Structured Review

Thermo Fisher ned
Standard curves showing the initial DNA quantity versus threshold cycle (Ct). The standard curves were generated by three different real-time <t>PCR</t> assay conditions using serial dilutions of DNA from t(14;18)-positive cell lines: blue , mbr or mcr alone; red , co-amplification of mbr or mcr with cyclophilin ; green , co-amplification of mbr or mcr with cyclophilin and using a <t>NED-labeled</t> JH primer.
Ned, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 67 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Price from $9.99 to $1999.99
ned - by Bioz Stars, 2020-08
99/100 stars

Images

1) Product Images from "Quantification of bcl-2/JH Fusion Sequences and a Control Gene by Multiplex Real-Time PCR Coupled with Automated Amplicon Sizing by Capillary Electrophoresis"

Article Title: Quantification of bcl-2/JH Fusion Sequences and a Control Gene by Multiplex Real-Time PCR Coupled with Automated Amplicon Sizing by Capillary Electrophoresis

Journal: The Journal of molecular diagnostics : JMD

doi:

Standard curves showing the initial DNA quantity versus threshold cycle (Ct). The standard curves were generated by three different real-time PCR assay conditions using serial dilutions of DNA from t(14;18)-positive cell lines: blue , mbr or mcr alone; red , co-amplification of mbr or mcr with cyclophilin ; green , co-amplification of mbr or mcr with cyclophilin and using a NED-labeled JH primer.
Figure Legend Snippet: Standard curves showing the initial DNA quantity versus threshold cycle (Ct). The standard curves were generated by three different real-time PCR assay conditions using serial dilutions of DNA from t(14;18)-positive cell lines: blue , mbr or mcr alone; red , co-amplification of mbr or mcr with cyclophilin ; green , co-amplification of mbr or mcr with cyclophilin and using a NED-labeled JH primer.

Techniques Used: Generated, Real-time Polymerase Chain Reaction, Amplification, Labeling

2) Product Images from "A post-labeling method for multiplexed and multicolored genotyping analysis of SSR, indel and SNP markers in single tube with bar-coded split tag (BStag)"

Article Title: A post-labeling method for multiplexed and multicolored genotyping analysis of SSR, indel and SNP markers in single tube with bar-coded split tag (BStag)

Journal: BMC Research Notes

doi: 10.1186/1756-0500-4-161

An application of post-PCR labeling with BStag for SNP markers . Two SSR markers listed in Table 3 demonstrated successful labeling of each allele with different colors using BStag. A marker LLL0729 demonstrated segregation of C/G alleles for two different citrus hybrids (C/G: a hybrid of C. kunip × C. kinokuni ; C/C: a hybrid of 'Lee' × C. kinokuni ) labeled with NED (allele C) and PET (allele G). Another marker MAPGR75R also demonstrated segregation of A/T alleles for three citrus accessions: A/A for 'Hayasaki' ( C. grandis 'Mato' × C. grandis 'Hirado'), T/T for a hybrid of C. kunip × C. kinokuni , and A/T for Satsuma mandarin.
Figure Legend Snippet: An application of post-PCR labeling with BStag for SNP markers . Two SSR markers listed in Table 3 demonstrated successful labeling of each allele with different colors using BStag. A marker LLL0729 demonstrated segregation of C/G alleles for two different citrus hybrids (C/G: a hybrid of C. kunip × C. kinokuni ; C/C: a hybrid of 'Lee' × C. kinokuni ) labeled with NED (allele C) and PET (allele G). Another marker MAPGR75R also demonstrated segregation of A/T alleles for three citrus accessions: A/A for 'Hayasaki' ( C. grandis 'Mato' × C. grandis 'Hirado'), T/T for a hybrid of C. kunip × C. kinokuni , and A/T for Satsuma mandarin.

