: The resulting average size of the cDNA libraries was approximately 300 bp. .. Libraries for the 8 cDNA samples were sequenced using the Illumina NextSeq 500 sequencing platform yielding 28.6–72.2 million 125 bp paired end sequence reads per sample ( ; ). .. Between 28.7 and 72.2 million paired end sequence reads were obtained per sample from the Cold Spring Harbor Laboratory DNA Sequencing Center ( ).

Article Title: Dedicated surveillance mechanism controls G-quadruplex forming non-coding RNAs in human mitochondria
Article Snippet: B, 15035788 Rev. .. Sequencing (Figs and ) was carried out on an Illumina NextSeq 500 sequencing platform, using NextSeq 500 High Output Kit (150 cycles) (FC-404-1002, Illumina) and standard libraries denaturation and pair-end sequencing procedures of 2 × 75 cycles (Instructions: 15048776 Rev. .. D, 15046563 Rev.

Article Title: Fecal Metagenome Sequences from Lactating Dairy Cows Shedding Escherichia coli O157:H7
Article Snippet: Nextera DNA library preparation kits (Illumina, San Diego, CA) were used to prepare sequencing libraries of the 10 samples. .. Paired-end sequencing (2 × 151 bp) was conducted on an Illumina NextSeq 500 sequencing platform with a NextSeq 500/550 v2 high-output flow cell. .. Raw reads were demultiplexed using the BCL2FastQ program and cleaned using DeconSeq ( ) and Trimmomatic v0.36 ( ).

Article Title: First case of B ALL with KMT2A-MAML2 rearrangement: a case report
Article Snippet: Total RNA was extracted from 1 × 107 cells using Trizol Reagent (life technologies). .. For paired-end RNA-Seq, we have used the TruSeq RNA Library Prep Kits followed by paired-end cluster generation and sequencing using the 2 × 150 NextSeq 500 high output kit and the Illumina NextSeq 500 sequencing platform. .. The first variant (Var 1 ) corresponded to the fusion of the exon 7 of KMT2A and the exon 2 of MAML2 as described previously [ ].

Article Title: Evolution of tigecycline- and colistin-resistant CRKP (carbapenem-resistant Klebsiella pneumoniae) in vivo and its persistence in the GI tract
Article Snippet: STs were assigned using online database tools. .. Genomic sequencing was performed by means of the Illumina NextSeq 500 sequencing platform. .. Raw Illumina reads were trimmed or filtered to remove low-quality sequences and adapters, and assembled de novo by the SPAdes Genome Assembler v3.9.1 .

Article Title: Metastatic ability and the epithelial‐mesenchymal transition in induced cancer stem‐like hepatoma cells, et al. Metastatic ability and the epithelial‐mesenchymal transition in induced cancer stem‐like hepatoma cells
Article Snippet: Libraries were pooled after quantification by Bioanalyzer analysis as well as fluorometrically using Qubit dsDNA HS Assay kits and a Qubit 2.0 Fluorometer (Thermo Fisher Scientific). .. Sequencing of paired‐end fragments (75 bp × 2) was carried out on a NextSeq 500 sequencing platform (Illumina) to a depth, in triplicate, of 123‐150 million fragments for SK‐HEP‐1 and SK‐sphere cells, and a depth, in duplicate, of 42‐46 million fragments for HuH‐7 cells. .. Data for each sample were separated using unique barcode combinations and to generate FASTQ files.

Article Title: Versatile approach for functional analysis of human proteins and efficient stable cell line generation using FLP-mediated recombination system
Article Snippet: The libraries’ concentration was estimated by qPCR means with KAPA Universal Library Quantification Kit (KK4824, Kapa Biosystems), according to the supplied protocol. .. Sequencing was carried out on an Illumina NextSeq 500 sequencing platform, using NextSeq 500 High Output Kit (150 cycles) (FC-404-1002, Illumina) and standard libraries denaturation and pair-end sequencing procedures (Instructions: 15048776 Rev. .. D, 15046563 Rev.

Article Title: Genomic and Evolutionary Analysis of Two Salmonella enterica Serovar Kentucky Sequence Types Isolated from Bovine and Poultry Sources in North America
Article Snippet: Nextera XT libraries were made for each sample and pooled into equimolar concentrations following the manufacturer’s instructions (Illumina, San Diego, CA). .. Paired-end sequencing (2 X 151 bp) was conducted on an Illumina NextSeq 500 sequencing platform with a High-Output flow cell. .. Data were demultiplexed and trimmed to remove adaptor sequences using the BCL2FastQ program and PhiX reads were removed using DeconSeq [ ].

