Journal:
Article Title: Comparative Sequence Analysis of the tuf and recA Genes and Restriction Fragment Length Polymorphism of the Internal Transcribed Spacer Region Sequences Supply Additional Tools for Discriminating Bifidobacterium lactis from Bifidobacterium animalis
doi: 10.1128/AEM.69.12.7517-7522.2003
Figure Lengend Snippet: Restriction patterns of PCR-amplified fragments of 16S-23S rDNA digested with Sau3AI. Lane 1, B. animalis ATCC 25527; lane 2, B. animalis ATCC 27672; lane 3, B. lactis DSM 10140; lane 4, B. animalis ATCC 27674; lane 5, B. animalis ATCC 27673; lane 6, B. animalis ATCC 27536; lane 7, B. lactis NCC 363; lane 8, B. lactis NCC 311; lane 9, B. lactis NCC 402; lane 10, B. lactis NCC 311; lane 11, B. lactis NCC 383; lane 12, B. lactis NCC 239; lanes M, 50-bp DNA ladder (Gibco BRL).
Article Snippet: Recently, a reorganization of the B. animalis species with the rejection of few strains (ATCC 27673, ATCC 27674, and ATCC 27536) from this species and a reclassification of them as B. lactis have been proposed ( 19 ).
Techniques: Amplification