Techniques Used: Polymerase Chain Reaction, Labeling, Marker, Positron Emission Tomography

Electropherogram of four SSR markers obtained by multiplexed post PCR labeling in a single tube . Genotyping of four SSR markers listed in Table 2 successfully gave individual peaks labeled with different fluorescent dyes in a single tube. 1) SSR08A04/F9GCC + VIC (green). 2) SSR08A09/F9GTC + PET (red). 3) SSR08B15/F9GAC + NED (black). 4) SSR08B25/F9TAC + 6-FAM (blue). Numbers under each peak represent estimated fragment size. These electropherograms were obtained for Binkitsu ( C. platymamma hort. ex Tanaka).
Figure Legend Snippet: Electropherogram of four SSR markers obtained by multiplexed post PCR labeling in a single tube . Genotyping of four SSR markers listed in Table 2 successfully gave individual peaks labeled with different fluorescent dyes in a single tube. 1) SSR08A04/F9GCC + VIC (green). 2) SSR08A09/F9GTC + PET (red). 3) SSR08B15/F9GAC + NED (black). 4) SSR08B25/F9TAC + 6-FAM (blue). Numbers under each peak represent estimated fragment size. These electropherograms were obtained for Binkitsu ( C. platymamma hort. ex Tanaka).

Techniques Used: Polymerase Chain Reaction, Labeling, Positron Emission Tomography

3) Product Images from "Multiplex TaqMan qPCR assay for specific identification of encapsulated Trichinella species prevalent in North America"

Article Title: Multiplex TaqMan qPCR assay for specific identification of encapsulated Trichinella species prevalent in North America

Journal: Memórias do Instituto Oswaldo Cruz

doi: 10.1590/0074-02760180305

amplification plots of Trichinella quantitative real-time polymerase chain reaction (qPCR) multiplex assay using T. nativa , T. spiralis , T. murrelli and Trichinella T6 specific probes. Threshold baselines are colored in blue - VIC - T. native (panel A), yellow - FAM - T. murrelli (panel B), violet - CY5 - T. spiralis (panel C) and green - NED - Trichinella T6 (panel D).
Figure Legend Snippet: amplification plots of Trichinella quantitative real-time polymerase chain reaction (qPCR) multiplex assay using T. nativa , T. spiralis , T. murrelli and Trichinella T6 specific probes. Threshold baselines are colored in blue - VIC - T. native (panel A), yellow - FAM - T. murrelli (panel B), violet - CY5 - T. spiralis (panel C) and green - NED - Trichinella T6 (panel D).

Techniques Used: Amplification, Real-time Polymerase Chain Reaction, Multiplex Assay

4) Product Images from "Selection for Phase Variation of LOS Biosynthetic Genes Frequently Occurs in Progression of Non-Typeable Haemophilus influenzae Infection from the Nasopharynx to the Middle Ear of Human Patients"

Article Title: Selection for Phase Variation of LOS Biosynthetic Genes Frequently Occurs in Progression of Non-Typeable Haemophilus influenzae Infection from the Nasopharynx to the Middle Ear of Human Patients

Journal: PLoS ONE

doi: 10.1371/journal.pone.0090505

LOS biosynthesis pathway in Haemophilus influenzae , and examples of multiplex PCR and fragment analysis traces. ( A ) an illustration of the roles of the proteins encoded by the seven phasevariable genes studied in this work: Lic1 = phosphocholine transferase; Lic2A = galactosyltransferase; Lic3A and Lic3B = analogous sialyltransferases; Lex2 = glucosyltransferase; OafA = O-acetylase; LgtC = galactosyltransferase. The LOS contains 2-keto-3-deoxyoctulosonic acid (KDO); phosphoethanolamine (PEtn); pyrophosphoethanolamine (PPEtn); heptose (Hep); galactose (Gal); glucose (Glc); phosphocholine (PCho); and O-acetyl group (OAc); ( B ) an example of a multiplex PCR reaction utilized in this study using three gene specific primer pairs, with each pair containing a forward primer with a specific fluorescent label: FAM (6-carboxyflourescein), VIC, or NED; and ( C ) an example of a typical fragment analysis trace generated by a fluorescent PCR reaction separated using the Genescan system.
Figure Legend Snippet: LOS biosynthesis pathway in Haemophilus influenzae , and examples of multiplex PCR and fragment analysis traces. ( A ) an illustration of the roles of the proteins encoded by the seven phasevariable genes studied in this work: Lic1 = phosphocholine transferase; Lic2A = galactosyltransferase; Lic3A and Lic3B = analogous sialyltransferases; Lex2 = glucosyltransferase; OafA = O-acetylase; LgtC = galactosyltransferase. The LOS contains 2-keto-3-deoxyoctulosonic acid (KDO); phosphoethanolamine (PEtn); pyrophosphoethanolamine (PPEtn); heptose (Hep); galactose (Gal); glucose (Glc); phosphocholine (PCho); and O-acetyl group (OAc); ( B ) an example of a multiplex PCR reaction utilized in this study using three gene specific primer pairs, with each pair containing a forward primer with a specific fluorescent label: FAM (6-carboxyflourescein), VIC, or NED; and ( C ) an example of a typical fragment analysis trace generated by a fluorescent PCR reaction separated using the Genescan system.

