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Illumina Inc nextera index oligos
Nextera Index Oligos, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/nextera index oligos/product/Illumina Inc
Average 94 stars, based on 1 article reviews
Price from $9.99 to $1999.99
nextera index oligos - by Bioz Stars, 2020-04
94/100 stars

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Polymerase Chain Reaction:

Article Title: Plant lamin-like proteins mediate chromatin tethering at the nuclear periphery
Article Snippet: .. The transposed DNA was purified with MinElute PCR Purification Kit (Qiagen) and amplified with selected Nextera index oligos (Illumina). .. Size selection of PCR products was performed with AMPure® XP beads (Beckman Coulter) to collect library molecules between 200 and 600 bp.

Amplification:

Article Title: Plant lamin-like proteins mediate chromatin tethering at the nuclear periphery
Article Snippet: .. The transposed DNA was purified with MinElute PCR Purification Kit (Qiagen) and amplified with selected Nextera index oligos (Illumina). .. Size selection of PCR products was performed with AMPure® XP beads (Beckman Coulter) to collect library molecules between 200 and 600 bp.

Selection:

Article Title: Plant lamin-like proteins mediate chromatin tethering at the nuclear periphery
Article Snippet: The transposed DNA was purified with MinElute PCR Purification Kit (Qiagen) and amplified with selected Nextera index oligos (Illumina). .. Size selection of PCR products was performed with AMPure® XP beads (Beckman Coulter) to collect library molecules between 200 and 600 bp.

Purification:

Article Title: Plant lamin-like proteins mediate chromatin tethering at the nuclear periphery
Article Snippet: .. The transposed DNA was purified with MinElute PCR Purification Kit (Qiagen) and amplified with selected Nextera index oligos (Illumina). .. Size selection of PCR products was performed with AMPure® XP beads (Beckman Coulter) to collect library molecules between 200 and 600 bp.

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  • 99
    Illumina Inc nextera index primers
    NGS-TILLING process. A : A long-term resource for many TILLING screens consisting of a genomic DNA sample and a corresponding cryopreserved sperm sample was prepared from 9,024 F1 ENU-mutagenized male zebrafish. B : Library Pooling. Normalized genomic DNA (gDNA) was pooled twice: first, gDNA from 6 fish was pooled together to make 1,504 6-fish pools in 16 96-well plates. These six-fish pools will be used for HRM identification of carrier fish (step F ). Second, groups of 48 6-fish pools were pooled together into 288-fish pools (a total of 32 288-fish pools). C : Target Preparation. gDNA from 288-fish pools was used as a template for PCR amplification of ~250 bp fragments corresponding to exons of genes of interest using gene-specific primers with P5/P7 SEQ tails (green). After normalization, amplicons from each 288-fish pool were combined and used as template for a brief second PCR that added <t>Nextera</t> index sequences (blue) and Illumina P5/P7 sequences (yellow). D : Sequencing: All amplicons from the entire library were combined and sequenced (Illumina MiSeq platform), generating fully overlapping 250 bp paired-end sequences. E : Data Analysis. Sequence analysis using PELE and PoDATA identified rare deleterious variants (occurring in 1/100 to 1/1000 reads) in single 288-fish pools. F : Deconvolution. A fragment centered on a putative variant call was amplified from each of the 48-six-fish pools used to make up the 288-fish pool in which that variant was detected, and was subjected to High Resolution Melt (HRM) Analysis. Then HRM of the six individual fish in the six-fish pool that showed distinct melting kinetics identified the individual carrier. G : Mutant Recovery. Finally, the presence of the variant identified by PELE and PoDATA was confirmed in that fish by Sanger sequencing. F2 heterozygotes were generated by in vitro fertilization of WT eggs with the corresponding cryopreserved sperm sample.
    Nextera Index Primers, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nextera index primers/product/Illumina Inc
    Average 99 stars, based on 18 article reviews
    Price from $9.99 to $1999.99
    nextera index primers - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    94
    Illumina Inc nextera xt 384 way indexing primers
    NGS-TILLING process. A : A long-term resource for many TILLING screens consisting of a genomic DNA sample and a corresponding cryopreserved sperm sample was prepared from 9,024 F1 ENU-mutagenized male zebrafish. B : Library Pooling. Normalized genomic DNA (gDNA) was pooled twice: first, gDNA from 6 fish was pooled together to make 1,504 6-fish pools in 16 96-well plates. These six-fish pools will be used for HRM identification of carrier fish (step F ). Second, groups of 48 6-fish pools were pooled together into 288-fish pools (a total of 32 288-fish pools). C : Target Preparation. gDNA from 288-fish pools was used as a template for PCR amplification of ~250 bp fragments corresponding to exons of genes of interest using gene-specific primers with P5/P7 SEQ tails (green). After normalization, amplicons from each 288-fish pool were combined and used as template for a brief second PCR that added <t>Nextera</t> index sequences (blue) and Illumina P5/P7 sequences (yellow). D : Sequencing: All amplicons from the entire library were combined and sequenced (Illumina MiSeq platform), generating fully overlapping 250 bp paired-end sequences. E : Data Analysis. Sequence analysis using PELE and PoDATA identified rare deleterious variants (occurring in 1/100 to 1/1000 reads) in single 288-fish pools. F : Deconvolution. A fragment centered on a putative variant call was amplified from each of the 48-six-fish pools used to make up the 288-fish pool in which that variant was detected, and was subjected to High Resolution Melt (HRM) Analysis. Then HRM of the six individual fish in the six-fish pool that showed distinct melting kinetics identified the individual carrier. G : Mutant Recovery. Finally, the presence of the variant identified by PELE and PoDATA was confirmed in that fish by Sanger sequencing. F2 heterozygotes were generated by in vitro fertilization of WT eggs with the corresponding cryopreserved sperm sample.
    Nextera Xt 384 Way Indexing Primers, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nextera xt 384 way indexing primers/product/Illumina Inc
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    nextera xt 384 way indexing primers - by Bioz Stars, 2020-04
    94/100 stars
      Buy from Supplier

