nebnext multiplex oligos for illumina index primers set 1  (Illumina Inc)

 
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    Illumina Inc nebnext multiplex oligos for illumina index primers set 1
    Nebnext Multiplex Oligos For Illumina Index Primers Set 1, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nebnext multiplex oligos for illumina index primers set 1/product/Illumina Inc
    Average 92 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    nebnext multiplex oligos for illumina index primers set 1 - by Bioz Stars, 2020-05
    92/100 stars

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    Related Articles

    Multiplex Assay:

    Article Title: Enhancer divergence and cis-regulatory evolution in the human and chimp neural crest
    Article Snippet: .. Sequencing libraries were prepared starting from 30ng of ChIP DNA using the NEBNext Multiplex Oligos for Illumina kit (Cat# E7335S). .. Libraries were multiplexed 4–6 samples per lane for 1×50bp Next-Gen sequencing on Illumina HiSeq platform.

    Article Title: CDK12 controls G1/S progression by regulating RNAPII processivity at core DNA replication genes
    Article Snippet: .. Sequencing libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB, E7760) and NEBNext Multiplex Oligos for Illumina (NEB, E7500S and E7335S) and sequenced with 50 bp at single‐end reads on Illumina HiSeq 2500 (VBCF Vienna, Austria). .. 250 ng of nuclear RNA was used for library preparation after removing ribosomal RNA with NEBNext rRNA Depletion Kit (NEB, E6310S).

    Article Title: Species and cell-type properties of classically defined human and rodent neurons and glia
    Article Snippet: .. Other reagents Optiprep – Sigma D1556-250ML cOmplete mini protease inhibitors (EDTA free) – Roche distributed by Sigma 11836170001 Superasin – Thermo AM2696 RNasin – Promega N2515 16%formaldehyde solution – EMS 15710 BSA – Jackson 001-000-162 BSA RNase free – Thermo AM2618 DyeCycle Ruby – Thermo V10273 Kits Qiagen AllPrep FFPE – Cat #80234 Qiagen MinElute Reaction Cleanup – Cat #28206 Nugen Ovation RNAseq NEBNext Ultra II DNA Library Prep Kit for Illumina - E7645L NEBNext Multiplex Adapters for Illumina – E7335L (Set 1) and E7500L (Set 2) Qubit dsDNA High Sensitivity - Qubit RNA HS - Bioanalyzer Pico chips - 5067–1513 TapeStation D1000 - 5067–5582, ScreenTape; 5067–5583 TapeStation High Sensitivity D1000 – 5067–5584, ScreenTape; 5067–5585 Equipment Dounce homogenizer – 40 mL all glass homogenizer with large and small clearance pestles – Kimble Chase 885300–0040 Ultracentrifuge – Beckman Optima XE, Beckman SW41 Ti swinging rotor and buckets, Beckman Ultraclear 13.2 mL tubes (41121703) Refrigerated benchtop centrifuge similar to Eppendorf 5424R Nutrating Mixer FACS – BD FACSAria with 488, 561, 635/640 lasers Flow cytometry analyzer – BD LSR II with 488, 561, 640 lasers Software – Diva and FlowJo Agilent Bioanalyzer – with pico and nano chips for RNA Agilent TapeStation – D1000 and High Sensitivity D1000 screentapes and reagents Qubit Florometer Roche LightCycler 480 Covaris S220 Focused Ultrasonicator Dynamags from Thermo – 96 side magnet 12331D .. Mice were deeply anesthetized and transcardially perfused with phosphate buffered saline (PBS) followed by 4% formaldehyde (w/v) in PBS.