Techniques Used: Multiplex Assay, Polymerase Chain Reaction, Gas Chromatography, Generated

5) Product Images from "Multiplex TaqMan qPCR assay for specific identification of encapsulated Trichinella species prevalent in North America"

Article Title: Multiplex TaqMan qPCR assay for specific identification of encapsulated Trichinella species prevalent in North America

Journal: Memórias do Instituto Oswaldo Cruz

doi: 10.1590/0074-02760180305

amplification plots of Trichinella quantitative real-time polymerase chain reaction (qPCR) multiplex assay using T. nativa , T. spiralis , T. murrelli and Trichinella T6 specific probes. Threshold baselines are colored in blue - VIC - T. native (panel A), yellow - FAM - T. murrelli (panel B), violet - CY5 - T. spiralis (panel C) and green - NED - Trichinella T6 (panel D).
Figure Legend Snippet: amplification plots of Trichinella quantitative real-time polymerase chain reaction (qPCR) multiplex assay using T. nativa , T. spiralis , T. murrelli and Trichinella T6 specific probes. Threshold baselines are colored in blue - VIC - T. native (panel A), yellow - FAM - T. murrelli (panel B), violet - CY5 - T. spiralis (panel C) and green - NED - Trichinella T6 (panel D).

Techniques Used: Amplification, Real-time Polymerase Chain Reaction, Multiplex Assay

Related Articles

Polymerase Chain Reaction:

Article Title: Markers for Mapping by Admixture Linkage Disequilibrium in African American and Hispanic Populations
Article Snippet: .. Fluorescently labeled PCR products (FAM, HEX, TET, and NED) were separated on Applied Biosystems 373 and 377 sequencers. .. Gels were analyzed with Genescan collection and analysis software, and genotypes were called using Genotyper software (Applied Biosystems).

Article Title: Genetic diversity and distribution of Senegalia senegal (L.) Britton under climate change scenarios in West Africa
Article Snippet: .. PCR products were amplified using 6-FAM-, VIC-, NED-, and PET-labelled primers (Applied Biosystems, Foster City, California, USA). .. The touchdown cycling program was used on a Mastercycler 2050 model thermal cycler (Thermo Fisher Scientific, Waltham, Massachusetts, USA).

Article Title: Sperm competitiveness in frogs: slow and steady wins the race
Article Snippet: .. These were multiplexed into one polymerase chain reaction (PCR) containing 1× PCR buffer (10 mM Tris–HCl pH 8.3, 50 mM KCl) (Invitrogen), 3 mM MgCl2 (Invitrogen), 200 µM of each dNTP (Invitrogen), 250 nM of each forward primer, Cg2Ca24 labelled with NED (Applied Biosystems), Cg3Ca8 labelled with VIC (Applied Biosystems), Cg1Ca9 labelled with 6-FAM (Geneworks) (each labelled primer was diluted with unlabelled primer 1:10, except Cg1Ca9 which was diluted 1:1), 250 nM of each reverse primer, 0.5 U of Platinum Taq polymerase (Invitrogen) and 1–10 ng DNA. .. PCR amplification was performed with the following cycling conditions: 94°C for 3 min, then 30 cycles of 94°C for 30 s, 57°C for 30 s and 72°C for 1 min and finally 72°C for 30 min.