    95
    Illumina Inc sequencing adapters nextera xt index primer
    NGS-TILLING process. A : A long-term resource for many TILLING screens consisting of a genomic DNA sample and a corresponding cryopreserved sperm sample was prepared from 9,024 F1 ENU-mutagenized male zebrafish. B : Library Pooling. Normalized genomic DNA (gDNA) was pooled twice: first, gDNA from 6 fish was pooled together to make 1,504 6-fish pools in 16 96-well plates. These six-fish pools will be used for HRM identification of carrier fish (step F ). Second, groups of 48 6-fish pools were pooled together into 288-fish pools (a total of 32 288-fish pools). C : Target Preparation. gDNA from 288-fish pools was used as a template for PCR amplification of ~250 bp fragments corresponding to exons of genes of interest using gene-specific primers with P5/P7 SEQ tails (green). After normalization, amplicons from each 288-fish pool were combined and used as template for a brief second PCR that added <t>Nextera</t> index sequences (blue) and Illumina P5/P7 sequences (yellow). D : Sequencing: All amplicons from the entire library were combined and sequenced (Illumina MiSeq platform), generating fully overlapping 250 bp paired-end sequences. E : Data Analysis. Sequence analysis using PELE and PoDATA identified rare deleterious variants (occurring in 1/100 to 1/1000 reads) in single 288-fish pools. F : Deconvolution. A fragment centered on a putative variant call was amplified from each of the 48-six-fish pools used to make up the 288-fish pool in which that variant was detected, and was subjected to High Resolution Melt (HRM) Analysis. Then HRM of the six individual fish in the six-fish pool that showed distinct melting kinetics identified the individual carrier. G : Mutant Recovery. Finally, the presence of the variant identified by PELE and PoDATA was confirmed in that fish by Sanger sequencing. F2 heterozygotes were generated by in vitro fertilization of WT eggs with the corresponding cryopreserved sperm sample.
    Sequencing Adapters Nextera Xt Index Primer, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 95/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sequencing adapters nextera xt index primer/product/Illumina Inc
    Average 95 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    sequencing adapters nextera xt index primer - by Bioz Stars, 2020-04
    95/100 stars
      Buy from Supplier