    Article Title: Species and cell-type properties of classically defined human and rodent neurons and glia
    Article Snippet: .. Other reagents Optiprep – Sigma D1556-250ML cOmplete mini protease inhibitors (EDTA free) – Roche distributed by Sigma 11836170001 Superasin – Thermo AM2696 RNasin – Promega N2515 16%formaldehyde solution – EMS 15710 BSA – Jackson 001-000-162 BSA RNase free – Thermo AM2618 DyeCycle Ruby – Thermo V10273 Kits Qiagen AllPrep FFPE – Cat #80234 Qiagen MinElute Reaction Cleanup – Cat #28206 Nugen Ovation RNAseq NEBNext Ultra II DNA Library Prep Kit for Illumina - E7645L NEBNext Multiplex Adapters for Illumina – E7335L (Set 1) and E7500L (Set 2) Qubit dsDNA High Sensitivity - Q32854 Qubit RNA HS - Q32855 Bioanalyzer Pico chips - 5067–1513 TapeStation D1000 - 5067–5582, ScreenTape; 5067–5583 TapeStation High Sensitivity D1000 – 5067–5584, ScreenTape; 5067–5585 Equipment Dounce homogenizer – 40 mL all glass homogenizer with large and small clearance pestles – Kimble Chase 885300–0040 Ultracentrifuge – Beckman Optima XE, Beckman SW41 Ti swinging rotor and buckets, Beckman Ultraclear 13.2 mL tubes (41121703) Refrigerated benchtop centrifuge similar to Eppendorf 5424R Nutrating Mixer FACS – BD FACSAria with 488, 561, 635/640 lasers Flow cytometry analyzer – BD LSR II with 488, 561, 640 lasers Software – Diva and FlowJo Agilent Bioanalyzer – with pico and nano chips for RNA Agilent TapeStation – D1000 and High Sensitivity D1000 screentapes and reagents Qubit Florometer Roche LightCycler 480 Covaris S220 Focused Ultrasonicator Dynamags from Thermo – 96 side magnet 12331D .. Immunofluorescence Mice were deeply anesthetized and transcardially perfused with phosphate buffered saline (PBS) followed by 4% formaldehyde (w/v) in PBS.

    Article Title: Genome Sequences of RIGVIR Oncolytic Virotherapy Virus and Five Other Echovirus 7 Isolates
    Article Snippet: .. Viral RNA was prepared for sequencing using the NEBNext Ultra RNA library preparation kit (catalog number E7530, New England BioLabs) and NEBNext multiplex oligos for Illumina adapter kit (catalog number E7335) and sequenced on the Illumina MiSeq platform. .. Viral RNA was prepared for sequencing using the NEBNext Ultra RNA library preparation kit (catalog number E7530, New England BioLabs) and NEBNext multiplex oligos for Illumina adapter kit (catalog number E7335) and sequenced on the Illumina MiSeq platform.

    Article Title: Transcriptional Dependencies in Diffuse Intrinsic Pontine Glioma
    Article Snippet: .. Illumina TruSeq Pre-Indexed Adaptors (1uM) or NEBNext Illumina Multiplex Oligo Adaptors (NEB E7335S) were ligated for 1 hour at room temperature. .. Unligated adapters were separated by gel electrophoresis (2.5% agarose, 0.5× TBE) and ligated DNA was purified using a NucleoSpin Gel Clean-up Kit (Macherey-Nagel 740609.250).

    Article Title: Non-inflammatory tumor microenvironment of diffuse intrinsic pontine glioma
    Article Snippet: .. NEBNext Illumina Multiplex Oligo Adaptors (NEB E7335S) were ligated for 1 h at room temperature. .. Unligated adapters were separated by gel electrophoresis (2.5% agarose, 0.5X TBE) and ligated DNA was purified using a NucleoSpin Gel Clean-up Kit (Macherey-Nagel).

    Flow Cytometry:

    Article Title: Species and cell-type properties of classically defined human and rodent neurons and glia
    Article Snippet: .. Other reagents Optiprep – Sigma D1556-250ML cOmplete mini protease inhibitors (EDTA free) – Roche distributed by Sigma 11836170001 Superasin – Thermo AM2696 RNasin – Promega N2515 16%formaldehyde solution – EMS 15710 BSA – Jackson 001-000-162 BSA RNase free – Thermo AM2618 DyeCycle Ruby – Thermo V10273 Kits Qiagen AllPrep FFPE – Cat #80234 Qiagen MinElute Reaction Cleanup – Cat #28206 Nugen Ovation RNAseq NEBNext Ultra II DNA Library Prep Kit for Illumina - E7645L NEBNext Multiplex Adapters for Illumina – E7335L (Set 1) and E7500L (Set 2) Qubit dsDNA High Sensitivity - Qubit RNA HS - Bioanalyzer Pico chips - 5067–1513 TapeStation D1000 - 5067–5582, ScreenTape; 5067–5583 TapeStation High Sensitivity D1000 – 5067–5584, ScreenTape; 5067–5585 Equipment Dounce homogenizer – 40 mL all glass homogenizer with large and small clearance pestles – Kimble Chase 885300–0040 Ultracentrifuge – Beckman Optima XE, Beckman SW41 Ti swinging rotor and buckets, Beckman Ultraclear 13.2 mL tubes (41121703) Refrigerated benchtop centrifuge similar to Eppendorf 5424R Nutrating Mixer FACS – BD FACSAria with 488, 561, 635/640 lasers Flow cytometry analyzer – BD LSR II with 488, 561, 640 lasers Software – Diva and FlowJo Agilent Bioanalyzer – with pico and nano chips for RNA Agilent TapeStation – D1000 and High Sensitivity D1000 screentapes and reagents Qubit Florometer Roche LightCycler 480 Covaris S220 Focused Ultrasonicator Dynamags from Thermo – 96 side magnet 12331D .. Mice were deeply anesthetized and transcardially perfused with phosphate buffered saline (PBS) followed by 4% formaldehyde (w/v) in PBS.

    Article Title: Species and cell-type properties of classically defined human and rodent neurons and glia
    Article Snippet: .. Other reagents Optiprep – Sigma D1556-250ML cOmplete mini protease inhibitors (EDTA free) – Roche distributed by Sigma 11836170001 Superasin – Thermo AM2696 RNasin – Promega N2515 16%formaldehyde solution – EMS 15710 BSA – Jackson 001-000-162 BSA RNase free – Thermo AM2618 DyeCycle Ruby – Thermo V10273 Kits Qiagen AllPrep FFPE – Cat #80234 Qiagen MinElute Reaction Cleanup – Cat #28206 Nugen Ovation RNAseq NEBNext Ultra II DNA Library Prep Kit for Illumina - E7645L NEBNext Multiplex Adapters for Illumina – E7335L (Set 1) and E7500L (Set 2) Qubit dsDNA High Sensitivity - Q32854 Qubit RNA HS - Q32855 Bioanalyzer Pico chips - 5067–1513 TapeStation D1000 - 5067–5582, ScreenTape; 5067–5583 TapeStation High Sensitivity D1000 – 5067–5584, ScreenTape; 5067–5585 Equipment Dounce homogenizer – 40 mL all glass homogenizer with large and small clearance pestles – Kimble Chase 885300–0040 Ultracentrifuge – Beckman Optima XE, Beckman SW41 Ti swinging rotor and buckets, Beckman Ultraclear 13.2 mL tubes (41121703) Refrigerated benchtop centrifuge similar to Eppendorf 5424R Nutrating Mixer FACS – BD FACSAria with 488, 561, 635/640 lasers Flow cytometry analyzer – BD LSR II with 488, 561, 640 lasers Software – Diva and FlowJo Agilent Bioanalyzer – with pico and nano chips for RNA Agilent TapeStation – D1000 and High Sensitivity D1000 screentapes and reagents Qubit Florometer Roche LightCycler 480 Covaris S220 Focused Ultrasonicator Dynamags from Thermo – 96 side magnet 12331D .. Immunofluorescence Mice were deeply anesthetized and transcardially perfused with phosphate buffered saline (PBS) followed by 4% formaldehyde (w/v) in PBS.