Labeling:

Article Title: Genomic and Evolutionary Analysis of Two Salmonella enterica Serovar Kentucky Sequence Types Isolated from Bovine and Poultry Sources in North America
Article Snippet: Paired-end sequencing (2 X 151 bp) was conducted on an Illumina NextSeq 500 sequencing platform with a High-Output flow cell. .. Paired-end sequencing (2 X 151 bp) was conducted on an Illumina NextSeq 500 sequencing platform with a High-Output flow cell.

Purification:

Article Title: Differential effects of FTY720 on the B cell compartment in a mouse model of multiple sclerosis
Article Snippet: B cells were purified from the spleens of MP4-immunized mice using a commercially available Mouse B Cell Isolation Kit (Miltenyi Biotec, Bergisch Gladbach, Germany). .. Subsequently, all libraries were sequenced with a high output 1 × 75 cycles kit using the Illumina Nextseq 500 sequencing platform that generated 75 bp long single-end reads.

Article Title: Inhibition of methyltransferase Setd7 allows the in vitro expansion of myogenic stem cells with improved therapeutic potential
Article Snippet: Histones were digested by Protease (ThermoFisher) for 30 minutes at 50° and DNA fragments were purified using Sera Mag magnetic beads in 30% PEG. .. Libraries were then indexed and PCR amplified (10 cycles) and sequenced using a NextSeq 500 High-Output Kit (Illumina) on a Illumina NextSeq 500 sequencing platform following the manufacturer’s protocols (Illumina).

Article Title: Daily changes in phytoplankton lipidomes reveal mechanisms of energy storage in the open ocean
Article Snippet: Purified total RNA was used to construct TruSeq cDNA libraries according to the Illumina TruSeq® RNA Sample Preparation v2 Guide. .. Briefly, RNAs were poly(A)-selected, converted to cDNAs, and sheared to ~225 bp fragments which were used to prepare cDNA libraries for paired-end (2 × 150) sequencing using the Illumina NextSeq 500 sequencing platform (Illumina Inc., San Diego, CA).

Article Title: Versatile approach for functional analysis of human proteins and efficient stable cell line generation using FLP-mediated recombination system
Article Snippet: TruSeq kit protocol temperature scheme with 12 amplification cycles and subsequent purification procedure was applied. .. Sequencing was carried out on an Illumina NextSeq 500 sequencing platform, using NextSeq 500 High Output Kit (150 cycles) (FC-404-1002, Illumina) and standard libraries denaturation and pair-end sequencing procedures (Instructions: 15048776 Rev.

Reverse Transcription Polymerase Chain Reaction:

Article Title: First case of B ALL with KMT2A-MAML2 rearrangement: a case report
Article Snippet: For paired-end RNA-Seq, we have used the TruSeq RNA Library Prep Kits followed by paired-end cluster generation and sequencing using the 2 × 150 NextSeq 500 high output kit and the Illumina NextSeq 500 sequencing platform. .. For the second variant (Var 2 ), the data revealed that the fusion point was within the exon 9 of KMT2A gene and within the intron 1–2 of MAML2 gene.

IA:

Article Title: Genomic and Evolutionary Analysis of Two Salmonella enterica Serovar Kentucky Sequence Types Isolated from Bovine and Poultry Sources in North America
Article Snippet: Isolates were serogrouped following the methods of Herrera-Leon et al. [ ]and Karns et al. [ ]and serotypes were identified by National Veterinary Services Laboratories (NVSL; Ames, IA). .. Paired-end sequencing (2 X 151 bp) was conducted on an Illumina NextSeq 500 sequencing platform with a High-Output flow cell.

Mouse Assay:

Article Title: Differential effects of FTY720 on the B cell compartment in a mouse model of multiple sclerosis
Article Snippet: B cells were purified from the spleens of MP4-immunized mice using a commercially available Mouse B Cell Isolation Kit (Miltenyi Biotec, Bergisch Gladbach, Germany). .. Subsequently, all libraries were sequenced with a high output 1 × 75 cycles kit using the Illumina Nextseq 500 sequencing platform that generated 75 bp long single-end reads.