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    NGS-TILLING process. A : A long-term resource for many TILLING screens consisting of a genomic DNA sample and a corresponding cryopreserved sperm sample was prepared from 9,024 F1 ENU-mutagenized male zebrafish. B : Library Pooling. Normalized genomic DNA (gDNA) was pooled twice: first, gDNA from 6 fish was pooled together to make 1,504 6-fish pools in 16 96-well plates. These six-fish pools will be used for HRM identification of carrier fish (step F ). Second, groups of 48 6-fish pools were pooled together into 288-fish pools (a total of 32 288-fish pools). C : Target Preparation. gDNA from 288-fish pools was used as a template for PCR amplification of ~250 bp fragments corresponding to exons of genes of interest using gene-specific primers with P5/P7 SEQ tails (green). After normalization, amplicons from each 288-fish pool were combined and used as template for a brief second PCR that added Nextera index sequences (blue) and Illumina P5/P7 sequences (yellow). D : Sequencing: All amplicons from the entire library were combined and sequenced (Illumina MiSeq platform), generating fully overlapping 250 bp paired-end sequences. E : Data Analysis. Sequence analysis using PELE and PoDATA identified rare deleterious variants (occurring in 1/100 to 1/1000 reads) in single 288-fish pools. F : Deconvolution. A fragment centered on a putative variant call was amplified from each of the 48-six-fish pools used to make up the 288-fish pool in which that variant was detected, and was subjected to High Resolution Melt (HRM) Analysis. Then HRM of the six individual fish in the six-fish pool that showed distinct melting kinetics identified the individual carrier. G : Mutant Recovery. Finally, the presence of the variant identified by PELE and PoDATA was confirmed in that fish by Sanger sequencing. F2 heterozygotes were generated by in vitro fertilization of WT eggs with the corresponding cryopreserved sperm sample.

    Journal: BMC Genomics

    Article Title: Rapid identification and recovery of ENU-induced mutations with next-generation sequencing and Paired-End Low-Error analysis

    doi: 10.1186/s12864-015-1263-4

    Figure Lengend Snippet: NGS-TILLING process. A : A long-term resource for many TILLING screens consisting of a genomic DNA sample and a corresponding cryopreserved sperm sample was prepared from 9,024 F1 ENU-mutagenized male zebrafish. B : Library Pooling. Normalized genomic DNA (gDNA) was pooled twice: first, gDNA from 6 fish was pooled together to make 1,504 6-fish pools in 16 96-well plates. These six-fish pools will be used for HRM identification of carrier fish (step F ). Second, groups of 48 6-fish pools were pooled together into 288-fish pools (a total of 32 288-fish pools). C : Target Preparation. gDNA from 288-fish pools was used as a template for PCR amplification of ~250 bp fragments corresponding to exons of genes of interest using gene-specific primers with P5/P7 SEQ tails (green). After normalization, amplicons from each 288-fish pool were combined and used as template for a brief second PCR that added Nextera index sequences (blue) and Illumina P5/P7 sequences (yellow). D : Sequencing: All amplicons from the entire library were combined and sequenced (Illumina MiSeq platform), generating fully overlapping 250 bp paired-end sequences. E : Data Analysis. Sequence analysis using PELE and PoDATA identified rare deleterious variants (occurring in 1/100 to 1/1000 reads) in single 288-fish pools. F : Deconvolution. A fragment centered on a putative variant call was amplified from each of the 48-six-fish pools used to make up the 288-fish pool in which that variant was detected, and was subjected to High Resolution Melt (HRM) Analysis. Then HRM of the six individual fish in the six-fish pool that showed distinct melting kinetics identified the individual carrier. G : Mutant Recovery. Finally, the presence of the variant identified by PELE and PoDATA was confirmed in that fish by Sanger sequencing. F2 heterozygotes were generated by in vitro fertilization of WT eggs with the corresponding cryopreserved sperm sample.

    Article Snippet: Equal amounts of each of the 25 gene-specific PCR products from each 288-fish pool were combined and briefly amplified (5 cycles) using Illumina Nextera index primers to add a pair of specific Illumina indices and P5/P7 tail to the amplicons from each pool (Figure C).

    Techniques: Next-Generation Sequencing, Fluorescence In Situ Hybridization, Polymerase Chain Reaction, Amplification, Sequencing, Variant Assay, Mutagenesis, Generated, In Vitro