Amplification:

Article Title: Two closely related species differ in their regional genetic differentiation despite admixing
Article Snippet: .. DNA from each sample was amplified with the common tag containing one of four fluorescent dyes, 6-FAM, PET, VIC or NED (Applied Biosystems). .. PCRs were carried out as follows: preliminary denaturation at 95 °C for 5 min, 35 cycles at 95 °C for 1 min, annealing temperature 53.6–60 °C for 1 min, 72 °C for 1 min and a final extension step at 72 °C for 30 min, using a Techne TC-5000 thermocycler (Bibby Scientific).

Article Title: Diversity of Mycoplasma hominis clinical isolates from Bordeaux, France, as assessed by multiple-locus variable-number tandem repeat analysis
Article Snippet: .. The forward primers were fluorescently labelled at the 5’ end using 4,4,7,2’,4’,5’,7’-hexachloro-6-carboxy-fluorescein (HEX), 6-carboxyfluorescein (FAM; Eurogentec, Angers, France) or NED (2’-chloro-5’-fluoro-7’,8’-fused phenyl-1,4-dichloro-6-carboxyfluorescein; Applied Biosystems, Life Technologies, Carlsbad, CA, USA), depending on the locus to be amplified (Additional file : Table S2). .. Prior to GeneScan analysis, 0.3 μl of GeneScan ROX 500 size standard (Applied Biosystems) was added to 1 μl of each PCR product.

Article Title: Genetic diversity and distribution of Senegalia senegal (L.) Britton under climate change scenarios in West Africa
Article Snippet: .. PCR products were amplified using 6-FAM-, VIC-, NED-, and PET-labelled primers (Applied Biosystems, Foster City, California, USA). .. The touchdown cycling program was used on a Mastercycler 2050 model thermal cycler (Thermo Fisher Scientific, Waltham, Massachusetts, USA).

Labeling:

Article Title: Characterization of microsatellite markers for pinedrops, Pterospora andromedea (Ericaceae), from Illumina MiSeq sequencing 1
Article Snippet: .. From the 33 loci tested, 19 were chosen for screening using fluorescently labeled (6FAM, VIC, PET, NED; Applied Biosystems, Foster City, California, USA) forward primers as described by . .. Fragment analysis was conducted using the GeneScan 500 LIZ Size Standard (Applied Biosystems) on an ABI 3730 DNA Analyzer (Applied Biosystems) by the Biotechnology Resource Center (BRC) at Cornell University.

Article Title: Variation in fine‐scale genetic structure and local dispersal patterns between peripheral populations of a South American passerine bird, et al. Variation in fine‐scale genetic structure and local dispersal patterns between peripheral populations of a South American passerine bird
Article Snippet: .. The forward primer of each pair was fluorescently labeled with 6‐FAM™ , VIC® , PET® , or NED™ (Dye Set G5; Thermo Fisher Scientific). .. Primer concentrations in these mixes were adapted due to differences in amplification efficiency and dye strength (see Table ).

Article Title: Markers for Mapping by Admixture Linkage Disequilibrium in African American and Hispanic Populations
Article Snippet: .. Fluorescently labeled PCR products (FAM, HEX, TET, and NED) were separated on Applied Biosystems 373 and 377 sequencers. .. Gels were analyzed with Genescan collection and analysis software, and genotypes were called using Genotyper software (Applied Biosystems).

Positron Emission Tomography:

Article Title: Characterization of microsatellite markers for pinedrops, Pterospora andromedea (Ericaceae), from Illumina MiSeq sequencing 1
Article Snippet: .. From the 33 loci tested, 19 were chosen for screening using fluorescently labeled (6FAM, VIC, PET, NED; Applied Biosystems, Foster City, California, USA) forward primers as described by . .. Fragment analysis was conducted using the GeneScan 500 LIZ Size Standard (Applied Biosystems) on an ABI 3730 DNA Analyzer (Applied Biosystems) by the Biotechnology Resource Center (BRC) at Cornell University.

Article Title: Variation in fine‐scale genetic structure and local dispersal patterns between peripheral populations of a South American passerine bird, et al. Variation in fine‐scale genetic structure and local dispersal patterns between peripheral populations of a South American passerine bird
Article Snippet: .. The forward primer of each pair was fluorescently labeled with 6‐FAM™ , VIC® , PET® , or NED™ (Dye Set G5; Thermo Fisher Scientific). .. Primer concentrations in these mixes were adapted due to differences in amplification efficiency and dye strength (see Table ).