Chromatin Immunoprecipitation:

Article Title: Inhibition of methyltransferase Setd7 allows the in vitro expansion of myogenic stem cells with improved therapeutic potential
Article Snippet: For ChIP, anti-H3K4me1 antibody (Diagenode, Cat# C15410037 ) was incubated with anti-IgA magnetic beads (Dynabeads, ThemoFisher) for 2 hours. .. Libraries were then indexed and PCR amplified (10 cycles) and sequenced using a NextSeq 500 High-Output Kit (Illumina) on a Illumina NextSeq 500 sequencing platform following the manufacturer’s protocols (Illumina).

Functional Assay:

Article Title: Fecal Metagenome Sequences from Lactating Dairy Cows Shedding Escherichia coli O157:H7
Article Snippet: Paired-end sequencing (2 × 151 bp) was conducted on an Illumina NextSeq 500 sequencing platform with a NextSeq 500/550 v2 high-output flow cell. .. Paired-end sequencing (2 × 151 bp) was conducted on an Illumina NextSeq 500 sequencing platform with a NextSeq 500/550 v2 high-output flow cell.

Sample Prep:

Article Title: Daily changes in phytoplankton lipidomes reveal mechanisms of energy storage in the open ocean
Article Snippet: Purified total RNA was used to construct TruSeq cDNA libraries according to the Illumina TruSeq® RNA Sample Preparation v2 Guide. .. Briefly, RNAs were poly(A)-selected, converted to cDNAs, and sheared to ~225 bp fragments which were used to prepare cDNA libraries for paired-end (2 × 150) sequencing using the Illumina NextSeq 500 sequencing platform (Illumina Inc., San Diego, CA).

Article Title: Metastatic ability and the epithelial‐mesenchymal transition in induced cancer stem‐like hepatoma cells, et al. Metastatic ability and the epithelial‐mesenchymal transition in induced cancer stem‐like hepatoma cells
Article Snippet: Sequencing libraries were constructed using the TruSeq Stranded Total RNA with Ribo‐Zero Gold LT Sample Prep kit (Illumina, Tokyo, Japan) according to manufacturer's instructions. .. Sequencing of paired‐end fragments (75 bp × 2) was carried out on a NextSeq 500 sequencing platform (Illumina) to a depth, in triplicate, of 123‐150 million fragments for SK‐HEP‐1 and SK‐sphere cells, and a depth, in duplicate, of 42‐46 million fragments for HuH‐7 cells.

Next-Generation Sequencing:

Article Title: Circulating Exosomal miRNA Profile During Term and Preterm Birth Pregnancies: A Longitudinal Study
Article Snippet: Paragraph title: Next-generation sequencing ... The elution containing the pooled DNA library was further processed for cluster generation and sequencing using a NextSeq 500 high-output kit 75 cycles and Illumina NextSeq 500 sequencing platform, respectively.

Article Title: Oxygen tension regulates the miRNA profile and bioactivity of exosomes released from extravillous trophoblast cells – Liquid biopsies for monitoring complications of pregnancy
Article Snippet: Paragraph title: Next generation sequencing ... The elution containing the pooled DNA library was further processed for cluster generation and sequencing using NextSeq 500 High Output kit 75 cycles and Illumina NextSeq 500 sequencing platform, respectively.

Article Title: RNA sequencing analysis of the developing chicken retina
Article Snippet: Total RNA samples chosen for characterization of global mRNA expression were submitted to the Cold Spring Harbor Laboratory DNA Sequencing Center for Bioanalyzer quality control analysis (Agilent) and Illumina Next Generation Sequencing. .. Libraries for the 8 cDNA samples were sequenced using the Illumina NextSeq 500 sequencing platform yielding 28.6–72.2 million 125 bp paired end sequence reads per sample ( ; ).

Article Title: Dedicated surveillance mechanism controls G-quadruplex forming non-coding RNAs in human mitochondria
Article Snippet: Paragraph title: Library preparation and next-generation sequencing ... Sequencing (Figs and ) was carried out on an Illumina NextSeq 500 sequencing platform, using NextSeq 500 High Output Kit (150 cycles) (FC-404-1002, Illumina) and standard libraries denaturation and pair-end sequencing procedures of 2 × 75 cycles (Instructions: 15048776 Rev.