    Cytometry:

    Article Title: Species and cell-type properties of classically defined human and rodent neurons and glia
    Article Snippet: .. Other reagents Optiprep – Sigma D1556-250ML cOmplete mini protease inhibitors (EDTA free) – Roche distributed by Sigma 11836170001 Superasin – Thermo AM2696 RNasin – Promega N2515 16%formaldehyde solution – EMS 15710 BSA – Jackson 001-000-162 BSA RNase free – Thermo AM2618 DyeCycle Ruby – Thermo V10273 Kits Qiagen AllPrep FFPE – Cat #80234 Qiagen MinElute Reaction Cleanup – Cat #28206 Nugen Ovation RNAseq NEBNext Ultra II DNA Library Prep Kit for Illumina - E7645L NEBNext Multiplex Adapters for Illumina – E7335L (Set 1) and E7500L (Set 2) Qubit dsDNA High Sensitivity - Qubit RNA HS - Bioanalyzer Pico chips - 5067–1513 TapeStation D1000 - 5067–5582, ScreenTape; 5067–5583 TapeStation High Sensitivity D1000 – 5067–5584, ScreenTape; 5067–5585 Equipment Dounce homogenizer – 40 mL all glass homogenizer with large and small clearance pestles – Kimble Chase 885300–0040 Ultracentrifuge – Beckman Optima XE, Beckman SW41 Ti swinging rotor and buckets, Beckman Ultraclear 13.2 mL tubes (41121703) Refrigerated benchtop centrifuge similar to Eppendorf 5424R Nutrating Mixer FACS – BD FACSAria with 488, 561, 635/640 lasers Flow cytometry analyzer – BD LSR II with 488, 561, 640 lasers Software – Diva and FlowJo Agilent Bioanalyzer – with pico and nano chips for RNA Agilent TapeStation – D1000 and High Sensitivity D1000 screentapes and reagents Qubit Florometer Roche LightCycler 480 Covaris S220 Focused Ultrasonicator Dynamags from Thermo – 96 side magnet 12331D .. Mice were deeply anesthetized and transcardially perfused with phosphate buffered saline (PBS) followed by 4% formaldehyde (w/v) in PBS.

    Article Title: Species and cell-type properties of classically defined human and rodent neurons and glia
    Article Snippet: .. Other reagents Optiprep – Sigma D1556-250ML cOmplete mini protease inhibitors (EDTA free) – Roche distributed by Sigma 11836170001 Superasin – Thermo AM2696 RNasin – Promega N2515 16%formaldehyde solution – EMS 15710 BSA – Jackson 001-000-162 BSA RNase free – Thermo AM2618 DyeCycle Ruby – Thermo V10273 Kits Qiagen AllPrep FFPE – Cat #80234 Qiagen MinElute Reaction Cleanup – Cat #28206 Nugen Ovation RNAseq NEBNext Ultra II DNA Library Prep Kit for Illumina - E7645L NEBNext Multiplex Adapters for Illumina – E7335L (Set 1) and E7500L (Set 2) Qubit dsDNA High Sensitivity - Q32854 Qubit RNA HS - Q32855 Bioanalyzer Pico chips - 5067–1513 TapeStation D1000 - 5067–5582, ScreenTape; 5067–5583 TapeStation High Sensitivity D1000 – 5067–5584, ScreenTape; 5067–5585 Equipment Dounce homogenizer – 40 mL all glass homogenizer with large and small clearance pestles – Kimble Chase 885300–0040 Ultracentrifuge – Beckman Optima XE, Beckman SW41 Ti swinging rotor and buckets, Beckman Ultraclear 13.2 mL tubes (41121703) Refrigerated benchtop centrifuge similar to Eppendorf 5424R Nutrating Mixer FACS – BD FACSAria with 488, 561, 635/640 lasers Flow cytometry analyzer – BD LSR II with 488, 561, 640 lasers Software – Diva and FlowJo Agilent Bioanalyzer – with pico and nano chips for RNA Agilent TapeStation – D1000 and High Sensitivity D1000 screentapes and reagents Qubit Florometer Roche LightCycler 480 Covaris S220 Focused Ultrasonicator Dynamags from Thermo – 96 side magnet 12331D .. Immunofluorescence Mice were deeply anesthetized and transcardially perfused with phosphate buffered saline (PBS) followed by 4% formaldehyde (w/v) in PBS.

