new england biolabs exonuclease i  (New England Biolabs)


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    Name:
    Exonuclease I E coli
    Description:
    Exonuclease I E coli 15 000 units
    Catalog Number:
    m0293l
    Price:
    281
    Size:
    15 000 units
    Category:
    Exonucleases
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    New England Biolabs new england biolabs exonuclease i
    Exonuclease I E coli
    Exonuclease I E coli 15 000 units
    https://www.bioz.com/result/new england biolabs exonuclease i/product/New England Biolabs
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    new england biolabs exonuclease i - by Bioz Stars, 2020-05
    99/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: AID–RNA polymerase II transcription-dependent deamination of IgV DNA
    Article Snippet: .. The excess of unannealed ssDNA (TS and NTS) was eliminated by treatment with 10 units of exonuclease I (NEB) at 37°C for 30 min. Exo I was removed using Monarch PCR and Reaction cleanup kit (NEB) and DNA was eluted in 20 μl of 10 mM Tris (pH 8.5). .. Library construction for Illumina sequencing Following Exo I treatment to remove ssDNA, NTS and TS strands in Pol II transcription reactions were subjected to next-generation sequencing analysis using Maximum Depth Sequencing (MDS) ( ).

    Article Title: Towards the Construction of Expressed Proteomes Using a Leishmania tarentolae Based Cell-Free Expression System
    Article Snippet: .. Variable ORF-encoded fragments were freed from residual primers by treatment with 15 U/ml Exonuclease I (NEB), which was added directly to the final PCR reaction mixture, for 30 minutes at 37°C followed by nuclease inactivation at 85°C for 30 minutes. .. Synthesis of translational templates by overlap extension (OE-PCR) PCR To obtain the libraries of DNA templates two universal fragments (1, 2 or 3, 4, ) were combined with one of the 31 variable PCR fragments using OE-PCR.

    Staining:

    Article Title: Circular Single-Stranded Synthetic DNA Delivery Vectors for MicroRNA
    Article Snippet: .. In cases where the COLIGO was still contaminated by > 5% of the linear oligonucleotide after elution (as determined by gel staining), an Exonuclease I (NEB) digest was done. ..

    Incubation:

    Article Title: Homogeneous antibody-based proximity extension assays provide sensitive and specific detection of low-abundant proteins in human blood
    Article Snippet: .. After a 5-min incubation at 37°C, a 20 µl extension mix containing 66.8 mM Tris–HCl, 16.8 mM ammonium sulfate, 1 mM dithiothreitol, 33 mM magnesium chloride, 62.5 U/ml T4 DNA Polymerase [or 62.5 U/ml Klenow fragment exo(−), 125 U/ml Klenow fragment, 125 U/ml DNA Polymerase I (Fermentas), 250 U/ml Exonuclease I (New England Biolabs)] was added. ..

    Chloramphenicol Acetyltransferase Assay:

    Article Title: Defining CRISPR-Cas9 genome-wide nuclease activities with CIRCLE-seq
    Article Snippet: .. M0293L) Lambda Exonuclease (New England BioLabs, cat.no. .. M0262L) Plasmid-Safe ATP-dependent DNase (Epicentre, cat.no.

    Avidin-Biotin Assay:

    Article Title: The structure of ends determines the pathway choice and Mre11 nuclease dependency of DNA double-strand break repair
    Article Snippet: .. To detect the presence of 3′ biotin on 3′ ss-overhangs or resection intermediates, the DNA was pre-incubated with ELB buffer or avidin on ice for 5 min, and then treated with Escherichia coli ExoI (NEB, MA) at 22ºC for 60 min. To analyze the intermediates of the 5′ biotin-avidin DNA, DNA was treated with E. coli ExoI (0.2 u/μl, NEB, MA) or RecJ (0.3 u/μl; NEB, MA) at 22°C for 60 min. To detect the presence of 5′ biotin, DNA was pre-incubated with ELB buffer or avidin on ice for 5 min, and then treated with T7 Exo (0.6 unit/μl; NEB, MA) at 22°C for 60 min. .. Reactions were analyzed by 1% TAE-agarose gel electrophoresis and the gels were first stained with SYBR Gold (Invitrogen, CA, USA) and then dried for exposure to film.