Article Title: Metastatic ability and the epithelial‐mesenchymal transition in induced cancer stem‐like hepatoma cells, et al. Metastatic ability and the epithelial‐mesenchymal transition in induced cancer stem‐like hepatoma cells
Article Snippet: Sequencing of paired‐end fragments (75 bp × 2) was carried out on a NextSeq 500 sequencing platform (Illumina) to a depth, in triplicate, of 123‐150 million fragments for SK‐HEP‐1 and SK‐sphere cells, and a depth, in duplicate, of 42‐46 million fragments for HuH‐7 cells. .. Sequencing of paired‐end fragments (75 bp × 2) was carried out on a NextSeq 500 sequencing platform (Illumina) to a depth, in triplicate, of 123‐150 million fragments for SK‐HEP‐1 and SK‐sphere cells, and a depth, in duplicate, of 42‐46 million fragments for HuH‐7 cells.

Concentration Assay:

Article Title: Structural and functional adaptation of Haloferax volcanii TFEα/β
Article Snippet: Total RNA concentration was measured using Qubit RNA BR assay kit (Life technologies, Q10210). .. Sequencing was performed on the Illumina NextSeq 500 sequencing platform to generate 2 × 75 bp paired end reads using V2 chemistry.

Article Title: Versatile approach for functional analysis of human proteins and efficient stable cell line generation using FLP-mediated recombination system
Article Snippet: The libraries’ concentration was estimated by qPCR means with KAPA Universal Library Quantification Kit (KK4824, Kapa Biosystems), according to the supplied protocol. .. Sequencing was carried out on an Illumina NextSeq 500 sequencing platform, using NextSeq 500 High Output Kit (150 cycles) (FC-404-1002, Illumina) and standard libraries denaturation and pair-end sequencing procedures (Instructions: 15048776 Rev.

ChIP-sequencing:

Article Title: Inhibition of methyltransferase Setd7 allows the in vitro expansion of myogenic stem cells with improved therapeutic potential
Article Snippet: Paragraph title: ChIP-Seq methods and analysis ... Libraries were then indexed and PCR amplified (10 cycles) and sequenced using a NextSeq 500 High-Output Kit (Illumina) on a Illumina NextSeq 500 sequencing platform following the manufacturer’s protocols (Illumina).

Lysis:

Article Title: First case of B ALL with KMT2A-MAML2 rearrangement: a case report
Article Snippet: Venous blood (10 mL) was collected in EDTA tubes from the patient and PBMC were isolated by erythrocytes lysis (Buffer EL, Qiagen). .. For paired-end RNA-Seq, we have used the TruSeq RNA Library Prep Kits followed by paired-end cluster generation and sequencing using the 2 × 150 NextSeq 500 high output kit and the Illumina NextSeq 500 sequencing platform.

Variant Assay:

Article Title: First case of B ALL with KMT2A-MAML2 rearrangement: a case report
Article Snippet: For paired-end RNA-Seq, we have used the TruSeq RNA Library Prep Kits followed by paired-end cluster generation and sequencing using the 2 × 150 NextSeq 500 high output kit and the Illumina NextSeq 500 sequencing platform. .. The first variant (Var 1 ) corresponded to the fusion of the exon 7 of KMT2A and the exon 2 of MAML2 as described previously [ ].

Fluorescence In Situ Hybridization:

Article Title: First case of B ALL with KMT2A-MAML2 rearrangement: a case report
Article Snippet: FISH analysis was performed on bone marrow aspirate (Fig. ) using a LSI KMT2A break apart probe (Vysis, Abbott Molecular Inc.). .. For paired-end RNA-Seq, we have used the TruSeq RNA Library Prep Kits followed by paired-end cluster generation and sequencing using the 2 × 150 NextSeq 500 high output kit and the Illumina NextSeq 500 sequencing platform.

Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 97
    Illumina Inc nextseq 500 sequencing platform
    Nextseq 500 Sequencing Platform, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 97/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nextseq 500 sequencing platform/product/Illumina Inc
    Average 97 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    nextseq 500 sequencing platform - by Bioz Stars, 2019-12
    97/100 stars
      Buy from Supplier

    99
    Illumina Inc sequencing platform
    Sequencing Platform, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 948 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sequencing platform/product/Illumina Inc
    Average 99 stars, based on 948 article reviews
    Price from $9.99 to $1999.99
    sequencing platform - by Bioz Stars, 2019-12
    99/100 stars
      Buy from Supplier

    Image Search Results