Article Title: Two closely related species differ in their regional genetic differentiation despite admixing
Article Snippet: .. DNA from each sample was amplified with the common tag containing one of four fluorescent dyes, 6-FAM, PET, VIC or NED (Applied Biosystems). .. PCRs were carried out as follows: preliminary denaturation at 95 °C for 5 min, 35 cycles at 95 °C for 1 min, annealing temperature 53.6–60 °C for 1 min, 72 °C for 1 min and a final extension step at 72 °C for 30 min, using a Techne TC-5000 thermocycler (Bibby Scientific).

Article Title: Genetic diversity and distribution of Senegalia senegal (L.) Britton under climate change scenarios in West Africa
Article Snippet: .. PCR products were amplified using 6-FAM-, VIC-, NED-, and PET-labelled primers (Applied Biosystems, Foster City, California, USA). .. The touchdown cycling program was used on a Mastercycler 2050 model thermal cycler (Thermo Fisher Scientific, Waltham, Massachusetts, USA).

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  • 91
    Thermo Fisher ned 19
    <t>Ned-19</t> activity on different stages of the P. falciparum asexual cycle. Synchronized ring stage parasites (initial parasitaemia 1.4%) were used to yield subcultures, numbered 1–8, which were incubated with DMSO control ( blue line ) or exposed to 100 μM Ned-19 ( red line ) at different times of the parasite asexual cycle. a Stages of the parasite asexual cycle. Black arrows indicate times at which the cultures were sampled. b Histograms showing parasitaemia and distribution of the different parasite stages for the cultures in each time-point. ER early rings, LR late rings, ET early trophozoites, LT late trophozoites, ES early schizonts, LS late schizonts. A representative sample of these forms is shown in Additional file 2 : Figure S2
    Ned 19, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ned 19/product/Thermo Fisher
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ned 19 - by Bioz Stars, 2020-08
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    84
    Thermo Fisher ned matrix standard
    <t>Ned-19</t> activity on different stages of the P. falciparum asexual cycle. Synchronized ring stage parasites (initial parasitaemia 1.4%) were used to yield subcultures, numbered 1–8, which were incubated with DMSO control ( blue line ) or exposed to 100 μM Ned-19 ( red line ) at different times of the parasite asexual cycle. a Stages of the parasite asexual cycle. Black arrows indicate times at which the cultures were sampled. b Histograms showing parasitaemia and distribution of the different parasite stages for the cultures in each time-point. ER early rings, LR late rings, ET early trophozoites, LT late trophozoites, ES early schizonts, LS late schizonts. A representative sample of these forms is shown in Additional file 2 : Figure S2
    Ned Matrix Standard, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ned matrix standard/product/Thermo Fisher
    Average 84 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ned matrix standard - by Bioz Stars, 2020-08
    84/100 stars
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    94
    Thermo Fisher ned
    <t>Ned-19</t> activity on different stages of the P. falciparum asexual cycle. Synchronized ring stage parasites (initial parasitaemia 1.4%) were used to yield subcultures, numbered 1–8, which were incubated with DMSO control ( blue line ) or exposed to 100 μM Ned-19 ( red line ) at different times of the parasite asexual cycle. a Stages of the parasite asexual cycle. Black arrows indicate times at which the cultures were sampled. b Histograms showing parasitaemia and distribution of the different parasite stages for the cultures in each time-point. ER early rings, LR late rings, ET early trophozoites, LT late trophozoites, ES early schizonts, LS late schizonts. A representative sample of these forms is shown in Additional file 2 : Figure S2
    Ned, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 67 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ned/product/Thermo Fisher
    Average 94 stars, based on 67 article reviews
    Price from $9.99 to $1999.99
    ned - by Bioz Stars, 2020-08
    94/100 stars
      Buy from Supplier