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  • 99
    New England Biolabs exonuclease i
    Circularization of DNA templates (COLIGOs) for Rolling Circle Transcription. A . Synthetic 5′ phosphorylated linear DNA sequences were circularized using the thermostable TS2126 RNA ligase. B . Denaturing polyacrylamide gel electrophoresis (DPAGE) at four stages during miR-19am DNA circle synthesis. Lane 1, crude DNA IDT Ultramer synthesis of COLIGO 19am. Lane 2, after preparative DPAGE. Lane 3, crude circularization product. Lane 4, DNA circle template following <t>Exonuclease</t> I clean-up. Visualization using Stains-All. C . Verification of circular topology. Nicking of circular templates by S1 nuclease leads first to linear forms, which are then further digested to successively smaller linear forms.
    Exonuclease I, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 512 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/exonuclease i/product/New England Biolabs
    Average 99 stars, based on 512 article reviews
    Price from $9.99 to $1999.99
    exonuclease i - by Bioz Stars, 2020-05
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    88
    New England Biolabs exo iii buffer
    Schematic of RCA of circularized padlock probes . (A) S. brasiliensis (Sbra-RCA) padlock probe design. (B) CAL is amplified by PCR with primers CAL -Fw and CAL -Rv; PCR products are submitted to ligation. Circularization of padlock probes occurs only if both probe arms hybridize correctly to the target sequence. (C) Upon specific hybridization, the phosphorylated 5′ end and the free hydroxyl at the 3′ end of the probe are joined by Pfu DNA ligase. After ligation, non-circularized probes and single-stranded primers are removed with <t>Exo</t> I and Exo <t>III</t> (optional step). (D) Circularized padlock probes serve as DNA template and signal amplification occurs via RCA, using Bst DNA polymerase and the primers RCA1 and RCA2. DNA synthesis occurs continuously for 1 h under isothermal amplification (65°C). DNA products are detected by gel electrophoresis or directly with SYBR Green I.
    Exo Iii Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    N/A
    Thermolabile Exonuclease I catalyzes the removal of nucleotides from single stranded DNA ssDNA in the 3 to 5 direction at 37°C and can be easily heat inactivated at 80°C for
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    Circularization of DNA templates (COLIGOs) for Rolling Circle Transcription. A . Synthetic 5′ phosphorylated linear DNA sequences were circularized using the thermostable TS2126 RNA ligase. B . Denaturing polyacrylamide gel electrophoresis (DPAGE) at four stages during miR-19am DNA circle synthesis. Lane 1, crude DNA IDT Ultramer synthesis of COLIGO 19am. Lane 2, after preparative DPAGE. Lane 3, crude circularization product. Lane 4, DNA circle template following Exonuclease I clean-up. Visualization using Stains-All. C . Verification of circular topology. Nicking of circular templates by S1 nuclease leads first to linear forms, which are then further digested to successively smaller linear forms.

    Journal: PLoS ONE

    Article Title: Circular Single-Stranded Synthetic DNA Delivery Vectors for MicroRNA

    doi: 10.1371/journal.pone.0016925

    Figure Lengend Snippet: Circularization of DNA templates (COLIGOs) for Rolling Circle Transcription. A . Synthetic 5′ phosphorylated linear DNA sequences were circularized using the thermostable TS2126 RNA ligase. B . Denaturing polyacrylamide gel electrophoresis (DPAGE) at four stages during miR-19am DNA circle synthesis. Lane 1, crude DNA IDT Ultramer synthesis of COLIGO 19am. Lane 2, after preparative DPAGE. Lane 3, crude circularization product. Lane 4, DNA circle template following Exonuclease I clean-up. Visualization using Stains-All. C . Verification of circular topology. Nicking of circular templates by S1 nuclease leads first to linear forms, which are then further digested to successively smaller linear forms.

    Article Snippet: In cases where the COLIGO was still contaminated by > 5% of the linear oligonucleotide after elution (as determined by gel staining), an Exonuclease I (NEB) digest was done.

    Techniques: Polyacrylamide Gel Electrophoresis

    Exonuclease activity and hybridization length affects assay sensitivity. (A) VEGF assays were designed with different lengths of the hybridization site and compared with respect to sensitivity. A 9-nt hybridization site was found to give the best signal-to-noise levels and was selected for further studies. ( B ) Different DNA polymerases were tested with regards to their ability to generate good sensitivity in an IL-8-specific assay. T4 DNA polymerase I, DNA polymerase I and Klenow fragment exo + all possess a 3′→5′ exonuclease activity and performed well in the IL-8 detection. Klenow fragment exo − , on the other hand, generated a background signal that was almost at the level of the antigen-induced signal. When exogenous Exonuclease I was added to the reaction, the signal-to-noise level was restored.

    Journal: Nucleic Acids Research

    Article Title: Homogeneous antibody-based proximity extension assays provide sensitive and specific detection of low-abundant proteins in human blood

    doi: 10.1093/nar/gkr424

    Figure Lengend Snippet: Exonuclease activity and hybridization length affects assay sensitivity. (A) VEGF assays were designed with different lengths of the hybridization site and compared with respect to sensitivity. A 9-nt hybridization site was found to give the best signal-to-noise levels and was selected for further studies. ( B ) Different DNA polymerases were tested with regards to their ability to generate good sensitivity in an IL-8-specific assay. T4 DNA polymerase I, DNA polymerase I and Klenow fragment exo + all possess a 3′→5′ exonuclease activity and performed well in the IL-8 detection. Klenow fragment exo − , on the other hand, generated a background signal that was almost at the level of the antigen-induced signal. When exogenous Exonuclease I was added to the reaction, the signal-to-noise level was restored.

    Article Snippet: After a 5-min incubation at 37°C, a 20 µl extension mix containing 66.8 mM Tris–HCl, 16.8 mM ammonium sulfate, 1 mM dithiothreitol, 33 mM magnesium chloride, 62.5 U/ml T4 DNA Polymerase [or 62.5 U/ml Klenow fragment exo(−), 125 U/ml Klenow fragment, 125 U/ml DNA Polymerase I (Fermentas), 250 U/ml Exonuclease I (New England Biolabs)] was added.

    Techniques: Activity Assay, Hybridization, Generated

    Schematic diagram of procedures of liquid hybridization and solid phase detection (LHSPD). ( 1 ) Liquid hybridization: The small RNA samples, hybridization buffer, and probe are mixed in a tube to make the probe hybridize with the specific RNA sequences and the non-hybridized sequences are digested by exonuclease I; ( 2 ) Gel electrophoresis: the products of the hybridization are separated by electrophoresis; ( 3 ) Transfer membrane; ( 4 ) UV crosslinking and membrane blocking; ( 5 ) Antibody incubation: alkaline phosphatase (AP)-anti-DIG antibody or AP-streptavidin or horseradish peroxidase (HRP)-streptavidin targeted the RNA-bound DIG-labeled probes or biotin-labeled probes respectively; ( 6 ) Hybridization signal detection: CDP-Star/luminol is used to detect the combination of antibody and target RNA.

    Journal: International Journal of Molecular Sciences

    Article Title: Liquid Hybridization and Solid Phase Detection: A Highly Sensitive and Accurate Strategy for MicroRNA Detection in Plants and Animals

    doi: 10.3390/ijms17091457

    Figure Lengend Snippet: Schematic diagram of procedures of liquid hybridization and solid phase detection (LHSPD). ( 1 ) Liquid hybridization: The small RNA samples, hybridization buffer, and probe are mixed in a tube to make the probe hybridize with the specific RNA sequences and the non-hybridized sequences are digested by exonuclease I; ( 2 ) Gel electrophoresis: the products of the hybridization are separated by electrophoresis; ( 3 ) Transfer membrane; ( 4 ) UV crosslinking and membrane blocking; ( 5 ) Antibody incubation: alkaline phosphatase (AP)-anti-DIG antibody or AP-streptavidin or horseradish peroxidase (HRP)-streptavidin targeted the RNA-bound DIG-labeled probes or biotin-labeled probes respectively; ( 6 ) Hybridization signal detection: CDP-Star/luminol is used to detect the combination of antibody and target RNA.

    Article Snippet: After that, non-hybridized single-stranded DNA, including the probe, was digested with 1 U exonuclease I (New England BioLabs, Inc., M0293, Beijing, China) in the same tube according to the instruction protocol for 30 min at 37 °C.

    Techniques: Hybridization, Nucleic Acid Electrophoresis, Electrophoresis, Blocking Assay, Incubation, Labeling

    Specificity of LHSPD at different temperatures. ( A – D ) Hybridizations of 0.1 pmol ( DIG ) -miD156rk with 1 pmol miD156s at different temperatures by LHSPD; ( E – H ) Hybridizations of 0.1 pmol ( Biotin ) -miD156rk with 1 pmol miD156s at different temperatures by LHSPD; ( I – L ) Hybridizations of 0.1 pmol ( Biotin ) -miD156rk with 1 pmol miD156s at different temperatures by traditional Northern hybridization. miD156 marked “+”, miD156 with one-base mismatch marked “−1”, miD156 with three-base mismatch marked “−3” and miD156 with five-base mismatch marked “−5”; ( M ) Hybridization performed in 0.25×, 0.5× and 1× Exonuclease I reaction buffer (New England Biolabs, Inc., Beijing, China) with 0.1 pmol ( Biotin ) -miD156rk ; ( N ) Hybridization performed in 0.25×, 0.5× and 1× PNE buffer with 0.1 pmol ( Biotin ) -miD156rk ; 20f represents 20 fmol of miD156 ; 10f represents 10 fmol of miD156 ; 5f represents 5 fmol of miD156 ; P represents control containing 1 pmol of ( Biotin ) -miD156rk .

    Journal: International Journal of Molecular Sciences

    Article Title: Liquid Hybridization and Solid Phase Detection: A Highly Sensitive and Accurate Strategy for MicroRNA Detection in Plants and Animals

    doi: 10.3390/ijms17091457

    Figure Lengend Snippet: Specificity of LHSPD at different temperatures. ( A – D ) Hybridizations of 0.1 pmol ( DIG ) -miD156rk with 1 pmol miD156s at different temperatures by LHSPD; ( E – H ) Hybridizations of 0.1 pmol ( Biotin ) -miD156rk with 1 pmol miD156s at different temperatures by LHSPD; ( I – L ) Hybridizations of 0.1 pmol ( Biotin ) -miD156rk with 1 pmol miD156s at different temperatures by traditional Northern hybridization. miD156 marked “+”, miD156 with one-base mismatch marked “−1”, miD156 with three-base mismatch marked “−3” and miD156 with five-base mismatch marked “−5”; ( M ) Hybridization performed in 0.25×, 0.5× and 1× Exonuclease I reaction buffer (New England Biolabs, Inc., Beijing, China) with 0.1 pmol ( Biotin ) -miD156rk ; ( N ) Hybridization performed in 0.25×, 0.5× and 1× PNE buffer with 0.1 pmol ( Biotin ) -miD156rk ; 20f represents 20 fmol of miD156 ; 10f represents 10 fmol of miD156 ; 5f represents 5 fmol of miD156 ; P represents control containing 1 pmol of ( Biotin ) -miD156rk .

    Article Snippet: After that, non-hybridized single-stranded DNA, including the probe, was digested with 1 U exonuclease I (New England BioLabs, Inc., M0293, Beijing, China) in the same tube according to the instruction protocol for 30 min at 37 °C.

    Techniques: Northern Blot, Hybridization

    Schematic of RCA of circularized padlock probes . (A) S. brasiliensis (Sbra-RCA) padlock probe design. (B) CAL is amplified by PCR with primers CAL -Fw and CAL -Rv; PCR products are submitted to ligation. Circularization of padlock probes occurs only if both probe arms hybridize correctly to the target sequence. (C) Upon specific hybridization, the phosphorylated 5′ end and the free hydroxyl at the 3′ end of the probe are joined by Pfu DNA ligase. After ligation, non-circularized probes and single-stranded primers are removed with Exo I and Exo III (optional step). (D) Circularized padlock probes serve as DNA template and signal amplification occurs via RCA, using Bst DNA polymerase and the primers RCA1 and RCA2. DNA synthesis occurs continuously for 1 h under isothermal amplification (65°C). DNA products are detected by gel electrophoresis or directly with SYBR Green I.

    Journal: Frontiers in Microbiology

    Article Title: Rapid Identification of Emerging Human-Pathogenic Sporothrix Species with Rolling Circle Amplification

    doi: 10.3389/fmicb.2015.01385

    Figure Lengend Snippet: Schematic of RCA of circularized padlock probes . (A) S. brasiliensis (Sbra-RCA) padlock probe design. (B) CAL is amplified by PCR with primers CAL -Fw and CAL -Rv; PCR products are submitted to ligation. Circularization of padlock probes occurs only if both probe arms hybridize correctly to the target sequence. (C) Upon specific hybridization, the phosphorylated 5′ end and the free hydroxyl at the 3′ end of the probe are joined by Pfu DNA ligase. After ligation, non-circularized probes and single-stranded primers are removed with Exo I and Exo III (optional step). (D) Circularized padlock probes serve as DNA template and signal amplification occurs via RCA, using Bst DNA polymerase and the primers RCA1 and RCA2. DNA synthesis occurs continuously for 1 h under isothermal amplification (65°C). DNA products are detected by gel electrophoresis or directly with SYBR Green I.

    Article Snippet: The exonucleolysis mix consisted of 0.5 μL Exo I (New England BioLabs, Ipswich, MA, USA), 1 μL 10 × Exo I Buffer (New England BioLabs), 0.2 μL Exo III (New England BioLabs), 1 μL 10 × Exo III Buffer (New England BioLabs), and 7.3 μL ultra-pure water.

    Techniques: Amplification, Polymerase Chain Reaction, Ligation, Sequencing, Hybridization, DNA Synthesis, Nucleic Acid Electrophoresis, SYBR Green Assay