    Microarray:

    Article Title: β-Catenin maintains lung epithelial progenitors after lung specification
    Article Snippet: .. The libraries were bar-coded (New England BioLabs, E7335S) and sequenced on an Illumina HiSeq 4000 or NextSeq 500 at the UT MD Anderson Sequencing and Microarray Facility to obtain 20 million-30 million 76-nucleotide pair-end reads for each sample. ..

    Formalin-fixed Paraffin-Embedded:

    Article Title: Species and cell-type properties of classically defined human and rodent neurons and glia
    Article Snippet: .. Other reagents Optiprep – Sigma D1556-250ML cOmplete mini protease inhibitors (EDTA free) – Roche distributed by Sigma 11836170001 Superasin – Thermo AM2696 RNasin – Promega N2515 16%formaldehyde solution – EMS 15710 BSA – Jackson 001-000-162 BSA RNase free – Thermo AM2618 DyeCycle Ruby – Thermo V10273 Kits Qiagen AllPrep FFPE – Cat #80234 Qiagen MinElute Reaction Cleanup – Cat #28206 Nugen Ovation RNAseq NEBNext Ultra II DNA Library Prep Kit for Illumina - E7645L NEBNext Multiplex Adapters for Illumina – E7335L (Set 1) and E7500L (Set 2) Qubit dsDNA High Sensitivity - Qubit RNA HS - Bioanalyzer Pico chips - 5067–1513 TapeStation D1000 - 5067–5582, ScreenTape; 5067–5583 TapeStation High Sensitivity D1000 – 5067–5584, ScreenTape; 5067–5585 Equipment Dounce homogenizer – 40 mL all glass homogenizer with large and small clearance pestles – Kimble Chase 885300–0040 Ultracentrifuge – Beckman Optima XE, Beckman SW41 Ti swinging rotor and buckets, Beckman Ultraclear 13.2 mL tubes (41121703) Refrigerated benchtop centrifuge similar to Eppendorf 5424R Nutrating Mixer FACS – BD FACSAria with 488, 561, 635/640 lasers Flow cytometry analyzer – BD LSR II with 488, 561, 640 lasers Software – Diva and FlowJo Agilent Bioanalyzer – with pico and nano chips for RNA Agilent TapeStation – D1000 and High Sensitivity D1000 screentapes and reagents Qubit Florometer Roche LightCycler 480 Covaris S220 Focused Ultrasonicator Dynamags from Thermo – 96 side magnet 12331D .. Mice were deeply anesthetized and transcardially perfused with phosphate buffered saline (PBS) followed by 4% formaldehyde (w/v) in PBS.

    Article Title: Species and cell-type properties of classically defined human and rodent neurons and glia
    Article Snippet: .. Other reagents Optiprep – Sigma D1556-250ML cOmplete mini protease inhibitors (EDTA free) – Roche distributed by Sigma 11836170001 Superasin – Thermo AM2696 RNasin – Promega N2515 16%formaldehyde solution – EMS 15710 BSA – Jackson 001-000-162 BSA RNase free – Thermo AM2618 DyeCycle Ruby – Thermo V10273 Kits Qiagen AllPrep FFPE – Cat #80234 Qiagen MinElute Reaction Cleanup – Cat #28206 Nugen Ovation RNAseq NEBNext Ultra II DNA Library Prep Kit for Illumina - E7645L NEBNext Multiplex Adapters for Illumina – E7335L (Set 1) and E7500L (Set 2) Qubit dsDNA High Sensitivity - Q32854 Qubit RNA HS - Q32855 Bioanalyzer Pico chips - 5067–1513 TapeStation D1000 - 5067–5582, ScreenTape; 5067–5583 TapeStation High Sensitivity D1000 – 5067–5584, ScreenTape; 5067–5585 Equipment Dounce homogenizer – 40 mL all glass homogenizer with large and small clearance pestles – Kimble Chase 885300–0040 Ultracentrifuge – Beckman Optima XE, Beckman SW41 Ti swinging rotor and buckets, Beckman Ultraclear 13.2 mL tubes (41121703) Refrigerated benchtop centrifuge similar to Eppendorf 5424R Nutrating Mixer FACS – BD FACSAria with 488, 561, 635/640 lasers Flow cytometry analyzer – BD LSR II with 488, 561, 640 lasers Software – Diva and FlowJo Agilent Bioanalyzer – with pico and nano chips for RNA Agilent TapeStation – D1000 and High Sensitivity D1000 screentapes and reagents Qubit Florometer Roche LightCycler 480 Covaris S220 Focused Ultrasonicator Dynamags from Thermo – 96 side magnet 12331D .. Immunofluorescence Mice were deeply anesthetized and transcardially perfused with phosphate buffered saline (PBS) followed by 4% formaldehyde (w/v) in PBS.

    Sequencing:

    Article Title: Enhancer divergence and cis-regulatory evolution in the human and chimp neural crest
    Article Snippet: .. Sequencing libraries were prepared starting from 30ng of ChIP DNA using the NEBNext Multiplex Oligos for Illumina kit (Cat# E7335S). .. Libraries were multiplexed 4–6 samples per lane for 1×50bp Next-Gen sequencing on Illumina HiSeq platform.

    Article Title: CDK12 controls G1/S progression by regulating RNAPII processivity at core DNA replication genes
    Article Snippet: .. Sequencing libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB, E7760) and NEBNext Multiplex Oligos for Illumina (NEB, E7500S and E7335S) and sequenced with 50 bp at single‐end reads on Illumina HiSeq 2500 (VBCF Vienna, Austria). .. 250 ng of nuclear RNA was used for library preparation after removing ribosomal RNA with NEBNext rRNA Depletion Kit (NEB, E6310S).

    Article Title: β-Catenin maintains lung epithelial progenitors after lung specification
    Article Snippet: .. The libraries were bar-coded (New England BioLabs, E7335S) and sequenced on an Illumina HiSeq 4000 or NextSeq 500 at the UT MD Anderson Sequencing and Microarray Facility to obtain 20 million-30 million 76-nucleotide pair-end reads for each sample. ..

    Article Title: Genome Sequences of RIGVIR Oncolytic Virotherapy Virus and Five Other Echovirus 7 Isolates
    Article Snippet: .. Viral RNA was prepared for sequencing using the NEBNext Ultra RNA library preparation kit (catalog number E7530, New England BioLabs) and NEBNext multiplex oligos for Illumina adapter kit (catalog number E7335) and sequenced on the Illumina MiSeq platform. .. Viral RNA was prepared for sequencing using the NEBNext Ultra RNA library preparation kit (catalog number E7530, New England BioLabs) and NEBNext multiplex oligos for Illumina adapter kit (catalog number E7335) and sequenced on the Illumina MiSeq platform.

    FACS:

    Article Title: Species and cell-type properties of classically defined human and rodent neurons and glia
    Article Snippet: .. Other reagents Optiprep – Sigma D1556-250ML cOmplete mini protease inhibitors (EDTA free) – Roche distributed by Sigma 11836170001 Superasin – Thermo AM2696 RNasin – Promega N2515 16%formaldehyde solution – EMS 15710 BSA – Jackson 001-000-162 BSA RNase free – Thermo AM2618 DyeCycle Ruby – Thermo V10273 Kits Qiagen AllPrep FFPE – Cat #80234 Qiagen MinElute Reaction Cleanup – Cat #28206 Nugen Ovation RNAseq NEBNext Ultra II DNA Library Prep Kit for Illumina - E7645L NEBNext Multiplex Adapters for Illumina – E7335L (Set 1) and E7500L (Set 2) Qubit dsDNA High Sensitivity - Qubit RNA HS - Bioanalyzer Pico chips - 5067–1513 TapeStation D1000 - 5067–5582, ScreenTape; 5067–5583 TapeStation High Sensitivity D1000 – 5067–5584, ScreenTape; 5067–5585 Equipment Dounce homogenizer – 40 mL all glass homogenizer with large and small clearance pestles – Kimble Chase 885300–0040 Ultracentrifuge – Beckman Optima XE, Beckman SW41 Ti swinging rotor and buckets, Beckman Ultraclear 13.2 mL tubes (41121703) Refrigerated benchtop centrifuge similar to Eppendorf 5424R Nutrating Mixer FACS – BD FACSAria with 488, 561, 635/640 lasers Flow cytometry analyzer – BD LSR II with 488, 561, 640 lasers Software – Diva and FlowJo Agilent Bioanalyzer – with pico and nano chips for RNA Agilent TapeStation – D1000 and High Sensitivity D1000 screentapes and reagents Qubit Florometer Roche LightCycler 480 Covaris S220 Focused Ultrasonicator Dynamags from Thermo – 96 side magnet 12331D .. Mice were deeply anesthetized and transcardially perfused with phosphate buffered saline (PBS) followed by 4% formaldehyde (w/v) in PBS.

    Article Title: Species and cell-type properties of classically defined human and rodent neurons and glia
    Article Snippet: .. Other reagents Optiprep – Sigma D1556-250ML cOmplete mini protease inhibitors (EDTA free) – Roche distributed by Sigma 11836170001 Superasin – Thermo AM2696 RNasin – Promega N2515 16%formaldehyde solution – EMS 15710 BSA – Jackson 001-000-162 BSA RNase free – Thermo AM2618 DyeCycle Ruby – Thermo V10273 Kits Qiagen AllPrep FFPE – Cat #80234 Qiagen MinElute Reaction Cleanup – Cat #28206 Nugen Ovation RNAseq NEBNext Ultra II DNA Library Prep Kit for Illumina - E7645L NEBNext Multiplex Adapters for Illumina – E7335L (Set 1) and E7500L (Set 2) Qubit dsDNA High Sensitivity - Q32854 Qubit RNA HS - Q32855 Bioanalyzer Pico chips - 5067–1513 TapeStation D1000 - 5067–5582, ScreenTape; 5067–5583 TapeStation High Sensitivity D1000 – 5067–5584, ScreenTape; 5067–5585 Equipment Dounce homogenizer – 40 mL all glass homogenizer with large and small clearance pestles – Kimble Chase 885300–0040 Ultracentrifuge – Beckman Optima XE, Beckman SW41 Ti swinging rotor and buckets, Beckman Ultraclear 13.2 mL tubes (41121703) Refrigerated benchtop centrifuge similar to Eppendorf 5424R Nutrating Mixer FACS – BD FACSAria with 488, 561, 635/640 lasers Flow cytometry analyzer – BD LSR II with 488, 561, 640 lasers Software – Diva and FlowJo Agilent Bioanalyzer – with pico and nano chips for RNA Agilent TapeStation – D1000 and High Sensitivity D1000 screentapes and reagents Qubit Florometer Roche LightCycler 480 Covaris S220 Focused Ultrasonicator Dynamags from Thermo – 96 side magnet 12331D .. Immunofluorescence Mice were deeply anesthetized and transcardially perfused with phosphate buffered saline (PBS) followed by 4% formaldehyde (w/v) in PBS.

    Chromatin Immunoprecipitation:

    Article Title: Enhancer divergence and cis-regulatory evolution in the human and chimp neural crest
    Article Snippet: .. Sequencing libraries were prepared starting from 30ng of ChIP DNA using the NEBNext Multiplex Oligos for Illumina kit (Cat# E7335S). .. Libraries were multiplexed 4–6 samples per lane for 1×50bp Next-Gen sequencing on Illumina HiSeq platform.

    Software:

    Article Title: Species and cell-type properties of classically defined human and rodent neurons and glia
    Article Snippet: .. Other reagents Optiprep – Sigma D1556-250ML cOmplete mini protease inhibitors (EDTA free) – Roche distributed by Sigma 11836170001 Superasin – Thermo AM2696 RNasin – Promega N2515 16%formaldehyde solution – EMS 15710 BSA – Jackson 001-000-162 BSA RNase free – Thermo AM2618 DyeCycle Ruby – Thermo V10273 Kits Qiagen AllPrep FFPE – Cat #80234 Qiagen MinElute Reaction Cleanup – Cat #28206 Nugen Ovation RNAseq NEBNext Ultra II DNA Library Prep Kit for Illumina - E7645L NEBNext Multiplex Adapters for Illumina – E7335L (Set 1) and E7500L (Set 2) Qubit dsDNA High Sensitivity - Qubit RNA HS - Bioanalyzer Pico chips - 5067–1513 TapeStation D1000 - 5067–5582, ScreenTape; 5067–5583 TapeStation High Sensitivity D1000 – 5067–5584, ScreenTape; 5067–5585 Equipment Dounce homogenizer – 40 mL all glass homogenizer with large and small clearance pestles – Kimble Chase 885300–0040 Ultracentrifuge – Beckman Optima XE, Beckman SW41 Ti swinging rotor and buckets, Beckman Ultraclear 13.2 mL tubes (41121703) Refrigerated benchtop centrifuge similar to Eppendorf 5424R Nutrating Mixer FACS – BD FACSAria with 488, 561, 635/640 lasers Flow cytometry analyzer – BD LSR II with 488, 561, 640 lasers Software – Diva and FlowJo Agilent Bioanalyzer – with pico and nano chips for RNA Agilent TapeStation – D1000 and High Sensitivity D1000 screentapes and reagents Qubit Florometer Roche LightCycler 480 Covaris S220 Focused Ultrasonicator Dynamags from Thermo – 96 side magnet 12331D .. Mice were deeply anesthetized and transcardially perfused with phosphate buffered saline (PBS) followed by 4% formaldehyde (w/v) in PBS.

    Article Title: Species and cell-type properties of classically defined human and rodent neurons and glia
    Article Snippet: .. Other reagents Optiprep – Sigma D1556-250ML cOmplete mini protease inhibitors (EDTA free) – Roche distributed by Sigma 11836170001 Superasin – Thermo AM2696 RNasin – Promega N2515 16%formaldehyde solution – EMS 15710 BSA – Jackson 001-000-162 BSA RNase free – Thermo AM2618 DyeCycle Ruby – Thermo V10273 Kits Qiagen AllPrep FFPE – Cat #80234 Qiagen MinElute Reaction Cleanup – Cat #28206 Nugen Ovation RNAseq NEBNext Ultra II DNA Library Prep Kit for Illumina - E7645L NEBNext Multiplex Adapters for Illumina – E7335L (Set 1) and E7500L (Set 2) Qubit dsDNA High Sensitivity - Q32854 Qubit RNA HS - Q32855 Bioanalyzer Pico chips - 5067–1513 TapeStation D1000 - 5067–5582, ScreenTape; 5067–5583 TapeStation High Sensitivity D1000 – 5067–5584, ScreenTape; 5067–5585 Equipment Dounce homogenizer – 40 mL all glass homogenizer with large and small clearance pestles – Kimble Chase 885300–0040 Ultracentrifuge – Beckman Optima XE, Beckman SW41 Ti swinging rotor and buckets, Beckman Ultraclear 13.2 mL tubes (41121703) Refrigerated benchtop centrifuge similar to Eppendorf 5424R Nutrating Mixer FACS – BD FACSAria with 488, 561, 635/640 lasers Flow cytometry analyzer – BD LSR II with 488, 561, 640 lasers Software – Diva and FlowJo Agilent Bioanalyzer – with pico and nano chips for RNA Agilent TapeStation – D1000 and High Sensitivity D1000 screentapes and reagents Qubit Florometer Roche LightCycler 480 Covaris S220 Focused Ultrasonicator Dynamags from Thermo – 96 side magnet 12331D .. Immunofluorescence Mice were deeply anesthetized and transcardially perfused with phosphate buffered saline (PBS) followed by 4% formaldehyde (w/v) in PBS.

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    Illumina Inc nebnext multiplex oligos for illumina index primers set 1
    Nebnext Multiplex Oligos For Illumina Index Primers Set 1, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nebnext multiplex oligos for illumina index primers set 1/product/Illumina Inc
    Average 92 stars, based on 2 article reviews
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