    91
    Thermo Fisher ned yellow fluorescent dyes
    <t>Ned-19</t> activity on different stages of the P. falciparum asexual cycle. Synchronized ring stage parasites (initial parasitaemia 1.4%) were used to yield subcultures, numbered 1–8, which were incubated with DMSO control ( blue line ) or exposed to 100 μM Ned-19 ( red line ) at different times of the parasite asexual cycle. a Stages of the parasite asexual cycle. Black arrows indicate times at which the cultures were sampled. b Histograms showing parasitaemia and distribution of the different parasite stages for the cultures in each time-point. ER early rings, LR late rings, ET early trophozoites, LT late trophozoites, ES early schizonts, LS late schizonts. A representative sample of these forms is shown in Additional file 2 : Figure S2
    Ned Yellow Fluorescent Dyes, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ned yellow fluorescent dyes/product/Thermo Fisher
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    ned yellow fluorescent dyes - by Bioz Stars, 2020-08
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      Buy from Supplier

    Image Search Results


    Ned-19 activity on different stages of the P. falciparum asexual cycle. Synchronized ring stage parasites (initial parasitaemia 1.4%) were used to yield subcultures, numbered 1–8, which were incubated with DMSO control ( blue line ) or exposed to 100 μM Ned-19 ( red line ) at different times of the parasite asexual cycle. a Stages of the parasite asexual cycle. Black arrows indicate times at which the cultures were sampled. b Histograms showing parasitaemia and distribution of the different parasite stages for the cultures in each time-point. ER early rings, LR late rings, ET early trophozoites, LT late trophozoites, ES early schizonts, LS late schizonts. A representative sample of these forms is shown in Additional file 2 : Figure S2

    Journal: Malaria Journal

    Article Title: Ned-19 inhibition of parasite growth and multiplication suggests a role for NAADP mediated signalling in the asexual development of Plasmodium falciparum

    doi: 10.1186/s12936-017-2013-7

    Figure Lengend Snippet: Ned-19 activity on different stages of the P. falciparum asexual cycle. Synchronized ring stage parasites (initial parasitaemia 1.4%) were used to yield subcultures, numbered 1–8, which were incubated with DMSO control ( blue line ) or exposed to 100 μM Ned-19 ( red line ) at different times of the parasite asexual cycle. a Stages of the parasite asexual cycle. Black arrows indicate times at which the cultures were sampled. b Histograms showing parasitaemia and distribution of the different parasite stages for the cultures in each time-point. ER early rings, LR late rings, ET early trophozoites, LT late trophozoites, ES early schizonts, LS late schizonts. A representative sample of these forms is shown in Additional file 2 : Figure S2

    Article Snippet: Microscopy Parasite cultures were incubated with 200 μM Ned-19 and 1 μM Lysotracker Green DND-26 (ThermoFisher Scientific) for 30 min at 37 °C in agitation and observed with a fluorescence microscope.

    Techniques: Activity Assay, Incubation

    Ned-19 colocalizes with Lysotracker in asexual and sexual parasites. a Parasites stained with 200 μM Ned-19. b Parasites stained with 1 μM Lysotracker. c Parasites stained with 200 μM Ned-19 and 1 μM Lysotracker. BF bright field. Scale bar 5 μm

    Journal: Malaria Journal

    Article Title: Ned-19 inhibition of parasite growth and multiplication suggests a role for NAADP mediated signalling in the asexual development of Plasmodium falciparum

    doi: 10.1186/s12936-017-2013-7

    Figure Lengend Snippet: Ned-19 colocalizes with Lysotracker in asexual and sexual parasites. a Parasites stained with 200 μM Ned-19. b Parasites stained with 1 μM Lysotracker. c Parasites stained with 200 μM Ned-19 and 1 μM Lysotracker. BF bright field. Scale bar 5 μm

    Article Snippet: Microscopy Parasite cultures were incubated with 200 μM Ned-19 and 1 μM Lysotracker Green DND-26 (ThermoFisher Scientific) for 30 min at 37 °C in agitation and observed with a fluorescence microscope.

