Structured Review

Ventana Medical nexes stainer
Nexes Stainer, supplied by Ventana Medical, used in various techniques. Bioz Stars score: 83/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/nexes stainer/product/Ventana Medical
Average 83 stars, based on 3 article reviews
Price from $9.99 to $1999.99
nexes stainer - by Bioz Stars, 2020-02
83/100 stars

Related Products / Commonly Used Together

immunodetection
immunolabeling
erbb2
cleaved caspase-3
ki67

Images

Related Articles

Immunohistochemistry:

Article Title: HER2 status in gastro-oesophageal adenocarcinomas assessed by two rabbit monoclonal antibodies (SP3 and 4B5) and two in situ hybridization methods (FISH and SISH)
Article Snippet: .. HER2 IHC studies were carried out on the NeXes stainer (Ventana) using 3-μm slides after antigen retrieval (10 mm citrate, pH 7.3, boiling time 25 min). ..

Article Title: Overcoming IGF1R/IR Resistance Through Inhibition of MEK Signaling in Colorectal Cancer Models
Article Snippet: Paragraph title: Immunohistochemistry ... Immunodetection was performed on the NexES stainer (Ventana Medical Systems, Tucson, AZ) at an operating temperature of 37°C with a primary antibody incubation time of 32 minutes.

Article Title: Therapeutic targeting of erbB3 with MM-121/SAR256212 enhances antitumor activity of paclitaxel against erbB2-overexpressing breast cancer
Article Snippet: Paragraph title: Immunohistochemistry (IHC) ... Immunodetection of Ki-67, cleaved caspase-3 and erbB2 was performed on the NexES stainer (Ventana) at an operating temperature of 37°C.

Article Title: Intrinsic circannual regulation of brown adipose tissue form and function in tune with hibernation
Article Snippet: Five-micrometer sections from formalin-perfused SpT ( n = 2), SA, FT, and interbout arousal (IBA) (all n = 3) were deparaffinized, then processed for immunohistochemical detection of Ki-67. .. Immunolabeling of Ki-67 (1:500 rabbit mAb SP6, no. RM-9106-SO; Neomarkers/Pierce, Rockford, IL) was accomplished following a 32-min incubation with primary Ab at 37°C on a NexES stainer (Ventana Medical Systems, Tucson, AZ).

Article Title: The anti-erbB3 antibody MM-121/SAR256212 in combination with trastuzumab exerts potent antitumor activity against trastuzumab-resistant breast cancer cells
Article Snippet: Paragraph title: Immunohistochemistry ... Immunodetection of Ki67, cleaved Caspase-3 and erbB2 was performed on the NexES stainer (Ventana Medical Systems, Tucson, AZ) at an operating temperature of 37°C.

Sample Prep:

Article Title: Intrinsic circannual regulation of brown adipose tissue form and function in tune with hibernation
Article Snippet: Paragraph title: Histology sample preparation and analysis. ... Immunolabeling of Ki-67 (1:500 rabbit mAb SP6, no. RM-9106-SO; Neomarkers/Pierce, Rockford, IL) was accomplished following a 32-min incubation with primary Ab at 37°C on a NexES stainer (Ventana Medical Systems, Tucson, AZ).

Single-particle Tracking:

Article Title: Intrinsic circannual regulation of brown adipose tissue form and function in tune with hibernation
Article Snippet: Five-micrometer sections from formalin-perfused SpT ( n = 2), SA, FT, and interbout arousal (IBA) (all n = 3) were deparaffinized, then processed for immunohistochemical detection of Ki-67. .. Immunolabeling of Ki-67 (1:500 rabbit mAb SP6, no. RM-9106-SO; Neomarkers/Pierce, Rockford, IL) was accomplished following a 32-min incubation with primary Ab at 37°C on a NexES stainer (Ventana Medical Systems, Tucson, AZ).

