nebnext multiplex oligos for illumina methylated adaptor index primers set 1  (New England Biolabs)


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    NEBNext Multiplex Oligos for Illumina Methylated Adaptor Index Primers Set 1
    Description:
    NEBNext Multiplex Oligos for Illumina Methylated Adaptor Index Primers Set 1 96 rxns
    Catalog Number:
    e7535l
    Price:
    420
    Size:
    96 rxns
    Category:
    DNA Template Preparation for PCR
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    New England Biolabs nebnext multiplex oligos for illumina methylated adaptor index primers set 1
    NEBNext Multiplex Oligos for Illumina Methylated Adaptor Index Primers Set 1
    NEBNext Multiplex Oligos for Illumina Methylated Adaptor Index Primers Set 1 96 rxns
    https://www.bioz.com/result/nebnext multiplex oligos for illumina methylated adaptor index primers set 1/product/New England Biolabs
    Average 99 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    nebnext multiplex oligos for illumina methylated adaptor index primers set 1 - by Bioz Stars, 2020-08
    99/100 stars

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    Related Articles

    Multiplex Assay:

    Article Title: DNA methylation is stable during replication and cell cycle arrest
    Article Snippet: .. Bisulfite converted libraries were PCR amplified and indexed using primers from the NEBNext Multiplex Oligos for Illumina module (New England BioLabs, cat # E7535L) and the Kapa HiFi Uracil+ PCR system (Kapa Biosystems, cat # KK2801). .. PCR enrichment was performed with the following cycling parameters: 98°C for 45 sec followed by 10 cycles at 98°C for 15 sec, 65°C for 30 sec, 72°C for 30 sec and a final extension at 72°C for 1 min.

    Article Title: Comprehensive nucleosome mapping of the human genome in cancer progression
    Article Snippet: .. Universal and indexed sequences were added through 8 cycles of PCR, using NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 1, NEB #E7335S/L). .. The NEBNext® Multiplex Oligos kit contains indices 1-12 which correspond to the identical product if using Illumina® TruSeq primers.

    Article Title: Whole genome amplification with SurePlex results in better copy number alteration detection using sequencing data compared to the MALBAC method
    Article Snippet: .. The size selected DNA samples were then subjected to an enrichment PCR using NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 1 and 2) according to the protocol, with addition of tRNA to minimize the loss of DNA via tube interaction. .. The PCR-free libraries were created entirely according to the TruSeq DNA PCR-free LT sample preparation kit (Illumina), which does not require an enrichment PCR.

    Article Title: Ruler elements in chromatin remodelers set nucleosome array spacing and phasing
    Article Snippet: .. To ligate NEBNext Adaptors (0.75 μM final concentration, NEBNext Multiplex Oligos Kit) to the DNA, samples were incubated with T4 DNA ligase (NEB) at 25 °C for 15 min, followed by incubation with 2 Units USER enzyme (NEB) for 10 min at 37 °C. .. Fragments were purified using 2 volumes AMPure XP beads (Beckman Coulter) and amplified for 8-10 cycles using NEBNext Multiplex Oligos, Phusion High-Fidelity DNA Polymerase (1 U, NEB), deoxynucleotide solution mix (dNTP, 2.5 mM, NEB) and Phusion HF Buffer (1x, NEB).

    Article Title: TET proteins regulate the lineage specification and TCR-mediated expansion of iNKT cells
    Article Snippet: .. Ligation of methylated adapters (NeBNext Multiplex Oligos for Illumina, New England BioLabs) was performed using Quick Ligase Kit (New England BioLabs). .. DNA was bisulfite-treated using the MethylCode Bisulfite Conversion Kit (Life Technologies).

    Amplification:

    Article Title: DNA methylation is stable during replication and cell cycle arrest
    Article Snippet: .. Bisulfite converted libraries were PCR amplified and indexed using primers from the NEBNext Multiplex Oligos for Illumina module (New England BioLabs, cat # E7535L) and the Kapa HiFi Uracil+ PCR system (Kapa Biosystems, cat # KK2801). .. PCR enrichment was performed with the following cycling parameters: 98°C for 45 sec followed by 10 cycles at 98°C for 15 sec, 65°C for 30 sec, 72°C for 30 sec and a final extension at 72°C for 1 min.

    Ligation:

    Article Title: TET proteins regulate the lineage specification and TCR-mediated expansion of iNKT cells
    Article Snippet: .. Ligation of methylated adapters (NeBNext Multiplex Oligos for Illumina, New England BioLabs) was performed using Quick Ligase Kit (New England BioLabs). .. DNA was bisulfite-treated using the MethylCode Bisulfite Conversion Kit (Life Technologies).

    Methylation:

    Article Title: Isoform‐specific localization of DNMT3A regulates DNA methylation fidelity at bivalent CpG islands
    Article Snippet: .. DNA fragments were ligated to methylated adapters (NEB E7535) following the NEB Ultra protocol. .. After adapter removal using Ampure XP beads (Beckman Coulter), DNA was converted using the Qiagen Epitect bisulfite conversion kit following the FFT sample protocol.

    Article Title: TET proteins regulate the lineage specification and TCR-mediated expansion of iNKT cells
    Article Snippet: .. Ligation of methylated adapters (NeBNext Multiplex Oligos for Illumina, New England BioLabs) was performed using Quick Ligase Kit (New England BioLabs). .. DNA was bisulfite-treated using the MethylCode Bisulfite Conversion Kit (Life Technologies).

    Concentration Assay:

    Article Title: Ruler elements in chromatin remodelers set nucleosome array spacing and phasing
    Article Snippet: .. To ligate NEBNext Adaptors (0.75 μM final concentration, NEBNext Multiplex Oligos Kit) to the DNA, samples were incubated with T4 DNA ligase (NEB) at 25 °C for 15 min, followed by incubation with 2 Units USER enzyme (NEB) for 10 min at 37 °C. .. Fragments were purified using 2 volumes AMPure XP beads (Beckman Coulter) and amplified for 8-10 cycles using NEBNext Multiplex Oligos, Phusion High-Fidelity DNA Polymerase (1 U, NEB), deoxynucleotide solution mix (dNTP, 2.5 mM, NEB) and Phusion HF Buffer (1x, NEB).

    Incubation:

    Article Title: Ruler elements in chromatin remodelers set nucleosome array spacing and phasing
    Article Snippet: .. To ligate NEBNext Adaptors (0.75 μM final concentration, NEBNext Multiplex Oligos Kit) to the DNA, samples were incubated with T4 DNA ligase (NEB) at 25 °C for 15 min, followed by incubation with 2 Units USER enzyme (NEB) for 10 min at 37 °C. .. Fragments were purified using 2 volumes AMPure XP beads (Beckman Coulter) and amplified for 8-10 cycles using NEBNext Multiplex Oligos, Phusion High-Fidelity DNA Polymerase (1 U, NEB), deoxynucleotide solution mix (dNTP, 2.5 mM, NEB) and Phusion HF Buffer (1x, NEB).

    Polymerase Chain Reaction:

    Article Title: DNA methylation is stable during replication and cell cycle arrest
    Article Snippet: .. Bisulfite converted libraries were PCR amplified and indexed using primers from the NEBNext Multiplex Oligos for Illumina module (New England BioLabs, cat # E7535L) and the Kapa HiFi Uracil+ PCR system (Kapa Biosystems, cat # KK2801). .. PCR enrichment was performed with the following cycling parameters: 98°C for 45 sec followed by 10 cycles at 98°C for 15 sec, 65°C for 30 sec, 72°C for 30 sec and a final extension at 72°C for 1 min.

    Article Title: Comprehensive nucleosome mapping of the human genome in cancer progression
    Article Snippet: .. Universal and indexed sequences were added through 8 cycles of PCR, using NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 1, NEB #E7335S/L). .. The NEBNext® Multiplex Oligos kit contains indices 1-12 which correspond to the identical product if using Illumina® TruSeq primers.

    Article Title: Whole genome amplification with SurePlex results in better copy number alteration detection using sequencing data compared to the MALBAC method
    Article Snippet: .. The size selected DNA samples were then subjected to an enrichment PCR using NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 1 and 2) according to the protocol, with addition of tRNA to minimize the loss of DNA via tube interaction. .. The PCR-free libraries were created entirely according to the TruSeq DNA PCR-free LT sample preparation kit (Illumina), which does not require an enrichment PCR.

    Sequencing:

    Article Title: Single-cell repertoire tracing identifies rituximab refractory B cells during myasthenia gravis relapses
    Article Snippet: .. An adaptor sequence, containing a universal priming site and a 17-nucleotide unique molecular identifier (UMI) was added to the 3’ end of all cDNA. .. Following purification using streptavidin-coated magnetic beads, PCR was performed to enrich for immunoglobulin sequences using a pool of primers targeting the IGHA, IGHD, IGHE, IGHG, and IGHM regions.

    Article Title: Topoisomerase IIβ targets DNA crossovers formed between distant homologous sites to modulate chromatin structure and gene expression
    Article Snippet: .. We designed the adaptor sequence by modifying the one published elsewhere [ ]. .. Top strand contains a biotinylated deoxy thymidine (Bio-dT) and bottom strand has 3’-dT tail and 5’ overhang of GGCC for the 2nd ligation between adaptors.

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    New England Biolabs nebnext multiplex oligos for illumina
    The newly developed mTSS-Capture method combined with paired-end sequencing maps genome-wide nucleosome distribution in primary patient samples and identifies bona fide nucleosome characteristics, concordant with other human nucleosome mapping studies A . Work-flow of the mTSS-seq method. Following MNase digestion using a titration of MNase, populations of mononucleosomally protected DNA and subnucleosomal fragments are isolated, and prepared as libraries for <t>Illumina</t> sequencing. Solution-based sequence capture is performed using biotinylated <t>oligos,</t> enabling the enrichment of fragments within 2kb of each transcription start site in the human genome. Paired-end 50bp sequencing was then performed on each index. B . Alignment of the mTSS-seq midpoints to the human genome using the UCSC genome browser for LAC patient #4137 Normal tissue is shown for chr11, hg19 ( http://genome.ucsc.edu ). Zooming in twice at 100X allows for further visualization of the sequence capture oligos surrounding the TSS in a 500kb and a 5kb region showing the ATM locus. C . Averaged, normalized reads per million (y-axis) from mTSS-seq plotted as fragments (gray) and midpoints (black), centered on and surrounding 2kb of the TSS for ~22,000 open reading frames in hg19 (x-axis). DNase I-hypersensitivity (GSM736580; green) and RNA polymerase II from ChIP-seq (GSM935299; blue) data from A549 cells are shown. (D) LAC patient 4137 Normal nucleosomal midpoints (blue track) were plotted in the UCSC genome browser against the published human lymphocyte nucleosome distribution maps by Gaffney et. al. (green track) for the ZNF451 and CCDC97 loci. Sequence capture oligos and corresponding RefSeq gene models are shown for each locus. Correlations are shown for ZNF451 and CCDC87, respectively.
    Nebnext Multiplex Oligos For Illumina, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 331 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nebnext multiplex oligos for illumina/product/New England Biolabs
    Average 99 stars, based on 331 article reviews
    Price from $9.99 to $1999.99
    nebnext multiplex oligos for illumina - by Bioz Stars, 2020-08
    99/100 stars
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    The newly developed mTSS-Capture method combined with paired-end sequencing maps genome-wide nucleosome distribution in primary patient samples and identifies bona fide nucleosome characteristics, concordant with other human nucleosome mapping studies A . Work-flow of the mTSS-seq method. Following MNase digestion using a titration of MNase, populations of mononucleosomally protected DNA and subnucleosomal fragments are isolated, and prepared as libraries for Illumina sequencing. Solution-based sequence capture is performed using biotinylated oligos, enabling the enrichment of fragments within 2kb of each transcription start site in the human genome. Paired-end 50bp sequencing was then performed on each index. B . Alignment of the mTSS-seq midpoints to the human genome using the UCSC genome browser for LAC patient #4137 Normal tissue is shown for chr11, hg19 ( http://genome.ucsc.edu ). Zooming in twice at 100X allows for further visualization of the sequence capture oligos surrounding the TSS in a 500kb and a 5kb region showing the ATM locus. C . Averaged, normalized reads per million (y-axis) from mTSS-seq plotted as fragments (gray) and midpoints (black), centered on and surrounding 2kb of the TSS for ~22,000 open reading frames in hg19 (x-axis). DNase I-hypersensitivity (GSM736580; green) and RNA polymerase II from ChIP-seq (GSM935299; blue) data from A549 cells are shown. (D) LAC patient 4137 Normal nucleosomal midpoints (blue track) were plotted in the UCSC genome browser against the published human lymphocyte nucleosome distribution maps by Gaffney et. al. (green track) for the ZNF451 and CCDC97 loci. Sequence capture oligos and corresponding RefSeq gene models are shown for each locus. Correlations are shown for ZNF451 and CCDC87, respectively.

    Journal: Oncotarget

    Article Title: Comprehensive nucleosome mapping of the human genome in cancer progression

    doi: 10.18632/oncotarget.6811

    Figure Lengend Snippet: The newly developed mTSS-Capture method combined with paired-end sequencing maps genome-wide nucleosome distribution in primary patient samples and identifies bona fide nucleosome characteristics, concordant with other human nucleosome mapping studies A . Work-flow of the mTSS-seq method. Following MNase digestion using a titration of MNase, populations of mononucleosomally protected DNA and subnucleosomal fragments are isolated, and prepared as libraries for Illumina sequencing. Solution-based sequence capture is performed using biotinylated oligos, enabling the enrichment of fragments within 2kb of each transcription start site in the human genome. Paired-end 50bp sequencing was then performed on each index. B . Alignment of the mTSS-seq midpoints to the human genome using the UCSC genome browser for LAC patient #4137 Normal tissue is shown for chr11, hg19 ( http://genome.ucsc.edu ). Zooming in twice at 100X allows for further visualization of the sequence capture oligos surrounding the TSS in a 500kb and a 5kb region showing the ATM locus. C . Averaged, normalized reads per million (y-axis) from mTSS-seq plotted as fragments (gray) and midpoints (black), centered on and surrounding 2kb of the TSS for ~22,000 open reading frames in hg19 (x-axis). DNase I-hypersensitivity (GSM736580; green) and RNA polymerase II from ChIP-seq (GSM935299; blue) data from A549 cells are shown. (D) LAC patient 4137 Normal nucleosomal midpoints (blue track) were plotted in the UCSC genome browser against the published human lymphocyte nucleosome distribution maps by Gaffney et. al. (green track) for the ZNF451 and CCDC97 loci. Sequence capture oligos and corresponding RefSeq gene models are shown for each locus. Correlations are shown for ZNF451 and CCDC87, respectively.

    Article Snippet: Universal and indexed sequences were added through 8 cycles of PCR, using NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 1, NEB #E7335S/L).

    Techniques: Sequencing, Genome Wide, Titration, Isolation, Chromatin Immunoprecipitation