    Techniques: Staining

    Ned-19 activity on the late phase of the asexual cycle of P. falciparum . Left asexual P. falciparum cultures were synchronized (initial parasitaemia 1.2%) and trophozoites at 32 h post invasion were incubated with DMSO ( blue line ) or 100 μM Ned-19 ( red line ) at the indicated time-points. Right parasitaemia and asexual stages of the cultures 2 h after merozoite egress (determined in the DMSO treated culture). N = 2. *p

    Journal: Malaria Journal

    Article Title: Ned-19 inhibition of parasite growth and multiplication suggests a role for NAADP mediated signalling in the asexual development of Plasmodium falciparum

    doi: 10.1186/s12936-017-2013-7

    Figure Lengend Snippet: Ned-19 activity on the late phase of the asexual cycle of P. falciparum . Left asexual P. falciparum cultures were synchronized (initial parasitaemia 1.2%) and trophozoites at 32 h post invasion were incubated with DMSO ( blue line ) or 100 μM Ned-19 ( red line ) at the indicated time-points. Right parasitaemia and asexual stages of the cultures 2 h after merozoite egress (determined in the DMSO treated culture). N = 2. *p

    Article Snippet: Microscopy Parasite cultures were incubated with 200 μM Ned-19 and 1 μM Lysotracker Green DND-26 (ThermoFisher Scientific) for 30 min at 37 °C in agitation and observed with a fluorescence microscope.

    Techniques: Activity Assay, Incubation

    Ned-19 specific blockage of P. falciparum asexual growth. a Structure of Ned-19 and of its inactive analogue Ned-20, adapted from Rosen et al. b Synchronous early ring stage parasites (initial parasitaemia 0.16%) were cultured for 48 h in the presence of 100 μM concentration of the indicated compounds. At the end of the incubation, parasitaemias were measured through Giemsa-stained preparations. Parasitaemia of the DMSO treated culture (1.08%) was set as 1. N = 3. ***p

    Journal: Malaria Journal

    Article Title: Ned-19 inhibition of parasite growth and multiplication suggests a role for NAADP mediated signalling in the asexual development of Plasmodium falciparum

    doi: 10.1186/s12936-017-2013-7

    Figure Lengend Snippet: Ned-19 specific blockage of P. falciparum asexual growth. a Structure of Ned-19 and of its inactive analogue Ned-20, adapted from Rosen et al. b Synchronous early ring stage parasites (initial parasitaemia 0.16%) were cultured for 48 h in the presence of 100 μM concentration of the indicated compounds. At the end of the incubation, parasitaemias were measured through Giemsa-stained preparations. Parasitaemia of the DMSO treated culture (1.08%) was set as 1. N = 3. ***p

    Article Snippet: Microscopy Parasite cultures were incubated with 200 μM Ned-19 and 1 μM Lysotracker Green DND-26 (ThermoFisher Scientific) for 30 min at 37 °C in agitation and observed with a fluorescence microscope.

    Techniques: Cell Culture, Concentration Assay, Incubation, Staining

    Ned-19 inhibits spontaneous calcium oscillations in P. falciparum early rings and early trophozoites. a Representative traces of spontaneous calcium oscillations in early rings (ER) and early trophozoites (ET) in the presence of Ned-19 or vehicle DMSO. b Bar charts showing the average peak height of the spontaneous calcium oscillations in Ned-19 treated early rings (ER) or early trophozoites (ET) compared to controls. **p

    Journal: Malaria Journal

    Article Title: Ned-19 inhibition of parasite growth and multiplication suggests a role for NAADP mediated signalling in the asexual development of Plasmodium falciparum

    doi: 10.1186/s12936-017-2013-7

    Figure Lengend Snippet: Ned-19 inhibits spontaneous calcium oscillations in P. falciparum early rings and early trophozoites. a Representative traces of spontaneous calcium oscillations in early rings (ER) and early trophozoites (ET) in the presence of Ned-19 or vehicle DMSO. b Bar charts showing the average peak height of the spontaneous calcium oscillations in Ned-19 treated early rings (ER) or early trophozoites (ET) compared to controls. **p

    Article Snippet: Microscopy Parasite cultures were incubated with 200 μM Ned-19 and 1 μM Lysotracker Green DND-26 (ThermoFisher Scientific) for 30 min at 37 °C in agitation and observed with a fluorescence microscope.

    Techniques: