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PrimerDesign Inc nested pcr strategy
Nested Pcr Strategy, supplied by PrimerDesign Inc, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/nested pcr strategy/product/PrimerDesign Inc
Average 88 stars, based on 1 article reviews
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nested pcr strategy - by Bioz Stars, 2020-07
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Nested PCR:

Article Title: Tracking the Evolution of the SARS Coronavirus Using High-Throughput, High-Density Resequencing Arrays
Article Snippet: .. Tissue samples were amplified using a nested-PCR strategy (see Primer Design below). ..

Polymerase Chain Reaction:

Article Title: Domain selection combined with improved cloning strategy for high throughput expression of higher eukaryotic proteins
Article Snippet: .. Since the PCS was included in the primer synthesis in our strategy and the long forward primer would be costly and could increase the chance of errors, we designed a PCR strategy using two forward primers and two reverse primers (see Methods: Primer design and the PCR protocol for HTP cloning). .. This strategy has two advantages: only short primers are required, and primer F2, R2 could be synthesized in bulk.

Amplification:

Article Title: Tracking the Evolution of the SARS Coronavirus Using High-Throughput, High-Density Resequencing Arrays
Article Snippet: .. Tissue samples were amplified using a nested-PCR strategy (see Primer Design below). ..

Clone Assay:

Article Title: Domain selection combined with improved cloning strategy for high throughput expression of higher eukaryotic proteins
Article Snippet: .. Since the PCS was included in the primer synthesis in our strategy and the long forward primer would be costly and could increase the chance of errors, we designed a PCR strategy using two forward primers and two reverse primers (see Methods: Primer design and the PCR protocol for HTP cloning). .. This strategy has two advantages: only short primers are required, and primer F2, R2 could be synthesized in bulk.

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    PrimerDesign Inc consensus degenerate hybrid oligonucleotide primer codehop pcr strategy
    The <t>CODEHOP</t> <t>PCR</t> strategy. CODEHOP PCR primers target conserved motifs of 3–4 amino acids using a short 3′ degenerate core containing all codon possibilities for the motif. Annealing of the degenerate core of the primer to the starting template is stabilized by the 5′ consensus clamp. During subsequent rounds of amplification, annealing of primer to PCR product is driven by the identical match with the 5′ consensus clamp between the incorporated primer and primers remaining in the pool. This strategy permits amplification of distantly related sequences with limited homology to known family members.
    Consensus Degenerate Hybrid Oligonucleotide Primer Codehop Pcr Strategy, supplied by PrimerDesign Inc, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/consensus degenerate hybrid oligonucleotide primer codehop pcr strategy/product/PrimerDesign Inc
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    consensus degenerate hybrid oligonucleotide primer codehop pcr strategy - by Bioz Stars, 2020-07
    85/100 stars
      Buy from Supplier

    84
    PrimerDesign Inc pcr primer design strategy
    The <t>CODEHOP</t> <t>PCR</t> strategy. CODEHOP PCR primers target conserved motifs of 3–4 amino acids using a short 3′ degenerate core containing all codon possibilities for the motif. Annealing of the degenerate core of the primer to the starting template is stabilized by the 5′ consensus clamp. During subsequent rounds of amplification, annealing of primer to PCR product is driven by the identical match with the 5′ consensus clamp between the incorporated primer and primers remaining in the pool. This strategy permits amplification of distantly related sequences with limited homology to known family members.
    Pcr Primer Design Strategy, supplied by PrimerDesign Inc, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr primer design strategy/product/PrimerDesign Inc
    Average 84 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pcr primer design strategy - by Bioz Stars, 2020-07
    84/100 stars
      Buy from Supplier

    88
    PrimerDesign Inc nested pcr strategy
    The <t>CODEHOP</t> <t>PCR</t> strategy. CODEHOP PCR primers target conserved motifs of 3–4 amino acids using a short 3′ degenerate core containing all codon possibilities for the motif. Annealing of the degenerate core of the primer to the starting template is stabilized by the 5′ consensus clamp. During subsequent rounds of amplification, annealing of primer to PCR product is driven by the identical match with the 5′ consensus clamp between the incorporated primer and primers remaining in the pool. This strategy permits amplification of distantly related sequences with limited homology to known family members.
    Nested Pcr Strategy, supplied by PrimerDesign Inc, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nested pcr strategy/product/PrimerDesign Inc
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    nested pcr strategy - by Bioz Stars, 2020-07
    88/100 stars
      Buy from Supplier

    85
    PrimerDesign Inc mirna specific qpcr primers
    The <t>CODEHOP</t> <t>PCR</t> strategy. CODEHOP PCR primers target conserved motifs of 3–4 amino acids using a short 3′ degenerate core containing all codon possibilities for the motif. Annealing of the degenerate core of the primer to the starting template is stabilized by the 5′ consensus clamp. During subsequent rounds of amplification, annealing of primer to PCR product is driven by the identical match with the 5′ consensus clamp between the incorporated primer and primers remaining in the pool. This strategy permits amplification of distantly related sequences with limited homology to known family members.
    Mirna Specific Qpcr Primers, supplied by PrimerDesign Inc, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mirna specific qpcr primers/product/PrimerDesign Inc
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mirna specific qpcr primers - by Bioz Stars, 2020-07
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    The CODEHOP PCR strategy. CODEHOP PCR primers target conserved motifs of 3–4 amino acids using a short 3′ degenerate core containing all codon possibilities for the motif. Annealing of the degenerate core of the primer to the starting template is stabilized by the 5′ consensus clamp. During subsequent rounds of amplification, annealing of primer to PCR product is driven by the identical match with the 5′ consensus clamp between the incorporated primer and primers remaining in the pool. This strategy permits amplification of distantly related sequences with limited homology to known family members.

    Journal: Nucleic Acids Research

    Article Title: CODEHOP (COnsensus-DEgenerate Hybrid Oligonucleotide Primer) PCR primer design

    doi:

    Figure Lengend Snippet: The CODEHOP PCR strategy. CODEHOP PCR primers target conserved motifs of 3–4 amino acids using a short 3′ degenerate core containing all codon possibilities for the motif. Annealing of the degenerate core of the primer to the starting template is stabilized by the 5′ consensus clamp. During subsequent rounds of amplification, annealing of primer to PCR product is driven by the identical match with the 5′ consensus clamp between the incorporated primer and primers remaining in the pool. This strategy permits amplification of distantly related sequences with limited homology to known family members.

    Article Snippet: We have developed the COnsensus-DEgenerate Hybrid Oligonucleotide Primer (CODEHOP) PCR strategy for the identification of new members of protein families, which overcomes problems inherent in both degenerate and consensus methods for primer design ( ).

    Techniques: Polymerase Chain Reaction, Amplification