Positive Control:

Article Title: Therapeutic targeting of erbB3 with MM-121/SAR256212 enhances antitumor activity of paclitaxel against erbB2-overexpressing breast cancer
Article Snippet: For Ki-67 and cleaved caspase-3, human tonsil tissues were used a positive control. .. Immunodetection of Ki-67, cleaved caspase-3 and erbB2 was performed on the NexES stainer (Ventana) at an operating temperature of 37°C.

Article Title: The anti-erbB3 antibody MM-121/SAR256212 in combination with trastuzumab exerts potent antitumor activity against trastuzumab-resistant breast cancer cells
Article Snippet: For Ki67 and cleaved caspase-3, the human tonsil tissues were used a positive control. .. Immunodetection of Ki67, cleaved Caspase-3 and erbB2 was performed on the NexES stainer (Ventana Medical Systems, Tucson, AZ) at an operating temperature of 37°C.

Immunolabeling:

Article Title: Intrinsic circannual regulation of brown adipose tissue form and function in tune with hibernation
Article Snippet: .. Immunolabeling of Ki-67 (1:500 rabbit mAb SP6, no. RM-9106-SO; Neomarkers/Pierce, Rockford, IL) was accomplished following a 32-min incubation with primary Ab at 37°C on a NexES stainer (Ventana Medical Systems, Tucson, AZ). .. Secondary detection employed a modification of the I-VIEW DAB detection kit (Ventana); specifically, the I-VIEW secondary antibody and enzyme were replaced with Rabbit ImmPress no. MP-7401 (Vector Laboratories, Burlingame, CA).

Immunodetection:

Article Title: Overcoming IGF1R/IR Resistance Through Inhibition of MEK Signaling in Colorectal Cancer Models
Article Snippet: .. Immunodetection was performed on the NexES stainer (Ventana Medical Systems, Tucson, AZ) at an operating temperature of 37°C with a primary antibody incubation time of 32 minutes. .. Antibodies were detected with a modified I-VIEW DAB (Ventana) detection kit.

Article Title: Therapeutic targeting of erbB3 with MM-121/SAR256212 enhances antitumor activity of paclitaxel against erbB2-overexpressing breast cancer
Article Snippet: .. Immunodetection of Ki-67, cleaved caspase-3 and erbB2 was performed on the NexES stainer (Ventana) at an operating temperature of 37°C. .. Ki-67 and cleaved caspase-3 antibodies were incubated for 32 minutes and detected with a modified I-VIEW DAB detection kit (Ventana).

Article Title: The anti-erbB3 antibody MM-121/SAR256212 in combination with trastuzumab exerts potent antitumor activity against trastuzumab-resistant breast cancer cells
Article Snippet: .. Immunodetection of Ki67, cleaved Caspase-3 and erbB2 was performed on the NexES stainer (Ventana Medical Systems, Tucson, AZ) at an operating temperature of 37°C. .. Ki67 and cleaved caspase-3 antibodies were incubated for 32 min and detected with a modified I-VIEW DAB (Ventana) detection kit.

Incubation:

Article Title: HER2 status in gastro-oesophageal adenocarcinomas assessed by two rabbit monoclonal antibodies (SP3 and 4B5) and two in situ hybridization methods (FISH and SISH)
Article Snippet: HER2 IHC studies were carried out on the NeXes stainer (Ventana) using 3-μm slides after antigen retrieval (10 mm citrate, pH 7.3, boiling time 25 min). .. The rabbit monoclonal antibody SP3 was used at a dilution of 1:20 with a 32-min incubation time at 37°C.

Article Title: Overcoming IGF1R/IR Resistance Through Inhibition of MEK Signaling in Colorectal Cancer Models
Article Snippet: .. Immunodetection was performed on the NexES stainer (Ventana Medical Systems, Tucson, AZ) at an operating temperature of 37°C with a primary antibody incubation time of 32 minutes. .. Antibodies were detected with a modified I-VIEW DAB (Ventana) detection kit.

Article Title: Therapeutic targeting of erbB3 with MM-121/SAR256212 enhances antitumor activity of paclitaxel against erbB2-overexpressing breast cancer
Article Snippet: Immunodetection of Ki-67, cleaved caspase-3 and erbB2 was performed on the NexES stainer (Ventana) at an operating temperature of 37°C. .. Ki-67 and cleaved caspase-3 antibodies were incubated for 32 minutes and detected with a modified I-VIEW DAB detection kit (Ventana).

Article Title: Intrinsic circannual regulation of brown adipose tissue form and function in tune with hibernation
Article Snippet: .. Immunolabeling of Ki-67 (1:500 rabbit mAb SP6, no. RM-9106-SO; Neomarkers/Pierce, Rockford, IL) was accomplished following a 32-min incubation with primary Ab at 37°C on a NexES stainer (Ventana Medical Systems, Tucson, AZ). .. Secondary detection employed a modification of the I-VIEW DAB detection kit (Ventana); specifically, the I-VIEW secondary antibody and enzyme were replaced with Rabbit ImmPress no. MP-7401 (Vector Laboratories, Burlingame, CA).

Article Title: The anti-erbB3 antibody MM-121/SAR256212 in combination with trastuzumab exerts potent antitumor activity against trastuzumab-resistant breast cancer cells
Article Snippet: Immunodetection of Ki67, cleaved Caspase-3 and erbB2 was performed on the NexES stainer (Ventana Medical Systems, Tucson, AZ) at an operating temperature of 37°C. .. Ki67 and cleaved caspase-3 antibodies were incubated for 32 min and detected with a modified I-VIEW DAB (Ventana) detection kit.

Expressing:

Article Title: Therapeutic targeting of erbB3 with MM-121/SAR256212 enhances antitumor activity of paclitaxel against erbB2-overexpressing breast cancer
Article Snippet: For the negative controls, in addition to use the same cells/tissues without addition of the primary antibodies, we also performed the IHC assays using the tissues or cell lines that are known to have no expression of the antigens (Additional file ). .. Immunodetection of Ki-67, cleaved caspase-3 and erbB2 was performed on the NexES stainer (Ventana) at an operating temperature of 37°C.

Modification:

Article Title: Overcoming IGF1R/IR Resistance Through Inhibition of MEK Signaling in Colorectal Cancer Models
Article Snippet: Immunodetection was performed on the NexES stainer (Ventana Medical Systems, Tucson, AZ) at an operating temperature of 37°C with a primary antibody incubation time of 32 minutes. .. Antibodies were detected with a modified I-VIEW DAB (Ventana) detection kit.

Article Title: Therapeutic targeting of erbB3 with MM-121/SAR256212 enhances antitumor activity of paclitaxel against erbB2-overexpressing breast cancer
Article Snippet: Immunodetection of Ki-67, cleaved caspase-3 and erbB2 was performed on the NexES stainer (Ventana) at an operating temperature of 37°C. .. Ki-67 and cleaved caspase-3 antibodies were incubated for 32 minutes and detected with a modified I-VIEW DAB detection kit (Ventana).

Article Title: Intrinsic circannual regulation of brown adipose tissue form and function in tune with hibernation
Article Snippet: Immunolabeling of Ki-67 (1:500 rabbit mAb SP6, no. RM-9106-SO; Neomarkers/Pierce, Rockford, IL) was accomplished following a 32-min incubation with primary Ab at 37°C on a NexES stainer (Ventana Medical Systems, Tucson, AZ). .. Secondary detection employed a modification of the I-VIEW DAB detection kit (Ventana); specifically, the I-VIEW secondary antibody and enzyme were replaced with Rabbit ImmPress no. MP-7401 (Vector Laboratories, Burlingame, CA).

Article Title: The anti-erbB3 antibody MM-121/SAR256212 in combination with trastuzumab exerts potent antitumor activity against trastuzumab-resistant breast cancer cells
Article Snippet: Immunodetection of Ki67, cleaved Caspase-3 and erbB2 was performed on the NexES stainer (Ventana Medical Systems, Tucson, AZ) at an operating temperature of 37°C. .. Ki67 and cleaved caspase-3 antibodies were incubated for 32 min and detected with a modified I-VIEW DAB (Ventana) detection kit.

Staining:

Article Title: HER2 status in gastro-oesophageal adenocarcinomas assessed by two rabbit monoclonal antibodies (SP3 and 4B5) and two in situ hybridization methods (FISH and SISH)
Article Snippet: On the newly cut slides stained with haematoxylin and eosin (H & E), the tumour was typed as ‘intestinal’, ‘diffuse’, ‘mucinous’ or ‘mixed’ using the Laurén classification. .. HER2 IHC studies were carried out on the NeXes stainer (Ventana) using 3-μm slides after antigen retrieval (10 mm citrate, pH 7.3, boiling time 25 min).

Article Title: Overcoming IGF1R/IR Resistance Through Inhibition of MEK Signaling in Colorectal Cancer Models
Article Snippet: Five micron thick paraffin sections were deparaffinized, antigens unmasked and immunohistochemically stained for Ki-67 (Neomarkers/Thermo Scientific, Waltham, MA; rabbit monoclonal SP6; cat# RM-9106-SO; dilution 1:500 in TBST + 1% BSA w/v). .. Immunodetection was performed on the NexES stainer (Ventana Medical Systems, Tucson, AZ) at an operating temperature of 37°C with a primary antibody incubation time of 32 minutes.

Article Title: Intrinsic circannual regulation of brown adipose tissue form and function in tune with hibernation
Article Snippet: Ten-micrometer sections were placed on glass slides, air dried, and then stained with hematoxylin. .. Immunolabeling of Ki-67 (1:500 rabbit mAb SP6, no. RM-9106-SO; Neomarkers/Pierce, Rockford, IL) was accomplished following a 32-min incubation with primary Ab at 37°C on a NexES stainer (Ventana Medical Systems, Tucson, AZ).

Plasmid Preparation:

Article Title: Overcoming IGF1R/IR Resistance Through Inhibition of MEK Signaling in Colorectal Cancer Models
Article Snippet: Immunodetection was performed on the NexES stainer (Ventana Medical Systems, Tucson, AZ) at an operating temperature of 37°C with a primary antibody incubation time of 32 minutes. .. Immunodetection was performed on the NexES stainer (Ventana Medical Systems, Tucson, AZ) at an operating temperature of 37°C with a primary antibody incubation time of 32 minutes.

Article Title: Therapeutic targeting of erbB3 with MM-121/SAR256212 enhances antitumor activity of paclitaxel against erbB2-overexpressing breast cancer
Article Snippet: Immunodetection of Ki-67, cleaved caspase-3 and erbB2 was performed on the NexES stainer (Ventana) at an operating temperature of 37°C. .. Immunodetection of Ki-67, cleaved caspase-3 and erbB2 was performed on the NexES stainer (Ventana) at an operating temperature of 37°C.

Article Title: Intrinsic circannual regulation of brown adipose tissue form and function in tune with hibernation
Article Snippet: Immunolabeling of Ki-67 (1:500 rabbit mAb SP6, no. RM-9106-SO; Neomarkers/Pierce, Rockford, IL) was accomplished following a 32-min incubation with primary Ab at 37°C on a NexES stainer (Ventana Medical Systems, Tucson, AZ). .. Secondary detection employed a modification of the I-VIEW DAB detection kit (Ventana); specifically, the I-VIEW secondary antibody and enzyme were replaced with Rabbit ImmPress no. MP-7401 (Vector Laboratories, Burlingame, CA).

Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 77
    Ventana Medical immunostaining station
    FE65 <t>immunostaining.</t> A: Western blot of FE65 immunoreactivity in human brain homogenates ( left , Non Abs. column). Absorption of the antibody (Abs. column) strongly diminished immunoreactivity. Molecular size markers are shown on the left (kd). On human brain paraffin-embedded sections, absorption of the antibody leads to similar results [compare “Non absorbed” ( middle ) and “Absorbed” ( right )]. B: FE65 immunoreactivity was observed in AD and control cases in all types of neurons such as pyramidal cells of the hippocampus as illustrated on this photograph. Neuronal nuclei were devoid of immunostained material whereas a dense cytoplasmic FE65 immunoreactivity was noted. Scale bar, 50 μm. C: Proportion of FE65 immunoreactivity (mean ± SEM) in allo- ( left ) and isocortical ( right ) samples in control (C), AD with Braak stage IV (AD IV), and AD with Braak stage VI (AD VI) cases. FE65 immunoreactivity in the hippocampus (CA4) was higher in AD cases with severe neuropathological lesions as compared to patients with less advanced pathology and nondemented/age-matched cases. No differences were observed in the isocortex between AD and control cases.
    Immunostaining Station, supplied by Ventana Medical, used in various techniques. Bioz Stars score: 77/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/immunostaining station/product/Ventana Medical
    Average 77 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    immunostaining station - by Bioz Stars, 2020-02
    77/100 stars
      Buy from Supplier

    Image Search Results


    FE65 immunostaining. A: Western blot of FE65 immunoreactivity in human brain homogenates ( left , Non Abs. column). Absorption of the antibody (Abs. column) strongly diminished immunoreactivity. Molecular size markers are shown on the left (kd). On human brain paraffin-embedded sections, absorption of the antibody leads to similar results [compare “Non absorbed” ( middle ) and “Absorbed” ( right )]. B: FE65 immunoreactivity was observed in AD and control cases in all types of neurons such as pyramidal cells of the hippocampus as illustrated on this photograph. Neuronal nuclei were devoid of immunostained material whereas a dense cytoplasmic FE65 immunoreactivity was noted. Scale bar, 50 μm. C: Proportion of FE65 immunoreactivity (mean ± SEM) in allo- ( left ) and isocortical ( right ) samples in control (C), AD with Braak stage IV (AD IV), and AD with Braak stage VI (AD VI) cases. FE65 immunoreactivity in the hippocampus (CA4) was higher in AD cases with severe neuropathological lesions as compared to patients with less advanced pathology and nondemented/age-matched cases. No differences were observed in the isocortex between AD and control cases.

    Journal: The American Journal of Pathology

    Article Title: FE65 in Alzheimer's Disease

    doi:

    Figure Lengend Snippet: FE65 immunostaining. A: Western blot of FE65 immunoreactivity in human brain homogenates ( left , Non Abs. column). Absorption of the antibody (Abs. column) strongly diminished immunoreactivity. Molecular size markers are shown on the left (kd). On human brain paraffin-embedded sections, absorption of the antibody leads to similar results [compare “Non absorbed” ( middle ) and “Absorbed” ( right )]. B: FE65 immunoreactivity was observed in AD and control cases in all types of neurons such as pyramidal cells of the hippocampus as illustrated on this photograph. Neuronal nuclei were devoid of immunostained material whereas a dense cytoplasmic FE65 immunoreactivity was noted. Scale bar, 50 μm. C: Proportion of FE65 immunoreactivity (mean ± SEM) in allo- ( left ) and isocortical ( right ) samples in control (C), AD with Braak stage IV (AD IV), and AD with Braak stage VI (AD VI) cases. FE65 immunoreactivity in the hippocampus (CA4) was higher in AD cases with severe neuropathological lesions as compared to patients with less advanced pathology and nondemented/age-matched cases. No differences were observed in the isocortex between AD and control cases.

    Article Snippet: An automated immunostaining station (Nexes station; Ventana Medical Systems, Illkirch, France) was used to obtain single immunolabeling of tau protein.

    Techniques: Immunostaining, Western Blot